CLPC 26

:BIOCHEMICAL
ENGINEERING
D R . M . A R I VA Z H A G A N
P RO F E S S O R
D E PA RT M E N T O F
CHEMICAL
ENGINEERING
LECTURE #1
2 7- 0 8 - 2 0 2 0
 Introduction to Bioscience: Types of Microorganisms: Structure
and function of microbial cells.

Fundamentals of microbial growth, batch and continuous culture.

Isolation and purification of Enzymes from cells. Cell Growth
Measurement.

Syllabus Functioning of Cells and Fundamental Molecular Biology:

Metabolism and bio-energetics, Photosynthesis, carbon
metabolism,

EMP pathway, tricarboxylic cycle and electron transport chain,

aerobic and anaerobic metabolic pathways.

Synthesis and regulation of biomolecules, fundamentals of

microbial genetics, role of RNA and DNA.
 Enzyme kinetics: Simple enzyme kinetics, Enzyme reactor with
simple kinetics. Inhibition of enzyme reactions. Other influences on
enzyme activity. Immobilization of enzymes.

Effect of mass transfer in immobilized enzyme particle systems.

Industrial applications of enzymes.

Cell kinetics and fermenter design: Growth cycle for batch

cultivation, Stirred-tank fermenter,

Multiple fermenters connected in series. Cell recycling. Structured

Syllabus Model.

Introduction to Bioreactor design: Continuously Stirred aerated

tank bioreactors.

Mixing power correlation. Determination of volumetric mass

transfer rate of oxygen from air bubbles and effect of mechanical
mixing and aeration on oxygen transfer rate, heat transfer and
power consumption.

Multiphase bioreactors and their applications. Downstream

processing and product recovery in bioprocesses.
 1. J. E. Bailey and D. F. Ollis. " Biochemical
Engineering Fundamentals", 2nd Edn.,
McGraw Hill, New York , 1986.

2. Trevan, Boffey, Goulding and Stanbury, "

Biotechnology", Tata McGraw Hill
Reference Publishing Co., New Delhi, 1987.

Books 3. H. W. Blanch and D. S. Clark,

“Biochemical Engineering”, Marcel
Dekker, Inc., New York, 1996.

4. M. L. Shuler and F. Kargi, “Bio Process

Engineering: Basic concepts”, 2ndEdn.,
Prentice Hall of India, New Delhi, 2002.
Relationship between Biological Science ,
Process and Chemical Engineering

Biological Sciences Process Chemical

Engineering
Systematics Culture choice Reactor design

Genetics Mass culture

Process control
Biochemistry Cell response
Unit operation
Physiology Process operation
Energy and Material
Chemistry Product recovery Utilization
 Problem1
In the Processing of a
particular Bioprocess
five purification steps
are employed each
giving a 90% yield of
active bioproducts
what will be the
overall yield?
 Problem1
In the Processing of a Answer:
particular Bioprocess = 100x(0.9x0.9x0.9x0.9x0.9)
five purification steps
are employed each = 100x (0.9)
giving a 90% yield of
active bioproducts = 59%
what will be the
overall yield?
 Problem2
Plot a graph of percentage
overall yield against umber
of steps ( upto 8 steps) a) The step yield is 90% ?
when, for each step yield is
80%. Similarly plot graphs b) The step yield is 85% ?
for step yield of 85, 90 and c) The step yield is 80% ?
95%. If for an industrial
process, we need an overall
yield of 60% how many
process steps can we
afford to carry out if:
 Answer:
Problem2
Plot a graph of percentage
overall yield against umber
of steps ( upto 8 steps)
when, for each step yield is

Overall yield (%)

80%. Similarly plot graphs
for step yield of 85, 90 and
95%. If for an industrial
process, we need an overall
yield of 60% how many
process steps can we
afford to carry out if:
Thank you