CHEMISTRY 59-321.

Principles of Instrumental Analysis.

The fundamental principles of operation and practical application of modern analytical
instrumentation are presented. Acquisition of qualitative and quantitative chemical,
biochemical and bioanalytical data from these instruments and methods describes the
atomic and molecular composition and structure of matter. Topics covered in this course
include separation methods such as gas and liquid chromatography and capillary
electrophoresis atomic and molecular absorption and emission spectroscopy and surface
techniques. (Prerequisite: 59-320)
LECTURES: Tuesday and Thursday at 8:30 -9:50 am.
Place: TC 204
Midterm: March 3 at 8:30 am
Final Exam. April 14 at 8:30 am (Thursday)

INSTRUCTOR: • Professor Ricardo Aroca

• g57@uwindsor.ca

OFFICE I am virtually available via email for your questions; I check my email
HOURS: regularly every day (even on weekends), so you should be able to get
an email reply to a question within 24 hours of your posting it to me
(barring any unforeseen technical difficulties!).

GRADING: There will be one midterm exam and one comprehensive Final Exam.
Your grade for the course will be determined as follows:

Midterm 25%
Laboratory 25%
Final
50%
Exam:

TOTAL: 100 %
LECTURE: I encourage you to come to lecture having already read the assigned
material as I would prefer to spend as much of our class time discussing
the material, answering your questions about the material and asking you
questions about the material. Obviously, our time in class will only be
meaningful if you've already read the assigned readings for that particular
unit. Your attendance in class is, of course, expected.
TEXT: Principles of Instrumental Analysis - 6th Edition by Douglas A. Skoog,
F. James Holler, and Stanley R. Crouch. Thomson Brook/Cole, 2007

Chapters included for the semester.

1. Chapter 1. Introduction
2. Chapter 5. Signal and Noise.
Section V: Separation Methods.
3. Chapter 26. An Introduction to Chromatographic Separations.
4. Chapter 27. Gas Chromatography.
5. Chapter 28. Liquid Chromatography.
Section II: Atomic Spectroscopy.
6. Chapter 6. An Introduction to Spectrometric Methods.
7. Chapter 7. Components of Optical Instruments.
8. Chapter 8. An Introduction to Optical Atomic Spectrometry.
9. Chapter 9. Atomic Absorption and Atomic Fluorescence Spectrometry.
Section III: Molecular Spectroscopy.
10. Chapter 13. An Introduction to Ultraviolet/Visible Molecular Absorption
Spectrometry.
11. Chapter 14. Applications of Ultraviolet/Visible Molecular Absorption
Spectrometry.
12. Chapter 15. Molecular Luminescence Spectrometry.
13. Chapter 16. An Introduction to Infrared Spectrometry.
14. Chapter 17. Applications of Infrared Spectrometry.
15. Chapter 18. Raman Spectroscopy.
16. Chapter 21. Surface Characterization by Spectroscopy and Microscopy
Appendix one: Evaluation of analytical data.