COMMENT ARTICLE 1

Systemic administration of TAT-GRpep

Institution

Name

Date
COMMENT ARTICLE 2

According to figure 2, Fear-conditioned mice had considerably more significant amounts

of the GR-FKBP51 complex in their brain tissues. According to Coimmunoprecipitation, fear-

conditioned mouse brain lysate has more substantial portions of the GR-FKBP51 complex than

control (CTRL) animals. Due to the usage of Coimmunoprecipitation, it depicts that compared to

the control mice, the brain lysate of fear-conditioned mice appeared to show higher levels of GR-

FKBP51. In (A), GR antibody inclines to precipitates FKBP51 and GR through the

representative western blot. While in (B), after Coimmunoprecipitation through GR antibody in

the brain lysate of regulator or fright-habituated mice, it appeared to influence the levels of

FKBP51 by the Densitometric analysis. (Li et al, 2020). Following division through the quantity

of triggered GR (GR IP), the Coimmunoprecipitated FKBP51 (FKBP51 Co-IP) group returned

normal.

The proportion of control is used to indicate the outcomes for every experiment (CTRL).

Co-IP 0.001 against specimen, t-test; n = 7. The statistics are presented as an average SEM. In

(C), it elaborates the graphic investigational program concerning information in D. further, in

(D), the animals from both teams tend to demonstrate a simulated absenteeism of icing

performance (P=NS) throughout the 3-minute acclimatization stage (absence of CS). (Li et al,

2020). Nevertheless, livestock in the management peptide group immobilized below the

mechanism peptide (TAT) cluster n=10; (P<0.05), just after the cues were presented. Likewise,

in (E), it demonstrates schematically the investigational program concerning information given

in F. Regarding (F), mice cued freezing performance were decreased by the use of n= 14 (TAT);

TAT-GRpep (P<0.05).
COMMENT ARTICLE 3

Reference

Li, H., Su, P., Lai, T. K., Jiang, A., Liu, J., Zhai, D., ... & Liu, F. (2020). The glucocorticoid

receptor–FKBP51 complex contributes to fear conditioning and posttraumatic stress

disorder. The Journal of clinical investigation, 130(2), 877-889.