This document contains questions about different types of microscopes and their uses and capabilities. It asks about which microscope would be best for observing stained bacterial smears, unstained cells, live tissue, and samples that emit light under UV illumination. It also contains questions about calculating total microscope magnification, the maximum magnifications and resolutions of compound, electron, and scanning electron microscopes, and why immersion oil is needed at high powers. Discussion questions cover light paths in compound microscopes, resolution, advantages of different microscopy techniques, confocal vs total internal reflection microscopy, similarities between types of light microscopy, super-resolution techniques, the uses of scanning acoustic microscopy, differences between TEM and SEM images, why electron microscopes have higher resolution,
This document contains questions about different types of microscopes and their uses and capabilities. It asks about which microscope would be best for observing stained bacterial smears, unstained cells, live tissue, and samples that emit light under UV illumination. It also contains questions about calculating total microscope magnification, the maximum magnifications and resolutions of compound, electron, and scanning electron microscopes, and why immersion oil is needed at high powers. Discussion questions cover light paths in compound microscopes, resolution, advantages of different microscopy techniques, confocal vs total internal reflection microscopy, similarities between types of light microscopy, super-resolution techniques, the uses of scanning acoustic microscopy, differences between TEM and SEM images, why electron microscopes have higher resolution,
This document contains questions about different types of microscopes and their uses and capabilities. It asks about which microscope would be best for observing stained bacterial smears, unstained cells, live tissue, and samples that emit light under UV illumination. It also contains questions about calculating total microscope magnification, the maximum magnifications and resolutions of compound, electron, and scanning electron microscopes, and why immersion oil is needed at high powers. Discussion questions cover light paths in compound microscopes, resolution, advantages of different microscopy techniques, confocal vs total internal reflection microscopy, similarities between types of light microscopy, super-resolution techniques, the uses of scanning acoustic microscopy, differences between TEM and SEM images, why electron microscopes have higher resolution,
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Answer the following:
1. Which type of microscope would be best to use to observe each of the following? a. a stained bacterial smear b. unstained bacterial cells: the cells are small, and no detail is needed c. unstained live tissue when it is desirable to see some intracellular detail d. a sample that emits light when illuminated with ultraviolet light e. intracellular detail of a cell that is 1 μm long f. unstained live cells in which intracellular structures are shown in color 2. Calculate the total magnification of the nucleus of a cell being observed through a compound light microscope with a 10* ocular lens and an oil immersion lens. 3. The maximum magnification of a compound microscope is (a)______; that of an electron microscope, (b)________. The maximum resolution of a compound microscope is (c)_______; that of an electron microscope, (d)______. One advantage of a scanning electron microscope over a transmission electron microscope is (e) ______. 4. Why is immersion oil necessary at 1000x but not with the lower power objectives? Discussion Board 1. Through what lenses does light pass in a compound microscope? 2. What does it mean when a microscope has a resolution of 0.2 nm? 3. What are the advantages of brightfield, darkfield, and phase-contrast microscopy? 4. Why is a DIC micrograph brightly colored? 5. Why won’t other bacteria fluoresce in the FTA-ABS test? 6. What are the advantages of confocal microscopy? 7. What are the differences between TPM and confocal microscopy? 8. How are brightfield, darkfield, phase-contrast, and fluorescence microscopy similar? 9. What is the advantage of super-resolution light microscopy? 10. What is the principal use of SAM? 11. How do TEM and SEM images of the same organism differ? 12. Why do electron microscopes have greater resolution than light microscopes? 13. What is the principle employed in scanned-probe microscopy? Post-Competency Checklist Online Quiz (TBA)