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Republic of the Philippines

CENTRAL BICOL STATE UNIVERSITY OF AGRICULTURE


San Jose, Pili, Camarines Sur 4418
ISO 9001:2015 www.cbsua.edu.ph
TÜV - R 01 100 1934918

Answer the following:


1. Which type of microscope would be best to use to observe each of the
following?
a. a stained bacterial smear
b. unstained bacterial cells: the cells are small, and no detail is needed
c. unstained live tissue when it is desirable to see some intracellular
detail
d. a sample that emits light when illuminated with ultraviolet light
e. intracellular detail of a cell that is 1 μm long
f. unstained live cells in which intracellular structures are shown in color
2. Calculate the total magnification of the nucleus of a cell being observed
through a compound light microscope with a 10* ocular lens and an oil
immersion lens.
3. The maximum magnification of a compound microscope is (a)______;
that of an electron microscope, (b)________. The maximum resolution
of a compound microscope is (c)_______; that of an electron
microscope, (d)______. One advantage of a scanning electron
microscope over a transmission electron microscope is (e) ______.
4. Why is immersion oil necessary at 1000x but not with the lower power
objectives?
Discussion Board
1. Through what lenses does light pass in a compound microscope?
2. What does it mean when a microscope has a resolution of 0.2 nm?
3. What are the advantages of brightfield, darkfield, and phase-contrast
microscopy?
4. Why is a DIC micrograph brightly colored?
5. Why won’t other bacteria fluoresce in the FTA-ABS test?
6. What are the advantages of confocal microscopy?
7. What are the differences between TPM and confocal microscopy?
8. How are brightfield, darkfield, phase-contrast, and fluorescence
microscopy similar?
9. What is the advantage of super-resolution light microscopy?
10. What is the principal use of SAM?
11. How do TEM and SEM images of the same organism differ?
12. Why do electron microscopes have greater resolution than light
microscopes?
13. What is the principle employed in scanned-probe microscopy?
Post-Competency Checklist
Online Quiz (TBA)

BIO 102 MICROBIOLOGY AND PARASITOLOGY 11

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