BGL when

tremors
and
x y y y
convulsio anima

B lo o d g lu co se lev el (m g /d L)
ns ceased
BGL when tremors and convulsions were unfasted
Animal BGL just before insulin injection after
observed + glucose 8
glucose or
4
Unfasted + glucose 89 18 epinephri
83 BGL before 89
Unfasted + epinephrine 91 16 ne 87 BGL when t 18
administra
Fasted + glucose 44 10 tion 76 BGL when t 83
Fasted + epinephrine 47 13 79

Animal Behavior before insulin injection Behavior after insulin injection Tim

Unfasted + glucose docile, walking inside the cage running inside the cage unfasted + glucose
Unfasted + epinephrine docile, walking inside the cage running inside the cage fasted + glucose

Fasted + glucose running inside the cage running inside the cage
Fasted + epinephrine running inside the cage running inside the cage
 animal
unfasted a fasted +
+ fasted +
epinephri
epinephri glucose
80
ne
ne 40
91 44 47
0
16 10 13
87 76 79

Time of observation

unfasted + glucose unfasted + epinephrine

fasted + glucose fasted + epinephrine
x Mouse A Mouse B Mouse C Mouse D
0 7.954 7.89 7.799 7.892
24 18.673 10.145 10.977 9.875
48 23.576 8.213 8.675 7.983

Mouse A Mouse B
N E B L M N
prior to injection of the irritant 29 10 0 51 10 27
after 24 hours 45 20 0 27 8 35
after 48 hours 55 14 0 27 8 30

0 24
N E B L M N
Mouse A 29 10 0 51 10 45
Mouse B 27 11 0 53 9 35
Mouse C 26 8 0 55 10 41
Mouse D 26 12 0 54 8 35

0 24
Mouse A Mouse B Mouse C Mouse D Mouse A Mouse B
Neutrophils 29 27 26 26 45 35
Eosinophils 10 11 8 12 20 16
Basophils 0 0 0 0 0 0
Lymphocytes 51 53 55 54 27 39
Monocytes 10 9 10 8 8 10
sum 100 100 99 100 100 100

60

50
Differential WBC Count (%)

40

30

20

10

0
Mouse C
Mouse D
Mouse C

Mouse C

Mouse D

Mouse D
Mouse A

Mouse A
Mouse A

Mouse B

Mouse B
Mouse B
0

24

48

Time of observation (hrs)

Neutrophils Eosinophils Basophils Lymphocytes Monocytes

Chart Title
120

100

80

60

40

20
 Chart Title
120

100

80

60

40

20

0
Mouse Mouse Mouse Mouse Mouse Mouse Mouse Mouse Mouse Mouse Mouse Mouse
A B C D A B C D A B C D
0 24 48

Neutrophils Eosinophils Basophils Lymphocytes Monocytes

 Mouse B Mouse C Mo
E B L M N E B L M N
11 0 53 9 26 8 0 55 10 26
16 0 39 10 41 10 0 40 8 35
13 0 53 10 46 10 0 45 8 29

24 48
E B L M N E B L M
20 0 27 8 55 14 0 27 8
16 0 39 10 30 13 0 53 10
10 0 40 8 46 10 0 45 8
14 0 43 6 29 12 0 51 8

24 48
Mouse C Mouse D Mouse A Mouse B Mouse C Mouse D
41 35 55 30 46 29
10 14 14 13 10 12
0 0 0 0 0 0
40 43 27 53 45 51
8 6 8 10 8 8
99 98 104 106 109 100

60 60

50 50
Differential WBC Count (%)

40 40
Axis Title

30
30
20
20
10
10
0
0 N E B L M N E B L
N E B L M N E B L M N E B L M Mouse A Mouse B
0 24 48
Time of observation (hrs)

prior to injection of th
Mouse A Mouse B Mouse C Mouse D after 48 hours
 25

20

WBC couunt (x103/µL)

15
Mouse D
E B L M 10

12 0 54 8
5
14 0 43 6
12 0 51 8
0
0 24
Time of observation (hrs)

Mouse A Mouse B Mouse C Mouse D

b4>>

E B L M N E B L M N E B L M N E B L M
Mouse A Mouse B Mouse C Mouse D
Axis Title

prior to injection of the irritant after 24 hours

after 48 hours
24 48
rvation (hrs)

Mouse C Mouse D