AEROBIC GRAM POSITIVE BACILLI

Corynebacterium Listeria Erysipelothrix Lactobacillus Streptomycetes Nocardia

diphtheriae monocytogenes rhusopathiae spp. spp. spp.
Bacilli swelling at one or Facultative intracellularly Animal pathogen Uses sugar for Chains of spores Forms branching
both end (babes-earnts located Most common in energy at maturity filaments
Description granules/ volutin) Actin filaments to move inside swines Lactic acid by- Helps in Weak acid fast
Club shaped host cell Resistant to products decomposition of Pleomorphic
X,V, Y arrangement “Tumbling motility” at 25c smoking and Used as probiotic organic matter in Beaded
Endotoxin(+) pickling process, Non motile soil appearance
Catalase(+) dessication Facultative Used for broad
Produce narrow zone of b- Short chain pattern anaerobe spectrum
hemolysis antibiotics
Can tolerate cold (2-4c) production
Colonizes GI and female GUT
Exotoxin (lysogenic property) D-galactose residues Hyaluronidase
Virulence 1. subunit a: ADP- Internalin Neuramidase
Factor ribosylation activity Listeriolysin O (LLO)
2. subunit b: binds to Actin A
cell surface Phospholipase subs.
Catalase
Superoxide dismutase
Potassium tellurite plate: Small, round, smooth BAP (5-10% CO2): MRS media: small Buffered charcoal
Culture grayish black colonies transluscent a-hemolysis white creamy yeast extract:
BAP Narrow zone of b-hemolysis colonies bread crumbs
SBA, CHOC, BHI, Thioglycollate colony
Cutaneous: deep erosive Bacteremia Erysipeloid: Mycetoma
Pathogenesis non-healing ulcer covered Meningitis, inflammation of
with gray-brown membrane meningoencephalitis traumatized skin
Respiratory: bull neck, Septicemia Erysipelas
pseudomembrane Placental abscess, spontaneous
abortion
Respiratory droplets Ingestion of contaminated food Direct contact with Penetration of Inhalation
Transmission infected meat wound or skin Traumatic
abrasion implantation
mycetoma
Specimen: nasopharynx or End-over-end tumbling motility Specimen: PCR PCR
Lab Dx throat swab CAMP: positive aspirate/biopsy Culture Culture
Loeffler’s slant: for volutin Hippurate Hydrolysis: positive from margin of Direct microscopy Direct microscopy
granules Bile Esculin: positive lesion
Elek’s toxigenicity test