Protein determination using Bradford method

Samples Samples for the calibration curve Unknown sample Blank

BSA 1 BSA 2 BSA 3 BSA 4 BSA 5
Reagents, μl 0,2 mg/ml 0,4 mg/ml 0,6 mg/ml 0,8 mg/ml 1,0 mg/ml
BSA 0,2 mg/ml 50 - - - - - -
BSA 0,4 mg/ml - 50 - - - - -
BSA 0,6 mg/ml - - 50 - - - -
BSA 0,8 mg/ml - - - 50 - - -
BSA 1,0 mg/ml - - - - 50 - -
Unknown sample - - - - - 50 -
H2O - - - - - - 50
Bradford reagent 1500 1500 1500 1500 1500 1500 1500

Absorbance values 0.092 0.211 0.455 0.561 0.791 0.497 -

(λmax=595 nm)

0.9
0.8
0.7
f(x) = 0.73 x
0.6
Absorbanta

0.5
0.4
0.3
0.2
0.1
0
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 1.1

Concentratie, mg/ml