Jurnal Iktiologi Indonesia 

Kandungan mikroplastik pada empat jenis ikan ekonomis penting

di perairan Selat Bali
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 Kandungan mikroplastik pada empat jenis ikan

Prosedur analisis laboratorium penambahan N-Hexane. Pada tahap ini, sampel

Analisis laboratorium yang digunakan pada
penelitian ini merupakan modifikasi
beberapa tahapan dalam penelitian
dilakukan oleh Jabeen et al. (2017) dan Masura
et al. (2015). Beberapa tahapan yang diadopsi
dan dimodifikasi meliputi preparasi sampel
ikan, penggunaan volume larutan H2O2 30% dan
Fe (II) 0,05 M yang digunakan, serta dilakukan
modifikasi pada penyaringan sampel dengan
menghilangkan tahapan floatasi.
floatasi dilakukan untuk memisahkan partikel
mikroplastik dengan densitas yang lebih rendah
dibandingkan densitas air dan bahan organik
(Coppock et al. 2017). Hal ini biasanya di-
lakukan dengan menambahkan larutan garam
untuk menambah densitas air. Prosedur analisis
laboratorium pada penelitian ini terbagi menjadi
beberapa tahap, yaitu preparasi
perlakuan hidrogen peroksida, identifikasi
mikroplastik dan uji FTIR.
Preparasi sampel
Tahap preparasi sampel ikan dilakukan
dengan menggunakan metode Jabeen et al.
(2017) yaitu berupa pengambilan sampel pada
usus dan lambung. Preparasi sampel meliputi
persiapan alat dan bahan, serta pembedahan ikan
untuk diambil saluran pencernaannya. Ikan yang
beku didiamkan pada suhu ruang dan pem-
bedahan dilakukan untuk mengambil bagian
usus dan lambung ikan. Selanjutnya pencernaan
ikan ditimbang sebagai berat basah sampel.
yang sudah disiapkan dalam wadah ditam-
dari bahkan 20 ml larutan Fe (II) 0,05 M dan 20 ml
yang larutan H2O2 30%, kemudian didiamkan selama
semalam. Setelah itu, sebanyak 20 ml H2O2 30%
dan 20 ml Fe (II) 0,05 M ditambahkan kembali
ke dalam sampel. Sampel didiamkan selama 5
menit, kemudian diletakkan di atas pemanas
(hotplate) dengan suhu 75°C dan kecepatan
putaran 180 rpm dalam waktu 30 menit. Selama
Tahapan proses pelarutan ini ditambahkan larutan N-
Hexane sebanyak 4 – 6 ml untuk menghilangkan
lemak yang dapat menempel pada alat serta
mengikat sampel yang dapat mengganggu
proses selanjutnya.
Penyaringan sampel
Pada tahap penyaringan sampel dilakukan
sampel, dengan modifikasi metode Masura et al. (2015).
Modifikasi dilakukan dengan menghilangkan
flotasi sampel dan menambahkan proses
penyaringan vakum dengan kain saring 100 µm
yang digunting berbentuk lingkaran menyerupai
kertas saring pada umumnya. Penghilangan
floatasi sampel ditujukan untuk mengeliminir
peluang mikroplastik yang tidak terapung
dikarenakan faktor berat jenis maupun terikat
dengan bahan organik pada proses sebelumnya.
Pada tahapan ini, cawan petri yang sudah diberi
label disiapkan untuk wadah kain saring setelah
proses penyaringan. Hasil saringan didiamkan
selama semalam pada suhu ruang sampai
mengering.

Tahap perlakuan hidrogen peroksida Identifikasi mikroplastik

Tahap perlakuan hidrogen peroksida Identifikasi mikroplastik dibagi menjadi
dilakukan dengan menggunakan metode Masura tiga tahap, yaitu identifikasi menggunakan
et al. (2015) yang dimodifikasi dengan penyinaran lampu, identifikasi dengan
dilakukan dengan menggunakan metode Masura mikroskop, serta uji FTIR. Langkah pertama

4 Jurnal Iktiologi Indonesia

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