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2016 Life Science Biorad
2016 Life Science Biorad
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Overview
Life Science Research 2016
New Products ii
Sample Preparation 1
Sample Quantitation 19
Cell Counting 29
Flow Cytometry 33
Cell Imaging 47
Antibodies 275
Transfection 335
Note: Due to the nature of new products, some items in this catalog may not be available for immediate purchase. For more information, contact your local Bio-Rad sales representative.
New Products
bio-rad.com
New Products
Antibodies
PrecisionAb™ Validated Western Blotting Antibodies
Rigorous validation using 12 different whole-cell lysates expressing endogenous
levels of the target proteins makes the PrecisionAb line of primary antibodies
optimally suited for generating dependable data in western blotting applications.
With stringent quality control, trial-size availability for assessing performance,
inclusion of a positive control lysate that allows benchmarking target bands for
identification, complete validation data, and detailed protocols, the PrecisionAb
line of antibodies provide high specificity and sensitivity and offer overall ease
of use with minimal trial and error. Page 277.
DNA Amplification/PCR
Droplet Digital™ PCR System Reagents
ddPCR™ Residual DNA Quantification Supermix and Kits
ddPCR residual DNA quantification supermix and kits enable precise quantification
of low levels of Chinese hamster ovary (CHO), Escherichia coli, yeast, human, and
mouse DNA for residual host cell DNA monitoring. DNA can be quantified directly
from various sample matrices without DNA purification. Page 352.
Biotechnology Explorer™
Photosynthesis and Cellular Respiration Kit
This ThiNQ!™ investigation kit facilitates inquiry-based learning and
teaches photosynthesis and cellular respiration concepts using easy-to-
handle algae beads and a simple colorimetric assay. The kit is aligned
with AP Biology learning objectives and it encourages and supports
inquiry in its teacher and student manuals. Page 418.
Chromatography
ChromLab™ Software, User Management Edition
The User Management Edition of ChromLab software enables multiple
system control from a single PC and the ability to save all methods,
runs, and analyses to a central database. Data can be shared among
ChromLab computers and NGC™ chromatography systems.
Page 121.
Ordering Information
Catalog # Description Price, U.S. $
1614011 SureBeads Protein A Magnetic Beads, 1 x 1 ml vial conjugated magnetic immunoprecipitation beads 83.00
(10 mg beads/ml suspension)
1614013 SureBeads Protein A Magnetic Beads, 1 x 3 ml vial conjugated magnetic immunoprecipitation beads 160.00
(10 mg beads/ml suspension)
1614021 SureBeads Protein G Magnetic Beads, 1 x 1 ml vial conjugated magnetic immunoprecipitation beads 83.00
(10 mg beads/ml suspension)
1614023 SureBeads Protein G Magnetic Beads, 1 x 3 ml vial conjugated magnetic immunoprecipitation beads 160.00
(10 mg beads/ml suspension)
1614916 16-Tube SureBeads Magnetic Rack, with separable magnets 345.00
1614811 SureBeads Trial Kit Protein A, 1 x 1 ml vial conjugated magnetic immunoprecipitation beads 73.00
(10 mg beads/ml suspension), 4-tube magnetic rack
1614821 SureBeads Trial Kit Protein G, 1 x 1 ml vial conjugated magnetic immunoprecipitation beads 73.00
(10 mg beads/ml suspension), 4-tube magnetic rack
1614833 SureBeads Starter Kit Protein A and G, 1 x 3 ml vial protein A and 1 x 3 ml vial protein G conjugated magnetic 585.00
immunoprecipitation beads (10 mg beads/ml suspension), 16-tube magnetic rack
1614813 SureBeads Starter Kit Protein A, 2 x 3 ml vials protein A conjugated magnetic immunoprecipitation beads 585.00
(10 mg beads/ml suspension), 16-tube magnetic rack
1614823 SureBeads Starter Kit Protein G, 2 x 3 ml vials protein G conjugated magnetic immunoprecipitation beads 585.00
(10 mg beads/ml suspension), 16-tube magnetic rack
Protein Extraction
Protein extraction tools such as cell lysis and extraction kits, as well as mini grinders, are available for extracting proteins
from cultured cells and tissues.
Ordering Information
Catalog # Description Price, U.S. $
1632086 ReadyPrep Protein Extraction Kit (Total Protein), 20 preps 190.00
Ordering Information
Catalog # Description Price, U.S. $
1632141 Mammalian Cell Lysis Kit, 15 preps, includes 50 ml protein solubilization buffer (PSB), ReadyPrep mini 275.00
grinders (2 packs of 10 each)
1632142 Plant Cell Lysis Kit, 10 preps, includes 50 ml protein solubilization buffer (PSB), ReadyPrep 2-D cleanup kit 350.00
(50 reaction size)
1632143 Yeast Cell Lysis Kit, 15 preps, includes 50 ml protein solubilization buffer (PSB), 15 ml yeast suspension 300.00
buffer, 2 x 0.5 ml lyticase (1.5 U/µl)
1632144 Bacterial Cell Lysis Kit, 15 preps, includes 50 ml protein solubilization buffer (PSB), 25 ml bacteria 300.00
suspension buffer, 1 ml lysozyme (1,500 U/µl)
Cell Lysis Kit Components
1632145 Protein Solubilization Buffer (PSB), makes 50 ml of solution 125.00
1632146 ReadyPrep Mini Grinders, includes 20 mini grinders, sufficient for twenty 100 mg extractions 125.00
Bio-Plex Cell Lysis Kits
171304011 Bio-Plex Cell Lysis Kit, 1 x 96-well, includes cell lysis and wash buffers, factor 1 and factor 2 180.00
171304012 Bio-Plex Cell Lysis Kit, 10 x 96-well, includes cell lysis and wash buffers, factor 1 and factor 2 1,510.00
See Also
Ordering Information
Bio-Spin gel filtration
Catalog # Description Price, U.S. $
columns: page 16.
ReadyPrep Kits Micro Bio-Spin
1632130 ReadyPrep 2-D Cleanup Kit, 50 preps 315.00 gel filtration columns:
1632140 ReadyPrep 2-D Cleanup Kit, 5 preps 125.00 page 16.
1632105 ReadyPrep 2-D Starter Kit, includes protein sample and reagents sufficient to rehydrate, focus, and transfer 260.00
to second-dimension gels; six 17 cm, ten 11 cm, or sixteen 7 cm ReadyStrip IPG strips (ReadyStrip IPG
strips*, precast SDS-PAGE gels, and gel stains not included in kit)
1632090 ReadyPrep Reduction-Alkylation Kit, 100 preps 215.00
ReadyPrep Kit Components and Related Products
1632091 ReadyPrep Proteomics Grade Water, 500 ml 40.00
1632083 ReadyPrep 2-D Rehydration/Sample Buffer 1, 10 ml, 7 M urea, 2 M thiourea, 1% ASB-14, 40 mM Tris, 38.00
0.001% bromophenol blue
1632106 ReadyPrep 2-D Starter Kit Rehydration/Sample Buffer, 10 ml, 8 M urea, 2% CHAPS, 50 mM DTT, 0.2% 44.00
Bio-Lyte 3/10 ampholyte, 0.001% bromophenol blue
Reducing and Alkylating Agents
1610611 Dithiothreitol (DTT), 5 g 81.00
1632101 Tributylphosphine (TBP), 200 mM, 0.6 ml 44.00
1632109 Iodoacetamide, 30 g 145.00
* See the prepacked Bio-Spin and Micro Bio-Spin gel filtration columns on pages 16–17.
Protein Fractionation
Fractionation kits reduce sample complexity, helping to identify low-abundance proteins. Fractionation kits can be
subdivided into three groups that fractionate based on differential solubility, cellular location of the proteins of interest,
and protein charge.
Fractionation by Solubility
Fractionation Using Differential Protein Solubility visualization of proteins that might not otherwise be seen.
The ReadyPrep sequential extraction kit and the ReadyPrep Increasing solubilization strength is provided through the
protein extraction kit (soluble/insoluble) both reduce sample use of stronger detergents for each subsequent fraction
complexity using differential solubilization. The two kits ■■
eadyPrep protein extraction kit (soluble/insoluble) —
R
can be used independently, or the rehydration/sample uses a single fractionation step
buffer from the soluble/insoluble kit can be used with the
sequential extraction kit to create a fourth fraction for even ReadyPrep Kit Components and Related Products
better resolution. Individual ReadyPrep kit components and related products
such as reducing agents are also available. See ordering
■■
eadyPrep sequential extraction kit — enables the
R
information on pages 4–5.
isolation of 3 different fractions of increasing solubility.
These fractions are isolated sequentially, allowing the For More Information
Web: bio-rad.com/proteinfractionation
Request or download bulletins: 2934 and 2961
Ordering Information
Catalog # Description Price, U.S. $
ReadyPrep Kits
1632100 ReadyPrep Sequential Extraction Kit, 15 preps 215.00
1632085 ReadyPrep Protein Extraction Kit (Soluble/Insoluble), 20 preps 235.00
ReadyPrep Kit Components and Related Products
1632102 ReadyPrep Sequential Extraction Kit Reagent 1, 50 ml, 40 mM Tris base 59.00
1632103 ReadyPrep Sequential Extraction Kit Reagent 2, 10 ml, 8 M urea, 4% CHAPS, 40 mM Tris 36.00
base, 0.2% Bio-Lyte 3/10 ampholyte
1632104 ReadyPrep Sequential Extraction Kit Reagent 3, 10 ml, 5 M urea, 2 M thiourea, 2% CHAPS, 2% SB 3–10, 36.00
40 mM Tris base, 0.2% Bio-Lyte 3/10 ampholyte
1632083 ReadyPrep 2-D Rehydration/Sample Buffer 1 38.00
Ordering Information
Catalog # Description Price, U.S. $
ReadyPrep Kits
1632089 ReadyPrep Protein Extraction Kit (Cytoplasmic/Nuclear), 50 preps 450.00
1632088 ReadyPrep Protein Extraction Kit (Membrane I), 50 preps 450.00
1632084 ReadyPrep Protein Extraction Kit (Membrane II), 10 preps 270.00
1632087 ReadyPrep Protein Extraction Kit (Signal), 50 preps 230.00
Related Products
1632083 ReadyPrep 2-D Rehydration/Sample Buffer 1 38.00
1632085 ReadyPrep Protein Extraction Kit (Soluble/Insoluble), 20 preps 235.00
Fractionation by Charge
Ordering Information
Catalog # Description Price, U.S. $
7326703 Aurum CEX Mini Columns, 25 pack 390.00
7326706 Aurum AEX Mini Columns, 25 pack 390.00
Protein Depletion
Complex samples often require depletion of high-abundance proteins to allow the detection of the low-abundance ones.
Bio-Rad offers two different methodologies for protein depletion — the ProteoMiner™ protein enrichment system
(which utilizes hexapeptide libraries) and Aurum™ serum and Affi-Gel® Blue products (which utilize resins).
The ProteoMiner protein enrichment system is a novel ProteoMiner Protein Enrichment Kits — these kits
sample preparation tool for reducing the dynamic range can be used with a variety of biological samples and
of protein concentrations in complex biological samples. are compatible with all major downstream proteomics
The ProteoMiner system: applications. Small- and large-capacity kits are available
■■
Enriches and concentrates low-abundance proteins that for processing two or ten samples.
cannot be detected through traditional methods ProteoMiner Sequential Elution Kits — these kits utilize
■■
orks with a variety of sample types (serum, plasma, urine,
W multiple elution reagents to sequentially elute proteins
bile, cell lysates, tissues, and platelets) and is not limited by based on different properties.
the species’ origin
■■
tilizes a combinatorial library of hexapeptides rather
U
than immunodepletion, minimizing dependence on
available antibodies and preventing the codepletion of
low-abundance proteins
Proteins
ProteoMiner
beads
ProteoMiner technology is based on the interaction of complex protein samples with a large, highly diverse library of hexapeptides bound
to chromatographic supports. In theory, each unique hexapeptide binds to a unique protein sequence. Because the bead capacity limits binding
capacity, high-abundance proteins quickly saturate their ligands (red and yellow beads) and excess protein is washed out during the procedure. In
contrast, low-abundance proteins are concentrated on their specific ligands (pink and teal beads), thereby decreasing the dynamic range of proteins in
the sample. When analyzed in downstream applications, the number of proteins detected is dramatically increased.
Ordering Information
Catalog # Description Price, U.S. $
1633006 ProteoMiner Protein Enrichment Small-Capacity Kit, 10 preps, for processing 10 mg of total protein, 665.00
includes 10 spin columns, wash buffer, elution reagents, collection tubes
1633007 ProteoMiner Protein Enrichment Large-Capacity Kit, 10 preps, for processing 50 mg of total protein, 910.00
includes 10 spin columns, wash buffer, elution reagents, collection tubes
1633008 ProteoMiner Protein Enrichment Introductory Small-Capacity Kit, 2 preps, for processing 10 mg of total 215.00
protein, includes 2 spin columns, wash buffer, elution reagents, collection tubes
1633009 ProteoMiner Protein Enrichment Introductory Large-Capacity Kit, 2 preps, for processing 50 mg of total 245.00
protein, includes 2 spin columns, wash buffer, elution reagents, collection tubes
ProteoMiner Sequential Elution Kits
1633010 ProteoMiner Sequential Elution Small-Capacity Kit, 10 preps, for processing 10 mg of total protein, 805.00
includes 10 spin columns, wash buffer, 4 sequential elution reagents, collection tubes
1633011 ProteoMiner Sequential Elution Large-Capacity Kit, 10 preps, for processing 50 mg of total protein, 1,050.00
includes 10 spin columns, wash buffer, 4 sequential elution reagents, collection tubes
ProteoMiner Kit Accessories
1633003 ProteoMiner Sequential Elution Reagents, 10 preps, includes reagents only (columns not included), 300.00
to be used with #1633006 or #1633007
The Aurum™ Affi-Gel® Blue and Aurum serum kits and Before After
columns use affinity chromatography to reduce albumin and
IgG, which improves analysis of lower abundance proteins.
Heavy- Heavy-
These products utilize a quick and easy spin-column format chain chain
Albumin Albumin
and provide eluted proteins ready for analysis. IgG IgG
Light- Light-
For More Information chain chain
Web: bio-rad.com/proteindepletion IgG IgG
Request or download bulletin: 2823
Removal of albumin and IgG from serum using the Aurum serum
protein mini kit. Total protein (1.32 mg) was purified on an Aurum
serum protein mini column. Left, untreated serum; right, serum treated
with the Aurum kit.
Ordering Information
Catalog # Description Price, U.S. $
7326701 Aurum Serum Protein Mini Kit, 10 pack, includes columns and buffers 525.00
7326708 Aurum Affi-Gel Blue Mini Columns, 25 pack 390.00
RNA Isolation
Bio-Rad offers a broad range of sample preparation products for RNA isolation. Scalable and high-throughput products
are available.
Purity A260 /A280 of 1.9–2.1 A260 /A280 of 1.9–2.1 A 260 /A 280 of 1.9–2.1 A 260 /A 280 of 1.9–2.1
Ordering Information
Catalog # Description Price, U.S. $
7326820 Aurum Total RNA Mini Kit, 50 preps, includes 50 RNA binding columns, 50 capless collection tubes 230.00
(2.0 ml), 100 capped sample tubes (2.0 ml), 50 capped sample tubes (1.5 ml), 1 vial lyophilized DNase I,
RNase-free reagents
7326830 Aurum Total RNA Fatty and Fibrous Tissue Kit, 50 preps, includes #7326870, 50 ml PureZOL RNA 355.00
isolation reagent
7326870* Aurum Total RNA Fatty and Fibrous Tissue Module, 50 preps, includes 50 RNA binding columns, 250.00
50 capless collection tubes (2.0 ml), 100 capped sample tubes (2.0 ml), 50 capped sample tubes (1.5 ml),
1 vial lyophilized DNase I, RNase-free reagents, and plasticware
7326800 Aurum Total RNA 96 Kit, 2 x 96-well preps, includes 2 grow blocks, sealing tape, 2 RNA binding plates, 580.00
2 collection microplates, 2 vials lyophilized DNase I, RNase-free reagents
Aurum Consumables and Accessories
7326828 DNase I, RNase-free, lyophilized, 1 vial 57.00
7326801 Aurum Total RNA Elution Solution, RNase-free, 20 ml 16.00
7326802 Aurum Total RNA Lysis Solution, RNase-free, 85 ml 52.00
7326803 Aurum Total RNA Wash High-Stringency Solution, RNase-free, 150 ml 40.00
7326804 Aurum Total RNA Wash Low-Stringency Solution, 60 ml 31.00
7326805 Aurum DNase Dilution Solution, 20 ml 16.00
7326826 Aurum RNA Binding Mini Columns, 50 30.00
* Does not include PureZOL RNA isolation reagent (see catalog #7326880 or #7326890 to order separately).
Ordering Information
Catalog # Description Price, U.S. $
7326880 PureZOL RNA Isolation Reagent, 50 ml 110.00
7326890 PureZOL RNA Isolation Reagent, 100 ml 190.00
RFU
purify RNA. Reverse transcription and real-time PCR (qPCR)
can be performed directly from cell lysates. This reagent is 102
ideal for rapid, high-throughput gene expression analysis
such as validation of siRNA-mediated gene knockdown.
101
■■
Rapid protocol (5–10 min) efficiently stabilizes RNA
0 10 20 30 40
■■
ensitive detection of high-, medium-, and low-copy
S Cycles
number gene targets directly from cell lysates iScript RT-qPCR sample preparation reagent provides rapid
■■
eagent enables multiplex qPCR detection of up to 4
R yet sensitive gene expression results for high-, medium-, and
low-copy number gene targets. HeLa cells were treated with
targets from as few as 10 cells iScript RT-qPCR sample preparation reagent at 125 cells/μl and the
For More Information expression levels of three different genes were assessed. 18S rRNA
Web: bio-rad.com/iscript-sampleprep (—), b-tubulin (—), and CMYC (—) expression levels were determined by
Request or download bulletin: 5736 performing reverse transcription (iScript cDNA synthesis kit) and qPCR
(iQ™ SYBR® Green supermix) directly from cell lysate preparations.
RFU, relative fluorescence units.
Cells Lysis
Bind to
DNase Elute for Standard method:
column >30 min
treatment RT-qPCR >30 min
matrix
Ordering Information
Catalog # Description Price, U.S. $
1708898 iScript RT-qPCR Sample Preparation Reagent, 100 reactions, 10 ml, contains RNase inhibitors and 150.00
RNA stabilizers
1708899 iScript RT-qPCR Sample Preparation Reagent, 500 reactions, 5 x 10 ml, contains RNase inhibitors and 530.00
RNA stabilizers
DNA Isolation
DNA Isolation Kit Selection Guide
DNA Preparation Bacterial Culture Purification Binding Growth
Kit Yield Time Volume Format Matrix Media
Quantum Prep® ≤40 µg <15 min 1–10 ml Spin Diatomaceous Enriched
plasmid miniprep earth or standard
Quantum Prep ≤400 µg 45 min 20–40 ml Spin Diatomaceous Enriched
plasmid midiprep earth or standard
Aurum™ plasmid ≤20 µg <10 min 1–5 ml Vacuum* Silica Standard
mini and spin membrane
* Using the Aurum vacuum manifold. This product is no longer available. Please contact Bio-Rad Technical Support for information on
alternative products.
Ordering Information
Catalog # Description Price, U.S. $
7326100 uantum Prep Plasmid Miniprep Kit, 100 preps, includes 25 ml cell resuspension solution, 25 ml cell lysis
Q 195.00
solution, 25 ml neutralization solution, 20 ml Quantum Prep matrix, 63 ml wash solution, 100 spin columns
7326120 Quantum Prep Plasmid Midiprep Kit, 20 preps, includes 110 ml cell resuspension solution, 110 ml cell lysis 190.00
solution, 110 ml neutralization solution, 20 ml Quantum Prep matrix, 125 ml wash solution, 20 midi spin columns
Ordering Information
Catalog # Description Price, U.S. $
7326400 Aurum Plasmid Mini Kit, 100 preps, includes plasmid-binding mini columns, 100 capless collection tubes, 217.40
reagents, protocol overview
InstaGene Protocol
PCR-ready
Add bacterial colony to water. Spin and remove supernatant. Add InstaGene matrix and incubate at 56°C for 30 min. Spin; use supernatant for PCR.
Vortex and incubate at 100°C for 8 min.
Ordering Information
Catalog # Description Price, U.S. $
7326030 InstaGene Matrix, 20 ml, sufficient for 100 extractions 54.00
Ordering Information
Catalog # Description Price, U.S. $
1421253 Chelex 100 Resin, molecular biology grade, 50 g, sodium, 200–400 dry mesh, 75–150 µm wet bead 195.00
DNA Cleanup
Bio-Rad offers a variety of sample preparation and cleanup columns; many are also suitable for DNA fragment purification.
Purified DNA
Cut out gel slice. Chop into pieces. Transfer to spin column Spin for 3 min. Recover purified DNA.
and freeze for 5 min.
Ordering Information
Catalog # Description Price, U.S. $
7326165 reeze ’N Squeeze DNA Gel Extraction Spin Columns, 25 columns
F 60.00
7326166 Freeze ’N Squeeze DNA Gel Extraction Spin Columns, 100 columns 185.00
Prepacked size exclusion spin columns allow easy These columns are available in multiple sizes and offer
cleanup and purification of DNA and proteins from lower multiple MW exclusion limits to accommodate a variety
MW contaminants. Bio-Spin®, Micro Bio-Spin™, and of needs. Use the chart below to choose the column that
PCR Kleen™ columns clean up DNA or protein samples best meets your needs.
quickly and easily using size exclusion chromatography.
PCR Kleen columns are prepacked spin columns for Luer end fitting
purifying PCR products and other DNA molecules >200 bp with snap-off tip
directly from reaction mixtures. A simple 4-minute spin Prepacked Bio-Spin and Micro Bio-Spin Columns
effectively removes salts, nucleotides, enzymes, primers,
and primer-dimers. Purified DNA fragments are immediately
available for secondary PCR, subcloning, restriction digests,
ligations, sequencing, and other enzymatic manipulations.
For More Information
Web: bio-rad.com/dna-cleanup PCR Kleen Spin Columns
Request or download bulletin: 2311
Ordering Information
Catalog # Description Price, U.S. $
Micro Bio-Spin Columns with Bio-Gel P-6 in Tris Buffer
7326221 Micro Bio-Spin 6 Columns, includes 25 columns in Tris buffer, 50 collection tubes 110.00
7326222 Micro Bio-Spin 6 Columns, includes 100 columns in Tris buffer, 200 collection tubes 365.00
Micro Bio-Spin Columns with Bio-Gel P-30 in Tris Buffer
7326223 Micro Bio-Spin 30 Columns, includes 25 columns in Tris buffer, 50 collection tubes 110.00
7326224 Micro Bio-Spin 30 Columns, includes 100 columns in Tris buffer, 200 collection tubes 365.00
7326250 Micro Bio-Spin 30 Columns, includes 25 columns in Tris buffer, 50 collection tubes, RNase-free 125.00
7326251 Micro Bio-Spin 30 Columns, includes 100 columns in Tris buffer, 200 collection tubes, RNase-free 440.00
Micro Bio-Spin Columns with Bio-Gel P-6 in SSC Buffer
7326200 Micro Bio-Spin 6 Columns, includes 25 columns in SSC buffer, 50 collection tubes 110.00
7326201 Micro Bio-Spin 6 Columns, includes 100 columns in SSC buffer, 200 collection tubes 365.00
Micro Bio-Spin Columns with Bio-Gel P-30 in SSC Buffer
7326202 Micro Bio-Spin 30 Columns, includes 25 columns in SSC buffer, 50 collection tubes 110.00
7326203 Micro Bio-Spin 30 Columns, includes 100 columns in SSC buffer, 200 collection tubes 365.00
Bio-Spin Columns with Bio-Gel P-6 in Tris Buffer
7326227 Bio-Spin 6 Columns, includes 25 columns in Tris buffer, 50 collection tubes 135.00
7326228 Bio-Spin 6 Columns, includes 100 columns in Tris buffer, 200 collection tubes 455.00
Bio-Spin Columns with Bio-Gel P-30 in Tris Buffer
7326231 Bio-Spin 30 Columns, includes 25 columns in Tris buffer, 50 collection tubes 135.00
7326232 Bio-Spin 30 Columns, includes 100 columns in Tris buffer, 200 collection tubes 455.00
Bio-Spin Columns with Bio-Gel P-6 in SSC Buffer
7326002 Bio-Spin 6 Columns, includes 25 columns in SSC buffer, 50 collection tubes 155.00
Bio-Spin Columns with Bio-Gel P-30 in SSC Buffer
7326006 Bio-Spin 30 Columns, includes 25 columns in SSC buffer, 50 collection tubes 155.00
PCR Kleen Spin Columns
7326300 PCR Kleen Spin Columns, 25 78.75
Subject Index
Aurum™ Kits and Columns, 8
Aurum™ Plasmid Mini Kit, 14
Aurum™ Total RNA Kits, 10
Chelex® 100 Molecular Biology Grade Resin, 15
Fractionation by Cellular Location, 6
Fractionation by Charge, 6
Fractionation by Solubility, 5
Freeze ’N Squeeze™ DNA Gel Extraction Spin Columns, 15
InstaGene™ Matrix, 14
iScript™ RT-qPCR Sample Preparation Reagent, 12
Kits for Cell Lysis, 3
Prepacked Spin Columns, 16
ProteoMiner™ Protein Enrichment System, 7
PureZOL™ RNA Isolation Reagent, 11
Quantum Prep® Plasmid Kits, 13
ReadyPrep™ Protein Extraction Kit, 3
SureBeads™ Magnetic Beads System, 2
Spectrophotometry 23
Cuvettes 25
Protein Assay Kits
bio-rad.com/proteinassays
Ordering Information
Catalog # Description Price, U.S. $
5000201 uick Start Bradford Protein Assay Kit 1, includes 1x dye reagent (1 L), bovine serum albumin standard
Q 150.00
(5 x 2 mg/ml); sufficient for 200 standard assays or 4,000 microplate assays
5000202 Quick Start Bradford Protein Assay Kit 2, includes 1x dye reagent (1 L), bovine serum albumin standard set 190.00
(2 sets of 7 concentration standards, 0.125–2.0 mg/ml, 2 ml)
5000203 Quick Start Bradford Protein Assay Kit 3, includes 1x dye reagent (1 L), bovine g-globulin standard 155.00
(5 x 2 mg/ml)
5000204 Quick Start Bradford Protein Assay Kit 4, includes 1x dye reagent (1 L), bovine g-globulin standard set 195.00
(2 sets of 7 concentration standards, 0.125–2.0 mg/ml, 2 ml)
Reagents
5000205 Quick Start Bradford 1x Dye Reagent, 1 L 115.00
Accessories
5000206 Quick Start Bovine Serum Albumin Standard, 5 x 2 ml vials of 2 mg/ml 79.00
5000207 Quick Start Bovine Serum Albumin Standard Set, 2 sets of 7 concentration standards, 0.125–2.0 mg/ml 140.00
5000208 Quick Start Bovine g-Globulin Standard, 5 x 2 ml vials of 2 mg/ml 81.00
5000209 Quick Start Bovine g-Globulin Standard Set, 2 sets of 7 concentration standards, 0.125–2.0 mg/ml 140.00
Ordering Information
Catalog # Description Price, U.S. $
5000001 io-Rad Protein Assay Kit I, includes 450 ml dye reagent concentrate, bovine g-globulin standard;
B 150.00
sufficient for 440 standard assays or 2,200 microplate assays
5000002 Bio-Rad Protein Assay Kit II, includes 450 ml dye reagent concentrate, bovine serum albumin standard; 150.00
sufficient for 440 standard assays or 2,200 microplate assays
Reagents
5000006 Bio-Rad Protein Assay Dye Reagent Concentrate, 450 ml 120.00
Accessories
5000005 Protein Standard I, bovine g-globulin, reconstituted volume 20 ml 74.00
5000007 Protein Standard II, bovine serum albumin, reconstituted volume 20 ml 74.00
Ordering Information
Catalog # Description Price, U.S. $
5000111 DC Protein Assay Kit I, includes 250 ml alkaline copper tartrate solution, 2 L dilute Folin reagent, 190.00
5 ml surfactant solution, bovine g-globulin standard; sufficient for 450 standard assays
5000112 DC Protein Assay Kit II, includes 250 ml alkaline copper tartrate solution, 2 L dilute Folin reagent, 175.00
5 ml surfactant solution, bovine serum albumin standard; sufficient for 450 standard assays
Reagents
5000113 Protein Assay Reagent A, 250 ml, alkaline copper tartrate solution 55.00
5000114 Protein Assay Reagent B, 1 L, dilute Folin reagent 55.00
5000115 Protein Assay Reagent S, 5 ml, surfactant solution 37.00
5000116 DC Protein Assay Reagents Package, includes 250 ml alkaline copper tartrate solution, 180.00
2 L dilute Folin reagent, 5 ml surfactant solution; sufficient for 450 standard assays
Accessories
5000005 Protein Standard I, bovine g-globulin, lyophilized (reconstituted volume 20 ml) 74.00
5000007 Protein Standard II, bovine serum albumin, lyophilized (reconstituted volume 20 ml) 74.00
Ordering Information
Catalog # Description Price, U.S. $
5000121 C DC Protein Assay Kit I, includes RC reagents package, DC protein assay reagents package,
R 295.00
bovine g-globulin standard; sufficient for 450 standard assays
5000122 RC DC Protein Assay Kit II, includes RC reagents package, DC protein assay reagents package, 295.00
bovine serum albumin standard; sufficient for 450 standard assays
Reagents
5000113 Protein Assay Reagent A, 250 ml, alkaline copper tartrate solution 55.00
5000114 Protein Assay Reagent B, 1 L, dilute Folin reagent 55.00
5000115 Protein Assay Reagent S, 5 ml, surfactant solution 37.00
5000116 DC Protein Assay Reagents Package, includes 250 ml alkaline copper tartrate solution, 2 L dilute Folin 180.00
reagent, 5 ml surfactant solution; sufficient for 450 standard assays
5000120 RC DC Protein Assay Reagents Package, includes RC reagents package and DC protein assay reagents 290.00
package; sufficient for 450 standard assays
5000119 RC Reagents Package, includes RC reagent I (250 ml) and RC reagent II (250 ml); sufficient for 115.00
500 standard assays
5000117 RC Reagent I, 250 ml 61.00
5000118 RC Reagent II, 250 ml 61.00
Accessories
5000005 Protein Standard I, bovine g-globulin, lyophilized (reconstituted volume 20 ml) 74.00
5000007 Protein Standard II, bovine serum albumin, lyophilized (reconstituted volume 20 ml) 74.00
Spectrophotometry
kits: page 20. solution for the quantitation of dsDNA, ssDNA, or RNA ■■ Xenonflash lamp extends lamp life and reduces
SmartSpec Plus by either using the preprogrammed conversion factors or maintenance costs
spectrophotometer:
page 23. entering a value that is best for the sample being assayed. ■■ Userinterface enables choice of six different languages:
The SmartSpec Plus instrument will provide absorbance,
English, French, German, Italian, Japanese, and Spanish
concentration, and purity values so you can proceed
confidently with downstream experiments. ■■
Compact, space-saving design
Ordering Information
Catalog # Description Price, U.S. $
SmartSpec Plus Spectrophotometer
1702525 SmartSpec Plus Spectrophotometer 5,893.00
Accessories
1702502 Standard Cuvette, 1–3.5 ml, quartz 177.50
1702503 Semimicrovolume Cuvette, 0.5–1.4 ml, quartz 266.25
1702504 Microvolume Cuvette, 200–700 µl, quartz 273.75
1702505 Submicrovolume Cuvette, 80–100 µl, quartz 548.75
1702506 SmartSpec Printer Paper, 5 pack 41.88
1702507 Spring, cuvette holder 25.75
Cuvettes
trUView™ Cuvettes
Ordering Information
Catalog # Description Price, U.S. $
1702510 trUView Cuvettes, pack of 50 72.50
1702511 trUView Cuvettes, pack of 100 138.75
Cuvette Racks
Ordering Information
Catalog # Description Price, U.S. $
Disposable Polystyrene Cuvettes
2239950 Standard Disposable Polystyrene Cuvettes, 3.5 ml, 100 12.50
2239955 Semimicrovolume Disposable Polystyrene Cuvettes, 1.5 ml, 100 12.50
Quartz Cuvettes
1702502 Standard Cuvette, 1–3.5 ml, quartz, for use with the SmartSpec Plus spectrophotometer 177.50
1702503 Semimicrovolume Cuvette, 0.5–1.4 ml, quartz, for use with the SmartSpec Plus spectrophotometer 266.25
1702504 Microvolume Cuvette, 200–700 µl, quartz, for use with the SmartSpec Plus spectrophotometer 273.75
1702505 Submicrovolume Cuvette, 80–100 µl, quartz, for use with the SmartSpec Plus spectrophotometer 548.75
VersaFluor Disposable Cuvettes and Cuvette Racks
1702415 Standard Cuvettes, 12.5 x 12.5 mm (outside dimensions), 4-sided, optically clear polystyrene, 3.5 ml, 100 25.75
1702416 Microcuvettes, 3.0 x 3.0 mm (outside dimensions), 4-sided, optically clear polystyrene, 150–350 µl, 100 46.35
1660485 Cuvette Racks, hold 12 cuvettes each, set of 5 73.75
Subject Index
Bio-Rad Protein Assay, 21
DC™ Protein Assay, 22
Quick Start™ Bradford Protein Assay, 20
RC DC™ Protein Assay, 22
SmartSpec™ Plus Spectrophotometer, 23
Standard and Semimicrovolume Cuvettes, 26
trUView™ Cuvettes, 25
Ordering Information
Catalog # Description Price, U.S. $
TC20 Automated Cell Counter
1450102 TC20 Automated Cell Counter, 120–240 V, includes instrument, power supply, USB flash drive, 4,220.00
30 dual-chamber counting slides (60 counts), 1.5 ml trypan blue
1450103 TC20 Automated Cell Counter with Thermal Label Printer, 120–240 V, includes instrument, 4,750.00
power supply, USB flash drive, USB cable, thermal label printer, 1 roll of 500 labels, 30 dual-chamber
counting slides (60 counts), 1.5 ml trypan blue
Kits and Reagents*
1450003 Counting Kit, includes 30 dual-chamber counting slides (60 counts), 1.5 ml trypan blue 53.00
1450013 Trypan Blue, 0.4% in 0.81% sodium chloride and 0.06% potassium phosphate dibasic solution, 9.00
sterile filtered, sufficient for 150 counts (10 µl/count), 1 x 1.5 ml
1450021 Trypan Blue, 0.4% in 0.81% sodium chloride and 0.06% potassium phosphate dibasic solution, 44.00
sterile filtered, sufficient for 750 counts (10 µl/count), 5 x 1.5 ml
1450022 Trypan Blue, 0.4% in 0.81% sodium chloride and 0.06% potassium phosphate dibasic solution, 82.00
sterile filtered, sufficient for 1,500 counts (10 µl/count), 10 x 1.5 ml
1450015 Counting Slides, 150 dual-chamber counting slides (300 counts) 270.00
1450016 Counting Slides, 300 dual-chamber counting slides (600 counts) 525.00
1450017 Counting Slides, 600 dual-chamber counting slides (1,200 counts) 1,000.00
1450018 Counting Slides, 900 dual-chamber counting slides (1,800 counts) 1,420.00
1450019 Counting Slides, 1,200 dual-chamber counting slides (2,400 counts) 1,760.00
1450020 Counting Slides, 2,400 dual-chamber counting slides (4,800 counts) 3,300.00
1450014 System Test Kit, includes verification slide, instructions 930.00
Accessories*
1450005 Thermal Label Printer, 120–240 V, includes thermal label printer, USB cable, 1 roll of 500 labels 700.00
1450007 Thermal Printer Labels, 1 roll of 500 labels, for thermal label printer 35.00
* Compatible with TC20 and TC10 automated cell counters.
Cell Sorting
Cell sorting provides a method to sort or isolate a homogenous population of cells from a heterogeneous mixture of cells
based on intracellular and/or extracellular properties, typically using fluorescence. Expressed fluorescent proteins, such
as green fluorescent protein (GFP), or fluorophores conjugated to antibodies bind to markers of interest, which allows for a
starting population to be sorted to greater than 99% purity. While cell sorting is commonly used in fields such as immunology,
cancer biology, and stem cell biology, it is quickly becoming a useful tool in broader research studies from microbiology to
neuroscience. Cell sorting can also be used as an upstream tool for proteomic or genomic experiments to achieve more
specific results by reducing background noise or false positives.
Instruments
■■
igh-resolution automated alignment driven
H
by software
Horizontal (X) Focus (Y)
■■
oftware controlled using image processing
S
and algorithms
■■
recise positioning of the nozzle and stream
P
trajectory are fine-tuned with 5-axis piezo-driven
submicron motion control
■■
axes (x, y, and z) for optimal positioning of the
3
nozzle stream at the intersection of the lasers
Roll Pitch
and detectors
■■
axes (roll and pitch) for adjusting the stream
2
trajectory necessary for accurate drop delay
determination and stream alignment
For More Information
■■
eproducible and reliable mechanism with full
R Web: bio-rad.com/cellsorter
nozzle accessibility Request or download bulletins: 6614 and #10032007
Ordering Information
Catalog # Description Price, U.S. $
S3e Cell Sorter
1451005 S3e Cell Sorter (488 nm), 488 nm 100 mW laser, includes 2 fluorescence detectors with filters, Inquire
AutoGimbal system, ProSort software
1451006 S3e Cell Sorter (488/561 nm), 488 nm and 561 nm 100 mW lasers, includes 4 fluorescence Inquire
detectors with filters, AutoGimbal system, ProSort software
1451008 S3e Cell Sorter (488/640 nm), 488 nm and 640 nm 100 mW lasers, includes 4 fluorescence Inquire
detectors with filters, AutoGimbal system, ProSort software
1451078 S3e Biosafety System Class I Inquire
1451029 S3e Cell Sorter (488 nm) with S3e Biosafety System Class I Inquire
1451030 S3e Cell Sorter (488/561 nm) with S3e Biosafety System Class I Inquire
1451032 S3e Cell Sorter (488/640 nm) with S3e Biosafety System Class I Inquire
Accessories
1441001 S3e Sheath Cap Assembly, white 250.00
1441002 S3e DI Water Cap Assembly, blue 250.00
1441003 S3e Waste Cap Assembly, red 250.00
1441005 S3e Filter Block A, empty with 2 dichroic filter holders 850.00
1441006 S3e Filter Block B, empty with 2 dichroic filter holders 850.00
1441007 S3e Individual Filter Holder, black, empty 100.00
1441008 Neutral Density Filter, optical density 1.0 80.00
1441009 S3e Nozzle Tip, 100 µm 600.00
1441010 S3e Nozzle O-Ring with Alignment Disk, pkg of 2 58.00
1441011 S3e Collection Adaptor Set 290.00
1441012 Hex Driver, 2 mm 9.00
1441013 S3e Individual Dichroic Filter Holder, empty 100.00
1451076 S3e Power Conditioner, international 4,000.00
1451077 S3e Power Conditioner, U.S. 3,800.00
1451065 S3e Cell Sorter Accessory Kit, includes 100 μm nozzle tip, 2 nozzle O-rings, 2 nozzle alignment 1,000.00
disks, 1 ml syringe, 2 neutral density filters (1.0), 2 mm hex driver, spanner wrench
1451086 ProLine Universal Calibration Beads, includes 3 x 5 ml bottles of ready-to-use beads for alignment 490.00
verification and drop delay determination on the S3e cell sorter
1451082 ProFlow Sort Grade 8x Sheath Fluid, includes 5 x 4 L ready-to-use, sterile, preservative-free 490.00
phosphate buffered saline (PBS) solutions in gamma-irradiated S3e fluidic containers
1451083 roFlow Sort Grade Water, includes 5 x 4 L sterile, endotoxin-free water in gamma-irradiated
P 390.00
S3e fluidic containers
1451084 S3e Fluidic Container, 3 x 4 L gamma-irradiated sterile containers for use on the S3 or 78.00
S3e cell sorter
1451085 ProLine Rainbow Beads, includes a 5 ml bottle containing a mixture of beads dyed with 8 different 460.00
fluorescence intensities for excitation at wavelengths between 365–650 nm
Ordering Information
Catalog # Description Price, U.S. $
1451005 S3e Cell Sorter (488 nm), 488 nm 100 mW laser, includes 2 fluorescence detectors with filters, Inquire
2 fluidic containers with connectors, software
1451006 S3e Cell Sorter (488/561 nm), 488 nm and 561 nm 100 mW lasers, includes 4 fluorescence Inquire
detectors with filters, 2 fluidic containers with connectors, software
1451008 S3e Cell Sorter (488/640 nm), 488 nm and 640 nm 100 mW lasers, includes 4 fluorescence Inquire
detectors with filters, AutoGimbal system, ProSort software
1451078 S3e Biosafety System Class I Inquire
1451029 S3e Cell Sorter (488 nm) with S3e Biosafety System Class I Inquire
1451030 S3e Cell Sorter (488/561 nm) with S3e Biosafety System Class I Inquire
1451032 S3e Cell Sorter (488/640 nm) with S3e Biosafety System Class I Inquire
1451076 S3e Power Conditioner, international 4,000.00
1451077 S3e Power Conditioner, U.S. 3,800.00
Consumables
Ordering Information
Catalog # Description Price, U.S. $
1451085 ProLine Rainbow Beads, includes a 5 ml bottle containing a mixture of beads dyed with 8 460.00
different fluorescence intensities for excitation at wavelengths between 365–650 nm
1451086 ProLine Universal Calibration Beads, 15 ml (3 x 5 ml) 490.00
is available per case when it is used on the S3™ or S3e™ S3e fluidic container:
page 40.
cell sorter. The S3 or S3e cell sorter employs its internal
fluidics system to automatically dilute ProFlow sort grade
8x sheath fluid and creates 32 L of 1x sheath solution from
each container, generating 160 L per case.
Product features include:
■■
o manual dilution required — the S3 and S3e cell
N
sorters’ unique internal fluidics chamber automatically
generates a 1x solution, ensuring accurate dilution and
minimizing potential contamination
■■
asy container swapping — prepackaged, sterile S3e
E
fluidic container bypasses the need to fill a new container
during a sort, minimizing the risk of contamination
■■
inimum storage requirements — one 20 L case
M
creates 160 L of 1x sheath solution, freeing your storage
space for other purposes
For More Information
Web: bio-rad.com/sheath
Request or download bulletins: 6614 and #10030825
Ordering Information
Catalog # Description Price, U.S. $
1451082 ProFlow Sort Grade 8x Sheath Fluid, 20 L (5 x 4 L) 490.00
Ordering Information
Catalog # Description Price, U.S. $
1451083 roFlow Sort Grade Water, includes 5 x 4 L sterile, endotoxin-free water in gamma-irradiated
P 390.00
S3e fluidic containers
Ordering Information
Catalog # Description Price, U.S. $
1451084 S3e Fluidic Container, 3 x 4 L gamma-irradiated sterile containers for use on the 78.00
S3 or S3e cell sorter
Ordering Information
Catalog # Description Price, U.S. $
1351111 VivaFix 353/442 Cell Viability Assay, pkg of 1, kit includes 4 x 50 test vials and 250 µl of DMSO, 260.00
for running 200 assays
1351112 VivaFix 410/450 Cell Viability Assay, pkg of 1, kit includes 4 x 50 test vials and 250 µl of DMSO, 260.00
for running 200 assays
1351113 VivaFix 408/512 Cell Viability Assay, pkg of 1, kit includes 4 x 50 test vials and 250 µl of DMSO, 260.00
for running 200 assays
1351114 VivaFix 398/550 Cell Viability Assay, pkg of 1, kit includes 4 x 50 test vials and 250 µl of DMSO, 260.00
for running 200 assays
1351115 VivaFix 498/521 Cell Viability Assay, pkg of 1, kit includes 4 x 50 test vials and 250 µl of DMSO, 260.00
for running 200 assays
1351116 VivaFix 547/573 Cell Viability Assay, pkg of 1, kit includes 4 x 50 test vials and 250 µl of DMSO, 260.00
for running 200 assays
1351117 VivaFix 583/603 Cell Viability Assay, pkg of 1, kit includes 4 x 50 test vials and 250 µl of DMSO, 260.00
for running 200 assays
1351118 VivaFix 649/660 Cell Viability Assay, pkg of 1, kit includes 4 x 50 test vials and 250 µl of DMSO, 260.00
for running 200 assays
120 120
Excitation/emission = 538/617 nm Excitation/emission = 548/648 nm
+ DNA in pH 7.0 buffer + DNA in pH 7.0 buffer
Intensity, absorbance unit (AU)
80 80
60 60
40 40
20 20
0 0
400 500 600 700 400 500 600 700 800
Wavelength, nm Wavelength, nm
fluorescence 20- to 30-fold after binding to double- for GC-rich regions of double-stranded DNA
stranded DNA/RNA ■■
Ready-to-use formulation in PBS without preservatives
■■
Ready-to-use formulation in PBS without preservatives ■■
Stable at room temperature
■■
Stable at room temperature ■■
Maximum excitation: 546 nm
■■
Maximum excitation: 535 nm ■■
Maximum emission: 647 nm
■■
Maximum emission: 617 nm For More Information
Web: bio-rad.com/readidrop1
Ordering Information
Catalog # Description Price, U.S. $
1351101 ReadiDrop Propidium Iodide, includes 3 x 3 ml bottles of ready-to-use ultra-pure grade propidium iodide 260.00
suspended in PBS for dead cell exclusion in cell sorting and flow cytometry applications
1351102 ReadiDrop 7-AAD, includes 3 x 3 ml bottles of ready-to-use 7-aminoactinomycin D (7-AAD) suspended in 260.00
PBS for dead cell exclusion in cell sorting and flow cytometry applications
culture media 80 80
Normalized to mode
Normalized to mode
■■
ix and permeabilize your cells for intracellular analysis
F 60 60
Ordering Information
Catalog # Description Price, U.S. $
1351202 CytoTrack Blue 403/454, pkg of 1, kit includes 4 vials blue dye and 1 vial DMSO for 200 cell proliferation tests 305.00
1351203 CytoTrack Green 511/525, pkg of 1, kit includes 4 vials green dye and 1 vial DMSO for 200 cell proliferation tests 305.00
1351204 CytoTrack Yellow 542/556, pkg of 1, kit includes 4 vials yellow dye and 1 vial DMSO for 200 cell proliferation tests 305.00
1351205 CytoTrack Red 628/660, pkg of 1, kit includes 4 vials red dye and 1 vial DMSO for 200 cell proliferation tests 305.00
100
CFDA-SE cell proliferation assay provides carboxyfluorescein
diacetate succinimidyl ester (CFDA-SE) in a convenient and 80
easy-to-use format. CFDA-SE is a cell-permeable dye that CFDA-SE excitation/
Intensity, AU
fluoresces after the acetate groups are cleaved by intracellular 60 emission is 492/517 nm
in 0.1 M NaOH. Excitation
esterases, creating carboxyfluorescein succinimidyl ester 40 wavelength (—); emission
(CFSE). As the succinimidyl ester group reacts with primary wavelength (—). AU,
amines of intracellular proteins, CFSE is retained in the cell 20
absorbance unit.
and is successively halved during cell divisions.
450 500 550 600 650
■■
Visualize up to 8 cell divisions Wavelength, nm
■■
inimize contamination and reduce dilution steps
M For More Information
with smaller vial size Web: bio-rad.com/cytotrack1
Ordering Information
Catalog # Description Price, U.S. $
1351201 FDA-SE Cell Proliferation Assay, includes 5 x 100 μg vials 5(6)-carboxyfluorescein diacetate
C 155.00
succinimidyl ester, CFDA-SE
Subject Index
Antibody Labeling Kits, 41
CFDA-SE Cell Proliferation Assay, 44
CytoTrack™ Cell Proliferation Assay, 44
ProFlow™ Sort Grade 8x Sheath Fluid, 39
ProFlow™ Sort Grade Water, 40
ProLine™ Rainbow Beads, 38
ProLine™ Universal Calibration Beads, 38
ReadiDrop™ Cell Viability Assays, 43
S3e™ Cell Sorter, 34
S3e™ Biosafety System Class I, 37
S3e™ Fluidic Container, 40
VivaFix™ Cell Viability Assay, 42
Cell Imaging
Bio-Rad’s suite of tools for fluorescence microscopy and cell imaging includes the ZOE fluorescent cell imager
and nuclear dyes.
Ordering Information
Catalog # Description Price, U.S. $
1450031 ZOE Fluorescent Cell Imager, 120–240 V, includes instrument, power supply, USB flash drive Inquire
Bio-Rad offers a variety of kits and reagents for use with the ZOE fluorescent cell imager.
Ordering Information
Catalog # Description Price, U.S. $
Nuclear Staining Dyes
1351303 PureBlu DAPI Nuclear Staining Dye, pkg of 1, includes 5 x 50 µg vials of DAPI powder 105.00
1351304 PureBlu Hoechst 33342 Nuclear Staining Dye, pkg of 1, includes 5 x 56 µg vials of Hoechst 33342 powder 105.00
Antibody Labeling Kits
1351001 ReadiLink 350/440 Antibody Labeling Kit, includes 2 vials of labeling dye, reaction buffer, 310.00
and dyed quench buffer for labeling 2 x 50 µg of antibodies
1351002 ReadiLink 492/516 Antibody Labeling Kit, includes 2 vials of labeling dye, reaction buffer, 310.00
and dyed quench buffer for labeling 2 x 50 µg of antibodies
1351003 ReadiLink 555/570 Antibody Labeling Kit, includes 2 vials of labeling dye, reaction buffer, 310.00
and dyed quench buffer for labeling 2 x 50 µg of antibodies
1351004 ReadiLink 594/610 Antibody Labeling Kit, includes 2 vials of labeling dye, reaction buffer, 310.00
and dyed quench buffer for labeling 2 x 50 µg of antibodiess
Fixable Viability Dyes
1351111 VivaFix 353/442 Cell Viability Assay, pkg of 1, kit includes 4 x 50 assay vials and 250 µl of DMSO, 260.00
for running 200 assays
1351115 VivaFix 498/521 Cell Viability Assay, pkg of 1, kit includes 4 x 50 assay vials and 250 µl of DMSO, 260.00
for running 200 assays
1351116 VivaFix 547/573 Cell Viability Assay, pkg of 1, kit includes 4 x 50 assay vials and 250 µl of DMSO, 260.00
for running 200 assays
1351117 VivaFix 583/603 Cell Viability Assay, pkg of 1, kit includes 4 x 50 assay vials and 250 µl of DMSO, 260.00
for running 200 assays
Ordering Information
Catalog # Description Price, U.S. $
1351304 PureBlu Hoechst 33342 Nuclear Staining Dye, pkg of 1, includes 5 x 56 µg vials of 105.00
Hoechst 33342 powder
Ordering Information
Catalog # Description Price, U.S. $
1351303 PureBlu DAPI Nuclear Staining Dye, pkg of 1, includes 5 x 50 µg vials of DAPI powder 105.00
Subject Index
Kits and Reagents for Cell Imaging, 49
PureBlu™ DAPI Nuclear Staining Dye, 50
PureBlu™ Hoechst 33342 Nuclear Staining Dye, 49
ZOE™ Fluorescent Cell Imager, 48
Chromatography Resins/Media 55
Ion Exchange Resins 57
Analytical Grade Ion Exchange Resins 61
Mixed-Mode Chromatography Resins/Media 65
Recombinant-Tagged Affinity Purification 80
Affinity Purification 78
Affinity Resins/Media 80
Activated Affinity Resins/Media 83
Size Exclusion Chromatography 85
Hydrophobic Interaction Chromatography 87
Resin and Cartridge Sampler Packs, 88
Kits, and Standards
Chromatography Columns 91
Prepacked Chromatography Columns 91
Empty Columns 103
Chromatography Overview
A Range of Options
Bio-Rad offers a wide selection of chromatography tools for the life scientist involved in analytical, preparative, or process
chromatography. We have become known as a quality provider of chromatography resins for reagent cleanup and
biomolecule purification, manufacturing flexible and intuitive instrumentation and software and prepacked and empty
columns for sample separations.
Throughput, capacity, selectivity, resolution, and process economics are among the considerations when selecting any
chromatography resin, column, or instrument. We offer products for each phase of purification and manufacture resins for
any scale from nanograms to kilograms.
Bio-Rad offers a wide range of lab and process chromatography resins for ion exchange, hydroxyapatite, affinity, size exclusion,
and hydrophobic interaction chromatography as well as chromatography standards. For convenient sample preparation
products, see page 2. Bio-Rad’s process chromatography resins are used worldwide to manufacture registered biotherapeutics
and diagnostics. All chromatography resins are manufactured in an ISO 9001 registered manufacturing facility. The manufacturing
processes are audited and registered by National Quality Assurance Limited against the provisions of ANSI/ISO/ASQ 9001:2000.
Development, Application, and Validation Support
Bio-Rad provides development and application support for large-scale separations. We also offer regulatory assistance and
regulatory support files to help validate the chromatography resins used in your processes. These files contain information essential
to validation, including general product information, specification test procedures, identification tests, and biological safety data.
Learn More about the Technology
Web: bio-rad.com/tech/chrom
Nuvia™ Resin, Nuvia™ cPrime™ Resin, Profinity™ Resin, Bio-Gel P Gel, Macro-Prep Methyl
pg. 57 pg. 65 pg. 72, 83 pg. 85 Resin, pg. 87
UNOsphere™ Resin, CHT™ Resin, Affi-Gel® Resin, Bio-Gel A Gel, Macro-Prep t-Butyl
pg. 57 pg. 66 pg. 79 pg. 86 Resin, pg. 87
Macro-Prep® Resin, MPC™ Resin, Affi-Prep® Resin, Bio-Beads S-X Resin, Bio-Beads SM-2,
pg. 59 pg. 67 pg. 79 pg. 86 pg. 87
UNO® Columns, CFT ™ Resin, Nuvia IMAC Resin, ENrich SEC Columns,
pg. 100 pg. 68 pg. 71 pg. 93
54 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Chromatography Resins/Media
bio-rad.com/chromresin
Chromatography Resins/Media
Bio-Rad offers process-scale developers a selection of resins (media) for protein separation using ion exchange, hydroxyapatite and
fluoroapatite, affinity, size exclusion (gel filtration), and hydrophobic interaction chromatography. These resins (media) offer high binding
capacity, base stability, high flow rates, and are available in multi-liter quantities.
For More Information
Request or download bulletin: 6713
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 55
Chromatography Resins/Media
bio-rad.com/chromresin
56 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Chromatography Resins/Media
bio-rad.com/ionexchange Ion Exchange Resins
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 57
Chromatography Resins/Media
Ion Exchange Resins bio-rad.com/ionexchange
Ordering Information
Catalog # Description U.S. $
1560311 Nuvia S Media, 25 ml Ultra-high capacity strong cation process media 105.06
1560313 Nuvia S Media, 100 ml 315.18
1560315 Nuvia S Media, 500 ml 1,163.01
156-0317 Nuvia S Media, 10 L Inquire
1560511 Nuvia HR-S Media, 25 ml High-resolution strong cation process media 99.81
1560513 Nuvia HR-S Media, 100 ml 327.79
1560515 Nuvia HR-S Media, 500 ml 1,365.78
1560517 Nuvia HR-S Media, 10 L Inquire
1560411 Nuvia Q Media, 25 ml Ultra-high capacity strong anion process media 106.09
1560413 Nuvia Q Media, 100 ml 315.18
1560415 Nuvia Q Media, 500 ml 1,163.01
156-0417 Nuvia Q Media, 10 L Inquire
1560101 UNOsphere Q Media, 25 ml High-capacity strong anion media 92.00
1560103 UNOsphere Q Media, 100 ml 285.00
1560105 UNOsphere Q Media, 500 ml 1,046.96
156-0107 UNOsphere Q Media, 10 L Inquire
1560111 UNOsphere S Media, 25 ml High-capacity strong cation media 92.00
1560113 UNOsphere S Media, 100 ml 285.00
1560115 UNOsphere S Media, 500 ml 1,046.96
156-0117 UNOsphere S Media, 10 L Inquire
58 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Chromatography Resins/Media
bio-rad.com/ionexchange Ion Exchange Resins
Properties of Macro-Prep
Property High Q DEAE High S CM 25 Q* 25 S
Type of resin Strong anion Weak anion Strong cation Weak cation Strong anion Strong cation
Functional ligand –N+ (CH3)3 –N+ (C2H5)2 –SO3– –COO– –N+(CH3)3 –SO3–
Ionic capacity 400 ± 75 µeq/ml 175 ± 75 µeq/ml 160 ± 40 µeq/ml 210 ± 40 µeq/ml 220 ± 40 µeq/ml 110 ± 30 µeq/ml
Typical binding capacity 40 mg BSA/ml 35 mg BSA/ml 70 mg IgG/ml 25 mg hemoglobin/ml >30 mg/ml BSA >40 mg/ml BSA
Counter ion Cl– Cl– Na+ Na+ Cl– Na+
Nominal particle size 50 µm 50 µm 50 µm 50 µm 25 µm 25 µm
Nominal pore size 1,000 Å 1,000 Å 1,000 Å 1,000 Å 725 Å 725 Å
Recommended linear — — — — 50–300 cm/hr 50–300 cm/hr
flow rate range
Maximum recommended 3,000 cm/hr 3,000 cm/hr 3,000 cm/hr 3,000 cm/hr 3,000 cm/hr 3,000 cm/hr
linear flow rate
Chemical stability
1% SDS, 24 hr • • • • • •
6 M guanidine-HCl, 24 hr • • • • • •
Volume changes
pH 4–10 <1% <1% <3% <1% <1% <1%
0.1–1.0 M NaCl <5% <5% <9% <4% <5% <5%
Autoclavability (121°C, 80 min) • • • • • •
pH stability 1–10** 1–10** 1–10** 1–12 1–10** 1–10**
Storage conditions 20% ethanol 20% ethanol 20% ethanol 20% ethanol 20% ethanol 20% ethanol
* Do not autoclave the OH– form.
** The use of basic reagents greater than pH 10 should be evaluated for each application.
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 59
Chromatography Resins/Media
Ion Exchange Resins bio-rad.com/ionexchange
Ordering Information
Catalog # Description Price, U.S. $
Macro-Prep High Q Resin/Media
1580040 Macro-Prep High Q Media, 25 ml High-capacity strong anion exchange media; 48.00
1560040 Macro-Prep High Q Media, 100 ml very high flow rates and resolution 155.00
1560041 Macro-Prep High Q Media, 500 ml 612.00
156-0042 Macro-Prep High Q Media, 5 L Inquire
156-0043 Macro-Prep High Q Media, 10 L Inquire
Macro-Prep DEAE Resin/Media
1580020 Macro-Prep DEAE Media, 25 ml High-capacity weak anion exchange media; very 48.00
1560020 Macro-Prep DEAE Media, 100 ml high flow rates and resolution 155.00
1560021 Macro-Prep DEAE Media, 500 ml 612.00
156-0022 Macro-Prep DEAE Media, 5 L Inquire
156-0023 Macro-Prep DEAE Media, 10 L Inquire
Macro-Prep High S Resin/Media
1580030 Macro-Prep High S Media, 25 ml High-capacity strong cation exchange media; 48.00
1560030 Macro-Prep High S Media, 100 ml very high flow rates and resolution 155.00
1560031 Macro-Prep High S Media, 500 ml Inquire
156-0032 Macro-Prep High S Media, 5 L Inquire
156-0033 Macro-Prep High S Media, 10 L Inquire
Macro-Prep CM Resin/Media
1580070 Macro-Prep CM Media, 25 ml High-capacity weak cation exchange media; 56.00
1560070 Macro-Prep CM Media, 100 ml high flow rates and resolution 170.00
1560071 Macro-Prep CM Media, 500 ml Inquire
156-0073 Macro-Prep CM Media, 10 L Inquire
Macro-Prep 25 Q Resin/Media
1530021 Macro-Prep 25 Q Media, 50 ml Strong anion exchange media; 25 µm bead that offers 655.00
1530022 Macro-Prep 25 Q Media, 200 ml higher resolution 2,355.00
153-0023 Macro-Prep 25 Q Media, 1 L Inquire
153-0024 Macro-Prep 25 Q Media, 5 L Inquire
Macro-Prep 25 S Resin/Media
1530031 Macro-Prep 25 S Media, 50 ml Strong cation exchange media; 25 µm bead that offers 655.00
1530032 Macro-Prep 25 S Media, 200 ml higher resolution 2,300.00
153-0033 Macro-Prep 25 S Media, 1 L Inquire
153-0034 Macro-Prep 25 S Media, 5 L Inquire
60 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Chromatography Resins/Media
bio-rad.com/agresins Analytical Grade Ion Exchange Resins
AG (analytical grade) resins — AG resins are primarily Bio-Rex resin — available as weak anion and
used for the separation of low MW compounds such cation exchangers and as monosized mixed-bed ion
as inorganic ions, organic acids, nucleic acids, or exchangers. Bio-Rex 70 resins are macroreticular
carbohydrates. They are available as both strong and resins with a high capacity for high molecular weight
weak cation and anion exchangers and as mixed-bed ion compounds used for the purification and fractionation
exchangers. Many are available in several ionic forms and of proteins and peptides
can be converted from one form to another
Chelex resins — contain paired iminodiacetate ions
■■
G 50W-X8 strong cation exchanger resins —
A coupled to a styrene divinylbenzene support. They are
particularly useful for the removal of ethidium bromide unique chelating resins that bind polyvalent cations with
and propidium iodide from DNA samples high selectivity and are used to remove metal ions from
■■
AG 501-X8 mixed-bed resins — useful for deionization samples and buffers
of water and nonelectrolyte solutions
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 61
Chromatography Resins/Media
Analytical Grade Ion Exchange Resins bio-rad.com/agresins
See Also
Ordering Information
Poly-Prep ion Nominal
exchange columns: Dry Mesh Wet Bead Shipping %
page 98.
Catalog # Description Ionic Form Size Size, µm Water Price, U.S. $
AG Resins
1401231 AG 1-X2 Resin, 500 g Chloride 50–100 180–500 70–78 625.00
1401241 AG 1-X2 Resin, 500 g Chloride 100–200 106–250 70–78 505.00
1401251 AG 1-X2 Resin, 500 g Chloride 200–400 75–180 70–78 505.00
1401253 AG 1-X2 Resin, 500 g Acetate 200–400 75–180 70–78 775.00
1401331 AG 1-X4 Resin, 500 g Chloride 50–100 180–425 59– 65 460.00
1401341 AG 1-X4 Resin, 500 g Chloride 100–200 106–250 59– 65 625.00
1401351 AG 1-X4 Resin, 500 g Chloride 200–400 63–150 59– 65 695.00
1401421 AG 1-X8 Resin, 500 g Chloride 20–50 300–1,180 39–45 355.00
1401431 AG 1-X8 Resin, 500 g Chloride 50–100 180–425 39–45 545.00
1401441* AG 1-X8 Resin, 500 g Chloride 100–200 106–180 39–45 545.00
1401451* AG 1-X8 Resin, 500 g Chloride 200–400 45–106 39–45 585.00
1401422 AG 1-X8 Resin, 500 g Hydroxide 20–50 300–1,180 39–45 440.00
1401443 AG 1-X8 Resin, 500 g Acetate 100–200 106–180 39–45 695.00
1401453 AG 1-X8 Resin, 500 g Acetate 200–400 45–106 39–45 595.00
1401444 AG 1-X8 Resin, 500 g Formate 100–200 106–180 39–45 595.00
1401454 AG 1-X8 Resin, 500 g Formate 200–400 45–106 39–45 595.00
1421231 AG 50W-X2 Resin, 500 g Hydrogen 50–100 300–1,180 75–83 1,050.00
1421241* AG 50W-X2 Resin, 500 g Hydrogen 100–200 106–300 75–83 730.00
1421251 AG 50W-X2 Resin, 500 g Hydrogen 200–400 75–180 75–83 1,290.00
1421331 AG 50W-X4 Resin, 500 g Hydrogen 50–100 180–425 64–72 580.00
1421341 AG 50W-X4 Resin, 500 g Hydrogen 100–200 106–250 64–72 590.00
1421351* AG 50W-X4 Resin, 500 g Hydrogen 200–400 63–150 64–72 560.00
1421421 AG 50W-X8 Resin, 500 g Hydrogen 20–50 300–1,180 50–56 460.00
1421431 AG 50W-X8 Resin, 500 g Hydrogen 50–100 180–425 50–56 730.00
1421441*,** AG 50W-X8 Resin, 500 g Hydrogen 100–200 106–250 50–56 620.00
1421451*,** AG 50W-X8 Resin, 500 g Hydrogen 200–400 63–150 50–56 735.00
1421641 AG 50W-X12 Resin, 500 g Hydrogen 100–200 106–250 42–48 1,295.00
1421651 AG 50W-X12 Resin, 500 g Hydrogen 200–400 53–106 42–48 2,060.00
1411831 AG MP-1M Resin, 500 g Chloride 50–100 150–300 56–64 745.00
1411841 AG MP-1M Resin, 500 g Chloride 100–200 75–150 56–64 795.00
1411851 AG MP-1M Resin, 500 g Chloride 200–400 38–75 56–64 1,075.00
1430841 AG MP-50 Resin, 500 g Hydrogen 100–200 75–150 46–52 865.00
1404341* AG 4-X4 Resin, 500 g Free base 100–200 75–150 — 545.00
1426424*,**,*** AG 501-X8 Resin, 500 g H+ + OH– 20–50 300–1,180 43–55 360.00
1426425*,** AG 501-X8(D) Resin, 500 g H+ + OH– 20–50 300–1,180 43–55 510.00
1427834* AG 11 A8 Resin, 500 g Self-adsorbed 50–100 180–425 — 560.00
Bio-Rex Resins
1407841 Bio-Rex 5 Resin, 500 g Chloride 100–200 75–150 50–58 975.00
1407851 Bio-Rex 5 Resin, 500 g Chloride 200–400 45–75 50–58 1,510.00
1425822 Bio-Rex 70 Resin, 500 g Sodium 20–50 300–1,180 65–74 795.00
1425832* Bio-Rex 70 Resin, 500 g Sodium 50–100 150–300 65–74 795.00
1425842 Bio-Rex 70 Resin, 500 g Sodium 100–200 75–150 65–74 795.00
1425852* Bio-Rex 70 Resin, 500 g Sodium 200–400 45–75 65–74 795.00
1427425* Bio-Rex MSZ 501(D) H+ + OH– 25–35 500–700 — 435.00
Resin, 500 g
Larger volumes and special packaging for industrial applications are available on request.
Chelex Resins
1422822 Chelex 100 Resin, 500 g Sodium 50–100 300–1,180 68–76 790.00
1422832* Chelex 100 Resin, 500 g Sodium 100–200 150–300 68–76 790.00
1422842** Chelex 100 Resin, 500 g Sodium 200–400 75–150 68–76 950.00
1422825 Chelex 100 Resin, 100 g Iron 100–200 150–300 — 790.00
* Also available as biotechnology grade resin.
** Also available as molecular biology grade resin.
*** Also available as reactor grade resin.
continues
62 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Chromatography Resins/Media
bio-rad.com/agresins Analytical Grade Ion Exchange Resins
Ordering Information
Catalog # Description Price, U.S. $
AG Resins in Larger Volumes and Special Packaging for Industrial Applications
1401255 AG 1-X2 Resin, acetate, 200–400 mesh, 10 kg Inquire
1401342 AG 1-X4 Resin, chloride, 100–200 mesh, 10 kg Inquire
140-1445 AG 1-X8 Resin, chloride, 100–200 mesh, 10 kg Inquire
140-1424 AG 1-X8 Resin, hydroxide, 20–50 mesh, 10 kg Inquire
140-2341 AG 4-X4 Resin, free base, 100–200 mesh, 5 kg Inquire
142-1424 AG 50W-X8 Resin, ultrapure, hydrogen, 20–50 mesh, 10 kg Inquire
142-1423 AG 50W-X8 Resin, hydrogen, 20–50 mesh, 10 kg Inquire
142-1442 AG 50W-X8 Resin, hydrogen, 100–200 mesh, 10 kg Inquire
142-1254 AG 50W-X12 Resin, hydrogen, 200–400 mesh, 1 kg Inquire
141-1842 AG MP-1M Resin, chloride, 100–200 mesh, 10 kg Inquire
141-1853 AG MP-1M Resin, nitrate, 200–400 mesh, 10 kg Inquire
143-6427* AG 501-X8(D) Resin, H+ + OH–, 20–50 mesh, 10 kg Inquire
Bio-Rad offers reactor grade Bio-Rex® RG 501-X8 resins for power plant deionization systems and large-scale cleanup
of metals from waste water.
For More Information
Web: bio-rad.com/agresins
Ordering Information
Catalog # Description Ionic Form Dry Mesh Size MW Exclusion Price, U.S. $
Technical and Reactor Grade Resins
444-9998* Bio-Rex RG 501-X8 Resin, 1 ft3, for water purification H+ + OH – 20–50 1,000 Inquire
4449999 Bio-Rex RG 501-X8 Resin, 500 g H+ + OH – 20–50 1,000 120.00
* 1 ft3 corresponds to approximately 20 kg. Larger volumes and special packaging are available on request.
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 63
Chromatography Resins/Media
Analytical Grade Ion Exchange Resins bio-rad.com/agresins
Ordering Information
Dry Mesh Wet Bead
Catalog # Description Ionic Form Size Size, µm Application Price, U.S. $
Molecular Biology Grade Resins
1436424 AG 501-X8 Resin, H+ + OH– 20–50 300–1,180 Deionization 455.00
molecular biology grade, 100 g
1436425 AG 501-X8(D) Resin, H+ + OH– 20–50 300–1,180 ~1,000 MW limit 470.00
molecular biology grade, 100 g
1421253 Chelex 100 Resin, Sodium 200–400 75–150 DNA extraction for PCR 195.00
molecular biology grade, 50 g sample preparation
Biotechnology Grade Resins
1431255 AG 1-X2 Resin, Hydroxide 200–400 75–180 Separation of small peptides, 755.00
biotechnology grade, 100 g nucleotides, and large metal
complexes
1431345 G 1-X4 Resin,
A Hydroxide 100–200 63–150 Separation of organic acids, 445.00
biotechnology grade, 100 g nucleotides, phosphoinositides,
and other anions
1432445 G 1-X8 Resin,
A Hydroxide 100–200 106–180 Separation of inorganic and 825.00
biotechnology grade, 100 g organic anions with MW <1,000
1432446 AG 1-X8 Resin, Hydroxide 200–400 45–106 460.00
biotechnology grade, 100 g
1435241 AG 50W-X2 Resin, Hydrogen 100–200 106–300 Separation of peptides, 400.00
biotechnology grade, 100 g nucleotides, and cations
1435341 AG 50W-X4 Resin, Hydrogen 200–400 75–150 Separation of amino acids, 400.00
biotechnology grade, 100 g nucleotides, and cations
1435441 AG 50W-X8 Resin, Hydrogen 100-200 106-250 Separation of amino acids 400.00
biotechnology grade, 100 g and cations
1435451 AG 50W-X8 Resin, Hydrogen 200–400 63–150 400.00
biotechnology grade, 100 g
1437834 AG 11 A8 Resin, Self- 50–100 180–425 Removal of ionic compounds 400.00
biotechnology grade, 100 g adsorbed
1437424 AG 501-X8 Resin, H+ + OH– 20–50 300–1,180 Deionization 400.00
biotechnology grade, 100 g
1437425 AG 501-X8(D) Resin, H+ + OH– 20–50 300–1,180 400.00
biotechnology grade, 100 g
1432832 Chelex 100 Resin, Sodium 100–200 150–300 PCR sample preparation 415.00
biotechnology grade, 100 g
1435832 Bio-Rex 70 Resin, Sodium 50–100 150–300 Separation of cationic proteins 400.00
biotechnology grade, 100 g and aminescz
1435852 Bio-Rex 70 Resin, Sodium 200–400 45–75 400.00
biotechnology grade, 100 g
1528920 Bio-Beads SM-2, 25 g — — — Detergent removal 125.00
1523920 Bio-Beads SM-2, 100 g — — — Detergent removal 465.00
64 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Chromatography Resins/Media
bio-rad.com/mixedmode Mixed-Mode Chromatography Resins/Media
Ordering Information
Catalog # Description Price, U.S. $
1563401 Nuvia cPrime Media, 25 ml 141.84
1563402 Nuvia cPrime Media, 100 ml 471.76
1563403 Nuvia cPrime Media, 500 ml Inquire
156-3404 Nuvia cPrime Media, 1 L Inquire
156-3405 Nuvia cPrime Media, 5 L Inquire
156-3406 Nuvia cPrime Media, 10 L Inquire
7324722 Foresight Nuvia cPrime Column, 1 ml 141.95
7324742 Foresight Nuvia cPrime Column, 5 ml 278.22
7324705 Foresight Nuvia cPrime Plates, 20 µl* 551.25
7324807 Foresight Nuvia cPrime RoboColumn Unit, 200 µl** 231.53
7324808 Foresight Nuvia cPrime RoboColumn Unit, 600 µl** 277.83
* Package size: 2 x 96-well plates.
** Package size: 1 row of 8 columns.
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 65
Chromatography Resins/Media
Mixed-Mode Chromatography Resins/Media bio-rad.com/mixedmode
Hydroxyapatite (Ca 5(PO4)3OH) 2 and fluoroapatite Ca10(PO4) 6F2 are forms of calcium phosphate that offer unique selectivities
and often separate biomolecules that appear homogeneous when other chromatographic and electrophoretic techniques
are used. Hydroxyapatite and fluoroapatite chromatography can be used at any stage from initial capture to final polishing.
Ordering Information
Description Type I Price, U.S. $ Type II Price, U.S. $
CHT Ceramic Hydroxyapatite, 20 µm
20 µm particle size, 10 g 1582000 135.00 1582200 135.00
20 µm particle size, 100 g 1570020 925.00 1572000 1,070.00
20 µm particle size, 1 kg (1.6 L) 157-0021 Inquire 157-2100 Inquire
CHT Ceramic Hydroxyapatite, 40 µm
40 µm particle size, 10 g 1584000 135.00 1584200 135.00
40 µm particle size, 100 g 1570040 870.00 1574000 930.00
40 µm particle size, 1 kg (1.6 L) 157-0041 Inquire 157-4100 Inquire
40 µm particle size, 5 kg (7.9 L) 157-0045 Inquire 157-4500 Inquire
CHT Ceramic Hydroxyapatite, 80 µm
80 µm particle size, 10 g 1588000 135.00 1588200 135.00
80 µm particle size, 100 g 1570080 880.00 1578000 835.00
80 µm particle size, 1 kg (1.6 L) 157-0081 Inquire 157-8100 Inquire
80 µm particle size, 5 kg (7.9 L) 157-0085 Inquire 157-8500 Inquire
CHT Foresight Plates, Columns, and RoboColumn Units
Foresight CHT Plate, 40 µm, 20 µl* 7324716 551.25 7324718 551.25
Foresight CHT Column, 40 µm, 1 ml 7324735 148.71 7324736 148.71
Foresight CHT Column, 40 µm, 5 ml 7324755 291.46 7324756 291.46
Foresight CHT RoboColumn Unit, 40 µm, 200 µl** 7324822 231.53 7324825 231.53
Foresight CHT RoboColumn Unit, 40 µm, 600 µl** 7324823 277.83 7324826 277.83
* Package size: 2 x 96-well plates.
** Package size: 1 row of 8 columns.
66 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Chromatography Resins/Media
bio-rad.com/mixedmode Mixed-Mode Chromatography Resins/Media
Ordering Information
Description 1 x 5 ml Price, U.S. $ 5 x 5 ml Price, U.S. $
CHT Prepacked Bio-Scale Mini Cartridges
CHT Type I Ceramic Hydroxyapatite 7324322 155.00 7324324 615.00
CHT Type II Ceramic Hydroxyapatite 7324332 155.00 7324334 615.00
Larger volumes and special packaging for industrial applications are available on request.
Ordering Information
Description Type I Price, U.S. $
MPC Ceramic Hydroxyfluoroapatite, 40 µm
40 µm particle size, 10 g 1580200 130.00
40 µm particle size, 100 g 1570200 850.00
40 µm particle size, 1 kg (1.4 L) 157-0201 Inquire
40 µm particle size, 5 kg (6.9 L) 157-0205 Inquire
MPC Foresight Plates, Columns, and RoboColumn Units
Foresight MPC Type I Plate, 20 μg* 7324785 551.25
Foresight MPC Type I Column, 40 µm, 1 ml 7324737 149.00
Foresight MPC Type I Column, 40 µm, 5 ml 7324757 291.06
Foresight MPC Type I RoboColumn Unit, 40 µm, 200 µl** 7324828 231.53
Foresight MPC Type I RoboColumn Unit, 40 µm, 600 µl** 7324829 264.60
* Package size: 2 x 96-well plates.
** Package size: 1 row of 8 columns.
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 67
Chromatography Resins/Media
Mixed-Mode Chromatography Resins/Media bio-rad.com/mixedmode
CFT ceramic fluoroapatite (Ca10(PO4)6F2) is a rigid spherical CFT has high binding capacity and may be used
macroporous media used in the purification of biologically reproducibly over an extended number of chromatography
significant compounds. CFT is a composite of fluoroapatite runs. Its increased tensile strength, chemical durability,
and hydroxyapatite prepared by chemically converting and density provide excellent throughput and
hydroxyapatite nanocrystals to fluoroapatite with a consistent performance for all separations, including
fluorine reagent. biopharmaceutical process-scale manufacturing.
CFT possesses separation characteristics similar to those of Features of CFT include:
CHT ceramic hydroxyapatite. However, when CFT is used, ■■ Acidic
protein separation for applications requiring
purification can be performed across a range of lower pH values pH as low as 5.6
to obtain optimal results for the targeted biomolecule. CFT Type ■■
High-density particles for fast, simple column packing
II is available in a 40 µm size and is sintered at high temperatures
to produce a physically and chemically stable media. ■■ Sintering at high temperatures for heavy-duty, durable media
■■
Rigid particles for fast cleaning and equilibration
CFT can be used under stringent chromatography
conditions to separate acidic proteins requiring buffered ■■
Inorganic calcium phosphate for distinct selectivities
conditions as low as pH 5.6 with minimal compromise to For More Information
the solubility or lifespan of the media. Web: bio-rad.com/CFT
Request or download bulletins: 3111 and 5853
Properties of CFT
Property Description
Functional groups Ca 2+, PO43–, F –
Particle sizes 40 ± 4 µm
Recommended linear flow rate 300 cm/hr
Operating pH range 5–14
Chemical compatibility 2 M NaOH, 6 M guanidine-HCl, 8 M urea, 0.1 M sodium acetate, pH 5.7
Regeneration
Normal conditions 400 mM sodium phosphate, pH 7.4
Difficult conditions 400–1,000 mM sodium phosphate, pH 11–12
Sanitization 1–2 M NaOH or KOH
Autoclavability (121ºC, 20 min) Yes
Packing density (g/ml packed bed) 0.86 g/ml
Dynamic binding capacity lysozyme (Lys) 14–21.5 mg Lys/g
Typical IgG binding capacities at 300 cm/hr 33 mg/ml
Nominal pore diameter 600–800 Å
Maximum operating pressure 55 bar (800 psi)
Note: A small amount (up to 5 mM) of sodium phosphate should be added to all unbuffered solutions as a counterion.
Ordering Information
Catalog # Description Price, U.S. $
CFT Ceramic Fluoroapatite, 20 µm
1585200 40 µm particle size, 10 g 585.00
1575000 40 µm particle size, 100 g 1,660.00
157-5100 40 µm particle size, 1 kg (1.2 L) Inquire
157-5500 40 µm particle size, 5 kg (5.8 L) Inquire
68 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Chromatography Resins/Media
bio-rad.com/mixedmode Mixed-Mode Chromatography Resins/Media
Ordering Information
Catalog # Description Price, U.S. $
Bio-Gel Hydroxyapatite
1300150 Bio-Gel HT Hydroxyapatite, hydrated, 250 ml 315.00
1300151 Bio-Gel HT Hydroxyapatite, hydrated, 500 ml 545.00
1300420 Bio-Gel HTP Hydroxyapatite, powder, 100 g 345.00
130-0421 Bio-Gel HTP Hydroxyapatite, powder, 1 kg Inquire
130-0425 Bio-Gel HTP Hydroxyapatite, powder, 5 kg Inquire
1300520 Bio-Gel HTP Hydroxyapatite, DNA grade, 100 g 620.00
Accessories
7376201 cono-Column Open-Ended Jacketed Chromatography Column, 1 x 30 cm,
E 420.00
includes 2 flow adaptors for DNA hydroxyapatite chromatography, 25 ml
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 69
Chromatography Resins/Media
Recombinant-Tagged Affinity Purification bio-rad.com/affinityresin
Ordering Information
Catalog # Description Price, U.S. $
7800811 Bio-Scale Mini Nuvia IMAC cartridges, 5 ml 160.00
7800812 Bio-Scale Mini Nuvia IMAC cartridges, 5 x 5 ml 600.00
70 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Chromatography Resins/Media
bio-rad.com/affinityresin Recombinant-Tagged Affinity Purification
Nuvia IMAC resin is an ultra high capacity, next-generation Nuvia IMAC resin delivers value and flexibility
affinity chromatography resin. Nuvia IMAC resin delivers by providing users:
high binding capacity over a range of pH and flow rates, ■■
Superior quality, reproducible separations
providing wide experimental design space for many ■■
High capacity, even at high flow rates
applications. Immobilized metal affinity chromatography ■■
Stability at pH 2–14
(IMAC) is a powerful purification technique that relies on the ■■
Biomolecule purification in a variety of workflows
affinity that the histidine tag has for immobilized transition ■■
Excellent single-step purity by affinity chromatography
metals. Nuvia IMAC resins, based on Nuvia beads, contain ■■
Quick, efficient separation of biomolecules
nitrilotriacetic acid (NTA) as the chelating ligand for di- or ■■
Contaminant removal
trivalent metal ions. Its chemical structure allows highly ■■
Robust base matrix
selective binding of recombinant histidine-tagged proteins
when charged with Ni2+ or other transition metals, such as For More Information
Web: bio-rad.com/nuviaIMACresin
Zn2+ or Cu2+. Due to superior mass transfer characteristics,
Nuvia IMAC resin offers high capacity at high flow rates.
It can be used under either nondenaturing or denaturing
conditions. Nuvia IMAC resin is available in 25, 100, and
500 ml bottles.
Ordering Information
Catalog # Description Properties (all listed products) Price, U.S. $
7800800 Nuvia IMAC Resin, 25 ml • Ultra-high capacity at high flow rates 300.00
7800801 Nuvia IMAC Resin, 100 ml • pH stability across broad range 1,010.00
7800802 Nuvia IMAC Resin, 500 ml • Robust beads Inquire
Larger volumes and special packaging are available on request. Please contact local sales specialist.
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 71
Chromatography Resins/Media
Recombinant-Tagged Affinity Purification bio-rad.com/affinityresin
Ordering Information
Catalog # Description Properties (all listed products) Price, U.S. $
Ready-to-Use Affinity Resins/Media
1560121 Profinity IMAC Uncharged Resin, 10 ml • Stable from pH 1 to 14 81.00
1560123 Profinity IMAC Uncharged Resin, 50 ml • Strong bead matrix for gravity flow 350.00
1560125 Profinity IMAC Uncharged Resin, 500 ml 3,065.00
1560127 Profinity IMAC Uncharged Resin, 1 L Inquire
1560131 Profinity IMAC Ni-Charged Resin, 10 ml 100.00
1560133 Profinity IMAC Ni-Charged Resin, 25 ml 245.00
1560135 Profinity IMAC Ni-Charged Resin, 100 ml 905.00
1560137 Profinity IMAC Ni-Charged Resin, 500 ml Inquire
72 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Chromatography Resins/Media
bio-rad.com/affinityresin Recombinant-Tagged Affinity Purification
Ordering Information
Catalog # Description Price, U.S. $
7324620 Bio-Scale Mini Profinity GST Cartridges, 5 x 1 ml 405.00
7324622 Bio-Scale Mini Profinity GST Cartridge, 1 x 5 ml 390.00
7324624 Bio-Scale Mini Profinity GST Cartridges, 5 x 5 ml 1,370.00
6200240 GST Buffer Kit, includes GST lysis, wash, and elution buffers 155.00
6200223 Profinia GST Buffer Kit, includes lysis, wash, and elution buffers, cleaning and storage solutions, 395.00
glutathione reagent; sufficient for 10 applications
6200243 GST Purification Kit, 1 ml, includes GST purification buffer kit and 2 x 1 ml GST cartridges 330.00
6200244 GST Purification Kit, 5 ml, includes 2 GST purification buffer kits and 1 x 5 ml GST cartridge 515.00
6200226 Profinia GST Purification Kit, 1 ml, includes Profinia GST buffer kit, 2 x 1 ml GST and 575.00
2 x 10 ml desalting cartridges
6200236 Profinia GST Purification Kit, 5 ml, includes 2 Profinia GST buffer kits, 1 x 5 ml GST and 1,095.00
1 x 50 ml desalting cartridge
6200230 Profinia GST Starter Kit, includes Profinia GST buffer kit, 1 x 1 ml GST and 1 x 10 ml desalting cartridge, 510.00
E. coli lysate, glutathione reagent
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 73
Chromatography Resins/Media
Recombinant-Tagged Affinity Purification bio-rad.com/affinity
See Also Profinity eXact Purification Starter Kit Profinity eXact pPAL7 Supercoiled Expression
Bio-Scale Mini
The Profinity eXact expression and purification starter kit Vector Kit
cartridges: can be used to easily evaluate Profinity eXact fusion-tag The pPAL7 supercoiled plasmid expression vector is
page 95.
technology. It is suitable for new purifications requiring tag used for routine cloning of target DNA sequences using
removal and for purifications where cleavage has resulted a conventional restriction digest cloning strategy.
in inferior results.
Profinity eXact Antibody Reagent
■■ Profinity eXact mini spin purification starter kit — The Profinity eXact antibody reagent is a mouse
for single-step purification and on-column cleavage of monoclonal antibody used to detect expression of the
the tagged protein; also includes a lyophilized lysate to target protein. The Profinity eXact antibody specifically
ensure that preliminary purifications are a success recognizes the prodomain of the subtilisin protease,
which is fused to the protein of interest. For convenient
western blot detection of the fusion protein, Bio-Rad
offers colorimetric detection kits.
Resin Specifications
Functional ligand Subtilisin protease, 27.8 kD
Base bead 6% agarose bead
Form 50% suspension in 100 mM sodium phosphate (pH 7.2), 0.02% sodium azide
Particle size range 60–160 µm
Dynamic binding capacity* >3 mg tag-free protein/ml resin
Recommended linear flow rate 1,000 cm/hr at 25°C
pH stability 2–13
Chemical compatibility Common reagents, including detergents, reducing agents, buffering agents, and additives
Storage 4°C
Shelf life in 20% ethanol >1 year at 4°C
Operational temperature 4–40°C
* Dynamic binding capacity determination of a 40 kD maltose binding protein.
Note: Dynamic binding capacity is protein dependent.
Ordering Information
Catalog # Description Price, U.S. $
1563007 Profinity eXact Mini Spin Columns, includes 10 spin columns, ten 2 ml capped tubes, 165.00
and ten 2 ml capless tubes
7324646 Bio-Scale Mini Profinity eXact Cartridges, 2 x 1 ml 215.00
7324647 Bio-Scale Mini Profinity eXact Cartridges, 4 x 1 ml 370.00
7324648 Bio-Scale Mini Profinity eXact Cartridge, 1 x 5 ml 400.00
1563002 Profinity eXact pPAL Supercoiled Expression Vector Kit, includes 100 µl of 100 ng/µl vector, 20 reactions 215.00
1563004 Profinity eXact Monoclonal Antibody, 100 µl, 1 mg/ml 305.00
1563005 Profinity eXact Purification Resin,* 10 ml 555.00
1563006 Profinity eXact Mini Spin Purification Starter Kit, includes 10 prepacked spin columns, 50 x 2 ml collection 485.00
tubes, lyophilized control protein lysate, bacterial lysis reagent, 50 ml bind/wash buffer, 20 ml elution buffer
* Larger volumes are available on request.
74 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Chromatography Resins/Media
bio-rad.com/affinity Recombinant-Tagged Affinity Purification
Buffers Reagents
Bio-Rad premade affinity buffer kits for recombinant- Individually packaged reagents are available for use with
tagged protein purification come with concentrated formula the Profinia system. While designed specifically to work
sufficient for ten purifications for a 1 ml affinity cartridge. with the Profinia system, these reagents also have general
These kits provide easy and fast purification for histidine- applicability within all stages of an affinity purification
tagged (native IMAC buffer) proteins. workflow, from sample preparation to protein detection.
■■
enaturing IMAC purification can be performed with
D Histidine and GST Purification E. coli Control Lysate
native buffer IMAC kits with the addition of urea in the The histidine and GST purification E. coli control lysate is a
purification buffers lyophilized, dual-tagged 51 kD target protein that is meant
■■
its for desalting and cartridge cleaning after affinity
K to eliminate concerns over variability in purification buffer
purification are also available solutions or in the purification matrix itself. The control
lysate facilitates system setup and initial purification;
■■
urification kits containing both buffer kits and
P
it is included in every Profinia IMAC and GST starter kit.
appropriate cartridges are provided, if purification
cartridges are desired Glutathione Reagent
For More Information
Powdered glutathione reagents are available for use with GST
Request or download bulletins: 3193, 5283, 5444, and 5456 applications. The glutathione pack is essential for eluting
GST fusion proteins from immobilized glutathione resins.
Kits
Buffer and starter kits can be used with any instrument, Histidine and GST Antibodies
gravity-flow, or spin-column purification procedure. Histidine-tagged and GST-tagged monoclonal antibodies
are used to detect target protein expression of overexpressed
Profinia purification, buffer, and starter kits are designed 6x histidine and GST fusion proteins. They are supplied at
specifically for use on the Profinia protein purification a concentration of 1 mg/ml in phosphate buffered saline
system for time savings and highly reproducible results. (pH 7.4) with 0.05% NaN3.
These kits are directly installed on the system; manual
dilution of the concentrated buffers provided in the kits is
not required.
For More Information
Request or download bulletin: 5574
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 75
Chromatography Resins/Media
Recombinant-Tagged Affinity Purification bio-rad.com/affinity
See Also
Ordering Information
Profinity IMAC Catalog # Description Price, U.S. $
resins: page 72.
Affinity Buffer Kits
6200239 Native IMAC Buffer Kit, includes native IMAC lysis, wash, and elution buffers 135.00
6200240 GST Buffer Kit, includes GST lysis, wash, and elution buffers 155.00
6200224 Desalting and Cartridge Cleaning Buffer Kit, includes desalting buffer, cleaning buffers, and cartridge storage buffer 240.00
Affinity Purification Kits
6200241 Native IMAC Purification Kit, 1 ml, includes IMAC purification buffer kit and 2 x 1 ml IMAC cartridges 255.00
6200242 Native IMAC Purification Kit, 5 ml, includes 2 IMAC purification buffer kits and 1 x 5 ml IMAC cartridge 370.00
6200243 GST Purification Kit, 1 ml, includes GST purification buffer kit and 2 x 1 ml GST cartridges 330.00
6200244 GST Purification Kit, 5 ml, includes 2 GST purification buffer kits and 1 x 5 ml GST cartridge 515.00
Profinia Buffer Kits
6200221 Profinia Native IMAC Buffer Kit, includes lysis, wash, and elution buffers, cleaning and storage solutions; 350.00
sufficient for 10 applications
6200223 Profinia GST Buffer Kit, includes lysis, wash, and elution buffers, cleaning and storage solutions, glutathione 395.00
reagent; sufficient for 10 applications
Profinia Purification Kits
6200225 Profinia IMAC Purification Kit, 1 ml, includes Profinia native IMAC buffer kit, 2 x 1 ml IMAC and 515.00
2 x 10 ml desalting cartridges
6200235 Profinia IMAC Purification Kit, 5 ml, includes 2 Profinia native IMAC buffer kits, 1 x 5 ml IMAC 880.00
and 1 x 50 ml desalting cartridge
6200226 Profinia GST Purification Kit, 1 ml, includes Profinia GST buffer kit, 2 x 1 ml GST and 2 x 10 ml 575.00
desalting cartridges
6200236 Profinia GST Purification Kit, 5 ml, includes 2 Profinia GST buffer kits, 1 x 5 ml GST and 1 x 50 ml 1,095.00
desalting cartridge
6200228 Profinia Desalting Purification Kit, 10 ml, includes desalting and cartridge cleaning buffer kit, 265.00
2 x 10 ml desalting cartridges
6200238 Profinia Desalting Purification Kit, 50 ml, includes 2 desalting and cartridge cleaning buffer kits, 660.00
1 x 50 ml desalting cartridge
continues
76 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Chromatography Resins/Media
bio-rad.com/affinity Recombinant-Tagged Affinity Purification
Ordering Information
Catalog # Description Price, U.S. $
Profinia Purification Kits
6200229 Profinia Native IMAC Starter Kit, includes Profinia native IMAC buffer kit, 1 x 1 ml IMAC and 1 x 10 ml 530.00
desalting cartridge, E. coli lysate
6200230 Profinia GST Starter Kit, includes Profinia GST buffer kit, 1 x 1 ml GST and 1 x 10 ml desalting cartridge, 510.00
E. coli lysate, glutathione reagent
Bio-Scale Mini Affinity and Desalting Cartridges
7324610 Bio-Scale Mini Profinity IMAC Cartridges, 5 x 1 ml 225.00
7324612 Bio-Scale Mini Profinity IMAC Cartridge, 1 x 5 ml 155.00
7324614 Bio-Scale Mini Profinity IMAC Cartridges, 5 x 5 ml 540.00
7324620 Bio-Scale Mini Profinity GST Cartridges, 5 x 1 ml 405.00
7324622 Bio-Scale Mini Profinity GST Cartridge, 1 x 5 ml 390.00
Affinity Purification Kit Components
6200205 2x Native IMAC Lysis Buffer, 125 ml 78.00
6200206
2x Native IMAC Wash Buffer 1, 125 ml 52.00
6200207 2x Native IMAC Wash Buffer 2, 100 ml 45.00
6200208 2x Native IMAC Elution Buffer, 100 ml 50.00
6200213 2x GST Lysis Buffer, 100 ml 105.00
6200215 2x GST Elution Buffer, 100 ml 58.00
6200216 5x Desalting Buffer, 200 ml 58.00
6200217 2x Cleaning Solution 1, 125 ml 52.00
6200218 4x Cleaning Solution 2, 125 ml 87.00
6200219 2x Storage Solution, 200 ml 52.00
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 77
Chromatography Resins/Media
Affinity Purification bio-rad.com/affinitypurification
Affinity Purification
Ordering Information
Catalog # Description Price, U.S. $
UNOsphere SUPrA rProtein A Resin/Media
1560218 UNOsphere SUPrA rProtein A Media, 25 ml Inquire
1560219 UNOsphere SUPrA rProtein A Media, 100 ml Inquire
1560220 UNOsphere SUPrA rProtein A Media, 500 ml Inquire
78 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Chromatography Resins/Media
bio-rad.com/affinitypurification Affinity Purification
Chromatography on Affi-Gel and Affi-Prep Protein A resins In addition, the MAPS process and Affi-Gel Protein A
yield highly purified immunoglobulins (IgG), selectively permit greater binding of mouse IgG1 than does
remove IgG prior to analysis of other IgG classes, or adsorb immobilized Protein G resin.
immune complexes for antigen purification. Protein A binds For More Information
to the Fc region of immunoglobulins, especially IgG from Web: bio-rad.com/affigelproteina
mammalian species. Advantages include:
■■
High purity of IgGs Capacities of Protein A Resins
■■
High affinity for mammalian IgG Affi-Gel Protein A Affi-Prep Protein A
Immunoglobulin Capacity, mg/ml Capacity, mg/ml
■■ Highcapacities for mouse IgG1 as well as other
Mouse IgG1 6–10 7–9
subclasses with MAPS optimized buffer Mouse IgG2a 6–10 7–9
Mouse IgG2b 6–10 7–9
In addition, Affi-Prep resins offer high linear flow rates up Mouse IgG3 6–10 7–9
to 2,000 cm/hr, pressure stability up to 1,000 psi (70 bar), Mouse IgM* 6–10 7–9
and high chemical stability, which allows sanitization with Human IgG 15 10–12
0.1 M NaOH. Sheep, cow, horse, goat,
rabbit, dog, pig IgG 8–10 7–9
With Affi-Gel and Affi-Prep Protein A resins and MAPS II * Approximately 50% of all mouse IgMs bind using the MAPS buffer system.
buffer, you can purify up to 10 mg of IgG1/ml of media.
This is 8–10 times higher than with standard methods.
Ordering Information
Catalog # Description Price, U.S. $
Affi-Prep and Affi-Gel Media
1560006 Affi-Prep Protein A Media, 5 ml 415.00
1560005 Affi-Prep Protein A Media, 25 ml 1,965.00
1536153 Affi-Gel Protein A Media, 5 ml 330.00
1536154 Affi-Gel Protein A Media, 50 ml Inquire
1536159 Affi-Gel Protein A MAPS II Kit, includes 5 ml Affi-Gel Protein A media, Affi-Gel Protein A MAPS II buffers, 470.00
1 x 10 cm Econo-Column column; enough to purify 500 mg of mouse IgG1
1536161 Protein A MAPS II Binding Buffer, makes 5 L 835.00
Prepacked Econo-Pac Columns
7322022 Econo-Pac Protein A Columns, prefilled with Affi-Gel Protein A media, 5 540.00
7322020 Econo-Pac Protein A Kit, 1 x 2 ml Affi-Gel Protein A column, 1 x 10 ml 10DG column, buffers 330.00
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 79
Chromatography Resins/Media
Affinity Resins/Media bio-rad.com/affinityresin
Affinity Resins/Media
Affi-Gel Blue resin is a cross-linked agarose bead with For More Information
covalently attached Cibacron Blue F3GA dye. The blue dye Web: bio-rad.com/affigelblue
functions as an ionic, hydrophobic, aromatic, or sterically
active binding site in various applications. Affi-Gel Blue
resin is ideally suited for albumin removal (using 50–100
mesh) and enzyme purification (using 100–200 mesh).
Proteins and peptides are bound and released with a high
degree of specificity by manipulating the composition of
the eluent buffers.
The resin is available in bottle and cartridge form. Bio-Scale Mini Affi-Gel Blue Cartridge Affi-Gel Blue Gel
Ordering Information
Catalog # Description Applications (all listed products) Price, U.S. $
1537301 Affi-Gel Blue Gel, 100 ml, 50–100 mesh • Albumin removal 545.00
1537302 Affi-Gel Blue Gel, 100 ml, 100–200 mesh • Enzyme purification 545.00
7324642 Bio-Scale Mini Affi-Gel Blue Cartridge, 1 x 5 ml, 140.00
cartridges packed with 100–200 mesh media
7324644 Bio-Scale Mini Affi-Gel Blue Cartridges, 5 x 5 ml, 480.00
cartridges packed with 100–200 mesh media
DEAE Affi-Gel Blue resin is a bifunctional affinity gel For More Information
containing Cibacron Blue F3GA dye covalently attached Web: bio-rad.com/affigelDEAE
to DEAE Bio-Gel® A resin. The dye binds albumin,
proteases, and other complement proteins; the DEAE
group binds remaining acidic proteins. Features of
DEAE Affi-Gel Blue resin include:
■■ Single-stepIgG purification from serum; the eluted IgG
contains a small amount of transferrin (samples are
diluted approximately fivefold)
■■ No detectable proteolytic activity in the eluted IgG fraction
■■ Economical alternative to Protein A affinity chromatography Bio-Scale Mini DEAE Affi-Gel Blue Cartridge DEAE Affi-Gel Blue Gel
■■
Available in bottle and cartridge form
Ordering Information
Catalog # Description Applications (all listed products) Price, U.S. $
1537307 DEAE Affi-Gel Blue Gel, 100 ml •A lbumin, proteases, and complement protein 650.00
removal
7324632 Bio-Scale Mini DEAE Affi-Gel Blue Cartridge, 1 x 5 ml • IgG purification 165.00
7324634 Bio-Scale Mini DEAE Affi-Gel Blue Cartridge, 5 x 5 ml 560.00
continues
80 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Chromatography Resins/Media
bio-rad.com/affinityresin Affinity Resins/Media
Ordering Information
Description 1 x 5 ml Price, U.S. $ 5 x 5 ml Price, U.S. $
Prepacked Bio-Scale Mini Cartridges
DEAE Affi-Gel Blue Gel 7324632 165.00 7324634 560.00
Affi-Gel Blue Gel 7324642 140.00 7324644 480.00
Ordering Information
Catalog # Description Price, U.S. $
1537304 CM Affi-Gel Blue Gel, 100 ml 650.00
Ordering Information
Catalog # Description Price, U.S. $
1536103 Affi-Gel Boronate Gel, 5 g 650.00
153-6104 Affi-Gel Boronate Gel, 50 g Inquire
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 81
Chromatography Resins/Media
Affinity Resins/Media bio-rad.com/affinityresin
Ordering Information
Catalog # Description Price, U.S. $
1560010 Affi-Prep Polymyxin Media, 25 ml 835.00
Ordering Information
Catalog # Description Price, U.S. $
1536173 Affi-Gel Heparin Media, 40 ml 525.00
82 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Chromatography Resins/Media
bio-rad.com/affinityresin Activated Affinity Resins/Media
Ordering Information
Catalog # Description Price, U.S. $
1560200 Profinity Epoxide Media, 5 g 120.00
1560201 Profinity Epoxide Media, 25 g 575.00
Ordering Information
Catalog # Description Price, U.S. $
1536099 Affi-Gel 10 Media, 25 ml 175.00
1536046 Affi-Gel 10 Media, 4 x 25 ml 545.00
153-1000 Affi-Gel 10 Media, 1 L Inquire
1536051 Affi-Gel 15 Media, 25 ml 195.00
1536052 Affi-Gel 15 Media, 4 x 25 ml 615.00
1536098 Affi-Gel 10/15 Combination, includes 2 x 25 ml Affi-Gel 10 media and 2 x 25 ml Affi-Gel 15 media 560.00
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 83
Chromatography Resins/Media
Activated Affinity Resins/Media bio-rad.com/affinityresin
Ordering Information
Catalog # Description Price, U.S. $
1536047 Affi-Gel Hz Hydrazide Media, 25 ml 315.00
1536060 Affi-Gel Hz Immunoaffinity Kit, includes 5 ml Affi-Gel Hz media, 2 x 25 mg Affi-Gel Hz oxidizer, 330.00
25 ml Affi-Gel Hz coupling buffer concentrate, 2 Econo-Pac 10DG desalting columns, 1 x 10 cm
Econo-Column column
1536054 Affi-Gel Hz 10x Coupling Buffer Concentrate, 500 ml 150.00
Ordering Information
Catalog # Description Price, U.S. $
1532401 Affi-Gel 102 Media, 50 ml 305.00
1530990 EDAC, 5 g 200.00
84 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Chromatography Resins/Media
bio-rad.com/sizeexclusion Size Exclusion Chromatography
Ordering Information
Catalog # Description Comments Price, U.S. $
1504114 Bio-Gel P-2 Media, fine, 100 g Rapid carbohydrate, peptide, and protein desalting 295.00
1504115 Bio-Gel P-2 Media, fine, 500 g 1,090.00
1504118 Bio-Gel P-2 Media, extra fine, 100 g 295.00
1504120 Bio-Gel P-4 Media, medium, 100 g Carbohydrate and peptide separations, protein desalting 295.00
1504124 Bio-Gel P-4 Media, fine, 100 g 330.00
1504128 Bio-Gel P-4 Media, extra fine, 100 g 330.00
1504130 Bio-Gel P-6 Media, medium, 100 g Purification of proteins and polypeptides 260.00
1504134 Bio-Gel P-6 Media, fine, 100 g 295.00
1504138 Bio-Gel P-6 Media, extra fine, 100 g 295.00
1500738 Bio-Gel P-6DG Media, 100 g Rapid carbohydrate, peptide, and protein desalting; 295.00
150-0739 Bio-Gel P-6DG Media, 1 kg also available in prepacked columns and cartridges Inquire
1504140 Bio-Gel P-10 Media, medium, 100 g Purification of proteins and polypeptides 330.00
1504144 Bio-Gel P-10 Media, fine, 100 g 330.00
1504150 Bio-Gel P-30 Media, medium, 100 g 330.00
1504154 Bio-Gel P-30 Media, fine, 100 g 345.00
1504160 Bio-Gel P-60 Media, medium, 100 g 345.00
1504164 Bio-Gel P-60 Media, fine, 100 g 345.00
1504170 Bio-Gel P-100 Media, medium, 100 g 330.00
1504174 Bio-Gel P-100 Media, fine, 100 g 330.00
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 85
Chromatography Resins/Media
Size Exclusion Chromatography bio-rad.com/sizeexclusion
Ordering Information
Description Pack of 1 Price, U.S. $ Pack of 5 Price, U.S. $
Prepacked Bio-Scale Mini Cartridges
Bio-Gel P-6 Gel (Desalting), 5 ml 7324502 62.00 7324504 200.00
Bio-Gel P-6 Gel, 10 ml — 7325304 370.00
Bio-Gel P-6 Gel, 50 ml 7325312 365.00 7325314 1,485.00
Catalog # Description
Adaptor Fittings
7320111 Luer to M6 Adaptor Fittings Kit, includes luer to M6 fittings to connect 1 cartridge to an FPLC system 145.00
7320112 Luer to 10-32 Adaptor Fittings Kit, includes luer to 10-32 fittings to connect 1 cartridge to 97.00
an HPLC system
7320113 Luer to BioLogic System Fittings Kit, includes 1/4-28 female to male luer and 59.00
1/4-28 female to female luer to connect 1 cartridge to a BioLogic DuoFlow system
Bio-Gel A 1.5 m resin, ideal for purification of antibodies changing the bed volume. Bio-Gel A 1.5 m resin may
and aggregates, consists of agarose beads in which the be used at pH 4–13 and at temperatures 2–30°C.
pore size is controlled by the percentage of agarose in the The fractionation range is 10,000–1,500,000 daltons.
gel. It is compatible with all commonly used buffers and For More Information
can be used with high-salt buffers without significantly Web: bio-rad.com/biogela
Ordering Information
Catalog # Description Price, U.S. $
1510450 Bio-Gel A 1.5 m Gel, fine, 500 ml Inquire
Ordering Information
Catalog # Description Size, µm ml/dry g* Application** Price, U.S. $
1522150 Bio-Beads S-X1 Media, 100 g 40–80 7.5 1% cross-linked; for lipophilic polymers of MW 600–14,000 465.00
1522151 Bio-Beads S-X1 Media, 1 kg 40–80 7.5 1% cross-linked; for lipophilic polymers of MW 600–14,000 3 Inquire
1522750 Bio-Beads S-X3 Media, 100 g 40–80 4.75 3% cross-linked; for organic compounds of MW ≤2,000 465.00
1523350 Bio-Beads S-X8 Media, 100 g 40–80 3.1 8% cross-linked; for organic compounds of MW ≤1,000 465.00
1523650 Bio-Beads S-X12 Media, 100 g 40–80 2.5 12% cross-linked; for organic compounds of MW ≤400 540.00
* Swollen in benzene. ** MW range is for beads fully swollen in benzene.
Larger volumes and special packaging for industrial applications are available on request.
86 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Chromatography Resins/Media
bio-rad.com/HIC Hydrophobic Interaction Chromatography
Ordering Information
Description Methyl HIC Price, U.S. $ t-Butyl HIC Price, U.S. $
Macro-Prep HIC Support, 25 ml 1580080 105.00 1580090 105.00
Macro-Prep HIC Support, 100 ml 1560080 395.00 1560090 395.00
Macro-Prep HIC Support, 500 ml 1560081 Inquire 1560091 Inquire
Macro-Prep HIC Support, 5 L 156-0082 Inquire
—
Macro-Prep HIC Support, 10 L 156-0083 Inquire 156-0093 Inquire
Ordering Information
Catalog # Description Price, U.S. $
1523920 Bio-Beads SM-2 Adsorbents, 100 g 465.00
152-3922 Bio-Beads SM-2 Adsorbents, 1 kg Inquire
1523923 Bio-Beads SM-2 Adsorbents, 10 kg Inquire
1528920 Bio-Beads SM-2 Adsorbents, 25 g 125.00
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 87
Chromatography Resins/Media
Resin and Cartridge Sampler Packs, Kits, and Standards bio-rad.com/resinsampling
Sampler Packs
■■
io-Scale™
B Mini ion exchange sampler pack —
includes one 1 ml cartridge each of UNOsphere Q and S,
and Macro-Prep High Q, High S, and DEAE resins
■■
io-Scale Mini affinity sampler pack — includes
B
one 1 ml cartridge each of IMAC and Affi-Prep® Protein A
resins and one 5 ml cartridge each of DEAE Affi-Gel®
Blue and Affi-Gel Blue resins Bio-Scale Mini Cartridges
Ordering Information
Catalog # Description Price, U.S. $
Process Scale Resins/Media
1580100 Resin Sampler Pack, includes 25 ml each of Macro-Prep DEAE, Macro-Prep High Q, Macro-Prep High S, 670.00
UNOsphere Q, UNOsphere S, UNOsphere Rapid S, 5 ml of UNOsphere SuPrA, 10 g each of CHT ceramic
hydroxyapatite Types l and II, 40 µm, and 10 g of CFT ceramic fluoroapatite Type II, 40 µm
Lab Scale Resins/Media
7324650 Bio-Scale Mini Ion Exchange Sampler Pack, includes one 1 ml cartridge each of UNOsphere S, 275.00
UNOsphere Q, Macro-Prep High Q, Macro-Prep High S, Macro-Prep DEAE
7324651 Bio-Scale Mini Affinity Sampler Pack, includes one 1 ml cartridge each of IMAC and Affi-Prep Protein A, 660.00
one 5 ml cartridge each of DEAE Affi-Gel Blue and Affi-Gel Blue
88 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Chromatography Resins/Media
bio-rad.com/resinsampling Resin and Cartridge Sampler Packs, Kits, and Standards
Ordering Information
Catalog # Description Price, U.S. $
7324407 Bio-Scale Mini Apatite Purification Cartridge Kit, includes one each, 5 ml prepacked CFT ceramic 335.00
fluoroapatite Type II, 40 µm and CHT ceramic hydroxyapatite Type I, 40 µm, multimodal chromatography
media cartridges
7324408 Bio-Scale Mini mAb Purification Cartridge Kit, includes one each, 5 ml prepacked UNOsphere SUPrA 450.00
affinity media, UNOsphere Q media, and CHT ceramic hydroxyapatite Type I, 40 µm, cartridges
Chromatography Standards
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 89
Chromatography Resins/Media
Resin and Cartridge Sampler Packs, Kits, and Standards bio-rad.com/chromstandards
Standard Specifications
Description Contents MW pl For Use with
Protein Standard for Anion Equine myoglobin 17,000 6.9 UNO® Q columns; Bio-Scale™ Mini
Exchange Chromatography Conalbumin 77,000 4.9 UNOsphere™ Q cartridges;
Chicken ovalbumin 45,000 4.6 Macro-Prep™ High Q and DEAE resins;
Soybean trypsin inhibitor 21,500 4.5 UNOsphere Q resin
Protein Standard for Cation Equine myoglobin 17,000 6.9 UNO S columns; Bio-Scale Mini
Exchange Chromatography Ribonuclease A 13,500 8.7 UNOsphere S cartridges;
Cytochrome c 12,000 10.7 Macro-Prep High S and CM resins;
UNOsphere S resin
Organic Acid Analysis Sodium oxalate 134 Aminex® HPX-87H column;
Standard Sodium citrate 294 organic acid analysis kit
Sodium malate 196
Sodium succinate 270
Sodium formate 69
Sodium acetate 82
Carbohydrate Analysis Melezitose 504 Aminex HPX-87C column;
Standard Maltose 360 carbohydrate analysis kit
Glucose 180
Mannose 180
Fructose 180
Adonitol (ribitol) 152
Gel Filtration Standard Thyroglobulin 670,000 4.5 SEC columns; Bio-Gel® P resin;
Bovine g-globulin 158,000 5.1 ENrich™ SEC columns
Chicken ovalbumin 44,000 4.6
Equine myoglobin 17,000 6.9
Vitamin B12 1,350 4.5
Ordering Information
Catalog # Description Contents MW pl For Use with Price, U.S. $
1250561 Protein Standard Equine myoglobin 17,000 6.9 ENrich Q Columns; UNO Q columns; 280.00
for Anion Exchange Conalbumin 77,000 4.9 Bio-Scale Mini UNOsphere Q
Chromatography, Chicken ovalbumin 45,000 4.6 cartridges; Macro-Prep High Q and
6 vials Soybean trypsin inhibitor 21,500 4.5 DEAE media; UNOsphere Q media
1250562 Protein Standard for Equine myoglobin 17,000 6.9 ENrich S Columns; UNO S columns; 280.00
Cation Exchange Ribonuclease A 13,500 8.7 Bio-Scale Mini UNOsphere S
Chromatography, Cytochrome c 12,000 10.7 cartridges; Macro-Prep High S and
6 vials CM media; UNOsphere S media
1250586 Organic Acid Sodium oxalate 134 Aminex HPX-87H column; 165.00
Analysis Standard, Sodium citrate 294 organic acid analysis kit
6 vials Sodium malate 196
Sodium succinate 270
Sodium formate 69
Sodium acetate 82
1250585 Carbohydrate Melezitose 504 Aminex HPX-87H column; 165.00
Analysis Standard, Maltose 360 carbohydrate analysis kit
6 vials Glucose 180
Mannose 180
Fructose 180
Adonitol (ribitol) 152
1511901 Gel Filtration Thyroglobulin 670,000 4.5 ENrich SEC columns; 235.00
Standard, 6 vials Bovine g-globulin 158,000 5.1 Bio-Gel P media
Chicken ovalbumin 44,000 4.6
Equine myoglobin 17,000 6.9
Vitamin B12 1,350 4.5
90 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Chromatography Columns
bio-rad.com/columns Prepacked Chromatography Columns
Chromatography Columns
A complete range of prepacked and empty columns and accessories is available for protein and peptide separations.
Bio-Rad’s many disposable spin and gravity chromatography columns are made of chemically compatible polypropylene,
are autoclavable, and can be washed with NaOH. Our low-pressure glass Econo-Column® chromatography columns offer an
ideal combination of performance, reliability, and value. For high-resolution separations, Bio-Rad’s Bio-Scale™ MT medium-
pressure columns can be run on a variety of liquid chromatography systems and are provided prepacked with selected resins.
Learn More about the Technology
Web: bio-rad.com/tech/chrom
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 91
Chromatography Columns
Prepacked Chromatography Columns bio-rad.com/columns
Ordering Information
Column Recommended Column Flow Max. Operating Pressure
Volume, Max. Protein Dimensions
Catalog # Description ml Load, mg/column (W x L), mm psi M Pa bar Price, U.S. $
ENrich High Resolution Ion Exchange Columns
7800001 ENrich Q 5 x 50 Column 1 100 5 x 50 500 3.45 34.5 1,620.00
7800003 ENrich Q 10 x 100 Column 8 800 10 x 100 500 3.45 34.5 4,775.00
7800021 ENrich S 5 x 50 Column 1 100 5 x 50 500 3.45 34.5 1,620.00
7800023 ENrich S 10 x 100 Column 8 800 10 x 100 500 3.45 34.5 4,775.00
92 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Chromatography Columns
bio-rad.com/columns Prepacked Chromatography Columns
Ordering Information
Column Separation Column Max. Operating Pressure
Volume, Range Globular Dimensions
Catalog # Description ml Proteins, Mr (W x L), mm psi M Pa bar Price, U.S. $
ENrich High Resolution Ion Exchange Columns
7801070 ENrich SEC 70 10 x 300 Column 24 500–70,000 10 x 300 600 4.1 41 1,755.00
7801650 ENrich SEC 650 10 x 300 Column 24 5,000–650,000 10 x 300 600 4.1 41 1,755.00
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 93
Chromatography Columns
Prepacked Chromatography Columns bio-rad.com/foresight
Ordering Information
Catalog # Description Price, U.S. $
Foresight Plates*
7324714 Foresight UNOsphere Q, 20 µl 551.25
7324710 Foresight UNOsphere S, 20 µl 551.25
7324709 Foresight UNOsphere SUPrA, 20 µl Inquire
7324703 Foresight Nuvia Q, 20 µl 551.25
7324701 Foresight Nuvia S, 20 µl 551.25
7324707 Foresight Nuvia HR-S, 20 µl 540.75
7324705 Foresight Nuvia cPrime, 20 µl 551.25
7324716 Foresight CHT Type I, 40 µm 20 µl 551.25
7324718 Foresight CHT Type II, 40 µm, 20 µl 551.25
7324785 Foresight MPC Type I, 40 µm, 20 µl 551.25
continues
94 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Chromatography Columns
bio-rad.com/cartridges Prepacked Chromatography Columns
Ordering Information
Description 200 µl Price, U.S. $ 600 µl Price, U.S. $
Foresight RoboColumn Units**,***
Profinity IMAC Ni-Charged Resin 7324200 155.00 7324202 565.00
Foresight UNOsphere Q RoboColumn Units 7324819 192.94 7324820 231.53
Foresight UNOsphere S RoboColumn Units 7324813 192.94 7324814 231.53
Foresight UNOsphere SUPrA RoboColumn Units 7324834 523.69 7324835 661.50
Foresight Nuvia Q RoboColumn Units 7324804 212.78 7324805 254.68
Foresight Nuvia S RoboColumn Units 7324801 212.23 7324802 254.68
Foresight Nuvia HR-S RoboColumn Units 7324831 212.23 7324832 259.09
Foresight Nuvia cPrime RoboColumn Units 7324807 203.96 7324808 277.83
Foresight CHT Type I RoboColumn Units, 40 µm 7324822 231.53 7324823 277.83
Foresight CHT Type II RoboColumn Units, 40 µm 7324825 231.53 7324826 277.83
Foresight MPC Type I RoboColumn Units, 40 µm 7324828 231.53 7324829 264.60
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 95
Chromatography Columns
Prepacked Chromatography Columns bio-rad.com/cartridges
See Also
Ordering Information
Bottled resins:
Description 1 x 1 ml Price, U.S. $ 5 x 1 ml Price, U.S. $ 1 x 5 ml Price, U.S. $ 5 x 5 ml Price, U.S. $
pages 55–56.
BioLogic systems: Prepacked Bio-Scale Mini Cartridges
page 122. UNOsphere Q Media — — 7324100 210.00 7324102 150.00 7324104 505.00
Econo gradient UNOsphere S Media — — 7324110 210.00 7324112 150.00 7324114 505.00
pump: page 142. UNOsphere SUPrA rProtein A Media 7324200 155.00 7324201 660.00 7324202 565.00 — —
Nuvia S Media 7324420 69.00 7324421 215.00 7324422 180.00 7324423 700.00
Macro-Prep High Q Media — — 7324120 210.00 7324122 80.00 7324124 290.00
Macro-Prep High S Media — — 7324130 210.00 7324132 80.00 7324134 290.00
Macro-Prep DEAE Media — — 7324140 235.00 7324142 80.00 7324144 300.00
Affi-Prep Protein A Media — — 7324600 655.00 7324602 555.00 — —
Nuvia IMAC — — — — 7800811 160.00 7800812 600.00
Profinity IMAC Ni-Charged Resin — — 7324610 225.00 7324612 155.00 7324614 540.00
Profinity GST Resin — — 7324620 405.00 7324622 390.00 7324624 1,370.00
DEAE Affi-Gel Blue Media — — — — 7324632 165.00 7324634 560.00
Affi-Gel Blue Media — — — — 7324642 140.00 7324644 480.00
CHT Type I, 40 µm Media — — — — 7324322 155.00 7324324 615.00
CHT Type II, 40 µm Media — — — — 7324332 155.00 7324334 615.00
CFT Type II Media — — — — 7324405 155.00 7324406 615.00
Catalog # Description
Adaptor Fittings
7885010 Luer to 10-32 Adaptor Fittings Kit, includes female slip luer to female 10-32 fitting to connect male end of luer 175.00
column to NGC system
7320111 Luer to M6 Adaptor Fittings Kit, includes luer to M6 fittings to connect 1 cartridge to an FPLC system 145.00
7320112 Luer to 10-32 Adaptor Fittings Kit, includes luer to 10-32 fittings to connect 1 cartridge to an HPLC system 97.00
or NGC system
7320113 Luer to 1/4-28 Adaptor Fittings Kit, includes 1/4-28 female-to-male luer and 1/4-28 female-to-female luer to 59.00
connect 1 cartridge to a BioLogic DuoFlow system
Protein purification sampler packs offer a quick way The Bio-Scale mini mAb purification cartridge kit includes
to experiment with separation techniques when the the three most commonly used media for purification
protein to be purified has not yet been characterized. of monoclonal antibody. The kit includes a UNOsphere
The Bio-Scale Mini ion exchange sampler pack includes SUPrA™ affinity cartridge for capture, a UNOsphere Q
a 1 ml UNOsphere™ Q cartridge, 1 ml UNOsphere S cartridge for the intermediate step, and a CHT ™ type I,
cartridge, 1 ml Macro-Prep® Q cartridge, 1 ml Macro-Prep 40 µm cartridge for the polishing step.
S cartridge, and 1 ml Macro-Prep DEAE cartridge.
A list of all cartridges is included above in Ordering
The Bio-Scale Mini affinity sampler pack includes a Information for Bio-Scale Mini Cartridges. For a list of all
1 ml IMAC cartridge, 1 ml GST cartridge, 1 ml Affi-Prep® columns, see page 91.
Protein A cartridge, 5 ml DEAE Affi-Gel® Blue cartridge, For More Information
and 5 ml Affi-Gel Blue cartridge. Web: bio-rad.com/resinsampling
96 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Chromatography Columns
bio-rad.com/biospin Prepacked Chromatography Columns
Ordering Information
Catalog # Description Price, U.S. $
Bio-Scale Mini Cartridge Kits
7324407 Bio-Scale Mini Apatite Purification Cartridge Kit, includes one each of prepacked CFT 335.00
ceramic fluoroapatite Type II, 40 µm, and CHT ceramic hydroxyapatite Type I, 40 µm, mixed-mode
chromatography media cartridges, 1 x 5 ml each
7324408 io-Scale Mini mAb Purification Cartridge Kit, includes one each of UNOsphere SUPrA A and Q
B 450.00
cartridges and CHT Type I, 40 µm cartridge, 1 x 5 ml each
Bio-Scale Mini Sampler Packs
7324650 Bio-Scale Mini Ion Exchange Sampler Pack, includes one each UNOsphere Q, UNOsphere S, Macro-Prep 275.00
Q, Macro-Prep S, Macro-Prep DEAE 1 ml cartridges
7324651 Bio-Scale Mini Affinity Sampler Pack, includes one each IMAC, GST, Affi-Prep Protein A 1 ml cartridges, 660.00
and one each DEAE Affi-Gel Blue and Affi-Gel Blue 5 ml cartridges
Adaptor Fittings
7885010 Luer to 10-32 Adaptor Fittings Kit, includes female slip luer to female 10-32 fitting to connect male end of 175.00
luer column to NGC system
7320111 Luer to M6 Adaptor Fittings Kit, includes luer to M6 fittings to connect 1 cartridge to an FPLC system 145.00
7320112 Luer to 10-32 Adaptor Fittings Kit, includes luer to 10-32 fittings to connect 1 cartridge to an HPLC system 97.00
or NGC system
7320113 Luer to 1/4-28 Adaptor Fittings Kit, includes luer to 1/4-28 fittings to connect 1 cartridge to a 59.00
BioLogic DuoFlow system
Bio-Spin 6 and Micro Bio-Spin 6 columns are ideal for End cap End cap
desalting protein samples quickly using size exclusion Reservoir Reservoir
chromatography. Filled with Bio-Gel® P-6 or P-30 resin,
these columns are shipped fully hydrated in Tris buffer 3 cm
and are ready to use. These products effectively clean up 5 cm 2 cm working bed height
and remove salts, nucleotides, dye terminators, and small
molecules from protein, RNA, and DNA samples in just 3.7 cm working bed height 0.8 ml bed
volume
10 minutes. The columns are autoclavable.
1.2 ml bed volume
■■
rovide fast salt and contaminant removal in an
P Porous 30 µm
easy-to-use spin-column format polyethylene bed Luer end
support retains fitting with
■■
emove compounds <6 kD by size exclusion
R fine particles Micro Bio-Spin snap-off tip
chromatography column
Luer end fitting
■■
Accommodate up to 100 µl of sample (Bio-Spin columns) with snap-off tip
Bio-Spin column
■■
ccommodate up to 70 µl of sample
A
(Micro Bio-Spin columns) For More Information
Web: bio-rad.com/biospin
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 97
Chromatography Columns
Prepacked Chromatography Columns bio-rad.com/columns
Ordering Information
Catalog # Description Price, U.S. $
7326227 Bio-Spin 6 Columns, 25 with Tris buffer 135.00
7326228 Bio-Spin 6 Columns, 100 with Tris buffer 455.00
7326221 Micro Bio-Spin 6 Columns, 25 with Tris buffer 110.00
7326222 Micro Bio-Spin 6 Columns, 100 with Tris buffer 365.00
7326006 Bio-Spin 30 Columns, 25 with SSC buffer 155.00
7326202 Micro Bio-Spin 30 Columns, 25 with SSC buffer 110.00
7326203 Micro Bio-Spin 30 Columns, 100 with SSC buffer 365.00
Go to bio-rad.com/cartridges for current information on prepacked cartridges.
Ordering Information
Catalog # Description Price, U.S. $
1563007* Profinity eXact Mini Spin Columns, includes ten 0.6 ml spin columns, ten 2 ml capped tubes, and ten 165.00
2 ml capless tubes
* For ProteoMiner kits, see page 7.
98 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Chromatography Columns
bio-rad.com/columns Prepacked Chromatography Columns
Ordering Information
Particle Ionic
Catalog # Description Size, µm Form Application Price, U.S. $
7316211 Poly-Prep Columns, AG 1-X8 106–180 Chloride Separation of low molecular weight inorganic anions 245.00
resin, 100–200 mesh, 50
7316212 Poly-Prep Columns, AG 1-X8 45–106 Chloride For high-resolution random separations 245.00
resin, 200–400 mesh, 50
7316221 Poly-Prep Columns, AG 1-X8 45–106 Formate
Separation of low molecular weight biological compounds 245.00
resin, 200–400 mesh, 50 such as nucleotides, peptides, and carboxylic acids
7316213 Poly-Prep Columns, AG 50W-X8, 106–250 Hydrogen Separation and concentration of low molecular weight 245.00
100–200 mesh, 50 cations such as small peptides and amino acids
7316214 Poly-Prep Columns, AG 50W-X8, 63–150 Hydrogen For high-resolution random separations 250.00
200–400 mesh, 50
7311555 Poly-Prep Column Stack Cap, 50 Allows connection of pumps, reservoirs, or columns in series 43.00
7328102 Stopcock, Lock 2-Way Luer, 10 Provides control of flow through Poly-Prep columns 55.00
Ordering Information
Recommended Recommended Column Max.
Column Flow Rate, Flow Rate, Dimensions Operating
Catalog # Description Volume, ml ml/min ml/min (W x L), mm Pressure, psi Price, U.S. $
7510021 Bio-Scale CHT2-I Column 2 20 0.5–3.0 7 x 52 1,000 1,140.00
7510023 Bio-Scale CHT5-I Column 5 50 0.5–5.0 10 x 64 750 1,795.00
7510025 Bio-Scale CHT10-I Column 10 100 0.5–7.0 12 x 88 600 2,910.00
7510027 Bio-Scale CHT20-I Column 20 200 0.5–10.0 15 x 113 500 4,485.00
7510029 Top-off Resin Kit, CHT-I, 1 ml 400.00
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 99
Chromatography Columns
Prepacked Chromatography Columns bio-rad.com/columns
Ordering Information
Column Recommended Recommended Column Flow Max. Operating
Volume, Max. Protein Flow Rate, Dimensions Pressure
Catalog # Description ml Load, mg/column ml/min (W x L), mm psi M Pa bar Price, U.S. $
7200001 UNO Q1 Column 1.3 20 0.5–5.0 7 x 35 700 4.5 48 1,760.00
7200003 UNO Q6 Column 6 90 0.5–8.0 12 x 53 700 4.5 48 3,835.00
7200005 UNO Q12 Column 12 180 0.5–8.0 15 x 68 700 4.5 48 5,680.00
7200021 UNO S1 Column 1.3 20 0.5–5.0 7 x 35 700 4.5 48 1,760.00
7200023 UNO S6 Column 6 90 0.5–8.0 12 x 53 700 4.5 48 3,835.00
7200025 UNO S12 Column 12 180 0.5–8.0 15 x 68 700 4.5 48 5,680.00
UNO Replacement Columns
7200011 UNO Q1R Column 1.3 20 0.5–5.0 7 x 35 700 4.5 48 965.00
7200013 UNO Q6R Column 6 90 0.5–8.0 12 x 53 700 4.5 48 2,835.00
7200015 UNO Q12R Column 12 180 0.5–8.0 15 x 68 700 4.5 48 2,880.00
7200031 UNO SR1 Column 1.3 20 0.5–5.0 7 x 35 700 4.5 48 965.00
7200033 UNO S6R Column 6 90 0.5–8.0 12 x 53 700 4.5 48 2,705.00
7200035 UNO S12R Column 12 180 0.5–8.0 15 x 68 700 4.5 48 2,870.00
UNO Polishing PEEK Column
7200009 UNO Q Polishing Column 0.16 2 0.1–1.0 4.6 x 10 200 1.3 14 610.00
7200029 UNO S Polishing Column 0.16 2 0.1–1.0 4.6 x 10 200 1.3 14 635.00
100 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Chromatography Columns
bio-rad.com/aminex Prepacked Chromatography Columns
Ordering Information
Guard Particle Ionic Cross- pH
Catalog # Description Column* Applications Size, µm Form Linkage % Range Price, U.S. $
Carbohydrate Analysis Columns
1250143 Aminex HPX-87N 1250508 Beet sugars (USP L58) 9 Sodium 8 5–9 1,430.00
Column, 300 x 7.8 mm
1250142 Aminex HPX-87K 1250507 Molasses, corn 9 Potassium 8 5–9 1,430.00
Column, 300 x 7.8 mm
1250095 Aminex HPX-87C 1250128 High fructose corn syrup 9 Calcium 8 5–9 1,395.00
Column, 300 x 7.8 mm (USP L19)
1250094 Aminex HPX-87C 1250128 Sugar alcohols 9 Calcium 8 5–9 1,045.00
Column, 250 x 4.0 mm (USP L19)
1250098 Aminex HPX-87P 1250119 or Pentose sugars, cellulose 9 Lead 8 5–9 1,565.00
Column, 300 x 7.8 mm 1250118 hydrolysates, biofuels
(USP L34)
1250140 Aminex HPX-87H 1250129 Sugars with organic 9 Hydrogen 8 1–3 1,430.00
Column, 300 x 7.8 mm acids, biofuels (USP L17)
1250096 Aminex HPX-42C 1250128 Oligosaccharides, 25 Calcium 4 5–9 1,360.00
Column, 300 x 7.8 mm thickening agents
1250097 Fast Carbohydrate 1250119 or Glucose, galactose, 9 Lead 8 5–9 1,805.00
Column, 100 x 7.8 mm 1250118 sucrose, fructose
1250105 Fast Carbohydrate 1250119 or Glucose, galactose, 9 Lead 8 5–9 1,240.00
Column, 100 x 7.8 mm 1250118 sucrose, fructose
Organic Acid and Alcohol Columns
1250140 Aminex HPX-87H Column, 1250129 Sugars with organic 9 Hydrogen 8 1–3 1,430.00
300 x 7.8 mm acids, fermentation
monitoring, biofuels
1250100 Fast Acid Analysis Column, 1250129 Alcohol, glycol organic 9 Hydrogen 8 1–3 1,135.00
100 x 7.8 mm acid analysis
1250115 Fermentation Monitoring 1250129 Sugar, acids, alcohols 9 Hydrogen 8 1–3 1,295.00
Column, 150 x 7.8 mm
Application Kits for Food Analysis**
1250233 Carbohydrate Analysis Kit Included Carbohydrates 9 Calcium 8 5–9 1,550.00
1250234 Organic Acid Analysis Kit Included Organic acids 9 Hydrogen 8 1–3 1,530.00
continues
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 101
Chromatography Columns
Prepacked Chromatography Columns bio-rad.com/columns
Ordering Information
Ionic pH
Catalog # Description Applications Form Range Price, U.S. $
Micro-Guard Cartridges
1250118*** De-Ashing Refill Cartridges, 30 x 4.6 mm, 2 Aminex silver- and lead-form columns H+ and CO3– 6–8 340.00
1250119 Carbo-P Refill Cartridges, 30 x 4.6 mm, 2 Aminex HPX-87P column Lead 5–9 450.00
1250128 Carbo-C Refill Cartridges, 30 x 4.6 mm, 2 Aminex calcium-form columns Calcium 5–9 310.00
1250129 Cation H Refill Cartridges, 30 x 4.6 mm, 2 Aminex hydrogen-form columns Hydrogen 1–3 320.00
1250506 Anion CO3 – Cartridges, 30 x 4.6 mm, 2 Aminex silver- and lead-form columns CO3– — 510.00
1250508 Cation Na+ Cartridges, 30 x 4.6 mm, 2 Aminex HPX-87N column Sodium 5–9 365.00
Cartridge Holders and Accessories
1250131 Standard Cartridge Holder, for one 30 x 4.6 mm cartridge 695.00
1250147 Cartridge Holder Seal Replacement Kit, for #1250131 290.00
1250148 Cartridge Holder Seals, for #1250131 135.00
1250139 De-Ashing Cartridge Holder, for #1250118, holds two 3.0 x 4.6 mm cartridges in series 875.00
7800008 Fittings, 10-32 PEEK, black, 2/PK 20.00
* Supplied as 2 guard columns.
** Includes 1 column and 2 Micro-Guard cartridges; requires standard cartridge holder, catalog #1250131.
*** Requires de-ashing cartridge holder, #1250139.
Ordering Information
Catalog # Description Price, U.S. $
7322022 Econo-Pac Protein A Columns, prefilled with Affi-Gel Protein A media, 5 540.00
7322020 Econo-Pac Protein A Kit, 1 x 2 ml Affi-Gel Protein A column, 1 x 10 ml 10DG column, buffers 330.00
7322026 Econo-Pac Serum IgG Purification Column, prefilled with DEAE Affi-Gel Blue gel, 5 220.00
7322027 Econo-Pac Serum IgG Purification Kit, 5 x 5 ml DEAE Affi-Gel Blue columns, 5 x 10 ml 10DG columns, buffers 405.00
7322010 Econo-Pac 10DG Desalting Columns, 30 195.00
7380019 Econo-Pac Flow Adaptor, 1.5 cm column ID 140.00
102 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Chromatography Columns
bio-rad.com/spincolumns Empty Columns
Empty Columns
Bio-Rad’s empty columns accommodate a range of chromatography needs, including spin columns, low-pressure
columns with optional flow adaptors and jackets for temperature control, and analytical columns for medium- or
high-pressure systems.
End cap
Reservoir
5 cm
End cap
Reservoir 3.7 cm working bed height
Reservoir
3.3 cm
3 cm 2.8 cm working bed height 1.2 ml bed volume
2 cm working bed height
1.2 ml bed volume Porous 30 µm
0.8 ml bed volume polyethylene bed
support retains
fine particles
Reversible luer end
Luer end fitting fitting with snap-off tip Luer end fitting
with snap-off tip with snap-off tip
Micro Bio-Spin Column Mini Bio-Spin Column Bio-Spin Column
Ordering Information
Catalog # Description Price, U.S. $
7326008 Bio-Spin Chromatography Columns, empty, 100 170.00
7326025 Bio-Spin Chromatography Columns, empty, 1,000 1,540.00
7326204 Micro Bio-Spin Chromatography Columns, empty, 100 145.00
7326207 Mini Bio-Spin Chromatography Columns, empty, 100 Inquire
7311660 End Caps, for Micro Bio-Spin chromatography columns, 1,000 225.00
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 103
Chromatography Columns
Empty Columns bio-rad.com/spincolumns
Ordering Information
Catalog # Description Price, U.S. $
7321010 cono-Pac Chromatography Columns, empty, includes upper bed supports, end caps, tip closures, 50
E 145.00
7321011 Econo-Pac Chromatography Columns, empty, includes upper bed supports, end caps, tip closures, 500 1,330.00
Accessories
7380019 Econo-Pac Flow Adaptor, 1.5 cm column ID 140.00
7317005 Poly Column Rack, 20-place, with removable tube rack 450.00
7328102 2-Way Stopcocks, female-to-male luer, 10 55.00
7318232 Female Luer Plugs, 25, polypropylene 37.00
7311660 End Caps, for Econo-Pac columns, 1,000 225.00
104 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Chromatography Columns
bio-rad.com/econocolumns Empty Columns
Ordering Information
Catalog # Description Price, U.S. $
7311550 Poly-Prep Chromatography Columns, empty, includes end caps and tip closures, 50 88.00
7311553 Poly-Prep Chromatography Columns, empty, includes end caps and tip closures, 1,000 1,330.00
Accessories
7311555 Poly-Prep Column Stack Cap, 50 43.00
7317005 Poly Column Rack, 20-place, with removable tube rack 450.00
7328102 2-Way Stopcocks, female-to-male luer, 10 55.00
7318232 Female Luer Plugs, polypropylene, 25 37.00
7311660 End Caps, for disposable plastic columns, 1,000 225.00
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 105
Chromatography Columns
Empty Columns bio-rad.com/econocolumns
Ordering Information
Cross-Sectional Maximum
Catalog # ID, cm Length, cm Area, cm2 Volume, ml Columns/Pkg Price, U.S. $
7370507 0.5 5 0.20 1 2 57.00
7374506 0.5 5 0.20 1 4 98.00
7370512 0.5 10 0.20 2 2 57.00
7374511 0.5 10 0.20 2 4 98.00
7370517 0.5 15 0.20 3 2 57.00
7374516 0.5 15 0.20 3 4 98.00
7370522 0.5 20 0.20 4 2 57.00
7374521 0.5 20 0.20 4 4 98.00
7370707 0.7 5 0.39 2 2 57.00
7374706 0.7 5 0.39 2 4 98.00
7370712 0.7 10 0.39 4 2 57.00
7374711 0.7 10 0.39 4 4 98.00
7370717 0.7 15 0.39 6 2 57.00
7374716 0.7 15 0.39 6 4 98.00
7370722 0.7 20 0.39 8 2 57.00
7374721 0.7 20 0.39 8 4 98.00
7370732 0.7 30 0.39 12 2 57.00
7374731 0.7 30 0.39 12 4 98.00
7370752 0.7 50 0.39 20 2 57.00
7374751 0.7 50 0.39 20 4 98.00
7374006 1.0 5 0.79 4 4 98.00
7371007 1.0 5 0.79 4 2 57.00
7374011 1.0 10 0.79 8 4 98.00
7371012 1.0 10 0.79 8 2 57.00
7374021 1.0 20 0.79 16 4 98.00
7371022 1.0 20 0.79 16 2 57.00
7374031 1.0 30 0.79 24 4 98.00
7371032 1.0 30 0.79 24 2 57.00
7374051 1.0 50 0.79 40 4 98.00
7371052 1.0 50 0.79 40 2 57.00
7371091 1.0 100 0.79 79 2 140.00
7371093 1.0 120 0.79 103 2 175.00
7374150 1.5 5 1.77 9 4 150.00
7371507 1.5 5 1.77 9 2 82.00
7374151 1.5 10 1.77 18 4 150.00
7371512 1.5 10 1.77 18 2 82.00
7374156 1.5 15 1.77 27 4 150.00
7371517 1.5 15 1.77 27 2 82.00
7374152 1.5 20 1.77 35 4 150.00
7371522 1.5 20 1.77 35 2 82.00
7374153 1.5 30 1.77 53 4 150.00
7371532 1.5 30 1.77 53 2 82.00
7374155 1.5 50 1.77 89 4 170.00
7371552 1.5 50 1.77 89 2 94.00
7371576 1.5 75 1.77 124 2 125.00
7371591 1.5 100 1.77 177 2 140.00
7371593 1.5 120 1.77 230 2 175.00
7371598 1.5 170 1.77 301 2 250.00
7374250 2.5 5 4.91 25 4 150.00
7372507 2.5 5 4.91 25 2 82.00
7374251 2.5 10 4.91 49 4 150.00
7372512 2.5 10 4.91 49 2 82.00
7374252 2.5 20 4.91 98 4 150.00
7372522 2.5 20 4.91 98 2 82.00
7374253 2.5 30 4.91 147 4 170.00
7372532 2.5 30 4.91 147 2 94.00
7372551 2.5 50 4.91 246 2 125.00
continues
106 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Chromatography Columns
bio-rad.com/econocolumns Empty Columns
Ordering Information
Cross-Sectional Maximum
Catalog # ID, cm Length, cm Area, cm2 Volume, ml Columns/Pkg Price, U.S. $
7372576 2.5 75 4.91 344 2 140.00
7372591 2.5 100 4.91 491 2 195.00
7372593 2.5 120 4.91 589 2 240.00
7375011 5.0 10 19.63 196 1 140.00
7375021 5.0 20 19.63 393 1 175.00
7375031 5.0 30 19.63 589 1 210.00
7375051 5.0 50 19.63 982 1 230.00
7375071 5.0 70 19.63 1,374 1 325.00
Catalog # Description
Econo-Column Selection Packs
7376601 Econo-Column Selection Pack A, includes 7 columns, 1 each of 0.7 x 10, 20, and 30 cm; 275.00
1.5 x 30 and 50 cm; 2.5 x 20 and 50 cm
7376607 Econo-Column Selection Pack B, includes 6 columns, 1 each of 1.0 x 20, 30, and 50 cm; 240.00
1.5 x 20, 30, and 50 cm
For fittings, see low-pressure fittings on page 138.
Ordering Information
Cross-Sectional Maximum Columns/
Catalog # Description ID, cm Length, cm Area, cm2 Volume, ml Pkg Price, U.S. $
7376108 Econo-Column Jacketed Column 0.7 15 0.37 6 1 275.00
7376116 Econo-Column Jacketed Column 1.0 15 0.79 12 1 285.00
7376131 Econo-Column Jacketed Column 1.0 30 0.79 25 1 285.00
7376151 Econo-Column Jacketed Column 1.5 50 1.77 89 1 315.00
7376201 Econo-Column Open-Ended 1.0 30 0.79 25 1 420.00
Jacketed Column
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 107
Chromatography Columns
Empty Columns bio-rad.com/econocolumns
Flow adaptors significantly improve column performance by Econo-Column flow adaptors are available for 1.0, 1.5, 2.5,
eliminating head space above the gel bed and by protecting and 5.0 cm ID Econo-Column chromatography columns
the column bed from disruption during sample loading. (pages 105–106), jacketed Econo-Column chromatography
Flow adaptors improve resolution by delivering buffer and columns (page 107), and Econo-Pac® columns (page 104).
sample directly to the top of the column bed. Flow adaptors Due to wear and tear of bed supports and O-rings,
are recommended for use with any low-pressure column maintenance kits are available for standard upkeep of
connected to pumps, the BioLogic™ LP system, or other flow adaptors.
low-pressure systems.
3-position cam (1 = loose,
2 = sliding seal, 3 = fixed seal)
Ordering Information
Catalog # Description Price, U.S. $
Flow Adaptors
7380014 Flow Adaptor, 1.0 cm column ID, 1–7 cm functional length 140.00
7380015 Flow Adaptor, 1.0 cm column ID, 1–14 cm functional length 140.00
7380016 Flow Adaptor, 1.5 cm column ID, 1–14 cm functional length 140.00
7380017 Flow Adaptor, 2.5 cm column ID, 1–14 cm functional length 140.00
7380018 Flow Adaptor, 5.0 cm column ID, 1–14 cm functional length (does not include cam mechanism) 655.00
7380019 Econo-Pac Flow Adaptor, 1.5 cm column ID 140.00
Flow Adaptor Maintenance Kits
7380022 Flow Adaptor Maintenance Kit, for 5.0 cm flow adaptor, includes 2 bed supports and 2 O-rings 57.00
7380024 Flow Adaptor Maintenance Kit, for 1.0 cm adaptor with cam mechanism, includes 10 bed supports and 5 O-rings 57.00
7380025 Flow Adaptor Maintenance Kit, for 1.5 cm adaptor with cam mechanism, includes 10 bed supports and 5 O-rings 57.00
7380027 Flow Adaptor Maintenance Kit, for 2.5 cm adaptor with cam mechanism, includes 10 bed supports and 5 O-rings 57.00
108 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Chromatography Columns
bio-rad.com/econocolumns Empty Columns
Econo-Column® Funnel
Ordering Information
Catalog # Description Price, U.S. $
7310003 Econo-Column Funnels, 250 ml, 5 95.00
Ordering Information
Catalog # Description Price, U.S. $
7379112 Econo-Column Reservoir, 500 ml 210.00
7379113 Econo-Column Reservoir, 1 L 215.00
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 109
Chromatography Columns
Empty Columns bio-rad.com/econocolumns
packing, bed height adjustment, sample application, and Easy assembly for packing and cleaning
equilibration. Bio-Scale MT columns are convenient for ptimized design of sample applicator for
O
use with BioLogic™ systems or any medium- or high- Biocompatible
high-resolution chromatography
Ordering Information
Catalog # Description Column Volume, ml Pressure Limit, psi Price, U.S. $
Bio-Scale Columns*
7510081 Bio-Scale MT2 Column, 7 x 52 mm 1.9–2.3 1,000 685.00
7510083 Bio-Scale MT5 Column, 10 x 64 mm 4.6–5.7 750 715.00
7510085 Bio-Scale MT10 Column, 12 x 88 mm 9.5–11.3 600 740.00
7510087 Bio-Scale MT20 Column, 15 x 113 mm 19.4–21.9 500 840.00
110 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Medium-Pressure Chromatography Systems
bio-rad.com/NGCsystems
NGC chromatography system platforms — modular, customizable, and upgradable systems that adapt and expand to your throughput
and application needs. All NGC systems are compatible with the BioFrac™ fraction collector and the C-96 autosampler.
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 111
Medium-Pressure Chromatography Systems
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NGC™ Discover 10
NGC™ Scout 10
7880009
7880008
7880005
7880003
7880004
7880002
7880001
7880007
7880010
7880012
7880011
Product Catalog #
NGC F10 pump module 7884002 • • • • • •
NGC F100 pump module 7884003 • • • • • •
NGC mixer module 7884018 • • • • • • • • • • • •
NGC sample inject valve module 7884007 • • • • • • • • • • • •
NGC single-wavelength detector module 7884008 • • • •
NGC multi-wavelength detector module 7884009 • • • • • • • •
NGC buffer blending valve module 7884010 • • • • • • • •
NGC pH valve module (includes pH probe) 7884011 • • • • • • • •
NGC sample pump module 7884004 • • • •
NGC inlet valve module 7884006 • • • •
NGC outlet valve module 7884013 • •
NGC column switching valve module, 10 ml 7884012 • •
NGC column switching valve module, 100 ml 7884026 • •
ChromLab™ software 7886000 • • • • • • • • • • • •
NGC chromatography system and
7886500 • • • • • • • • • • • •
ChromLab software documentation
Note: All NGC systems include a touch screen and conductivity monitor and are compatible with the BioFrac fraction collector and C-96 autosampler.
112 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Medium-Pressure Chromatography Systems
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NGC Systems
Preconfigured NGC systems are designed with increasing automation and throughput in mind. All NGC systems include
either a 10 or a 100 ml/min automated dual gradient pump, a mixer module, a sample injection valve module, and a
detector with a conductivity monitor. Each preconfigured system can be further customized with additional modules to
meet your specific purification needs.
NGC Quest and NGC Quest Plus NGC Discover and Discover Pro
NGC Quest systems are designed for the all-purpose Both the NGC Discover and NGC Discover Pro
purification of proteins with automated pumps that chromatography systems are designed for higher
provide accurate gradients for high-resolution separations. throughput applications with rapid, robust automation
These systems support automated sample injection using for those that need to do method development.
fixed or dynamic sample loops.
Both NGC Discover 10 systems include the capabilities of
NGC Quest 10 and Quest 100 systems feature: the NGC Scout 10 Plus instruments but also comprise:
■■
ED-based single-wavelength light source for the
L ■■
100 ml/min integrated sample pump — supports
A
detection of proteins or nucleic acids (280 or 255 nm) contamination-free automated large volume
with high sensitivity conductivity measurements for sample application
accurate salt gradient formation ■■
Inlet valves — enable automated switching between
NGC Quest 10 Plus and Quest 100 Plus systems feature: buffers and samples, accelerating method development.
NGC supports up to 2 inlet valves for buffers and
■■
ulti-wavelength (4) detector for simultaneous detection
M
2 inlet valves for samples
(190 to 800 nm) of proteins, peptides, nucleic acids,
chromaphores, and other biomolecular complexes with
■■
column switching valve — facilitates automated
A
high sensitivity conductivity measurements for accurate column/media scouting up to 5 columns without
salt gradient formation the need to replumb. NGC supports up to 3 column
switching valves
NGC Scout™ and NGC Scout Plus ■■
sample outlet valve, available as a standard feature
A
NGC Scout systems are designed for method optimization
with the NGC Discover Pro systems — allows the
with automated pumps and valves that provide accurate
collection of up to 12 large-volume fractions and
gradients and pH for high-resolution separations suitable
enables automated Multi-D chromatography applications.
for any application.
NGC supports up to 2 outlet valves
All NGC Scout 10 and Scout 100 systems include:
Learn More about Multi-D Chromatography
■■
All features of NGC Quest systems Web: bio-rad.com/Multi-D
■■
uffer blending to automate buffer titrations for rapid
B
scouting of pH parameters
■■
radient separations at different pH values for rapid
G
method development
The NGC Scout 10 and 100 systems feature:
■■
ingle-wavelength UV (255 or 280) and conductivity
S
detector
NGC Scout 10 Plus and Scout 100 Plus systems feature:
■■
Multi-wavelength (4) UV/Vis and conductivity detector
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 113
Medium-Pressure Chromatography Systems
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Ordering Information
Catalog # Description Price, U.S. $
NGC Medium-Pressure Chromatography Systems*
7880001 NGC Quest 10 Chromatography System, includes automated 10 ml/min pumps, single-wavelength Inquire
(UV) and conductivity detection, accurate gradients, and sample injection
7880003 NGC Quest 10 Plus Chromatography System, includes automated 10 ml/min pumps, multi-wavelength Inquire
(UV/Vis) and conductivity detection, accurate gradients, and sample injection
7880002 NGC Quest 100 Chromatography System, includes automated 100 ml/min pumps, single-wavelength (UV) Inquire
and conductivity detection, accurate gradients, and sample injection
7880004 NGC Quest 100 Plus Chromatography System, includes automated 100 ml/min pumps, multi-wavelength Inquire
(UV/Vis) and conductivity detection, accurate gradients, and sample injection
7880005 NGC Scout 10 Chromatography System, includes NGC Quest 10 capability, automated 10 ml/min Inquire
pumps, pH valve and buffer blending valve for automated gradient and buffer blending
7880007 NGC Scout 10 Plus Chromatography System, includes NGC Quest 10 Plus capability, automated 10 ml/min Inquire
pumps, pH valve and buffer blending valve for automated gradient and buffer blending
7880006 NGC Scout 100 Chromatography System, includes NGC Quest 100 capability, automated 100 ml/min Inquire
pumps, pH valve and buffer blending valve for automated gradient and buffer blending
7880008 NGC Scout 100 Plus Chromatography System, includes NGC Quest 100 Plus capability, automated Inquire
100 ml/min pumps, pH valve and buffer blending valve for automated gradient and buffer blending
7880009 NGC Discover 10 Chromatography System, includes NGC Scout 10 Plus capability, automated 10 ml/min Inquire
pumps, sample pump for large sample injection, column switching valve (up to 5 columns), and buffer inlet valve
7880010 NGC Discover 100 Chromatography System, includes NGC Scout 100 Plus capability, automated 100 ml/min Inquire
pumps, sample pump for large sample injection, column switching valve (up to 5 columns), and buffer inlet valve
7880011 NGC Discover Pro 10 Chromatography System, includes NGC Scout 10 Plus capability, Inquire
automated 10 ml/min pumps, sample pump for large sample injection, column switching valve
(up to 5 columns), buffer inlet valve, and outlet valve
7880012 NGC Discover Pro 100 Chromatography System, includes NGC Scout 100 Plus capability, Inquire
automated 100 ml/min pumps, sample pump for large sample injection, column switching valve
(up to 5 columns), buffer inlet valve, and outlet valve
* All NGC systems include ChromLab software.
Multi-D Chromatography
12001088 NGC Multi-D Chromatography Starter Kit Inquire
Module Overview
The NGC family of medium-pressure chromatography systems offers flexible system configurations that enable upgrades
and reconfiguration of the modules to fit multi-user needs, applications, and laboratory space requirements. All NGC
systems can be individually customized using NGC plug-and-play modules that are user installable. Systems can be easily
reconfigured and seamlessly upgraded with increased functionality such as higher flow rates (10 ml/min or 100 ml/min),
sophisticated detection capabilities, pH monitoring, automated buffer selection, multicolumn connection, and buffer blending.
114 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Medium-Pressure Chromatography Systems
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NGC F10 and F100 System Pump Modules The outlet valve also enables automated tandem/2-D and
The F10 and F100 pumps produce buffer gradient Multi-D chromatography applications by directing peaks
solutions. Flow rates on all pumps can be automatically of interest onto the next column.
controlled to avoid overpressure. NGC instruments can
have up to three high-precision pumps: two system
gradient pumps (Pump A and Pump B) and one
sample pump.
NGC Sample Pump 100 Module
The NGC sample pump 100 module enables both the
injection of large sample volumes and accurate and
consistent multiple loadings from a single sample stock. NGC Sample Pump
This dedicated pump eliminates the risk of contaminating Module
system pumps, allowing for automated loading of large
sample volumes directly to the column. The sample pump
also includes an integrated pressure sensor that protects
the column and resin from overpressure. Specifications for System and Sample Pump Modules
F10 Pump
NGC Sample Inject Valve Module
Flow rates 0.001–10 ml/min (normal range);
The NGC sample inject valve enables the system to load
0.002–20 ml/min (column packing flow)
a specific predetermined volume of sample onto a column
Max pressure limit 3,650 psi (252 bar, 25.2 MPa)
with a maximum operating pressure of 3,650 psi. It is
also compatible with capillary loops and sample pumps, F100 Pump
eliminating the need to replumb. The C-96 autosampler Flow rate setting 0.01–100 ml/min (normal range);
0.02–200 ml/min (column packing flow)
can also be plumbed through the inject valve to enable
Max pressure limit 1,450 psi (100 bar, 10 MPa)
multiple small volume sample injections.
Sample Pump 100
NGC Single-/Multi-Wavelength Detector Modules Flow rate up to 100 ml/min
The NGC single UV and multi UV/Vis detector modules Max pressure limit 1,450 psi (100 bar, 10 MPa)
are combined with an integrated conductivity monitor to
measure buffer conductivity or salt gradients.
The single-wavelength UV detector contains an LED UV
lamp and monitors UV absorbance of proteins or nucleic
acids at 280 or 255 nm, one wavelength at a time.
The multi-wavelength UV/Vis detector adds flexibility to
the chromatography system, enabling the simultaneous
monitoring of four wavelengths (UV and Vis) for greater
sensitivity and detection of proteins, peptides, nucleic
acids, and chromaphores such as hemoglobin. NGC Multi-Wavelength
Detector Module
NGC Outlet Valve Module
Use the NGC outlet valve either as a stand-alone unit
or in conjunction with the BioFrac™ Fraction Collector
(#7410002).
As a single, stand-alone unit, the outlet valve is capable Specifications for Single-/Multi-Wavelength Detector Modules
of collecting up to 12 large-volume fractions such as UV Monitor
flowthrough or waste. Two outlet valves may be daisy- Single-wavelength range 255 or 280 nm
chained to collect up to 23 large-volume fractions, Multi-wavelength (up to 4) range 190–800 nm
including waste. Conductivity Monitor
Conductivity range 0.01–999.99 mS/cm
When a BioFrac fraction collector is connected the
collection sequence can be programmed to collect
smaller-volume fractions in the BioFrac fraction collector
and larger-volume fractions via the outlet valves, providing
full flexibility.
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Medium-Pressure Chromatography Systems
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116 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Medium-Pressure Chromatography Systems
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Ordering Information
Catalog # Description Price, U.S. $
NGC Medium-Pressure Chromatography System Modules and Accessories
System Pumps
7884002 GC F10 Pump Module, includes 10 ml/min system pump kit for creating buffer gradients; can be used
N 5,450.00
in conjunction with buffer blending valve to generate flow rates up to 20 ml/min; kit includes necessary
tubing and fittings
7884003 NGC F100 Pump Module, includes 100 ml/min system pump kit for creating buffer gradients; can 7,500.00
be used in conjunction with buffer blending valve to generate flow rates up to 200 ml/min; kit includes
necessary tubing and fittings
Sample Pump
7884004 NGC Sample Pump 100 Module, includes 100 ml/min sample pump kit for automated large volume sample 5,950.00
application via the sample inject valve; kit includes necessary tubing and fittings
Detectors
7884008 NGC Single-Wavelength Detector Module, for detection of nucleotides and proteins at 255 or 280 nm and 4,950.00
generation of salt gradients; UV/conductivity detector kit includes necessary tubing and fittings
7884009 NGC Multi-Wavelength Detector Module, for simultaneous wavelength (4) monitoring of elution fractions Inquire
190–800 nm and generation of salt gradients; UV/Vis, conductivity detector kit includes necessary tubing
and fittings
7885024 NGC UV Flow Cell, 5 mm, UV flow cell, standard with all systems. Fits single-wavelength UV and 580.00
multi-wavelength UV/Vis detectors in both F10 and F100 systems
7885023 NGC UV Flow Cell, 10 mm, analytical UV flow cell. Fits single-wavelength UV and multi-wavelength UV/Vis 580.00
detectors in F10 systems. Ideal for use at lower flow rates
7885022 NGC UV Flow Cell, 2 mm, preparative UV flow cell. Fits single-wavelength UV and multi-wavelength UV/Vis 1,450.00
detectors in F100 systems. Ideal for use at high flow rates
7500230 Backpressure Regulator, 40 psi, restricts flow and creates appropriate backpressure to prevent bubble 160.00
spiking in UV detectors
Detector Lamp Replacements
7885000 NGC LED Lamp Replacement, includes 255/280 nm LED lamp replacement for single-wavelength detectors 1,260.00
7885001 NGC Deuterium Lamp Replacement, includes deuterium lamp replacement (UV) for multi-wavelength detectors 2,075.00
7885002 NGC Tungsten Lamp Replacement, includes tungsten lamp replacement (Vis) for multi-wavelength detectors 630.00
7885056 NGC Replacement Conductivity Monitor, includes replacement conductivity monitor Inquire
pH Valve
7884011 NGC pH Valve Module, for accurate inline pH measurement; includes pH probe, tubing, and fittings 4,100.00
7885026 NGC pH Probe (Ag/AgCl), for use with the pH valve for accurate pH monitoring (pH 0–14) during purifications 500.00
7885027 NGC Blank pH Probe, pH valve cap for use with the pH valve when the probe is removed Inquire
Mixer
7884018 GC Mixer Module, for use with all NGC systems; additional mixer base or barrels (ordered separately) can be
N 2,100.00
extended with 2, 5, and 12 ml barrels for efficient gradient mixing at higher flow rates
7884019 NGC F100 Mixer, 750 µl base and top assembly, included with all 100 ml/min NGC systems 625.00
7884020 NGC F10 Mixer, 263 µl base and top assembly, included with all 10 ml/min NGC systems 210.00
7884021 NGC F10 Mixer Barrel Kit, 750 µl extension barrel for F10 263 µl mixer (part of Scout 10 and Discover 10 series) 99.00
7884022 NGC F10 Mixer Barrel Kit, 2 ml extension barrel for F10 263 µl mixer (optional) 99.00
7884028 NGC F100 Mixer Barrel Kit, 2 ml extension barrel for F100 750 µl mixer body, part of Scout 100, Discover 100 series 250.00
7884023 NGC F100 Mixer Barrel Kit, 5 ml extension barrel for F100 750 µl mixer (optional) 250.00
7884024 NGC F100 Mixer Barrel Kit, 12 ml extension barrel for F100 750 µl mixer (optional) 450.00
Valves
7884006 GC Inlet Valve Module, for automated switching between multiple buffers and samples during methods, kit
N 3,200.00
includes the necessary tubing and fittings
7884007 NGC Sample Inject Valve Module, for manual sample application of small volume samples via sample loops or 2,600.00
large volume samples using a sample pump; kit includes necessary tubing, fittings, and sample injection port;
valve can also be used with DynaLoops, sample pumps, and the C-96 autosampler
7884010 NGC Buffer Blending Valve Module, for online buffer preparation and generating pH gradients for quick pH 5,500.00
scouting; kit includes the necessary tubing and fittings; can also double system pump flow rate
7884012 NGC Column Switching Valve Module, 10 ml, for use with F10 systems and multiple columns for quick column 5,500.00
scouting and reverse flow, holds 5 columns; kit includes the necessary tubing and fittings to accommodate the most
common column types
7884013 NGC Outlet Valve Module, for collection of up to 12 large volume fractions, including necessary tubing and fittings 3,000.00
7884026 GC Column Switching Valve Module, 100 ml, for use with F100 systems and multiple columns for quick column
N 5,500.00
scouting and reverse flow, holds 5 columns; kit includes the necessary tubing and fittings to accommodate the most
common column types
continues
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Medium-Pressure Chromatography Systems
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Ordering Information
Catalog # Description Price, U.S. $
Maintenance Kits
7885003 Pump Maintenance Kit, 10 ml, for regular maintenance of NGC F10 pumps, includes seals and check valves 950.00
7885004 Pump Maintenance Kit, 100 ml, for regular maintenance of NGC F100 pumps, includes seals and check valves 1,070.00
7885005 Pump Maintenance Kit, Sample pump, 100 ml, for regular maintenance of NGC sample pump, 3,375.00
includes seals and check valves
Air Sensor
7885017 GC Air Sensor Module, for detection of end of buffer and sample, protects against air entering pumps and
N 3,200.00
columns, supports up to 4 air sensors (large- and small-bore); kit includes 2 large-bore air sensors
7885018 NGC Air Sensor Extension Module, connects to the base air sensor module to support 4 additional air sensors 820.00
(optional, does not include any air sensors)
7885020 NGC Air Sensor, small, includes air sensor; enables exclusion of air from system and columns. 1,600.00
Detects air in small-diameter PEEK tubing
7885021 NGC Air Sensor, large, includes air sensor; enables exclusion of air from system and columns. 1,600.00
Detects air in large-diameter PTFE tubing
Fraction Collector and Autosampler
7410002 BioFrac Fraction Collector, 100/240 V, fraction collector compatible with all NGC systems, includes 5,415.00
power cord, rack set F1 (2 x flatpack, 13 mm), BioFrac diverter valve, fittings kit
7884025 NGC Communication Adaptor, enables communication between Bio-Rad devices such as the BioFrac 395.00
fraction collector and the NGC system
7605011 C-96 Autosampler without Cooling, 110/240 V, compatible with all NGC systems, includes standard 84+3 vial Inquire
tray (1.5 and 10 ml), control cable set to connect with BioLogic DuoFlow system, 1 ml syringe, 2 ml sample loop;
also includes #7605014, #7605026, and #7600604
7605010 C-96 Autosampler with Cooling, 110/240 V, compatible with all NGC systems, includes standard 84+3 vial tray Inquire
(1.5 and 10 ml), control cable set to connect with BioLogic DuoFlow system, 1 ml syringe, 2 ml sample loop; also
includes #7605014, #7605026, and #7600604
7884016 NGC Signal Import Module, enables analog-to-digital signal conversion and connection to devices such 1,500.00
as the C-96 autosampler, includes cable for connecting to NGC system (#7885013) and external detectors
NGC Accessories
7885038 NGC Column Holder, column holder for use with NGC systems, pkg of 1 150.00
7885039 NGC Cartridge Holder, universal cartridge holder, holds 1–10 ml cartridges 68.00
7885041 NGC Sample/Wash Tube Holder, holds two 50 ml Falcon tubes, for use with NGC systems (tubes not included) 68.00
7885042 NGC Tubing Retainers (small), holds small PEEK tubing in an organized manner, pkg of 3 magnetic retainers 160.00
7885035 NGC Tubing Retainers (large), holds larger PTFE tubing in an organized manner, pkg of 3 magnetic retainers 160.00
7885031 NGC Inline Filter Kit, includes buffer inline filter kit, filters particulates from buffer and prevents clogging of columns Inquire
7884017 NGC Fittings Kit, includes PEEK and Tefzel nuts, ferrules, unions, plugs, tubing cutter, fittings tightener, and luer 950.00
syringe, for use with NGC systems
Additional Options
7884000 NGC Expansion Bay, tier 3, expands base system to 3-tier system for use with additional modules 2,500.00
7884001 NGC Expansion Bay, tier 4, expands system to 4-tier system for use with additional modules 2,500.00
7885014 NGC Buffer Tray, holds up to 8 buffer bottles. Includes drain port to prevent flooding in the event of a leak 160.00
7885016 NGC Drip Tray, for use with NGC systems 160.00
7885040 NGC Touch Screen Stand, used to mount touch screen outside of coldbox or -room while tethered 555.00
to the system, enables convenient system operation from the outside
7885060 Touch Screen Bracket, for use with standard third-party monitor stands, used to mount touch screen 265.00
outside the coldroom or -box while tethered to system, includes cable
7884015 Computer, for use with NGC systems, includes Intel Core i7 3.4 GHz (quad core), 4 GB RAM, 1,950.00
1 x 250 GB hard drive, CD/DVD drive, Microsoft Windows 7 Professional 64-bit edition
7884027 Monitor, for use with NGC system computer 375.00
7880020IQOQ NGC IQ/OQ, NGC Quest/Quest Plus IQ/OQ (with BioFrac and air sensor), Installation Qualification and 6,999.00
Operational Qualification (IQ/OQ) performed by field service engineers on location at the customer’s site,
duration: 1 day
7880030IQOQ NGC Scout/Discover/Discover Pro IQ/OQ (with BioFrac and air sensor), Installation Qualification and Inquire
Operational Qualification (IQ/OQ) performed by field service engineers on location at the customer’s site,
duration: 2–3 days
118 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Medium-Pressure Chromatography Systems
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ChromLab™ Software
ChromLab is available in standard format (free) as an acquisition, each component of the fluidics scheme can be
upgrade for existing ChromLab software users. It contains controlled to reach optimized conditions. In both manual and
enhancements to existing ChromLab features and a linear automated method-based runs, the software highlights the
flow rate calculator. real-time fluidics path and module status to ensure accuracy.
Graphical Instrument Setup and Control The instrument control interface is touch-screen
ChromLab software instrument controls are designed around optimized, allowing flexibility without the need for a
a novel fluidics scheme interface that can be customized to computer adjacent to the system, thus minimizing
exact hardware configurations. ChromLab enables easy setup the total footprint.
with its novel Point-to-Plumb™ feature. During manual data
Fluidics Scheme
Real-time status
display of flow
path devices
Graphical manual
control panels
allow complete,
accessible manual
control of the
Complete system status and control is available via the graphical fluidics scheme. system
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 119
Medium-Pressure Chromatography Systems
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See Also Streamlined Method Development Methods are created by drag-and-drop functional
Multi-D ChromLab software includes templates for common phases. Grouping parameters into intuitive phases puts
chromatography chromatographic techniques and a column database for the focus on separation workflow instead of hardware
applications:
page 113. maximum versatility. Select a column from the column controls. The interactive gradient graph enables
database. The software automatically adjusts optimal flow visualization of the protocol and quick click-and-drag
rate and pressure parameters for the selected column. experimental adjustments.
Phase-based
method editing
that reflect common automated purification scenarios. chromatography system for lab-scale protein purification
These templates may be edited to fit your research and ■■
Method development to identify best methods
reduce hands-on time. ■■
eal-time monitoring of proteins, peptides, nucleic acids,
R
Features and Benefits of ChromLab Software and chromophores
Touch screen allows the same level of flexibility without
■■
■■
Chromatogram comparison and peak analysis
the need for a computer adjacent to the system, ■■
dvanced integration parameters enable manual integration
A
minimizing the total footprint for an operational system
functions and peak addition or removal for fine-tuning data
■■
ustomizable chromatogram layouts with a variety of
C ■■
rganized data and easy navigation with intuitive
O
viewing and data analysis options
grouped table layouts
■■
apid creation of trace comparisons for easy analysis
R ■■
Multi-instrument control
across multiple purification runs
■■
Centralized database for easy data storage and analysis
■■
ingle-click peak detection and integration for
S
quicker results For More Information
Web: bio-rad.com/chromlab
■■
dvanced integration parameters to enable manual
A
integration functions and peak addition or removal for Learn More about Multi-D Chromatography
fine-tuning your data Web: bio-rad.com/Multi-D
■■
Intuitive, grouped data tabular layouts that organize data
for easy navigation
Ordering Information
Catalog # Description Price, U.S. $
7886000 hromLab Software CD, single software platform compatible with all NGC systems, includes integrated system
C 2,500.00
setup controls, method development, data acquisition, and analysis. Compatible with Windows 7–10 64 bit
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Ordering Information
Catalog # Description Price, U.S. $
17000099 hromLab 4.0 User Management License, 1 seat
C Inquire
17000098 ChromLab 4.0 User Management License, 3 seats Inquire
17000097 ChromLab 4.0 User Management License, 5 seats Inquire
Ordering Information
Catalog # Description Price, U.S. $
7886001 hromLab Security Edition (SE Software) CD, single software platform compatible with all NGC systems,
C 7,000.00
includes integrated system setup controls, method development, data acquisition, and analysis. Security
edition also provides features in accordance with 21 CFR Part 11 requirements. 1 license
7886003 ChromLab Security Edition (SE Software) CD, 3 licenses Inquire
7886005 ChromLab Security Edition (SE Software) CD, 5 licenses Inquire
Ordering Information
Catalog # Description Price, U.S. $
7880020IQOQ GC Quest/Quest Plus IQ/OQ (with BioFrac and air sensor), Installation Qualification and Operational
N 6,999.00
Qualification (IQ/OQ) performed by field service engineers on location at the customer’s site, duration: 1 day
7880030IQOQ NGC Scout/Discover/Discover Pro IQ/OQ (with BioFrac and air sensor), duration: 2–3 days Inquire
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Medium-Pressure Chromatography Systems
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Bio-Rad offers a complete line of laboratory-scale for protein separations paying close attention to the selection
chromatography instruments that are flexible, upgradable, of materials, fraction collection, and the programming
and easy to use. These instruments are specifically designed flexibility required when working with biological samples.
2 1
3
15
7
13 6
16
5
12
14
122 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Medium-Pressure Chromatography Systems
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The BioLogic DuoFlow family of chromatography systems Upgradable Systems Add Capability
offers flexibility with multiple system configurations, many as Research Needs Change
optional upgrades, and a common software platform that BioLogic DuoFlow modular components allow the
is intuitive and easy to follow. These systems can be used system to meet both laboratory space and application
on the laboratory bench or in a coldroom and are suitable requirements. As requirements change, systems may
for analytical and preparative chromatography. be easily reconfigured and seamlessly upgraded with
increased functionality such as higher flow rates,
A Dell PC controller enables easy communication with the
sophisticated detection capabilities, pH monitoring,
workstation and peripheral devices via an external USB
column scouting, and buffer blending. The BioLogic
bitbus communicator. The controller includes the Windows
DuoFlow system selection guide on the previous page
7 operating system, application software, keyboard,
lists the systems, their functions, and available options.
mouse, and high-resolution flat-panel monitor.
For More Information
Web: bio-rad.com/DFmediumpressure
Request or download bulletins: 2687 and 5369
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Valve S Maximizer
UV/Vis QuadTec
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Collecto
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BioFrac
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Convers
214 nm
BioLog
BioLog
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F40 Pu
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BioLogic DuoFlow System Options
Page numbers for components 124 124 132 136 125 125 125 125
BioLogic DuoFlow 10 system • ° ° ° ° • • °
0.01–10 ml/min flow rate, 3,500 psi
BioLogic DuoFlow 40 system ° • ° ° ° • • °
0.5–40 ml/min* flow rate, 1,000 psi
• Included as standard; ° option or upgrade. * To double the flow rate, use the BioLogic Maximizer valve system.
Ordering Information
Catalog # Description Price, U.S. $
BioLogic DuoFlow Systems*
7600037 BioLogic DuoFlow 10 System, 100/120 V, includes Dell controller and monitor, USB bitbus Inquire
communicator, F10 workstation, MX-1 mixer, 3-tray rack, AVR7-3 sample inject valve, fittings kit,
UV detector with 5 mm flow cell and 254/280 nm filters, conductivity monitor, starter kit
7600036 BioLogic DuoFlow 10 System, 100/120 V, for Japan and Korea only, does not include monitor Inquire
7600038 BioLogic DuoFlow 10 System, 220/240 V, does not include monitor Inquire
7604037 BioLogic DuoFlow 40 System, 100/120 V, same as #7600037 with F40 workstation replacing F10 workstation Inquire
7604036 BioLogic DuoFlow 40 System, 100/120 V, for Japan and Korea only, does not include monitor Inquire
7604038 BioLogic DuoFlow 40 System, 220/240 V, does not include monitor Inquire
7600047 BioLogic DuoFlow 10 System with BioFrac Fraction Collector, 100/120 V, includes Dell controller Inquire
and monitor, USB bitbus communicator, F10 workstation, MX-1 mixer, 3-tray rack, AVR7-3 sample
inject valve, fittings kit, UV detector with 5 mm flow cell and 254/280 nm filters, conductivity monitor,
starter kit, diverter valve, two F1 racks
7600046 BioLogic DuoFlow 10 System with BioFrac Fraction Collector, 100/120 V, for Japan and Korea only, Inquire
does not include monitor
7600048 BioLogic DuoFlow 10 System with BioFrac Fraction Collector, 220/240 V, does not include monitor Inquire
7604047 BioLogic DuoFlow 40 System with BioFrac Fraction Collector, 100/120 V, same as #7600047 with Inquire
F40 workstation replacing F10 workstation
7604046 BioLogic DuoFlow 40 System with BioFrac Fraction Collector, 100/120 V, for Japan and Korea only, Inquire
does not include monitor
7610001 BioLogic DuoFlow 10 Core with BioFrac Fraction Collector, 100/120 V Inquire
7610002 BioLogic DuoFlow 10 Core with BioFrac Fraction Collector, 220/240 V Inquire
* The 10 system includes an F10 workstation. The 40 system includes an F40 workstation.
Note: Additional detectors, valves, and accessories are optional and available.
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Medium-Pressure Chromatography Systems
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Ordering Information
Catalog # Description Price, U.S. $
BioLogic DuoFlow Workstations
7600150 BioLogic DuoFlow F10 Workstation Inquire
7600140 BioLogic DuoFlow F40 Workstation Inquire
Pump Kits
7600110 10 Pump Kit, converts F40 workstation to F10 pumps to enable flow rates as low as 0.01 ml/min;
F 6,020.00
includes 2 fully assembled pump heads, 4 piston assemblies, F10 tubing kit, tools
7600180 F40 Pump Kit, expands pumping capabilities to 40 ml/min; includes 2 fully assembled pump heads, 3,650.00
4 piston assemblies, mixer barrel extender, 2 mm UV flow cell, F40 tubing kit, tools
Mixers
7600170 MX-1 Mixer, includes mixer body (263 µl) and standard mixer barrel for total volume of 750 µl 1,020.00
7600171 Mixer Barrel Extender, for total volume of 2 ml; one included in the F40 pump kit (#7600180) 135.00
7602010 BioLogic Maximizer Mixer, includes 750 µl mixer body, 5 ml and 12 ml mixer barrel extenders, 1,825.00
5 O-rings, stirbar, installation screws
7602005 BioLogic Maximizer Mixer Barrel Extender, 5 ml 145.00
7602012 BioLogic Maximizer Mixer Barrel Extender, 12 ml 420.00
BioLogic DuoFlow Workstation Accessories
7600164 F10 Pump Maintenance Kit, to service one F10 pump, includes 2 piston seals, 4 check valves, 410.00
seal removal tool, 2 O-rings
7600161 F10 Piston Seals, 2, includes seal tool, to service one F10 pump 120.00
7600162 F10 Piston Kit, 2 pistons, to service one F10 pump 360.00
7600184 F40 Pump Maintenance Kit, to service one F40 pump, includes 2 piston seals, 4 check valves, 410.00
seal removal tool, 2 O-rings
7600172 F40 Piston Seals, 2, includes seal tool, to service one F40 pump 115.00
7600173 F40 Piston Kit, 2 pistons, to service one F40 pump 470.00
7500162 Check Valve, 1 (4 required per pump) 74.00
7500703 Inline Filter Kit, includes 1 filter unit, 2 replacement frits 94.00
7500230 40 psi Backpressure Regulator 160.00
7600135 BioLogic System Starter Kit 235.00
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Medium-Pressure Chromatography Systems
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Ordering Information
Catalog # Description Price, U.S. $
7500200 ioLogic DuoFlow Detector Kit, includes UV optics module and conductivity monitor, 5 mm analytical flow cell
B 4,655.00
7500202 UV Optics Module, 5 mm analytical flow cell 4,540.00
7500240 Conductivity Monitor 475.00
7500210 Flow Cell, preparative, 2 mm (30 ml) pathlength 1,375.00
7500212 Flow Cell, analytical, 5 mm (16 ml) pathlength 1,130.00
7500216 Mercury Lamp, for use at all wavelengths except 214 nm 760.00
7500220 Detector Filters, 254 and 280 nm 565.00
7500224 Detector Filter, 405 nm 400.00
7500225 Detector Filter, 436 nm 420.00
7500214 214 nm Conversion Kit, for detectors with serial #362BRXXXX, includes zinc lamp, housing, 2,570.00
and 214 nm filter for peptide detection
7500217 Zinc Lamp, for 214 nm detection 1,150.00
7500221 Detector Filter, 214 nm, requires zinc lamp 560.00
7601300 BioLogic QuadTec Detector Kit, includes BioLogic QuadTec detector with 3 mm PEEK flow cell, Inquire
instrument control module (ICM), system cables 25, 26, and 17 (BioLogic QuadTec RS-232, ICM power,
and bus communication), U.S. power cord, 40 psi backpressure regulator
7601330 Deuterium Lamp, replacement 900.00
7601332 Halogen Lamp, replacement 225.00
7601331 Halogen Lamp, with holder for first-time halogen lamp change 380.00
7601306 Standard Flow Cell, 3 mm pathlength (2 µl) 1,220.00
7601406 High-Speed Flow Cell, 2 mm pathlength, flow rate to 80 ml/min with fittings 2,125.00
7601311 Long Fingertight Fittings, 10-32 x 1.03", 4 51.00
7601320 Instrument Control Module (ICM) Kit, includes ICM power cable and cable 17 760.00
7500650 System Cable 17, bus communication cable, 1.2 m (4') 64.00
7601307 System Cable 25 (BioLogic QuadTec RS-232), connects BioLogic QuadTec detector to ICM 57.00
7601321 System Cable 26 (ICM Power), connects to 12 V power on BioLogic DuoFlow workstation 67.00
7500230 40 psi Backpressure Regulator 160.00
7602046 H Tubing Kit, includes orange and green PEEK 1/4–28 prefitted tubing lengths for connecting the
p 79.00
pH flow cell to the chromatography system
Signal Import Module (SIM)
7500502 Signal Import Module, includes 4' communication cable (system cable 17) 930.00
7602034 Universal AC/DC Inline Adaptor for USB Bitbus Device, required when connecting pH monitor or 66.00
other external detector through SIM module on BioLogic DuoFlow systems
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Medium-Pressure Chromatography Systems
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126 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Medium-Pressure Chromatography Systems
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Ordering Information
Catalog # Description Price, U.S. $
BioLogic DuoFlow Valves and Rebuild Kits
7600406 AVR7-3 Automated Sample Injection Valve, 7-port, 3-position high-pressure valve, 3,500 psi (233 bar) limit 3,045.00
7600401 AVR7-3 Valve Rebuild Kit 480.00
7600408 AVR9-8 Stream-Select Valve, 9-port, 8-position high-pressure valve, 3,500 psi (233 bar) limit 2,390.00
7600403 AVR9-8 Valve Rebuild Kit 480.00
7600410 SVT3-2 Diverter Valve, 3-port, 2-position solenoid valve, 30 psi (2 bar) limit 635.00
7600411 SVT3-2 Valve Rebuild Kit 115.00
7500415 SV5-4 Select Valve, 5-port, 4-position solenoid valve, 30 psi (2 bar) limit 1,075.00
BioLogic DuoFlow Fittings Kit
7600550 BioLogic DuoFlow Fittings Kit 535.00
BioLogic Single-Injection Sample Loops, Kits, and Accessories
7500471 Sample Injection Port, for use with AVR7-3 automated sample injection valve 34.00
1250224 Injection Needle, 22 gauge, blunt 69.00
7500490 Small-Volume Sample Loop Kit, includes 100, 250, and 500 µl PEEK loops 225.00
7500491 Large-Volume Sample Loop Kit, includes 1, 2, and 5 ml PEEK loops 300.00
7500482 25 µl Tefzel Sample Loop 59.00
7500483 50 µl Tefzel Sample Loop 65.00
BioLogic Single-Injection Sample Loops, Kits, and Accessories
7500492 100 µl PEEK Sample Loop 74.00
7500493 250 µl PEEK Sample Loop 74.00
7500494 500 µl PEEK Sample Loop 74.00
7500495 1 ml PEEK Sample Loop 74.00
7500496 2 ml PEEK Sample Loop 99.00
7500497 5 ml PEEK Sample Loop 135.00
BioLogic Dynamic (Sliding Piston) Sample Loops, Kits, and Seal Replacement
7500451 DynaLoop 25 Kit, includes 25 ml DynaLoop sliding piston loop, DynaLoop parts kit 1,345.00
7500452 DynaLoop 90 Kit, includes 90 ml DynaLoop sliding piston loop, DynaLoop parts kit 1,685.00
7500450 DynaLoop Parts Kit, includes 4 end cap O-rings, 1 sliding seal O-ring, 1 filter, 4 nut fittings, four 1/8" ferrules, 355.00
five 1/4–28 nuts and ferrules, 10' of 1/8" tubing
7500475 DynaLoop 25 Sample Loop, 25 ml, replacement 1,120.00
7500476 DynaLoop 90 Sample Loop, 90 ml, replacement 2,190.00
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 127
Medium-Pressure Chromatography Systems
bio-rad.com/DFmedpressurecomponents
Ordering Information
Catalog # Description Price, U.S. $
7602050 ioLogic DuoFlow Software Version 5.3 Upgrade, upgrades existing BioLogic DuoFlow version 5.0 systems,
B 1,995.00
includes version 5.3 software CD
Ordering Information
Catalog # Description Price, U.S. $
7500111 EZLogic Integration Software Package 2,615.00
128 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Medium-Pressure Chromatography Systems
bio-rad.com/DFmediumpressurecomponents
Bio-Rad offers qualification protocols and services for the IQ service verifies that the BioLogic DuoFlow
BioLogic DuoFlow chromatography systems and their chromatography system is properly installed. OQ service
peripheral components, including the BioLogic Maximizer™ tests the performance of the installed system and provides
valve system for buffer blending, the BioLogic QuadTec™ a record that confirms critical functions and safety features.
UV/Vis detector, and the BioFrac™ fraction collector. For more information on IQ/OQ for the BioLogic DuoFlow
Bio-Rad’s IQ/OQ protocols are designed to help comply chromatography system, contact your local Bio-Rad
with U.S. FDA regulatory requirements. Procedures are sales representative.
performed by factory-trained and certified technicians
using instruments and reagents traceable to NIST standards.
BioLogic™ Rack
Bar
Clamps
Cable Organizers
BioLogic rack with optional expansion kit.
Dimensions are 34 x 41 x 64 cm (W x D x H).
Ordering Information
Catalog # Description Price, U.S. $
7500251 ioLogic Rack, includes rack tray, 8 sleeves, 2 short vertical bars, 2 long vertical bars, column clamp set,
B 1,385.00
5 bar clamps, 4 cable organizers
7500268 BioLogic Rack Expansion Kit, includes 2 rack trays, 2 long vertical bars, 16 sleeves 730.00
Accessories
7500262 Vertical Bars, long, 64 cm, 2 290.00
7500263 Vertical Bars, short, 10 cm, 2 135.00
7500264 Horizontal Bar Kit, includes 2 tie bars, 4 bar clamps 330.00
7500260 Column Clamp Set, includes 1 column clamp assembly 405.00
7500265 Bar Clamps, 5 315.00
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 129
Medium-Pressure Chromatography Systems
bio-rad.com/c96
C-96 Autosampler
Ordering Information
Catalog # Description Price, U.S. $
7605010 -96 Autosampler with Cooling, 110–240 V, includes standard 84+3 vial tray (1.5 and 10 ml), control cable
C Inquire
set to connect with BioLogic DuoFlow system, 1 ml syringe, 2 ml sample loop; also includes #7605014,
#7605026, and #7600604
7605011 C-96 Autosampler, 110–240 V, includes standard 84+3 vial tray (1.5 and 10 ml), control cable set Inquire
to connect with BioLogic DuoFlow system, 1 ml syringe, 2 ml sample loop; also includes #7605014,
#7605026, and #7600604
7605012 Prep Bio Kit, contains 24-position tray for 10 ml vials (22 mm OD), 2.5 ml syringe, 10 ml PEEK loop, 6,895.00
0.75 mm ID PEEK injection valve, prep needle, 6 mm fitting wrench, for use with C-96 autosamplers
7605013 Syringe, pkg of 1, 1 ml syringe, for use with C-96 autosamplers 645.00
7605014 Connector Kit, contains nuts and ferrules to plumb the syringe valve, for use with C-96 autosamplers 105.00
7605024 Sample Tray, pkg of 1, large-capacity 96-position tray for 1.5 ml vials (12 mm OD), for use with 1,805.00
C-96 autosamplers
7600604 PEEK Tubing, pkg of 1, 1/16" OD x 0.020" ID x 30' high-pressure tubing, rated to 5,000 psi, orange 390.00
7605026 Fittings Kit, contains 10-32 short nuts and ferrules to plumb the injection valve, for use with C-96 autosamplers 155.00
7605027 Needle, pkg of 1, 45 µl needle, for use with C-96 autosamplers 665.00
7605028 Prep Kit Needle, pkg of 1, needle for large sample volume, for use with C-96 autosamplers 665.00
7605017 Analytical Bio Kit, includes 250 µl syringe, 500 µl buffer tubing, 100 and 200 µl PEEK sample loops, 1,805.00
standard sample needle, 6 mm fitting wrench, for use with C-96 autosamplers
7885011 NGC Autosampler, includes NGC SIM and connector cable Inquire
7885012 NGC Autosampler with Cooling, includes NGC SIM and connector cable Inquire
For NGC system–compatible products, see pages 117–118 for ordering information.
130 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Medium-Pressure Chromatography Systems
bio-rad.com/fractioncollectors Fraction Collectors
Fraction Collectors
Ordering Information
Catalog # Description Price, U.S. $
7318122 Model 2110 Fraction Collector, 100/120 V 1,630.00
7318120 Model 2110 Fraction Collector, 220/240 V 1,715.00
Accessories
7318130 Carousel, 80-tube capacity 87.00
7318135 Micro Tube Adaptor, 80 microtube capacity 44.00
7318136 Instrument Dust Cover 150.00
7318131 Replacement Drop Formers, 2 31.00
7318261* System Cable 1, 8-pin mini-DIN to DB-9 connector 92.00
7318265* System Cable 5, DB-9 connector to bare wires 92.00
7319010* System Cable 22, Y-cable connecting Econo gradient pump to Model 2110 fraction collector 235.00
Tubes
2239750 Clear Polystyrene Tubes, 13 x 100 mm, 9 ml nominal capacity, 1,000 51.00
2239751 Natural Polypropylene Tubes, 13 x 100 mm, 9 ml nominal capacity, 1,000 51.00
2239500 Micro Test Tubes, capless, 1.5 ml, polypropylene, natural, graduated, 500 24.00
* For more information, refer to the Cable Guide on page 134.
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 131
Medium-Pressure Chromatography Systems
Fraction Collectors bio-rad.com/fractioncollectors
132 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Medium-Pressure Chromatography Systems
bio-rad.com/fractioncollectors Fraction Collectors
Ordering Information
Catalog # Description Price, U.S. $
7410002 ioFrac Fraction Collector, input voltage 100/240 V, includes power cord, rack set F1
B 5,415.00
(2 x flatpack, 13 mm), BioFrac diverter valve, fittings kit
Accessories
7410010 ack Set F1, 2 x flatpack, with numbered tube positions, each holds 90 tubes, 12–13 mm diameter
R 145.00
7410011 Rack Set F2, 2 x flatpack, each holds 60 tubes, 15–16 mm diameter, for total of up to 120-tube collection 145.00
7410012 Rack Set F3, 2 x flatpack, each holds 40 tubes, 18–20 mm diameter, for total of up to 80-tube collection 145.00
7410013 Rack Set H1, 4 x flatpack, each holds 42 capless 1.5 ml Eppendorf/microtubes for total of up to 235.00
168-microtube collection
7410014 Rack Set H2, 4 x flatpack, each holds 63 capless 0.5 ml Eppendorf/microtubes for total of up to 235.00
252-microtube collection
7410015 Rack Set H3, 4 x flatpack, each holds 30 reduced-volume scintillation vials, 16 mm diameter, 235.00
for total of up to 120-vial collection
7410016 Rack Set H4, 4 x flatpack, each holds 6 scintillation vials, 30 mm diameter, for total of up to 24-vial collection 235.00
7410020 BioFrac H4-High Rack Set, 4 x flatpack, each holds 6 centrifuge tubes, 30 mm diameter, 175.00
for total of up to 24-vial collection
7410017 BioFrac Ice Bath/Microplate Rack, holds 120 tubes, 12–13 mm diameter; with the following capabilities: 265.00
up to 4 SBS-format microplates in 96-, 48-, 24-, or 12-well configurations; Titertube microtube collection,
8 x 12, 96-tube configuration
7410018 BioFrac Prep-20 Preparative Rack, for fractionation into 1–20 collection vessels of any size 145.00
7410007 BioFrac Fraction Collector Fittings Kit, includes replacement fittings and tubing for setup of the fraction 175.00
collector to the BioLogic LP or BioLogic DuoFlow chromotography system
7410088 BioFrac Microplate Drophead Kit, includes preassembled drophead nut with 0.020" ID Tefzel tubing; 100.00
delivers approximately 25 µl per drop
7318263* System Cable 3, 8-pin mini-DIN to 8-pin mini-DIN 92.00
7318286* System Cable 15, 15-pin D to mini-DIN 155.00
7318287* System Cable 16, 8-pin mini-DIN to 8-pin standard DIN 135.00
7318290 BioFrac Accessory Cable, 15-pin D to bare wires, for connecting BioFrac fraction collector to other equipment; 185.00
for input or output signals
Tubes
2239750 Clear Polystyrene Tubes, 13 x 100 mm, 9 ml nominal capacity, 1,000 51.00
2239751 Natural Polypropylene Tubes, 13 x 100 mm, 9 ml nominal capacity, 1,000 51.00
2239500 Micro Test Tubes, capless, 1.5 ml, polypropylene, natural, graduated, 500 24.00
* For more information, refer to the Cable Guide on page 134.
For NGC system–compatible products, see pages 117–118 for ordering information.
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 133
Chromatography Accessories
bio-rad.com/chromaccessories
Chromatography Accessories
Cables
Refer to the guide to select cables to link chromatography system components together or to link components from other
manufacturers to a Bio-Rad chromatography system. Numbers in the table are cable numbers.
For More Information
Web: bio-rad.com/chromaccessories
Cable Guide
Connection to
BioLogic Model Model Model
BioLogic BioLogic™ BioLogic QuadTec™ Non-BioLogic Non-BioLogic 2110 BioFrac™ EM-1 EP-1 Econo
DuoFlow™ LP DuoFlow UV/Vis System System Fraction Fraction Econo™ UV Econo Gradient
Connection From Workstation System Controller Detector Compromise Detectors Collector Collector Monitor Pump Pump
BioLogic DuoFlow 17, 18, 17, 18, 5 17, 18, 7 17, 18,
workstation 19, or 21 25, or 26 19, or 21 19, or 21
Model 2110 5 1 5 1 1 22
fraction collector
BioFrac fraction 17, 18, 3, 15 * * 3, 15 23
collector 19, or 21
Isco Retriever II 12 12
collector
Gilson FC 203 14 14
fraction collector
GE Healthcare FRAC-100 9 9
fraction collector
Model EG-1 Econo
gradient monitor 7
Model EM-1 Econo* 4 3
UV monitor
Model EP-1 Econo pump 7 7 1 3, 15 3
Econo gradient pump 17, 18, 22 23
19, or 21
* For replacement of the Model 2128 accessory cable, order the BioFrac accessory cable.
Ordering Information
Catalog # Description Price, U.S. $
7318261 System Cable 1, 8-pin mini-DIN to DB-9 connector 92.00
7318262 System Cable 2, 8-pin mini-DIN to 8-pin standard DIN 92.00
7318263 System Cable 3, 8-pin mini-DIN to 8-pin mini-DIN 92.00
7318264 System Cable 4, 8-pin mini-DIN to banana plug cable 92.00
7318265 System Cable 5, DB-9 connector to bare wires 92.00
7318267 System Cable 7, 8-pin mini-DIN to bare wires 140.00
7318268 System Cable 8, 8-pin standard DIN to DB-9 connector 92.00
7318286 System Cable 15, 15-pin D to mini-DIN 155.00
7318287 System Cable 16, 8-pin mini-DIN to 8-pin standard DIN 135.00
7500650 System Cable 17, bus communication cable, 1.2 m (4') 64.00
7500651 System Cable 18, bus communication cable, 3.7 m (12') 77.00
continues
134 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Chromatography Accessories
bio-rad.com/chromaccessories
Ordering Information
Catalog # Description Price, U.S. $
7500652 System Cable 19, bus communication cable, 9.2 m (30') 140.00
7500655 System Cable 21, BioLogic HR system communication cable, 30 m (100') 300.00
7319010 System Cable 22, Y-cable for connecting Econo gradient pump to Model 2110 fraction collector 235.00
7601307 System Cable 25 (BioLogic QuadTec RS-232), connects BioLogic QuadTec to ICM 57.00
7601321 System Cable 26 (ICM Power), connects to 12 V power on BioLogic DuoFlow workstation 67.00
7602004 System Cable 30, bus communication cable, 0.3 m (1') 33.00
7602032 System Cable 31, USB cable 35.00
7318290 BioFrac Accessory Cable, 15-pin D to bare wires, for connecting BioFrac fraction collector 185.00
7885013 NGC Autosampler Cables, for connection with the NGC SIM 125.00
For NGC systems ordering information see page 114.
For BioLogic DuoFlow systems ordering information see page 123.
Low-Pressure Tubing
■■ Siliconetubing — contains no cytotoxic extractables ■■ PTFE tubing — chemically inert; can be used with
and has excellent wetting properties. Autoclavable and virtually any reagent, stable up to 400°C
heatable for pyrogen removal. May be damaged by
concentrated acids and bases Pump Tubing Kits
Precut tubing for the Model EP-1 Econo pump, Econo
■■ Tygon tubing — clear and tough, tolerates greater
gradient pump, and BioLogic LP system is available in
pressures than silicone; transparent; should not be
PharMed and silicone with a choice of 0.8, 1.6, or 3.2 mm
autoclaved; may be damaged by high concentrations
ID. Each kit contains 20 pieces of precut pump tubing and
of alcohol
four sets of luer lock fittings and tubing retainers.
■■ PharMed tubing — has a wider range of chemical
compatibility than silicone or Tygon and is ideal for use
in pump heads of the Model EP-1 Econo™ pump, Econo
gradient pump, and BioLogic™ LP system; lasts ten times
longer than Tygon or silicone
0.51 x 0.8 mm
Tubing size (ID x wall)
0.8 x 0.8 mm
1.6 x 0.8 mm
3.2 x 0.8 mm
0.001
0.002 0.005 0.01 0.02 0.05 0.10 0.20 0.5 1.0 2 5 10 20
Flow rate, ml/min
Tubing size selection. Comparison of flow rate ranges of various tubing sizes (ID) when used in the Econo gradient pump,
the Model EP-1 Econo pump, and the BioLogic LP system.
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Chromatography Accessories
bio-rad.com/chromaccessories
Ordering Information
Catalog # Description Price, U.S. $
Silicone Tubing
7318210 Silicone Tubing, 0.8 mm ID/0.8 mm wall, 10 m 81.00
7318211 Silicone Tubing, 1.6 mm ID/0.8 mm wall, 10 m 89.00
7318212 Silicone Tubing, 3.2 mm ID/0.8 mm wall, 10 m 100.00
Tygon Tubing
7318213 Tygon Tubing, 0.51 mm ID/0.8 mm wall, 10 m 48.00
7318214 Tygon Tubing, 0.8 mm ID/0.8 mm wall, 10 m 54.00
7318215 Tygon Tubing, 1.6 mm ID/0.8 mm wall, 10 m 54.00
PharMed Tubing
7318207 PharMed Tubing, 0.8 mm ID/1.0 mm wall, 10 m 95.00
7318208 PharMed Tubing, 1.6 mm ID/1.0 mm wall, 10 m 92.00
7318209 PharMed Tubing, 3.2 mm ID/1.0 mm wall, 10 m 100.00
Pump Tubing Kits
7318240 Pump Tubing Kit, 0.8 mm ID silicone, 20 precut lengths and 4 sets of fittings, for use with EP-1 Econo pump 93.00
7318241 Pump Tubing Kit, 1.6 mm ID silicone, 20 precut lengths and 4 sets of fittings, for use with EP-1 Econo pump 93.00
7318242 Pump Tubing Kit, 3.2 mm ID silicone, 20 precut lengths and 4 sets of fittings, for use with EP-1 Econo pump 99.00
7318247 Pump Tubing Kit, 0.8 mm ID PharMed, 20 precut lengths and 4 sets of fittings 110.00
7318248 Pump Tubing Kit, 1.6 mm ID PharMed, 20 precut lengths and 4 sets of fittings 99.00
7318249 Pump Tubing Kit, 3.2 mm ID PharMed, 20 precut lengths and 4 sets of fittings 115.00
7319007 Econo Gradient Pump Tubing Kit, includes 2 each of 0.8, 1.6, and 3.2 mm PharMed tubing, for use with 92.00
Econo gradient pump
■■ PTFE FEP tubing — recommended for pre-pump chemically inert and autoclavable. Tefzel is translucent
connections; can withstand medium pressure; translucent, and slightly flexible. PEEK is opaque; the color indicates
semiflexible, chemically inert, and autoclavable the inside diameter
■■
or PEEK tubing — recommended for post-pump
Tefzel ■■ Tubing kits — set of premade tubing with fittings to
connections; both withstand high pressure and are simplify setup of the BioLogic DuoFLow™ system
Ordering Information
Catalog # Description Price, U.S. $
7500603 TFE FEP Tubing, 1/8" (0.125", 3.2 mm) OD x 0.062" (1.6 mm) ID, 15' (4.6 m),
P 69.00
for pre-pump buffer inlet lines to the pump heads
7500602 Tefzel Tubing, 1/16" (0.062", 1.6 mm) OD x 0.020" (0.5 mm) ID, 30' (9.1 m), 150.00
for system connections post-pump
7600604 PEEK Tubing, orange, 1/16" OD x 0.020" ID x 30', rated to 5,000 psi 390.00
7600605 PEEK Tubing, green, 1/16" OD x 0.030" ID x 30', rated to 3,000 psi 140.00
7600650 F10 Tubing Kit, includes precut and fitted PTFE, Tefzel, and orange PEEK tubing for installation of 405.00
BioLogic DuoFlow basic chromatography system running at flow rates <40 ml/min
7600652 F40 Tubing Kit, includes precut and fitted PTFE, Tefzel, and green PEEK tubing for installation of 390.00
BioLogic DuoFlow basic chromatography system running at flow rates ≥40 ml/min
7602046 pH Tubing Kit, includes orange and green PEEK 1/4–28 prefitted tubing lengths for connection of the 79.00
pH flow cell to the chromatography system
7602002 BioLogic Maximizer Tubing Kit, includes 4 PTFE FEP prefitted tubing lengths for connection of solvent 180.00
vials to the BioLogic Maximizer mixer; color coding indicates buffer solution
7602003 BioLogic Maximizer Interconnect Tubing, includes 2 PEEK prefitted tubing lengths for connection of 105.00
BioLogic DuoFlow pumps to the BioLogic Maximizer valve system
136 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Chromatography Accessories
bio-rad.com/chromaccessories
The BioLogic DuoFlow™ fittings kit includes all parts necessary to connect medium- and high-pressure columns to
medium-pressure chromatography systems.
For More Information
Request or download bulletin: column connection instructions — 5326
Ordering Information
Catalog # Diagram Description Quantity Price, U.S. $
Medium- and High-Pressure Fittings Kit
7600550 BioLogic System Fittings Kit, includes PEEK and Tefzel nuts, ferrules, unions, 1 535.00
plugs, and luer syringe
Individual Medium- and High-Pressure Fittings
7885015 PEEK Nut, 1/8", 10 10 4.00
7885010 Adaptor, FEM Slip Luer-FEM 10-32 + Adap, Quick Con, M Luer-FEM 10-32 1 175.00
Luer to BioLogic System Fittings Kit, includes 1/4–28 female to male luer, 1/4–28
7320113 1 59.00
female to female luer, to connect 1 cartridge to a BioLogic DuoFlow system
7500556 Ferrule and Lock Ring, for 1/16" OD (1.6 mm) tubing 10 67.00
7500559 Tefzel Cap, 1/4–28 female connection, to plug unused tubing 5 35.00
7500563 Tefzel Plug, 1/4–28 male connection, to plug unused ports on valves and columns 5 23.00
PLC Column to BioLogic System Adaptors, 2 fittings to connect
H
7500564 2 60.00
1 HPLC column (10-32) to a BioLogic DuoFlow system
Econo-Column to BioLogic System Fittings Kit, 2 fittings to connect
7500565 1 set 60.00
1 Econo-Column (luer) column to a BioLogic DuoFlow system
7500566 Bottle Cap Kit, includes 2 bottle caps, 2 plugs 1 set 78.00
NO M6 Fittings Kit, includes 2 nuts and 4 ferrules to connect
U
7500567 1 set 36.00
UNO column to an FPLC system
UNO 10-32 Fittings Kit, includes 2 nuts and 4 ferrules to connect
7500568 1 set 47.00
UNO column to an HPLC system
7500569 Delrin Nut, for 1/16" OD (1.6 mm) tubing 10 16.00
7500571 Ferrule and Lock Ring, for 1/8" OD (3.2 mm) tubing 5 30.00
7500703 Inline Filter Kit, includes 1 inline filter and 2 replacement frits 1 94.00
7500553 1/8" OD (3.2 mm) Pre-Pump Fittings, includes Delrin nut, ferrules, lock ring 5 78.00
7500554 1/16" OD (1.6 mm) Post-Pump Fittings, includes Delrin nut, ferrules, lock ring 10 185.00
7601308 Long Fingertight Fittings, 10-32 x 0.82", for PEEK and Tefzel tubing 4 41.00
7601311 Long Fingertight Fittings, 10-32 x 1.03", for PEEK and Tefzel tubing 4 51.00
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 137
Chromatography Accessories
bio-rad.com/chromaccessories
Low-Pressure Fittings
The low-pressure system fittings kit is useful to adapt various tubing sizes, to make liquid connections,
and to direct and stop liquid flow.
For More Information
Request or download bulletin: column connection instructions — 5326
Ordering Information
Catalog # Description Price, U.S. $
Medium- and High-Pressure Fittings Kit
7318220 Low-Pressure System Fittings Kit, polycarbonate/polypropylene, 250 pieces 340.00
7319006 Econo Gradient Pump Fittings Kit, includes 32 fittings, 12 tubing retainers 100.00
138 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Low-Pressure Chromatography Systems
bio-rad.com/lowpressure
BioLogic™ LP Systems
7 5
7
8
3
4
1
The BioLogic LP low-pressure chromatography system ■■ LP Data View™ software — easy-to-use software
offers high performance, versatility, ease of use, and designed for the BioLogic LP system. The software
affordability. Its compact design minimizes the workspace captures data, multitasks, and prints data from any
required in the coldroom or on the laboratory bench. The computer that runs Windows XP or Windows 7 operating
BioLogic LP system includes features such as: systems; requires use of one serial port
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 139
Low-Pressure Chromatography Systems
bio-rad.com/lowpressure
Ordering Information
Catalog # Description Price, U.S. $
7318300 tandard BioLogic LP System, 100/120 V, includes BioLogic LP controller, BioLogic rack, accessory kit with
S Inquire
MV-6 manual inject valve, proportioning valve/mixer, UV optics, conductivity cell, tubing and fittings, column and
conductivity cell holder, starter kit
7318301 Standard BioLogic LP System, 220/240 V, includes same as #7318300 9,345.00
7318302 BioLogic LP System with Model 2110 Fraction Collector, 110/120 V, includes standard BioLogic LP system, Inquire
SV-3 diverter/bypass valve, system cable 1
7318303 BioLogic LP System with Model 2110 Fraction Collector, 220/240 V Inquire
7318304 BioLogic LP System with BioFrac Fraction Collector, 110/120 V, includes standard BioLogic LP system, Inquire
system cables 3 and 15
7318305 BioLogic LP System with BioFrac Fraction Collector, 220/240 V Inquire
7318336 BioLogic LP System with Model 2110 Fraction Collector and LP Data View Software, 110/120 V, Inquire
includes SV-3 diverter/bypass valve, system cable 1, 25' serial cable
7318337 BioLogic LP System with Model 2110 Fraction Collector and LP Data View Software, 220/240 V Inquire
7318338 BioLogic LP System with BioFrac Fraction Collector and LP Data View Software, 110/120 V, Inquire
includes system cables 3 and 15, 25 serial cable
7318339 BioLogic LP System with BioFrac Fraction Collector and LP Data View Software, 220/240 V Inquire
Pump Tubing Kits
7318320* MV-6 Manual Inject Valve, 6 ports 480.00
7318321* SV-5 Buffer Select Valve, 5-port, 4-position solenoid random-access valve, 30 psi (2 bar) limit 1,160.00
7318322* SV-3 Diverter/Bypass Valve, 3-way valve 545.00
7318323 Gradient Mixer 935.00
7318324 BioLogic LP Optics Module 2,670.00
7318165 UV Flow Cell, replacement 345.00
7318166 Lamp, replacement 350.00
7318167 Filter Assembly, 254 and 280 nm 460.00
7318155 Conductivity Flow Cell 610.00
7318350 BioLogic LP Starter Kit, includes buffers, standard, anion exchange cartridge 125.00
* For more information, see page 141.
140 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Low-Pressure Chromatography Systems
bio-rad.com/LPcomponents
Ordering Information
Catalog # Description Price, U.S. $
7318365 LP Data View Software for the BioLogic LP System, includes software CD, cable adaptor 485.00
BioLogic™ LP Valves
Ordering Information
Catalog # Description Price, U.S. $
7318321 SV-5 Buffer Select Valve, 5-port, 4-position solenoid random-access valve, 30 psi (2 bar) limit 1,160.00
7318320 MV-6 Manual Inject Valve, 6 ports 480.00
7318322 SV-3 Diverter/Bypass Valve, 3-way valve 545.00
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 141
Low-Pressure Chromatography Systems
bio-rad.com/lowpressure
■■
Simple setup and programming
■■
Flow rates of 0.1–40 ml/min
■■
Control of a gradient mixer for binary gradient formation
6
■■
Control of an optional fraction collector and diverter valve
■■
utomated calibration procedures for a variety of
A
tubing sizes
1
■■
Maximum pressure of 30 psi
■■
ompatible with the BioLogic DuoFlow™
C
chromatography systems
For More Information
Web: bio-rad.com/econogradient
Request or download bulletin: 2438
Ordering Information
Catalog # Description Price, U.S. $
7319001 Econo Gradient Pump, 100/120 V, includes tubing and fittings kits 3,825.00
7319002 Econo Gradient Pump, 220/240 V 3,825.00
Combination Systems
7319030 Econo Gradient Pump Combo 1, 100/120 V, includes Econo gradient pump, gradient mixer valve 4,580.00
7319032 Econo Gradient Pump Combo 1, 220/240 V 4,360.00
7319034 Econo Gradient Pump Combo 2, 100/120 V, includes Econo gradient pump, gradient mixer valve, 5,440.00
MV-6 manual inject valve, rack with column clamps
7319036 Econo Gradient Pump Combo 2, 220/240 V 5,255.00
7319038 Econo Gradient Pump Combo 3, 100/120 V, includes Econo gradient pump, gradient mixer valve, 5,015.00
rack with column clamps
7319040 Econo Gradient Pump Combo 3, 220/240 V 4,845.00
Valves
7318322 SV-3 Diverter/Bypass Valve, 3-way valve 545.00
7318323 Gradient Mixer 935.00
7318320 MV-6 Manual Inject Valve, 6 ports 480.00
Cables*
7319010 System Cable 22, Y-cable for connecting Econo gradient pump to Model 2110 fraction collector 235.00
Accessories
7319004 Econo Gradient Pump Rack, preassembled 645.00
7319006 Econo Gradient Pump Fittings Kit, includes 32 fittings, 12 tubing retainers 100.00
7319007 Econo Gradient Pump Tubing Kit, includes 2 each of 0.8, 1.6, and 3.2 mm PharMed tubing 92.00
* For more information, refer to the Cable Guide on page 134.
142 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Low-Pressure Chromatography Systems
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Specifications
Wavelength 254 and 280 nm Noise 1.0 x 10 –4 OD max. peak-to-peak
Sensitivity ranges 2.0, 1.0, 0.5, 0.2, 0.1, 0.05, 0.02, 0.01 AUFS (dry cell); 2.0 x 10 –4 OD max.
Detection limit 7 µg/ml (BSA in H2O) peak-to-peak (flowing liquid)
Lamp Low-pressure mercury with phosphor screen Safety Meets IEC 61010 and CSA
Filters 254 and 280 nm 22.2 certification
Output signal 0–1 V analog (impedance 150 W) Dimensions (W x D x H) Base unit: 14.6 x 18.6 x 20.2 cm
Operating temperature 4–40°C Optics unit: 13.2 x 15.2 x 3.8 cm
Flow cell Optical path 2 mm, internal volume 80 µl,
illuminated volume 3 µl
Ordering Information
Catalog # Description Price, U.S. $
7318160 odel EM-1 Econo UV Monitor, 100/120 V, includes control module, optics module, filters for 254 and
M 3,690.00
280 nm wavelengths, system cable 4, fittings kit
7318162 Model EM-1 Econo UV Monitor, 220/240 V 3,815.00
Accessories
7318165 UV Flow Cell, replacement 345.00
7318166 Lamp, replacement 350.00
7318167 Filter Assembly, 254 and 280 nm 460.00
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 143
Process-Scale Separations
bio-rad.com/process
Process-Scale Separations
Bio-Rad has extensive experience serving the separation technology community with process resins that offer
optimal solutions for your separation needs. To order Bio-Rad process-scale resins, contact your local Bio-Rad
sales representative.
144 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Process-Scale Separations
bio-rad.com/process
Nuvia™ resins — a family of next-generation ion-exchange MPC™ media — MPC is a fluorinated derivative of
products built on an industry proven rigid polymer base ceramic hydroxyapatite providing the same robust impurity
matrix. Nuvia Q and S resins offer superior flow properties clearance as CHT Type I, 40 μm. Use MPC to maximize
and low nonspecific binding while delivering high capacity your process economics for specialty protein purification
and unique selectivity. Nuvia HR-S is a high-resolution cation applications. Contact your local Bio-Rad representative for
exchanger capable of separating very challenging high additional information.
molecular weight impurities. Nuvia resins bring together a For More Information
unique set of properties specifically designed to meet the Web: bio-rad.com/MPC
demands of current and future downstream processes.
Nuvia resins are flexible and robust with a large operational Nuvia™ cPrime™ resin — this resin is designed for
window, making them effective in capture and/or polish steps. process-scale purification of a wide variety of therapeutic
proteins. The unique selectivity allows method developers to
For More Information
Web: bio-rad.com/nuvia
use hydrophobic and cation exchange interaction modes to
achieve effective purification. The resins have a large design
UNOsphere™ resins — specialized for fast mass transfer, space for binding and elution, allowing for the development
UNOsphere resins deliver high binding capacity at high flow of highly robust methods in a commercial manufacturing
rates while maintaining low backpressure. These robust setting. Nuvia cPrime is built on a rigid, mechanically and
polymeric resins formed by single-step polymerization carry chemically stable macroporous base matrix with a particle
either ionic (UNOsphere Q and S) or affinity (UNOsphere size optimized to deliver exceptional flow properties, fast
SUPrA™) functionality, making them ideal for the efficient mass transfer, and stability.
purification of biopharmaceutical molecules from feed
For More Information
stream at any stage of the downstream process. Web: bio-rad.com/nuvia
For More Information
Web: bio-rad.com/processIEX CFT™ ceramic fluoroapatite media — this chemically pure
form of fluoroapatite is a rigid, spherical, and macroporous
Macro-Prep® resins — polymeric methacrylate resins are media used in the purification of biologically significant
available with strong or weak ion exchange functionalities. compounds. It is ideally suited for the bioprocessing industry.
These rigid macroporous hydrophilic resins provide excellent CFT can be used under stringent conditions to separate
dynamic binding capacity, resolution, and throughput at high acidic proteins requiring buffered conditions as low as
flow rates for the purification of biomolecules. Macro-Prep pH 5.6. CFT has high binding capacity and may be used
resins are an excellent choice for process-scale applications reproducibly over an extended number of chromatography
such as blood fractionation purification. runs. When CFT is used, process engineers can perform
For More Information purifications across a range of lower pH values to obtain
Web: bio-rad.com/processIEX optimal and reproducible results for the targeted biomolecule.
CHT ™ ceramic hydroxyapatite media — CHT is a robust For More Information
mixed-mode media with unique separation properties Web: bio-rad.com/processCFT
that delivers exceptional selectivity for purification of AG® resin — AG resins are a highly referenced and
biomolecules. As a proven scalable polishing step in extensively used line of chromatography resins for the
mAb and vaccine purification, CHT effectively eliminates separation of low molecular weight molecules, such as
common feedstream contaminants, such as aggregates, inorganic ions, organic acids, peptides, and carbohydrates,
leached Protein A, DNA, and host cell proteins, in a single from biopharmaceutical preparations and ancillary buffers
step. This provides you the flexibility to maximize your used in biomanufacturing. They are highly processed to
process economics. Use CFT ™ ceramic fluoroapatite for remove impurities. Biotechnology-grade AG resins are
protein separations requiring acidic-buffered conditions. further treated to reduce bioburden to extremely low levels,
For More Information making them suitable for process-scale purification of
Web: bio-rad.com/processCHT biopharmaceuticals.
For More Information
Web: bio-rad.com/processAG
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 145
Process-Scale Separations
bio-rad.com/process
Ordering Information
Catalog # Description Price, U.S. $
Foresight Plates, 2 x 96-Well
7324714 Foresight UNOsphere Q, 20 µl 500.00
7324710 Foresight UNOsphere S, 20 µl 500.00
7324709 Foresight UNOsphere SUPrA, 20 µl Inquire
7324703 Foresight Nuvia Q, 20 µl 500.00
7324701 Foresight Nuvia S, 20 µl 500.00
7324707 Foresight Nuvia HR-S, 20 µl 500.00
7324705 Foresight Nuvia cPrime, 20 µl 500.00
7324716 Foresight CHT Type I, 40 µm, 20 µl 500.00
7324718 Foresight CHT Type II, 40 µm, 20 µl 500.00
7324785 Foresight MPC Type I, 40 µm, 20 µl 500.00
continues
146 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Process-Scale Separations
bio-rad.com/process
Ordering Information
Description 1 ml Price, U.S. $ 5 ml Price, U.S. $
Foresight Columns
Foresight UNOsphere Q 7324732 95.00 7324752 173.00
Foresight UNOsphere S 7324730 95.00 7324750 173.00
Foresight UNOsphere SUPrA 7324729 190.00 7324749 565.00
Foresight Nuvia Q 7324721 102.00 7324741 188.00
Foresight Nuvia S 7324720 102.00 7324740 188.00
Foresight Nuvia HR-S 7324723 115.00 7324743 210.00
Foresight Nuvia cPrime 7324722 112.00 7324742 212.00
Foresight CHT Type I, 40 µm 7324735 125.00 7324755 245.00
Foresight CHT Type II, 40 µm 7324736 125.00 7324756 245.00
Foresight MPC Type I, 40 µm 7324737 129.00 7324757 252.00
Foresight RoboColumn Units, 1 row of 8 columns*
Foresight UNOsphere Q RoboColumn Unit 7324819 175.00 7324820 210.00
Foresight UNOsphere S RoboColumn Unit 7324813 175.00 7324814 210.00
Foresight UNOsphere SUPrA RoboColumn Unit 7324834 475.00 7324835 600.00
Foresight Nuvia Q RoboColumn Unit 7324804 180.00 7324805 225.00
Foresight Nuvia S RoboColumn Unit 7324801 180.00 7324802 225.00
Foresight Nuvia HR-S RoboColumn Unit 7324831 185.00 7324832 235.00
Foresight Nuvia cPrime RoboColumn Unit 7324807 182.00 7324808 233.00
Foresight CHT Type I, 40 µm RoboColumn Unit 7324822 185.00 7324823 240.00
Foresight CHT Type II, 40 µm RoboColumn Unit 7324825 185.00 7324826 240.00
Foresight MPC Type I, 40 µm RoboColumn Unit 7324828 210.00 7324829 240.00
* Foresight RoboColumn units are to be used with robotic liquid handling workstations.
Please visit bio-rad.com/foresight for more information on prepacked columns and plates.
In the U.S., call toll free at 1-800-424-6723 Chromatography: Laboratory and Process Separations | 147
Chromatography: Laboratory and Process Separations
Subject Index
Subject Index
Affi-Gel® 10 and Affi-Gel 15 Resins, 83 DEAE Affi-Gel® Blue Resin, 80
®
Affi-Gel and Affi-Prep® Protein A Resins, 79 Econo-Column® Flow Adaptors, 108
Affi-Gel® Blue Resin, 80 Econo-Column® Funnel, 109
Affi-Gel® Boronate Resin, 81 Econo™ Gradient Pump, 142
Affi-Gel® Heparin Resin, 82 Econo-Pac® Affinity and Desalting Columns, 102
Affi-Gel® Hz Hydrazide Resin, 84 Econo-Pac® Columns, 104
Affi-Prep® Polymyxin Resin, 82 ENrich™ High-Resolution Ion Exchange Columns, 92
AG® Bio-Rex™, and Chelex® Resins, 61 ENrich™ High-Resolution Size Exclusion Columns, 93
®
Aminex HPLC Columns, 101 EZLogic™ Integration Software, 128
Bio-Beads™ SM-2 Adsorbents, 87 Foresight™ Prepacked Plates and Columns, 94–95, 146
Bio-Beads™ S-X Resins, 86 Glass Econo-Column® Columns, 105
BioFrac™ Fraction Collector, 132 Glass Econo-Column® Reservoirs, 109
Bio-Gel® A 1.5 m Resin, 86 High-Pressure Tubing and Tubing Kits, 136
Bio-Gel® Hydroxyapatite HT and HTP, 69 Jacketed Econo-Column® Columns, 107
Bio-Gel® P Resin, 85 Low-Pressure Fittings, 138
BioLogic DuoFlow™ Detectors, 125 Low-Pressure Tubing, 135
BioLogic DuoFlow™ IQ/OQ Protocols, 129 Macro-Prep® HIC Resins, 87
BioLogic DuoFlow™ Medium-Pressure Chromatography Macro-Prep® Ion Exchange Resin/Media, 59
Systems, 122 Medium- and High-Pressure Fittings, 137
BioLogic DuoFlow™ Software, Version 5.3, 128 Mini Bio-Spin™ Columns, 98
BioLogic DuoFlow™ Valves and Accessories, 126 Model 2110 Fraction Collector, 131
BioLogic DuoFlow™ Workstations and Accessories, 124 Model EM-1 Econo™ UV Monitor, 143
BioLogic™ LP Data View™ Software, 141 Molecular Biology and Biotechnology Grade Resins, 64
BioLogic™ LP Systems, 139 MPC™ Ceramic Hydroxyfluoroapatite, 67
BioLogic™ LP Valves, 141 NGC™ Medium-Pressure Chromatography Systems, 111
BioLogic™ Rack, 129 Nuvia™ cPrime™ Mixed-Mode Resin, 65
Bio-Scale™ CHT™ Type I Columns, 99 Nuvia™ IMAC Resin, 71
Bio-Scale™ Mini Cartridge Protein Purification Sampler Packs and Kits, 96 Poly-Prep® Columns, 104
Bio-Scale™ Mini Cartridges, 95 Poly-Prep® Ion Exchange Columns, 98
Bio-Scale™ Mini Kits, 89 Process-Scale Chromatography Resin, 145
Bio-Scale™ Mini Nuvia™ IMAC Columns, 70 Profinia™ System Reagents and Kits, 75
Bio-Scale™ MT High-Resolution Columns, 110 Profinity™ Epoxide Resin, 83
Bio-Spin® and Micro Bio-Spin™ Columns, 97 Profinity eXact™ Resins and Cartridges, 73
Bio-Spin®, Micro Bio-Spin™, and Mini Bio-Spin Columns, 103 Profinity™ GST Cartridges and Kits, 73
C-96 Autosampler, 130 Profinity™ IMAC Resins and Cartridges, 72
Cables, 134 Sampler Packs, 88
Carbodiimide Activated Resin, 84 Technical and Reactor Grade Resins, 63
CFT™ Ceramic Fluoroapatite, 68 UNO® Monolith Ion Exchange Columns, 100
Chromatography Standards, 89 UNOsphere™ and Nuvia™ Ion Exchange Resins/Media, 57
ChromLab™ Software, 119 UNOsphere SUPrA™ rProtein A Resin/Media, 78
ChromLab™ Software, IQ/OQ and 21 CFR Part 11 Compliant, 121
ChromLab™ Software, Security Edition, 121
ChromLab™ Software, User Management Edition, 121
CHT™ Ceramic Hydroxyapatite, 66
CM Affi-Gel® Blue Resin, 81
148 | Chromatography: Laboratory and Process Separations In the U.S., call toll free at 1-800-424-6723
Electrophoresis
and Blotting
Electrophoresis and Blotting Solutions 150
Protein Electrophoresis
A complete suite of electrophoresis products, from cells and precast gels to buffers and reagents, is available for the
separation of proteins.
Learn More About the Technology
Web: bio-rad.com/tech/proteinelectro
Buffers &
Reagents
page 192
Standards
page 157
Sample Electrophoresis
Preparation Systems & Gels Blotting Detection Imaging
Preparative Power
Electrophoresis Supplies
page 213 page 153
Wet/Tank Semi-Dry
Systems Systems
page 226 page 224
Conjugates
Gel Drying Stains Membranes
Chemiluminescence Colorimetric
and
Equipment pages 199, 243 Detection Detection
Reagents page 232
page 218 page 238 page 239
page 242
150 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Protein Electrophoresis
bio-rad.com/proteinelectroblot
Advantages
Run 1–4 precast or handcast Fast setup with drop-in gel and Large-format gel system offers Offers maximum resolution in a
gels in mini format with the cell design (precast or handcast) greater resolution over smaller single gel and the longest range of
Mini-PROTEAN Tetra cell Run 1–2 precast Criterion or formats and can be used with MW separation
Wing clamp assembly allows handcast gels with the Criterion cell handcast gels Run up to 12 gels with the
easy, fast setup and leak-free Integrated upper buffer chamber Versatility to perform 1-D or PROTEAN Plus Dodeca cell
operation ensures leak-free operation 2-D gel electrophoresis
Minimize reagent cost and Optimal combination of pl Can accommodate up to 4 gels
waste separation and fast run times and is available in xi or XL format
Fastest turnaround of 2-D data for running a variety of gel sizes
Capability for <1 hr 2-D runs
for 2-D-in-a-day capability for 2-D-in-a-day results Multi-cell is available for
Run up to 12 mini handcast running up to 6 gels
Run up to 12 midi handcast
or precast gels with the or precast gels with the
Mini-PROTEAN® Dodeca™ cell Criterion Dodeca cell
Electrophoresis Cells
Mini-PROTEAN Tetra Criterion (page 175) PROTEAN II xi/XL (page 184) PROTEAN Plus Dodeca
(page 164)
Criterion
Dodeca (page 176) PROTEAN II xi/XL multi-cells (page 189)
Mini-PROTEAN 3 Dodeca (pages 186)
(page 167)
continues
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 151
Protein Electrophoresis
bio-rad.com/proteinelectroblot
Precast Gels
Bio-Rad offers a broad range of precast gels, including two size formats of polyacrylamide gels, for a number of vertical protein and
nucleic acid electrophoresis applications, and one set of agarose gels for horizontal nucleic acid electrophoresis. These gels are part
of complete systems of compatible electrophoresis and blotting cells. Refer to the following table to select the appropriate gel type and
buffers for your polyacrylamide gel–based applications.
In general, single-percentage gels will best separate bands that are close in MW. If your sample contains a broad range of MWs,
a gradient gel allows both high- and low-MW bands to be resolved on the same gel. Molecules with a range of sizes can be separated
on linear gradient gels because the larger pore sizes allow resolution of larger molecules, while pore sizes that decrease toward the
bottom of the gel restrict excessive separations of small molecules.
152 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Protein Electrophoresis
bio-rad.com/powersupplies Power Supplies
Power Supplies
Bio-Rad offers a complete line of power supplies that are certified to IEC 1010-1, EN 61010 — the most rigorous
international safety standard — to ensure the highest personal and environmental protection.
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 153
Protein Electrophoresis
Power Supplies bio-rad.com/powersupplies
154 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Protein Electrophoresis
bio-rad.com/powersupplies Power Supplies
Ordering Information
Catalog # Description Price, U.S. $
1645050 PowerPac Basic Power Supply, 100–120/220–240 V 360.00
Ordering Information
Catalog # Description Price, U.S. $
1645052 PowerPac HC Power Supply, 100–120/220–240 V 820.00
Ordering Information
Catalog # Description Price, U.S. $
1645056 PowerPac HV Power Supply, 100–120/220–240 V 2,325.00
1645059 PowerPac HV Power Supply with Temperature Probe, 100–120/220–240 V 2,725.00
1645097 PowerPac Data Transfer Software, version 2.0 255.00
1645099 PowerPac HV IQ/OQ Protocol Binder 1,110.00
1655058 PowerPac Temperature Probe 280.00
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 155
Protein Electrophoresis
Power Supplies bio-rad.com/powersupplies
Ordering Information
Catalog # Description Price, U.S. $
1645070 PowerPac Universal Power Supply, 100–120/220–240 V 2,385.00
1645097 PowerPac Data Transfer Software, version 2.0 255.00
1645069 PowerPac Universal IQ/OQ Protocol Binder and Test Box 2,210.00
PowerPac™ Adaptor
■■
onvert non–IEC-certified electrophoresis cells to fit output terminals of PowerPac
C
power supplies
■■
Available in two sizes that fit most 2 and 4 mm banana plugs
■■
Compatible with the discontinued PowerPac 200, 300, 1000, and 3000 power supplies
Ordering Information
Catalog # Description Price, U.S. $
1645062 PowerPac Adaptor, 2 mm 59.00
1645064 PowerPac Adaptor, 4 mm 59.00
156 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Protein Electrophoresis
bio-rad.com/proteinstandards Protein Standards
Protein Standards
Standards are an integral part of every electrophoresis experiment because they help identify and characterize the molecules separated
in a gel. Prestained and unstained MW standards are available for SDS-PAGE, IEF, 2-D PAGE, and western blotting. For migration charts
with different types of gels, see pages 169 (Mini-PROTEAN® TGX™ gels), 171 (Ready Gel® gels), and 178–179 (Criterion™ gels).
For More Information
Request or download bulletins: 2414, 2998, and 3118
Broad Range
Kaleidoscope
Broad Range
Polypeptide
High Range
High Range
Low Range
Low Range
WesternC™
Dual Color
Unstained
Standard
Dual Xtra
Plugs***
All Blue
Natural
2-D
IEF
17.5–76
Molecular weight 10–250 10–250 2–250 10–250 10–250 10–250 6.9–210 14–97 45–200 7.6–216 6.5–200 14–97 45–200 1.4–26.6 4.45– 10–250
kD
(MW)/pI range, kD kD kD kD kD kD kD kD kD kD kD kD kD kD kD 9.6 pI
4.5–8.5 pI
kD
Number of proteins 10 10 12 10 10 10 8 6 4 7 9 6 5 6 9 7 10
Electrophoresis
SDS-PAGE • • • • • • • • • • • • • • – – •
Accurate MW
• • • • • • – – – – • • • • – – •
estimation
Multicolored • • • • – – – – – • – – – – – – –
Coomassie staining • • • • • • • • • • • • • • • • •
Fluorescent staining – – – – – • – – – – • • • • – • •
2-D electrophoresis – – – – – – – – – – – – – – – • –
IEF – – – – – – – – – – – – – – • – –
Blotting
Monitoring transfer
• • • • • – • • • • – – – – – – –
efficiency
Coomassie staining • • • • • • • • • • • • • • – • •
Fluorescent staining – – – – – • – – – – • • • • – • •
Fluorescent blotting* • • • • • – – – – – – – – – – – –
Immunodetection** • – – – – • – – – – – – – – – – •
Catalog Numbers
Single unit 1610385 1610375 1610377 1610374 1610373 1610363 1610318 1610305 1610309 1610324 1610317 1610304 1610303 1610326 1610310 1610320 1610378
* These standards have fluorescent properties and can be used for fluorescent blotting applications. See bulletin 5723 for details on using Precision Plus Protein™ WesternC™
standards for fluorescent multiplexing. Precision Plus Protein Dual Xtra standards (#1610377) are recommended for fluorescent blot analysis of proteins between 5–250 kD.
** Immunodetection via addition of a Precision Protein™ StrepTactin and horseradish peroxidase (HRP) or StrepTactin and alkaline phosphatase (AP) conjugate, which will bind
to the internal Strep-tags on the proteins.
*** 24 unstained plugs for 2-D gels.
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 157
Protein Electrophoresis
Protein Standards bio-rad.com/proteinstandards
— 2
New and Improved Dual Color Standard
Dual Color Unstained All Blue Kaleidoscope WesternC Dual Xtra
Brighter for easier identification of target proteins
■■
■■
xceptional band sharpness, providing clear confirmation
E 15—
■■
Ready-to-use formulation
158 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Protein Electrophoresis
bio-rad.com/proteinstandards Protein Standards
1 2 3 4 5 6 7 8 9 Individual
Precision Plus Protein
standard with
integrated Strep-tag
sequence
Add
StrepTactin-AP
or -HRP
conjugate
StrepTactin
Western blot detection of 27 kD protein and Precision Plus conjugate binds
Protein WesternC standards using the Immun-Star™ WesternC™
Strep-tag sequence
chemiluminescence detection kit. Maximum sensitivity achievable
with the Immun-Star WesternC kit is in the mid-femtogram range. A gel
run with 5 µl of Precision Plus Protein WesternC standards (lane 1) and
a dilution series of E. coli lysate containing an overexpressed 27 kD
protein (lanes 2–9) was transferred to a nitrocellulose membrane. The
dilutions were: 200, 150, 100, 75, 50, 25, 12, and 6 ng. The blot was
probed with a primary antibody specific for the 27 kD protein, then Add
incubated with StrepTactin-horseradish peroxidase (HRP) and substrate
a secondary antibody conjugated to HRP. After incubation in the
Immun-Star WesternC detection solution for 5 min, the blot was Colorless substrate
imaged using the ChemiDoc™ XRS system. comes in contact
For More Information with StrepTactin-AP
Web: bio-rad.com/pppstandards or -HRP conjugate
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 159
Protein Electrophoresis
Protein Standards bio-rad.com/proteinstandards
Ordering Information
Catalog # Description Price, U.S. $
1610363 Precision Plus Protein Unstained Standards, 1,000 µl, 100 applications 91.00
1610396 Precision Plus Protein Unstained Standards Value Pack, 5 x 1,000 µl, 500 applications 395.00
1610373 Precision Plus Protein All Blue Standards, 500 µl, 50 applications 97.00
1610393 Precision Plus Protein All Blue Standards Value Pack, 5 x 500 µl, 250 applications 425.00
1610374 Precision Plus Protein Dual Color Standards, 500 µl, 50 applications 125.00
1610394 Precision Plus Protein Dual Color Standards Value Pack, 5 x 500 µl, 250 applications 515.00
1610375 Precision Plus Protein Kaleidoscope Standards, 500 µl, 50 applications 135.00
1610395 Precision Plus Protein Kaleidescope Standards Value Pack, 5 x 500 µl, 250 applications 550.00
1610385* Precision Plus Protein WesternC Pack, 250 µl WesternC standard, 125 µl HRP conjugate, 50 applications 200.00
1610398* Precision Plus Protein WesternC Pack, Value Pack, 5 x 250 µl WesternC standard, 5 x 840.00
125 µl HRP conjugate, 250 applications
1610376* Precision Plus Protein WesternC Standards, 250 µl, 50 applications 155.00
1610399* Precision Plus Protein WesternC Standards Value Pack, 5 x 250 µl, 250 applications 640.00
1610377 Precision Plus Protein Dual Xtra Standards, 500 µl, 50 applications 135.00
1610397 Precision Plus Protein Dual Xtra Standards Value Pack, 5 x 500 µl, 250 applications 555.00
StrepTactin Conjugates for Precision Plus Protein Standards
1610380 Precision Protein StrepTactin-HRP Conjugate, 300 µl, 150 applications 90.00
1610381 Precision Protein StrepTactin-HRP Conjugate, 125 µl, 50 applications 31.00
1610382 Precision Protein StrepTactin-AP Conjugate, 300 µl, 150 applications 90.00
Precision Plus Protein Standard Plugs
1610378 Precision Plus Protein Standard Plugs, unstained, 24 applications 120.00
Clarity Western ECL Substrate
1705060 Clarity Western ECL Substrate, 200 ml 135.00
1705061 Clarity Western ECL Substrate, 500 ml 255.00
* Note that StrepTactin (-HRP or -AP) conjugate is needed for colorimetric or chemiluminescence blots.
160 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Protein Electrophoresis
bio-rad.com/proteinstandards Protein Standards
Kaleidoscope™ Standards
BSA
Calibrated MWs of Kaleidoscope Standards
Protein Color MW Prestained
Myosin Blue 216,000 Carbonic
b-Galactosidase Magenta 132,000 anhydrase
BSA Green 78,000
Carbonic anhydrase Violet 45,700 Soybean trypsin
Soybean trypsin inhibitor Orange 32,500 inhibitor
Lysozyme Purple 18,400
Aprotinin Blue 7,600
Lysozyme
MWs are of representative lots; actual weights may vary. Lot-specific MWs
are included with each vial. Aprotinin
Kaleidoscope
Prestained
Standards
Ordering Information
Catalog # Description Price, U.S. $
1610324 Kaleidoscope Prestained Standards, broad range, 500 µl 120.00
Standards have a shelf life of 3 years at –20ºC; shipped at room temperature.
Ordering Information
Catalog # Description Price, U.S. $
1610318 Prestained SDS-PAGE Standards, broad range, 500 µl 105.00
1610305 Prestained SDS-PAGE Standards, low range, 500 µl 86.00
1610309 Prestained SDS-PAGE Standards, high range, 500 µl 94.00
Standards have a shelf life of 3 years at –20ºC; shipped at room temperature.
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 161
Protein Electrophoresis
Protein Standards bio-rad.com/proteinstandards
Ordering Information
Catalog # Description Price, U.S. $
1610317 SDS-PAGE Standards, broad range, 200 µl 115.00
1610304 SDS-PAGE Standards, low range, 200 µl 92.00
1610303 SDS-PAGE Standards, high range, 200 µl 110.00
1610326 Polypeptide SDS-PAGE Standards, 200 µl 85.00
162 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Protein Electrophoresis
bio-rad.com/proteinstandards Protein Standards
IEF Standards
Ordering Information
Catalog # Description Price, U.S. $
1610310 IEF Standards, 250 µl 120.00
5.1
5.5
5.9
6.6
7.0
8.5
5
Protein pI MW, kD
1. Hen egg white conalbumin 6.0, 6.3, 6.6 76 21,500
2. Bovine serum albumin (BSA) 5.4, 5.5, 5.6 66 6
17,500
3. Bovine muscle actin 5.0, 5.1 43 7
4. Rabbit muscle GAPDH 8.3, 8.5 36
5. Bovine carbonic anhydrase 5.9, 6.0 31
6. Soybean trypsin inhibitor 4.5 21.5 Migration pattern of 2-D SDS-PAGE standards. First dimension
7. Equine myoglobin 7.0 17.5 separation was performed with 7 cm ReadyStrip™ IPG strips. Second
dimension separation was achieved with the Mini-PROTEAN® II cell.
Ordering Information
Catalog # Description Price, U.S. $
1610320 2-D SDS-PAGE Standards, 500 µl 195.00
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 163
Protein Electrophoresis
Mini-Format 1-D Electrophoresis Systems bio-rad.com/minielectro
8 7 2
cell into a Mini Trans-Blot® electrophoresis transfer cell
for western blotting
3
Gel Casting
Ground glass plates with permanently bonded spacers
■■
164 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Protein Electrophoresis
bio-rad.com/minielectro Mini-Format 1-D Electrophoresis Systems
Specifications
Number of gels 1–4 Total buffer volume for 2 gels 700 ml
Precast gels Mini-PROTEAN and Ready Gel Total buffer volume for 4 gels 1,000 ml
Handcast gels Cast using Mini-PROTEAN spacer plates Typical run times for SDS-PAGE 35–45 min (at 200 V constant)
Cassette size (W x L) Precast: 10.0 x 8.3 cm Recommended power supply See Power Supplies Selection Guide, p. 153
Glass plate size (W x L) Short plate: 10.1 x 7.3 cm Dimensions (W x L x H) 12.0 x 16.0 x 18.0 cm
Spacer plate: 10.1 x 8.2 cm Weight 1 kg (2.2 lb)
Ordering Information
Catalog # Description Price, U.S. $
1658000FC ini-PROTEAN Tetra Cell, 10-well, 0.75 mm thickness; 4-gel system includes 5 combs, 1 set of glass
M 935.00
plates (5 short plates and 5 spacer plates), 2 casting stands, 4 casting frames, sample loading guide,
electrode assembly, companion running module, tank, lid with power cables, mini cell buffer dam
1658001FC Mini-PROTEAN Tetra Cell, 10-well, 1.0 mm thickness; 4-gel system includes 5 combs, 1 set of glass 935.00
plates (5 short plates and 5 spacer plates), 2 casting stands, 4 casting frames, sample loading guide,
electrode assembly, companion running module, tank, lid with power cables, mini cell buffer dam
1658002FC* Mini-PROTEAN Tetra Cell, 10-well, 0.75 mm thickness; 2-gel system includes 5 combs, 1 set of glass 740.00
plates (5 short plates and 5 spacer plates), casting stand, 2 casting frames, sample loading guide,
electrode assembly, tank, lid with power cables, mini cell buffer dam
1658003FC* Mini-PROTEAN Tetra Cell, 10-well, 1.0 mm thickness; 2-gel system includes 5 combs, 1 set of glass 740.00
plates (5 short plates and 5 spacer plates), casting stand, 2 casting frames, sample loading guide,
electrode assembly, tank, lid with power cables, mini cell buffer dam
1658004 Mini-PROTEAN Tetra Cell for Mini Precast Gels, 4-gel system includes electrode assembly, companion 615.00
running module, tank, lid with power cables, mini cell buffer dam
1658005* Mini-PROTEAN Tetra Cell for Mini Precast Gels, 2-gel system includes electrode assembly, tank, 470.00
lid with power cables, mini cell buffer dam
1658006FC Mini-PROTEAN Tetra Cell, 10-well, 1.5 mm thickness; 4-gel system includes 5 combs, 1 set of glass 935.00
plates (5 short plates and 5 spacer plates), 2 casting stands, 4 casting frames, sample loading guide,
electrode assembly, companion running module, tank, lid with power cables, mini cell buffer dam
1658007FC* Mini-PROTEAN Tetra Cell, 10-well, 1.5 mm thickness; 2-gel system includes 5 combs, 1 set of glass 740.00
plates (5 short plates and 5 spacer plates), casting stand, 2 casting frames, sample loading guide,
electrode assembly, tank, lid with power cables, mini cell buffer dam
Mini-PROTEAN Tetra Systems
1658025FC Mini-PROTEAN Tetra Cell and PowerPac Basic Power Supply, includes #1658001 and #1645050 1,225.00
1658026FC Mini-PROTEAN Tetra Cell and PowerPac Universal Power Supply, includes #1658001 and #1645070 3,090.00
1658027FC Mini-PROTEAN Tetra Cell and PowerPac HC Power Supply, includes #1658001 and #1645052 1,685.00
1658028FC Mini-PROTEAN Tetra Cell and PowerPac HV Power Supply, includes #1658001 and #1645056 3,040.00
1658029FC Mini-PROTEAN Tetra Cell and Mini Trans-Blot Module, includes #1658001 and #1703935 1,280.00
1658030 Mini-PROTEAN Tetra Cell for Mini Precast Gels and Mini Trans-Blot Module, includes #1658004 and #1703935 985.00
1658033FC Mini-PROTEAN Tetra Cell, Mini Trans-Blot Module, and PowerPac Basic Power Supply, includes #1658001, 1,515.00
#1703935, and #1645050
1658034FC Mini-PROTEAN Tetra Cell for Mini Precast Gels, Mini Trans-Blot Module, and PowerPac Basic Power Inquire
Supply, includes #1658004, #1703935, and #1645050
1658035FC Mini-PROTEAN Tetra Cell, Mini Trans-Blot Module, and PowerPac HC Power Supply, includes #1658001, 2,060.00
#1703935, and #1645052
1658036 Mini-PROTEAN Tetra Cell for Mini Precast Gels, Mini Trans-Blot Module, and PowerPac HC Power Supply, 1,850.00
includes #1658004, #1703935, and #1645052
continues
™ ™
The catalog numbers above with an “FC” suffix come with a 10% TGX Stain-Free FastCast kit (1610182). To place an order without a FastCast kit,
remove the "FC" suffix from the catalog number.
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 165
Protein Electrophoresis
Mini-Format 1-D Electrophoresis Systems bio-rad.com/minielectro
Ordering Information
Description 0.75 mm 1.0 mm 1.5 mm Price, U.S. $
Casting Modules**
5-Well 1658008 1658013 1658019 405.00
9-Well 1658009 1658014 1658020 405.00
10-Well 1658010 1658015 1658021 405.00
15-Well 1658011 1658016 1658022 405.00
Prep/2-D Well 1658012 1658017 1658023 405.00
IPG Well — 1658018 1658024 405.00
Mini-PROTEAN Combs (5) for Hand Casting with Glass Plates
5-Well 1653352 1653357 1653363 38.00
9-Well 1653353 1653358 1653364 38.00
10-Well 1653354 1653359 1653365 38.00
15-Well 1653355 1653360 1653366 38.00
Prep/2-D + 1 Reference Well 1653356 1653361 1653367 38.00
IPG Well — 1653362 1653368 38.00
166 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Protein Electrophoresis
bio-rad.com/minielectro Mini-Format 1-D Electrophoresis Systems
Specifications
Number of gels 1–12
Precast gels Mini-PROTEAN and Ready Gel®
Handcast gels Cast using Mini-PROTEAN 3 spacer plates and the Mini-PROTEAN 3 multi-casting chamber
Cassette size (W x L) 10.0 x 8.3 cm
Gel thickness 0.5, 0.75, 1.0, or 1.5 mm (precast gels are available only in 1.0 mm)
Total buffer volume 3.4–4.4 L
Typical running conditions 200 V constant, 600 mA, 120 W maximum
Cooling Built-in cooling coil attaches easily to external refrigerated circulator (circulator must be purchased separately;
recommended flow rate 10–15 L/min, recommended cooling capacity ≥250 W at 20°C)
Recommended power supply PowerPac™ HC
Dimensions (W x L x H) 16.2 x 41.5 x 15.0 cm
Weight 5 kg (11 lb)
Ordering Information
Catalog # Description Price, U.S. $
1654100 ini-PROTEAN 3 Dodeca Cell, includes electrophoresis tank with built-in cooling coil, lid with power cables,
M 4,165.00
6 electrophoresis clamping frames, 2 buffer dams, drain line, 2 gel releasers
1654101 Mini-PROTEAN 3 Dodeca Cell with Multi-Casting Chamber, same as #1654100 with multi-casting chamber, 4,540.00
15 separation sheets, 8 acrylic blocks, tapered luer connector, stopcock valve
1655132 Mini-PROTEAN 3 Dodeca Cell and 6-Row AnyGel Stand, includes #1654100 and #1655131 4,365.00
Replacement Parts and Accessories
1654102 Replacement Electrophoresis Clamping Frame 245.00
1653149 Replacement Gaskets, for electrophoresis clamping frame, green, 2 48.00
1654103 Lower Electrode Assembly with Platinum Wire 485.00
1654104 Replacement Drain Line 110.00
1654105 Replacement Cooling Coil, includes connector tubing 150.00
1652948 Replacement Power Cables, for lid 73.00
1653320 Gel Releasers, 5 30.00
1653130 Mini Cell Buffer Dams, 2 (compatible with Mini-PROTEAN Tetra cell, Mini-PROTEAN 3 Dodeca cell, 16.00
and the discontinued Mini-PROTEAN 3 cell)
1655131 AnyGel Stand, 6-row, holds 6 PROTEAN gels, 12 Criterion gels, or 18 Ready Gel mini gels 295.00
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 167
Protein Electrophoresis
Mini-Format 1-D Electrophoresis Systems bio-rad.com/minielectro
168 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Mini-PROTEAN TGX Protein Electrophoresis
bio-rad.com/minielectro
Precision Plus Protein™ Unstained Mini-Format 1-D Electrophoresis Systems
7.5% 10% 12% 4–15% 4–20% Any kD™
10
Mini-PROTEAN Precast Gel Migration Charts
TGX and SFX with Broad Range Unstained Standards Tris-Tricine
7.5% 10% 12% 4–15% 4–20% 8–16% Any kD 16.5% 10–20%
200 200
200 200 200
200 200 116
97.5 116
97.4
116 66
116 97.4 116 66
97.4 97.4
116 116
45
97.4 66 97.4 45
66
66
45 66 31
45
31
66 45
45 31
31 21.5
21.5
31
21.5 14.4
45 31 14.4
21.5 21.5 14.4 6.5
6.5 6.5
21.5 14.4
14.4
31 6.5
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 169
Protein Electrophoresis
Mini-Format 1-D Electrophoresis Systems bio-rad.com/minielectro
Ordering Information
Applications Guide
1658100 Mini-PROTEAN Gel Instruction Manual and Application Guide, online 3.00
170 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Protein Electrophoresis
bio-rad.com/minielectro Mini-Format 1-D Electrophoresis Systems
Size, kD
(Tris/glycine/SDS buffer)
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 171
Protein Electrophoresis
Mini-Format 1-D Electrophoresis Systems bio-rad.com/minielectro
pH Size, kD Size, bp
(IEF cathode buffer, (Tris/glycine/SDS buffer) (TBE buffer)
IEF anode buffer)
Ordering Information
172 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Protein Electrophoresis
bio-rad.com/minielectro Mini-Format 1-D Electrophoresis Systems
Ordering Information
Catalog # Description Price, U.S. $
Premixed Buffers for Tris-HCI Gels
1610737 2x Laemmli Sample Buffer, 30 ml 14.00
1610747 4x Laemmli Sample Buffer, 10 ml 15.00
1610738 2x Native Sample Buffer, 30 ml 16.00
1610732 10x Tris/Glycine/SDS, 1 L 28.00
1610734 10x Tris/Glycine, 1 L 28.00
1610772 10x Tris/Glycine/SDS, 5 L cube 94.00
1610771 10x Tris/Glycine, 5 L cube 94.00
Premixed Buffers for Tris-Tricine Gels for Peptides
1610739 2x Tricine Sample Buffer, 30 ml 14.00
1610744 10x Tris/Tricine/SDS, 1 L 86.00
Premixed Buffers for IEF Gels
1610763 IEF Sample Buffer, 30 ml 14.00
1610761 10x IEF Anode Buffer, 250 ml 11.00
1610762 10x IEF Cathode Buffer, 250 ml 57.00
Premixed Buffers for Zymogram Gels
1610764 Zymogram Sample Buffer, 30 ml 16.00
1610765 10x Zymogram Renaturation Buffer, 125 ml 13.00
1610766 10x Zymogram Development Buffer, 125 ml 14.00
Premixed Buffers for TBE and TBE-Urea Gels
1610767 5x Nucleic Acid Sample Loading Buffer, 10 ml 20.00
1610768 2x TBE-Urea Sample Buffer, 30 ml 56.00
1610770 10x Tris/Boric Acid/EDTA (TBE), 5 L cube 110.00
Accessories
1610992 Ready Gel Key Knife, free upon request with Ready Gel purchase 6.00
■■
Facilitates loading IPG strips on both large format gels
and Criterion gel sizes using a front clamp that slants the
gel to an ideal angle (can also be used to load tube gels)
■■
A stair-step design and clear clamps so gels
are clearly visible while casting and loading
The single-row AnyGel stand is ideal for processing a few
gels at a time. It accommodates one PROTEAN gel,
two Criterion gels, or three Mini-PROTEAN mini gels.
AnyGel Single-Row Stand with Mini-PROTEAN Cassettes
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Protein Electrophoresis
Mini-Format 1-D Electrophoresis Systems bio-rad.com/minielectro
Ordering Information
Catalog # Description Price, U.S. $
Mini-PROTEAN Empty Cassettes and Combs
4560003 Mini-PROTEAN Empty Cassettes, 10-well, 50 225.00
4560005 Mini-PROTEAN Empty Cassettes, 12-well, 50 225.00
4560006 Mini-PROTEAN Empty Cassettes, 15-well, 50 225.00
4560001 Mini-PROTEAN Empty Cassettes, IPG-well, 7 cm IPG strip, 50 225.00
4560013 Mini-PROTEAN Combs, 10-well, 50 54.00
4560015 Mini-PROTEAN Combs, 12-well, 50 54.00
4560016 Mini-PROTEAN Combs, 15-well, 50 54.00
4560011* Mini-PROTEAN Combs, IPG, 50 54.00
AnyGel Stands and Accessories
1654131 AnyGel Stand, single-row, holds 1 PROTEAN gel, 2 Criterion gels, or 3 Mini-PROTEAN or Ready Gel mini gels 78.00
1655131 AnyGel Stand, 6-row, holds 6 PROTEAN gels, 12 Criterion gels, or 18 Mini-PROTEAN or Ready Gel mini gels 295.00
1654132 Replacement Clamps, 2 33.00
AnyGel Stands and Electrophoresis Cells
1655134 PROTEAN Plus Dodeca Cell (100/120 V) and Two 6-Row AnyGel Stands, Inquire
includes #1654150 and two #1655131
1655135 PROTEAN Plus Dodeca Cell (220/240 V) and Two 6-Row AnyGel Stands, Inquire
includes #1654151 and two #1655131
1655133 Criterion Dodeca Cell and 6-Row AnyGel Stand, includes #1654130 and #1655131 3,050.00
1656020 Criterion Cell and Single-Row AnyGel Stand, includes #1656001 and #1654131 765.00
Mini-PROTEAN 3 Multi-Casting Chambers
1654110** Mini-PROTEAN 3 Multi-Casting Chamber, includes 15 separation sheets, 8 acrylic blocks, 415.00
tapered luer connector, stopcock valve (order glass plates and combs separately)
1654111** Mini-PROTEAN 3 Multi-Casting Chamber, 0.75 mm, includes 15 sets of glass plates 600.00
1654112** Mini-PROTEAN 3 Multi-Casting Chamber, 1.0 mm, includes 15 sets of glass plates 600.00
1654113** Mini-PROTEAN 3 Multi-Casting Chamber, 1.5 mm, includes 15 sets of glass plates 600.00
1654116** Mini-PROTEAN 3 Multi-Casting Chamber, 0.5 mm, includes 15 sets of glass plates 459.00
Mini-PROTEAN 3 Multi-Casting Chamber Accessories
1654114 Acrylic Blocks, 6.0 mm, 8 94.00
1654115 Separation Sheets, 15 59.00
1653320 Gel Releasers, 5 30.00
1652913 Replacement Gaskets, for Mini-PROTEAN 3 multi-casting chamber, includes 3' of tubing 44.00
continues
174 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Protein Electrophoresis
bio-rad.com/midielectro Midi-Format 1-D Electrophoresis Systems
Ordering Information
Catalog # Description Price, U.S. $
Combs**, *** and Glass Plates for 2-D Electrophoresis
1653308 Short Plates, 5 25.00
1653310 Spacer Plates with 0.75 mm Integrated Spacers, 5 66.00
1653311 Spacer Plates with 1.0 mm Integrated Spacers, 5 55.00
1653312 Spacer Plates with 1.5 mm Integrated Spacers, 5 55.00
1653362 Mini-PROTEAN Comb, IPG well, 1.0 mm 38.00
1653368 Mini-PROTEAN Comb, IPG well, 1.5 mm 38.00
1653356 Mini-PROTEAN Comb, prep/2-D well, 0.75 mm 38.00
1653361 Mini-PROTEAN Comb, prep/2-D well, 1.0 mm 38.00
1653367 Mini-PROTEAN Comb, prep/2-D well, 1.5 mm 38.00
Model 485 Gradient Former
1654120 Model 485 Gradient Former, 40–175 ml, includes body with valve stem and tubing connection kit 850.00
1654122 Model 485 Gradient Former and Mini-PROTEAN 3 Multi-Casting Chamber, 1,220.00
ncludes #1654120 and #1654110
Model 485 Gradient Former Accessories
1652006 Exponential Piston, for Model 385 and Model 485 gradient formers 300.00
1652007 Gradient Pouring Needles, 2 66.00
1652008 Tubing Connection Kit, includes stopcock, luer taper coupling, tubing (1/8" ID, 3'), Y-connector 44.00
* Catalog #4560011 is an IPG well only, no reference well. If a protein standard is needed on gel, order Precision Plus Protein
standard plugs #1610378.
** Order combs separately (see combs for use with glass plates on page 166) in the Mini-PROTEAN Tetra cell section.
*** For multi-well comb configurations, refer to page 166.
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Ordering Information
Catalog # Description Price, U.S. $
Criterion Cell and Systems
1656001 Criterion Cell, includes electrophoresis buffer tank, lid with power cables, 3 sample loading guides 585.00
(12+2 well, 18-well, 26-well)
1656019 Criterion Cell and PowerPac Basic Power Supply, 100–120/220–240 V, includes #1656001 and #1645050 1,100.00
1656020 Criterion Cell and Single-Row AnyGel Stand, includes #1656001 and #1654131 765.00
Criterion Cell and Blotter Systems
1656024 Criterion Cell/Plate Blotter System, includes #1656001 and #1704070 1,365.00
1656025 Criterion Cell/Wire Blotter System, includes #1656001 and #1704071 1,365.00
Replacement Parts
1656002 Criterion Replacement Electrophoresis Buffer Tank, with lower electrodes 360.00
1656003 Criterion Replacement Lid, with upper electrodes 280.00
1656004 Criterion Replacement Upper Electrode, includes prestrung platinum wire 110.00
1656005 Criterion Replacement Lower Electrode, includes prestrung platinum wire 185.00
1652948 Replacement Power Cables, for lid 73.00
1654131 AnyGel Stand, single-row, holds 1 PROTEAN gel, 2 Criterion gels, or 3 Mini-PROTEAN or Ready Gel mini gels 78.00
Criterion Dodeca Cell
1654130 Criterion Dodeca Cell, includes electrophoresis buffer tank with built-in cooling coil, lid with power cables 3,050.00
1654138 Criterion Dodeca Cell and PowerPac HC Power Supply, includes #1654130 and #1645052 3,905.00
1654139 Criterion Dodeca Cell and PowerPac Universal Power Supply, includes #1654130 and #1645070 5,360.00
1655133 Criterion Dodeca Cell and 6-Row AnyGel Stand, includes #1654130 and #1655131 3,050.00
Replacement Parts
1654104 Replacement Drain Line 110.00
1654135 Lower Electrode with Platinum Wire 595.00
1654136 Replacement Cooling Coil, includes connector tubing 99.00
1654137 Replacement Lid 1,205.00
1652948 Replacement Power Cables, for lid 73.00
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in individual units: 1–2 gels can be run in the Criterion Dodeca stainers:
page 182.
cell (page 175) and 1–12 gels can be run in the high- Criterion staining
throughput Criterion Dodeca cell (page 176). trays: page 182.
Standards:
■■
Fast run times and 12-month shelf life for Criterion™ TGX™ gels page 157.
■■
oom temperature storage and 12-month shelf life for
R Electrophoresis
stains:
Criterion XT Bis-Tris gels page 199.
■■
ormats that run up to 26 samples on a single gel
F
without reducing sample volume or sacrificing speed
■■
patented* cassette design including an integral
A Integral buffer chamber
upper buffer chamber that never leaks and requires
Gel type, expiration date,
no tools to open catalog, and lot numbers
■■
ample wells that are outlined and numbered for
S
Outlined and
easy loading numbered wells
■■
Multichannel pipet–compatible combs
Criterion TGX Precast Gels Available Chemistries
These precast gels use the Laemmli buffer system and have IEF pH 3–10, 5–8
a 12-month shelf life. And they maintain cooler temperatures
Stain-Free 10%, 4–20%, 8–16% Tris-HCl
at elevated voltages, allowing for reduced run times.
TBE 5%, 10%, 15%, 4–20%
■■
Run times as short as 20 min TBE-urea 5%, 10%, 15%
■■
ransfer times as short as 7 min with the
T TGX 7.5%, 10%, 12%, 18% resolving
Trans-Blot® Turbo™ system 4–15%, 4–20%, 8–16%, 10–20% linear gradient
Any kD™
■■
12-month shelf life TGX Stain-Free™ 7.5%, 10%, 12%, 18%, 4–15%, 4–20%
■■
Laemmli format, no special buffers required 8–16%, 10–20% linear gradient
Any kD
■■
Integrated upper buffer chamber
Tris-acetate 7%, 3–8% resolving
Criterion TGX Precast Gel Specifications Tris-HCI 5%, 7.5%, 10%, 12.5%, 15%, 18% resolving
4–15%, 4–20%, 8–16%, 10–20%, 10.5–14%
Gel dimensions (W x L) 13.3 x 8.7 cm; 1.0 mm thick
linear gradient
Cassette dimensions (W x L) 15.0 x 10.6 cm; 5.3 mm thick
Tris-Tricine 16.5%, 10–20%
Cassette material Styrene copolymer
XT (Bis-Tris) 10%, 12%, 4–12% resolving
Comb material Polycarbonate
Zymogram 10% with gelatin, 12.5% with casein
Gel storage conditions Store flat at 4°C; do not freeze
Shelf life at recommended 12 months
temperature*
Recommended sample buffer Laemmli sample buffer: 62.5 mM
(dilute 1:1 with sample) Tris-HCl, pH 6.8, 2% SDS, 25%
glycerol, 0.01% bromophenol blue
Recommended running buffer 25 mM Tris, 192 mM glycine,
(Tris/glycine/SDS) 0.1% SDS, pH 8.3
Run times 42–50 min at 200 V
20–26 min at 300 V
* From date of manufacture.
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200 200
116
200 116 97.5 200 200 200
97.5 66 200 116
200 200 116 116
97.5 97.5 97.5
116 66 45
97.5 66
116 66 66
116 116 97.5
45 31 45
97.5 66 97.5
45 45
66
66 31
66 31 21.5
45 45
31 31
45
14.4 31 21.5
Criterion
7.5% TGX/TGX
10% Stain-Free
12% gels. 18% 4–15% 4–20% 8–16% 10–20% Any kD™
7.5% 10% 12% 18% 4–15% 4–20% 8–16% 10–20% Any kD
Ordering Information
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Ordering Information
12+2-Well*, ** 18-Well 26-Well* Prep+2-Well** IPG+1-Well**
Description 45 μl 50 μl 15 μl 800 μl 11 cm IPG Strip Price, U.S. $
Criterion XT Tris-Acetate Gels
7% Resolving Gel 3450135 3450136† 3450137† — — 16.00
3–8% Resolving Gel 3450129 3450130 3450131 — 3450133† 16.00
Criterion Tris-HCl Gels
5% Resolving Gel 3450001 3450002 3450003† — — 15.00
7.5% Resolving Gel 3450005 3450006 3450007 3450008 — 15.00
10% Resolving Gel 3450009 3450010 3450011 3450012† 3450101 15.00
12.5% Resolving Gel 3450014 3450015 3450016 3450017† 3450102 15.00
15% Resolving Gel 3450019 3450020 3450021 3450022† — 15.00
18% Resolving Gel 3450023 3450024 3450025 3450026† — 15.00
4–15% Linear Gradient 3450027 3450028 3450029 3450030† 3450103 15.00
4–20% Linear Gradient 3450032 3450033 3450034 3450035 3450104 15.00
8–16% Linear Gradient 3450037 3450038 3450039 3450040† 3450105 15.00
10–20% Linear Gradient 3450042 3450043 3450044 3450045† 3450107 15.00
10.5–14% Linear Gradient 3459949 3459950 3459951 — 3450106 15.00
Criterion Stain Free Gels
10% Tris-HCI Gel 3451012 3451018 — — — 17.00
4–20% Tris-HCl Gel 3450412 3450418 3450426 — — 17.00
8–16% Tris-HCl Gel 3458162 — 3458166 — 3458161 17.00
Criterion Tris-Tricine Gels
16.5% Tris-Tricine 3450063 3450064 3450065† 3450066† — 16.00
10–20% Tris-Tricine 3450067 3450068 3450069 — — 16.00
Criterion IEF Gels
pH 3–10 3450071† 3450072† 3450073† — — 38.00
pH 5–8 — 3450076† — — — 57.00
Criterion Zymogram Gels
10% Zymogram Gel with Gelatin 3450079† 3450080† 3450081† — — 19.00
12.5% Zymogram Gel with Casein 3450082† 3450083† 3450084† — — 33.00
Criterion TBE Gels
5% TBE Gel 3450047 3450048 3450049 — — 16.00
10% TBE Gel 3450051 3450052 3450053 — — 16.00
15% TBE Gel 3450055† 3450056 3450057 — — 16.00
4–20% TBE Gel 3450059† 3450060† 3450061† — — 16.00
Criterion TBE-Urea Gels
5% TBE-Urea Gel — 3450086† — — — 19.00
10% TBE-Urea Gel 3450088† 3450089† 3450090† — — 16.00
15% TBE-Urea Gel 3450091 3450092 3450093† — — 16.00
Criterion Empty Cassettes
1.0 mm thick, 10 sets 3459901 3459902 3459903 3459904 3459906 81.00
Loading Guides
Criterion Sample Loading Guide†† 1656006 1656007 1656008 — — 27.00
continues
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See Also
Ordering Information
Premixed running Catalog # Description Price, U.S. $
buffers: page 192.
Criterion XT Buffers and Reagents
1610788*** XT MOPS Running Buffer, 20x, 500 ml 63.00
1610789*** XT MES Running Buffer, 20x, 500 ml 63.00
1610790 XT Tricine Running Buffer, 20x, 500 ml 63.00
1610791 XT Sample Buffer, 4x, 10 ml 13.00
1610792 XT Reducing Agent, 20x, 1 ml 23.00
1610793*** XT MOPS Buffer Kit, includes 500 ml of 20x XT MOPS running buffer, 10 ml of 4x XT sample buffer, 96.00
1 ml of 20x XT reducing agent
1610796*** XT MES Buffer Kit, includes 500 ml of 20x XT MES running buffer, 10 ml of 4x XT sample buffer, 96.00
1 ml of 20x XT reducing agent
1610797 XT Tricine Buffer Kit, includes 500 ml of 20x XT Tricine running buffer, 10 ml of 4x XT sample buffer, 96.00
1 ml of 20x XT reducing agent
Application Guide
4110001 Criterion Precast Gel Application Guide, available online Inquire
* Multichannel pipet compatible. ** Includes reference well(s), 15 μl. *** Purchase of this product is accompanied by a limited license under
U.S. patents 6,143,154; 6,096,182; 6,059,948; 5,578,180; 5,922,185; 6,162,338; and 6,783,651 and corresponding foreign patents.
† Please allow up to 2 weeks for delivery. †† U.S. patent 5,656,145.
Criterion™ Accessories
Empty Cassettes 1
AnyGel Stands 7
AnyGel stands are convenient for storing glass plates of any
size gel. They are available as single-row or six-row stands. 4
See the Mini-PROTEAN® precast gel section, page 168. 3
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Ordering Information
Catalog # Description Price, U.S. $
AnyGel Stands and Accessories
1654131 AnyGel Stand, single-row, holds 1 PROTEAN gel, 2 Criterion gels, or 3 Mini-PROTEAN or Ready Gel mini gels 78.00
1655131 AnyGel Stand, 6-row, holds 6 PROTEAN gels, 12 Criterion gels, or 18 Mini-PROTEAN or Ready Gel mini gels 295.00
1654132 Replacement Clamps, 2 33.00
Criterion Staining Trays
3459921 Criterion Staining/Blotting Trays, with lids, 2 25.00
3459920 Criterion Staining/Blotting Trays, with lids, 12 215.00
Dodeca Stainers, Accessories, and Replacement Parts
1653400 Dodeca Stainer, large, 100–240 V, includes 13 trays (12 clear, 1 white), 12 tray attachments, shaking rack, 4,600.00
solution tank, lid with shaker motor, shaker control unit, gel clip
1653401 Dodeca Stainer, small, 100–240 V, includes 13 trays (12 clear, 1 white), 12 Criterion tray attachments, 4,255.00
shaking rack, solution tank, lid with shaker motor, shaker control unit, gel clip
1653403 Dodeca Stainer and Dodeca Silver Stain Kit, large, 100–240 V, includes large Dodeca stainer (#1653400), 4,785.00
Dodeca silver stain kit for the large tank (#1610480)
1653404 Dodeca Stainer and Dodeca Silver Stain Kit, small, 100–240 V, includes small Dodeca stainer (#1653401), 4,340.00
Dodeca silver stain kit for the small tank (#1610481)
1653405 Dodeca Stainer and Bio-Safe Coomassie Stain Kit, large, 100–240 V, includes large Dodeca stainer 4,735.00
(#1653400) and staining solution for a large tank, sufficient for up to 12 large format gels
1653406 Dodeca Stainer and Bio-Safe Coomassie Stain Kit, small, 100–240 V, includes small Dodeca stainer 4,445.00
(#1653401) and staining solution for a small tank, sufficient for up to 12 large format gels
1653407 Dodeca Stainer and SYPRO Ruby Protein Gel Stain Kit, large, 100–240 V, includes large Dodeca stainer 6,295.00
(#1653400) and SYPRO staining solution for a large tank, sufficient for up to 12 large format gels
1653408 Dodeca Stainer and SYPRO Ruby Protein Gel Stain Kit, small, 100–240 V, includes small Dodeca stainer 6,005.00
(#1653401) and SYPRO staining solution for a small tank, sufficient for up to 12 large format gels
1653429 Storage Box, large, holds up to 4 gels on large staining trays 160.00
1653430 Storage Box, small, holds up to 4 gels on small staining trays 155.00
1653416 Dodeca Stainer Tray, small, replacement, 2 125.00
1653420 Dodeca Stainer White Development Tray, small 45.00
1653422 Dodeca Stainer Shaking Rack, small, replacement 925.00
1653423 Dodeca Stainer Solution Tank, large, replacement 420.00
1653424 Dodeca Stainer Solution Tank, small, replacement 360.00
1653425 Dodeca Stainer Lid with Shaker Motor, 100–240 V, replacement, fits both tank sizes 950.00
1653426 Dodeca Stainer Lid without Shaker Motor, replacement, fits both tank sizes 180.00
1653428 Dodeca Stainer Shaker Control Unit, replacement 280.00
1610480 Dodeca Silver Stain Kit, large, for use with large Dodeca stainer (#1653400), for 12 large gels 755.00
1610481 Dodeca Silver Stain Kit, small, for use with small Dodeca stainer (#1653401), for 12 large or 24 midi gels 540.00
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Specifications
PROTEAN II xi (16 cm) PROTEAN II xi (20 cm) PROTEAN II XL (20 cm)
Number of gels 1–4 1–4 2
Gel size (W x L) 16 x 16 cm (handcast) 16 x 20 cm (handcast) 18.3 x 20 cm (handcast)
Glass plate size (W x L)
Inner plate 20 x 16 cm 20 x 20 cm 20 x 20 cm
Outer plate 20 x 18.3 cm 20 x 22.3 cm 20 x 22.3 cm
Spacer length 18.3 cm 22.3 cm 22.3 cm
Typical upper buffer volume 350 ml 350 ml 350 ml
Typical lower buffer volume 1.8 L 1.2 L 1.2 L
Typical run times for SDS-PAGE*
Without cooling 4 hr 5 hr 5 hr
With cooling 2.5 hr 3.5 hr 3.5 hr
Recommended power supply PowerPac™ HV or PowerPac HV or PowerPac HV or
PowerPac Universal PowerPac Universal PowerPac Universal
* For voltage and current settings for e
lectrophoresis applications, see page 153.
Ordering Information
Catalog # Description Price, U.S. $
PROTEAN II xi Cells, for 16 x 16 cm* Gels
1651801 PROTEAN II xi Cell, without spacers and combs** 1,615.00
1651802 PROTEAN II xi Cell, 1.5 mm spacers (4), 15-well combs (2) 1,660.00
1651803 PROTEAN II xi Cell, 1.0 mm spacers (4), 15-well combs (2) 1,710.00
1651804 PROTEAN II xi Cell, 0.75 mm spacers (4), 15-well combs (2) 1,660.00
PROTEAN II xi Cells, for 16 x 20 cm Gels
1651811 PROTEAN II xi Cell, without spacers and combs** 1,675.00
1651812 PROTEAN II xi Cell, 1.5 mm spacers (4), 15-well combs (2) 1,670.00
1651813 PROTEAN II xi Cell, 1.0 mm spacers (4), 15-well combs (2) 1,720.00
1651814 PROTEAN II xi Cell, 0.75 mm spacers (4), 15-well combs (2) 1,670.00
PROTEAN II XL Cells, for 18.3 x 20 cm Gels*, Compatible with ReadyStrip IPG Strips
1653188 PROTEAN II XL Cell, wide-format, 1.0 mm, spacers (4), IPG 2-D combs (2) 1,930.00
1653189 PROTEAN II XL Cell, wide-format, 1.5 mm, spacers (4), IPG 2-D combs (2) 1,980.00
1653190 PROTEAN II XL Cell, wide-format, 2.0 mm, spacers (4), IPG 2-D combs (2) 1,930.00
PROTEAN II IPG Conversion Kits, for 2-D (to Convert xi to XL)***
1651815 PROTEAN II xi Cell 2-D Conversion Kit, converts PROTEAN II xi cell into a tube gel IEF 2-D system, 700.00
2 tube gel adaptors, 24 glass tubes (1.5 mm diameter, 180 mm length), gaskets, grommets, stoppers
1653183 PROTEAN II xi Cell IPG Conversion Kit, 1.0 mm spacers 680.00
1653186 PROTEAN II xi Cell IPG Conversion Kit, 1.5 mm spacers 680.00
1653184 PROTEAN II xi Cell IPG Conversion Kit, 2.0 mm spacers 680.00
1651834 PROTEAN II xi Basic Unit with Casting Stand, includes central cooling core, lower buffer chamber, lid with 1,420.00
cables, leveling bubble; combine with an IPG conversion kit for a complete 18.3 cm wide format system
* All cells include central cooling core with gaskets, lower buffer chamber, lid with cables, 2 sets of glass plates, sandwich
clamps (4), upper buffer dam, casting stand with gaskets, leveling bubble, instructions, and alignment card.
** Select spacers and combs from page 186–187.
*** All PROTEAN II xi cell IPG conversion kits include 2 sets of IPG clamps, 2 sets of 20 x 20 cm glass plates, IPG spacers (4),
IPG 2-D combs (2), IPG central cooling core gaskets (2), casting stand gaskets (2), and alignment card.
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* Requires a refrigerated circulating bath and operation at 4°C for optimal results.
xi XL
PROTEAN II xi and XL (IPG) component comparison:
1. The 4 mm xi and 13 mm XL clamp notches.
2. The 181 mm xi and 198 mm XL central cooling core gaskets.
3. The 153 mm xi and 184 mm XL combs.
4. The 19 mm xi and 8 mm XL spacers.
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Ordering Information
Catalog # Description Price, U.S. $
PROTEAN II xi Accessories for Running Gels
1651901 PROTEAN II xi Sandwich Clamps, 16 cm set (1 left, 1 right) 135.00
1651902 PROTEAN II xi Sandwich Clamps, 20 cm set (1 left, 1 right) 135.00
1651913 PROTEAN II xi Replacement Gaskets, for central cooling core, 2 59.00
PROTEAN II XL Accessories for Running Gels
1651835 PROTEAN II XL Sandwich Clamps, IPG set (1 left, 1 right) 140.00
1653182 PROTEAN II XL Replacement Gaskets, for central cooling core, 2 71.00
PROTEAN II xi and XL Accessories for Running Gels
1651806 Central Cooling Core, includes 2 gaskets 980.00
1651807 Buffer Tank 560.00
1651808 Cell Lid, with power cables 245.00
1651909 Upper Buffer Dam 92.00
1005430 PROTEAN II Latch Assembly Kit, for central cooling core 51.00
900768018 Replacement Platinum Wire, cathode, 18" Inquire
900768024 Replacement Platinum Wire, anode, 24" Inquire
PROTEAN II xi and XL Casting Apparatus
1651911 Slab Gel Casting Stand, with gaskets 225.00
1651912 Replacement Gaskets, for casting stand, 2 44.00
PROTEAN II xi Glass Plates*
1651821 Inner Plates, for 16 x 16 cm gels, 16 x 20 cm, 2, for PROTEAN II xi cell only 58.00
1651822 Outer Plates, for 16 x 16 cm gels, 18.3 x 20 cm, 2, for PROTEAN II xi cell only 58.00
PROTEAN II xi Specialty Glass Plates
1651825** Frosted Inner Plates, for agarose gels, 16 x 20 cm, 2, for PROTEAN II xi cell only 145.00
1651826** Frosted Inner Plates, for agarose gels, 20 x 20 cm, 2, for PROTEAN II xi cell only 145.00
1651832 Notched Inner Plate, for double-up procedures, 16 x 16 cm gel, 16 cm bevel length, for PROTEAN II xi cell only 120.00
1651833 Notched Inner Plate, for double-up procedures, 16 x 20 cm gel, 16 cm bevel length, for PROTEAN II xi cell only 120.00
PROTEAN II xi and XL Glass Plates, for 16 x 20 or 18.3 x 20 cm gels*
1651823 Inner Plates, 20 x 20 cm, 2 58.00
1651824 Outer Plates, 22.3 x 20 cm, 2 58.00
Catalog # Description
PROTEAN II XL Spacers, IPG Strip Format (Set of 4)
1651836 20 cm Spacers, 1.0 mm 98.00
1653181 20 cm Spacers, 1.5 mm 110.00
1651837 20 cm Spacers, 2.0 mm 100.00
* One gel sandwich consists of 1 outer plate, 1 inner plate, and 2 spacers.
** E
ach comb is 15.2 cm long. All combs except the 2-D combs produce sample wells that are 25 mm deep.
The well depth of the 2-D comb is 8 mm. The well depth of all standard combs can be converted from 25 mm to
10 mm with comb conversion screws (#1651859).
continues
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Ordering Information
Catalog # Description Price, U.S. $
PROTEAN II xi Multi-Cell*
1651951 PROTEAN II xi Multi-Cell, includes 3 central cooling cores with gaskets, buffer tank, lid with power cables, 4,580.00
1 upper buffer dam, PROTEAN II xi multi-casting chamber with accessories, leveling bubble
1651956 PROTEAN II xi Multi-Cell 2-D Conversion Kit, for proper cooling, includes 2 cooling coils and manifold 615.00
(required for 2-D electrophoresis applications)
PROTEAN II XL Multi-Cell, Wide Format, Compatible with ReadyStrip IPG Strips*, **
1653176 PROTEAN II XL Multi-Cell, wide format, 1.0 mm 6,775.00
1653177 PROTEAN II XL Multi-Cell, wide format, 1.5 mm 6,775.00
1653178 PROTEAN II XL Multi-Cell, wide format, 2.0 mm 6,775.00
* The PROTEAN i12 IEF system is required for first-dimension IEF with the PROTEAN II xi or XL multi-cell; see page 202.
** Includes catalog #1651951, #1651956, and 3 PROTEAN II xi cell IPG conversion kits of desired thickness. Order appropriate
spacers, plates, clamps, combs, and accessories for your application (pages 185–187).
Ordering Information
Catalog # Description Price, U.S. $
1652025 ROTEAN II xi Multi-Gel Casting Chamber, includes casting chamber, sealing plate, silicone gasket,
P 765.00
15 separation sheets, 4 acrylic blocks, 10 xi alignment cards, tapered luer connector, leveling bubble
1652024 PROTEAN II XL Multi-Gel Casting Chamber, includes casting chamber, sealing plate, silicone gasket, 865.00
15 separation sheets, 4 acrylic blocks, 10 XL alignment cards, tapered luer connector, leveling bubble
Accessories
1651957 Acrylic Blocks, 4 135.00
1651958 Separation Sheets, 15 120.00
1652026 Sealing Gaskets, 3 63.00
1652029 PROTEAN II xi Alignment Cards, 2 22.00
1651840 PROTEAN II XL Alignment Cards, 2 58.00
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* Designed to run IPG and tube gel samples; not recommended for 1-D applications.
** U.S. patent 6,451,193.
Ordering Information
Catalog # Description Price, U.S. $
PROTEAN Plus Dodeca Cells and Systems
1654150 PROTEAN Plus Dodeca Cell, 100/120 V, includes electrophoresis buffer tank with built-in ceramic Inquire
cooling core, lid, buffer recirculation pump with tubing, 2 gel releasers
1654140 PROTEAN Plus Dodeca Cell (100/120 V) and PowerPac HC Power Supply, includes #1654150 Inquire
and #1645052
1654142 PROTEAN Plus Dodeca Cell (100/120 V) and PowerPac Universal Power Supply, Inquire
includes #1654150 and #1645070
1654144 PROTEAN Plus Dodeca Cell (100/120 V), Trans-Blot Plus Cell, and PowerPac Universal Power Supply, Inquire
includes #1654150, #1703990, and #1645070
1655134 PROTEAN Plus Dodeca Cell (100/120 V) and Two 6-Row AnyGel Stands, includes #1654150 and Inquire
two #1655131
1654151 PROTEAN Plus Dodeca Cell, 220/240 V, includes electrophoresis buffer tank with built-in ceramic Inquire
cooling core, lid, buffer recirculation pump with tubing, 2 gel releasers
1654141 PROTEAN Plus Dodeca Cell (220/240 V) and PowerPac HC Power Supply, includes #1654151 Inquire
and #1645052
1654143 PROTEAN Plus Dodeca Cell (220/240 V) and PowerPac Universal Power Supply, Inquire
includes #1654151and #1645070
1654145 PROTEAN Plus Dodeca Cell (220/240 V), Trans-Blot Plus Cell, and PowerPac Universal Power Supply, Inquire
includes #1654151, #1703990, and #1645070
1655135 PROTEAN Plus Dodeca Cell (220/240 V) and Two 6-Row AnyGel Stands, includes #1654151 and Inquire
two #1655131
Accessories and Replacement Parts
1654158 Recirculation Pump, 100/120 V 900.00
1654159 Recirculation Pump, 220/240 V 900.00
1654153 Replacement Tubing Kit, for tank with stopcock drain port installed at base of tank 335.00
1654152 Replacement Old Tubing Kit, for tank without stopcock drain port installed at base of tank 389.00
1654154 Replacement Gasket Assembly 160.00
1654155 Replacement Electrode Card, anode 1,035.00
1654156 Replacement Electrode Card, cathode 285.00
1654157 Replacement Lid 685.00
1654166 Manifold Tubing, required for precast gels and PROTEAN II plates, 11 pieces 720.00
1654167 Buffer Exhaust Tubing 82.00
1653320 Gel Releasers, 5 30.00
1652948 Replacement Power Cables, for lid 73.00
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 189
Protein Electrophoresis
Large-Format Vertical Electrophoresis Systems bio-rad.com/largeelectro
Gel Clip
The gel clip facilitates the handling of large-format gels
and eliminates gel breakage by minimizing direct hands-on
gel manipulation. The gel clip gently but securely clamps
along one edge of a gel, distributing the weight evenly so Using the gel clip to clamp onto a gel
that the gel can be easily lifted without tearing. (PROTEAN Plus precast gel shown).
190 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Protein Electrophoresis
bio-rad.com/largeelectro Large-Format Vertical Electrophoresis Systems
Ordering Information
Catalog # Description Price, U.S. $
PROTEAN Plus Combs
1654176 2-D Comb with 1 Reference Well, 20 cm, 1.0 mm 25.00
1654177 2-D Comb with 1 Reference Well, 20 cm, 1.5 mm 25.00
1654178 2-D Comb with 1 Reference Well, 20 cm, 2.0 mm 25.00
1654179 2-D Comb with 1 Reference Well, 25 cm, 1.0 mm 25.00
1654180 2-D Comb with 1 Reference Well, 25 cm, 1.5 mm 25.00
1654181 2-D Comb with 1 Reference Well, 25 cm, 2.0 mm 25.00
PROTEAN Plus Hinged Spacer Plates
1654170 Hinged Spacer Plates, for 20 x 20.5 cm gels, 1.0 mm, 1 set 110.00
1654171 Hinged Spacer Plates, for 20 x 20.5 cm gels, 1.5 mm, 1 set 110.00
1654172 Hinged Spacer Plates, for 20 x 20.5 cm gels, 2.0 mm, 1 set 110.00
1654173 Hinged Spacer Plates, for 25 x 20.5 cm gels, 1.0 mm, 1 set 110.00
1654174 Hinged Spacer Plates, for 25 x 20.5 cm gels, 1.5 mm, 1 set 97.00
1654175 Hinged Spacer Plates, for 25 x 20.5 cm gels, 2.0 mm, 1 set 97.00
PROTEAN Plus Multi-Casting Chamber
1654160 PROTEAN Plus Multi-Casting Chamber, includes casting chamber, sealing plate, silicone gasket, 1,045.00
tapered luer connector, leveling bubble, 15 separation sheets, 8 acrylic blocks (order glass hinged spacer
plates and combs separately)
Accessories for PROTEAN Plus Multi-Casting Chamber
1654165 Separation Sheets, for PROTEAN Plus multi-casting chamber, 15 93.00
1654161 Acrylic Block, 1.5 mm 37.00
1654162 Acrylic Block, 3 mm 41.00
1654163 Acrylic Block, 6 mm 45.00
1654164 Acrylic Block, 12 mm 48.00
1653320 Gel Releasers, 5 30.00
Gel Clip
1653414 Gel Clip, holds any gel size 41.00
Model 495 Gradient Former
1654121 Model 495 Gradient Former, 100–1,500 ml, includes body with valve stem and tubing connection kit 1,150.00
1654123 Model 495 Gradient Former and PROTEAN Plus Multi-Casting Chamber, includes #1654121 and #1654160 2,165.00
Accessories for Model 495 Gradient Former
1652005 Exponential Piston, for Model 395 and Model 495 gradient formers 485.00
1652008 Tubing Connection Kit, includes stopcock, luer taper coupling, tubing (1/8" ID, 3'), Y-connector 44.00
PROTEAN II xi Plate Washer/Holder
1651991* PROTEAN II xi Plate Washer System, includes 2 plate holders, washing tank, lid, 486.00
1 bottle of Bio-Rad cleaning concentrate
1651992* PROTEAN II xi Plate Holder 155.00
1610722 Bio-Rad Cleaning Concentrate, 50x, 1 kg 59.00
* Not compatible with Mini-PROTEAN 3 short plates and spacer plates or with PROTEAN Plus hinged spacer plates.
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 191
Protein Electrophoresis
Buffers and Reagents for Protein Electrophoresis bio-rad.com/proteinreagents
Ordering Information
Catalog # Description Price, U.S. $
Premixed Protein Sample Loading Buffers
1610737 2x Laemmli Sample Buffer, 30 ml 14.00
1610747 4x Laemmli Sample Buffer, 10 ml 15.00
1610738 Native Sample Buffer, 30 ml 16.00
1610739 Tricine Sample Buffer, 30 ml 14.00
1610763 IEF Sample Buffer, 30 ml 14.00
1610764 Zymogram Sample Buffer, 30 ml 16.00
1610791 XT Sample Buffer, 4x, 10 ml 13.00
192 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Protein Electrophoresis
bio-rad.com/proteinreagents Buffers and Reagents for Protein Electrophoresis
Ordering Information
Catalog # Description Price, U.S. $ Catalog # Description Price, U.S. $
Premixed Protein Running Buffers Premixed Nucleic Acid Running Buffers
1610732 10x Tris/Glycine/SDS, 1 L 28.00 1610733 10x Tris/Boric Acid/EDTA (TBE), 37.00
1610772 10x Tris/Glycine/SDS, 5 L cube 94.00 1L
1610734 10x Tris/Glycine, 1 L 28.00 1610770 10x Tris/Boric Acid/EDTA (TBE), 110.00
1610771 10x Tris/Glycine, 5 L cube 94.00 5 L cube
1610744 10x Tris/Tricine/SDS, 1 L 86.00 1610741 10x Tris/Boric Acid/EDTA (TBE), 57.00
1610761 10x IEF Anode Buffer, 250 ml 11.00 extended range, 1 L
1610762 10x IEF Cathode Buffer, 250 ml 57.00 1610743 50x Tris/Acetic Acid/EDTA (TAE), 84.00
1610765 10x Zymogram Renaturation 13.00 1L
Buffer, 125 ml
1610766 10x Zymogram Development 14.00
Buffer, 125 ml
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 193
Protein Electrophoresis
Buffers and Reagents for Protein Electrophoresis bio-rad.com/proteinreagents
Ordering Information
Catalog # Description Price, U.S. $
1610170 GX FastCast Acrylamide Starter Kit, 7.5%
T 50.00
1610171 TGX FastCast Acrylamide Kit, 7.5% 180.00
1610172 TGX FastCast Acrylamide Starter Kit, 10% 50.00
1610173 TGX FastCast Acrylamide Kit, 10% 180.00
1610174 TGX FastCast Acrylamide Starter Kit, 12% 50.00
1610175 TGX FastCast Acrylamide Kit, 12% 180.00
1610180 TGX Stain-Free FastCast Acrylamide Starter Kit, 7.5% 55.00
1610181 TGX Stain-Free FastCast Acrylamide Kit, 7.5% 200.00
1610182 TGX Stain-Free FastCast Acrylamide Starter Kit, 10% 55.00
1610183 TGX Stain-Free FastCast Acrylamide Kit, 10% 200.00
1610184 TGX Stain-Free FastCast Acrylamide Starter Kit, 12% 55.00
1610185 TGX Stain-Free FastCast Acrylamide Kit, 12% 200.00
194 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Protein Electrophoresis
bio-rad.com/proteinreagents Buffers and Reagents for Protein Electrophoresis
Gel-Casting Reagents
Acrylamide
■■
Acrylamide powders — acrylamide and bis-acrylamide
powders allow adjustment of concentration and
acrylamide/bis ratio
■■
Premixed acrylamide/bis powders — premeasured
amounts allow stock solutions from 30–50% to be
prepared directly in the bottle by adding the indicated
amount of water
■■
crylamide solutions — ready to use and available
A
in two concentrations (30% and 40%) and in three Acrylamide/Cross-Linker Ratio by Application
acrylamide/bis ratios (19:1, 29:1, and 37.5:1) Ratio %C Common Applications
19:1 5 DNA sequencing
Pore Size Determination: %T and %C 29:1 3.3 Protein separation
Polyacrylamide gels are described in terms of two parameters 37.5:1 2.6 Protein separation
that determine pore size: total monomer concentration (%T)
and weight percentage of cross-linker (%C).
the molecule of interest. For help in determining the best %T
grams acrylamide + grams cross-linker and %C for your application, refer to the Precast Gels sections
%T = x 100%
total volume, ml on pages 168 and 177. Examples of migration patterns of
proteins on gels of different compositions can be found on
grams cross-linker page 169 (Mini-PROTEAN® TGX™ precast gels) and 178
%C = x 100%
grams acrylamide + grams cross-linker (Criterion™ precast gels), or contact Bio-Rad Technical Support.
For More Information
By varying these two parameters, the pore size of the gel can Web: bio-rad.com/acrylamide
be optimized to give the best separation and resolution for Request or download bulletins: 1156 and 1866
Ordering Information
Description Price, U.S. $ Price, U.S. $
Acrylamide Solutions* 500 ml 2 x 500 ml
30% acrylamide/bis, 19:1 1610154 59.00 1610155 120.00
30% acrylamide/bis, 29:1 1610156 59.00 1610157 120.00
30% acrylamide/bis, 37.5:1 1610158 59.00 1610159 120.00
40% acrylamide/bis, 19:1 1610144 71.00 1610145 135.00
40% acrylamide/bis, 29:1 1610146 71.00 1610147 135.00
40% acrylamide/bis, 37.5:1 1610148 71.00 1610149 135.00
40% acrylamide 1610140 51.00 1610141 105.00
2% bis solution 1610142 53.00 1610143 94.00
Catalog # Description
1615100 SDS-PAGE Reagent Starter Kit, includes 100 g acrylamide, 5 g bis, 5 ml TEMED, 10 g ammonium persulfate 61.00
1632091 ReadyPrep Proteomics Grade Water, 500 ml 40.00
* Store acrylamide solutions at 4°C. All other reagents should be stored at room temperature, dry, and away from direct sunlight.
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 195
Protein Electrophoresis
Buffers and Reagents for Protein Electrophoresis bio-rad.com/proteinreagents
Ordering Information
Catalog # Description Price, U.S. $
Crosslinkers
1610200 Bis Crosslinker, 5 g 16.00
1610201 Bis Crosslinker, 50 g 51.00
1610142 2% Bis Solution, 500 ml 53.00
1610143 2% Bis Solution, 2 x 500 ml 94.00
1610202 PDA Crosslinker, 10 g 130.00
1610620 DATD Crosslinker, 25 g 84.00
Catalysts
1610800 TEMED*, 5 ml (hazardous shipping charges may apply) 17.00
1610801 TEMED, 50 ml 55.00
1610700 Ammonium Persulfate (APS)*, 10 g 14.00
1610501 Riboflavin-5'-Phosphate*, 10 g 29.00
* For longer shelf life, store desiccated at room temperature.
IEP/IEF Agaroses
Standard low –mr agarose — with high strength, clarity, Zero –mr agarose — this agarose is specific for IEF. It has
and low –mr value, this agarose is recommended for all no detectable electroendosmosis and is recommended for
standard immunoelectrophoresis applications. IEF of very high MW proteins or complexes that are subject
to varying degrees of molecular sieving in polyacrylamide
gels. Agarose IEF and post-run processing can be
completed more quickly than polyacrylamide gel IEF.
For More Information
Web: bio-rad.com/IEP/IEFAgaroses
196 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Protein Electrophoresis
bio-rad.com/proteinreagents Buffers and Reagents for Protein Electrophoresis
Ordering Information
Catalog # Description Price, U.S. $
1620100 Standard Low –mr Agarose, 100 g 165.00
1620102 Standard Low –mr Agarose, 500 g 620.00
1620022 Zero –mr Agarose, 10 g 160.00
All reagents should be stored at room temperature, dry, and away from direct sunlight.
Accessory Reagents
Tracking Dyes
Ordering Information
Catalog # Description Price, U.S. $
1610404 Bromophenol Blue, 10 g 40.00
1610423 Xylene Cyanole FF, 25 g 120.00
All dyes and stains should be stored at room temperature, dry, and away from direct sunlight.
Ordering Information
Catalog # Description Price, U.S. $
1610301 SDS (Sodium Dodecyl Sulfate), 100 g 33.00
1610302 SDS (Sodium Dodecyl Sulfate), 1 kg 210.00
1610416 SDS Solution, 10% (w/v), 250 ml 45.00
1610418 SDS Solution, 20% (w/v), 1 L 120.00
1706531 Tween 20, EIA grade, 100 ml 53.00
1610407 Triton X-100 Detergent, 500 ml 35.00
1610460 CHAPS*, 1 g 60.00
1610781 10% Tween 20, for easy pipetting, 1 L 36.00
* Store desiccated at 4°C. All other reagents should be stored at room temperature, dry, and away from direct sunlight.
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 197
Protein Electrophoresis
Buffers and Reagents for Protein Electrophoresis bio-rad.com/proteinreagents
PROTEAN i12 IEF Bio-Lyte carrier ampholytes, supplied as aqueous solutions, 3/10
3/10
system: are blended to give a complete range of isoelectric 3/5
3/5
Bio-Lyte Ampholyte
page 202.
points for linear pH gradients. Bio-Lyte 3/10, 3/5, and 4/6
4/6
8/10 ampholytes extend from approximately pH 3.5–9.5 5/7
5/7
units on the acidic and basic ends. All other ranges are 5/8
5/8
within 0.1–0.2 pH units of their specified range. Bio-Lyte 6/8
6/8
ampholytes are also used as IPG buffers. For ready-to-use 7/9
7/9
ampholytes, see ReadyStrip™ IEF buffers (page 206). 8/10
8/10
3 4 5 6 7 8 9 10
pH range of each Bio-Lyte ampholyte.
Ordering Information
Catalog # Description Price, U.S. $ Catalog # Description Price, U.S. $
1631112 Bio-Lyte 3/10 Ampholyte, 40%, 10 ml 275.00 1631192 Bio-Lyte Ampholyte, 40%, 10 ml 275.00
1631113 Bio-Lyte 3/10 Ampholyte, 40%, 25 ml 645.00 1631193 Bio-Lyte 5/8 Ampholyte, 40%, 25 ml 645.00
1631132 Bio-Lyte 3/5 Ampholyte, 20%, 10 ml 275.00 1631162 Bio-Lyte 6/8 Ampholyte, 40%, 10 ml 275.00
1631142 Bio-Lyte 4/6 Ampholyte, 40%, 10 ml 275.00 1631163 Bio-Lyte 6/8 Ampholyte, 40%, 25 ml 645.00
1631143 Bio-Lyte 4/6 Ampholyte, 40%, 25 ml 645.00 1631172 Bio-Lyte 7/9 Ampholyte, 40%, 10 ml 275.00
1631152 Bio-Lyte 5/7 Ampholyte, 40%, 10 ml 275.00 1631182 Bio-Lyte 8/10 Ampholyte, 20%, 10 ml 275.00
1631153 Bio-Lyte 5/7 Ampholyte, 40%, 25 ml 645.00
Cleaning Concentrate
Bio-Rad’s cleaning concentrate is a moderately alkaline detergent that cleans by solubilization and emulsification.
It is ideal for cleaning glass plates and other laboratory equipment and is harmless to skin and clothing.
Ordering Information
Catalog # Description Price, U.S. $
1610722 Bio-Rad Cleaning Concentrate, 50x, 1 kg 59.00
Ordering Information
Catalog # Description Price, U.S. $
1610752 Gel Drying Solution, 1 L 46.00
198 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Protein Electrophoresis
bio-rad.com/proteinstains Protein Stains
Protein Stains
Bio-Rad offers visible and fluorescent gel stains to accommodate your needs for sensitivity, linearity, and mass
spectrometry compatibility.
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 199
Protein Electrophoresis
Protein Stains bio-rad.com/proteinstains
Ordering Information
Catalog # Description Price, U.S. $
QC Colloidal Coomassie Stain
1610803 QC Colloidal Coomassie Stain, 1 L 86.00
Bio-Safe Coomassie Stain
1610786 Bio-Safe Coomassie Stain, 1 L 80.00
1610787 Bio-Safe Coomassie Stain, 5 L 310.00
Coomassie Brilliant Blue R-250 Staining and Destaining Solutions
1610435 Coomassie Brilliant Blue R-250 Staining Solutions Kit, includes 1 L Coomassie Brilliant Blue R-250 135.00
staining solution, 2 x 1 L Coomassie Brilliant Blue R-250 destaining solution
1610436 Coomassie Brilliant Blue R-250 Staining Solution, 1 L 52.00
1610437 Coomassie Brilliant Blue R-250 Staining Solution, 4 x 1 L 215.00
1610438 Coomassie Brilliant Blue R-250 Destaining Solution, 1 L 39.00
1610439 Coomassie Brilliant Blue R-250 Destaining Solution, 4 x 1 L 160.00
Coomassie Stain Powders
1610400 Coomassie Brilliant Blue R-250, 10 g 20.00
1610406 Coomassie Brilliant Blue G-250, 10 g 36.00
Silver Stains
Ordering Information
Catalog # Description Price, U.S. $
Silver Stain Plus Kit
1610449 Silver Stain Plus Kit, includes fixative enhancer concentrate, silver complex solution, reduction moderator 225.00
solution, image development reagent, development accelerator reagent, stains 13 full size or 40 mini gels
1610448 Development Accelerator Reagent, 50 g 25.00
1610461 Fixative Enhancer Concentrate*, 1 L 61.00
1610462 Silver Complex Solution*, 100 ml 29.00
1610463 Reduction Moderator Solution*, 100 ml 44.00
1610464 Image Development Reagent*, 100 ml 56.00
* Hazardous shipping charges may apply.
200 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Protein Electrophoresis
bio-rad.com/proteinstains Protein Stains
Ordering Information
Catalog # Description Price, U.S. $
Bio-Rad Silver Stain Kit*, **
1610443 Silver Stain Kit, includes oxidizer concentrate, silver reagent concentrate, silver stain developer, 195.00
stains 20 full-size or 48 mini gels
1610450 Silver Stain Developer, 115 g 23.00
1610447 Silver Stain Developer, 4 x 115 g 96.00
1610444 Oxidizer Concentrate, 480 ml 66.00
1610445 Silver Reagent Concentrate, 480 ml 66.00
* Hazardous shipping charges may apply.
** The Bio-Rad silver stain kit and components should be stored at 4°C.
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 201
Protein Electrophoresis
2-D Electrophoresis bio-rad.com/2Dsystems
Ordering Information
Catalog # Description Price, U.S. $
Oriole Fluorescent Gel Stain
1610495 Oriole Fluorescent Gel Stain, 1x solution, 200 ml 82.00
1610496 Oriole Fluorescent Gel Stain, 1x solution, 1 L 185.00
1610497 Oriole Fluorescent Gel Stain, 1x solution, 5 L 845.00
Flamingo Fluorescent Gel Stain
1610490 Flamingo Fluorescent Gel Stain, 10x solution, 20 ml 67.00
1610491 Flamingo Fluorescent Gel Stain, 10x solution, 100 ml 195.00
1610492 Flamingo Fluorescent Gel Stain, 10x solution, 500 ml 930.00
SYPRO Ruby Protein Gel Stain
1703126 SYPRO Ruby Protein Gel Stain, 1x solution, 200 ml 91.00
1703125 SYPRO Ruby Protein Gel Stain, 1x solution, 1 L 320.00
1703138 SYPRO Ruby Protein Gel Stain, 1x solution, 5 L 1,105.00
2-D Electrophoresis
IEF is primarily used as the first dimension of separation in 2-D analysis; 2-D electrophoresis is used to separate
complex protein samples based on pI and MW. IEF separations can be performed using two techniques: either with an
IPG strip with ampholytes covalently bound to the gel or with carrier ampholytes that move through the gel to generate
a pH gradient. Bio-Rad offers products for both techniques. Bio-Rad’s first-dimension products are compatible with
second-dimension SDS-PAGE systems in mini, midi, and large formats. For more information, see page 151.
For preparative IEF products see page 213.
Learn More about the Technology For More Information
Web: bio-rad.com/tech/2delectro Web: bio-rad.com/2dworkflow
■■
btain better quality data with less experimental risk —
O 12
one irregular sample cannot compromise the entire run 3
9 8
6 4
11
10 7
5
PROTEAN i12 IEF system components:
1. PROTEAN i12 IEF cell. 9. USB flash drives.
2. Focusing trays with strip retainers. 10. Electrode wicks.
3. Cleaning brushes. 11. Rehydration trays.
4. Pair of electrodes. 12. ReadyPrep™ 2-D starter kit,
5. ReadyStrip IPG Strips. rehydration/sample buffer.
PROTEAN i12 IEF
6. Leveling bubble. 13. Cleaning concentrate.
system touch screen 7. Forceps. 14. Mineral oil.
user interface. 8. Styluses.
202 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Protein Electrophoresis
bio-rad.com/2Dsystems 2-D Electrophoresis
damage the IPG strip. The disposable sample cups For More Information
prevent sample contamination. Web: bio-rad.com/proteani12
Request or download bulletins: 2651, 6097, 6138, 6139, and 6140
Ordering Information
Catalog # Description Price, U.S. $
1646000 ROTEAN i12 IEF System, includes basic unit, 90–240 VAC positive and negative electrode assemblies, 7,
P Inquire
11, and 17 cm focusing trays, 1 pack each of 7, 11, and 17 cm rehydration/equilibration trays, 2 pairs of forceps,
2 packs of electrode wicks for gel-side down and gel-side up applications, mineral oil, 2 cleaning brushes,
cleaning concentrate, 2 USB flash drives, 3 styluses, pH 3–10 ReadyStrip IPG strips (7, 11, and 17 cm lengths),
rehydration/sample buffer, leveling bubble, and instruction manual. All 13, 18, and 24 cm trays and cup loading
accessories can be purchased separately
1646001 PROTEAN i12 IEF Cell, includes PROTEAN i12 IEF cell, 90–240 VAC basic unit, positive and negative electrode Inquire
assemblies, and 3 styluses. Focusing trays and other accessories sold separately
PROTEAN i12 IEF System Accessories
1646107 i12 7 cm Focusing Tray, includes 2 IPG strip retainers 80.00
1646111 i12 11 cm Focusing Tray, includes 2 IPG strip retainers 80.00
1646113 i12 13 cm Focusing Tray, includes 2 IPG strip retainers 80.00
1646117 i12 17 cm Focusing Tray, includes 2 IPG strip retainers 80.00
1646118 i12 18 cm Focusing Tray, includes 2 IPG strip retainers 80.00
1646124 i12 24 cm Focusing Tray, includes 2 IPG strip retainers 80.00
1654035 i12 7 cm Rehydration/Equilibration Tray, includes lids, 25 120.00
1654025 i12 11 cm Rehydration/Equilibration Tray, includes lids, 25 120.00
1646313 i12 13 cm Rehydration/Equilibration Tray, includes lids, 25 200.00
1654015 i12 17 cm Rehydration/Equilibration Tray, includes lids, 25 150.00
1654041 i12 18 cm Rehydration/Equilibration Tray, includes lids, 25 240.00
1654043 i12 24 cm Rehydration/Equilibration Tray, includes lids, 25 160.00
1646040 IPG Strip Retainers, 2 18.00
1646020 i12 Sample Cup Holder, includes 25 sample cups 36.00
1646021 i12 Sample Cups, 25 26.00
1646030 Gel-Side Up Electrode Wicks, 100 31.00
continues
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 203
Protein Electrophoresis
2-D Electrophoresis bio-rad.com/2Dsystems
Ordering Information
Catalog # Description Price, U.S. $
PROTEAN i12 IEF System Accessories (cont.)
1646031 Gel-Side Down Electrode Wicks, 500 31.00
1646012 Negative Electrode Assembly 610.00
1646011 Positive Electrode Assembly 610.00
1646010 Electrode Assembly Pair, includes 1 positive and 1 negative electrode assembly 1,100.00
1654072 Cleaning Brushes, 2 24.00
1610722 Cleaning Concentrate, 1 L 59.00
1646060 USB Flash Drive, 2 54.00
1646050 Stylus, 3 60.00
1632129 Mineral Oil, 500 ml 27.00
1654070 Forceps, 1 50.00
Tray Specifications
IPG Strip Length
7 cm 11 cm 17 cm 18 cm 24 cm
Focusing Trays
Electrode distance 6.5 cm 10.2 cm 16.2 cm 17.1 cm 22.7 cm
Total strip length accommodated 8.2 cm 12.1 cm 18.1 cm 20.1 cm 25.3 cm
ReadyStrip IPG strip length 7.9 cm 11.8 cm 17.8 cm 19.0 cm 24.7 cm
Rehydration/Equilibration Trays
Total strip length accommodated 8.0 cm 12.7 cm 18.6 cm 20.4 cm 25.3 cm
Maximum volume 6.8 ml 9.6 ml 14.2 ml 16.0 ml 19.0 ml
Load
protein
Cup Loading Tray for the PROTEAN IEF System sample
Cup loading expands the versatility and range of
applications for first-dimension IEF using the PROTEAN IEF
cell. This loading method can improve focusing results, Sample
Cup
especially for proteins with pIs in the extreme pH ranges. cup
loading
well
Load up to 150 µl of sample with easy-to-use disposable
sample cups. Moveable electrodes provide the flexibility to IPG strip
204 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Protein Electrophoresis
bio-rad.com/2Dsystems 2-D Electrophoresis
Ordering Information
Catalog # Description Price, U.S. $
Trays
1654030 7 cm Focusing Tray with Lid 380.00
1654020 11 cm Focusing Tray with Lid 405.00
1654010 17 cm Focusing Tray with Lid 440.00
1654040 18 cm Focusing Tray with Lid 475.00
1654042 24 cm Focusing Tray with Lid 690.00
Cup Loading Tray*
1654050 Cup Loading Tray, includes 1 tray base, 1 pair movable electrodes, 1 pack each of large and small replacement cups 1,220.00
1654055 Cup Loading Tray with Forceps 1,199.00
Accessories
1654071 Electrode Wicks, precut, 500 12.00
1654080 Thermal Printer, 100 V, includes cable and power adaptor 990.00
1702412 Thermal Printer Paper, 10 rolls 149.35
1654051 Large Replacement Cups, 150 µl, 120 135.00
1654052 Small Replacement Cups, 100 µl, 120 130.00
1654053 Replacement Movable Electrodes, 1 pair 270.00
1654054 Replacement Cup Loading Tray Base 770.00
* The cup loading tray is not intended or designed for active or passive rehydration of IPG strips. Use the appropriate
rehydration/equilibration tray that matches your IPG strip’s length.
lengths and in a wide selection of pH gradients, with are preprinted to ReadyPrep 2-D
starter kit: page 207.
1, 3, or 7 pH units per strip. Shorter strips are useful indicate anode end
(+) and pH range; in
for method development, while longer strips provide
addition, a barcode
the best separation possible with higher protein loads. is printed on the
ReadyStrip IPG strips are thoroughly tested for quality and 24 cm strip.
performance to deliver 2-D gel-to-gel reproducibility.
Design Features
Relative Focusing Power
Stringent gel length tolerances of ±2 mm
■■
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 205
Protein Electrophoresis
2-D Electrophoresis bio-rad.com/2Dsystems
Ordering Information
pH Range 7 cm Price, U.S. $ 11 cm Price, U.S. $ 17 cm 18 cm Price, U.S. $ 24 cm Price, U.S. $
ReadyStrip IPG Strips, 12 per Package
pH 3–10 1632000 99.00 1632014 125.00 1632007 1632032 140.00 1632042 165.00
pH 3–10 NL* 1632002 99.00 1632016 120.00 1632009 1632033 140.00 1632043 165.00
pH 3–6 1632003 99.00 1632017 120.00 1632010 1632035 140.00 1632045 165.00
pH 4–7 1632001 99.00 1632015 120.00 1632008 1632034 140.00 1632044 165.00
pH 5–8 1632004 99.00 1632018 120.00 1632011 1632036 140.00 1632046 165.00
pH 7–10 1632005 99.00 1632019 120.00 1632012 1632037 140.00 1632047 165.00
ReadyStrip IEF Buffers** and Accessories
1632094 Bio-Lyte 3/10 Ampholyte, 100x, 1 ml 53.00
1632093 ReadyStrip 100x 7–10 Buffer, includes only ampholytes, 1 ml 53.00
1632099 ReadyStrip Instruction Manual, free upon request with ReadyStrip purchases 2.00
* NL, nonlinear gradient.
** Dilute ReadyStrip buffers to 1x in each sample to obtain a final concentration of 0.2% ampholyte.
206 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Protein Electrophoresis
bio-rad.com/2Dsystems 2-D Electrophoresis
Ordering Information
Catalog # Description Price, U.S. $
1632105 eadyPrep 2-D Starter Kit, includes E. coli protein sample and reagents sufficient to rehydrate, focus, and
R 260.00
transfer to second-dimension gels, ReadyStrip IPG strips, precast SDS-PAGE gels, and gel stains not included
1632110 E. coli Protein Sample, lyophilized, 2.7 mg 57.00
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 207
Protein Electrophoresis
2-D Electrophoresis bio-rad.com/2Dsystems
Ordering Information
Catalog # Description Price, U.S. $
1632106 eadyPrep 2-D Starter Kit Rehydration/Sample Buffer, 10 ml, 8 M urea, 2% CHAPS, 50 mM DTT,
R 44.00
0.2% Bio-Lyte 3/10 ampholyte, 0.001% bromophenol blue
1632107 ReadyPrep 2-D Starter Kit Equilibration Buffer I, with DTT, 10 ml, 375 mM Tris-HCl, pH 8.8, 6 M urea, 44.00
2% SDS, 2% DTT
1632108 ReadyPrep 2-D Starter Kit Equilibration Buffer II, without DTT or iodoacetamide, 20 ml, 375 mM Tris-HCl, 37.00
pH 8.8, 6 M urea, 2% SDS
1632091 ReadyPrep Proteomics Grade Water, 500 ml 40.00
1610610 Dithiothreitol (DTT), 1 g 27.00
1610611 Dithiothreitol (DTT), 5 g 81.00
1632101 Tributylphosphine (TBP), 200 mM, 0.6 ml 44.00
1632109 Iodoacetamide, 30 g 145.00
1610731 Urea, 1 kg 76.00
1610719 Tris, 1 kg 84.00
Control Sample
1632110 E. coli Protein Sample, lyophilized, 2.7 mg 57.00
Overlay Agaroses
1632111 ReadyPrep Proteomics Grade Overlay Agarose, 50 ml, 0.5% low melting point agarose in 21.00
1x Tris/glycine/SDS and 0.003% bromophenol blue
1632092 PROTEAN Plus Proteomics Grade Overlay Agarose, 125 ml, 0.75% agarose in 1x Tris/glycine/SDS 71.00
and 0.003% bromophenol blue
Detergent for IEF
1610460 CHAPS, 1 g 60.00
208 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Protein Electrophoresis
bio-rad.com/2Dsystems 2-D Electrophoresis
Ordering Information
Catalog # Description Price, U.S. $
1652960 ini-PROTEAN 2-D Cell, includes tube adaptor, 16 sample reservoirs and stoppers, 50 sample reservoir/capillary
M 1,785.00
tube connectors, 200 capillary tubes with casting tube, tube gel ejector, Mini-PROTEAN II slab cell with electrode
assembly and gaskets, lower buffer chamber, lid with cables, 10 sets of glass plates, 2 clamp assemblies,
two 2-D combs with 1 standard well, four 1.0 mm thick spacers, casting stand with gaskets, leveling bubble
1652961 Mini-PROTEAN Tube Cell, includes tube adaptor, 16 sample reservoirs and stoppers, 50 sample 1,045.00
reservoir/capillary tube connectors, 200 capillary tubes with casting tube, lower buffer chamber,
lid with cables, tube gel ejector
1652965* Mini-PROTEAN Tube Cell Module, same as #1652961, without lower buffer chamber and lid 880.00
Accessories
1652966 Capillary Tubes with Casting Tube, 200 86.00
1652967 Mini 2-D Tube Gel Ejector 130.00
1652968 Mini-PROTEAN Tube Gel Sample Reservoirs, 8 52.00
1652969 Mini-PROTEAN Tube Module Stoppers, 8 45.00
1652970 Mini-PROTEAN Tube Module Tube Connectors, 50 46.00
1645056** PowerPac HV Power Supply, 100–120/220–240 V 2,325.00
* The Mini-PROTEAN tube cell module may be used with the tank and lid of the Mini Trans-Blot cells of older Mini-PROTEAN II or
Mini-PROTEAN 3 systems; the tube cell module is not compatible with the Mini-PROTEAN Tetra system. The Mini-PROTEAN
tube cell (for casting tube gels and performing first-dimension IEF) and the tube cell module (for casting tube gels) are also
available separately.
** Recommended for use with the Mini-PROTEAN 2-D electrophoresis cell.
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 209
Protein Electrophoresis
2-D Electrophoresis bio-rad.com/2Dsystems
Ordering Information
Catalog # Description Price, U.S. $
PROTEAN II xi 2-D Tube Gel Cell*
1651931 PROTEAN II xi 2-D Cell, 1.0 mm, 16 cm 2,300.00
1651932 PROTEAN II xi 2-D Cell, 1.5 mm, 16 cm 2,300.00
1651933 PROTEAN II xi 2-D Cell, 1.0 mm, 20 cm 2,345.00
1651934 PROTEAN II xi 2-D Cell, 1.5 mm, 20 cm 2,345.00
Accessories and Replacement Parts
1651940 Tube Gel Adaptor, with gasket, grommets (4–8 mm OD tubes), stoppers 350.00
1651943 Tube Gel Loading Needles, 18 cm, 22 gauge, blunt tip, luer hub (for casting monomer in small-diameter tubes), 2 84.00
1651944 Tube Gel Extrusion Needles, 9 cm, 26 gauge, beveled tip, luer hub (for removing gels from tubes), 2 84.00
1651947 Replacement Gaskets, for tube gel adaptor, 2 58.00
1651859 PROTEAN II Comb Conversion Screws**, includes 10 comb conversion screws, 10 standard comb screws 33.00
1651827 Beveled Inner Glass Plates, for 2-D tube gel procedures, 16 cm bevel length, 16 x 20 cm, 2, 145.00
for PROTEAN II xi 2-D cells only
1651828 Beveled Inner Glass Plates, for 2-D tube gel procedures, 16 cm bevel length, 20 x 20 cm, 2, 145.00
for PROTEAN II xi 2-D cells only
* Each PROTEAN II xi 2-D cell includes a central cooling core with gaskets, lower buffer chamber, lid with cables, 2 sets of glass
plates (with beveled inner plates), 4 sandwich clamps, twenty-four 180 mm long glass tubes (tube diameter = spacer thickness),
2 tube gel adaptors, 16 grommets, 16 stoppers, two 2-D combs, 4 spacers, upper buffer dam, casting stand with gaskets,
leveling bubble, and instructions. Sandwich clamps are sized to fit the gel length appropriate for the cell (16 cm or 20 cm).
1.0 mm and 1.5 mm indicate the thickness of spacers and combs included with the cell.
** For use with agarose gels. Comb conversion screws convert two PROTEAN II xi combs with standard 25 mm well depth
to combs with a 10 mm well depth. Double-up stacking gels (4 gels/run) cannot be cast simultaneously when comb
conversion screws are used.
210 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Protein Electrophoresis
bio-rad.com/2Dsystems 2-D Electrophoresis
Ordering Information
Catalog # Description Price, U.S. $
Glass Tubes
1653136 1.0 mm ID Glass Tubes, 6.0 mm OD, 180 mm length, 24 140.00
1653137 1.5 mm ID Glass Tubes, 7.5 mm OD, 150 mm length, 24 145.00
1653138 1.5 mm ID Glass Tubes, 7.5 mm OD, 180 mm length, 24 145.00
1653155 2.4 mm ID Glass Tubes, 4.0 mm OD, 160 mm length, 24 125.00
1653150 3.4 mm ID Glass Tubes, 5.0 mm OD, 125 mm length, 24 96.00
1653122 5.0 mm ID Glass Tubes, 7.0 mm OD, 125 mm length, 24 100.00
Model 225 Tube Gel Casting Stand
1652020 Model 225 Tube Gel Casting Stand 600.00
Grommet and Stopper Sets*
1651984 Grommets and Stoppers, for 4–5 mm OD tubes, 12 each 95.00
1651985 Grommets and Stoppers, for 6–7.5 mm OD tubes, 12 each 95.00
* Grommet and stopper sets work with both Model 175 and Model 225 tube gels and the tube gel adaptors for the
PROTEAN II xi cells.
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 211
Protein Electrophoresis
2-D Electrophoresis bio-rad.com/2Dsystems
Ordering Information
Catalog # Description Price, U.S. $
1702975 odel 111 Mini IEF Cell, includes chamber and lid, graphite electrodes, casting tray, 5 glass plates,
M 1,245.00
50 sheets of gel support film for polyacrylamide, 5 sample templates
Accessories
1702980 Graphite Electrodes, 2 83.00
1702981 Mini Casting Tray 295.00
1702982 Glass Plates, 12.5 cm x 6.5 cm x 1.5 mm, 5 84.00
1702983 Gel Support Film for Polyacrylamide, 12.5 x 6.5 cm, 50 sheets 220.00
1702984 Gel Support Film for Agarose, 12.5 x 6.5 cm, 50 sheets 185.00
1702985 Sample Templates, 5 44.00
212 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Protein Electrophoresis
bio-rad.com/prepelectro Preparative Electrophoresis
Preparative Electrophoresis
Preparative electrophoresis devices fractionate and purify nanogram to gram quantities of proteins or nucleic acids via See Also
liquid phase IEF electrophoresis or continuous electroelution from gels. These devices separate and purify molecules Protein sample
according to their molecular mass (using SDS-PAGE or agarose gel electrophoresis), pI (using liquid-phase IEF), or a preparation kits:
page 2.
combination of both molecular mass and pI (using native PAGE or preparative 2-D electrophoresis). These devices include:
■■
odel 491 prep cell and mini prep cell — perform
M
high-resolution separations of proteins and nucleic acids
by continuous-elution gel electrophoresis (PAGE)
■■
odel 422 electro-eluter — elutes macromolecules
M
from gel slices
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 213
Protein Electrophoresis
Preparative Electrophoresis bio-rad.com/prepelectro
See Also Rotofor ® Cell, Mini Rotofor Cell Accessories, and MicroRotofor™
PowerPac HV
power supply:
page 155.
Protein sample Ordering Information
preparation kits:
page 2. Catalog # Description Price, U.S. $
Accessories and Replacement Parts for Rotofor Cell and Mini Rotofor Cell
1702910 Rotofor Starter Kit, includes 10 ml Bio-Lyte ampholytes (pH 3–10), 60 ml syringe, colored protein sample, 245.00
2 vent buttons, one each of the ion exchange membranes, hydrated
1702991 Mini Membrane Cores, for 18 ml focusing chamber, 2 210.00
1702952 Membrane Cores, for 60 ml focusing chamber, 2 210.00
1702953 Repair Kit, includes O-ring kit, 4 ion exchange gaskets, 4 port cover screws, 4 electrolyte chamber screws, 95.00
2 gray port gaskets
1702954 Cooling Finger O-Ring Kit, with 4 O-rings 80.00
1702956 Ion Exchange Membranes, 5 pair 80.00
1702957 Vent Buttons, 8 80.00
1702958 Cooling Finger 545.00
1702960 Sealing Tape, 1 roll, 1 in x 36 yards 35.00
1702964 Harvest Tubing 145.00
1702965 Harvest Box Lid 160.00
1702966 Harvesting Needle Array 225.00
Rotofor Adaptor Kits
1702990 Adaptor Kit, to convert Rotofor cell to mini Rotofor cell, includes mini focusing chamber, 980.00
mini membrane core, 18 ml
1702959 Adaptor Kit, to convert mini Rotofor cell to Rotofor cell, includes focusing chamber, membrane core, 60 ml 915.00
Accessories and Replacement Parts for MicroRotofor Cell
1702804 MicroRotofor Starter Kit, includes Bio-Lyte ampholytes, control protein sample, focusing chamber, 290.00
ion exchange membranes, harvesting tray, syringes
1702810 MicroRotofor Harvesting Trays, 3 87.00
1702820 MicroRotofor Sealing Film, 10 sheets 10.00
1702960 Sealing Tape, 1 roll, 1 in x 36 yards 35.00
1702821 MicroRotofor Focusing Chambers, 3 170.00
1702822 MicroRotofor Cathode Assembly 82.00
1702829 MicroRotofor Anode Assembly 82.00
1702833 MicroRotofor Ion Exchange Membrane Assemblies 65.00
1702835 MicroRotofor Cleaning Brush 20.00
1702836 MicroRotofor Syringes, 3 and 10 ml, 3 each 16.00
1702851 MicroRotofor Needle Assembly 155.00
1702852 MicroRotofor Vacuum Block O-Ring 10.00
1702826 MicroRotofor Electrode Assembly O-Ring/Gasket Kit, electrolyte buffer chamber O-ring and gaskets 70.00
214 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Protein Electrophoresis
bio-rad.com/prepelectro Preparative Electrophoresis
Model 491 Prep Cell and Mini Prep Cell See Also
PowerPac Universal
and PowerPac HV
The Model 491 prep cell and mini prep cells separate power supplies:
page 155.
biomolecules (protein or nucleic acids) by continuous-
elution electrophoresis. Samples are electrophoresed
through a cylindrical gel matrix, where they are separated
Upper
into ring-shaped bands. As individual bands migrate off electrophoresis
the bottom of the gel, they are collected in discrete liquid buffer
To peristaltic pump
fractions. The Model 491 prep cell and the mini prep cell Elution buffer and fraction collector
allow resolution of proteins differing in MW by as little as
2%. With these prep cell systems, you can: Lower electrophoresis
Cooling core buffer
■■
Purify nanogram to milligram quantities of target protein Elution
collection
■■
eparate proteins that differ in MW by as little as 1 kDa
S tube
by using SDS-PAGE Gel tube
■■
eparate proteins by mass and charge with pI differences
S
Protein bands
as little as 0.1 pH units by using native PAGE
■■
eparate large proteins or DNA fragments (up to 18 kb)
S
Patented
by using agarose gel electrophoresis elution
chamber
For More Information
Buffer r ecirculation pump
Web: bio-rad.com/prepPAGE
Request or download bulletins: 1964, 3153, and 3161 Continuous-elution electrophoresis in the Model 491 prep cell.
Samples are electrophoresed through the cylindrical gel matrix where
they are separated into ring-shaped bands. Individual bands migrate
off the bottom of the gel and are collected in discrete liquid fractions
available for assay and characterization.
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 215
Protein Electrophoresis
Preparative Electrophoresis bio-rad.com/prepelectro
Specifications
Model 491 Prep Cell Mini Prep Cell
Sample capacity (mass/volume) 1–500 mg/0.5–15 ml 0.5–1.0 mg/50–500 µl
Gel tube dimensions 28 and 37 mm ID, 14 cm length 7 mm ID, 13 cm length
Cooling Glazed alumina-ceramic tube Not necessary
Electrical limits 500 V, 40 mA, 20 W 500 V, 10 mA, 5 W
(PowerPac™ HV or PowerPac Universal recommended) (PowerPac HV or PowerPac Universal recommended)
Elution buffer flow rate 1 ml/min 0.1 ml/min
Auxiliary equipment required Fraction collector, power supply, peristaltic pump Fraction collector, power supply
Ordering Information
Catalog # Description Price, U.S. $
Model 491 Prep Cells
1702926 Model 491 Prep Cell, 100/120 V, includes buffer recirculation pump, prep cell starter kit with protein standard 5,570.00
1702927 Model 491 Prep Cell, 220/240 V 5,405.00
1702928 Model 491 Prep Cell without Buffer Recirculation Pump 4,920.00
Replacement Parts and Accessories for the Model 491 Prep Cell
1702944 Prep Cell Casting Stand 265.00
1702929 Buffer Recirculation Pump, 100/120 V 960.00
1702930 Buffer Recirculation Pump, 220/240 V 880.00
1702932 Small Gel Tube Assembly, 28 mm ID 465.00
1702933 Large Gel Tube Assembly, 37 mm ID 480.00
1702934 Cooling Finger Assembly, includes feedline connectors 840.00
1702935 Buffer Circulation Tubing Kit, includes stopcock with tubing and connectors, 3 elution buffer circulation 89.00
lines and connectors, and electrophoresis/cooling buffer circulation lines and connectors
1702936 O-Ring Kits, 2 50.00
1702937 Dialysis Membranes, precut, 5 59.00
1702938 Frit Kit, includes support frit and elution frit 56.00
1702939 Sample Application/Overlay Buffer Kit, includes sample loading guide, syringe with tubing 86.00
1702940 Thumbscrews, 4 32.00
1702969 Lid with Power Cables 245.00
1615101 Prep Cell Starter Kit 155.00
1610323 Prep Cell Starter Kit Protein Standard, 1 ml 93.00
1702941 Elution Manifold Base 190.00
Mini Prep Cells
1702915 Mini Prep Cell with Reagent Starter Kit 2,315.00
1702908 Mini Prep Cell without Reagent Starter Kit 2,120.00
Replacement Parts and Accessories for the Mini Prep Cell
1702909 Gel Tubes, 2 95.00
1702913 Sample Application/Purge Kit 66.00
1702947 Peristaltic Pump Adaptor Kit, for 0.8 mm tubing 24.00
1702948 Elution Frit Kit, with 5 dialysis membranes, MW cutoff 3,500 64.00
1702911 Elution Frit Kit, with 5 dialysis membranes, MW cutoff 6,000 66.00
1702912 Harvest Ring Assembly, includes elution collection tubing 225.00
1702917 Mini Prep Cell Elution Chamber Top 160.00
1702918 Mini Prep Cell Casting Stand 160.00
1702916 Elution Manifold Base 110.00
8007533 Lid with Cables, for mini prep cell Inquire
216 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Protein Electrophoresis
bio-rad.com/prepelectro Preparative Electrophoresis
Ordering Information
Catalog # Description Price, U.S. $
Whole Gel Eluter Accessories
1651270 Whole Gel Eluter Template 50.00
1651275 Cellophane, 25 precut sheets 38.00
1651277 Sealing Tabs, 50 28.00
1651280 Lower Chamber Filter Paper, 21 x 21 cm, 75 precut sheets 82.00
1651281 Upper Chamber Filter Paper, 21 x 21 cm, 50 precut sheets 46.00
1702940 Thumbscrews, 4 32.00
Mini Whole Gel Eluter Accessories
1651276 Cellophane, 25 precut sheets 29.00
1651278 Sealing Tabs, 50 31.00
1651282 Lower Chamber Filter Paper, 9 x 10 cm, 50 precut sheets 37.00
1651283 Upper Chamber Filter Paper, 5 x 6 cm, 50 precut sheets 37.00
Ordering Information
Catalog # Description Price, U.S. $
1652976 Model 422 Electro-Eluter, includes electro-eluter module, membrane caps (MW cutoff 12,000–15,000), 735.00
glass tubes, frits, silicone adaptors, grommets and stoppers, buffer tank, lid with power cables
1652977* Model 422 Electro-Eluter Module, without buffer tank and lid 580.00
Accessories
1652985 Membrane Caps, clear, MW cutoff 12,000–15,000, 12 67.00
1652986 Membrane Caps, green, MW cutoff 3,500, 12 67.00
1652987 Frits, 12 46.00
1652978 Glass Tubes, 6 73.00
1652981 Silicone Adaptors, 6 64.00
1651988 Grommets and Stoppers, 8 98.00
* M odule can be used with the discontinued Mini-PROTEAN ® 3 cell. If you do not own a Mini-PROTEAN 3 cell,
order Model 422 electro-eluter #1652976.
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 217
Protein Electrophoresis
Gel Drying Equipment bio-rad.com/geldrying
218 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Protein Electrophoresis
bio-rad.com/geldrying Gel Drying Equipment
Ordering Information
Catalog # Description Price, U.S. $
1651789 HydroTech Gel Drying System, 100/120 V, includes #1651745 and #1651781 6,495.00
1651790 HydroTech Gel Drying System, 220/240 V, includes #1651746 and #1651782 6,310.00
1651745 Model 583 Gel Dryer*, 100/120 V, includes porous gel support, transparent sealing gasket, 2,465.00
filter paper backing, cellophane membrane backing, sequencing gel filter paper
1651746 Model 583 Gel Dryer*, 220/240 V, includes all items in #1651745 2,520.00
HydroTech Vacuum Pumps
1651781 HydroTech Vacuum Pump*, 100/120 V, includes pump, quick disconnect fittings for 1/4 and 4,190.00
3/8" ID vacuum tubing, vacuum tubing, drain tubing
1651782 HydroTech Vacuum Pump*, 220/240 V, includes all items in #1651781 4,140.00
HydroTech Vacuum Pump Accessories
1651783 Quick Disconnect Fitting, fits 1/4" ID tubing 49.00
1651784 Quick Disconnect Fitting, fits 3/8" ID tubing 49.00
1651785 Vacuum Tubing, 2 m, includes quick disconnect fitting, hose clamps, 2-way stopcock 125.00
1651786 Drain Tubing, 2 m, includes quick disconnect fitting, hose clamp 88.00
9100509 2-Way Stopcock 155.00
1651787 3-Way Stopcock 86.00
1651788 HydroTech Vacuum Gauge 425.00
1651791 Anti-Foam Agent, 100 ml 44.00
Double-Up Gel Drying Rack and Systems
1651796 Double-Up Gel Dryer Rack 1,140.00
1651797 Double-Up Gel Dryer System, 100/120 V, includes 2 Model 583 gel dryers (#1651745), 9,890.00
HydroTech vacuum pump (#1651781), double-up gel dryer rack (#1651796)
1651798 Double-Up Gel Dryer System, 220/240 V, includes 2 Model 583 gel dryers (#1651746), 9,890.00
HydroTech vacuum pump (#1651782), double-up gel dryer rack (#1651796)
Model 583 Drying Supports
1650962 Filter Paper Backing, for stained gels, 35 x 45 cm, 25 sheets 125.00
1650963 Cellophane Membrane Backing, clear membrane for transmission densitometry or overhead projection, 89.00
35 x 45 cm, 50 sheets
1650959 Sequencing Gel Filter Paper, for fragile sequencing gels, 35 x 45 cm, 25 sheets 125.00
1651747 Model 583 Gel Dryer Porous Gel Support*, 35 x 45 cm 180.00
1651748 Model 583 Transparent Sealing Gasket*, 41 x 51 cm 170.00
Drying Supports for Discontinued Products
1650922 Cellophane Membrane Backing, 18 x 34 cm, for Model 224, 443, and 543 slab gel dryers, 50 sheets 46.00
1650921 Thick Blot Paper, 18 x 34 cm, for Model 224, 443, and 543 slab gel dryers, 25 sheets 70.00
Gel Drying Solution
1610752 Gel Drying Solution, 1 L 46.00
* Model 583 Gel Dryer and HydroTech Vacuum Pump are not available for sale in European Union countries.
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 219
Protein Electrophoresis
Gel Drying Equipment bio-rad.com/geldrying
Specifications
GelAir Dryer GelAir Drying Frames
Timer control 0–3 hr, fully adjustable Inner dimensions 20.0 x 20.0 cm
Function modes Fan only; fan and heat; off Drying frame Molded polycarbonate bottom frame,
Dryer capacity 4 shelves, each accommodating 1 drying frame stainless-steel top frame
Dimensions (W x D x H) 27.0 x 43.0 x 30.0 cm Clamps Molded polysulfone, 8 clamps per drying frame
Weight 8 kg (18 lb) Gel capacity 4 mini (8.0 x 7.0 cm) gels, 2 Criterion™
(per frame) (13.3 x 8.7 cm) gels, 1 large (20.0 x 20.0 cm) gel
Ordering Information
Catalog # Description Price, U.S. $
1651771 elAir Drying System*, 115 V, 60 Hz, includes #1651777, 2 drying frames, 16 clamps, assembly table,
G 1,855.00
50 precut sheets of cellophane support, gel drying solution
1651772 GelAir Drying System*, 230 V, 50 Hz, includes #1651778, 2 drying frames, 16 clamps, assembly table, 1,655.00
50 precut sheets of cellophane support, gel drying solution
1651777 GelAir Dryer, 115 V, 60 Hz, gel drying oven only 1,505.00
1651778 GelAir Dryer, 230 V, 50 Hz, gel drying oven only 1,310.00
Accessories
1651775 GelAir Drying Frames, includes plastic drying frame, metal square frame, 16 clamps 210.00
1651776 GelAir Assembly Table 140.00
9207965 GelAir Plastic Drying Frame, for GelAir assembly table, does not include metal square frame Inquire
1651779 GelAir Cellophane Support, 50 precut sheets 64.00
1651780 GelAir Drying Frame Clamps, 8 66.00
1610752 Gel Drying Solution, 1 L 46.00
* GelAir Drying System is not available for sale in European Union countries.
220 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Western Blotting
bio-rad.com/blotting
Western Blotting
Bio-Rad’s western blotting products include the V3 Western Workflow™, systems for protein transfers, blotting membranes,
filter paper, premixed blotting buffers, reagents, protein standards, and detection kits.
Learn More about the Technology
Web: bio-rad.com/tech/westernblotting
1 2 3 4 5
Separate Proteins Visualize Separation Transfer Proteins Verify Transfer Validate Western Blot
Rapidly separate Immediately visualize Use the Trans-Blot Verify high-quality Perform multiplex
proteins with TGX Stain- separation using stain- Turbo system for rapid transfer by instantly imaging and validate
Free precast gels free technology and the and efficient protein imaging proteins on the results by total protein
ChemiDoc Touch imager transfer membrane normalization on the
ChemiDoc Touch imager
Ordering Information
Catalog # Description Price, U.S. $
1708381 hemiDoc Touch V3 Western Workflow for Mini Gels, includes ChemiDoc Touch imager with Image Lab
C Inquire
software, UV/stain-free sample tray, 50 Mini-PROTEAN TGX Any kD Stain-Free precast gels, SDS-PAGE
accessories, Mini-PROTEAN Tetra cell, Trans-Blot Turbo starter kit, 50 PVDF transfer packs for mini gels
1708382 ChemiDoc Touch V3 Western Workflow for Midi Gels, includes ChemiDoc Touch imager with Image Lab Inquire
software, UV/stain-free sample tray, 50 4–20% Criterion TGX Stain-Free precast gels, SDS-PAGE accessories,
Criterion cell, Trans-Blot Turbo starter kit, 50 PVDF transfer packs for midi gels
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 221
Western Blotting
bio-rad.com/blotting
Electrophoresis
1 TGX Stain-Free™ gel
Pre-transfer gel
Criterion™ cell
+ 20–30 min stain-free image to
check sample integrity
ChemiDoc™ Touch system and separation quality
Total time:
6 hr
Antibody Incubation
3 Clarity™ western
Stain-free blot image
as loading control
ECL substrate ~5 hr
2 Electrophoresis ~1 hr gel run —
3 Transfer 1–3 hr —
Total time:
16 hr
4 Antibody Incubation ~5 hr —
222 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Western Blotting
bio-rad.com/blotting Transfer Devices
Transfer Devices
Overview of Blot Transfer Systems
Rapid transfer systems — Trans-Blot® Turbo™ system (page 224) for rapid transfer of proteins, suitable for high
■■
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 223
Western Blotting
Transfer Devices bio-rad.com/blotting
■■
Efficient transfer of high- and low-MW proteins
■■
Can transfer 1–4 mini or 1–2 midi gels in a single run
■■
No cooling period required between runs
■■
pecialized protocol for Mini-PROTEAN® TGX™ gel
S
Flexible Design
transfer in 3 min
■■
Option to either use rapid preset protocols or customize
■■
No need to pre-equilibrate gels prior to transfer
transfer conditions
Ready-to-Use Transfer Packs ■■
Accommodates traditional semi-dry consumables
Ready-to-use transfer packs eliminate the need for buffer
■■
■■
Compatible with various gel types and percentages
and membrane preparation ■■
Ability to customize and store protocols within the instrument
■■
Transfer packs available with nitrocellulose and PVDF ■■
Integrated power supply means no external power supply
■■
Proprietary buffer included in each transfer pack is needed
Ready-to-Assemble (RTA) Transfer Kits Environmentally Friendly
Kits provide enough consumables for 40 blots
■■
Environmentally safe consumables eliminate disposal cost
■■
■■
onsists of pre-cut membranes, pre-cut filter pads, and
C ■■
Single-use consumables reduce waste
specially formulated transfer buffer
For More Information
■■
its available with nitrocellulose, PVDF, and low-
K Web: bio-rad.com/turbo
fluorescence PVDF Request or download bulletin: 6039
Ordering Information
Catalog # Description Price, U.S. $
1704150 Trans-Blot Turbo Transfer System, includes 2 cassettes, roller 3,005.00
1704151 Trans-Blot Turbo Cassette, 1 cassette 750.00
1704152 Trans-Blot Turbo Base, base instrument, no cassettes included 1,675.00
Mini Midi
Description (7 x 8.5 cm) Price, U.S. $ (8.5 x 13.5 cm) Price, U.S. $
Trans-Blot Turbo Transfer Pack, PVDF, pkg of 10 1704156 175.00 1704157 210.00
Trans-Blot Turbo Transfer Pack, Nitrocellulose, pkg of 10 1704158 155.00 1704159 185.00
Trans-Blot Turbo RTA Transfer Kit, Nitrocellulose, for 40 blots 1704270 285.00 1704271 370.00
Trans-Blot Turbo RTA Transfer Kit, PVDF, for 40 blots 1704272 305.00 1704273 415.00
Trans-Blot Turbo RTA Transfer Kit, LF PVDF, for 40 blots 1704274 305.00 1704275 415.00
224 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Western Blotting
bio-rad.com/blotting Transfer Devices
■■
afety cover to break the electrical current when lifted,
S
preventing electrical shock
1
In addition to western blotting, the Trans-Blot SD cell
can also transfer DNA and RNA using the unique
agarose gel semi-dry blotting support frame. The frame Expanded view of the Trans-Blot SD
2
cell assembly:
protects fragile agarose gels from compression by the 1. Safety lid. 3
electrodes. Southern and northern blot transfers can be 2. Cathode assembly with latches. 4
3. Filter paper. 5
run in 10–35 minutes. 4. Gel. 6
5. Membrane.
For More Information 6. Filter paper. 7
Web: bio-rad.com/transblotsd 7. Spring-loaded anode platform
8
Request or download bulletin: 2895 mounted on four guideposts.
8. Power cables.
9. Base.
9
Specifications
Maximum gel size (W x L) 24 x 16 cm
Buffer requirement 200 ml
Gel capacity 4 Mini-PROTEAN® precast gels, 4 Ready Gel® precast gels, 4 Mini-PROTEAN handcast gels,
3 Criterion™ gels, or 1–3 PROTEAN® II gel sandwiches*
Recommended power supply PowerPac™ HC
Dimensions (W x L x H) 37 x 24 x 11 cm
Weight 3.6 kg (7.9 lb)
* Dialysis membrane between each gel sandwich.
Ordering Information
Catalog # Description Price, U.S. $
1703940* rans-Blot SD Semi-Dry Electrophoretic Transfer Cell, includes transfer cell, agarose gel
T 1,430.00
support frame, extra thick blot paper (7 x 8.4 cm, 60 sheets; 8 x 13.5 cm, 60 sheets; 14 x 16 cm,
30 sheets; 18 x 18.5 cm, 30 sheets)
1703848 Trans-Blot SD Cell and PowerPac HC Power Supply, 100–120/220–240 V, 1,930.00
includes #1703940 and #16-5052
1703849 Trans-Blot SD Cell and PowerPac Universal Power Supply, 100–120/220–240 V, 3,800.00
includes #1703940 and #1645070
Accessories
1703947 Cathode Plate, stainless-steel upper electrode 105.00
1703942 Anode Plate, platinum-coated lower electrode 625.00
1704019 Trans-Blot SD Agarose Gel Support Frame, includes extra thick blot paper (15 x 20 cm, 30 sheets) 71.00
1703957 Trans-Blot SD DNA/RNA Blotting Kit, includes SD agarose gel support frame, extra thick blot paper 135.00
(15 x 20 cm, 30 sheets), 1 L 10x TBE buffer
* The Trans-Blot SD semi-dry transfer cell requires the use of a microprocessor-controlled power supply.
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 225
Western Blotting
Transfer Devices bio-rad.com/tankblotters
Specifications
Maximum gel size (W x L) 10 x 7.5 cm
Buffer requirement 1.2 L
Gel capacity 2 Mini-PROTEAN handcast gels, 2 Mini-PROTEAN precast gels, or 2 Ready Gel® precast gels
Recommended power supply PowerPac™ HC (PowerPac Basic is a suitable alternative)
Dimensions (W x L x H) 12 x 16 x 18 cm
Ordering Information
Catalog # Description Price, U.S. $
1703930 ini Trans-Blot Electrophoretic Transfer Cell, includes 2 gel holder cassettes, 4 foam pads,
M 600.00
modular electrode assembly, blue cooling unit, lower buffer tank, lid with cables
1703935* Mini Trans-Blot Module, without lower buffer tank and lid 485.00
1703989 Mini Trans-Blot Cell and PowerPac Basic Power Supply, includes #1703930 and #1645050 935.00
1703836 Mini Trans-Blot Cell and PowerPac HC Power Supply, includes #1703930 and #1645052 1,455.00
1658029 Mini-PROTEAN Tetra Cell and Mini Trans-Blot Module, includes 10-well, 1.0 mm, 4-gel system 1,280.00
(#1658001) and blotting module (#1703935) without lower buffer tank and lid, gel casting accessories
1658033 Mini-PROTEAN Tetra Cell, Mini Trans-Blot Module, and PowerPac Basic Power Supply, 1,515.00
includes #1658001, #1703935, and #1645050
1658034 Mini-PROTEAN Tetra Cell for Mini Precast Gels, Mini Trans-Blot Module, and PowerPac Basic 1,375.00
Power Supply, includes #1658004, #1703935, and #1645050
1658036 Mini-PROTEAN Tetra Cell for Mini Precast Gels, Mini Trans-Blot Module, and PowerPac HC 1,850.00
Power Supply, includes #1658004, #1703935, and #1645052
1658035 Mini-PROTEAN Tetra Cell, Mini Trans-Blot Module, and PowerPac HC Power Supply, 2,060.00
includes #1658001, #1703935, and #1645052
Accessories
1703931 Mini Gel Holder Cassette 96.00
1703932 Thick Blot Paper, 7.5 x 10 cm, for Mini Trans-Blot cassette, 50 sheets 48.00
1703933 Foam Pads, 8 x 11 cm, 4 39.00
1703812 Mini Trans-Blot Central Core 385.00
1703919 Blue Cooling Unit, for Mini-PROTEAN Tetra tanks 31.00
1703934 Bio-Ice Cooling Unit, for Mini-PROTEAN 3 tanks 50.00
1651279 Roller, 3.5" wide 39.00
* Also fits in the Mini-PROTEAN 3 electrophoresis cell.
226 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Western Blotting
bio-rad.com/tankblotters Transfer Devices
Specifications
Maximum gel size (W x L) 15 x 9.4 cm
Buffer requirement 1.3 L
Gel capacity 4 Mini-PROTEAN® precast gels, 4 Ready Gel® precast gels, 4 mini handcast gels, or 2 Criterion precast gels
Electrode choices Platinum-coated titanium anode and stainless-steel cathode plate electrodes*, or economical platinum wire electrodes
Recommended power supply PowerPac™ HC
Dimensions (W x L x H) 21.8 x 11.8 x 15 cm
* Plate electrodes create a high-strength electrical field with higher current densities than other electrodes, producing faster and more efficient transfers.
Ordering Information
Catalog # Description Price, U.S. $
1704070 riterion Blotter with Plate Electrodes, includes cell assembled with plate electrodes, lid with cables,
C 845.00
2 Criterion gel holder cassettes, 1 pack precut blot absorbent filter paper, 4 foam pads, gel/blot assembly
tray, roller, sealed ice block
1704071 Criterion Blotter with Wire Electrodes, includes cell assembled with wire electrodes, lid with cables, 800.00
2 Criterion gel holder cassettes, 1 pack precut blot absorbent filter paper, 4 foam pads, gel/blot assembly
tray, roller, sealed ice block
1656024 Criterion Cell/Plate Blotter System, includes #1656001 and #1704070 1,365.00
1656025 Criterion Cell/Wire Blotter System, includes #1656001 and #1704071 1,365.00
1703872 Criterion Blotter with Plate Electrodes and PowerPac HC Power Supply, includes #1704070 and #1645052 1,705.00
1703874 Criterion Blotter with Wire Electrodes and PowerPac HC Power Supply, includes#1704071 and #1645052 1,610.00
Accessories
1704076 Optional Criterion Blotter Cooling Coil 290.00
1704077 Criterion Blotter Buffer Tank 260.00
1704079 Criterion Blotter Lid 215.00
1704080 Criterion Blotter Gel Holder Cassette 110.00
1704081 Criterion Blotter Platinum Anode Plate Electrode 290.00
1704082 Criterion Blotter Stainless-Steel Cathode Plate Electrode 160.00
1704083 Criterion Blotter Wire Electrode Card, anode 185.00
1704084 Criterion Blotter Wire Electrode Card, cathode 185.00
1704085 Thick Blot Paper, 9.5 x 15.2 cm, for Criterion blotter, 50 sheets 51.00
1704086 Foam Pads, 9.5 x 15.2 cm, 4 61.00
1704087 Sealed Ice Blocks, for Criterion blotter, 2 44.00
1704089 Criterion Gel/Blot Assembly Tray 73.00
1651279 Roller, 3.5" wide 39.00
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 227
Western Blotting
Transfer Devices bio-rad.com/tankblotters
Ordering Information
Catalog # Description Price, U.S. $
1703939* rans-Blot Cell with Plate Electrodes and Super Cooling Coil, includes 2 gel holder cassettes,
T 1,185.00
buffer tank, lid with power cables, 4 foam pads, 1 pack precut blot absorbent filter paper (15 x 20 cm)
1703853* Trans-Blot Cell with Plate Electrodes, Super Cooling Coil, and PowerPac HC Power Supply, 1,920.00
includes #1703939, #1703912, and #1645052
1703946 Trans-Blot Cell with Plate Electrodes, includes 2 gel holder cassettes, buffer tank, lid with power 1,225.00
cables, 4 foam pads, 1 pack precut blot absorbent filter paper (15 x 20 cm)
1703850 Trans-Blot Cell with Plate Electrodes and PowerPac HC Power Supply, includes #1703946 and #1645052 1,785.00
1703910 Trans-Blot Cell with Wire Electrodes, includes 2 gel holder cassettes, buffer tank, lid with power cables, 880.00
4 foam pads, 1 pack precut blot absorbent filter paper (15 x 20 cm)
1703825 Trans-Blot Cell with Wire Electrodes and PowerPac HC Power Supply, includes #1703910 and #1645052 1,655.00
Accessories
1703914 Foam Pads, 15.5 x 20.5 cm, 6 50.00
1703956 Thick Blot Paper, 15 x 20 cm, for Trans-Blot cassette, 25 sheets 48.00
1703960 Extra Thick Blot Paper, 15 x 20 cm, 30 sheets 61.00
1703943 Trans-Blot Platinum Anode Plate Electrode 570.00
1703944 Trans-Blot Stainless-Steel Cathode Plate Electrode 250.00
1703945 Trans-Blot Plate Electrode Pair, platinum anode and stainless-steel cathode 740.00
1703920 Trans-Blot Standard Wire Electrode Card, cathode 195.00
1703921 Trans-Blot Standard Wire Electrode Card, anode 235.00
1703912 Super Cooling Coil, required for all high-intensity transfers 195.00
1703913 Gel Holder Cassette, includes 2 foam pads 100.00
1703922 Trans-Blot Cell Buffer Tank 255.00
1703923 Trans-Blot Cell Lid with Power Cables 235.00
* Trans-Blot cells require the super cooling coil for high-intensity transfers; the super cooling coil is also recommended for all applications
using plate electrodes.
228 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Western Blotting
bio-rad.com/tankblotters Transfer Devices
Specifications
Maximum gel size (W x L) 26.5 x 28 cm
Buffer requirement 10–12 L
Gel capacity 27 Mini-PROTEAN® precast gels, 27 Mini-PROTEAN handcast gels, 27 Ready Gel® precast gels,
12 Criterion™ gels, or 3 PROTEAN® II XL gels
Recommended power supply PowerPac™ HC
Dimensions (W x L x H) 30 x 17.3 x 39.4 cm
Ordering Information
Catalog # Description Price, U.S. $
1703990* rans-Blot Plus Cell with Plate Electrodes and Super Cooling Coil, includes 3 gel holder cassettes, buffer tank,
T 3,115.00
lid with power cables, 6 foam pads, 1 pack blot absorbent filter paper (26.5 x 28 cm, 30 sheets), roller, stirbar
1703991 Trans-Blot Plus Cell and PowerPac HC Power Supply, 100–120/220–240 V, 3,845.00
includes #1703990 and #1645052
1703992 Trans-Blot Plus Cell and PowerPac Universal Power Supply, 100–120/220–240 V, 5,465.00
includes #1703990 and #1645070
1654144 PROTEAN Plus Dodeca Cell (100/120 V), Trans-Blot Plus Cell, and PowerPac Universal Power Supply, Inquire
includes #1654150, #1703990, and #1645070
1654145 PROTEAN Plus Dodeca Cell (220/240 V), Trans-Blot Plus Cell, and PowerPac Universal Power Supply, Inquire
includes #1654151, #1703990, and #1645070
Accessories
1703995 Foam Pads, 27 x 28.5 cm, 2 51.00
1703997 Stirbar 25.00
1703998 Trans-Blot Plus Roller, 6 in wide 28.00
1704990 Trans-Blot Plus Super Cooling Coil 330.00
1704991 Trans-Blot Plus Platinum Anode Plate Electrode 760.00
1704992 Trans-Blot Plus Stainless-Steel Cathode Plate Electrode 370.00
* Trans-Blot cells require the super cooling coil for high-intensity transfers; the super cooling coil is also recommended
for all applications using plate electrodes.
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 229
Western Blotting
Transfer Devices bio-rad.com/microfiltration
■■
Resistance to 100% ethanol, strong acid, and NaOH
■■
Autoclavability Slot format allows easy
densitometric analyses to
■■
Sealing gasket to eliminate lateral leakage determine relative
amounts of protein
■■
Easy sample application with microplate-based spacing
■■
Flow valve (three-way) for adjustable vacuum
Three-way vacuum valve allows sample
loading by gravity and quick washes
Specifications
Bio-Dot Apparatus Bio-Dot SF Apparatus
Format Dot blot Slot blot
Samples 96-well, 8 x 12 format 48-well, 6 x 8 format
Sample volume 50–600 µl 50–500 µl
Well size 3 mm diameter 7 x 0.75 mm
Quantitation with Yes, but Bio-Dot SF Yes
densitometer unit recommended
Overnight incubations Yes No
Membrane size (W x L) 12 x 9 cm 12 x 9 cm
Dimensions (W x H x D) 9 x 6.5 x 12" 9.5 x 7 x 12"
Weight 1.1 kg (2.5 lb) 1.2 kg (2.6 lb)
Ordering Information
Catalog # Description Price, U.S. $
1703938 io-Dot Microfiltration System, includes Bio-Dot apparatus (#1706545) and Bio-Dot SF module (#1706543)
B 1,055.00
templates, vacuum manifold base, gasket support plates, gasket
1706545 Bio-Dot Apparatus, includes Bio-Dot sample template, vacuum manifold base, gasket support plate, gasket 665.00
1706547 Bio-Dot Module, without vacuum manifold base, for conversion of Bio-Dot SF to Bio-Dot apparatus 505.00
1706542 Bio-Dot SF Apparatus, includes Bio-Dot SF sample template, vacuum manifold base, 665.00
gasket support plate, gasket, filter paper
1706543 Bio-Dot SF Module, without vacuum manifold base, for conversion of Bio-Dot to Bio-Dot SF apparatus 490.00
Accessories
1706546 Bio-Dot Gaskets, 3 86.00
1706544 Bio-Dot SF Gaskets, 2 65.00
1620161 Bio-Dot/Bio-Dot SF Filter Paper, 11.3 x 7.7 cm, 60 sheets 43.00
230 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Western Blotting
bio-rad.com/microfiltration Transfer Devices
Ordering Information
Catalog # Description Price, U.S. $
1704017 Mini-PROTEAN II Multiscreen Apparatus, includes 2 sample templates, 2 gaskets, base plate 785.00
1704018 Multiscreen Gaskets, 2 80.00
Ordering Information
Catalog # Description Price, U.S. $
1703902 Mini Incubation Trays, 20 120.00
1703903 Mini Incubation Trays, 100 565.00
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 231
Western Blotting
Membranes and Filter Papers bio-rad.com/blottingmembranes
232 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Western Blotting
bio-rad.com/blottingmembranes Membranes and Filter Papers
Ordering Information
Catalog # Description Recommended Uses Price, U.S. $
Nitrocellulose Membranes (0.2 µm)
1620112 Nitrocellulose Membrane, 0.2 µm, 30 cm x 3.5 m, 1 roll Transfer of low MW 345.00
1620212 Nitrocellulose/Filter Paper Sandwiches, 0.2 µm, 7 x 8.4 cm, 20 pack proteins or nucleic acids 150.00
1620213 Nitrocellulose/Filter Paper Sandwiches, 0.2 µm, 7 x 8.4 cm, 50 pack (has smaller pore size) 325.00
1620232 Nitrocellulose/Filter Paper Sandwiches, 0.2 µm, 8.5 x 13.5 cm, 20 pack 195.00
1620233 Nitrocellulose/Filter Paper Sandwiches, 0.2 µm, 8.5 x 13.5 cm, 50 pack 480.00
1620146 Nitrocellulose Membranes, 0.2 µm, 7 x 8.4 cm, 10 sheets 69.00
1620168 Nitrocellulose Membranes, 0.2 µm, 8.5 x 13.5 cm, 10 sheets 97.00
1620147 Nitrocellulose Membranes, 0.2 µm, 13.5 x 16.5 cm, 10 sheets 200.00
1620150 Nitrocellulose Membranes, 0.2 µm, 20 x 20 cm, 5 sheets 170.00
1620252 Nitrocellulose Membranes, 0.2 µm, 26.5 x 28 cm, 10 sheets 235.00
Nitrocellulose Membranes (0.45 µm)
1620115 Nitrocellulose Membrane, 0.45 µm, 30 cm x 3.5 m, 1 roll Transfer of low MW antigens, 275.00
1620214 Nitrocellulose/Filter Paper Sandwiches, 0.45 µm, 7 x 8.4 cm, 20 pack immunoglobulins, glycoprotein 140.00
1620215 Nitrocellulose/Filter Paper Sandwiches, 0.45 µm, 7 x 8.4 cm, 50 pack receptor proteins, histones 305.00
1620234 Nitrocellulose/Filter Paper Sandwiches, 0.45 µm, 8.5 x 13.5 cm, 20 pack and nonhistones, etc.; capillary 215.00
1620235 Nitrocellulose/Filter Paper Sandwiches, 0.45 µm, 8.5 x 13.5 cm, 50 pack Southern blotting of ssDNA and 480.00
RNA <500 bp (use Zeta-Probe
1620145 Nitrocellulose Membranes, 0.45 µm, 7 x 8.4 cm, 10 sheets 62.00
membranes for blotting ssDNA
1620167 Nitrocellulose Membranes, 0.45 µm, 8.5 x 13.5 cm, 10 sheets 96.00
and RNA of all sizes)
1620117 Nitrocellulose Membranes, 0.45 µm, 9 x 12 cm, 10 sheets 96.00
1620148 Nitrocellulose Membranes, 0.45 µm, 11.5 x 16 cm, 10 sheets 125.00
1620114 Nitrocellulose Membranes, 0.45 µm, 15 x 9.2 cm, 10 sheets 98.00
1620116 Nitrocellulose Membranes, 0.45 µm, 15 x 15 cm, 10 sheets 135.00
1620113 Nitrocellulose Membranes, 0.45 µm, 20 x 20 cm, 5 sheets 120.00
1620251 Nitrocellulose Membranes, 0.45 µm, 26.5 x 28 cm, 10 sheets 160.00
Supported Nitrocellulose Membranes (0.2 µm)
1620097 Supported Nitrocellulose Membrane, 0.2 µm, 30 cm x 3 m, 1 roll Protein and nucleic acid blotting 340.00
1620095 Supported Nitrocellulose Membranes, 0.2 µm, 7 x 8.4 cm, 10 sheets 62.00
1620071 Supported Nitrocellulose Membranes, 0.2 µm, 8.5 x 13.5 cm, 10 sheets 95.00
Supported Nitrocellulose Membranes (0.45 µm)
1620094 Supported Nitrocellulose Membrane, 0.45 µm, 30 cm x 3 m, 1 roll Protein and nucleic acid blotting 325.00
1620090 Supported Nitrocellulose Membranes, 0.45 µm, 7 x 8.4 cm, 10 sheets 62.00
1620070 Supported Nitrocellulose Membranes, 0.45 µm, 8.5 x 13.5 cm, 10 sheets 96.00
PVDF Membranes
The chemically resistant PVDF membrane has very high Immun-Blot Low Fluorescence PVDF Membrane
protein binding capacity and resistance to tearing and Optimized for fluorescence applications, the low
cracking, even after repeated stripping and reprobing. fluorescence property of the membrane enhances
All Bio-Rad PVDF membranes have a 0.2 µm pore size. image quality and improves sensitivity of all fluorescence
detection protocols. It is ideal for multiplex, fluorescence
Immun-Blot® PVDF for Western Blotting
western blotting, and chemifluorescence applications.
This membrane is ideal for chemiluminescent and
The membrane is also compatible with other detection
colorimetric western blots because it retains target protein
methods such as chemiluminescence and colorimetric
very strongly but reduces nonspecific protein binding that
detection. This membrane is highly recommended for the
can obscure high-sensitivity detection. Binding capacity is
V3 Western Workflow™.
150–160 µg/cm2.
For More Information
Sequi-Blot™ PVDF for Protein Sequencing Web: bio-rad.com/v3
This membrane gives outstanding performance in Request or download bulletins: 2212 and 6116
protein sequencing, even for low-abundance samples.
Sequi-Blot PVDF retains all transferred protein and has a
binding capacity of 170–200 µg/cm2.
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 233
Western Blotting
Membranes and Filter Papers bio-rad.com/blottingmembranes
Ordering Information
Catalog # Description Price, U.S. $
Immun-Blot PVDF Membranes
1620177 Immun-Blot PVDF Membrane, 26 cm x 3.3 m, 1 roll 325.00
1620218 Immun-Blot PVDF/Filter Paper Sandwiches, 7 x 8.4 cm, 20 pack 215.00
1620219 Immun-Blot PVDF/Filter Paper Sandwiches, 7 x 8.4 cm, 50 pack 490.00
1620238 Immun-Blot PVDF/Filter Paper Sandwiches, 8.5 x 13.5 cm, 20 pack 330.00
1620239 Immun-Blot PVDF/Filter Paper Sandwiches, 8.5 x 13.5 cm, 50 pack 665.00
1620174 Immun-Blot PVDF Membranes, 7 x 8.4 cm, 10 sheets 86.00
1620175 Immun-Blot PVDF Membranes, 10 x 15 cm, 10 sheets 120.00
1620176 Immun-Blot PVDF Membranes, 20 x 20 cm, 10 sheets 250.00
1620255 Immun-Blot PVDF Membranes, 25 x 28 cm, 10 sheets 245.00
Sequi-Blot PVDF Membranes
1620184 Sequi-Blot PVDF Membrane, 26 cm x 3.3 m, 1 roll 370.00
1620237 Sequi-Blot PVDF/Filter Paper Sandwiches, 8.5 x 13.5 cm, 50 pack 790.00
1620186 Sequi-Blot PVDF Membranes, 7 x 8.4 cm, 10 sheets 87.00
1620180 Sequi-Blot PVDF Membranes, 10 x 15 cm, 10 sheets 135.00
1620181 Sequi-Blot PVDF Membranes, 15 x 15 cm, 10 sheets 155.00
1620182 Sequi-Blot PVDF Membranes, 20 x 20 cm, 10 sheets 255.00
Immun-Blot Low-Fluorescence PVDF Membranes
1620260 Low Fluorescence PVDF/Filter Paper Sandwiches, 7 x 8.5 cm, 10 pack 110.00
1620261 Low Fluorescence PVDF/Filter Paper Sandwiches, 7 x 8.5 cm, 20 pack 215.00
1620262 Low Fluorescence PVDF/Filter Paper Sandwiches, 8.5 x 13.5 cm, 10 pack 170.00
1620263 Low Fluorescence PVDF/Filter Paper Sandwiches, 8.5 x 13.5 cm, 20 pack 330.00
1620264 Low Fluorescence PVDF Roll, 28 x 3.8 m, 1 roll 645.00
Ordering Information
Description Zeta-Probe Price, U.S. $ Zeta-Probe GT Price, U.S. $
Zeta-Probe and Zeta-Probe GT Membranes
30 cm x 3.3 m, 1 roll 1620159 410.00 1620196 485.00
20 cm x 3.3 m, 1 roll 1620165 305.00 1620197 390.00
7 x 10 cm, 15 sheets 1620206 150.00 1620208 185.00
9 x 12 cm, 15 sheets 1620153 165.00 1620190 190.00
continues
234 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Western Blotting
bio-rad.com/blottingmembranes Membranes and Filter Papers
Ordering Information
Description Zeta-Probe Price, U.S. $ Zeta-Probe GT Price, U.S. $
Zeta-Probe and Zeta-Probe GT Membranes (cont.)
10 x 15 cm, 15 sheets 1620154 190.00 1620191 215.00
15 x 15 cm, 15 sheets 1620155 215.00 1620192 305.00
15 x 20 cm, 15 sheets 1620156 285.00 1620193 375.00
20 x 20 cm, 15 sheets 1620157 375.00 1620194 470.00
20 x 25 cm, 3 sheets 1620158 120.00 1620195 150.00
Filter Paper
Bio-Rad offers a range of filter papers for blotting applications, including filter paper precut to fit standard gel sizes.
Ordering Information
Catalog # Description Recommended Uses Price, U.S. $
Blot Absorbent Filter Paper (Extra Thick)
1703965 Extra Thick Blot Paper, 7.5 x 10 cm, for Ready Gel or All blotting applications using the 56.00
Mini-PROTEAN Tetra gels, 60 sheets Trans-Blot SD cell or Trans-Blot
1703966 Extra Thick Blot Paper, 7 x 8.4 cm, for Ready Gel or cell (precut to gel dimensions from 56.00
Mini-PROTEAN Tetra gels, 60 sheets well to bottom of gel)
1703967 Extra Thick Blot Paper, 8 x 13.5 cm, for Criterion precast gels, 60 sheets 56.00
1703968 Extra Thick Blot Paper, 14 x 16 cm, for PROTEAN II xi gels, 30 sheets 59.00
1703969 Extra Thick Blot Paper, 19 x 18.5 cm, for PROTEAN II XL gels, 30 sheets 61.00
1703958 Extra Thick Blot Paper, 10 x 15 cm, 30 sheets 56.00
1703959 Extra Thick Blot Paper, 15 x 15 cm, 30 sheets 59.00
1703960 Extra Thick Blot Paper, 15 x 20 cm, 30 sheets 61.00
Blot Absorbent Filter Paper (Thick)
1703932 Thick Blot Paper, 7.5 x 10 cm, for Mini Trans-Blot cassette, 50 sheets All blotting applications requiring 48.00
1704085 Thick Blot Paper, 9.5 x 15.2 cm, for Criterion blotter, 50 sheets thick, high wet strength filter paper 51.00
1703955 Thick Blot Paper, 14 x 16 cm, for PROTEAN II xi gels, 25 sheets 50.00
1703956 Thick Blot Paper, 15 x 20 cm, for Trans-Blot cassette, 25 sheet 48.00
1650921 Thick Blot Paper, 18 x 34 cm, for Model 224, 443, 70.00
and 543 slab gel dryers, 25 sheets
1620161 Bio-Dot/Bio-Dot SF Filter Paper, 7.7 x 11.3 cm, 60 sheets 43.00
1650962 Filter Paper Backing, 35 x 45 cm, for stained gels, 25 sheets 125.00
Blot Absorbent Filter Paper (Thin)
1620118 Thin Blot Paper, 33 cm x 3 m, 1 roll All blotting applications requiring 43.00
thin, high wet strength filter paper
Bio-Rad offers a selection of stains for blotting applications; see page 243. Tracking dyes can be found on page 259.
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 235
Western Blotting
Blotting Buffers and Reagents bio-rad.com/blotting
Two transfer buffers are available: 10x Tris/glycine and 10x Tris/CAPS. Premixed blocking buffers, available as 1x PBS
with casein and 1x TBS with casein, take the time and effort out of solubilizing casein. Bio-Rad offers a complete line of
reagents for preparation of buffers to your own specifications.
Ordering Information
Catalog # Description Price, U.S. $ Catalog # Description Price, U.S. $
Blot Transfer and Processing Buffers Detergents and Blocking Buffers
1610734 10x Tris/Glycine, 1 L 28.00 1706537 Gelatin, EIA grade, 200 g 56.00
1610771 10x Tris/Glycine, 5 L cube 94.00 1706404 Blotting-Grade Blocker, 47.00
1610778 10x Tris/CAPS, 1L 74.00 nonfat dry milk, 300 g
1610780 10x Phosphate Buffered Saline, 1 L 44.00 1706531 Tween 20, EIA grade, 100 ml 53.00
1706435 10x Tris Buffered Saline, 1 L 40.00 1610781 10% (w/v) Tween 20, for easy 36.00
pipetting, 1 L
1610418 SDS Solution, 20% (w/v), 1 L 120.00
1610783 1x Phosphate Buffered Saline 94.00
with 1% Casein*, 1 L
1610782 1x Tris Buffered Saline with 1% 94.00
Casein*, 1 L
Reagents
1610610 Dithiothreitol (DTT)**, 1 g 27.00 1610710 2-Mercaptoethanol, 25 ml 23.00
1610611 Dithiothreitol (DTT)**, 5 g 81.00 1632101 Tributylphosphine (TBP), 44.00
1610729 EDTA, 500 g 72.00 200 mM, 0.6 ml
1706537 Gelatin, EIA grade, 200 g 56.00 1610713 Tricine, 500 g 210.00
1610717 Glycine, 250 g 24.00 1610716 Tris, 500 g 33.00
1610718 Glycine, 1 kg 53.00 1610719 Tris, 1 kg 84.00
1610730 Urea, 250 g 27.00
* Store at 2–8°C.
** Store desiccated at 2–8°C; store other reagents at room temperature, dry, and away from direct sunlight.
Hazardous shipping charges may apply.
236 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Western Blotting
bio-rad.com/blotdetection Immunodetection Reagents and Kits
Key
HRP Horseradish peroxidase
AP Alkaline phosphatase
TransferAesolved Pr
Onto Membrane
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 237
Western Blotting
Immunodetection Reagents and Kits bio-rad.com/blotdetection
Chemiluminescence Detection
Chemiluminescent western blot detection offers highly sensitive detection of proteins bound to blotting membranes.
Most specific antigen detection methods are based on HRP (horseradish peroxidase) or AP (alkaline phosphatase)
secondary antibody conjugates. The signal can be captured with film or dedicated imaging equipment
(see page 280).
Ordering Information
Catalog # Description Price, U.S. $
1705060 larity Western ECL Substrate, 200 ml size contains Clarity western peroxide reagent,
C 135.00
100 ml, and Clarity western luminol/enhancer reagent, 100 ml
1705061 Clarity Western ECL Substrate, 500 ml size contains Clarity western peroxide reagent, 255.00
250 ml, and Clarity western luminol/enhancer reagent, 250 ml
238 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Western Blotting
bio-rad.com/blotdetection Immunodetection Reagents and Kits
Ordering Information
Catalog # Description Substrate Enhancer* Antibody Price, U.S. $
Immun-Star AP Kits** and Components
1705010 GAM-AP Detection Kit • • • 315.00
1705011 GAR-AP Detection Kit • • • 315.00
1705018 AP Substrate, 125 ml • 260.00
1705012 AP Substrate Pack • • 290.00
* The enhancer is used on nitrocellulose blots but is not optimized for PVDF blots. Additional testing is recommended to
determine appropriate conditions for PVDF blots.
** GAM, goat anti-mouse; GAR, goat anti-rabbit.
Colorimetric Detection
Enzymes such as HRP or AP convert several substrates to a colored precipitate. As the precipitate accumulates on the
blot, a colored signal develops. The reaction can be monitored and stopped when the desired signal over background
is observed. Colorimetric detection is easier to perform than film-based chemiluminescence detection; however,
the method’s single end-point result does not allow multiple exposures of chemiluminescent methods. Colorimetric
detection is typically considered a medium-sensitivity method compared to radioactive or chemiluminescence detection.
However, Bio-Rad offers amplified colorimetric systems that provide high sensitivity comparable to or exceeding that of
chemiluminescent detection.
For More Information
Web: bio-rad.com/blotdetection
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 239
Western Blotting
Immunodetection Reagents and Kits bio-rad.com/blotdetection
Ordering Information
Catalog # Description Price, U.S. $
Opti-4CN Kits*
1708235 Opti-4CN Substrate Kit 175.00
1708236 Opti-4CN Goat Anti-Rabbit Detection Kit 190.00
1708237 Opti-4CN Goat Anti-Mouse Detection Kit 190.00
Amplified Opti-4CN Kits*
1708238 Amplified Opti-4CN Substrate Kit 420.00
1708240 Amplified Opti-4CN Goat Anti-Mouse Detection Kit 480.00
1708239 Amplified Opti-4CN Goat Anti-Rabbit Detection Kit 470.00
Immun-Blot HRP Assay Kits, with 4CN**
1706463 Goat Anti-Rabbit IgG (H + L)-HRP Assay Kit 250.00
1706464 Goat Anti-Mouse IgG (H + L)-HRP Assay Kit 255.00
1706465 Goat Anti-Human IgG (H + L)-HRP Assay Kit 250.00
Premixed Substrate Reagents
1706431 HRP Conjugate Substrate Kit, contains premixed 4CN, hydrogen peroxide solutions, 110.00
color development buffer; makes 1 L color development solution
Individual Blotting Substrates
1706534 HRP Color Development Reagent, 4CN, 5 g 56.00
1706535 HRP Color Development Reagent, DAB, 5 g 145.00
* Each kit contains enough reagent for 2,500 cm2 of membrane or approximately 50 mini blots.
** K its contain 0.5 ml of specific HRP blotting-grade conjugate; each kit provides reagents (blotting-grade TBS buffer,
Tween 20 detergent, gelatin blocking reagent, and 4CN substrate solution) for 200 assays on a 0.6–0.8 x 9.2 cm
nitrocellulose strip using a total volume of 5.0 ml.
240 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Western Blotting
bio-rad.com/blotdetection Immunodetection Reagents and Kits
Colorimetric AP Detection
Ordering Information
Catalog # Description Price, U.S. $
Opti-4CN Kits*
1706460 Goat Anti-Rabbit IgG (H + L)-AP Assay Kit 295.00
1706461 Goat Anti-Mouse IgG (H + L)-AP Assay Kit 295.00
1706462 Goat Anti-Human IgG (H + L)-AP Assay Kit 295.00
Premixed Substrate Reagents
1706432 AP Conjugate Substrate Kit, contains premixed BCIP and NBT solutions and color development buffer; 155.00
makes 1 L color development solution
* K its contain 0.5 ml of specific AP blotting-grade conjugate; each kit provides reagents (blotting grade TBS buffer,
Tween 20 detergent, gelatin blocking reagent, and BCIP and NBT substrate solution) for 200 assays on a 0.6–0.8 x 9.2 cm
nitrocellulose strip using a total volume of 5.0 ml.
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 241
Western Blotting
Immunodetection Reagents and Kits bio-rad.com/blotdetection
Blotting-Grade Reagents
Detergents and blocking reagents for western blotting are
available individually.
Conjugate Specifications*
Products Volume, ml Recommended Dilution Products Volume, ml Recommended Dilution
Immun-Star™ GAR 2 1:10,000 Goat anti-rabbit lgG-AP 1 1:3,000
Immun-Star GAM 2 1:10,000 Goat anti-mouse lgG-AP 1 1:3,000
Avidin-HRP 2 1:1,000–1:3,000 Goat anti-human lgG-AP 1 1:3,000
Goat anti-rabbit (H + L) 2 1:3,000 Avidin-AP 1 1:1,000–1:3,000
Goat anti-mouse (H + L) 2 1:3,000 Biotinylated-AP 1 1:3,000
Goat anti-human (H + L) 2 1:3,000 Biotinylated-GAR (H + L), 1 1:3,000
Protein A-HRP 1 1:3,000 human lgG adsorbed
Protein G-HRP 1 1:3,000
* Shelf life of conjugates is one year when stored at 4°C.
Ordering Information
Catalog # Description Price, U.S. $ Catalog # Description Price, U.S. $
Blotting-Grade Conjugates, HRP
1705046 Immun-Star Goat Anti-Rabbit 210.00 1706522 Protein A-HRP, 1 ml 170.00
(GAR)-HRP, lyophilized 1706425 Protein G-HRP, 1 ml 150.00
1705047 Immun-Star Goat Anti-Mouse (GAM)- 210.00 1706528 Avidin-HRP, 2 ml 115.00
HRP, lyophilized
1706515 Goat Anti-Rabbit IgG (H + L)-HRP, 2 ml 185.00
1706516 Goat Anti-Mouse IgG (H + L)-HRP, 2 ml 185.00
1721050 Goat Anti-Human IgG (H + L)-HRP, 2 ml 160.00
Blotting-Grade Conjugates, AP
1706518 Goat Anti-Rabbit IgG-AP, 1 ml 190.00 1706521 Goat Anti-Human IgG-AP, 1 ml 185.00
1706520 Goat Anti-Mouse IgG-AP, 1 ml 190.00 1706403 Biotinylated AP, 1 ml 170.00
Detergents and Blocking Reagents
1706537 Gelatin, EIA grade, 200 g 56.00 1610418 SDS Solution, 20% (w/v), 1 L 120.00
1706404 Blotting-Grade Blocker, nonfat dry milk, 47.00 1610783 1x Phosphate Buffered Saline 94.00
300 g with 1% Casein,* 1 L
1706531 Tween 20, EIA grade, 100 ml 53.00 1610782 1x Tris Buffered Saline with 94.00
1610781 10% Tween 20, for easy pipetting, 1 L 36.00 1% Casein,* 1 L
* Store at 2–8°C.
242 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Western Blotting
bio-rad.com/blotdetection Immunodetection Reagents and Kits
Ordering Information
Catalog # Description Price, U.S. $
1703127 SYPRO Ruby Protein Blot Stain, 200 ml 175.00
1610400 Coomassie Brilliant Blue R-250, 10 g 20.00
1706527 Colloidal Gold Total Protein Stain, 500 ml 150.00
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 243
Nucleic Acid Electrophoresis and Blotting
DNA Electrophoresis Systems bio-rad.com/DNAelectro
■■
Combs to fit virtually every need Components of the Mini-Sub cell GT cell.
■■
Clear plastic construction for easy sample visualization
■■
uffer recirculation ports for applications that require
B
high voltages or extended runs
■■
Easy-to-replace electrode cassettes
■■
IEC 1010 (EN 61010) electrical safety certification
For More Information
Web: bio-rad.com/DNAelectro
Request or download bulletin: 2660
244 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Nucleic Acid Electrophoresis and Blotting
bio-rad.com/DNAelectro DNA Electrophoresis Systems
* The mini ReadySub-Cell™ GT cell is a Mini-Sub cell GT cell dedicated to running ReadyAgarose precast gels, gel size 7 x 10 cm;
sample throughput is 8-, 12-, or 2 x 8-well. This cell does not include casting gates, tray, or combs.
** The wide mini ReadySub-Cell GT cell is a wide Mini-Sub cell GT cell dedicated to running ReadyAgarose precast gels, gel size 15 x 10 cm;
sample throughput is 20-, 32-, 2 x 32-, or 4 x 26-well. This cell does not include casting gates, tray, or combs.
*** Sample throughput value assumes 1–2 combs per gel.
†
Sample throughput value assumes 1–4 combs per gel.
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 245
Nucleic Acid Electrophoresis and Blotting
DNA Electrophoresis Systems bio-rad.com/DNAelectro
Ordering Information
UVTP Tray, cm Combs
Casting Gel PowerPac™ Basic Power
Catalog # Gates Caster 7 x 7* 7 x 10 8-Well 15-Well Supply (#16-5050) Price, U.S. $
Mini-Sub Cell GT Systems**
1704406 • • • • 380.00
1704466 • • • 375.00
1704486 • • • • • 455.00
1704467 • • • • 455.00
1640300 • • • • • 840.00
1704487 310.00
1640303 • 690.00
246 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Nucleic Acid Electrophoresis and Blotting
bio-rad.com/DNAelectro DNA Electrophoresis Systems
Ordering Information
UVTP Tray, cm Combs
Casting Gel PowerPac™ Basic Power
Catalog # Gates Caster 15 x 7* 15 x 10 15-Well 20-Well Supply (#1645050) Price, U.S. $
Wide Mini-Sub Cell GT Systems**
1704405 • • • • 505.00
1704468 • • • 525.00
1704485 • • • • • 575.00
1704469 • • • • 575.00
1640301 • • • • • 965.00
1704489 455.00
1640304 • 840.00
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 247
Nucleic Acid Electrophoresis and Blotting
DNA Electrophoresis Systems bio-rad.com/DNAelectro
Sub-Cell® GT Cell
Ordering Information
UVTP Tray, cm Combs PowerPac™ Basic
Casting Gel Power Supply
Catalog # Gates Caster 15 x 10 15 x 15* 15 x 20 15 x 25 15-Well 20-Well (#1645050) Price, U.S. $
Sub Cell GT Systems**
1704401 • • • 670.00
1704402 • • • • 670.00
1704403 • • • 685.00
1704404 • • • 670.00
1704481 • • • • 735.00
1704482 • • • • • 735.00
1704483 • • • • 735.00
1704484 • • • • 735.00
1640302 • • • • • • 1,130.00
248 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Nucleic Acid Electrophoresis and Blotting
bio-rad.com/DNAelectro DNA Electrophoresis Systems
Ordering Information
Width of Length of Volume, µl
Catalog # # of Wells Height Thickness, mm Well, mm Teeth, mm (in 5 mm deep gel) Price, U.S. $
Combs for Wide Mini Sub-Cell and Sub-Cell GT Systems*
1704449 30 Fixed 1.5 2.7 14.0 20.2 57.00
1704344 30 Adjustable 1.5 2.7 19.1 20.2 57.00
1704447 20 Fixed 0.75 4.8 14.0 18.2 57.00
1704448** 20 Fixed 1.5 4.8 14.0 36.3 57.00
1704321 20 Adjustable 0.75 4.8 19.1 18.2 57.00
1704322 20 Adjustable 1.5 4.8 19.1 36.4 57.00
1704445 15 Fixed 0.75 5.5 14.0 20.7 57.00
1704446** 15 Fixed 1.5 5.5 14.0 41.4 57.00
1704323 15 Adjustable 0.75 5.5 19.1 20.7 57.00
1704324 15 Adjustable 1.5 5.5 19.1 41.4 57.00
1704443 10 Fixed 0.75 9.9 14.0 37.0 57.00
1704444 10 Fixed 1.5 9.9 14.0 74.0 57.00
1704325 10 Adjustable 0.75 9.9 19.1 37.0 57.00
1704326 10 Adjustable 1.5 9.9 19.1 74.0 57.00
Preparative Combs for Sub-Cell GT Systems*
4 preparative
1704442 Fixed 1.5 26.4 14.0 200.0 57.00
2 reference
2 preparative
1704441 Adjustable 1.5 50.3 14.0 377.0 57.00
2 reference
1 preparative
1704440 Fixed 1.5 106.4 14.0 800.0 57.00
2 reference
1 preparative
1704328 Adjustable 3.0 106.4 14.0 1,596.0 57.00
2 reference
Multichannel Pipet–Compatible Combs for Wide Mini Sub-Cell and Sub-Cell GT Systems*
1704456 26 Fixed 0.75 2.9 14.0 10.9 57.00
1704457 26 Fixed 1.5 2.9 14.0 21.8 57.00
1704454 18 Fixed 0.75 2.9 14.0 11.2 57.00
1704455 18 Fixed 1.5 2.9 14.0 22.5 57.00
1704452 14 Fixed 0.75 5.8 14.0 22.5 57.00
1704453 14 Fixed 1.5 5.8 14.0 45.0 57.00
1704450 10 Fixed 0.75 5.8 14.0 22.5 57.00
1704451 10 Fixed 1.5 5.8 14.0 45.0 57.00
* Fixed-height combs must be used with GT gel trays. Adjustable-height combs require comb holder, #1704320.
** Combs included in systems.
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 249
Nucleic Acid Electrophoresis and Blotting
DNA Electrophoresis Systems bio-rad.com/DNAelectro
Ordering Information
UVTP Tray, cm Combs
Casting Gel PowerPac™ Basic Power
Catalog # Gates Caster 25 x 10* 25 x 15 26-Well 51-Well Supply (#1645050) Price, U.S. $
Sub-Cell Model 96 Systems
1704502 • • • • 745.00
1704503 • • • 745.00
1704500 • • • • • 1,065.00
1704501 • • • • 1,065.00
1640305 • • • • • • 1,470.00
250 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Nucleic Acid Electrophoresis and Blotting
bio-rad.com/DNAelectro DNA Electrophoresis Systems
Ordering Information
UVTP Tray, cm Combs PowerPac™ Basic
Casting Gel Power Supply
Catalog # Gates Caster 25 x 10 25 x 15* 25 x 20 25 x 25 26-Well 51-Well (#1645050) Price, U.S. $
Sub-Cell Model 192 Systems
1704508 • • • 800.00
1704509 • • • • 800.00
1704510 • • • 800.00
1704511 • • • 785.00
1704504 • • • • 955.00
1704505 • • • • • 1,180.00
1704506 • • • • 1,205.00
1704507 • • • • 955.00
1640306 • • • • • • 1,590.00
* Allows casting gels in the cell using casting gates; other gel sizes can be cast with a gel caster.
** Combs for Sub-Cell Model 192 cells can be used with Sub-Cell Model 96 cells and vice versa. Adjustable-height combs
require comb holder, #1704525. Each system includes one comb holder.
*** Multichannel pipet compatible.
† Combs included in systems.
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 251
Nucleic Acid Electrophoresis and Blotting
DNA Electrophoresis Systems bio-rad.com/agarosegel
Ordering Information
Description 8-Well 2 x 8-Well 2 x 8-Well Price, U.S. $
Mini ReadyAgarose Gels, TBE
1.0% plus ethidium bromide 1613004 1613010 — 11.00
3.0% plus ethidium bromide 1613006 1613012 — 12.00
Mini ReadyAgarose Gels, TAE
1.0% 1613015 — 1613057 11.00
1.0% plus ethidium bromide 1613016 1613022 — 11.00
3.0% 1613017 — — 12.00
3.0% plus ethidium bromide 1613018 1613024 — 12.00
252 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Nucleic Acid Electrophoresis and Blotting
bio-rad.com/PFGE Pulsed Field Gel Electrophoresis
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 253
Nucleic Acid Electrophoresis and Blotting
Pulsed Field Gel Electrophoresis bio-rad.com/PFGE
Customization
Store up to 99 simple programs or 20 complex programs
■■
■■
ptimal resolution of both megabase- and kilobase-sized
O ■■
apid resolution of small fragments in the 100 bp–250 kb
R
DNA fragments range with FIGE and AFIGE technologies
■■
esolution of very large DNA molecules with secondary
R ■■
Expanded linear range of fragment separation to 50 –700 kb
pulses that release DNA caught in the gel matrix ■■
Enhanced resolution in selected fragment size ranges
For More Information
Web: bio-rad.com/chefXA
Request or download bulletin: 1906
Ordering Information
Catalog # Description Price, U.S. $
CHEF Mapper XA System*
1703670 CHEF Mapper XA System, 120 V, includes power module, embedded auto-algorithm for protocol optimization, Inquire
interactive algorithm program disk, electrophoresis cell, cooling module, variable-speed pump, Tygon tubing (12'),
14 x 13 cm (W x L) casting stand, 15-well 1.5 mm comb and comb holder, screened cap, disposable plug molds,
leveling bubble, cables, S. cerevisiae DNA size standards, two 0.5 A FB fuses, 5 g pulsed field Certified agarose,
5 g Certified megabase agarose, for North America
1703671 CHEF Mapper XA System, 100 V, for Japan Inquire
1703672 CHEF Mapper XA System, 220 V, for Asia Pacific/Europe Inquire
1703673 CHEF Mapper XA System, 240 V, for Asia Pacific/Europe Inquire
* All accessories are compatible with CHEF Mapper, CHEF-DR II, and CHEF-DR III systems. Accessories can be found on
page 256. A comprehensive listing of replacement parts can be found at bio-rad.com.
254 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Nucleic Acid Electrophoresis and Blotting
bio-rad.com/PFGE Pulsed Field Gel Electrophoresis
Ordering Information
Catalog # Description Price, U.S. $
CHEF-DR III Variable Angle System*
1703700 CHEF-DR III Variable Angle System, 120 V, includes power module, electrophoresis cell, cooling module Inquire
variable-speed pump, 14 x 13 cm casting stand with frame and platform, comb holder, 15-well 1.5 mm thick
comb, screened cap, disposable plug molds, 12' Tygon tubing, 2 plugs S. cerevisiae DNA size standards,
two 0.5 A FB fuses, 5 g pulsed field Certified agarose, 5 g Certified megabase agarose, for North America
1703702 CHEF-DR III Variable Angle System, 220/240 V, for Asia Pacific/Europe Inquire
1703703 CHEF-DR III Variable Angle System, 100 V, for Japan Inquire
* All accessories are compatible with CHEF Mapper, CHEF-DR II, and CHEF-DR III systems. Accessories can be found on
page 256. A comprehensive listing of replacement parts can be found at bio-rad.com.
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 255
Nucleic Acid Electrophoresis and Blotting
Pulsed Field Gel Electrophoresis bio-rad.com/PFGE
Ordering Information
Catalog # Description Price, U.S. $
CHEF-DR II Chiller System*
1703725 CHEF-DR II System, 120 V, includes electrophoresis cell, drive module, cooling module, control module, Inquire
variable-speed pump, 14 x 13 cm casting stand with frame and platform, comb holder, 15-well 1.5 mm
thick comb, screened cap, disposable plug molds, 12' Tygon tubing, 2 plugs S. cerevisiae DNA size
standards, 5 g pulsed field Certified agarose, 5 g Certified megabase agarose, for North America
1703727 CHEF-DR II System, 220/240 V, for Asia Pacific/Europe Inquire
1703728 CHEF-DR II System, 100 V, for Japan Inquire
Accessories for Chef Mapper, CHEF-DR III, and CHEF-DR II Systems*
1703654 Cooling Module, 120 V, for North America 4,070.00
1703688 Cooling Module, 100 V, for Japan 4,070.00
1703655 Cooling Module, 220/240 V, for Asia Pacific/Europe 4,070.00
1703644 Variable-Speed Pump, 120 V 935.00
1703648 Electrodes, thick gauge (0.02"), 6 345.00
1703711 Screened Caps, 5 55.00
1703713 50-Well Disposable Plug Molds, enough for 250 plugs 59.00
1703622 Reusable Plug Mold, 10 plug 180.00
1703689 Standard Casting Stand, includes 14 x 13 cm frame and platform 280.00
1703704 Wide/Long Combination Casting Stand, includes 21 x 14 cm frame and platform 430.00
1703699 Combination Comb Holder 165.00
1704326 10-Well Adjustable-Height Comb, 1.5 mm 57.00
1704325 10-Well Adjustable-Height Comb, 0.75 mm 57.00
1704324 15-Well Adjustable-Height Comb, 1.5 mm 57.00
1704323 15-Well Adjustable-Height Comb, 0.75 mm 57.00
1704322 20-Well Adjustable-Height Comb, 1.5 mm 57.00
1704344 30-Well Adjustable-Height Comb, 1.5 mm 57.00
1703627 15-Well Comb, 21 cm wide, 1.5 mm thick 105.00
1703628 30-Well Comb, 21 cm wide, 1.5 mm thick 110.00
1703645 45-Well Comb, 21 cm wide, 1.5 mm thick 105.00
1703623 Preparative Comb, 14 cm wide, 1.5 mm thick, with 2 outer wells for size standards 84.00
1704046 Leveling Table, 20 x 30 cm 360.00
1703643 Gel Scoop 80.00
* All accessories are compatible with CHEF Mapper, CHEF-DR III, and CHEF-DR II systems. A comprehensive listing of
replacement parts can be found at bio-rad.com.
256 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Nucleic Acid Electrophoresis and Blotting
bio-rad.com/PFGE Pulsed Field Gel Electrophoresis
Ordering Information
Catalog # Description Price, U.S. $
CHEF Genomic DNA Plug Kits
1703591 CHEF Mammalian Genomic DNA Plug Kit, contains 12 ml cell suspension buffer, 1.3 ml proteinase K, 30 ml 345.00
proteinase K reaction buffer, 12 ml 2% CleanCut agarose, 60 ml 10x wash buffer, screened cap, 2 disposable
plug molds; makes 100 plugs
1703592 CHEF Bacterial Genomic DNA Plug Kit, contains 12 ml cell suspension buffer, 1.3 ml proteinase K, 30 ml 455.00
proteinase K reaction buffer, 12 ml 2% CleanCut agarose, 60 ml 10x wash buffer, 1.6 ml lysozyme (25 mg/ml),
30 ml lysozyme buffer, screened cap, 2 disposable plug molds; makes 100 plugs
1703593 CHEF Yeast Genomic DNA Plug Kit, contains 12 ml cell suspension buffer, 1.3 ml proteinase K, 30 ml 525.00
proteinase K reaction buffer, 12 ml 2% CleanCut agarose, 60 ml 10x wash buffer, 1.6 ml lyticase, 25 ml lyticase
buffer, screened cap, 2 disposable plug molds; makes 100 plugs
1703594 CleanCut Agarose, 2%, 12 ml 30.00
Agaroses and Standards for Pulsed Field Gel Electrophoresis See Also
Pulsed field gel
electrophoresis
Bio-Rad offers a comprehensive line of agaroses for use with PFGE as well as CHEF DNA standards and convenient systems:
page 253.
buffers and reagents to simplify your PFGE experiments. See page 261 for agaroses, page 257 for size standards
Imaging systems:
and buffers. page 280.
Bioinformatics
Pulsed Field Standards software:
Bio-Rad offers standards for applications from FIGE separation of cosmid inserts to the largest chromosomal separations. page 292.
The higher MW standards are prepared in low-melt agarose blocks that can be cut to fit most well dimensions. Certified agaroses:
page 261.
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Nucleic Acid Electrophoresis and Blotting
Pulsed Field Gel Electrophoresis bio-rad.com/PFGE
48.5 kb
2,200 kb
1,600
38.4 5.7 Mb
3.13 Mb
33.5
1,125
29.9 4.6
1,020
945
24.8
14.7 kb 825
785 2.7
19.4 750 3.5
2.35
9.8 15.0
680
10.0 610
565 1.81
8.3 1.66
145.5 kb 450
365 1.37
97
48.5 285 1.05
225
4.9
5 kb Ladder 8–48 kb Ladder Lambda Ladder S. cerevisiae H. wingei S. pombe
Chromosomal DNA Chromosomal DNA Chromosomal DNA
Ordering Information
Catalog # Description Price, U.S. $
Agaroses and Standards for Pulsed Field Gel Electrophoresis
1613108 Certified Megabase Agarose, 25 g 89.00
1613109 Certified Megabase Agarose, 125 g 295.00
1613110 Certified Megabase Agarose, 500 g 930.00
1613100 Certified Molecular Biology Agarose, 25 g 61.00
1613101 Certified Molecular Biology Agarose, 125 g 180.00
1613102 Certified Molecular Biology Agarose, 500 g 580.00
1620137 Pulsed Field Certified Agarose, 100 g 235.00
1620138 Pulsed Field Certified Agarose, 500 g 1,045.00
Premixed Nucleic Acid Electrophoresis Buffers
1610733 10x Tris/Boric Acid/EDTA (TBE), 1 L 37.00
1610770 10x Tris/Boric Acid/EDTA (TBE), 5 L cube 110.00
1610743 50x Tris/Acetic Acid/EDTA (TAE), 1 L 84.00
1610773 50x Tris/Acetic Acid/EDTA (TAE), 5 L cube 245.00
Pulsed Field Standards*
1703624 CHEF DNA Size Standard, 5 kb ladder, 4.9–120 kb, 20–25 lanes 205.00
1703707 CHEF DNA Size Standard, 8–48 kb, 125 lanes 87.00
1703635 CHEF DNA Size Standard, lambda ladder, 0.05–1 Mb, 5 agarose blocks, sufficient for 25–40 plugs 415.00
Pulsed Field Markers*
1703605 CHEF DNA Size Marker, S. cerevisiae, 0.2–2.2 Mb, 5 agarose blocks, sufficient for 25–40 plugs 150.00
1703667 CHEF DNA Size Marker, H. wingei, 1–3.1 Mb, 5 agarose blocks, sufficient for 25–40 plugs 160.00
1703633 CHEF DNA Size Marker, S. pombe, 3.5–5.7 Mb, 5 agarose blocks, sufficient for 25–40 plugs 160.00
* CHEF, clamped homogeneous electrical field. For more information, see page 253–257.
258 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Nucleic Acid Electrophoresis and Blotting
bio-rad.com/nastains Buffers and Reagents for Nucleic Acid Electrophoresis
Ordering Information
Catalog # Description Price, U.S. $
1610433 Ethidium Bromide Solution, 10 mg/ml, 10 ml 40.00
Tracking Dyes
Ordering Information
Catalog # Description Price, U.S. $
1610404 Bromophenol Blue, 10 g 40.00
1610423 Xylene Cyanol FF, 25 g 120.00
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 259
Nucleic Acid Electrophoresis and Blotting
Buffers and Reagents for Nucleic Acid Electrophoresis bio-rad.com/dnareagents
Ordering Information
Catalog # Description Price, U.S. $
1665111 UView 6x Loading Dye, 0.2 ml 31.25
1665112 UView 6x Loading Dye, 1 ml 95.00
Ordering Information
Catalog # Description Price, U.S. $
Premixed Nucleic Acid Sample Loading Buffers
1610767 5x Nucleic Acid Sample Buffer, 10 ml 20.00
1610768 1x TBE-Urea Sample Buffer, 30 ml 56.00
Premixed Nucleic Acid Electrophoresis Buffers
1610773 50x Tris/Acetic Acid/EDTA (TAE), 5 L cube 245.00
1610770 10x Tris/Boric Acid/EDTA (TBE), 5 L cube 110.00
1610741 10x Tris/Boric Acid/EDTA (TBE), extended range, 1 L bottle 57.00
1610743 50x Tris/Acetic Acid/EDTA (TAE), 1 L bottle 84.00
1610733 10x Tris/Acetic Acid/EDTA (TBE), 1 L bottle 37.00
260 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Nucleic Acid Electrophoresis and Blotting
bio-rad.com/agarose Buffers and Reagents for Nucleic Acid Electrophoresis
Ordering Information
Description 5 x 1 ml Price, U.S. $ 1 x 5 ml Price, U.S. $ 5 x 5 ml Price, U.S. $
Certified Agaroses for Standard Applications
Certified molecular biology agarose 1613100 61.00 1613101 180.00 1613102 580.00
Certified PCR agarose 1613103 140.00 1613104 350.00 1613105 1,475.00
Certified low range ultra agarose 1613106 145.00 1613107 445.00 —
Certified megabase agarose 1613108 89.00 1613109 295.00 1613110 930.00
Certified low-melt agarose 1613111 170.00 1613112 490.00 —
Certified PCR low-melt agarose 1613113 150.00 1613114 435.00 1613115 1,630.00
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Nucleic Acid Electrophoresis and Blotting
DNA Ladders bio-rad.com/dnaladders
DNA Ladders
See Also Bio-Rad offers a broad variety of DNA ladders for conventional DNA gel electrophoresis, including molecular rulers
Pipet tips:
with evenly spaced banding patterns and EZ Load™ rulers premixed with loading buffer.
page 405.
Micro test tubes: DNA Ladder Selection Guide
page 409.
Type Description
ReadyAgarose
precast gels: Molecular Rulers
page 252. Standard and EZ Load molecular rulers DNA ladders of even base pair length increments, available in 5 size ranges
Imaging systems: AmpliSize® molecular ruler Blunt-end DNA of precise length and known sequence
page 280.
Molecular Mass Rulers
DNA gel
electrophoresis
Standard and EZ Load molecular mass rulers Multiple bands of defined mass ranging from 10–100 ng for DNA quantitation
systems:
page 244.
Molecular Rulers Selection Guide
Number of
Ruler Concentration Range Number of Bands Reference Band Amount Suggested Gel Type Applications
20 bp 0.2 µg/µl 20–1,000 bp 50 in 20 bp 200 bp 50 µg DNA 2.5–4% agarose 100
EZ Load 20 bp 0.1 µg/µl increments
100 bp 0.1 µg/µl 100–1,000 bp 10 in 100 bp None 25 µg DNA 2.5–4% agarose 100
EZ Load 100 bp 0.05 µg/µl increments
100 bp PCR 0.2 µg/µl 100–3,000 bp 30 in 100 bp 1,000 bp and 40 µg DNA 0.8–3% agarose 100
EZ Load 100 bp PCR 0.08 µg/µl increments 3,000 bp
500 bp 0.2 µg/µl 500–8,000 bp 16 in 500 bp 5,000 bp 40 µg DNA 0.8–1% agarose 100
EZ Load 500 bp 0.08 µg/µl increments
1 kb 0.2 µg/µl 1–15 kb 15 in 1 kb 5 kb 40 µg DNA 0.8–1% agarose 100
EZ Load 1 kb 0.08 µg/µl increments
2.5 kb 0.1 µg/µl 2.5–35 kb 14 in 2.5 kb 10 kb 40 µg DNA 0.8% agarose 100
increments
AmpliSize 0.1 µg/µl 50–2,000 bp 10 None 25 µg DNA 1.5–3% agarose 50
(10 ng/band/µl)
Precision 0.1 µg/µl 100–1,000 bp 5, from 10–100 ng None 25 µg DNA 1–3% agarose 100
EZ Load precision 0.05 µg/µl 100–1,000 bp 5, from 10–100 ng None 25 µg DNA 1–3% agarose 100
Molecular
Molecular Rulers Mass Rulers
1,000 bp 1,000 bp 15 kb
900 35 kb
800 8,000 bp 2,000 bp 1,000 bp
700 7,000 10 1,500
600 6,000 8.0
3,000 bp 5,000 1,000 700
500 4,000 6.0 700 500
3,500
400 3,000 5.0
500
2,500 4.0
300 10 400 200
200 2,000 300 100
180 1,000 3.0
160
140 200 1,500 200
120
Precision, EZ Load
100 2.0
1,000 Precision Mass
80
100 Rulers
60 100 2.5
40 50
100 500
1.0
20
20 bp and 100 bp and 100 bp PCR and 500 bp and 1 kb and 2.5 kb AmpliSize
EZ Load 20 bp EZ Load 100 bp EZ Load 100 bp EZ Load 500 bp EZ Load 1 kb Ruler Ruler
Rulers Rulers PCR Rulers Rulers Rulers
262 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Nucleic Acid Electrophoresis and Blotting
bio-rad.com/dnaladders DNA Ladders
Molecular Rulers
Ordering Information
Catalog # Description Price, U.S. $
20 bp Molecular Rulers
1708201 20 bp Molecular Ruler, 250 µl, 20–1,000 bp, 100 applications 115.00
1708351 EZ Load 20 bp Molecular Ruler, 500 µl, 20–1,000 bp, includes 1 ml 5x nucleic acid sample buffer, 115.00
100 applications
100 bp Molecular Rulers
1708202 100 bp Molecular Ruler, 250 µl, 100–1,000 bp, 100 applications 115.00
1708352 EZ Load 100 bp Molecular Ruler, 500 µl, 100–1,000 bp, includes 1 ml 5x nucleic acid sample buffer, 115.00
100 applications
1708206 100 bp PCR Molecular Ruler, 200 µl, 100–3,000 bp, 100 applications 115.00
1708353 EZ Load 100 bp PCR Molecular Ruler, 500 µl, 100–3,000 bp, includes 1 ml 5x nucleic acid sample buffer, 115.00
100 applications
500 bp Molecular Rulers
1708203 500 bp Molecular Ruler, 200 µl, 500–8,000 bp, 100 applications 115.00
1708354 EZ Load 500 bp Molecular Ruler, 500 µl, 500–8,000 bp, includes 1 ml 5x nucleic acid sample buffer, 115.00
100 applications
1 kb Molecular Rulers
1708204 1 kb Molecular Ruler, 200 µl, 1–15 kb, 100 applications 93.00
1708355 EZ Load 1 kb Molecular Ruler, 500 µl, 1–15 kb, includes 1 ml 5x nucleic acid sample buffer, 100 applications 93.00
2.5 kb Molecular Ruler
1708205 2.5 kb Molecular Ruler, 400 µl, 2.5–35 kb, 100 applications 165.00
AmpliSize Molecular Ruler
1708200 AmpliSize Molecular Ruler, 250 µl, 50–2,000 bp, 50 applications 165.00
Bio-Rad’s precision molecular mass rulers are DNA markers that allow accurate DNA quantitation in gels, making them
ideal for densitometry or image analysis. These ladders have five bands, which contain 100, 70, 50, 20, and 10 ng of DNA.
The EZ Load™ precision molecular mass ruler has been blended with sample loading buffer and is ready to load.
Ordering Information
Catalog # Description Price, U.S. $
1708207 Precision Molecular Mass Ruler, 250 µl, 100–1,000 bp, 10–100 ng, 100 applications 115.00
1708356 EZ Load Precision Molecular Mass Ruler, 500 µl, 100–1,000 bp, 10–100 ng, 100 applications 115.00
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Nucleic Acid Electrophoresis and Blotting
Northern and Southern Blotting bio-rad.com/dnaelectro
Ordering Information
Catalog # Description Price, U.S. $
Blot Transfer and Processing Buffers
1610774 20x SSC, 1 L 22.00
1610775 20x SSC, 5 L cube 97.00
Mutation Analysis
264 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Nucleic Acid Electrophoresis and Blotting
bio-rad.com/dnaelectro Mutation Analysis
Electrophoresis tank
Ordering Information
Catalog # Description Price, U.S. $
DCode Systems*
1709080 DCode System for DGGE, 120 V, for 16 cm gels with single prep well (1 mm), includes comb gasket, 7,765.00
2 sets of clamps, Model 475 gradient former, all parts required to cast gradient gels
1709081 DCode System for DGGE, 220/240 V, for 16 cm gels with single prep well (1 mm) 7,765.00
1709082 DCode System for DGGE, 100 V, for 16 cm gels with single prep well (1 mm) 7,535.00
1709088 DCode System for CDGE, 100 V, for 16 cm gels with 20 wells (1 mm) 7,140.00
1709091 DCode System for TTGE, 100 V, for 16 cm gels with 20 wells (1 mm) 7,310.00
1709105** Complete DCode System, 120 V, PC, for all gel sizes and types described above, includes software, Inquire
standard and cooling tanks, Model 475 gradient former, sandwich clamps, pressure clamp, comb gasket
and holder, fittings required for gradient gels
continues
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 265
Nucleic Acid Electrophoresis and Blotting
Mutation Analysis bio-rad.com/dnaelectro
Ordering Information
Catalog # Description Price, U.S. $
Adaptor Kits***
1709125 GGE Kit, for 16 cm gels with single prep well (1 mm), includes sandwich clamps, pressure clamp,
D 960.00
comb gasket and holder, fittings required for gradient gel casting
1709126 DGGE Kit, for 10 cm gels with 2 prep wells (1 mm) 960.00
1709127 CDGE/TTGE Kit, for 16 cm gels with 20 prep wells (1 mm) 540.00
1709128 Complete SSCP Kit, for 20 cm gels with 20 wells (0.75 mm), includes sandwich clamps, 1,055.00
cooling finger adaptor for use with external chiller
Accessories†
1709240 WinMelt Software, PC/Windows 1,000.00
1709241 Interactive CD-ROM Training Guide 170.00
1709042 Model 475 Gradient Delivery System, includes cam-operated manual gradient former, 2 each of 10 and 30 ml 1,025.00
syringes, all accessories required to cast gradient gels
Electrophoresis Reagents and DNA Control Reagents
1709150 DCode Control Reagent Kit for DGGE/CDGE/TTGE, includes primers (one GC-clamped) and DNA templates 490.00
for production of wild-type and mutant DNA
1709151 DCode Control Reagent Kit for SSCP, includes primers and DNA templates for production of wild-type 490.00
and mutant DNA
1709170 DCode Electrophoresis Reagent Kit for DGGE, includes 500 ml 40% acrylamide/bis (37.5:1), 2 x 1 L 50x TAE 540.00
buffer, 225 ml 100% deionized formamide, 10 ml 10 mg/ml ethidium bromide, 10 ml DCode dye solution, 5 ml
TEMED, 1 ml 2x gel loading dye, 10 g ammonium persulfate
* Each system includes electrophoresis/temperature control module, sandwich core, kit to cast gels of indicated size and type
(2 sets of plates, 2 sets of clamps and spacers, 2 combs), control reagents for indicated application(s).
** For PC, includes WinMelt software.
*** Each kit includes 2 sets of plates, 2 sets of spacers, 2 combs.
† For a complete list of accessories, including combs and spacers, for the DCode system, go to bio-rad.com/dcode.
266 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Experion Automated Electrophoresis System
bio-rad.com/experion
■■
Digital data storage for easy record keeping and reporting Note: The Experion automated electrophoresis system will be
discontinued in 2016. Analysis kits and support for this system will
■■
Flexible and easy export options and annotation ability
continue to be available through 2021.
for publications, reports, and presentations
1.5
System
peak Eluted DHFR
A 280
0.5
0.0
0 5 10 15 Results
Run volume, ml table
Analysis of chromatographic fractions with the Experion system. Left, chromatogram showing purification of histidine-tagged DHFR using
Profinity™ IMAC resin and the BioLogic DuoFlow™ system. Right, comparison of analysis of fractions using the Experion system and SDS-PAGE
(shown as inset). The Experion system generated an electropherogram and virtual gel image of the separation. Note that the virtual gel image is
comparable to the SDS-PAGE gel image. The Experion system also automatically reports the size (MW), relative concentration, and percent of each
resolved protein in the total sample in the results table.
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 267
Experion Automated Electrophoresis System
bio-rad.com/experion
Ordering Information
Catalog # Description Price, U.S. $
7007030 Experion Priming Station, 100–240 V, includes 2 priming seals 1,325.00
7007031 Experion Priming Seals, replacement, provides air seal on top of priming well, 2 28.00
Ordering Information
Catalog # Description Price, U.S. $
7007043 Experion Vortex Station II, 100–240 V, for preparing Experion RNA/DNA chips 995.00
268 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Experion Automated Electrophoresis System
bio-rad.com/experion
Experion™ Software
Experion software adds to the efficiency of the Experion Experion Security Edition Software (Optional)
automated electrophoresis system. Results are displayed The optional Security Edition offers tools for compliance
with peak electropherograms, in a virtual gel view, and with U.S. FDA 21 CFR Part 11 regulations:
as results tables. Additionally, versions 3.0 and above ■■
Different levels of access to different software functions
automatically generate a validated RNA quality indicator (RQI)
number that correlates with eukaryotic total RNA sample
■■
Audit trail table tracks daily use of the system
Key Features
■■
Electronic signatures facilitate record keeping and tracking
Real-time display of data acquisition
■■ ■■
eport generation enables quick viewing and archiving
R
■■
Manual integration of peaks of multiple run parameters, data, audit trail, and
electronic signatures
■■
Automatic sizing, quantitation, and % total calculations
For More Information
■■
Statistical evaluations (mean, std deviation, and %CV) Web: bio-rad.com/experionsoftware
■■
Multiple protein quantitation method options Request or download bulletins: 3171 and 5761
■■
Flexible printing and data export options
Note: The Experion automated electrophoresis system will be
discontinued in 2016. Analysis kits and support for this system will
Experion Validation Kit (Optional) continue to be available through 2021.
The IQ/OQ validation kit includes automated protocols
that test the critical functions of the system to verify System Requirements
and validate the system to the specified functionality. Operating system Windows XP (Service Pack 3), Windows Vista
Validation should be performed at least biannually, when (Service Pack 1), WIndows 7 (32 bit)
Processor (CPU) Pentium 4 (3 GHz processor) PC only
troubleshooting, and after moving the instrument.
RAM 1 GB
Hard drive space 80 GB
USB 2.0 1 port
Other drives CD-ROM
Ordering Information
Catalog # Description Price, U.S. $
7007050 Experion Software, system operation and standard data analysis tools, includes software CD-ROM 1,350.00
7007051 Experion Validation Kit, includes 3 test chips, qualification procedures, dongle for PC 1,390.00
7007052 Experion Software, Security Edition, standard and 21 CFR Part 11 compliance data analysis tools, 4,280.00
includes 3 test chips, qualification procedures, dongle for PC
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 269
Experion Automated Electrophoresis System
bio-rad.com/experion
Specifications
DNA 1K = C3
12K = C1
DNA 1K = C3
12K = C1
DNA 1K = C3
12K = C1
DNA 1K = C3
12K = C1
DNA 1K = C3
12K = C1
LOT# JM27UP02 LOT# JJ29UK01 LOT# KA31UK01 LOT# LOT#
JJ05UK02
JM27UP02 LOT# JJ29UK01 LOT# KA31UK01 LOT# JJ05UK02 LOT# JM27UP02 LOT# JJ29UK01 LOT# KA31UK01 LOT# JJ05UK02 LOT# JM27UP02 LOT# JJ29UK01 LOT# KA31UK01 LOT# JJ05UK02 LOT# JM27UP02 LOT# JJ29UK01 LOT# KA31UK01 LOT# JJ05UK02
EXP JUN/25/08 EXP MAR/31/08 EXP MAY/01/09 EXP MAR/05/08
EXP JUN/25/08 EXP MAR/31/08 EXP MAY/01/09 EXP MAR/05/08 EXP JUN/25/08 EXP MAR/31/08 EXP MAY/01/09 EXP MAR/05/08 EXP JUN/25/08 EXP MAR/31/08 EXP MAY/01/09 EXP MAR/05/08 EXP JUN/25/08 EXP MAR/31/08 EXP MAY/01/09 EXP MAR/05/08
Pro260 Chip RNA HighSens Chip RNA StdSens Chip DNA 1K Assay DNA 12K Assay
Number of samples 1–10 1–11 1–12 1–11 1–11
Sample volume 4 µl 1 µl 1 µl 1 µl 1 µl
Linear dynamic range 5–2,000 ng/µl BSA — — — —
Concentration range 5–2,000 ng/µl 100–5,000 pg/µl 5–500 ng/µl 0.1–50 ng/µl 0.1–50 ng/µl
Separation range 10–260 kD — — 15–1,500 bp 50–17,000 bp
Sensitivity 2.5 ng/µl of carbonic 100 pg 5 ng 0.1 ng 0.1 ng
anhydrase in 1x PBS
270 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Experion Automated Electrophoresis System
bio-rad.com/experion
Ordering Information
Catalog # Description Price, U.S. $
7007101 xperion Pro260 Analysis Kit for 10 Chips, includes 10 Pro260 chips, 1 cleaning chip, 3 x 520 µl Pro260 gel,
E 405.00
45 µl Pro260 stain, 60 µl Pro260 ladder (10–260 kD), 400 µl Pro260 sample buffer, 3 spin filters
7007102 Experion Pro260 Analysis Kit for 25 Chips, includes 25 Pro260 chips, 1 cleaning chip, 5 x 520 µl Pro260 gel, 785.00
2 x 45 µl Pro260 stain, 2 x 60 µl Pro260 ladder (10–260 kD), 2 x 400 µl Pro260 sample buffer, 5 spin filters
7007103 Experion RNA StdSens Analysis Kit for 10 Chips, includes 10 RNA StdSens chips, 2 cleaning chips, 390.00
1,250 µl RNA gel, 20 µl RNA StdSens stain, 20 µl RNA ladder, 900 µl RNA StdSens loading buffer, 2 spin filters
7007104 Experion RNA StdSens Analysis Kit for 25 Chips, includes 25 RNA StdSens chips, 2 cleaning chips, 760.00
2 x 1,250 µl RNA gel, 2 x 20 µl RNA StdSens stain, 2 x 20 µl RNA ladder, 2 x 900 µl RNA StdSens
loading buffer, 4 spin filters
7007105 Experion RNA HighSens Analysis Kit for 10 Chips, includes 10 RNA HighSens chips, 2 cleaning 405.00
chips, 1,250 µl RNA gel, 20 µl RNA HighSens stain, 20 µl RNA ladder, 900 µl RNA HighSens loading
buffer, 100 µl RNA sensitivity enhancer, 2 spin filters
7007106 Experion RNA HighSens Analysis Kit for 25 Chips, includes 25 RNA HighSens chips, 2 cleaning chips, 785.00
2 x 1,250 µl RNA gel, 2 x 20 µl RNA HighSens stain, 20 µl RNA ladder, 2 x 900 µl RNA HighSens loading
buffer, 2 x 100 µl RNA sensitivity enhancer, 4 spin filters
7007107 Experion DNA 1K Analysis Kit for 10 Chips, includes 10 DNA chips, 1 cleaning chip, 3 x 250 µl DNA 1K gel, 480.00
40 µl DNA 1K stain, 20 µl DNA 1K ladder, 750 µl DNA 1K loading buffer, 3 spin filters
7007108 Experion DNA 12K Analysis Kit for 10 Chips, includes 10 DNA chips, 1 cleaning chip, 650 µl DNA 12K gel, 480.00
40 µl DNA 12K stain, 20 µl DNA 12K ladder, 750 µl DNA 12K loading buffer, 3 spin filters
7007307 Experion DNA 1K Analysis Kit for 30 Chips, includes 30 DNA chips, 3 cleaning chips, 9 x 250 µl DNA 1K gel, 1,285.00
3 x 40 µl DNA 1K stain, 3 x 20 µl DNA 1K ladder, 3 x 750 µl DNA 1K loading buffer, 9 spin filters
7007308 Experion DNA 12K Analysis Kit for 30 Chips, includes 30 DNA chips, 3 cleaning chips, 3 x 650 µl DNA 1,285.00
12K gel, 3 x 40 µl DNA 12K stain, 3 x 20 µl DNA 12K ladder, 3 x 750 µl DNA 12K loading buffer, 9 spin filters
Experion Analysis Kit Accessories
7007151 Experion Pro260 Chips, 10, plus 1 cleaning chip 330.00
7007152 Experion Pro260 Reagents and Supplies for 10 Chips, includes 3 x 520 µl Pro260 gel, 45 µl Pro260 stain, 230.00
60 µl Pro260 ladder (10–260 kD), 400 µl Pro260 sample buffer, 3 spin filters
7007153 Experion RNA StdSens Chips, 10, plus 2 cleaning chips 330.00
7007154 Experion RNA StdSens Reagents and Supplies for 10 Chips, includes 1,250 µl RNA gel, 245.00
20 µl RNA StdSens stain, 20 µl RNA ladder, 900 µl RNA StdSens loading buffer, 2 spin filters
7007155 Experion RNA HighSens Chips, 10, plus 2 cleaning chips 330.00
7007156 Experion RNA HighSens Reagents and Supplies for 10 Chips, includes 1,250 µl RNA gel, 20 µl RNA 260.00
HighSens stain, 20 µl RNA ladder, 900 µl RNA HighSens loading buffer, 100 µl RNA sensitivity enhancer,
2 spin filters
7007163 Experion DNA Chips, 10, for DNA 1K and 12K analyses, plus 1 cleaning chip 325.00
7007164 Experion DNA 1K Reagents and Supplies for 10 Chips, includes 3 x 250 µl DNA 1K gel, 40 µl DNA 1K stain, 300.00
20 µl DNA 1K ladder, 750 µl DNA 1K loading buffer, 3 spin filters
7007165 Experion DNA 12K Reagents and Supplies for 10 Chips, includes 650 µl DNA 12K gel, 40 µl DNA 12K stain, 385.00
20 µl DNA 12K ladder, 750 µl DNA 12K loading buffer, 3 spin filters
7007251 Experion Cleaning Chips, 10 135.00
7007252 Experion Electrode Cleaner, 250 ml 50.00
7007253 Experion DEPC-Treated Water, 100 ml 89.00
7007254 Experion Spin Filters, 10 61.00
7007255 Experion RNA Ladder, 20 µl 50.00
7007256 Experion Pro260 Ladder, 60 µl 77.00
7007261 Experion DNA 1K Ladder, 20 µl 60.00
7007262 Experion DNA 12K Ladder, 20 µl 60.00
7007112 Experion Mouse Liver Total RNA Standard, 500 ng/µl, 20 µl 36.00
5000208 Bovine Gamma Globulin (BGG) Standard, 2 mg/ml, 2 ml 81.00
7007264 Cleaning Swabs, lint free, for electrode deep cleaning, 25 40.00
7007270 Experion Pro260 Sample Buffer, 400 µl, 2 vials 145.00
1632091 ReadyPrep Proteomics Grade Water, 500 ml 40.00
1610710 2-Mercaptoethanol, 25 ml 23.00
1610610 Dithiothreitol (DTT), 1 g 27.00
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Experion Automated Electrophoresis System
bio-rad.com/experion
Ordering Information
Catalog # Description Price, U.S. $
7007110 xperion Pro260 Starter Kit, includes 3 Experion chips, 1 cleaning chip, Experion reagents, spin filters,
E 640.00
IgG protein standard, DTT, cleaning swabs (lint free), electrode cleaner, narrow bore polypropylene pipet tips,
polypropylene 0.5 ml microcentrifuge tubes, DEPC-treated water (0.2 µm filtered)
7007111 Experion RNA StdSens Starter Kit, includes 3 Experion chips, 2 cleaning chips, Experion reagents, 640.00
spin filters, total RNA standard, cleaning swabs (lint free), electrode cleaner, narrow bore polypropylene pipet tips,
RNase- and DNase-free polypropylene 0.5 ml microcentrifuge tubes, DEPC-treated water (0.2 µm filtered)
272 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Electrophoresis and Blotting
Subject Index
Subject Index
2-D Premixed Buffers and Individual Reagents, 208 Model 175 Tube Gel Accessories, 211
2-D SDS-PAGE Standards, 163 Model 422 Electro-Eluter, 217
Agaroses and Standards for Pulsed Field Gel Electrophoresis, 257 Model 491 Prep Cell and Mini Prep Cell, 215
Bio-Dot® and Bio-Dot SF Microfiltration Apparatus, 230 Model 583 and HydroTech™ Gel Drying Systems, 218
Bio-Lyte® Ampholytes, 198 Molecular Mass Rulers, 263
Blotting-Grade Conjugates and Reagents, 242 Molecular Rulers, 263
Blotting Stains and Tracking Dyes, 235 Nitrocellulose Membranes , 232
Certified™ Agaroses, 261 PowerPac™ Adaptor, 156
CHEF-DR® II Chiller System, 256 PowerPac™ Basic Power Supply, 155
CHEF-DR® III Variable Angle System, 255 PowerPac™ HC High-Current Power Supply, 155
CHEF Genomic DNA Plug Kits, 257 PowerPac™ HV High-Voltage Power Supply, 155
CHEF Mapper® XA System, 254 PowerPac™ Universal Power Supply, 156
Clarity™ Western ECL Substrate, 238 Precision Plus Protein™ Standards, 158
Cleaning Concentrate, 198 Premixed Blotting Buffers, 264
Colorimetric AP Detection, 241 Premixed Blotting Buffers and Buffer Reagents, 236
Colorimetric HRP Detection, 239 Premixed Gel-Casting Buffers, 193
Combs for Wide Mini-Sub® Cell and Sub-Cell® GT Cells, 249 Premixed Running Buffers, 192
Coomassie Stains, 199 Premixed Sample Loading and Running Buffers, 260
Criterion™ Accessories, 182 Premixed Sample Loading Buffers, 192
Criterion™ Blotter, 227 Prestained SDS-PAGE Standards, 161
Criterion™ Cell and Criterion™ Dodeca™ Cell, 175 PROTEAN® i12™ IEF System, 202
Criterion™ Precast Gels, 177 PROTEAN® IEF Accessories, 204
Cross-Linkers and Catalysts, 196 PROTEAN® II Multi-Gel Casting Chamber, 188
DCode™ Universal Mutation Detection System, 264 PROTEAN® II xi 2-D Tube Gel Cell, 210
Detergents, 197 PROTEAN® II xi and XL Accessories, 186
Ethidium Bromide Solution, 259 PROTEAN® II xi and XL Cells, 184
Experion™ Analysis Kits, 270 PROTEAN® II xi and XL Multi-Cells, 186
Experion™ Priming Station, 268 PROTEAN® Plus Dodeca™ Cell, 189
Experion™ Software, 269 PVDF Membranes, 233
Experion™ Starter Kits, 272 ReadyAgarose™ Precast Gel System, 252
Experion™ Vortex Station II, 268 Ready Gel® Precast Gels, 171
Filter Paper, 235 ReadyPrep™ 2-D Starter Kit, 207
Fluorescent Stains, 201 ReadyStrip™ IPG Strips, 205
GelAir™ Drying System, 220 Rotofor® Cell, Mini Rotofor Cell Accessories, and MicroRotofor™, 214
Gel-Casting Reagents, 195 Silver Stains, 200
Gel Drying Solution, 198 Sub-Cell® GT Cell, 248
IEF Standards, 163 Sub-Cell® Model 96 Cell, 250
IEP/IEF Agaroses, 196 Sub-Cell® Model 192 Cell, 251
Immun-Star™ AP Chemiluminescence Kits, 239 TGX™ Handcast Acrylamide Solutions, 194
Kaleidoscope™ Standards, 161 Total Protein Stains, 243
Large-Format Electrophoresis Accessories, 190 Tracking Dyes, 197, 259
Mini Incubation Trays, 231 Trans-Blot® Cell, 228
Mini-PROTEAN® 2-D Electrophoresis Cell, 209 Trans-Blot® Plus Cell, 229
Mini-PROTEAN® 3 Dodeca™ Cell, 167 Trans-Blot® SD Semi-Dry Transfer Cell, 225
Mini-PROTEAN® Hand Casting Accessories, 173 Trans-Blot® Turbo™ Transfer System, 224
Mini-PROTEAN® II Multiscreen Apparatus, 231 Unstained SDS-PAGE Standards, 162
Mini-PROTEAN® Precast Gels, 168 UView™ 6x Loading Dye, 259
Mini-PROTEAN® Tetra Cell, 164 Whole Gel Eluter and Mini Whole Gel Eluter Accessories, 217
Mini-Sub® Cell GT Cells, 245 Wide Mini-Sub® Cell GT Cells, 247
Mini Trans-Blot® Cell, 226 Zeta-Probe® Nylon Membranes, 234
Model 111 Mini IEF Cell, 212
In the U.S., call toll free at 1-800-424-6723 Electrophoresis and Blotting | 273
274 | Electrophoresis and Blotting In the U.S., call toll free at 1-800-424-6723
Antibodies
Antibodies 276
Antibodies
abdserotec.com
Antibodies
See Also Bio-Rad offers an extensive selection of antibodies Antibodies are highly specific and come with a
SureBeads
commercialized through AbD Serotec®, a Bio-Rad company. performance guarantee. The following data show two of
magnetic the many highly specific antibody clones that are unique to
beads system: More than 11,000 antibodies, kits, reagents, standards,
page 2.
AbD Serotec.
and controls are available for research use. Specificity
and performance are hallmarks of the antibody collection.
Antibodies can be used for many applications, including: 105
■■
Western blotting
■■
Flow cytometry 104
■■
Immunohistochemistry
Enzyme-linked immunosorbent assay
CD11b
■■
103
■■
Immunofluorescence
Antibodies are available for the following research areas: 102
■■
Innate immunity
■■
Veterinary immunology 101
■■
Cell biology
■■
Apoptosis and autophagy 101 102 103 104 105
■■
Microbiology F4/80
■■
Neuroscience
Flow cytometry analysis of anti-mouse F4/80, a macrophage
■■
Immunology marker. Forward scatter and side scatter gated mouse peritoneal
■■
Stem cell research macrophages were dual stained with F4/80-FITC conjugated at a
■■
Endocrinology 1/5 dilution and CD11b–Alexa Fluor 647 conjugated at a 1/5 dilution.
The isotype control pair is shown in red. Fc receptors were blocked
Antibodies conjugated to over 20 dyes, including Alexa by mouse seroblock.
Fluor, DyLight, fluorescein isothiocyanate (FITC), and
phycoerythrin (PE), for multicolor flow cytometry are
also available.
In addition, Bio-Rad offers the PrecisionAb™ antibody
product line, which is exclusively validated for western
blotting (see page 277).
HuCAL® Technology
AbD Serotec uses the HuCAL technology to offer a custom
service for the in vitro generation of fully human monoclonal
antibodies. With this technique, specific Fab antibodies
can be generated within 8 weeks, with a greater than 90%
success rate and without the immunization of animals.
The HuCAL PLATINUM® library contains more than 45 Immunoperoxidase staining of anti-rat CD68, a macrophage
billion functional human antibody specificities already marker. A rat lymph node cryosection was probed with mouse
anti-rat CD68 antibody, clone ED1, followed by horseradish
prefabricated in an engineered Fab format. The technology
peroxidase–conjugated goat anti-mouse IgG1 antibody.
uses CysDisplay®, a novel and proprietary phage display
method that allows for the selection of high-affinity For More Information
antibodies that are expressed on the surface of filamentous Web: abdserotec.com
phage. Antibodies can be customized to specific needs.
.7
■■
93
64
3
4
2
validated to ensure that it exhibits low nonspecific binding
s
3T
LT-
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K2
W2
t
F7
mo
rka
La
2
49
6
NIH
MO
MC
RA
HE
C6
He
He
K5
Ra
A5
igh sensitivity — all antibodies must be able to detect
Ju
■■
H kD
the protein of interest at endogenous levels
■■
eproducible performance — stringent QC procedures
R 250
ensure consistent lot-to-lot performance 150
■■
rial kits for every antibody — each PrecisionAb
T 100
Antibody is available in a convenient 2–western blot 75
format for a cost-effective way to assess antibody 50
performance 37
■■
ositive control lysate — positive control lysate is
P 25
provided to facilitate target band identification and 20
15
troubleshooting of experimental procedures 10
■■
ull antibody validation results and protocol —
F
Antibodies are validated against 12 whole-cell lysates
the entire western blot is displayed for a complete picture (not overexpressed proteins). This blot represents one such
of antibody performance; a detailed validation protocol is validation, with the arrow indicating the presence of CDK2 protein
provided to help optimize experimental conditions in the various cell lysates.
Imaging Systems
Imaging systems detect images and quantitate colorimetric, chemiluminescent, and fluorescent signals. Bio-Rad offers
software (page 292) that provides automation for image acquisition with data analysis and validation. Refer to the guide
below to select the imaging system best suited for your applications.
Learn More about the Technology
Web: bio-rad.com/tech/proteinimaging
Ordering Information
Catalog # Description Price, U.S. $
1708370 hemiDoc Touch Imager, includes internal computer, 12" touch screen display, camera, Image Lab software,
C Inquire
chemi/UV/stain-free sample tray (#1708374); other sample trays available separately
1709690 Image Lab Software, stand-alone software, for 1-D analysis, compatible with PC or Mac 1,085.00
1708381 ChemiDoc Touch V3 Western Workflow for Mini Gels, includes ChemiDoc Touch imager with Image Lab Inquire
software, UV/stain-free sample tray, 50 Mini-PROTEAN TGX Any kD Stain-Free precast gels, SDS-PAGE
accessories, Mini-PROTEAN Tetra cell, Trans-Blot Turbo starter kit, 50 PVDF transfer packs for mini gels
1708382 ChemiDoc Touch V3 Western Workflow for Midi Gels, includes ChemiDoc Touch imager with Image Lab Inquire
software, UV/stain-free sample tray, 50 4–20% Criterion TGX Stain-Free precast gels, SDS-PAGE accessories,
Criterion cell, Trans-Blot Turbo starter kit, 50 PVDF transfer packs for midi gels
Accessories
1708372 hite Sample Tray, for gels stained with Coomassie Blue, copper, silver, or zinc stains
W 800.00
1708373 Blue Sample Tray, for gels stained with GelGreen or any SYBR stain 800.00
1708374 Chemi/UV/Stain-Free Sample Tray, for chemiluminescent blots, stain-free gels/blots, and gels stained with 800.00
ethidium bromide, SYPRO Ruby, Oriole, GelRed, and SYBR stains
1708375 UV Safety Shield, to protect against UV light exposure during band excision 250.00
1708376 Gel Alignment Templates, for consistent placement of gels and blots 50.00
1708377 Holder for ChemiDoc Touch Sample Trays and UV Shield, pkg of 1, holds 3 sample trays and 1 UV shield; 75.00
trays and shield not included
1708378 ChemiDoc Touch IQ/OQ Protocols, protocols for installation qualification/operational qualification Inquire
1708380 ChemiDoc Touch Leveling Feet, ensures level imaging stage Inquire
Ordering Information
Catalog # Description Price, U.S. $
1708280 hemiDoc MP Imaging System with Image Lab Software, compatible with PC or Mac, includes darkroom,
C Inquire
UV transilluminator, epi-white illumination, camera, power supply, cables, Image Lab software
1708283 ChemiDoc MP Red LED Module Kit, for use with applications requiring red fluorophore detection, 3,185.00
includes 2 epi-red LED modules, 1 red emission filter
1708284 ChemiDoc MP Green LED Module Kit, for use with applications requiring green fluorophore detection, 3,185.00
includes 2 epi-green LED modules, 1 green emission filter
1708285 ChemiDoc MP Blue LED Module Kit, for use with applications requiring blue fluorophore detection, 3,185.00
includes 2 epi-blue LED modules, 1 blue emission filter
1708294 ChemiDoc MP IQ/OQ, for use with Image Lab software 2,000.00
1708182 XcitaBlue Conversion Screen, includes view goggles; blue conversion screen for viewing SYBR Green, 1,250.00
SYBR Safe, GFP, Flamingo, and other fluorescent gel stains
1708183 XcitaBlue Conversion Screen and Filter, includes view goggles and SYBR Safe filter #1708075; 1,705.00
blue conversion screen for viewing SYBR Green, SYBR Safe, and other fluorescent gel stains
1708289 White Light Conversion Screen, for use with ChemiDoc MP, ChemiDoc XRS+, and Gel Doc XR+ systems 555.00
1706887 365 nm UV Lamps, 6 replacement bulbs 290.00
1708097 Standard 302 nm UV Lamps, 6 replacement bulbs 335.00
1708089 Mitsubishi Thermal Printer 1,715.00
1707581 Mitsubishi Thermal Printer Paper, 4 rolls, for use with Mitsubishi printer 155.00
1708184 Gel Alignment Templates, pkg of 3, templates for aligning gels and blots, for use with ChemiDoc XRS+, 48.00
ChemiDoc MP, and Gel Doc XR+ systems
Smart Imaging
Modular design — use specific trays for specific
■■
Ordering Information
Catalog # Description Price, U.S. $
1708270 el Doc EZ Imaging System with Image Lab Software, compatible with PC or Mac, includes darkroom,
G 7,725.00
camera, cables, Image Lab software; stain-free sample tray #1708274; other sample trays available separately
1708277 Gel Doc EZ IQ/OQ, for use with Image Lab software 1,575.00
Gel Doc EZ Sample Trays
1708271 UV Sample Tray, for gels using ultraviolet illumination 1,030.00
1708272 White Sample Tray, for gels stained with Coomassie Blue, copper, silver, or zinc stains 1,030.00
1708273 Blue Sample Tray, for gels stained with GelGreen or any SYBR stain 1,030.00
1708274 Stain-Free Sample Tray, for stain-free gels and blots 1,030.00
1708276 Sample Tray Holder, holds 4 sample trays 300.00
Accessories
1707581 Mitsubishi Thermal Printer Paper, 4 rolls, for use with Mitsubishi thermal printer 155.00
1708089 Mitsubishi Thermal Printer 1,715.00
1708097 Standard 302 nm UV Lamps, 6 replacement bulbs 335.00
ChemiDoc XRS+ System is now compatible with stain-free technology, which offers See Also
The ChemiDoc XRS+ system offers sensitive checkpoints in western blot experiments and a convenient Stain-free gels:
chemiluminescence detection in addition to gel and blot total protein loading control for data normalization. pages 168, 178.
mini, midi formats
documentation of fluorescent and colorimetric samples. For More Information V3 Western Workflow:
The system includes a sensitive 16-bit CCD camera that Web: bio-rad.com/imagingsystems page 221.
is cooled for detection of faint samples. The XRS+ system Request or download bulletin: 5837 Trans-Blot Turbo
transfer system:
page 224.
Ordering Information
Catalog # Description Price, U.S. $
1708195 el Doc XR+ System with Image Lab Software, compatible with PC or Mac, includes darkroom,
G Inquire
UV transilluminator, epi-white illumination standard filter, camera, cables, Image Lab software
1708193 Gel Doc XR+ IQ/OQ, for use with Image Lab software 2,170.00
1708265 ChemiDoc XRS+ System with Image Lab Software, compatible with PC or Mac, includes Inquire
darkroom, UV transilluminator, epi-white illumination standard filter, camera, power supply, cables,
Image Lab software
1708256 ChemiDoc XRS+ IQ/OQ, for use with Image Lab software 1,760.00
Accessories
1708199 Gel Doc XR+ Installation Kit 1,085.00
1708299 ChemiDoc XRS+ Installation Kit 905.00
1708289 White Light Conversion Screen, for use with ChemiDoc MP, ChemiDoc XRS+, and Gel Doc XR+ systems 555.00
1708074 Filter, 520DF30, 62 mm, for SYBR Green I/GFP/SYBR Gold/fluorescein stain 800.00
1708075 Filter, 560DF50, 62 mm, for Cy3/rhodamine stains 800.00
1708076 Filter, 630BP30, 62 mm, for SYPRO Ruby/Texas Red stains 800.00
1708081 Filter, standard emission, 62 mm 400.00
1706887 365 nm UV Lamps, 6 replacement lamps 290.00
1708097 Standard 302 nm UV Lamps, 6 replacement bulbs 335.00
1708089 Mitsubishi Thermal Printer 1,715.00
1707581 Mitsubishi Thermal Printer Paper, 4 rolls, for use with Mitsubishi thermal printer 155.00
1708183 XcitaBlue Conversion Screen Kit, includes viewing goggles and standard detection filter 1,705.00
1708008 Orange Fluorescence Reference Plate 120.00
1703759 Bio-Rad Fluorescent Ruler 21.00
1703760 Gel Cutter Ruler 21.00
1708184 Gel Alignment Templates, pkg of 3, templates for aligning gels and blots, for use with ChemiDoc XRS+, 48.00
ChemiDoc MP, and Gel Doc XR+ systems
1708026 Image Lab Focus Calibration Target 86.00
See Also PharosFX™ and Personal Molecular Imager™ (PMI™ ) Systems Accessories
Fluorescent protein
gel stains: page 201.
Nucleic acid stain: Accessories for the discontinued Pharos FX and PMI
page 259. systems are still available and include replacement filters,
Gel analysis software: external laser upgrades, sample trays, imaging screens,
page 293.
exposure cassettes, and screen erasers. For alternative
imaging solutions, please see the imager selection guide
(page 280) and ChemiDoc™ MP (page 282) for differential
gel electrophoresis needs.
For More Information
Web: bio-rad.com/pharos
Ordering Information
Catalog # Description Price, U.S. $
Accessories for PharosFX and PharosFX Plus Systems
1707896 Filter 640 nm BP, for Texas Red dye 260.00
1709459 Filter 530 nm BP, for ECL Plus, AttoPhos, SYBR Green I, Alexa Fluor 488, FITC, Cy2, and Pro-Q Emerald dyes 710.00
1707866 Filter 605 nm BP, for ethidium bromide, SYPRO Red, SYPRO Ruby, Alexa Fluor 532 and 546, and Cy3 dyes 500.00
1707865 Filter 695 nm BP, for Cy5 and Alexa Fluor 635 dyes 560.00
1707863 Filter 555 nm LP, for Texas Red dye 715.00
1707867 Blank Filter Holder 34.00
Accessories for PharosFX, PharosFX Plus, and PMI Systems
1707811 Sample Tray 685.00
1707812 Multi-Sample Tray I, for small aluminum-mounted screens and microplates 575.00
1707814 Microplate Adaptor, for multi-sample tray I 400.00
1707819 Multi-Sample Tray II, for scanning gels mounted to glass plates 695.00
Accessories for PharosFX Plus and PMI Systems
1707843 Imaging Screen-K (Kodak), 20 x 25 cm 1,600.00
1707841 Imaging Screen-K (Kodak), 35 x 43 cm 3,070.00
1707861 Exposure Cassette-K, for 20 x 25 cm Kodak screen 330.00
1707862 Exposure Cassette-K, for 35 x 43 cm Kodak screen 430.00
1707809 Eraser Screen-K, 110/120 V 2,715.00
1707806 Eraser Screen-K, 220/240 V 2,715.00
and blue CCD technology to optimally scan and Image Lab software:
page 293.
quantitate colorimetric blots and gels treated with a
variety of stains
■■
ccurate quantitation of samples over a large dynamic
A
range (up to 3.4 OD) ensuring detection of both highly
abundant and dilute proteins
■■
canning of larger gels for enhanced separation of
S
proteins on oversized 29 x 33 cm imaging area
■■
IQ/OQ kit available for validation of the calibration
functions using a NIST-traceable external target to
confirm the accuracy of the internal target, guaranteeing
accurate and reproducible results
■■
igh resolution and analysis of closest bands on a gel
H
due to 16-bit precision and 36.3 µm resolution
■■
ealed imaging area to accommodate wet samples of
S
variable thickness
■■
urity analysis and lane background tools for
P
manufacturing QC
■■
.S. FDA 21 CFR Part 11 regulation compliance
U
software available
For More Information
Web: bio-rad.com/gs900
Request or download bulletin: 6385
Ordering Information
Catalog # Description Price, U.S. $
1707991 S-900 Calibrated Densitometry System, PC-compatible calibrated densitometer, cables,
G Inquire
Image Lab software, Biologics Analysis Workflow Starter Kit (Criterion cell, Criterion TGX precast gels,
Precision Plus Protein standards, QC colloidal Coomassie stain, buffers)
1707993 GS-900 Regulatory Tools Package, includes GS-900 IQ/OQ Kit and Image Lab Security Edition 21 CFR 8,400.00
Part 11 module, 1 license
1707994 GS-900 IQ/OQ Kit, set of protocols for installation qualification/operational qualification and NIST-traceable 5,140.00
external step tablet for the GS-900 calibrated densitometer
Ordering Information
Catalog # Description Price, U.S. $
Accessories
1657202 Cutting Tip, 1.0 mm 245.00
1657203 Cutting Tip, 1.5 mm 245.00
1657204 Glass Bottle, 1 L 58.00
1657205 Calibration Pucks, 10 58.00
1657206 Membrane Cutting Head, with 1.0 mm tip 705.00
1657207 Membrane Cutting Tip, 1.0 mm 245.00
1657208 Gel Cutting Sheets, 15 96.00
1657209 Gel Holding Clips, 2 pair 295.00
1657210 Calibration Target 64.00
1657211 Camera Target 58.00
1657212 Micro Tubes, 1.5 ml, 20 82.00
1657214 Bottle Holder 215.00
1657215 Gel Tray 355.00
1657217 Round-Bottom Microplates, 96-well, 20 96.00
1657218 Ferrule, 10–32, 1/16" OD, 10 76.00
1657219 Barcode Reader 505.00
1709630 PDQuest Advanced 2-D Analysis Software, 1 user license, 2-D analysis software Inquire
provides advanced functionality
Subject Index
ChemiDoc™ MP Imaging System, 282
ChemiDoc™ Touch Imaging System, 281
EXQuest™ Spot Cutter Accessories, 288
Gel Doc™ EZ Imaging System, 283
Gel Doc™ XR+ and ChemiDoc™ XRS+ Systems, 284
GS-900™ USB Calibrated Densitometer, 287
PharosFX™ and Personal Molecular Imager™ (PMI™ )
Systems Accessories, 286
292 | Imaging and Analysis Software In the U.S., call toll free at 1-800-424-6723
Imaging and Analysis Software
bio-rad.com/bioinformatics
■■
erforms flat fielding corrections specifically and
P information organized lane by lane and band by band
consistently for every application each time a data analysis is performed or modified
■■
Generates accurate data and publication-quality images ■■
opies any part of a data table to popular document
C
Automated or Manual Data Analysis processing applications such as Adobe Acrobat,
Automatically performs all the image analysis steps;
■■ Microsoft Word, or Microsoft Excel
can be user-modified for more precise band detection, ■■
aves reports within customized protocols designed
S
control of background level, and lane selection by the user
■■
esults table is instantly updated when experimental
R ■■
rovides multiple tools for displaying, viewing,
P
parameters are changed and annotating images. Provides various file formats for
■■
ffers optional manual image analysis adjustments by
O exporting images for publications and presentations.
the user in every step For More Information
Web: bio-rad.com/imagelabsoftware
Ordering Information
Catalog # Description Price, U.S. $
1709690 Image Lab Software, compatible with PC or Mac, for automated image capture, optimization, and 1-D gel 1,085.00
or blot analysis, for use with Gel Doc EZ, Gel Doc XR+, ChemiDoc XRS+, ChemiDoc Touch, ChemiDoc MP,
and GS-900 systems
1709691 Image Lab Software, Security Edition for 21 CFR Part 11 compliance, 1 license 3,705.00
1709692 Image Lab Software, Security Edition for 21 CFR Part 11 compliance, 5 licenses Inquire
1709693 Image Lab Software, Security Edition for 21 CFR Part 11 compliance, 10 licenses Inquire
In the U.S., call toll free at 1-800-424-6723 Imaging and Analysis Software | 293
Imaging and Analysis Software
bio-rad.com/bioinformatics
294 | Imaging and Analysis Software In the U.S., call toll free at 1-800-424-6723
Imaging and Analysis Software
bio-rad.com/bioinformatics
■■
Comparative analysis ■■
Options for network licenses
■■
Biological relationship models For More Information
Web: bio-rad.com/pdquest
■■
Comprehensive reports
Request or download bulletin: 3121
Automation
Repeatable analysis for samples of similar types
■■
■■
Recallable templates
■■
Batch processing of multiple experiments
Information Repository
■■
Flexible annotation features
■■
ny type of characterizing data can be linked to
A
each spot on a master gel image
■■
asy to view and share information associated with
E
identified proteins
Ordering Information
Catalog # Description Price, U.S. $
1709630 PDQuest Advanced 2-D Analysis Software Inquire
1709631 PDQuest Advanced 1-User Network License Inquire
1709632 PDQuest Advanced 2-User Network License Inquire
1709633 PDQuest Advanced 3-User Network License Inquire
1709635 PDQuest Advanced 5-User Network License Inquire
1709636 PDQuest Advanced 10-User Network License Inquire
1709640 PDQuest Basic to Advanced Software Version Upgrade Inquire
1709645 PDQuest Advanced CFR Module 8,110.00
1709620 PDQuest Basic 2-D Analysis Software 9,460.00
1709660 PDQuest Basic Software Version Upgrade, 7.x–8.0 4,225.00
1709670 PDQuest Advanced Software Version Upgrade, 7.x–8.0 Inquire
In the U.S., call toll free at 1-800-424-6723 Imaging and Analysis Software | 295
Imaging and Analysis Software
Subject Index
Subject Index
Image Lab™ Software, 293
PDQuest™ 2-D Analysis Software, Version 8.0, 294
296 | Imaging and Analysis Software In the U.S., call toll free at 1-800-424-6723
Bio-Plex® Multiplex System
Bio-Plex® Multiplex System 298
Instruments, Software, and Tools 298
The Bio-Plex multiplex platform is built on the most flexible technology available. Bio-Rad offers instruments to satisfy a
variety of research needs and budgets for multiplex applications. These readers include the versatile Bio-Plex 200 system
and the high-throughput capacity Bio-Plex 3D suspension array system.
Learn More about the Technology
Web: bio-rad.com/tech/bio-plex
298 | Bio-Plex Multiplex System In the U.S., call toll free at 1-800-424-6723
Bio-Plex Multiplex System
bio-rad.com/bio-plex Instruments, Software, and Tools
Ordering Information
Catalog # Description Price, U.S. $
171000201 io-Plex 200 System, 100–240 V, includes array reader, microplate platform, Bio-Plex Manager software
B Inquire
with workstation license, PC, monitor, calibration kit, validation kit 4.0, MCV plate IV, Bio-Plex reservoir,
20 L sheath fluid, spare sample needle
171000205 Bio-Plex 200 System with HTF, same as #171000201 with high-throughput fluidics (HTF) Inquire
Bio-Plex 200 Accessories and Replacement Parts
171203050 Bio-Plex Reservoir 82.00
171000055 Sheath Fluid, 1x, 20 L 69.00
171002002 Communication Cable, 3' CAN BUS cable, connects the array reader with the HTF system 305.00
171002003 Communication Cable, 5' USB cable, connects the array reader with a PC USB port 320.00
171002010 Sheath Fluid Bottle, 1 L 420.00
171002012 Sheath Waste Bottle, 1 L 420.00
171002020 Sample Needle, 4.6" (11.6 cm) 310.00
171002024 Alignment Guide, aligns the array reader on the microplate platform 250.00
171002026 Needle Adjustment Wrench 98.00
171002030 Protective Shield for Sample Needle 135.00
171002032 Air Intake Filter 235.00
171002033 Syringe Seal 92.00
171002034 Syringe Seal with Cylinder 435.00
171002038 Sheath Fluid Filter with Quick Connect Tubing, removes particles >5 µm in diameter 275.00
171002040 Sheath Cube Filter, pkg of 1, air tubing HTF to reader, sheath tubing HTF to reader, tubing sheath container 230.00
to HTF (including 10 µm filter), and waste tubing (reader to waste container)
171002056 HTF Tubing, pkg of 1, air tubing HTF to reader, sheath tubing HTF to reader, tubing sheath container to HTF 490.00
(including 10 µm filter), waste tubing (reader to waste container)
171002023 Needle Guide, allows sample needle access to microplates 185.00
Bio-Plex 200 System Performance Validation and Calibration Tools
171203001 Bio-Plex Validation Kit 4.0, includes optics validation, reporter validation, classify validation, and 1,765.00
fluidics validation bead sets for approximately 50 validation routines using Bio-Plex Manager software,
current version (Standard or Security Edition), and MCV plate IV
171203033 Bio-Plex MCV Plate IV, for use with Bio-Plex Manager software, current version 180.00
(Standard or Security Edition), and validation kit 4.0
171203060 Bio-Plex Calibration Kit, includes Cal1 and Cal2 calibration beads for approximately 50 daily calibration routines 580.00
In the U.S., call toll free at 1-800-424-6723 Bio-Plex Multiplex System | 299
Bio-Plex Multiplex System
Instruments, Software, and Tools bio-rad.com/bio-plex
Ordering Information
Catalog # Description Price, U.S. $
BioPlex3D io-Plex 3D Multiplex System, includes Bio-Plex 3D multiplex system, xPONENT acquisition software, PC,
B Inquire
calibration and verification reagents, Bio-Plex Manager desktop license
892018500001* xPONENT 21 CFR Part 11 Software Module, enables U.S. FDA 21 CFR Part 11 compliance Inquire
892018700001* xPONENT Automation Module, enables interfacing with robotics workstations 2,745.00
171022001* Swivel Base, allows rotation of the instrument away from the robotics workstation for maintenance and Inquire
sample loading
892018600001* xPONENT LIS Software Module, enables interfacing with LIMS/LIS databases 1,110.00
892018200001* xPONENT Extra Seat Licenses, 3 additional seats of xPONENT software 1,110.00
FM3D-CAL-K25 Bio-Plex 3D Calibration Kit, good for 25 uses; includes reagents, CD, 25 stripwells 900.00
FM3D-PVER-K25 Bio-Plex 3D Performance Verification Kit, good for 25 uses; kit includes reagents, fluidics, CD, 25 stripwells 1,040.00
* All additional accessories may be purchased through Bio-Rad Laboratories, Inc. at the time of system purchase.
Post–system purchase accessories must be purchased directly from Luminex Corporation.
300 | Bio-Plex Multiplex System In the U.S., call toll free at 1-800-424-6723
Bio-Plex Multiplex System
bio-rad.com/bio-plex Instruments, Software, and Tools
Ordering Information
Catalog # Description Price, U.S. $
Kits and Reagents
171213003 Bio-Plex MAGPIX Drive Fluid, 4 x 700 ml drive fluid for use with Bio-Plex MAGPIX multiplex reader 75.00
171213001 Bio-Plex MAGPIX Calibration Kit, calibration kit good for 25 uses; kit includes 5 ml calibrator, 540.00
microspheres, CD, pkg of 25 8-well strips
171213002 Bio-Plex MAGPIX Verification Kit, verification kit good for 25 uses; kit includes MAGPIX verifier, 5 ml of 605.00
microspheres, MAGPIX fluidics 1, MAGPIX fluidics 2, MAGPIX performance verification kit CD, pkg of 25
8-well strips
Bio-Plex MAGPIX Software
171051555 Bio-Plex Manager MP Software Upgrade, includes Bio-Plex Manager MP software, getting started guide, 7,430.00
probe height adjustment plate, upgrade quick guide, and Bio-Plex Manager 6.1
Bio-Plex MAGPIX Accessories
171012004 Bio-Plex MAGPIX Replacement Waste Fluid Container, pkg of 1, 850 ml container, holds waste fluid, 53.00
for use with Bio-Plex MAGPIX reader
171012005 Bio-Plex MAGPIX Sample Probe, sample probe needle for use with Bio-Plex MAGPIX multiplex reader 210.00
171012006 Bio-Plex MAGPIX Sample Probe Height Adjustment Kit, sample probe height adjustment kit for use 105.00
with Bio-Plex MAGPIX multiplex reader
171012008 Bio-Plex MAGPIX 96-Well Plate Heater Block, 96-well plate heater block for use with Bio-Plex MAGPIX 435.00
multiplex reader
30034376 Bio-Plex Pro Wash Station, for magnetic bead–based assays, includes magnetic plate carrier, Inquire
waste bottle, 2 liquid bottles
171020100 Bio-Plex Handheld Magnetic Washer, includes magnetic washer and adjustment hex tools for use in 760.00
manual wash steps for all Bio-Plex magnetic assays
171061000 Bio-Plex Probe Height Adjustment Plate, replacement plate for Bio-Plex MAGPIX multiplex reader 705.00
In the U.S., call toll free at 1-800-424-6723 Bio-Plex Multiplex System | 301
Bio-Plex Multiplex System
Instruments, Software, and Tools bio-rad.com/bio-plex
Ordering Information
Catalog # Description Price, U.S. $
30034376 io-Plex Pro Wash Station, includes magnetic plate carrier, waste bottle, 2 buffer bottles for use with
B Inquire
magnetic beads
171020100 Bio-Plex Handheld Magnetic Washer, includes magnetic washer and adjustment hex tools for washing all 760.00
Bio-Plex magnetic assays
Accessories
171025001 io-Plex Pro Flat Bottom Plates, 40 x 96-well plates, for washing magnetic beads using the Bio-Plex Pro
B 240.00
or Bio-Plex Pro II wash stations
171304500 Bio-Plex Wash Buffer, 1.5 L 96.00
171304502 Filter Plate, 1 x 96-well filter plate with clear plastic lid, plate holder tray, for washing nonmagnetic beads 27.00
using the Bio-Plex Pro II wash station, optional vacuum manifold; sealing tape not included
Bio-Plex Manager software is a comprehensive, all-in-one Data Export, Cross-Platform Capabilities, and
software package that provides advanced data analysis for System Compatibility
multiplex assays. Import and analyze data from xPONENT and other
■■
third-party software
Simple Operation
■■
Ability to self train — just follow the steps outlined on
■■
se any commercial or custom xMAP assay built on
U
the screen any Luminex microsphere
■■
amiliar Windows interface — no new terms or menus
F
■■
opy and paste data into popular word processing
C
to learn and presentation software applications
■■
Export to text, XML, and Excel in column or 96-well format
Save Time
Simplified input — values for standards are easy to enter;
■■
■■
nalyze data in LIMS and other third-party software by
A
automatic addition of new lots reduces setup time customizing export options
■■
utomated data processing — proprietary algorithm
A
■■
equest Bio-Plex Manager 4.1.1 software for
R
automatically sets the appropriate dynamic range and installation on Luminex systems with IS 2.3 software
identifies data points outside this range
Bio-Plex Manager Software Licenses
■■
Immediate recalculation of results efficiently updates data
when settings are changed License Capabilities and Uses
Instrument control Full-system, data acquisition and analysis; included
Use Tools That Meet Your Unique Needs with all Bio-Plex 200 systems
Superior sensitivity and dynamic range with onboard
■■
Desktop Single-user license for protocol setup and data
Brendan Scientific StatLIA 4PL and 5PL weighted analysis; does not control the array reader
curve-fitting models Network Multiuser (5, 10, 25, or 50) licenses for data access
from a server; does not control the array reader
■■
Data normalization using multiple internal controls
Security Edition Enables U.S. FDA 21 CFR Part 11 compliance
(housekeeping genes) for gene expression applications
■■
ene Manager analysis module for presence and absence
G
analysis, pathogen detection, and genotyping applications System Requirements
CPU Pentium 4 or higher, PC only
Analyze Data with Easy-to-Use Reports Operating system Windows XP Professional, Windows 7
Automated export of standard curve graphs
■■
■■
View of all wells or summary (average) of replicate samples
For More Information
■■
Data organized by sample type, group, or well Web: bio-rad.com/bio-plexmanager
Request or download bulletin: 5613
■■
One-click navigation between analytes
■■
Flexible data presentation
302 | Bio-Plex Multiplex System In the U.S., call toll free at 1-800-424-6723
Bio-Plex Multiplex System
bio-rad.com/bio-plex Instruments, Software, and Tools
Ordering Information
Catalog # Description Price, U.S. $
171STND01 io-Plex Manager Software, 1-user desktop license, to analyze Bio-Plex data and generate protocols,
B Inquire
does not operate the instrument
171STND05 Bio-Plex Manager Software, 5-user desktop license to analyze Bio-Plex data and generate protocols, Inquire
does not operate the instrument
171STND10 Bio-Plex Manager Software, 10-user desktop license to analyze Bio-Plex data and generate protocols, Inquire
does not operate the instrument
171STND25 Bio-Plex Manager Software, 25-user desktop license to analyze Bio-Plex data and generate protocols, Inquire
does not operate the instrument
Contact Bio-Rad Technical Support to verify whether your current system is compatible with these software products.
Ordering Information
Catalog # Description Price, U.S. $
171STND23 io-Plex Manager Software for IS 2.3 System, system license, most current version, for converting Luminex IS
B Inquire
and xPONENT systems
171SUPG30 Bio-Plex Manager Software for 3.0 Instrument Control Software, system license for upgrading standard or 4,875.00
security software version 3.0 to current Bio-Plex Manager version (Security Edition upgrade requires #171SCRT00)
171SUPG40 Bio-Plex Manager Software for 4.0 Instrument Control Software, system license for upgrading the standard or 1,305.00
security software version 4.0 to current Bio-Plex Manager version (Security Edition upgrade requires #171SCRT00)
171SUPG41 Bio-Plex Manager Software for 4.1 Instrument Control Software, system license for upgrading the standard or 870.00
security software version 4.1 to current Bio-Plex Manager version (Security Edition upgrade requires #171SCRT00)
171SUPG50 Bio-Plex Manager Software for 5.0 Instrument Control Software, system license for upgrading the standard or 870.00
security software version 5.0 to current Bio-Plex Manager version (Security Edition upgrade requires #171SCRT00)
In the U.S., call toll free at 1-800-424-6723 Bio-Plex Multiplex System | 303
Bio-Plex Multiplex System
Instruments, Software, and Tools bio-rad.com/bio-plex
Ordering Information
Catalog # Description Price, U.S. $
171SCRT00 io-Plex Manager Instrument Control License, Security Edition, upgrades workstation with
B Inquire
Bio-Plex Manager software, allows compliance with U.S. FDA 21 CFR Part 11 regulations
171SCRT01 Bio-Plex Manager Software, Security Edition desktop, includes 1-user desktop license, Inquire
allows compliance with U.S. FDA 21 CFR Part 11 regulations. Does not operate the instrument.
171SCRT05 Bio-Plex Manager Software, Security Edition desktop, includes 5-user network license, Inquire
allows compliance with U.S. FDA 21 CFR Part 11 regulations. Does not operate the instrument.
Contact Bio-Rad Technical Support to verify whether your current system is compatible with these software products.
Ordering Information
Catalog # Description Price, U.S. $
171001523 io-Plex Data Pro Plus Software, includes 5 Bio-Plex Data Pro Plus software licenses
B 6,170.00
171001513 Bio-Plex Data Pro Software, includes 5 Bio-Plex Data Pro software licenses 5,415.00
304 | Bio-Plex Multiplex System In the U.S., call toll free at 1-800-424-6723
Bio-Plex Assays
bio-rad.com/bio-plex Bio-Plex Pro Human MMP and TIMP Assays
Bio-Plex Assays ®
The Bio-Plex Pro assays for human matrix Product features include:
metalloproteinases (MMPs) and tissue inhibitors of ■■
ingle dilution factor protocol, measure 9 MMPs
S
matrix metalloproteinases (TIMPs) consist of panels with less work
of 9 MMPs and 4 TIMPs. ■■
Magnetic beads for simplified plate processing
MMPs degrade extracellular matrix proteins, whereas
■■
Flexible ordering options — order either
TIMPs work to inhibit the activity of MMPs. Studying MMPs premixed all-in-one kits or singleplex sets,
and TIMPs together can reveal disruptions in the balance or customize your assay with the Bio-Plex assay
of activity between these two protein families, providing builder (bio-rad.com/assaybuilder)
insights into complex disease states.
■■
Single-level quality controls with lot-specific ranges
■■
Assay quick guide to get you started right away
These immunoassays are built on magnetic beads to ■■
High-quality design for robust and reproducible
enable robust quantification of multiple proteins in human measurements
samples for research involving the following conditions and
For More Information
disease pathways: Web: bio-rad.com/bio-plexmmpsandtimps
Request or download bulletin: 6551
Associated Research Associated Pathways
Cancer Inflammation Analytes in the MMP Panel Analytes in the TIMP Panel
Cardiovascular disease Metastasis MMP-1 TIMP-1
Infectious disease Apoptosis MMP-2 TIMP-2
Autoimmune disease Angiogenesis MMP-3 TIMP-3
Neurology Tumor invasion MMP-7 TIMP-4
Wound healing MMP-8
MMP-9
MMP-10
MMP-12
MMP-13
Ordering Information
Catalog # Description Price, U.S. $
BioPlex Pro Human MMP and TIMP Premixed All-in-One Kits
171AM001M Bio-Plex Pro Human MMP Panel 9-Plex, 1 x 96-well, includes coupled magnetic capture beads, premixed 2,180.00
detection antibodies, standards, quality controls, detection antibody diluent HB, standard diluent HB, sample diluent
HB, assay buffer, wash buffer, streptavidin-PE, 96-well flat bottom plate, sealing tape, and instructions, for the
detection of MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-12, and MMP-13
171AM002M Bio-Plex Pro Human TIMP Panel 4-Plex, 1 x 96-well, includes coupled magnetic capture beads, premixed 1,220.00
detection antibodies, standards, quality controls, detection antibody diluent HB, diluent HD, assay buffer, wash
buffer, streptavidin-PE, 96-well flat bottom plate, sealing tape, and instructions, for the detection of TIMP-1, TIMP-2,
TIMP-3, and TIMP-4
Bio-Plex Pro MMP Singleplex Sets
171BM001M MMP-1 Set 270.00 171BM006M MMP-9 Set 270.00
171BM002M MMP-2 Set 270.00 171BM007M MMP-10 Set 270.00
171BM003M MMP-3 Set 270.00 171BM008M MMP-12 Set 270.00
171BM004M MMP-7 Set 270.00 171BM009M MMP-13 Set 270.00
171BM005M MMP-8 Set 270.00
Standards
171DM0001 MMP Standard, 1 pkg of 1 vial, lyophilized mixture of 9 standard analytes: MMP-1, MMP-2, MMP-3, MMP-7, 190.00
MMP-8, MMP-9, MMP-10, MMP-12, and MMP-13
171DM0501 MMP Standard, pkg of 50 lot-matched vials, lyophilized mixture of 9 standard analytes: MMP-1, MMP-2, 7,520.00
MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-12, and MMP-13
In the U.S., call toll free at 1-800-424-6723 Bio-Plex Multiplex System | 305
Bio-Plex Assays
Bio-Plex Pro RBM Human Metabolic and Hormone Panels bio-rad.com/bio-plex
The Bio-Plex Pro RBM human metabolic and hormone Key features include:
panels, developed in partnership with Myriad RBM®, ■■
nalytically validated to standards set forth by the
A
comprise a highly relevant set of biomarkers involved in Clinical Laboratory Standards Institute (CLSI)
diabetes, obesity, metabolic syndrome, cardiovascular ■■
Manufactured in accordance with GMP guidelines
disease, and hormonal control of metabolism and
reproductive organs.
■■
ot-to-lot correlation specification of R2 ≥ 0.9 for
L
consistently reproducible results
The assays are built on magnetic beads to enable robust ■■
2-level quality controls with lot-specific ranges
quantification of multiple proteins in human serum, plasma,
and cell culture media samples. Assays are offered as
■■
Assay quick guide to get you started right away
premixed all-in-one kits for research involving: ■■
astest available assay protocols and a convenient
F
■■
Gut hormones and adipokines all-in-one kit format
■■
Pituitary hormones ■■
Magnetic beads for simplified plate processing
■■
Diabetes, type I and type II For More Information
■■
Metabolic syndrome Web: bio-rad.com/bio-plexmetabolic
■■
Obesity Request or download bulletin: 6571
■■
Cardiovascular disease
■■
Inflammation
The Bio-Plex Pro RBM human metabolic and hormone assays are available in 7 panels:
Human Human Human Human Human Human Human
Metabolic Metabolic Metabolic Metabolic Hormone IGFBP IGF
Panel 1 Panel 2 Panel 3 Panel 4 Panel 1 Panel Panel
C-Peptide FGF-21 AAT ANGPTL3 FSH IGFBP-1 IGF-1
Cortisol FGF-23 AGP-1 Chemerin GH IGFBP-2 IGF-2
Pancreatic
Galectin-3 Hemopexin DPP4 LH IGFBP-3
polypeptide
Proinsulin sLeptin R RBP-4 Protein S Prolactin IGFBP-4
Peptide YY Omentin-1 Transferrin SEPP TSH IGFBP-5
Pentraxin-3 Transthyretin IGFBP-6
PON-1 VDBP IGFBP-7
Vaspin
Ordering Information
Catalog # Description Price, U.S. $
Human Metabolic Premixed All-in-One Kits
171AMR1CK Bio-Plex Pro RBM Human Metabolic Panel 1, 1 x 96-well, includes premixed magnetic capture beads and 1,435.00
detection antibodies, standards, 2-level controls, standard diluent, buffers (blocking, sample dilution, and 10x
assay), 10x streptavidin-PE, flat bottom plate/seals, and instructions, for the detection of C-Peptide, cortisol,
pancreatic polypeptide, proinsulin, and peptide YY
171AMR2CK Bio-Plex Pro RBM Human Metabolic Panel 2, 1 x 96-well, includes premixed magnetic capture beads and 2,070.00
detection antibodies, standards, 2-level controls, standard diluent, buffers (blocking, sample dilution, and 10x
assay), 10x streptavidin-PE, flat bottom plate/seals, and instructions, for the detection of FGF-21, FGF-23,
galectin-3, sLeptin R, omentin-1, pentraxin-3, PON-1, and vaspin
171AMR3CK Bio-Plex Pro RBM Human Metabolic Panel 3, 1 x 96-well, includes premixed magnetic capture beads 1,900.00
and detection antibodies, standards, 2-level controls, standard diluent, buffers (blocking, sample dilution,
and 10x assay), 10x streptavidin-PE, flat bottom plate/seals, and instructions, for the detection of AAT, AGP-1,
hemopexin, RBP-4, transferrin, transthyretin, and VDBP
continues
306 | Bio-Plex Multiplex System In the U.S., call toll free at 1-800-424-6723
Bio-Plex Assays
bio-rad.com/bio-plex Bio-Plex Pro Human Inflammation Assays
Ordering Information
Catalog # Description Price, U.S. $
171AMR4CK Bio-Plex Pro RBM Human Metabolic Panel 4, 1 x 96-well, includes premixed magnetic capture beads and 1,540.00
detection antibodies, standards, 2-level controls, standard diluent, buffers (blocking, sample dilution, and 10x
assay), 10x streptavidin-PE, flat bottom plate/seals, and instructions, for the detection of ANGPTL3, chemerin,
DPP4, protein S, and SEPP
171AHR1CK Bio-Plex Pro RBM Human Hormone Panel 1, 1 x 96-well, includes premixed magnetic capture beads and 1,380.00
detection antibodies, standards, 2-level controls, standard diluent, buffers (blocking, sample dilution, and
10x assay), 10x streptavidin-PE, flat bottom plate/seals, and instructions, for the detection of FSH, GH, LH,
prolactin, and TSH
171AGR1CK Bio-Plex Pro RBM Human IGFBP Panel, 1 x 96-well, includes premixed magnetic capture beads and 1,650.00
detection antibodies, standards, 2-level controls, standard diluent, buffers (blocking, sample dilution, and
10x assay), 10x streptavidin-PE, flat bottom plate/seals, and instructions, for the detection of IGFBP-1,
IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, IGFBP-6, and IGFBP-7
171AFR1CK Bio-Plex Pro RBM Human IGF panel, 1 x 96-well, includes premixed magnetic capture beads and detection 810.00
antibodies, standards, 2-level controls, standard diluent, buffers (blocking, sample dilution, and 10x assay),
10x streptavidin-PE, flat bottom plate/seals, and instructions, for the detection of IGF-1 and IGF-2
The Bio-Plex Pro human inflammation assays consist of Key features include:
37 assays for biomarkers associated with inflammation ■■
Reproducibility — validated to meet analytical standards
pathways and consist of a 37-plex, 24-plex, and Treg ■■
Reliability — lot-to-lot calibration with low variability
cytokine 12-plex assay and 37 singleplex sets that can be
combined for a custom multiplex kit. ■■
Speed to results — Up to 1,480 data points in 4 hours
■■
lexible ordering options — order either premixed
F
The assays are built on magnetic beads to enable robust
all-in-one kits or singleplex sets, or customize
quantification of multiple proteins in human serum, plasma,
your assay with the Bio-Plex assay builder
and cell culture media samples. These assays are perfect
(bio-rad.com/assaybuilder)
for broad research applications, including academic and
preclinical studies in: For More Information
Web: bio-rad.com/bio-plexhumaninflammation
■■
Inflammation Request or download bulletin: 6625
■■
Autoimmune diseases
■■
Cancer research
The Bio-Plex Pro human inflammation panel includes the following targets:
APRIL / TNFSF13 sIL-6Ra IL-27 (p28) Osteocalcin
BAFF / TNFSF13B IL-8 IL-28A / IFN-l2 Osteopontin
sCD30 / TNFRSF8 IL-10 IL-29 / IFN-l1 Pentraxin-3
sCD163 Set IL-11 IL-32 sTNFR-1
Chitinase 3-like 1 IL-12 (p40) IL-34 sTNFR-2
gp130 / sIL-6Rb IL-12 (p70) IL-35 TSLP
IFN-a2 IL-19 LIGHT / TNFSF14 TWEAK / TNFSF12
IFN-b IL-20 MMP-1
IFN-g IL-22 MMP-2
IL-2 IL-26 MMP-3
In the U.S., call toll free at 1-800-424-6723 Bio-Plex Multiplex System | 307
Bio-Plex Assays
Bio-Plex Pro Human Inflammation Assays bio-rad.com/bio-plex
Ordering Information
Catalog # Description Price, U.S. $
BioPlex Pro Human Inflammation Premixed All-in-One Kits
171AL001M Bio-Plex Pro Human Inflammation Panel 1, 37-Plex, 1 x 96-well, includes coupled magnetic beads, detection 5,185.00
antibodies, standards, control, buffers, diluents, streptavidin-PE, 96-well plate, and sealing tape, for the detection
of 37 human inflammation biomarkers
171AL002M Bio-Plex Pro Human Inflammation Panel 2, 24-Plex, 1 x 96-well, includes coupled magnetic beads, detection 3,710.00
antibodies, standards, control, buffers, diluents, streptavidin-PE, 96-well plate, and sealing tape, for the detection
of 24 human inflammation biomarkers
171AL003M Bio-Plex Pro Human Inflammation Treg Cytokine Panel, 1 x 96-well, includes coupled magnetic beads, 2,225.00
detection antibodies, standards, control, buffers, diluents, streptavidin-PE, 96-well plate, and sealing tape,
for the detection of 12 human Treg biomarkers
Bio-Plex Pro Human Inflammation Singleplex Sets
171BL001M APRIL / TNFSF13 Set 215.00 171BL021M IL-27 (p28) Set 215.00
171BL002M BAFF / TNFSF13B Set 215.00 171BL022M IL-28A / IFN-l2 Set 215.00
171BL003M sCD30 / TNFRSF8 Set 215.00 171BL023M IL-29 / IFN-l1 Set 215.00
171BL004M sCD163 Set 215.00 171BL024M IL-32 Set 215.00
171BL005M Chitinase 3-like 1 Set 215.00 171BL025M IL-34 Set 215.00
171BL006M gp130 / sIL-6Rb Set 215.00 171BL026M IL-35 Set 215.00
171BL007M IFN-a2 Set 215.00 171BL027M LIGHT / TNFSF14 Set 215.00
171BL008M IFN-b Set 215.00 171BL028M MMP-1 Set 215.00
171BL009M IFN-g Set 215.00 171BL029M MMP-2 Set 215.00
171BL010M IL-2 Set 215.00 171BL030M MMP-3 Set 215.00
171BL011M sIL-6Ra Set 215.00 171BL031M Osteocalcin Set 215.00
171BL012M IL-8 Set 215.00 171BL032M Osteopontin Set 215.00
171BL013M IL-10 Set 215.00 171BL033M Pentraxin-3 Set 215.00
171BL014M IL-11 Set 215.00 171BL034M sTNFR-1 Set 215.00
171BL015M IL-12 (p40) Set 215.00 171BL035M sTNFR-2 Set 215.00
171BL016M IL-12 (p70) Set 215.00 171BL036M TSLP Set 215.00
171BL017M IL-19 Set 215.00 171BL037M TWEAK / TNFSF12 Set 215.00
171BL018M IL-20 Set 215.00 171DL0001 Std, 1PK 310.00
171BL019M IL-22 Set 215.00 171DL0050 Std, 50PK Inquire
171BL020M IL-26 Set 215.00
Standards
171304090M io-Plex Pro Reagent Kit III Flat Bottom Plate, 1 x 96-well, includes detection antibody diluent HB,
B 385.00
standard diluent HB, sample diluent HB, assay buffer, concentrated wash buffer, streptavidin-PE,
flat bottom plate, sealing tape, for magnetic separation methods
171DL0001 Bio-Plex Pro Human Inflammation Panel 1, 37-Plex Standard, pkg of 1 vial lyophilized mixture of 310.00
37 standard analytes
171DL0050 Bio-Plex Pro Human Inflammation Panel 1, 37-Plex Standard, pkg of 50 lot-matched vials, Inquire
lyophilized mixture of 37 standard analytes
308 | Bio-Plex Multiplex System In the U.S., call toll free at 1-800-424-6723
Bio-Plex Assays
bio-rad.com/bio-plex Bio-Plex Pro Cytokine, Chemokine, and Growth Factor Assays
Human Assays
In the U.S., call toll free at 1-800-424-6723 Bio-Plex Multiplex System | 309
Bio-Plex Assays
Bio-Plex Pro Cytokine, Chemokine, and Growth Factor Assays bio-rad.com/bio-plex
Ordering Information
Catalog # Description Price, U.S. $
Human Cytokine Premixed All-in-One Kits*
M50000007A Bio-Plex Pro Human Cytokine 8-Plex Panel, 1 x 96-well, includes coupled magnetic beads, detection antibodies, 1,605.00
standards, assay buffer, wash buffer, detection antibody diluent, streptavidin-PE, flat bottom plate, sealing tape,
standard diluent, sample diluent, for the detection of IL-2, IL-4, IL-6, IL-8, IL-10, GM-CSF, IFN-g, TNF-a
M5000031YV Bio-Plex Pro Human Cytokine 17-Plex Panel, 1 x 96-well, includes coupled magnetic beads, detection 2,710.00
antibodies, standards, assay buffer, wash buffer, detection antibody diluent, streptavidin-PE, flat bottom plate,
sealing tape, standard diluent, sample diluent, for the detection of IL-1b, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10,
IL-12 (p70), IL-13, IL-17, G-CSF, GM-CSF, IFN-g, MCP-1, MIP-1b, TNF-a
MF0005KMII Bio-Plex Pro Human Cytokine 21-Plex Panel, 1 x 96-well, includes coupled magnetic beads, detection 3,190.00
antibodies, standards, assay buffer, wash buffer, detection antibody diluent, streptavidin-PE, flat bottom plate,
sealing tape, standard diluent, sample diluent, for the detection of IL-1a, IL-2Ra, IL-3, IL-12 (p40), IL-16, IL-18, CTACK,
GRO-a, HGF, IFN-a2, LIF, MCP-3, M-CSF, MIF, MIG, b-NGF, SCF, SCGF-b, SDF-1a, TNF-b, TRAIL
M500KCAF0Y Bio-Plex Pro Human Cytokine 27-Plex Panel, 1 x 96-well, includes coupled magnetic beads, detection 4,085.00
antibodies, standards, assay buffer, wash buffer, detection antibody diluent, streptavidin-PE, flat bottom plate,
sealing tape, standard diluent, sample diluent, for the detection of IL-1b, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9,
IL-10, IL-12 (p70), IL-13, IL-15, IL-17, basic FGF, eotaxin, G-CSF, GM-CSF, IFN-g, IP-10, MCP-1, MIP-1a, MIP-1b,
PDGF-BB, RANTES, TNF-a, VEGF
M5000005L3 Bio-Plex Pro Human Cytokine Th1/Th2 Panel, 1 x 96-well, includes coupled magnetic beads, detection 1,820.00
antibodies, standards, assay buffer, wash buffer, detection antibody diluent, streptavidin-PE, flat bottom plate,
sealing tape, standard diluent, sample diluent, for the detection of IL-2, IL-4, IL-5, IL-10, IL-12 (p70), IL-13, GM-CSF,
IFN-g, TNF-a
Bio-Plex Pro Human Cytokine Singleplex Sets, Group I**, 1 x 96-Well
171B5001M IL-1b Set 215.00 171B5026M TNF-a Set 215.00
171B5002M IL-1ra Set 215.00 171B5027M VEGF Set 215.00
171B5003M IL-2 Set 215.00 171B6001M IL-1a Set 215.00
171B5004M lL-4 Set 215.00 171B6002M IL-2Ra Set 215.00
171B5005M IL-5 Set 215.00 171B6003M IL-3 Set 215.00
171B5006M lL-6 Set 215.00 171B6004M IL-12 (p40) Set 215.00
171B5007M IL-7 Set 215.00 171B6005M IL-16 Set 215.00
171B5008M IL-8 Set 215.00 171B6006M CTACK Set 215.00
171B5009M IL-9 Set 215.00 171B6007M GRO-a Set 215.00
171B5010M IL-10 Set 215.00 171B6008M HGF Set 215.00
171B5011M IL-12 (p70) Set 215.00 171B6009M ICAM-1 Set 215.00
171B5012M IL-13 Set 215.00 171B6010M IFN-a2 Set 215.00
171B5013M IL-15 Set 215.00 171B6011M LIF Set 215.00
171B5014M IL-17A Set 215.00 171B6012M MCP-3 Set 215.00
171B5015M Eotaxin Set 215.00 171B6013M M-CSF Set 215.00
171B5016M Basic FGF Set 215.00 171B6014M MIF Set 215.00
171B5017M G-CSF Set 215.00 171B6015M MIG Set 215.00
171B5018M GM-CSF Set 215.00 171B6016M b-NGF Set 215.00
171B5019M IFN-g Set 215.00 171B6017M SCF Set 215.00
171B5020M IP-10 Set 215.00 171B6018M SCGF-b Set 215.00
171B5021M MCP-1 Set 215.00 171B6019M SDF-1a Set 215.00
171B5022M MIP-1a Set 215.00 171B6020M TNF-b Set 215.00
171B5023M MIP-1b Set 215.00 171B6021M TRAIL Set 215.00
171B5024M PDGF-BB Set 215.00 171B6022M VCAM-1 Set 215.00
171B5025M RANTES Set 215.00
Note: IL-18 is available only in multiplex format.
Standards
171D50001 Bio-Plex Pro Human Cytokine Standards, pkg of 1 vial, lyophilized mixture of 48 analytes 185.00
171D10501 Bio-Plex Pro Human Cytokine Standards, pkg of 50 lot-matched vials, lyophilized mixture of 48 analytes 7,815.00
Reagent Kit — for use with human cytokine singleplex sets
171304070M Bio-Plex Pro Reagent Kit with Flat Bottom Plate, 1 x 96-well, includes flat bottom plate, assay buffer, 275.00
wash buffer, detection antibody diluent, streptavidin-PE, sealing tape, standard diluent, sample diluent,
and instructions, for magnetic separation methods
* Please contact your local Bio-Rad Sales Representative for pricing and availability of 10 x 96-well assay kits.
** Includes coupled magnetic beads and detection antibody. Requires the appropriate reagent kit and standards. Singleplex sets should
not be mixed with others from different panels or groups.
continues
310 | Bio-Plex Multiplex System In the U.S., call toll free at 1-800-424-6723
Bio-Plex Assays
bio-rad.com/bio-plex Bio-Plex Pro Cytokine, Chemokine, and Growth Factor Assays
Ordering Information
Catalog # Description Price, U.S. $
Human Th17 Premixed All-in-One Kit
171AA001M Bio-Plex Pro Human Th17 Cytokine 15-Plex Panel, 1 x 96-well, includes premixed coupled magnetic beads, 2,720.00
detection antibodies, standards, 2-level controls, detection antibody diluent, standard diluent HB, sample diluent
HB, assay buffer, wash buffer, streptavidin-PE, 96-well flat bottom plate, sealing tape, and instructions, for detecting
IL-1b, lL-4, IL-6, IL-10, IL-17A, IL-17F, IL-21, IL-22, IL-23, IL-25, IL-31, IL-33, IFN-g, sCD40L, TNF-a, (IL-17A/F
available as singleplex only)
Bio-Plex Pro Human Th17 Cytokine Singleplex Sets**, 1 x 96-Well
171BA001M IL-1b 215.00 171BA009M IL-23 215.00
171BA002M lL-4 215.00 171BA010M IL-25 215.00
171BA003M lL-6 215.00 171BA011M IL-31 215.00
171BA004M IL-10 215.00 171BA012M IL-33 215.00
171BA005M IL-17A 215.00 171BA013M IFN-g 215.00
171BA006M IL-17F 215.00 171BA014M sCD40L 215.00
171BA007M IL-21 215.00 171BA015M TNF-a 215.00
171BA008M IL-22 215.00 171BA016M IL-17A/F** 215.00
Standards
171DA0001 Bio-Plex Pro Human Th17 Cytokine Standard, pkg of 1 vial, lyophilized mixture of 16 standard analytes 195.00
171DA0501 io-Plex Pro Human Th17 Cytokine Standard, pkg of 50 lot-matched vials, lyophilized mixture of 16
B 7,710.00
standard analytes
Reagent Kit II — for use with human Th17 cytokine singleplex sets
171304055M Bio-Plex Pro Reagent Kit ll with Flat Bottom Plate, 1 x 96-well, includes detection antibody diluent, 395.00
standard diluent HB, sample diluent HB, assay and wash buffers, streptavidin-PE, flat bottom plate,
and sealing tape, for magnetic separation methods
Human Chemokine Premixed All-in-One Kit
171AK99MR2 Bio-Plex Pro Human Chemokine Panel 40-plex, 1 x 96-well, includes premixed coupled magnetic beads, 5,520.00
detection antibodies, standards, single-level controls, detection antibody diluent HB, standard diluent HB,
sample diluent HB, assay buffer, wash buffer, streptavidin-PE, 96-well flat bottom plate, sealing tape, and
instructions, for detecting 40 human chemokines shown below.
Bio-Plex Pro Human Cytokine Singleplex Sets, Group II**, 1 x 96-Well
171BK11MR2 6Ckine / CCL21 Set 210.00 171BK33MR2 IL-16 Set 210.00
171BK12MR2 BCA-1 / CXCL13 Set 210.00 171BK34MR2 IP-10 / CXCL10 Set 210.00
171BK13MR2 CTACK / CCL27 Set 210.00 171BK35MR2 I-TAC / CXCL11 Set 210.00
171BK14MR2 ENA-78 / CXCL5 Set 210.00 171BK36MR2 MCP-1 / CCL2 Set 210.00
171BK15MR2 Eotaxin / CCL11 Set 210.00 171BK37MR2 MCP-2 / CCL8 Set 210.00
171BK16MR2 Eotaxin-2 / CCL24 Set 210.00 171BK38MR2 MCP-3 / CCL7 Set 210.00
171BK17MR2 Eotaxin-3 / CCL26 Set 210.00 171BK39MR2 MCP-4 / CCL13 Set 210.00
171BK18MR2 Fractalkine / CX3CL1 210.00 171BK41MR2 MDC / CCL22 Set 210.00
171BK19MR2 GCP-2 / CXCL6 Set 210.00 171BK42MR2 MIF Set 210.00
171BK21MR2 GM-CSF Set 210.00 171BK43MR2 MIG / CXCL9 Set 210.00
171BK22MR2 Gro-a / CXCL1 Set 210.00 171BK44MR2 MIP-1a / CCL3 Set 210.00
171BK23MR2 Gro-b / CXCL2 Set 210.00 171BK46MR2 MIP-1d / CCL15 Set 210.00
171BK24MR2 I-309 / CCL1 Set 210.00 171BK47MR2 MIP-3a / CCL20 Set 210.00
171BK25MR2 IFN-g Set 210.00 171BK48MR2 MIP-3b / CCL19 Set 210.00
171BK26MR2 IL-1b Set 210.00 171BK49MR2 MPIF-1 / CCL23 Set 210.00
171BK27MR2 IL-2 Set 210.00 171BK51MR2 SCYB16 / CXCL16 Set 210.00
171BK28MR2 IL-4 Set 210.00 171BK52MR2 SDF-1a+b / CXCL12 Set 210.00
171BK29MR2 IL-6 Set 210.00 171BK53MR2 TARC / CCL17 Set 210.00
171BK31MR2 IL-8 / CXCL8 Set 210.00 171BK54MR2 TECK / CCL25 Set 210.00
171BK32MR2 IL-10 Set 210.00 171BK55MR2 TNF-a Set 210.00
Standards
171DK0001 io-Plex Pro Human Chemokine Standard, pkg of 1 vial, lyophilized mixture of 40 standard analytes
B 320.00
171DK0050 Bio-Plex Pro Human Chemokine Standard, pkg of 50 lot-matched vials, lyophilized mixture of 40 standard analytes Inquire
Reagent Kit III — for use with human cytokine singleplex sets from groups I and II
171304090M Bio-Plex Pro Reagent Kit III Flat Bottom Plate, 1 x 96-well, includes detection antibody diluent HB, standard 385.00
diluent HB, sample diluent HB, assay buffer, concentrated wash buffer, streptavidin-PE, flat bottom plate,
sealing tape, for magnetic separation methods
** Includes coupled magnetic beads and detection antibody. Requires the appropriate reagent kit and standards. Singleplex sets should
not be mixed with others from different panels or groups.
In the U.S., call toll free at 1-800-424-6723 Bio-Plex Multiplex System | 311
Bio-Plex Assays
Bio-Plex Pro Cytokine, Chemokine, and Growth Factor Assays bio-rad.com/bio-plex
Mouse Assays
Ordering Information
Catalog # Description Price, U.S. $
Mouse Cytokine Premixed All-in-One Kits*
M60000007A Bio-Plex Pro Mouse Cytokine 8-Plex Panel, 1 x 96-well, includes coupled magnetic beads, detection 1,625.00
antibodies, standards, assay buffer, wash buffer, detection antibody diluent, streptavidin-PE, flat bottom
plate, sealing tape, standard diluent, sample diluent, for the detection of IL-1b, IL-2, IL-4, IL-5, IL-10, GM-CSF,
IFN-g, TNF-a
MD000000EL Bio-Plex Pro Mouse Cytokine 9-Plex Panel, 1 x 96-well, includes coupled magnetic beads, detection 1,895.00
antibodies, standards, assay buffer, wash buffer, detection antibody diluent, streptavidin-PE, flat bottom
plate, sealing tape, standard diluent, sample diluent, for the detection of IL-15, IL-18, basic FGF, LIF, M-CSF,
MIG, MIP-2, PDGF-BB, VEGF
M60009RDPD Bio-Plex Pro Mouse Cytokine 23-Plex Panel, 1 x 96-well, includes coupled magnetic beads, detection 3,490.00
antibodies, standards, assay buffer, wash buffer, detection antibody diluent, streptavidin-PE, flat bottom
plate, sealing tape, standard diluent, sample diluent, for the detection of IL-1a, IL-1b, IL-2, IL-3, IL-4, IL-5, IL-6,
IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-17, eotaxin, G-CSF, GM-CSF, IFN-g, KC, MCP-1, MIP-1a, MIP-1b,
RANTES, TNF-a
M6000003J7 Bio-Plex Pro Mouse Cytokine Th1/Th2 Panel, 1 x 96-well, includes coupled magnetic beads, detection 1,635.00
antibodies, standards, assay buffer, wash buffer, detection antibody diluent, streptavidin-PE, flat bottom
plate, sealing tape, standard diluent, sample diluent, for the detection of IL-2, IL-4, IL-5, IL-10, IL-12 (p70),
GM-CSF, IFN-g, TNF-a
L6000004C6 Bio-Plex Pro Mouse Cytokine Th1 7-Plex Panel, 1 x 96-well, includes coupled magnetic beads, detection 2,475.00
antibodies, standards, assay buffer, wash buffer, detection antibody diluent, streptavidin-PE, flat bottom
plate, sealing tape, standard diluent, sample diluent, for the detection of IL-1b, IL-2, IL-6, IL-10, IL-12 (p70),
IFNa, TFN-a
L60000UKVT Bio-Plex Pro Mouse Cytokine Th2 7-Plex Panel, 1 x 96-well, includes coupled magnetic beads, detection 2,475.00
antibodies, standards, assay buffer, wash buffer, detection antibody diluent, streptavidin-PE, flat bottom
plate, sealing tape, standard diluent, sample diluent, for the detection of IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-13
Mouse Th17 Premixed All-in-One Kits
M6000007NY Bio-Plex Pro Mouse Cytokine Th17 Panel A 6-Plex Group l, 1 x 96-well, includes coupled magnetic beads, 1,310.00
detection antibodies, standard, assay buffer, wash buffer, detection antibody diluent, streptavidin-PE, flat bottom
plate, sealing tape, standard diluent, sample diluent, and instructions, for the detection of IL-1b, IL-6, IL-10, IL-17A,
IFN-g, TNF-a
171FA001M Bio-Plex Pro Mouse Cytokine Th17 Panel B 8-Plex Group lll, 1 x 96-well, includes coupled magnetic beads, 1,865.00
detection antibodies, standards, assay buffer, wash buffer, detection antibody diluent, streptavidin-PE, flat
bottom plate, sealing tape, standard diluent, sample diluent, for the detection of CD40L, IL-17F, IL-21, IL-22,
IL-23 (p19), IL-31, IL-33, MIP-3a
* Please contact your local Bio-Rad Sales Representative for pricing and availability of 10 x 96-well assay kits.
continues
312 | Bio-Plex Multiplex System In the U.S., call toll free at 1-800-424-6723
Bio-Plex Assays
bio-rad.com/bio-plex Bio-Plex Pro Cytokine, Chemokine, and Growth Factor Assays
Ordering Information
Catalog # Description Price, U.S. $ Catalog # Description Price, U.S. $
Bio-Plex Pro Mouse Cytokine Singleplex Sets, Group I*, 1 x 96-Well
171G5001M IL-1a Set 215.00 171G5013M IL-17A Set 215.00
171G5002M IL-1b Set 215.00 171G5014M Eotaxin Set 215.00
171G5003M IL-2 Set 215.00 171G5015M G-CSF Set 215.00
171G5004M IL-3 Set 215.00 171G5016M GM-CSF Set 215.00
171G5005M IL-4 Set 215.00 171G5017M IFN-g Set 215.00
171G5006M IL-5 Set 215.00 171G5018M KC Set 215.00
171G5007M IL-6 Set 215.00 171G5019M MCP-1 Set 215.00
171G5008M IL-9 Set 215.00 171G5020M MIP-1a Set 215.00
171G5009M IL-10 Set 215.00 171G5021M MIP-1b Set 215.00
171G5010M IL-12 (p40) Set 215.00 171G5022M RANTES Set 215.00
171G5011M IL-12 (p70) Set 215.00 171G5023M TNF-a Set 215.00
171G5012M IL-13 Set 215.00
Standards
171I50001 Bio-Plex Pro Mouse Cytokine Standards Group I, pkg of 1 vial, lyophilized mixture of 23 analytes 160.00
171I10501 Bio-Plex Pro Mouse Cytokine Standards Group I, pkg of 50 lot-matched vials, lyophilized mixture of 23 analytes 7,190.00
Bio-Plex Pro Mouse Cytokine Singleplex Sets, Group II*, 1 x 96-Well
171G6001M IL-15 Set 215.00 171G6005M MIG Set 215.00
171G6002M Basic FGF Set 215.00 171G6006M MIP-2 Set 215.00
171G6003M LIF Set 215.00 171G6007M PDGF-BB Set 215.00
171G6004M M-CSF Set 215.00 171G6008M VEGF Set 215.00
Standards
171I60001 Bio-Plex Pro Mouse Cytokine Standards Group II, pkg of 1 vial, lyophilized mixture of 9 analytes 115.00
171I10502 Bio-Plex Pro Mouse Cytokine Standards Group II, pkg of 50 lot-matched vials, lyophilized mixture of 5,535.00
9 analytes
Bio-Plex Pro Mouse Cytokine Singleplex Sets, Group III*, 1 x 96-Well
171GA001M CD40L Set 215.00 171GA007M IL-27 (p28) Set 215.00
171GA002M IL-17F Set 215.00 171GA008M IL-31 Set 215.00
171GA003M IL-21 Set 215.00 171GA009M IL-33 Set 215.00
171GA004M IL-22 Set 215.00 171GA010M ICAM-1 Set** 215.00
171GA005M IL-23 (p19) Set 215.00 171GA011M MIP-3a Set 215.00
171GA006M IL-25 Set 215.00
Standards
171IA0001 Bio-Plex Pro Mouse Cytokine Standards Group lll, pkg of 1 vial, lyophilized mixture for detecting 11 analytes 225.00
171IA0501 io-Plex Pro Mouse Cytokine Standards Group lll, pkg of 50 lot-matched vials, lyophilized mixture for
B 9,765.00
detecting 11 analytes
Reagent Kits — for use with mouse cytokine singleplex sets from Groups I, II, and III
171304070M Bio-Plex Pro Reagent Kit with Flat Bottom Plate, 1 x 96-well, includes flat bottom plate, assay buffer, wash 275.00
buffer, detection antibody diluent, streptavidin-PE, sealing tape, standard diluent, sample diluent, and instructions,
for magnetic separation methods
171304071 Bio-Plex Pro Reagent Kit, 10 x 96-well, includes assay buffer, wash buffer, detection antibody diluent, 2,395.00
streptavidin-PE, sealing tape, standard diluent, sample diluent
171304080M Bio-Plex Pro High Dilution Reagent Kit, 1 x 96-well, includes assay buffer, wash buffer, detection antibody 275.00
diluent, streptavidin-PE, flat bottom plate, sealing tape, standard diluent, sample diluent
* Includes coupled magnetic beads and detection antibody. Requires the appropriate reagent kit and standards. Singleplex sets should
not be mixed with others from different panels or groups.
** Requires high dilution reagent kit.
In the U.S., call toll free at 1-800-424-6723 Bio-Plex Multiplex System | 313
Bio-Plex Assays
Bio-Plex Pro Cytokine, Chemokine, and Growth Factor Assays bio-rad.com/bio-plex
Rat Assays
Ordering Information
Catalog # Description Price, U.S. $
Rat Cytokine Premixed All-in-One Kits*
171K1001M Bio-Plex Pro Rat Cytokine 24-Plex Panel, 1 x 96-well, includes coupled magnetic beads, detection 3,615.00
antibodies, standards, assay buffer, wash buffer, detection antibody diluent, streptavidin-PE, flat bottom plate,
sealing tape, standard diluent, sample diluent, for the detection of EPO, G-CSF, GM-CSF, Gro/KC, IFN-g, IL-1a,
IL-1b, IL-2, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12 (p70), IL-13, IL-17A, IL-18, MCP-1, M-CSF, MIP-1a, MIP-3a, RANTES,
TNF-a, VEGF
171K1002M Bio-Plex Pro Rat Cytokine Th1/Th2 12-Plex Panel, 1 x 96-well, includes coupled magnetic beads, detection 2,475.00
antibodies, standard, assay buffer, wash buffer, detection antibody diluent, streptavidin-PE, flat bottom plate,
sealing tape, standard diluent, sample diluent and instructions, for the detection of IL-a, IL-1b, IL-2, IL-4, IL-5,
IL-6, IL-10, IL-12 (p70), IL-13, GM-CSF, IFN-g, TNF-a
314 | Bio-Plex Multiplex System In the U.S., call toll free at 1-800-424-6723
Bio-Plex Assays
bio-rad.com/bio-plex Bio-Plex Pro Cell Signaling Assays
Ordering Information
Catalog # Description Price, U.S. $
Bio-Plex Pro TGF-b Premixed All-in-One Kit (compatible with Human, Mouse, and Rat samples)
171W4001M Bio-Plex Pro TGF-b 3-plex Assay, 1 x 96-well, includes coupled magnetic beads, detection antibodies, 925.00
standards, assay buffer, wash buffer, detection antibody diluent, streptavidin-PE, flat bottom plate, sealing tape,
standard diluent, sample diluent, for the detection of TGF-b1, TGF-b2, TGF-b3
Bio-Plex Pro TGF-b Singleplex Sets*, 1 x 96-Well
171V4001M TGF-b1 Set 240.00
171V4002M TGF-b2 Set 240.00
171V4003M TGF-b3 Set 240.00
Standards
171X40001 io-Plex Pro TGF-b Standard, pkg of 1 vial, lyophilized standard for detecting TGF-b1, TGF-b2, and
B 63.00
TGF-b3 analytes
171X40501 Bio-Plex Pro TGF-b Standard, pkg of 50 lot-matched vials, lyophilized standard for detecting TGF-b1, 4,135.00
TGF-b2, and TGF-b3 analytes
Reagent Kits — for use with rat cytokine and TGF-b singleplex sets
171304070 Bio-Plex Pro Reagent Kit, 1 x 96-well, includes assay buffer, wash buffer, detection antibody diluent, 275.00
streptavidin-PE, sealing tape, standard diluent, sample diluent, for vacuum separation methods
171304070M Bio-Plex Pro Reagent Kit with Flat Bottom Plate, 1 x 96-well, includes flat bottom plate, assay buffer, 275.00
wash buffer, detection antibody diluent, streptavidin-PE, sealing tape, standard diluent, sample diluent, and
instructions, for magnetic separation methods
171304071 Bio-Plex Pro Reagent Kit, 10 x 96-well, includes assay buffer, wash buffer, detection standards, assay buffer, 2,395.00
wash buffer, detection antibody diluent, streptavidin-PE, flat bottom plate, sealing tape, standard diluent, sample
diluent, for the detection of TGF-b1, TGF-b2, TGF-b3
Bio-Plex Pro Assay Accessories
171304500 Bio-Plex Wash Buffer, 1.5 L 96.00
171025001 Bio-Plex Pro Flat Bottom Plates**, 40 x 96-well plates 240.00
171304502 Filter Plate, 1 x 96-well filter plate with clear plastic lid, plate-holder tray, sealing tape not included 27.00
* Includes coupled magnetic beads and detection antibody. Requires the appropriate reagent kit and standards. Singleplex sets should
not be mixed with others from different panels or groups.
** Required for washing Bio-Plex Pro assays or other magnetic bead-based assays using Bio-Plex Pro and Bio-Plex Pro II wash stations.
Note: x-Plex assay panels can be ordered with any combination of available assays. For more information,
go to bio-rad.com/bio-plex/assaybuilder.
The Bio-Plex Pro cell signaling assays are magnetic bead– These assays incorporate several features to enhance
based immunoassays designed to meet the sensitivity needs both quality and ease of use:
of the most discerning scientists. The multiplex format enables ■■
Assay quick guide to get you started right away
robust, reproducible, and simultaneous measurement of ■■
ssay protocol optimized for exceptional sensitivity
A
proteins involved in key intracellular signaling pathways.
and broad dynamic range
Choose from a broad selection of phosphoprotein and total
protein targets to investigate pathways associated with cancer, ■■
lexible ordering options — order singleplex sets or
F
cardiovascular disorders, inflammation, drug mechanism of visit bio-rad.com/assaybuilder to configure a custom
action, diabetes, toxicology, and neurological disorders. premixed kit
In the U.S., call toll free at 1-800-424-6723 Bio-Plex Multiplex System | 315
Bio-Plex Assays
Bio-Plex Pro Cell Signaling Assays bio-rad.com/bio-plex
Available Assays
Phosphoprotein Lysate Control* Catalog #
Akt (Ser 473) GSK-3a/b (Ser 21/Ser 9)
Akt (Thr 308) MEK1 (Ser 217/Ser 221) EGF-treated HEK-293 171-YZ0001
202 204 185
Erk1/2 (Thr /Tyr , Thr /Tyr ) 187
245)
c-Abl (Tyr Untreated K-562 171-YZT003
Src (Tyr 416) Src-transfected NIH3T3 171-YZ0013
Negative control for all phosphoprotein assays Phosphatase-treated HeLa 171-YZB001
Total Target Lysate Control* Catalog #
Total Akt Total JNK Total p70 S6 Kinase
Total Erk 1/2 Total MEK1 Total PTEN
Untreated HeLa 171-YZT002
Total GSK-3b Total mTOR Total Smad2
Total IkB-a Total p38 MAPK Total IGF-1R
Total c-Jun
Untreated HEK-293 171-YZT001
Total CREB
Total Src Src-transfected NIH3T3 171-YZ0013
Total Btk H2O2-treated Ramos 171-YZ0011
Total ZAP-70 H2O2-treated Jurkat 171-YZ0012
Total HER-2 EFG-treated SK-BR-3 171-YZ0003
Negative control for all target assays Detection antibody diluent**
Housekeeping Protein Lysate Control* Catalog #
Human GAPDH Untreated HeLa 171-YZT002
b-Actin Untreated HeLa 171-YZT002
Negative control for all target assays Detection antibody diluent**
* All controls are shipped as one vial of lyophilized cell lysate.
** Included with the Bio-Plex Pro cell signaling reagent kit (#171304006M).
Ordering Information
Catalog # Description Price, U.S. $
Premixed All-in-One Kits
LQ00006JK0K0RR Bio-Plex Pro Cell Signaling Akt Panel 8-Plex Assay, 1 x 96-well 1,860.00
LQ00000S6KL81S Bio-Plex Pro Cell Signaling MAPK Panel 9-Plex Assay, 1 x 96-well 2,060.00
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316 | Bio-Plex Multiplex System In the U.S., call toll free at 1-800-424-6723
Bio-Plex Assays
bio-rad.com/bio-plex Bio-Plex Pro RBM Apoptosis Assays
Ordering Information
Catalog # Phosphoprotein Price, U.S. $ Catalog # Phosphoprotein Price, U.S. $
Phosphoprotein Singleplex Sets*
171V50001M Akt (Ser 473) 300.00 171V50033M mTOR (Ser2448) 300.00
171V50002M Akt (Thr308) 300.00 171V50013M NF-kB p65 (Ser 536) 300.00
171V50024M ATF-2 (Thr71) 300.00 171V50014M p38 MAPK (Thr180/Tyr182) 300.00
171V50025M BAD (Tyr223) 300.00 171V50034M p53 (Ser15) 300.00
171V50026M Btk (Tyr223) 300.00 171V50016M p70 S6 kinase (Thr389) 300.00
171V50027M c-Abl (Tyr245) 300.00 171V50015M p70 S6 kinase (Thr421/Ser 424) 300.00
171V50003M c-Jun (Ser63) 300.00 171V50035M p90 RSK (Ser380) 300.00
171V50028M CREB (Ser 133) 300.00 171V50017M PDGFR-a (Tyr754) 300.00
171V50004M EGFR (Tyr1068) 300.00 171V50018M PDGFR-b (Tyr751) 300.00
171V50005M EGFR (Tyr1173) 300.00 171V50036M PI3K p85 (Tyr458) 300.00
171V50006M Erk 1/2 (Thr202/Tyr204,Thr185/Tyr187) 300.00 171V50037M PTEN (Ser380) 300.00
171V50007M GSK-3a/b (Ser21/Ser9) 300.00 171V50038M S6 ribosomal protein (Ser235/Ser236) 300.00
171V50008M HER-2 (Tyr1248) 300.00 171V50019M Smad2 (Ser465/Ser 467) 300.00
171V50029M HSP27 (Ser 78) 300.00 171V50039M Src (Tyr416) 300.00
171V50009M IGF-1R (Tyr1131) 300.00 171V50020M Stat1 (Tyr701) 300.00
171V50031M IR-b (Tyr1146) 300.00 171V50021M Stat3 (Ser727) 300.00
171V50030M IRS-1 (Ser636/Ser639) 300.00 171V50022M Stat3 (Tyr705) 300.00
171V50010M IkB-a (Ser 32/Ser 36) 300.00 171V50040M Syk (Tyr352) 300.00
171V50011M JNK (Thr183/Tyr185) 300.00 171V50023M VEGFR-2 (Tyr1175) 300.00
171V50032M Lyn (Tyr507) 300.00 171V50041M ZAP-70 (Tyr319) 300.00
171V50012M MEK1 (Ser217/Ser221) 300.00
Total Target Singleplex Sets*
171V60001M Akt 300.00 171V60007M JNK 300.00
171V60012M Btk 300.00 171V60008M MEK1 300.00
171V60002M c-Jun 300.00 171V60015M mTOR 300.00
171V60013M CREB 300.00 171V60009M p38 MAPK 300.00
171V60003M Erk1/2 300.00 171V60010M p70 S6 kinase 300.00
171V60004M GSK-3b 300.00 171V60016M PTEN 300.00
171V60005M HER-2 300.00 171V60011M Smad2 300.00
171V60014M IGF-1R 300.00 171V60017M Src 300.00
171V60006M IkB-a 300.00 171V60018M ZAP-70 300.00
Housekeeping Protein Singleplex Sets*
171V60019M Human GAPDH 300.00 171V60020M b-Actin 300.00
Bio-Plex Pro Cell Signaling Reagents — cell signaling reagent kits are required to run the singleplex sets
171304006M Bio-Plex Pro Cell Signaling Reagent Kit, 1 x 96-well, includes assay cell lysis buffer, cell lysis factor QG, 405.00
cell wash buffer, bead resuspension buffer, detection antibody diluent, wash buffer, streptavidin-PE, 96-well
flat bottom plate, sealing tape, instructions
171304515 Bio-Plex Pro Cell Signaling Wash Buffer, 330 ml, for 1 x 96-well assay, for use with Bio-Plex Pro cell 33.00
signaling assays only, compatible with both magnetic and vacuum separation methods
* Lysate controls are recommended for singleplex sets.
Apoptosis Assays
In the U.S., call toll free at 1-800-424-6723 Bio-Plex Multiplex System | 317
Bio-Plex Assays
Bio-Plex Pro RBM Kidney Toxicity Assays bio-rad.com/bio-plex
Ordering Information
Catalog # Description Price, U.S. $
Bio-Plex Pro RBM Apoptosis Assays
171WAR1CK Bio-Plex Pro RBM Apoptosis Panel 1, 1 x 96-well all-in-one kit that includes premixed magnetic capture beads 1,380.00
and detection antibodies, standards, 2-level controls, standard diluent, buffers (blocking, lysate dilution (LDB),
cytosolic extraction (CEB), 10x assay), 10x streptavidin-PE, flat bottom plate, plate seals, and instructions, for the
detection of the following analytes in cell and tissue lysates: Bak, Bax, Lamin B, and Smac
171WAR2CK Bio-Plex Pro RBM Apoptosis Panel 2, 1 x 96-well all-in-one kit that includes premixed magnetic capture beads 1,540.00
and detection antibodies, standards, 2-level controls, standard diluent, buffers (blocking, lysate dilution (LDB),
cytosolic extraction (CEB), 10x assay), 10x streptavidin-PE, flat bottom plate, plate seals, and instructions, for the
detection of the following analytes in cell and tissue lysates: Bad, Bax / Bcl-2 dimer, Bcl-xL, Bim, and Mcl-1
171WAR3CK Bio-Plex Pro RBM Apoptosis Panel 3, 1 x 96-well all-in-one kit that includes premixed magnetic capture beads 1,380.00
and detection antibodies, standards, 2-level controls, standard diluent, buffers (blocking, lysate dilution (LDB),
cytosolic extraction (CEB), 10x assay), 10x streptavidin-PE, flat bottom plate, plate seals, and instructions,
for the detection of the following analytes in cell and tissue lysates: active caspase-3, Bcl-xL / Bak dimer,
Mcl-1 / Bak dimer, and survivin
Ordering Information
Catalog # Description Price, U.S. $
Bio-Plex Pro RBM Kidney Toxicity Assays
171ATR1CK Bio-Plex Pro RBM Human Kidney Toxicity Panel 1, 1 x 96-well, includes premixed magnetic capture beads, 1,480.00
premixed detection antibodies, standards mix, 2-level controls, blocking buffer, standard diluent, sample dilution
buffer, 10x assay buffer, 10x streptavidin-PE, 96-well flat bottom plate, plate seals, and instructions, for the
detection of the following analytes: calbindin, clusterin, GST-p, IL-18, KIM-1, MCP-1
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318 | Bio-Plex Multiplex System In the U.S., call toll free at 1-800-424-6723
Bio-Plex Assays
bio-rad.com/bio-plex Bio-Plex Pro Human Cancer Biomarker Panels
Ordering Information
Catalog # Description Price, U.S. $
171ATR2CK io-Plex Pro RBM Human Kidney Toxicity Panel 2, 1 x 96-well, includes premixed magnetic capture beads,
B 1,480.00
premixed detection antibodies, standards mix, 2-level controls, blocking buffer, standard diluent, sample dilution
buffer, 10x assay buffer, 10x streptavidin-PE, 96-well flat bottom plate, plate seals, and instructions, for the
detection of the following analytes: albumin, B2M, cystatin C, NGAL, osteopontin, TFF3
171QTR1CK Bio-Plex Pro RBM Canine Kidney Toxicity Panel 1, 1 x 96-well, includes premixed magnetic capture beads, 1,265.00
premixed detection antibodies, standards mix, 2-level controls, blocking buffer, standard diluent, sample dilution
buffer, 10x assay buffer, 10x streptavidin-PE, 96-well flat bottom plate, plate seals, and instructions, for the
detection of the following analytes: clusterin, KIM-1, MCP-1, NGAL
171QTR2CK Bio-Plex Pro RBM Canine Kidney Toxicity Albumin Kit, 1 x 96-well, includes premixed magnetic capture 800.00
beads, premixed detection antibodies, standards mix, 2-level controls, blocking buffer, standard diluent, sample
dilution buffer, 10x assay buffer, 10x streptavidin-PE, 96-well flat bottom plate, plate seals, and instructions,
for the detection of albumin
171KTR1CK Bio-Plex Pro RBM Rat Kidney Toxicity Panel 1, 1 x 96-well, includes premixed magnetic capture beads, 1,370.00
premixed detection antibodies, standards mix, 2-level controls, blocking buffer, standard diluent, sample dilution
buffer, 10x assay buffer, 10x streptavidin-PE, 96-well flat bottom plate, plate seals, and instructions, for the
detection of the following analytes: clusterin, IL-18, KIM-1, MCP-1, osteopontin
171KTR2CK Bio-Plex Pro RBM Rat Kidney Toxicity Panel 2, 1 x 96-well, includes premixed magnetic capture beads 1,265.00
premixed detection antibodies, standards mix, 2-level controls, blocking buffer, standard diluent, sample dilution
buffer, 10x assay buffer, 10x streptavidin-PE, 96-well flat bottom plate, plate seals, and instructions, for the
detection of the following analytes: B2M, calbindin, cystatin C, NGAL
171KTR3CK Bio-Plex Pro RBM Rat Kidney Toxicity Albumin Kit, 1 x 96-well, includes premixed magnetic capture beads, 800.00
premixed detection antibodies, standards mix, 2-level controls, blocking buffer, standard diluent, sample dilution
buffer, 10x assay buffer, 10x streptavidin-PE, 96-well flat bottom plate, plate seals, and instructions, for the
detection of albumin
In the U.S., call toll free at 1-800-424-6723 Bio-Plex Multiplex System | 319
Bio-Plex Assays
Bio-Plex Pro Human Cancer Biomarker Panels bio-rad.com/bio-plex
Available Human Cancer Biomarker Panel 1 Analytes Available Human Cancer Biomarker Panel 2 Analytes
sEGFR sIL-6Ra Prolactin Angiopoietin-2 IGFBP-1 TGF-a
FGF-basic Leptin SCF sCD40L IL-6 TNF-a
Follistatin Osteopontin sTIE-2 EGF IL-8 uPA
G-CSF PDGF-AB/BB sVEGFR-1 Endoglin IL-18* VEGF-A
sHER-2 / neu PECAM-1 sVEGFR-2 sFASL PAI-1 VEGF-C
HGF HB-EGF PLGF VEGF-D
* IL-18 is not available as a singleplex.
Ordering Information
Catalog # Description Price, U.S. $
Human Premixed All-in-One Kit
171AC500M Bio-Plex Pro Human Cancer Biomarker Panel 1, 16-Plex, 1 x 96-well, includes premixed coupled magnetic 2,815.00
beads, detection antibodies, standard, 2-level controls, detection antibody diluent, standard diluent HB, sample
diluent HB, assay and wash buffers, streptavidin-PE, 96-well flat bottom plate, sealing tape, and instructions,
for detection of sEGFR, FGF-basic, follistatin, G-CSF, sHER-2 / neu, HGF, sIL-6Ra, leptin, osteopontin,
PDGF-AB/BB, PECAM-1, prolactin, SCF, sTIE-2, sVEGFR-1, sVEGFR-2
171AC600M Bio-Plex Pro Human Cancer Biomarker Panel 2, 18-Plex, 1 x 96-well, includes premixed coupled magnetic 3,065.00
beads, detection antibodies, standard, 2-level controls, detection antibody diluent, standard diluent HB, sample
diluent HB, assay and wash buffers, streptavidin-PE, 96-well flat bottom plate, sealing tape, and instructions,
for detection of angiopoietin-2, sCD40L, EGF, endoglin, sFASL, HB-EGF, IGFBP-1, IL-6, IL-8, IL-18, PAI-1,
PLGF, TGF-a, TNF-a, uPA, VEGF-A, VEGF-C, VEGF-D
Catalog # Description Price, U.S. $ Catalog # Description Price, U.S. $
Bio-Plex Pro Human Cancer Biomarker Panel 1 Singleplex Sets*, 1 x 96-Well
(receptors, cytokines, chemokines, growth factors, and hormones)
171BC501M sEGFR Set 215.00 171BC509M Osteopontin Set 215.00
171BC502M FGF-basic Set 215.00 171BC511M DGF-AB/BB Set
P 215.00
171BC503M Follistatin Set 215.00 171BC510M PECAM-1 Set 215.00
171BC504M G-CSF Set 215.00 171BC512M Prolactin Set 215.00
171BC505M HGF Set 215.00 171BC513M SCF Set 215.00
171BC506M sHER-2 / neu Set 215.00 171BC514M sTIE-2 Set 215.00
171BC507M sIL-6Ra Set 215.00 171BC515M sVEGFR-1 Set 215.00
171BC508M Leptin Set 215.00 171BC516M sVEGFR-2 Set 215.00
Standards
171DC5000 io-Plex Pro Human Cancer Biomarker Panel 1, 16-Plex Standards, pkg of 1 vial, lyophilized mixture of
B 195.00
16 standard analytes
171DC5001 Bio-Plex Pro Human Cancer Biomarker Panel 1, 16-Plex Standards, pkg of 50 lot-matched vials, 8,255.00
lyophilized mixture of 16 standard analytes
Bio-Plex Pro Human Cancer Biomarker Panel 2 Singleplex Sets*, 1 x 96-Well
(ligands, cytokines, chemokines, and growth factors)
171BC601M Angiopoietin-2 Set 215.00 171BC611M PAI-1 Set 215.00
171BC602M sCD40L Set 215.00 171BC612M PLGF Set 215.00
171BC603M EGF Set 215.00 171BC613M TGF-a Set 215.00
171BC604M Endoglin Set 215.00 171BC614M TNF-a Set 215.00
171BC605M sFASL Set 215.00 171BC615M uPA Set 215.00
171BC606M HB-EGF Set 215.00 171BC616M VEGF-A Set 215.00
171BC607M IGFBP-1 Set 215.00 171BC617M VEGF-C Set 215.00
171BC608M IL-6 Set 215.00 171BC618M VEGF-D Set 215.00
171BC609M IL-8 Set 215.00
Standards
171DC6000 io-Plex Pro Human Cancer Biomarker Panel 2, 18-Plex Standards, pkg of 1 vial, lyophilized mixture of
B 185.00
18 standard analytes
171DC6001 Bio-Plex Pro Human Cancer Biomarker Panel 2, 18-Plex Standards, pkg of 50 lot-matched vials, 9,280.00
lyophilized mixture of 18 standard analytes
Reagent Kits II — for use with human cancer biomarker panels 1 and 2
171304055M Bio-Plex Pro Reagent Kit ll with Flat Bottom Plate, 1 x 96-well, includes detection antibody diluent, 395.00
standard diluent HB, sample diluent HB, assay and wash buffers, streptavidin-PE, flat bottom plate,
and sealing tape, for use with magnetic separation methods
* Includes coupled magnetic beads and detection antibody. Requires the appropriate reagent kit and standards.Singleplex sets should
not be mixed with others from different panels or groups.
320 | Bio-Plex Multiplex System In the U.S., call toll free at 1-800-424-6723
Bio-Plex Assays
bio-rad.com/bio-plex Bio-Plex Pro Diabetes Assays
Diabetes Assays
In the U.S., call toll free at 1-800-424-6723 Bio-Plex Multiplex System | 321
Bio-Plex Assays
Bio-Plex Pro Diabetes Assays bio-rad.com/bio-plex
Ordering Information
Catalog # Description Price, U.S. $
Human Premixed All-in-One Kits (compatible with non-human primate samples)
171A7001M Bio-Plex Pro Human Diabetes 10-Plex Panel, 1 x 96-well, includes premixed coupled magnetic beads, 2,125.00
detection antibodies, standards, assay and wash buffers, detection antibody diluent, streptavidin-PE, flat
bottom plate, sealing tape, standard and sample diluents for the detection of C-Peptide, ghrelin, GIP, GLP-1,
glucagon, insulin, leptin, PAI-1, resistin, and visfatin
171A7002M Bio-Plex Pro Human Diabetes Adipsin and Adiponectin Assays, 1 x 96-well, includes coupled magnetic 880.00
beads, detection antibodies, standards, assay and wash buffers, detection antibody diluent, streptavidin-PE,
flat bottom plate, sealing tape, serum-based diluent for the detection of adipsin and adiponectin
171A7003M Bio-Plex Pro Human Diabetes Adiponectin Assay, 1 x 96-well, includes coupled magnetic beads, detection 460.00
antibodies, standards, assay and wash buffers, detection antibody diluent, streptavidin-PE, flat bottom plate,
sealing tape, serum-based diluent for the detection of adiponectin
171A7004M Bio-Plex Pro Human Diabetes Adipsin Assay, 1 x 96-well, includes coupled magnetic beads, detection 465.00
antibodies, standards, assay and wash buffers, detection antibody diluent, streptavidin-PE, flat bottom
plate, sealing tape, serum-based diluent for the detection of adipsin
322 | Bio-Plex Multiplex System In the U.S., call toll free at 1-800-424-6723
Bio-Plex Assays
bio-rad.com/bio-plex Bio-Plex Pro Human Isotyping Assays
Ordering Information
Catalog # Description Price, U.S. $ Catalog # Description Price, U.S. $
Bio-Plex Pro Mouse Diabetes Singleplex Sets*, 1 x 96-Well
171G7002M Ghrelin Set 270.00 171G7006M Insulin Set 270.00
171G7003M GIP Set 270.00 171G7007M Leptin Set 270.00
171G7004M GLP-1 Set 270.00 171G7008M PAI-1 Set 270.00
171G7005M Glucagon Set 270.00 171G7009M Resistin Set 270.00
Standards
171I70001 Bio-Plex Pro Mouse Diabetes Standards, pkg of 1 vial, lyophilized mixture of 9 standard analytes, includes 160.00
adiponectin, ghrelin, GIP, GLP-1, glucagon, insulin, leptin, PAI-1, and resistin
171I70050 Bio-Plex Pro Mouse Diabetes Standards, pkg of 50 lot-matched vials, lyophilized mixture of 9 standard 6,960.00
analytes, includes adiponectin, ghrelin, GIP, GLP-1, glucagon, insulin, leptin, PAI-1, and resistin
Bio-Plex Pro Rat Diabetes Singleplex Sets*, 1 x 96-Well
171L7001M Ghrelin Set 270.00 171L7006M Leptin Set 270.00
171L7003M GLP-1 Set 270.00 171L7007M AI-1 Set
P 270.00
171L7004M Glucagon Set 270.00
Standards
171NZ0001 io-Plex Pro Rat Standards, pkg of 1 vial, lyophilized mixture of 31 standard analytes. Compatible with rat
B 225.00
cytokine and rat diabetes assays
171NZ0501 Bio-Plex Pro Rat Standards, pkg of 50 lot-matched vials, lyophilized mixture of 31 standard analytes. 8,975.00
Compatible with rat cytokine and rat diabetes assays
Reagent Kit — for use with diabetes singleplex sets
171304070M Bio-Plex Pro Reagent Kit with Flat Bottom Plate, 1 x 96-well, includes flat bottom plate, assay and wash 275.00
buffers, detection antibody diluent, streptavidin-PE, sealing tape, standard and sample diluents, and instructions,
for use with magnetic separation methods
* Includes coupled magnetic beads and detection antibody. Requires the appropriate reagent kit and standards. Singleplex sets should
not be mixed with others from different panels or groups.
In the U.S., call toll free at 1-800-424-6723 Bio-Plex Multiplex System | 323
Bio-Plex Assays
Bio-Plex Pro Human Acute Phase Assay Panel bio-rad.com/bio-plex
Ordering Information
Catalog # Description Price, U.S. $
Bio-Plex Pro Human Isotyping Panel Premixed All-in-One Multiplex Kit
171A3100M Bio-Plex Pro Human Isotyping Panel, 6-plex, 1 x 96-well, includes coupled magnetic beads, detection 1,335.00
antibodies, standards, 1-level quality controls, detection antibody diluent, isotyping diluent (for use with samples,
standards, and controls), assay buffer, wash buffer, streptavidin-PE, 96-well flat bottom plate, sealing tape, assay
quick guide, and product data sheet, for detection of IgG1, IgG2, IgG3, IgG4, IgA, and IgM
Bio-Plex Pro Human Isotyping All-in-One Singleplex Kits*
171A3101M IgA Kit, 1 x 96-well 535.00
171A3104M IgM Kit, 1 x 96-well 535.00
171A3102M IgE Kit, 1 x 96-well 535.00
171A3103M IgG Total Kit, 1 x 96-well 655.00
* Singleplex kits are specific for each assay indicated and include coupled magnetic beads, detection antibodies, standards,
1-level quality controls, detection antibody diluent, isotyping diluent (for use with samples, standards, and controls), assay buffer,
wash buffer, streptavidin-PE, 96-well flat bottom plate, sealing tape, assay quick guide, and product data sheet.
Inflammation in response to tissue injury, infection, sepsis, Available Bio-Plex Pro Human Acute Phase Assays
cardiovascular disease, and diabetes typically involves the a-2-macroglobulin Procalcitonin
production and release of multiple acute phase proteins from CRP SAA
Ferritin SAP
the liver. The Bio-Plex Pro human acute phase assay panels Fibrinogen Tissue plasminogen activator
deliver accurate and reproducible measurement of nine of the Haptoglobin
most commonly measured acute phase biomarkers in just
3 hours with as little as 13 µl of sample. Bio-Plex Pro human
■■
Compatible with the Bio-Plex® 200 system
acute phase assay features include: and all Luminex 100- and 200-based readers
(uses 25 bead map)
■■
io-Plex Pro human acute phase 5-plex assay panel —
B
includes ferritin, fibrinogen, procalcitonin, serum For optimal performance, the acute phase panel exists
amyloid A, and tissue plasminogen activator as separate 5-plex and 4-plex assays with distinct
sample dilution requirements.
■■
io-Plex Pro human acute phase 4-plex assay panel —
B
includes a-2-macroglobulin, CRP, haptoglobin, and For More Information
Web: bio-rad.com/bio-plexproacutephase
serum amyloid P
Request or download bulletin: 5650
■■
Validated in serum, plasma, and culture supernatant
Ordering Information
Catalog # Description Price, U.S. $
Complete Kits*
171A4S07M Bio-Plex Pro Human Acute Phase 5- + 4-Plex Panel Complete Kit, 1 x 96-well 2,195.00
171A4C09M Bio-Plex Pro Human Acute Phase 4-Plex Panel Complete Kit, 1 x 96-well 1,155.00
171A4C07M Bio-Plex Pro Human Acute Phase 5-Plex Panel Complete Kit, 1 x 96-well 1,400.00
* Kits are specific for each assay indicated and include coupled magnetic beads, detection antibodies, standards, 1-level quality
controls, detection antibody diluent, isotyping diluent (for use with samples, standards, and controls), assay buffer, wash buffer,
streptavidin-PE, 96-well flat bottom plate, sealing tape, assay quick guide, and product data sheet.
continues
324 | Bio-Plex Multiplex System In the U.S., call toll free at 1-800-424-6723
Bio-Plex Assays
bio-rad.com/bio-plex Bio-Plex COOH Beads and Related Reagents
Ordering Information
Catalog # Description Price, U.S. $
Assay Kits*
171A4007M Bio-Plex Pro Human Acute Phase 5-Plex Panel, 1 x 96-well, includes premixed coupled magnetic beads, 980.00
detection antibodies, standards, and controls for the detection of ferritin, fibrinogen, procalcitonin, serum amyloid
A, tissue plasminogen activator; requires acute phase reagent kit and acute phase diluent kit
171A4008M Bio-Plex Pro Human Acute Phase 5-Plex Panel, 10 x 96-well, requires 10 acute phase reagent kits and 9,400.00
10 acute phase diluent kits
171A4009M Bio-Plex Pro Human Acute Phase 4-Plex Panel, 1 x 96-well, includes premixed coupled magnetic beads, 850.00
detection antibodies, standards, and controls for the detection of a-2-macroglobulin, CRP, haptoglobin, serum
amyloid P; requires acute phase reagent kit and acute phase diluent kit
171A4010M Bio-Plex Pro Human Acute Phase 4-Plex Panel, 10 x 96-well, requires 10 acute phase reagent kits and 7,605.00
10 acute phase diluent kits
Standards
171D40002 Bio-Plex Pro Human Acute Phase Standards, pkg of 2 vials, lyophilized mixture of 9 standard antigens 260.00
171D40006 Bio-Plex Pro Human Acute Phase Standards, pkg of 50 lot-matched vials, lyophilized mixture of 5,725.00
9 standard antigens
Reagent Kit
171304050 io-Plex Pro Human Acute Phase Reagent Kit, 1 x 96-well, includes assay buffer, wash buffer,
B 150.00
detection antibody diluent, streptavidin-PE, filter plate, sealing tape
Diluent Kit**
171305050 io-Plex Pro Human Acute Phase Diluent Kit, 1 x 96-well, includes 10 ml serum-based diluent and
B 145.00
300 ml serum-free diluent
* One acute phase reagent kit is required for use with each assay kit.
** One acute phase diluent kit is sufficient to perform one 5-plex and one 4-plex assay.
Ordering Information
Catalog # Description Price, U.S. $
MC100xx01* Bio-Plex Pro Magnetic COOH Beads, 1 ml 685.00
MC100xx04* Bio-Plex Pro Magnetic COOH Beads, 4 ml 2,720.00
* xx = bead region. Bead regions available: 26–29, 34–37, 43–46, 52–55, and 62–65.
In the U.S., call toll free at 1-800-424-6723 Bio-Plex Multiplex System | 325
Bio-Plex Assays
Bio-Plex COOH Beads and Related Reagents bio-rad.com/bio-plex
Ordering Information
Catalog # Description Price, U.S. $
1715060xx* Bio-Plex Nonmagnetic COOH Beads, 1.25 x 107 beads/ml, 1 ml 410.00
1716060xx* Bio-Plex Nonmagnetic COOH Beads, 16 ml 5,330.00
* xx = bead region. Bead regions available, 11, 16, 18, 20, 24–28, 30, 31, 33, 35, 38, 42–46, 50–53, 56, and 66.
Ordering Information
Catalog # Description Price, U.S. $
Bio-Plex Amine Coupling Kit
171406001* Bio-Plex Amine Coupling Kit, 30 reactions, includes bead wash buffer, bead activation buffer, 535.00
storage buffer, staining buffer, coupling reaction tubes
Bio-Plex Streptavidin-PE**
171304501 Bio-Plex Streptavidin-PE, streptavidin-phycoerythrin for 10 x 96-well reactions 625.00
* For protein coupling only.
** Streptavidin-PE is a component of all reagent kits and is available seperately.
326 | Bio-Plex Multiplex System In the U.S., call toll free at 1-800-424-6723
Bio-Plex Multiplex System
Subject Index
Subject Index
Apoptosis Assays, 317
Bio-Plex® 3D Multiplex System, 300
Bio-Plex® 200 System, 299
Bio-Plex® Amine Coupling Kit , 326
Bio-Plex Data Pro™ Software, 304
Bio-Plex® MAGPIX™ Multiplex Reader Accessories and Software, 301
Bio-Plex Manager™ Software Conversions and Upgrades, 303
Bio-Plex Manager™ Software, Security Edition, 304
Bio-Plex Manager™ Software, Standard Edition, 302
Bio-Plex® Nonmagnetic COOH Beads, 326
Bio-Plex Pro™ Human Inflammation Assays, 307
Bio-Plex Pro™ Human MMP and TIMP Assays, 305
Bio-Plex Pro™ Magnetic COOH Beads, 325
Bio-Plex Pro™ RBM Human Metabolic and Hormone Panels, 306
Bio-Plex Pro™ Washing Accessories, 301
Cell Signaling Assays, 315
Diabetes Assays, 321
Human Acute Phase Assay Panel, 324
Human Assays, 309
Human Cancer Biomarker Panels, 319
Human Isotyping Assays, 323
Kidney Toxicity Assays, 318
Mouse Assays, 312
Multi-Species TGF-b Assays, 315
Rat Assays, 314
In the U.S., call toll free at 1-800-424-6723 Bio-Plex Multiplex System | 327
328 | Bio-Plex Multiplex System In the U.S., call toll free at 1-800-424-6723
Microplate Systems
Microplate Absorbance Readers and Accessories 330
Instruments and Software 330
ELISA Reagents and Kits 332
Microplate Absorbance Readers and Accessories
Instruments and Software bio-rad.com/microplate
Ordering Information
Catalog # Description Price, U.S. $
1681130 iMark Microplate Absorbance Reader, 100/240 V, includes 6 filters (415, 450, 490, 595, 655, 750 nm), 5,735.00
built-in plate shaker, onboard software and thermal printer, one roll printer paper, USB2 and power cables
1681135 iMark Microplate Absorbance Reader with Microplate Manager 6 Software, for PC and MAC 6,620.00
Accessories
1660495 -Channel Professional Adjustable-Volume 20–200 µl Digital Micropipet, pkg of 1, 8-channel
8 698.75
20–200 µl adjustable-volume micropipet, includes volume lock mechanism, curved tip ejector,
rotating manifold, fully autoclavable
1686940 Checkmark Reader Performance Verification Kit, includes absorbance calibration plate, Checkmark software 2,450.00
1682230 IQ/OQ Kit for iMark Microplate Absorbance Reader, includes #1686940, IQ/OQ protocols 3,005.00
Ordering Information
Catalog # Description Price, U.S. $
1681150 Mark Microplate Absorbance Spectrophotometer, PC or Mac, built-in incubator, plate shaker,
x Inquire
Microplate Manager 6 software, USB2 and power cables
1682000 Plate Adaptor, for using Terasaki plate with xMark microplate absorbance spectrophotometer 515.00
Accessories
1682250 IQ/OQ Kit for xMark Microplate Absorbance Spectrophotometer, includes #1686940, IQ/OQ protocols 2,615.00
Bio-Rad’s iMark™ microplate reader and xMark™ microplate of data analysis options to include kinetics and screening,
spectrophotometer can be utilized to their full potential and adds the functionality of flexible template creation for
with Microplate Manager 6 software. This comprehensive any standard microplate format up to 1,536 wells and for
software package allows powerful, versatile colorimetric 60- and 70-well Terasaki plates. The custom reporting
and turbidimetric analyses and report analysis for raw function provides one-button screening for predefined
data, qualitative, and quantitative calculations based on assays such as those for TSE screening.
absorbance limits, matrix of equations, normalization, and For More Information
curve fit. Microplate Manager software expands your range Web: bio-rad.com/microplatemanager
Ordering Information
Catalog # Description Price, U.S. $
1689520 icroplate Manager 6 Software, PC and Mac, for end-point and kinetic data analysis with
M 2,065.00
iMark microplate absorbance reader and xMark microplate spectrophotometer
Ordering Information
Catalog # Description Price, U.S. $
1707009 ImmunoWash 1575 Microplate Washer, 110–240 V, includes bottle and tubing set, 8-port manifold, 6,910.00
aerosol protection cover
1707021 ImmunoWash 1575 12-Channel Manifold 475.00
1707026 ImmunoWash 1575 Standard Maintenance Kit 165.00
Ordering Information
Catalog # Description Price, U.S. $
Enzyme-Antibody Conjugates
1721011 Goat Anti-Mouse IgG (H + L)-HRP Conjugate, 2 ml 165.00
1721033 Goat Anti-Human IgG (g)-HRP Conjugate, 1 ml 235.00
1721019 Goat Anti-Rabbit IgG (H + L)-HRP Conjugate, 1 ml 94.00
1721034 Rabbit Anti-Goat IgG (H + L)-HRP Conjugate, 1 ml 185.00
1721017 Rabbit Anti-Sheep IgG (H + L)-HRP Conjugate, 1 ml 170.00
1721037 Rabbit Anti-Goat IgG (H + L)-AP Conjugate, 1 ml 225.00
Substrate Systems
1721063 AP Substrate Kit, contains 100 pNPP tablets and 100 ml 5x concentrate diethanolamine buffer 230.00
1721064 HRP Substrate Kit, contains 180 ml ABTS, 20 ml hydrogen peroxide 175.00
1721066 TMB Peroxidase EIA Substrate Kit, contains 180 ml TMB solution, 20 ml hydrogen peroxide 180.00
1721067 TMB Peroxidase EIA Substrate Kit, contains 900 ml TMB solution, 100 ml hydrogen peroxide 485.00
1721068 Single-Component TMB Peroxidase EIA Substrate Kit, contains 100 ml TMB solution 81.00
1721072 Single-Component TMB Peroxidase ELISA Substrate Kit, contains 1 L TMB solution 710.00
This kit provides a convenient ELISA method for identifying the is reacted with eight subclass-specific antisera to determine
class, subclass, and light-chain type of mouse IgGs in tissue the heavy- and light-chain types of the monoclonal antibody.
culture supernatant and ascites fluid. The kit allows screening For More Information
of up to 12 different antibody samples per plate. Each sample Web: bio-rad.com/microplatereagents
Ordering Information
Catalog # Description Price, U.S. $
1722051 Mouse Typer Isotyping Kit, includes mouse isotyping panel, affinity purified goat anti-rabbit IgG (H + L) 545.00
1722055 Mouse Typer Isotyping Panel, includes 10 ml each ultrapure rabbit anti-mouse subclass-specific antiserum 360.00
to mouse IgG1, IgG2a, IgG2b, IgG3, IgM, IgA, k-chain, and l-chain
Subject Index
ELISA Reagents, 332
iMark™ Microplate Absorbance Reader, 330
ImmunoWash™ 1575 Microplate Washer, 331
Microplate Manager® 6 Software, 331
Mouse Antibody Isotyping Kit, 332
xMark™ Microplate Absorbance Spectrophotometer , 330
Transfection Technologies
Introducing DNA and RNA into cells is a powerful tool for evaluating gene expression. Bio-Rad’s transfection products offer
choices for gene delivery to bacterial, fungal, plant, and animal cells. Use the guide below to select the most appropriate
transfection technology for your application.
Learn More about the Technology
Web: bio-rad.com/tech/transfection
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Transfection Technologies Page Plants Bacteria Fungi Animals
Lipid Transfection
siLentFect™ lipid reagent for RNAi 337 ° ° • • • • •
TransFectin™ lipid reagent 338 ° ° • • • • •
Electroporation
Gene Pulser Xcell™ total system 340 •*
•* ° ° • • • • • • • •
Gene Pulser Xcell eukaryotic system 340 •*
•* ° ° ° ° ° ° • • • •
Gene Pulser Xcell microbial system 340 • • • •
MicroPulser™ electroporator 342 • • • •
Biolistic Particle Delivery
Helios® gene gun system 345 •
• •
• •
° ° • • • • • • ° • • • •
•
PDS-1000/He™ system 346 •
• •
• • •
° ° •
• •
° • • •
•
PDS-1000/He system with
Hepta™ adaptor
347 •
• •
• • •
° ° •
• • ° • • • •
Lipid A
Sample
DNA-coated
microcarrier
Transfection
Complexing
■■
Effective gene-specific silencing — achieve 90–99%
gene-specific knockdown of both high- and low-abundance
genes using as little as 1 nM siRNA for certain gene targets
■■
Low amounts of siRNA and lipid required — the high ■■
otransfection capabilities — simultaneous delivery
C
affinity of siLentFect lipid reagent for siRNA allows the use of siRNA and dsDNA vectors for optimization and dual
of less lipid and less siRNA per experiment, decreasing the expression analysis
likelihood of off-target effects, reducing cost, and minimizing ■■
orks with many cell types — 184htrt, 4T1, A549,
W
the experimental bias caused by cell stress/death Caco-2, CHO-K1, COS-7, HEK 293, HeLa, HepG2,
■■
Simple, flexible protocol — easily adaptable protocol HUVEC, LNCaP, MCF-7, murine EC, NIH 3T3, PC-3,
for high-throughput applications; successfully transfect primary fibroblast, primary keratinocyte, primary ovarian,
cells by adding siLentFect reagent and siRNA directly to SVEC4, VSMC
the culture or by adding siLentFect-siRNA complexes to For More Information
trypsinized cells still in suspension Web: bio-rad.com/silenfect
Request or download bulletins: 3105, 5439, and 5894
Ordering Information
Catalog # Description Price, U.S. $
1703360 siLentFect Lipid Reagent for RNAi, 0.5 ml 230.00
1703361 siLentFect Lipid Reagent for RNAi, 1.0 ml 400.00
1703362 siLentFect Lipid Reagent for RNAi, 5 x 1.0 ml 1,515.00
Ordering Information
Catalog # Description Price, U.S. $
1703350 TransFectin Lipid Reagent, 0.5 ml 190.00
1703351 TransFectin Lipid Reagent, 1.0 ml 355.00
1703352 TransFectin Lipid Reagent, 5 x 1.0 ml 1,310.00
decreases over time exponentially. The pulse length is the time the
The time required for the initial t
cells are subjected to the discharge.
V0 voltage to drop to V0 /e is referred A slight drop in voltage occurs
e with all square-wave instruments.
to as the time constant (t) and is a
convenient expression of the This drop in voltage is called the
t pulse length. Time, ms pulse droop and is measured as a
Time, ms
percentage of the initial voltage.
A Choice of Exponential-Decay or Square-Wave Pulse have been used effectively for electroporation. Depending
The Gene Pulser Xcell™ electroporation system generates on cell type, the shape of the wave can have a significant
both exponential and square waveforms, allowing you to effect on the transfection efficiency. Electrofusion is also
choose the waveform and protocol that work best for your possible with the Gene Pulser ® system.
cells. Both exponential-decay and square-wave pulses
Ordering Information
Catalog # Description Price, U.S. $
1652676 ene Pulser Electroporation Buffer, 10 x 1.8 ml
G 315.00
1652677 Gene Pulser Electroporation Buffer, 30 ml 410.00
The Gene Pulser Xcell system is a modular electroporation Pulse Controller Module
Gene Pulser Xcell For the electroporation of most eukaryotic cells, including mammalian cells and plant
Eukaryotic System protoplasts; includes the CE module.
Gene Pulser Xcell For the electroporation of bacteria and fungi as well as other applications where high-voltage
Microbial System pulses are applied to samples of small volume; includes the PC module.
Ordering Information
Catalog # Description Price, U.S. $
1652660 ene Pulser Xcell Total System, for mammalian and microbial cells, 100/240 V, 50/60 Hz, exponential-
G 7,770.00
decay and square-wave delivery, includes main unit, CE module, PC module, ShockPod cuvette chamber,
15 sterile cuvettes (5 each of 0.1, 0.2, and 0.4 cm gap), cuvette rack
1652661 Gene Pulser Xcell Eukaryotic System, 100/240 V, 50/60 Hz, exponential-decay (25–3,275 µF range) 7,210.00
and square-wave delivery, includes main unit, CE module, ShockPod cuvette chamber, 5 sterile cuvettes
(0.4 cm gap), cuvette rack
1652662 Gene Pulser Xcell Microbial System, 100/240 V, 50/60 Hz, exponential-decay delivery, includes main unit, 6,480.00
PC module, ShockPod cuvette chamber, 10 sterile cuvettes (5 each of 0.1 and 0.2 cm gap), cuvette rack
Components
1652666 Gene Pulser Xcell Main Unit, 100/240 V, 50/60 Hz 5,795.00
1652667 Gene Pulser Xcell CE Module, 25–3,275 µF range controlled by main unit, includes integral leads 1,970.00
1652668 Gene Pulser Xcell PC Module, 50–1,000 W range controlled by main unit, includes integral leads 1,020.00
1652669 Gene Pulser Xcell ShockPod Cuvette Chamber, includes integral leads for connection to 625.00
Gene Pulser Xcell, Gene Pulser II, or MicroPulser electroporators
1652095 Gene Pulser Cuvette Rack 44.00
Electroporation Plates
Electroporation plates for use with the Gene Pulser MXcell
electroporation system are available in three formats: 96-well
for low-volume or screening experiments, and 24- or 12-well.
Benefits include:
■■
Streamlined optimization — up to 24 different protocols
can be delivered on a single 96- or 24-well plate
■■
High reproducibility — well-to-well and plate-to-plate 96-Well Electroporation Plate
variability is less than 20%
■■
Scalability — consistent transfection efficiency across
96-, 24-, and 12-well plate formats
For More Information
Web: bio-rad.com/mxcellplates
Ordering Information
Catalog # Description Price, U.S. $
Electroporation Plates
1652681 96-Well Electroporation Plate 205.00
1652682 24-Well Electroporation Plate 200.00
1652683 12-Well Electroporation Plate 200.00
MicroPulser™ Electroporator
Ordering Information
Catalog # Description Price, U.S. $
1652100 icroPulser Electroporator, includes a cuvette chamber with leads, 10 sterile cuvettes
M 2,215.00
(5 each of 0.1 cm and 0.2 cm gap)
Ordering Information
Catalog # Description Price, U.S. $
Standard Packs
1652088 Gene Pulser/MicroPulser Cuvettes, 0.4 cm gap, 50 180.00
1652086 Gene Pulser/MicroPulser Cuvettes, 0.2 cm gap, 50 180.00
1652089 Gene Pulser/MicroPulser Cuvettes, 0.1 cm gap, 50 180.00
Jumbo Packs*
1652091 Gene Pulser/MicroPulser Cuvettes, 0.4 cm gap, 500 1,330.00
1652092 Gene Pulser/MicroPulser Cuvettes, 0.2 cm gap, 500 1,330.00
1652093 Gene Pulser/MicroPulser Cuvettes, 0.1 cm gap, 500 1,330.00
Mini Packs
1652081 Gene Pulser/MicroPulser Cuvettes, 0.4 cm gap, 5 15.00
1652082 Gene Pulser/MicroPulser Cuvettes, 0.2 cm gap, 5 15.00
1652083 Gene Pulser/MicroPulser Cuvettes, 0.1 cm gap, 5 15.00
* Please inquire about volume pricing.
Ordering Information
Catalog # Description Price, U.S. $
1652431* elios Gene Gun System, 100/120 V, includes Helios gene gun kit, helium hose assembly, helium
H Inquire
regulator, tubing prep station, syringe kit, Tefzel tubing, tubing cutter, Helios gene gun optimization kit
1652432 Helios Gene Gun System, 220/240 V Inquire
1652451 Helios Gene Gun Low-Pressure System, 100/120 V, same as #1652431 with low-pressure regulator Inquire
(maximum 400 psi)
1652452 Helios Gene Gun Low-Pressure System, 220/240 V Inquire
* Additional items required for operation of the Helios gene gun system: helium tank, grade 4.5 (99.995% pure) or higher,
pressurized to the desired output pressure. Required items for tubing preparation: nitrogen tank, grade 4.8 (99.998% pure) or
higher; nitrogen regulator (#1652425 recommended for U.S. standard connections); 100% ethanol (fresh for each microcarrier
precipitation); spermidine; plasmid.
Sample Preparation Accessories for Helios Gene Gun RNA-microcarrier complexes using the tubing prep station.
Gold microcarriers, Tefzel tubing, cartridge collection/ Coated tubing is cut into 1.25 cm (0.5") cartridges using the
storage vials, and desiccant pellets are needed for Helios tubing cutter. Sample cartridges can be stored for later use
gene gun sample preparation. The gold microcarriers are at 4°C in cartridge collection/storage vials with a desiccant
available in 0.6, 1.0, and 1.6 µm diameters. Lengths of Tefzel pellet. Additional barrel liners, cartridge holders, and other
tubing (up to 76 cm or 30") are coated with the DNA- or accessories are also available.
Ordering Information
Catalog # Description Price, U.S. $
1652244 GeneShot Control Cartridges, positive control bullets, 12 165.00
1652412 Helium Hose Assembly, with Swagelok quick-connect fittings 925.00
1652413 Helium Regulator, CGA 580 female fitting (U.S. standard), with pressure relief valve; 3,490.00
maximum pressure 2,600 psi
1652414 Low-Pressure Helium Regulator for Helios Gene Gun, maximum pressure 400 psi 3,080.00
1652418 Tubing Prep Station, 100/120 V, includes tubing support cylinder, power cord, O-rings, tubing 2,815.00
prep unit, 12' Nalgene nitrogen regulator hose, two 3/16" barb-to-male Luer-Lok fittings, nitrogen
flowmeter fitting, two 1/8" barb-to-male Luer-Lok fittings, 5/64" hex wrench, 10 ml syringe holder
1652420 Tubing Prep Station, 220/240 V 2,815.00
1652421 Syringe Kit, includes syringe adaptor tubing (silicone, 5', 0.104" ID x 0.192" OD), five 10 ml syringes, 84.00
syringe adaptor fitting, five 1/8" barb-to-female Luer-Lok fittings
1652422 Tubing Cutter, includes tubing cutter unit, 10 razor blades 210.00
1652424 Helios Gene Gun Optimization Kit, includes 0.25 g 0.6 µm gold microcarriers, 0.25 g 1.0 µm gold 2,235.00
microcarriers, 0.25 g 1.6 µm gold microcarriers, cartridge kit
1652440 Cartridge Kit, contains 0.5 g PVP (MW 360,000), 5 cartridge collection/storage vials, 5 desiccant pellets, 210.00
50' Tefzel tubing
1652262 0.6 µm Gold Microcarriers, 0.25 g 625.00
1652263 1.0 µm Gold Microcarriers, 0.25 g 625.00
1652264 1.6 µm Gold Microcarriers, 0.25 g 625.00
1652425 Nitrogen Regulator for U.S. Standard Connections 2,630.00
1652416 O-Rings, 5 12.00
1652417 Barrel Liner, 5 145.00
1652426 Cartridge Holder, white, 5 95.00
1652435 Cartridge Extractor Tool, for removal of discharged cartridge 23.00
1652436 Battery, 9 V 13.00
1652441 Tefzel Tubing, 15 m (50') 190.00
1652475 Helios Diffusion Screens, 5 40.00
1652411 Helios Gene Gun Kit, 100/120 V, includes Helios gene gun, 5 O-rings, 5 barrel liners, Inquire
5 white cartridge holders, cartridge extractor tool, 9 V battery
PDS-1000/He System
The PDS-1000/He system accelerates nucleic acid-coated
gold or tungsten microparticles (0.6–1.6 µm) to velocities
necessary to transfect cells, tissues, or organelles.
The system uses a burst of high-pressure helium gas to
accelerate a plastic macrocarrier disk carrying microparticles
toward target cells. A stopping screen retains the
macrocarrier while allowing the microparticles to penetrate
the target cells. The PDS-1000/He system provides:
■■
A reproducible method for transfecting intact cells in
culture, requiring little manipulation of cells
■■
Transfection of cells with unique growth requirements
that are not amenable to other methods of gene transfer
■■
Interactive training and application guide (CD-ROM, Hepta Adaptor PDS-1000/He System
available separately)
Ordering Information
Catalog # Description Price, U.S. $
1652257* DS-1000/He System, includes helium pressure regulator, solenoid, spacer rods, microcarrier launch
P Inquire
assembly, target shelf, 5 macrocarrier holders, tubing
1652258* PDS-1000/He Hepta System, includes PDS-1000/He system, Hepta adaptor Inquire
1652225 Hepta Adaptor for PDS-1000/He System, includes 5 stopping screens 5,905.00
1652259 Voltage Converter, for 220/240 V line voltage 1,710.00
Accessories
1652278 00 Optimization Kit, includes 0.25 g each of 0.6, 1.0, and 1.6 µm gold microcarriers,
5 2,450.00
100 each of 9 rupture disks, 500 macrocarriers, 500 stopping screens
1652335 Macrocarriers, 500 625.00
1652322 Macrocarrier Holders, 5 235.00
1652326 450 psi Rupture Disks, 100 155.00
1652327 650 psi Rupture Disks, 100 155.00
1652328 900 psi Rupture Disks, 100 155.00
1652329 1,100 psi Rupture Disks, 100 155.00
1652330 1,350 psi Rupture Disks, 100 155.00
1652331 1,550 psi Rupture Disks, 100 155.00
1652332 1,800 psi Rupture Disks, 100 155.00
1652333 2,000 psi Rupture Disks, 100 155.00
1652334 2,200 psi Rupture Disks, 100 155.00
1652336 Stopping Screens, 500 385.00
1652226 Hepta Stopping Screens, 50 375.00
1652262 0.6 µm Gold Microcarriers, 0.25 g 625.00
1652263 1.0 µm Gold Microcarriers, 0.25 g 625.00
1652264 1.6 µm Gold Microcarriers, 0.25 g 625.00
1652266 Tungsten M-10 Microcarriers, ~0.7 µm, 6 g 110.00
1652267 Tungsten M-17 Microcarriers, ~1.1 µm, 6 g 110.00
1652268 Tungsten M-20 Microcarriers, ~1.3 µm, 6 g 110.00
1652269 Tungsten M-25 Microcarriers, ~1.7 µm, 6 g 110.00
* Additional items required for operation of the PDS-1000/He system: helium tank, grade 4.5 (99.995% pure) or higher,
pressurized to 2,600 psi; vacuum source.
Subject Index
Accessories for Helios® Gene Gun, 345
Gene Pulser® Electroporation Buffer, 339
Gene Pulser®/MicroPulser™ Electroporation Cuvettes, 343
Gene Pulser MXcell™ Electroporation Plates, 341
Gene Pulser Xcell™ Electroporation Systems, 340
Helios® Gene Gun System, 345
MicroPulser™ Electroporator, 342
PDS-1000/He™ and Hepta™ Systems, 346
siLentFect™ Lipid Reagent for RNAi, 337
TransFectin™ Lipid Reagent, 338
Digital PCR
Droplet Digital™ PCR Instrument
Several areas of research depend on the ability to study target DNA sequence variations. Digital PCR technology offers
the ability to quantify molecular genetic changes. It is employed by researchers using numerous applications such as copy
number variation, rare mutation detection, gene expression analysis, cell-free DNA quantification, gene-editing validation,
microRNA (miRNA) detection, genetically modified organism (GMO) quantification, biomarker discovery, and viral load
quantification. Bio-Rad’s QX200™ AutoDG™ Droplet Digital™ PCR and QX200 Droplet Digital PCR systems, combined with
our ddPCR™ supermixes, ddPCR assays and kits, and reliable thermal cyclers, offer researchers an easy-to-use, highly
precise, and reproducible digital PCR package.
Learn More about the Technology
Web: bio-rad.com/tech/ddPCR
Ordering Information
Catalog # Description Price, U.S. $
QX200 Droplet Digital PCR System
1864001 QX200 Droplet Digital PCR System, includes droplet generator, droplet reader, laptop computer, Inquire
software, associated component consumables
1864100 QX200 AutoDG Droplet Digital PCR System, includes automated droplet generator, Inquire
droplet reader, laptop computer
1864002 QX200 Droplet Generator, includes droplet generator, 1 box of 24 cartridges, 1 pkg of 24 gaskets, Inquire
2 cartridge holders, 1 power cord
1864101 Automated Droplet Generator, includes automated droplet generator, 1 power cord Inquire
1864003 QX200 Droplet Reader, includes droplet reader, 2 plate holders, 1 USB cable, 1 power cord Inquire
Consumables and Accessories for both QX100 and QX200 ddPCR Systems
1863004 ddPCR Droplet Reader Oil, 2 x 1 L bottles 1,240.00
1863005 Droplet Generation Oil for Probes, 10 x 7 ml bottles 270.00
1864008 DG8 Cartridges for QX100/QX200 Droplet Generator, 1 pkg of 24 cartridges 225.00
1863009 DG8 Gaskets for QX100/QX200 Droplet Generator, 1 pkg of 24 gaskets 58.00
1864007 Droplet Generator Cartridges and Gaskets, 5 pkg of 24 DG8 cartridges, 5 pkg of 24 DG8 gaskets 1,375.00
1863051 DG8 Cartridge Holder 545.00
Consumables and Accessories for QX200 ddPCR System Only
1864005 Droplet Generation Oil for EvaGreen, 2 x 7 ml bottles 58.00
1864006 Droplet Generation Oil for EvaGreen, 10 x 7 ml bottles 270.00
Consumables and Accessories for Automated Droplet Generator Only
1864108 DG32 Automated Droplet Generator Cartridges, 1 pkg of 30, enough for 10 x 96-well ddPCR plates 1,460.00
1864109 DG32 Automated Droplet Generator Cartridges, 1 pkg of 60, enough for 20 x 96-well ddPCR plates 2,600.00
1864110 Automated Droplet Generation Oil for Probes, 140 ml, enough for 20 x 96-well ddPCR plates 575.00
1864112 Automated Droplet Generation Oil for EvaGreen, 140 ml, enough for 20 x 96-well ddPCR plates 575.00
1864120 Pipet Tips for AutoDG System, 1 pkg of 20, enough for 10 x 96-well ddPCR plates 335.00
1864121 Pipet Tips for AutoDG System, 1 pkg of 40, enough for 20 x 96-well ddPCR plates 625.00
1864125 Pipet Tip Waste Bins for AutoDG System, 1 pkg of 10, enough for 10 x 96-well ddPCR plates 31.00
Ordering Information
Catalog # Description Price, U.S. $
1863023 dPCR Supermix for Probes (No dUTP), 2 x 1 ml vials, 2x supermix, for use in nucleic acid
d 175.00
sample preparation with the QX100 or QX200 droplet generator
1863024 ddPCR Supermix for Probes (No dUTP), 5 x 1 ml vials, 2x supermix, for use in nucleic acid 440.00
sample preparation with the QX100 or QX200 droplet generator
1863025 ddPCR Supermix for Probes (No dUTP), 5 x 5 ml vials, 2x supermix, for use in nucleic acid 2,180.00
sample preparation with the QX100 or QX200 droplet generator
See Also New ddPCR™ Residual DNA Quantification Supermix and Kits
C1000 Touch
thermal cycler:
page 357. ddPCR residual DNA quantification supermix and kits
PX1 PCR enable precise quantification of low levels of Chinese
plate sealer:
page 386. hamster ovary (CHO), Escherichia coli, yeast, human, and
mouse DNA for residual host cell DNA monitoring.
■■
nables direct quantification of residual host cell
E
DNA without the need for DNA extraction
■■
Minimizes nonspecific PCR amplification
For More Information
Web: bio-rad.com/digitalpcr
Ordering Information
Catalog # Description Price, U.S. $
1864037 ddPCR Supermix for Residual DNA Quantification, 2 x 1 ml vials, 2x supermix, for use in residual 350.00
DNA detection with the QX100 or QX200 droplet generator
1864038 ddPCR Supermix for Residual DNA Quantification, 5 x 1 ml vials, 2x supermix, for use in residual 875.00
DNA detection with the QX100 or QX200 droplet generator
1864039 ddPCR Supermix for Residual DNA Quantification, 5 x 5 ml vials, 2x supermix, for use in residual 4,360.00
DNA detection with the QX100 or QX200 droplet generator
1864040 ddPCR Supermix for Residual DNA Quantification, 10 x 5 ml vials, 2x supermix, for use in residual 8,705.00
DNA detection with the QX100 or QX200 droplet generator
17000031 ddPCR Residual CHO Host Cell DNA Quantification Kit, includes assay and supermix, for use in Inquire
residual DNA detection with the QX100 or QX200 droplet generator
17000032 ddPCR Residual E. coli Host Cell DNA Quantification Kit, includes assay and supermix, for use in Inquire
residual DNA detection with the QX100 or QX200 droplet generator
Ordering Information
Catalog # Description Price, U.S. $
1863026 ddPCR Supermix for Probes, 2 x 1 ml vials, 2x supermix, for use in sample preparation for 175.00
QX100 or QX200 droplet generator
1863010 ddPCR Supermix for Probes, 5 x 1 ml vials, 2x supermix, for use in sample preparation for 440.00
QX100 or QX200 droplet generator
1863027 ddPCR Supermix for Probes, 5 x 5 ml vials, 2x supermix, for use in sample preparation for 2,180.00
QX100 or QX200 droplet generator
1863028 ddPCR Supermix for Probes, 10 x 5 ml vials, 2x supermix, for use in sample preparation for 4,355.00
QX100 or QX200 droplet generator
Ordering Information
Catalog # Description Price, U.S. $
1864021 One-Step RT-ddPCR Advanced Kit for Probes, 2 x 1 ml, 200 x 20 µl reactions 980.00
1864022 One-Step RT-ddPCR Advanced Kit for Probes, 5 x 1 ml, 500 x 20 µl reactions 2,450.00
Ordering Information
Catalog # Description Price, U.S. $
1864033 QX200 ddPCR EvaGreen Supermix, 2 x 1 ml vials, for use in nucleic acid sample preparation 175.00
with the QX200 droplet generator
1864034 QX200 ddPCR EvaGreen Supermix, 5 x 1 ml vials, for use in nucleic acid sample preparation 450.00
with the QX200 droplet generator
1864035 QX200 ddPCR EvaGreen Supermix, 5 x 5 ml vials, for use in nucleic acid sample preparation 2,180.00
with the QX200 droplet generator
1864036 QX200 ddPCR EvaGreen Supermix, 10 x 5 ml vials, for use in nucleic acid sample preparation 4,355.00
with the QX200 droplet generator
Ordering Information
Catalog # Description Price, U.S. $
1863040 dPCR Library Quantification Kit for Illumina TruSeq, includes 1 vial of primers and probes at
d 880.00
20x concentration, 2x ddPCR supermix for probes (no dUTP)
1863041 ddPCR Library Quantification Kit for Ion Torrent, includes 1 vial of primers and probes at 920.00
20x concentration, 2x ddPCR supermix for probes (no dUTP)
Ordering Information
Visit bio-rad.com/PrimePCR to place an order.
Ordering Information
Catalog # Description Price, U.S. $
1863500 dPCR SMN1 Copy Number Determination Kit, includes assay at 20x concentration,
d 1,875
2x ddPCR supermix for probes (no dUTP), and positive controls
Ordering Information
Catalog # Description Price, U.S. $
1863503 dPCR SMN2 Copy Number Determination Kit, includes assay at 20x concentration,
d 1,875.00
2x ddPCR supermix for probes (no dUTP), and positive controls
ddPCR mutation screening kits are designed for rapid Kit Mutations Detected
screening of several key cancer mutations in a single KRAS G12/13 G12A, G12C, G12D, G12R, G12S, G12V, G13D
reaction with a limit of detection of 0.5% or better. These BRAF V600 V600E, V600K, V600R
kits are ideal for use with DNA extracted from formalin- NRAS Q61 Q61K, Q61L, Q61R , Q61H (183A>C), Q61H (183A>T)
fixed, paraffin-embedded (FFPE) samples, liquid biopsy, NRAS G12 G12A, G12C, G12D, G12R, G12S, G12V
NRAS G12/13 G12A, G12C, G12D, G12S, G12V, G13D, G13R, G13V
fresh/frozen tissue, and samples with low yield or inhibitory KRAS Q61 Q61K, Q61L, Q61R, Q61H (183A>C), Q61H (183A>T)
substances. Assays for each of the mutations detected
by the kit can also be ordered individually. The kits are
recommended for use with fragmented samples such
as FFPE tissue, cell-free DNA, and blood spots.
For More Information
Web: bio-rad.com/digitalpcr
Request or download bulletin: 6632
Ordering Information
Catalog # Description Price, U.S. $
1863506 dPCR KRAS G12/G13 Screening Kit, includes 20x multiplex assay and 2x ddPCR supermix
d 2,080.00
for probes (no dUTP)
12001626 ddPCR KRAS Q61 Screening Kit, includes 20x multiplex assay and 2x ddPCR supermix for Inquire
probes (no dUTP)
12001037 ddPCR BRAF V600 Screening Kit, includes 20x multiplex assay and 2x ddPCR supermix for Inquire
probes (no dUTP)
12001006 ddPCR NRAS Q61 Screening Kit, includes 20x multiplex assay and 2x ddPCR supermix for Inquire
probes (no dUTP)
12001094 ddPCR NRAS G12 Screening Kit, includes 20x multiplex assay and 2x ddPCR supermix for Inquire
probes (no dUTP)
12001627 ddPCR NRAS G12/G13 Screening Kit, includes 20x multiplex assay and 2x ddPCR supermix Inquire
for probes (no dUTP)
Ordering Information
Catalog # Description Price, U.S. $
1841100 1000 Touch Thermal Cycler Chassis, includes USB flash drive, power cord;
C 5,550.00
does not include reaction module
1851148 C1000 Touch Thermal Cycler with Dual 48/48 Fast Reaction Module, includes C1000 Touch 8,195.00
thermal cycler chassis, dual 48/48 fast reaction module, USB flash drive
1851196 C1000 Touch Thermal Cycler with 96-Well Fast Reaction Module, includes C1000 Touch 7,495.00
thermal cycler chassis, 96-well fast reaction module, USB flash drive
1851197 C1000 Touch Thermal Cycler with 96–Deep Well Reaction Module, includes C1000 Touch 7,495.00
thermal cycler chassis, 96–deep well reaction module, USB flash drive
1851138 C1000 Touch Thermal Cycler with 384-Well Reaction Module, includes C1000 Touch 7,495.00
thermal cycler chassis, 384-well reaction module, USB flash drive
Ordering Information
Catalog # Description Price, U.S. $
1842000 S1000 Thermal Cycler Chassis, includes power cord; does not include reaction module 4,500.00
1852148 S1000 Thermal Cycler with Dual 48/48 Fast Reaction Module, includes S1000 thermal cycler 6,895.00
chassis, dual 48/48 fast reaction module
1852196 S1000 Thermal Cycler with 96-Well Fast Reaction Module, includes S1000 thermal cycler chassis, 6,495.00
96-well fast reaction module
1852197 S1000 Thermal Cycler with 96–Deep Well Reaction Module, includes S1000 thermal cycler 6,495.00
chassis, 96–deep well reaction module
1852138 S1000 Thermal Cycler with 384-Well Reaction Module, includes S1000 thermal cycler chassis, 6,495.00
384-well reaction module
Accessories
1848000 USB Cable, for use with C1000, C1000 Touch, and S1000 thermal cyclers 34.00
1849000 ube Frame, supports individual 0.2 ml tubes in the C1000, C1000 Touch, and S1000 dual 48- and 96-well
T 25.00
reaction modules
1849001 Tube Frame, supports individual 0.2 ml tubes in the C1000 Touch and S1000 96–deep well reaction module 25.00
1849010 Touch-Screen Protector, for use with C1000 Touch thermal cycler, 2 30.00
1841001 1000-Series Connectivity Kit, includes mouse, mouse pad, USB key 65.00
Reaction Modules
Reaction Module 96-Well Fast 96–Deep Well Dual 48/48-Well Fast 384-Well
Sample capacity
96 x 0.2 ml tubes or 96 x 0.2 ml tubes, 2 x 48 x 0.2 ml tubes or 1 x 384-well plate
1 x 96-well plate 48 x 0.5 ml tubes, or 2 x 48-well plates
1 x 96-well plate
Maximum ramp rate 5°C/sec 2.5°C/sec 4°C/sec 2.5°C/sec
Temperature range 0–100°C 0–100°C 0 –100°C 0 –100°C
Temperature accuracy ±0.2°C of programmed ±0.2°C of programmed ±0.2°C of programmed ±0.2°C of programmed
target at 90°C target at 90°C target at 90°C target at 90°C
Temperature uniformity ±0.4°C well-to-well within ±0.4°C well-to-well within ±0.4°C well-to-well within ±0.4°C well-to-well within
10 sec of arrival at 90°C 10 sec of arrival at 90°C 10 sec of arrival at 90°C 10 sec of arrival at 90°C
Thermal Gradient (available on all reaction modules)
Gradient range 30–100°C
Temperature differential range 1–24°C
Ordering Information
Catalog # Description Price, U.S. $
1840148 ual 48/48 Fast Reaction Module, independent dual 48-well reaction module, fits C1000, C1000 Touch,
D 2,995.00
and S1000 thermal cyclers, gradient enabled
1840196 96-Well Fast Reaction Module, fits C1000, C1000 Touch, and S1000 thermal cyclers, gradient enabled 2,695.00
1840197 96–Deep Well Reaction Module, fits C1000, C1000 Touch, and S1000 thermal cyclers, gradient enabled 2,795.00
1840138 384-Well Reaction Module, fits C1000, C1000 Touch, and S1000 thermal cyclers, gradient enabled 2,795.00
Ordering Information
Catalog # Description Price, U.S. $
1861096 100 Thermal Cycler, includes 96-well thermal cycler, power cord, tube support ring
T 4,495.00
1862000 Tube Support Ring, extra tube support ring for use with individual tubes in the T100 thermal cycler, 2 25.00
1863000 Touch-Screen Protector, for use with T100 thermal cycler, 2 30.00
Recommended Consumables
1708890 iScript cDNA Synthesis Kit, 25 x 20 μl reactions, includes 5x iScript reaction mix, 145.00
iScript reverse transcriptase, nuclease-free water
1708896 iScript Select cDNA Synthesis Kit, 25 x 20 μl reactions, includes 5x iScript reaction mix, iScript reverse 145.00
transcriptase, oligo(dT), random primer mix, gene specific primer enhancer solution, nuclease-free water
1708870 iTaq DNA Polymerase, 250 U (5 U/μl), includes 10x PCR buffer, 50 mM MgCl2 solution 245.00
1725301 iProof High-Fidelity DNA Polymerase, 100 U (2 U/μl), includes 5x HF buffer, 5x GC buffer, 125.00
50 mM MgCl2 solution, DMSO
HSS9601 Hard-Shell High-Profile 96-Well Semi-Skirted PCR Plates, clear shell, clear well, 25 98.00
MLP9601 Multiplate High-Profile 96-Well Unskirted PCR Plates, clear, 25 105.00
MSB1001 Microseal 'B' Adhesive Seals, optically clear, 100 165.00
TBS1201 0.2 ml 12-Tube Strips without Caps, clear, 100 125.00
TCS1201 Domed 12-Cap Strips, for 0.2 ml PCR tubes and plates, clear, 200 71.00
TWI0201 0.2 ml PCR Tubes with Domed Caps, clear, 1,000 58.00
Feature CFX Connect™ CFX96 Touch™ CFX96 Touch™ Deep Well CFX384 Touch™
Base thermal cycler CFX Connect C1000 Touch C1000 Touch C1000 Touch
Sample capacity 96 wells 96 wells 96 wells 384 wells
Sample volume Up to 50 µl Up to 50 µl Up to 125 µl Up to 30 µl
Light source 3 filtered LEDs 6 filtered LEDs 6 filtered LEDs 5 filtered LEDs
in optics shuttle in optics shuttle in optics shuttle in optics shuttle
Optical detection 3 photodiodes 6 photodiodes 6 photodiodes 5 photodiodes
in optics shuttle in optics shuttle in optics shuttle in optics shuttle
Excitation range 450–535 nm 450–684 nm 450–684 nm 450–650 nm
Detection range* 515–580 nm 515–730 nm 515–730 nm 515–690 nm
Multiplex capability Up to 2 targets Up to 5 targets Up to 5 targets Up to 4 targets
FRET capability • • • •
Maximum ramp rate 5°C/sec 5°C/sec 2.5°C/sec 2.5°C/sec
Gradient capability • • • •
Gradient range 30 –100°C 30 –100°C 30 –100°C 30 –100°C
Maximum gradient span 24°C 24°C 24°C 24°C
CFX qualification plate • • • •
* Refer to system instruction manuals or bulletins 6093, 6096, and 6105 for information about useful detection ranges for specific dyes.
Ordering Information
Catalog # Description Price, U.S. $
1855200 FX Connect Real-Time PCR Detection System, includes CFX Connect thermal cycler chassis,
C Inquire
CFX Connect optical reaction module, CFX Manager software, license for qbase+ software,
communication cable, reagents, consumables
1855201 CFX Connect Real-Time PCR Detection System, includes CFX Connect thermal cycler chassis, Inquire
CFX Connect optical reaction module, CFX Manager software, license for qbase+ software,
communication cable
Accessories
1845098 FX Qualification Plate, 96-well format, for use with CFX96, CFX96 Touch, CFX96 Touch Deep Well, or
C 595.00
CFX Connect system, includes 1 predispensed plate containing supermix, primer mix, nuclease-free water
HSP9601 Hard-Shell Low-Profile 96-Well Skirted PCR Plates, white shell, clear well, 50 235.00
MSB1001 Microseal 'B' Adhesive Seals, optically clear, package of 100 165.00
1709799 Real-Time PCR Applications Guide 22.00
1814000 PX1 PCR Plate Sealer, includes heat sealing instrument, 96-well/384-well plate support block, 3,710.00
sealing frame, power cord
1814030 Optically Clear Heat Seal, package of 100 115.00
Ordering Information
Catalog # Description Price, U.S. $
1845096* FX96 Optical Reaction Module for Real-Time PCR Systems with Starter Package, for use with C1000
C Inquire
Touch thermal cycler chassis, includes CFX Manager software, license for qbase+ software, communication
cable, reagents, consumables
1845097* CFX96 Optical Reaction Module for Real-Time PCR Systems, for use with C1000 Touch thermal cycler Inquire
chassis, includes CFX Manager software, license for qbase+ software, communication cable
1855196 CFX96 Touch Real-Time PCR Detection System with Starter Package, includes C1000 Touch Inquire
thermal cycler chassis, CFX96 optical reaction module, CFX Manager software, license for qbase+
software, communication cable, reagents, consumables
1855195 CFX96 Touch Real-Time PCR Detection System, includes C1000 Touch thermal cycler chassis, Inquire
CFX96 optical reaction module, CFX Manager software, license for qbase+ software, communication cable
Accessories
1845098 FX Qualification Plate, 96-well format, for use with CFX96, CFX96 Touch, CFX96 Touch deep well, or
C 595.00
CFX Connect system, includes 1 predispensed plate containing supermix, primer mix, nuclease-free water
TLS0801 Low-Profile 8-Tube Strips without Caps (0.2 ml), clear, 120 strips (960 tubes) 105.00
TCS0803 Optical Flat 8-Cap Strips, for 0.2 ml PCR tubes and plates, ultraclear, 120 38.00
HSP9601 Hard-Shell Low-Profile 96-Well Skirted PCR Plates, white shell, clear well, 50 235.00
MSB1001 Microseal 'B' Adhesive Seals, optically clear, package of 100 165.00
1709799 Real-Time PCR Applications Guide 22.00
1814000 PX1 PCR Plate Sealer, includes heat sealing instrument, 96-well/384-well plate support block, 3,710.00
sealing frame, power cord
1814030 Optically Clear Heat Seal, package of 100 115.00
* Order to upgrade an existing C1000 or C1000 Touch thermal cycler.
■■
apidly screen expression from a few to hundreds of
R
genes with PrimePCR™ assay panels. Just drag and drop
the run file to start runs with a single click
■■
onitor amplification traces on the touch screen in real
M
time. At run completion, automatically receive the data
file for remote monitoring and data analysis
■■
onserve your samples and reagents with true
C
five-target multiplexing
■■
ave time and reduce costs by optimizing assays in a
S
single run using the thermal gradient
For More Information
Web: bio-rad.com/CFX96DeepWell
Request or download bulletins: 6238 and 6243
Ordering Information
Catalog # Description Price, U.S. $
1844096* FX96 Deep Well Optical Reaction Module for Real-Time PCR Systems with Starter Package,
C Inquire
for use with C1000 Touch thermal cycler chassis, includes CFX Manager software, license for qbase+
software, communication cable, reagents, consumables
1844095* CFX96 Deep Well Optical Reaction Module for Real-Time PCR Systems, for use with C1000 Touch Inquire
thermal cycler chassis, includes CFX Manager software, license for qbase+ software, communication cable
1854096 CFX96 Touch Deep Well Real-Time PCR Detection System with Starter Package, includes C1000 Inquire
Touch thermal cycler chassis, CFX96 deep well optical module, CFX Manager software, license for qbase+
software, communication cable, reagents, consumables
1854095 CFX96 Touch Deep Well Real-Time PCR Detection System, includes C1000 Touch thermal cycler chassis, Inquire
CFX96 deep well optical module, CFX Manager software, license for qbase+ software, communication cable
Accessories
1845098 FX Qualification Plate, 96-well format, for use with CFX96, CFX96 Touch, CFX96 Touch deep well, or
C 595.00
CFX Connect system, includes 1 predispensed plate containing supermix, primer mix, nuclease-free water
TLS0801 Low-Profile 8-Tube Strips without Caps (0.2 ml), clear, 120 strips (960 tubes) 105.00
TCS0803 Optical Flat 8-Cap Strips, for 0.2 ml PCR tubes and plates, ultraclear, 120 38.00
HSP9601 Hard-Shell Low-Profile 96-Well Skirted PCR Plates, white shell, clear well, 50 235.00
MSB1001 Microseal 'B' Adhesive Seals, optically clear, package of 100 165.00
1709799 Real-Time PCR Applications Guide 22.00
1814000 PX1 PCR Plate Sealer, includes heat sealing instrument, 96-well/384-well plate support block, 3,710.00
sealing frame, power cord
1814030 Optically Clear Heat Seal, package of 100 115.00
* Order to upgrade an existing C1000 or C1000 Touch thermal cycler.
Ordering Information
Catalog # Description Price, U.S. $
1845384* CFX384 Optical Reaction Module for Real-Time PCR Systems with Starter Package, for use with C1000 Inquire
Touch thermal cycler chassis, includes CFX Manager software, license for qbase+ software, communication
cable, reagents, consumables
1845385* CFX384 Optical Reaction Module for Real-Time PCR Systems, for use with C1000 Touch thermal cycler Inquire
chassis, includes CFX Manager software, license for qbase+ software, communication cable
1855484 CFX384 Touch Real-Time PCR Detection System with Starter Package, includes C1000 Touch Inquire
thermal cycler chassis, CFX384 optical reaction module, CFX Manager software, license for qbase+
software, communication cable, reagents, consumables
1855485 CFX384 Touch Real-Time PCR Detection System, includes C1000 Touch thermal cycler Inquire
chassis, CFX384 optical reaction module, CFX Manager software, license for qbase+ software,
communication cable
Accessories
1845099 FX Qualification Plate, 384-well format, for use with CFX384 or CFX384 Touch system,
C 835.00
includes 1 predispensed plate containing supermix, primer mix, nuclease-free water
HSP3805 Hard-Shell 384-Well Standard PCR Plates, clear shell, white well, 50 340.00
MSB1001 Microseal 'B' Adhesive Seals, optically clear, package of 100 165.00
1709799 Real-Time PCR Applications Guide 22.00
1814000 PX1 PCR Plate Sealer, includes heat sealing instrument, 96-well/384-well plate support block, 3,710.00
sealing frame, power cord
1814030 Optically Clear Heat Seal, package of 100 115.00
* Order to upgrade an existing C1000 or C1000 Touch thermal cycler.
Ordering Information
Catalog # Description Price, U.S. $
1845075 CFX Automation System II for Real-Time PCR Systems, includes plate handler and Inquire
barcode scanner, mounting plate, automation software; does not include CFX real-time PCR
detection system
CFX Connect™ CFX96 Touch™ CFX96 Touch™ Deep Well CFX384 Touch™
CFX qualification plate • • • •
Ordering Information
Catalog # Description Price, U.S. $
1845098 FX Qualification Plate, 96-well format, for use with CFX96, CFX96 Touch, CFX96 Touch deep well, or
C 595.00
CFX Connect system, includes 1 predispensed plate containing supermix, primer mix, nuclease-free water
1845099 CFX Qualification Plate, 384-well format, for use with CFX384 or CFX384 Touch system, includes 1 835.00
predispensed plate containing supermix, primer mix, nuclease-free water
Ordering Information
Catalog # Description Price, U.S. $
1845000 CFX Manager Software, includes installation CD, quick guides, instruction manual 2,910.00
CFX Manager software, Security Edition provides important each time a file is opened with automatic file checking and
tools for compliance with U.S. FDA 21 CFR Part 11 allows read-only information displayed in the time- and
regulations. The Security Edition requires a valid Windows XP, date-stamped audit trail to be viewed only while the data
Windows 7, or Windows 8 username and password for file of interest is open.
login. The software requires a hardware protection key For More Information
to be attached to a USB port on the computer, uses file Web: bio-rad.com/cfx-manager-software-security-edition
encryption to ensure files cannot be opened or edited using Request or download bulletin: 5690
other programs, and allows multiple electronic signatures.
The software ensures that integrity and validity are checked
Ordering Information
Catalog # Description Price, U.S. $
1845001 CFX Manager Software, Security Edition, includes 1 user license, installation CD, HASP HL key Inquire
1845005 CFX Manager Software, Security Edition, includes 5 user licenses, 5 installation CDs, 5 HASP HL keys Inquire
1845010 CFX Manager Software, Security Edition, includes 10 user licenses, 10 installation CDs, 10 HASP HL keys Inquire
CFX Manager software Chinese and Russian Editions work software: a HASP hardware license (HL)-based key must
with the regional settings of the Windows XP, Windows 7, be attached to a USB port on the computer to use the
and Windows 8 operating systems to provide localized, software in regional language mode.
language-specific environments. The Chinese and Russian For More Information
Editions also provide hardware protection to CFX Manager Web: bio-rad.com/cfx-manager-software-chinese-edition;
bio-rad.com/cfx-manager-software-russian-edition
Ordering Information
Catalog # Description Price, U.S. $
1845008 CFX Manager Software, Chinese Edition, includes 3 user licenses, installation CD, 3 HASP HL keys 2,925.00
1845028 CFX Manager Software, Russian Edition, includes 3 user licenses, installation CD, 3 HASP HL keys 3,145.00
Ordering Information
Catalog # Description Price, U.S. $
1845025 Precision Melt Analysis Software, includes 2 user licenses, installation CD, 2 HASP HL keys, melt calibration kit 4,100.00
1845015 Precision Melt Analysis Software Only, includes 2 user licenses, installation CD, 2 HASP HL keys 3,560.00
1845020 Melt Calibration Kit, includes melt calibration DNA standard, melt primers, precision melt supermix 545.00
■■
redesigned probe assays — genome-wide 5'
P
nuclease probe assays for gene expression analysis
available in 500, 1,000, and 2,500 reactions
■■
ustom assays — order your primer and probe
C
sequences of interest, available in 200, 500, 1,000,
and 2,500 reactions
■■
ustom PCR plates — custom-configured 96- and
C
384-well PCR plates
■■
athway and collection PCR arrays — predesigned
P
96- and 384-well PCR pathway and collection panels
■■
NA templates — synthetic DNA templates can
D
be used as a positive control for the corresponding
gene-specific assay
■■
Experimental controls — control assays are available
for reverse transcription, RNA quality, genomic DNA
Wide Range of Pathway and Collection PCR Arrays contamination, and PCR performance
Predesigned plates are available for signaling and disease ■■
eference gene assays — commonly used reference
R
pathways to help identify and investigate key targets in a
gene assays are available to normalize for variation in the
biological pathway of interest. Predesigned plates can be
amount of input mRNA among samples
modified to include user-selected assays.
■■
PreAmp assays — assays for the unbiased, target-
Customizable 96- and 384-Well Plate Formats
specific preamplification of up to 100 gene targets in a
Easy-to-use custom plate configurator allows users to lay
single reaction
out assays on a plate exactly as they choose.
Fully Integrated with CFX Manager™ Software PrimePCR assays are also available for Droplet Digital™
Full integration with CFX real-time PCR systems and data PCR. Please see page 355 for more information.
analysis software streamlines data collection and analysis. For More Information
Web: bio-rad.com/PrimePCR
The PrimePCR qPCR product family includes: Download bulletins: 6262, 6263, and 6290
■■
redesigned primer assays — genome-wide assays
P
for SYBR® Green gene expression analysis available in
200, 1,000, and 2,500 reactions
• Recommended for use as is; u ROX reference setting must be turned “off”; s BSA must be added according to instrument specifications
Use this selection guide to choose an appropriate reagent for your application.
SsoAdvanced™
Feature Universal Supermixes iTaq™ Universal Supermixes iQ™ Supermixes
Sensitive detection of low-level target genes ••• ••• ••
Efficiency with difficult target sequences ••• •• ••
Priming specificity ••• •• •
Tolerance to PCR inhibitors ••• — —
Broad range of reaction conditions ••• •• •
Fast cycling ••• ••• •
Instrument compatibility Any instrument Any instrument Select instruments
• •• Excellent •• Good • Fair
■■
Increase your qPCR sensitivity and efficiency of for challenging applications, such as direct qPCR, without the need for
detection from compromised samples — Sso7d fusion sample preparation.
polymerase has increased resistance to a wide variety
Applications and Uses of SsoAdvanced™ Universal
of PCR inhibitors, providing better sensitivity and overall
SYBR® Green Supermix
performance
Real-time PCR
■■
■■
chieve superior real-time PCR results under any
A Gene expression analysis
■■
■■
ecrease time to results without compromising
D Pathogen detection
■■
qPCR data quality — Sso7d fusion polymerase and Characterization of genetically modified organisms (GMO)
■■
Ordering Information
Catalog # Description Price, U.S. $
1725270 SsoAdvanced Universal SYBR Green Supermix, 2 ml (2 x 1 ml vials), 200 x 20 μl reactions 160.00
1725271 SsoAdvanced Universal SYBR Green Supermix, 5 ml (5 x 1 ml vials), 500 x 20 μl reactions 355.00
1725272 SsoAdvanced Universal SYBR Green Supermix, 10 ml (10 x 1 ml vials), 1,000 x 20 μl reactions 680.00
1725274 SsoAdvanced Universal SYBR Green Supermix, 25 ml (5 x 5 ml vials), 2,500 x 20 μl reactions 1,570.00
1725275 SsoAdvanced Universal SYBR Green Supermix, 50 ml (10 x 5 ml vials), 5,000 x 20 μl reactions 2,985.00
■■
arry out high-performance singleplex and multiplex
C Genotyping (allelic discrimination)
■■
data precision and allowing cost and time savings when Genetic profiling
■■
Ordering Information
Catalog # Description Price, U.S. $
1725280 SsoAdvanced Universal Probes Supermix, 2 ml (2 x 1 ml vials), 200 x 20 μl reactions 155.00
1725281 SsoAdvanced Universal Probes Supermix, 5 ml (5 x 1 ml vials), 500 x 20 μl reactions 345.00
1725282 SsoAdvanced Universal Probes Supermix, 10 ml (10 x 1 ml vials), 1,000 x 20 μl reactions 665.00
1725284 SsoAdvanced Universal Probes Supermix, 25 ml (5 x 5 ml vials), 2,500 x 20 μl reactions 1,550.00
1725285 SsoAdvanced Universal Probes Supermix, 50 ml (10 x 5 ml vials), 5,000 x 20 μl reactions 2,960.00
Ordering Information
Catalog # Description Price, U.S. $
1725120 iTaq Universal SYBR Green Supermix, 2 ml (2 x 1 ml vials), 200 x 20 μl reactions 135.00
1725121 iTaq Universal SYBR Green Supermix, 5 ml (5 x 1 ml vials), 500 x 20 μl reactions 285.00
1725122 iTaq Universal SYBR Green Supermix, 10 ml (10 x 1 ml vials), 1,000 x 20 μl reactions 545.00
1725124 iTaq Universal SYBR Green Supermix, 25 ml (5 x 5 ml vials), 2,500 x 20 μl reactions 1,265.00
1725125 iTaq Universal SYBR Green Supermix, 50 ml (10 x 5 ml vials), 5,000 x 20 μl reactions 2,400.00
1725130 iTaq Universal Probes Supermix, 2 ml (2 x 1 ml vials), 200 x 20 μl reactions 135.00
1725131 iTaq Universal Probes Supermix, 5 ml (5 x 1 ml vials), 500 x 20 μl reactions 285.00
1725132 iTaq Universal Probes Supermix, 10 ml (10 x 1 ml vials), 1,000 x 20 μl reactions 545.00
1725134 iTaq Universal Probes Supermix, 25 ml (5 x 5 ml vials), 2,500 x 20 μl reactions 1,265.00
1725135 iTaq Universal Probes Supermix, 50 ml (10 x 5 ml vials), 5,000 x 20 μl reactions 2,400.00
Ordering Information
Catalog # Description Price, U.S. $
1725016 SsoAdvanced Universal Inhibitor-Tolerant SYBR Green Supermix, 2 ml (2 x 1 ml vials), 190.00
200 x 20 μl reactions
1725017 SsoAdvanced Universal Inhibitor-Tolerant SYBR Green Supermix, 5 ml (1 x 5 ml vial), 430.00
500 x 20 μl reactions
1725018 SsoAdvanced Universal Inhibitor-Tolerant SYBR Green Supermix, 10 ml (2 x 5 ml vials), 820.00
1,000 x 20 μl reactions
iQ™ Supermixes
Ordering Information
Catalog # Description Price, U.S. $
1708880 iQ SYBR Green Supermix, 2.5 ml (2 x 1.25 ml vials), 100 x 50 μl reactions 215.00
1708882 iQ SYBR Green Supermix, 12.5 ml (10 x 1.25 ml vials), 500 x 50 μl reactions 940.00
1708884 iQ SYBR Green Supermix, 25 ml (20 x 1.25 ml vials), 1,000 x 50 μl reactions 1,815.00
1708885 iQ SYBR Green Supermix, 50 ml (50 ml bottle), 2,000 x 50 μl reactions 3,560.00
1708886 iQ SYBR Green Supermix, 25 ml (5 x 5 ml vials), 1,000 x 50 μl reactions 1,685.00
1708887 iQ SYBR Green Supermix, 50 ml (10 x 5 ml vials), 2,000 x 50 μl reactions 3,125.00
1708860 iQ Supermix, 2.5 ml (2 x 1.25 ml vials), 100 x 50 μl reactions 210.00
1708862 iQ Supermix, 12.5 ml (10 x 1.25 ml vials), 500 x 50 μl reactions 915.00
1708864 iQ Supermix, 25 ml (20 x 1.25 ml vials), 1,000 x 50 μl reactions 1,775.00
1725848 iQ Multiplex Powermix, 1.25 ml (1 x 1.25 ml vial), 50 x 50 μl reactions 175.00
1725849 iQ Multiplex Powermix, 5 ml (4 x 1.25 ml vials), 200 x 50 μl reactions 625.00
reference dye in this reaction mix enables ROX Gene expression analysis
■■
■■
chieve superior real-time PCR results under most
A
Instrument Compatibility
conditions — robust formulation delivers consistent
The iTaq™ universal SYBR® Green one-step kit is
performance in fast cycling across a broad range
compatible with Bio-Rad’s and all other commercially
of reaction conditions, primer concentrations, and
available real-time qPCR systems.
temperature ranges
For More Information
■■
Obtain better results with PrimePCR™ assays — Web: bio-rad.com/supermixes
real-time PCR assays are expertly designed and wet-lab View the Universal Real-Time PCR Reagents Web App:
validated to ensure optimal assay performance bio-rad.com/App/UniversalSupermixes
Ordering Information
Catalog # Description Price, U.S. $
iTaq Universal SYBR Green One-Step Kit
1725150 iTaq Universal SYBR Green One-Step Kit, 100 reactions, 1 ml (1 x 1 ml vial), includes 25 μl RT (1 vial), 200.00
nuclease-free water (1 vial)
1725151 iTaq Universal SYBR Green One-Step Kit, 500 reactions, 5 ml (5 x 1 ml vials), includes 125 μl RT (1 vial), 740.00
nuclease-free water (1 vial)
Multiplexing
■■
■■
arry out high-throughput real-time PCR screening and
C
Absolute quantification
■■
Ordering Information
Catalog # Description Price, U.S. $
iTaq Universal Probes One-Step Kit
1725140 iTaq Universal Probes One-Step Kit, 100 reactions, 1 ml (1 x 1 ml vial), includes 50 μl RT (1 x 50 μl vial), 200.00
nuclease-free water (1 vial)
1725141 iTaq Universal Probes One-Step Kit, 500 reactions, 5 ml (5 x 1 ml vials), includes 250 μl RT (2 x 125 μl vials), 740.00
nuclease-free water (1 vial)
■■
nalyze up to 400 targets — when the number of
A –0.75
■■
equires as little as 100 pg DNA — enables analysis of
R –3.00
any precious or limited sample, such as stem cells, laser 0 20 40 60 80 100
capture microdissections, and formalin-fixed paraffin- qPCR assay number
embedded tissues
Minimal bias observed during preamplification of 100 targets.
■■
ptimized for use with PrimePCR — use PrimePCR
O Preamplification was performed on a cDNA sample for ten cycles using
PreAmp Assays before downstream analysis with SsoAdvanced PreAmp Supermix and 100 PrimePCR PreAmp Assays.
PrimePCR Gene Expression Assay The preamplified cDNA was diluted and used in qPCR analysis alongside
cDNA that was not subject to preamplification. The bias quantification
For More Information (BQ) score shows the difference in the results (quantification cycle [Cq]
Web: bio-rad.com/PreAmp value) for the samples with and without preamplification. BQ scores close
Request or download bulletin: 6576 to zero indicate a lack of preamplification bias.
Ordering Information
Catalog # Description Price, U.S. $
1725160 SsoAdvanced PreAmp Supermix, 1.25 ml (1 x 1.25 ml vial), 50 x 50 μl reactions 645.00
Ordering Information
Catalog # Description Price, U.S. $
1725080 SingleShot Cell Lysis Kit, 100 x 50 μl reactions 465.00
1725081 SingleShot Cell Lysis Kit, 500 x 50 μl reactions 2,060.00
One-Step Kits
1725070 SingleShot Probes One-Step Kit, 100 x 50 μl reactions 1,135.00
1725095 SingleShot SYBR Green One-Step Kit, 100 x 50 μl reactions 1,135.00
Two-Step Kits
1725090 SingleShot Probes Kit, 100 x 50 μl reactions 1,135.00
1725085 SingleShot SYBR Green Kit, 100 x 50 μl reactions 1,135.00
■■
ensitive and specific discrimination of class I–IV SNPs
S A B
0.02 0.02
across a broad range of amplicons
Difference RFU
0.00
Difference RFU
0.00
■■
Ideal solution for insertions or deletions >6 base pairs –0.02 –0.02
–0.04 –0.04
■■
De novo SNP discovery
–0.06 –0.06
■■
ccurate quantification of CpG methylation status for
A –0.08 –0.08
epigenetic studies
75 76 77 78 79 80 75 76 77 78 79 80
■■
Ideal for mutation screening of small mutations or when Temperature, °C Temperature, °C
Ordering Information
Catalog # Description Price, U.S. $
1725110 recision Melt Supermix, 2 ml (2 x 1 ml vials), 200 x 20 μl reactions
P 160.00
1725112 Precision Melt Supermix, 10 ml (10 x 1 ml vials), 1,000 x 20 μl reactions 680.00
RECOMMENDED Minimum NEW! Effective Genomic Maximum Input RNA Flexible Reliable
Feature Reaction Setup Time DNA Removal before RT for High cDNA Yields Priming Options Value Solution
Product iScript Reverse iScript gDNA Clear iScript Advanced iScript Select cDNA iScript cDNA
Transcription cDNA Synthesis Kit cDNA Synthesis Kit Synthesis Kit Synthesis Kit
Supermix for RT-qPCR for RT-qPCR
Applications ■■
Gene expression ■■
Gene expression ■■
Gene expression ■■
Gene expression ■■
Gene expression
■■
RNA quantification ■■
RNA quantification ■■
RNA quantification ■■
Cloning ■■
RNA quantification
■■
RNA quantification
Total input RNA 1 µg–1 pg 1 µg –1 pg 7.5 µg–100 fg 1 µg–1 pg 1 µg–100 fg
Format 1 tubes 3 tube 2 tubes 5 tubes 2 tubes
Kit contents ■■
5x iScript RT Supermix ■■
5x iScript RT Supermix ■■
iScript Reverse ■■
iScript Reverse ■■
iScript Reverse
■■
No-RT control supermix ■■
No-RT control supermix Transcriptase Transcriptase Transcriptase
■■
DNase ■■
5x iScript Advanced ■■
5x iScript ■■
5x iScript
■■
DNase buffer Reaction Mix Reaction Mix Reaction Mix
■■
3 priming options
Time to 26 min 36 min 21 min 40 – 90 min 26 min
produce cDNA
RNase H+ High High High High High
activity
Ordering Information
Catalog # Description Price, U.S. $
iScript Advanced cDNA Synthesis Kit for RT-qPCR
1725037 iScript Advanced cDNA Synthesis Kit for RT-qPCR, 25 x 20 μl reactions, includes 100 μl 5x iScript 185.00
advanced reaction mix, 25 μl iScript advanced reverse transcriptase, and nuclease-free water
1725038 iScript Advanced cDNA Synthesis Kit for RT-qPCR, 100 x 20 μl reactions, includes 400 μl 5x iScript 580.00
advanced reaction mix, 100 μl iScript advanced reverse transcriptase, and nuclease-free water
iScript Reverse Transcription Supermix for RT-qPCR
1708840 iScript Reverse Transcription Supermix for RT-qPCR, 25 x 20 μl reactions, includes 100 μl 5x iScript RT 160.00
supermix, 200 μl 5x iScript RT supermix no-RT control (50 reactions), and nuclease-free water
1708841 iScript Reverse Transcription Supermix for RT-qPCR, 100 x 20 μl reactions, includes 400 μl 5x iScript RT 485.00
supermix, 200 μl 5x iScript RT supermix no-RT control (50 reactions), and nuclease-free water
iScript cDNA Synthesis Kit
1708890 iScript cDNA Synthesis Kit, 25 x 20 μl reactions, includes 100 µl 5x iScript reaction mix, 145.00
25 µl iScript reverse transcriptase, and nuclease-free water
1708891 iScript DNA Synthesis Kit, 100 x 20 μl reactions, includes 400 µl 5x iScript reaction mix, 425.00
100 µl iScript reverse transcriptase, and nuclease-free water
iScript Select cDNA Synthesis Kit
1708896 iScript Select cDNA Synthesis Kit, 25 x 20 μl reactions, includes 400 µl iScript select reaction mix, 145.00
25 µl iScript reverse transcriptase, 200 µl oligo(dT) mix, 200 µl random primer mix, 200 µl gene-specific primer
enhancer solution, and nuclease-free water
1708897 iScript Select cDNA Synthesis Kit, 100 x 20 μl reactions, includes 400 µl iScript Select reaction mix, 450.00
100 µl iScript reverse transcriptase, 200 µl oligo(dT) mix, 200 µl random primer mix, 200 µl gene-specific primer
enhancer, and nuclease-free water
iScript gDNA Clear cDNA Synthesis Kit
1725034 iScript gDNA Clear cDNA Synthesis Kit, 25 x 20 μl reactions, includes 100 µl 5x iScript RT supermix, 200 µl 170.00
5x iScript no-RT control supermix, 12.5 µl iScript DNase, 150 µl iScript DNase buffer, 1.5 ml nuclease free water
1725035 iScript gDNA Clear cDNA Synthesis Kit, 100 x 20 μl reactions, includes 400 µl 5x iScript RT supermix, 400 µl 505.00
5x iScript no-RT control supermix, 50 µl iScript DNase, 150 µl iScript DNase buffer, 1.5 ml nuclease free water
Ordering Information
Catalog # Description Price, U.S. $
iProof High-Fidelity DNA Polymerase, Master Mixes, and Buffers
1725300 iProof High-Fidelity DNA Polymerase, 2 U/µl, 20 U, includes 5x reaction buffers, 24.00
MgCl2 solution, DMSO
1725301 iProof High-Fidelity DNA Polymerase, 2 U/µl, 100 U 125.00
1725302 iProof High-Fidelity DNA Polymerase, 2 U/µl, 500 U 500.00
1725330 iProof High-Fidelity PCR Kit, 2 U/µl, 50 U, includes 5x reaction buffers, MgCl2 solution, DMSO, dNTPs, 75.00
l DNA, 1.3 and 10 kb primers, DNA standard
1725331 iProof High-Fidelity PCR Kit, 2 U/µl, 200 U, includes 5x reaction buffers, MgCl2 solution, DMSO, dNTPs, 265.00
l DNA, 1.3 and 10 kb primers, DNA standard
1725310 iProof HF Master Mix, 2.5 ml, 100 x 50 µl reactions (for highest fidelity with most templates) 195.00
1725320 iProof GC Master Mix, 2.5 ml, 100 x 50 µl reactions (for GC-rich templates) 285.00
1725391 5x iProof HF Buffer, 1.5 ml (for highest fidelity with most templates) 23.00
1725392 5x iProof GC Buffer, 1.5 ml (for GC-rich templates) 23.00
1725393 5x iProof HPLC HF Buffer, 1.5 ml 23.00
1725394 5x iProof HPLC GC Buffer, 1.5 ml 23.00
dNTP Mix
1708874 dNTP Mix, 200 µl premixed solution, contains 10 mM each dNTP (dATP, dCTP, dGTP, dTTP) 130.00
iTaq DNA Polymerase
1708870 iTaq DNA Polymerase, 5 U/µl, includes 250 U polymerase, 1.25 ml 10x PCR buffer 245.00
1708875 iTaq DNA Polymerase, 5 U/µl, includes 5,000 U polymerase, 25 ml 10x PCR buffer, 3,690.00
25 ml 50 mM MgCl2 solution
MgCl2
1708872 MgCl2 Solution, 50 mM, 1.25 ml 26.00
Ordering Information
Catalog # Description Price, U.S. $
ROX Passive Reference Dye
1725858 ROX Passive Reference Dye, 0.5 ml 47.00
Precision Blue Real-Time PCR Dye
1725555 Precision Blue Real-Time PCR Dye, 55 μl, 200x concentrated blue visualization 25.00
dye for use with real-time PCR systems
Ordering Information
Catalog # Description Price, U.S. $
PX1 PCR Plate Sealer
1814000 PX1 PCR Plate Sealer, includes heat sealing instrument, 96-well/384-well plate 3,710.00
support block, sealing frame, power cord
Accessories
1814080 Sealing Frame, extra sealing frame for use with PX1 PCR plate sealer, 1 64.00
1814085 Plate Support Block, extra plate support block for use with PX1 PCR plate sealer, 1 280.00
Heat Seals for PX1 PCR Plate Sealer
1814030 Optically Clear Heat Seal, package of 100 115.00
1814035 Permanent Clear Heat Seal, package of 100 115.00
1814040 Pierceable Foil Heat Seal, package of 100 87.00
1814045 Peelable Foil Heat Seal, package of 100 87.00
For more information about the heat seals that are compatible with the PX1 PCR plate sealer, see page 397.
optical applications.
Flat and Domed Cap Strips for PCR Tubes
and PCR Plates
These cap strips provide extremely tight sealing of all Bio-Rad
PCR tubes and plates during thermal cycling and cold TCS-0801
storage. Flat cap strips feature ultraclear upper surfaces,
which are ideal for fluorescence applications. Average light
transmittance is 1.7-fold higher than with standard-clarity
domed cap strips. Flat caps are available in strips of 8 and
domed caps are available in strips of 8 or 12. Use of a
capping tool is recommended for proper sealing of caps
on tubes or plates.
For More Information
Web: bio-rad.com/pcrplastics
Ordering Information
Catalog # Description Price, U.S. $
Individual PCR Tubes with Attached Caps (0.2 ml)
TFI0201 PCR Tubes with Flat Caps (0.2 ml), clear, 1,000 58.00
TWI0201 PCR Tubes with Domed Caps (0.2 ml), clear, 1,000 58.00
Individual PCR Tubes without Caps (0.2 ml)
TBI0201 PCR Tubes without Caps (0.2 ml), clear, 1,000 59.00
Individual PCR Tubes with Attached Caps (0.5 ml)
TBI0501 PCR Tubes with Flat Caps (0.5 ml), clear, 1,000 (2 bags of 500) 54.00
TBI0502 PCR Tubes with Flat Caps (0.5 ml), clear, 800 (8 bags of 100) 43.00
High-Profile Tube Strips without Caps (0.2 ml)
TBS0201 8-Tube Strips without Caps, clear, 125 strips (1,000 PCR tubes) 98.00
TBS1201 12-Tube Strips without Caps, clear, 100 strips (1,200 PCR tubes) 125.00
Low-Profile 8-Tube Strips without Caps (0.2 ml)
TLS0801 Low-Profile 8-Tube Strips without Caps, clear, 120 strips (960 PCR tubes) 105.00
TLS0851 Low-Profile 8-Tube Strips without Caps, white, 120 strips (960 PCR tubes) 110.00
Domed Cap Strips
TCS0801 Domed 8-Cap Strips, for PCR tubes and plates, clear, 130 38.00
TCS1201 Domed 12-Cap Strips, for PCR tubes and plates, clear, 200 71.00
Optical Flat Cap Strips
TCS0803 Optical Flat 8-Cap Strips, for PCR tubes and plates, ultraclear, 120 38.00
High-Profile Polypropylene Tube Strips with Domed Cap Strips (0.2 ml)
TBC0802 8-Tube Strips and Domed Cap Strips, clear, 20 bags of 12 x 8-tube strips and 12 x 8-cap strips 215.00
(1,920 PCR tubes and caps)
TBC1202 12-Tube Strips and Domed Cap Strips, clear, 20 bags of 8 x 12-tube strips and 8 x 12-cap strips 195.00
(1,920 PCR tubes and caps)
Ordering Information
Catalog # Description Price, U.S. $
TRC9601 PCR Tube Racks, ANSI/SBS standard, white, 10 39.00
TRC0501 96-Place Racks, with covers, for PCR tubes and unskirted and semi-skirted microplates, assorted colors, 5 40.00
ECT1000 Easy Cap Tool, ensures tight seal for 0.2 ml PCR tubes or 96-well microplates 16.00
ECT2000 Strip Cap Tool, for sealing 8- and 12-cap strips on PCR plates or tubes 41.00
PCR Plates
Ordering Information
Catalog # Description Price, U.S. $
MLP4801 Multiplate High-Profile 48-Well Unskirted PCR Plates, clear, 50 plates 155.00
MLL4801 Multiplate Low-Profile 48-Well Unskirted PCR Plates, clear, 50 plates 125.00
MLL4851 Multiplate Low-Profile 48-Well Unskirted PCR Plates, white, 50 plates 140.00
■■
ow-profile (16.06 mm) wells optimized for low-volume
L
reactions and fast PCR
■■
Full skirt for robotic handling and labeling surface
■■
ootprint and well spacing that match ANSI/SBS
F
standard dimensions Hard-Shell High-Profile 96-Well
■■
Barcoded plates are available Semi-Skirted PCR Plate
Ordering Information
Description Clear Wells Price, U.S. $ White Wells Price, U.S. $ Black Wells Price, U.S. $
Hard-Shell Low-Profile 96-Well Skirted PCR Plates
White shell, 50 plates HSP9601 235.00 HSP9655 255.00 —
Red shell, 50 plates HSP9611 235.00 — —
Yellow shell, 50 plates HSP9621 235.00 — —
Blue shell, 50 plates HSP9631 235.00 HSP9635 255.00 —
Green shell, 50 plates HSP9641 235.00 HSP9645 255.00 —
Black shell, 50 plates HSP9661 235.00 HSP9665 255.00 HSP9666 255.00
White shell, barcoded, 50 plates HSP9901 245.00 HSP9955 260.00 —
White shell, bulk pack of 400 plates HSP9601B 1,570.00 — —
Hard-Shell High-Profile 96-Well Semi-Skirted PCR Plates, 25 plates
Clear shell HSS9601 98.00 — —
Green shell HSS9641 99.00 — —
Black shell — HSS9665 115.00 —
Clear shell, barcoded HSS9901 110.00 — —
Hard-Shell Low-Profile 96-Well Semi-Skirted PCR Plates, 25 plates
Clear shell HSL9601 110.00 HSL9605 110.00 —
Green shell HSL9641 110.00 HSL9645 110.00 —
Clear shell, barcoded HSL9901 110.00 HSL9905 110.00 —
Ordering Information
Catalog # Description Price, U.S. $
Multiplate High-Profile 96-Well Unskirted PCR Plates
MLP9601 Multiplate High-Profile 96-Well Unskirted PCR Plates, clear, 25 plates 105.00
MLP9651 Multiplate High-Profile 96-Well Unskirted PCR Plates, white, 25 plates 120.00
MLP9631 Multiplate High-Profile 96-Well Unskirted PCR Plates, blue, 25 plates 115.00
Multiplate Low-Profile 96-Well Unskirted PCR Plates
MLL9601 Multiplate Low-Profile 96-Well Unskirted PCR Plates, clear, 25 plates 105.00
MLL9651 Multiplate Low-Profile 96-Well Unskirted PCR Plates, white, 25 plates 120.00
Ordering Information
Catalog # Description Price, U.S. $
2239441 iQ High-Profile 96-Well Semi-Skirted PCR Plates, 25 plates 215.00
Ordering Information
Catalog # Description Price, U.S. $
Microseal High-Profile 96-Well Semi-Skirted PCR Plates
MSS9601 Microseal High-Profile 96-Well Semi-Skirted PCR Plates, clear, 25 plates 105.00
Microseal Low-Profile 96-Well Skirted PCR Plates
MSP9601 Microseal Low-Profile 96-Well Skirted PCR Plates, clear, 50 plates 235.00
MSP9605 Microseal Low-Profile 96-Well Skirted PCR Plates, barcoded, clear, 50 plates 245.00
Ordering Information
Description Clear Wells Price, U.S. $ White Wells Price, U.S. $ Black Wells Price, U.S. $
Hard-Shell 384-Well Standard PCR Plates
Clear shell, 50 plates HSP3801 280.00 HSP3805 340.00 —
Red shell, 50 plates HSP3811 280.00 — —
Yellow shell, 50 plates HSP3821 280.00 — —
Blue shell, 50 plates HSP3831 280.00 — —
Green shell, 50 plates HSP3841 280.00 — —
Black shell, 50 plates — HSP3865 335.00 HSP3866 335.00
Clear shell, barcoded, 50 plates HSP3901 300.00 HSP3905 360.00 —
Clear shell, bulk pack of 500 plates HSP3801B 2,740.00 — —
Ordering Information
Catalog # Description Price, U.S. $
HSR4805 ard-Shell 384-Well 480 PCR Plates, clear shell/white well PCR plate for Roche LightCycler 480 real-time
H 295.00
PCR system, barcoded, rigid 2-component design, 50 plates
HSR4805K Hard-Shell 384-Well 480 PCR Plates Kit, for use with Roche LightCycler 480 real-time PCR system, 705.00
includes 100 barcoded clear shell/white well PCR plates (2 packs of #HSR-4805) and 100 Microseal 'C'
optical seals (#MSC-1001)
HSR4801 Hard-Shell 384-Well 480 PCR Plates, clear shell/clear well PCR plate for Roche LightCycler 480 real-time 295.00
PCR system, barcoded, rigid 2-component design, 50 plates
HSR4801K Hard-Shell 384-Well 480 PCR Plates Kit, for use with Roche LightCycler 480 real-time PCR system, 705.00
includes 100 barcoded clear shell/clear well PCR plates (2 packs of #HSR-4801) and 100 Microseal 'C'
optical seals (#MSC-1001)
Microseal 384-well skirted PCR plates feature single- For More Information
component construction and are ideal for high-throughput Web: bio-rad.com/384wellplates
thermal cycling applications.
■■
Barcoded plates are available
■■
ootprint and well spacing that match ANSI/SBS
F
standard dimensions
Ordering Information
Description 50 Plates Price, U.S. $ Barcoded, 50 Plates Price, U.S. $
Microseal 384-Well Skirted PCR Plates
Clear MSP3842 275.00 MSP3846 290.00
White MSP3852 290.00 —
Black MSP3862 290.00 —
PCR Seals
Effective sealing is essential for PCR and qPCR reactions. Besides cap strips (page 389), Bio-Rad offers many sealing
options to fit your needs.
sequencing (ABI 3700 DNA analyzer) and sample storage Microseal 'A' Film
■■
cts as a barrier against evaporation in extreme
A
temperatures (–80 to 105°C)
■■
Pierceable foil for easy sample retrieval
Ordering Information
Catalog # Description Price, U.S. $
MSC1001 Microseal 'C' Optical Seals, 100 pressure-sensitive adhesive seals 215.00
MSA5001 Microseal 'A' Film, 50 seals 90.00
MSB1001 Microseal 'B' Adhesive Seals, 100 optically clear seals 165.00
MSF1001 Microseal 'F' Foil, 100 adhesive seals 105.00
MSR0001 Sealing Roller, for sealing PCR plates with films 22.00
ADR3296 Optical Compression Pad, for improved film sealing of 96-well plates in DNA Engine Opticon 2 22.00
and Chromo4 systems
ADR5001 Pressure Pad, uniformly distributes lid pressure for sealing film 16.00
MSO1001 Optical Film Sealing Kit, for 96-well plates, includes optical compression pad, 170.00
100 Microseal 'B' clear adhesive seals
2239442 96-Well PCR Plate Sealing Mats, 5 50.00
Ordering Information
Catalog # Description Price, U.S. $
1814030 Optically Clear Heat Seal, package of 100 115.00
1814035 Permanent Clear Heat Seal, package of 100 115.00
1814040 Pierceable Foil Heat Seal, package of 100 87.00
1814045 Peelable Foil Heat Seal, package of 100 87.00
Microseal® 'P' and 'P+' Sealing Pads Auto-Sealing Lids for PCR Plates
These reusable sealing pads are designed to adhere to a These lids are reusable automation-friendly sealers that
motorized heated lid. Use 'P' pads with Power Bonnet™ prevent evaporation and contamination during reaction
lids, and 'P+' pads with Moto Alpha™ units. Microseal 'P+' assembly and seal tightly for thermal cycling when the
pads provide improved sealing of low-volume reactions — cycler lid is closed. They can be reused up to 50 times.
as low as 5 µl in 96-well plates and 1 µl in 384-well plates. The lids are constructed of metal with an attached
Each pad may be used for approximately 25 runs. compressible pad. Two varieties are available: a self-
releasing arched lid and an arched lid with wide tabs
for robotic grippers.
For More Information
Web: bio-rad.com/pcrseals
Ordering Information
Catalog # Description Price, U.S. $
Microseal 'P' and 'P+' Pads
MSP1001 Microseal 'P' Pads, reusable, for Power Bonnet lids, 10 97.00
MSP1002 Microseal 'P+' Pads, reusable, for Moto Alpha unit lids, 10 97.00
MSP1003 Microseal 'P' Replacement Pads, for MSL-2032, reusable, 10 97.00
Auto-Sealing Lids for PCR Plates
MSL2022 Arched Auto-Sealing PCR Plate Lids, reusable, 4 135.00
MSL2032 Arched Auto-Sealing PCR Plate Lids with Wide Tabs, reusable, 4 165.00
Ordering Information
Catalog # Description Price, U.S. $
CHO1401 Chill-out Liquid Wax, red, 100 ml 44.00
CHO1404 Chill-out Liquid Wax, red, 1 L 300.00
CHO1411 Chill-out Liquid Wax, clear, optical grade, 100 ml 53.00
CHO1414 Chill-out Liquid Wax, clear, optical grade, 1 L 300.00
Ordering Information
Catalog # Description Price, U.S. $
SLF0201 Frame-Seal Incubation Chambers, 9 x 9 mm, 25 µl capacity, coverslips included, 100 88.00
SLF0601 Frame-Seal Incubation Chambers, 15 x 15 mm, 65 µl capacity, coverslips included, 100 94.00
SLF1201 Frame-Seal Incubation Chambers, 17 x 28 mm, 125 µl capacity, coverslips included, 100 110.00
SLF3001 Frame-Seal Incubation Chambers, 19 x 60 mm, 300 µl capacity, coverslips included, 100 150.00
Subject Index
C1000 Touch™ Thermal Cycler, 357 Microseal® 96-Well PCR Plates, 394
Capping Tools and Racks, 390 Microseal® 384-Well Skirted PCR Plates, 396
CFX96 Touch™ Deep Well Real-Time PCR Detection System, 364 Multiplate™ 48-Well PCR Plates, 391
CFX96 Touch™ Real-Time PCR Detection System, 363 Multiplate™ 96-Well PCR Plates, 393
CFX384 Touch™ Real-Time PCR Detection System, 365 One-Step RT-ddPCR Advanced Kit for Probes, 353
CFX Automation System II, 366 PCR Plate Seals, 397
CFX Connect™ Real-Time PCR Detection System, 362 PCR Tubes and Strips, 389
CFX Manager™ Software, 368 Precision Melt Analysis™ Software, 369
™
CFX Manager Software, Chinese and Russian Editions, 369 Precision Melt Supermix (HRM), 382
CFX Manager™ Software, Security Edition, 368 PrimePCR™ Assays and Panels, 370
™
CFX Qualification Plate, 367 PrimePCR™ ddPCR Assays, 355
Chill-out™ Liquid Wax, 401 PX1™ PCR Plate Sealer, 386
ddPCR™ Library Quantification Kits, 354 QX200™ ddPCR™ EvaGreen Supermix , 354
ddPCR™ Mutation Screening Kits, 356 QX200™ Droplet Digital™ PCR System, 350
ddPCR™ SMN1 Copy Number Determination Kit, 355 Reaction Modules, 359
ddPCR™ SMN2 Copy Number Determination Kit, 356 S1000™ Thermal Cycler, 358
ddPCR™ Residual DNA Quantification Supermix and Kits, 352 Sealing Pads for Automation, 400
ddPCR™ Supermix for Probes, 353 SingleShot™ Cell Lysis Kits, 381
ddPCR™ Supermix for Probes (No dUTP), 352 SsoAdvanced™ PreAmp Supermix, 380
Frame-Seal™ Incubation Chambers, 401 SsoAdvanced™ Universal Inhibitor-Tolerant SYBR®
Hard-Shell® 96-Well PCR Plates, 392 Green Supermix, 376
Hard-Shell® 384-Well 480 PCR Plates, 396 SsoAdvanced™ Universal Probes Supermix, 374
Hard-Shell® 384-Well Standard PCR Plates, 395 SsoAdvanced™ Universal SYBR® Green Supermix, 373
Heat Sealing Films and Foils, 399 T100™ Thermal Cycler, 360
™
iQ High-Profile 96-Well Semi-Skirted Real-Time PCR Plates, 394
iQ™ Supermixes, 377
iScript™ Reverse Transcription Kits, 383
iTaq™ Universal Probes One-Step Kit, 379
iTaq™ Universal Supermixes, 375
iTaq™ Universal SYBR® Green One-Step Kit, 378
Pipets
A full set of high-precision micropipets is available for all your liquid handling needs.
Professional Micropipets
These adjustable-volume digital pipets deliver
exceptional performance and are guaranteed to function
efficiently and reliably for years. Micropipets are available
in five volume ranges: 0.1–2.0, 0.5–10, 2–20, 20–200,
and 100–1,000 µl.
Micropipet features include:
■■
Adjustable digital dial with volume lock
■■
Improved ergonomic handle and tip ejector Professional Micropipets
■■
ccommodation of standard pipet tips and
A
serological pipets Professional Micropipet Specifications
Adjustable-
■■
3-year warranty*
Volume Volume
■■
Fully autoclavable* Range, µl Channels Increments, µl
404 | Plastic Consumables and General Laboratory Equipment In the U.S., call toll free at 1-800-424-6723
Micropipets, Tips, and Micro Test Tubes
bio-rad.com/pipettips Pipet Tips
Ordering Information
Catalog # Description Price, U.S. $
1660499 Professional Micropipet, adjustable volume, 0.1–2.0 µl 337.50
1660505 Professional Micropipet, adjustable volume, 0.5–10 µl 337.50
1660506 Professional Micropipet, adjustable volume, 2–20 µl 337.50
1660507 Professional Micropipet, adjustable volume, 20–200 µl 337.50
1660508 Professional Micropipet, adjustable volume, 100–1,000 µl 337.50
1660496 Professional Micropipet, 8-channel, adjustable volume, 5–50 µl 698.75
1660495 Professional Micropipet, 8-channel, adjustable volume, 20–200 µl 698.75
1660486 Professional Micropipet Set with Backpack, set includes 4 professional series micropipets: P10, P20, 1,198.75
P200, P1000, carousel pipet rack, 4 racks of pipet tips (TBR-14, -35, -40, Prot/Elec), and Bio-Rad backpack
1660490 Professional Pipet Controller, 120 V, 0.1–100 ml, includes bench stand, charger, wall mount, 0.45 µm filter 373.75
1660491 Professional Pipet Controller, 220 V, 0.1–100 ml, includes bench stand, charger, wall mount, 0.45 µm filter 373.75
1660487 Carousel Pipet Rack, holds 6 single-channel micropipets 158.75
Pipet Tips
Bio-Rad’s pipet tips are made from virgin polypropylene ■■
ll materials used in both clear and colored pipet tips
A
and have been accurately molded for an airtight fit. have been formulated without heavy metals
The tips are guaranteed to have a smooth interior surface, ■■
Xcluda™ pipet tips have been independently tested and
which is essential for precision pipetting. certified to be free of DNase, RNase, and pyrogens
■■
ips and racks are autoclavable at 120°C at 15 lb
T ■■
ipet tip racks are manufactured from polypropylene,
P
of pressure for 15 min; tips presterilized by e-beam with a plastic content code of 5, and are free of labels for
irradiation are also available convenient recycling
In the U.S., call toll free at 1-800-424-6723 Plastic Consumables and General Laboratory Equipment | 405
Micropipets, Tips, and Micro Test Tubes
Pipet Tips bio-rad.com/pipettips
Ordering Information
Catalog # Description Price, U.S. $
2112001 Xcluda Style A, 0.5–10 µl, 960 180.00
2112006 Xcluda Style B, 2–20 µl, 960 180.00
2112011 Xcluda Style C, 10–100 µl, 960 180.00
2112016 Xcluda Style D, 20–200 µl, 960 165.00
2112021 Xcluda Style E, 100–1,000 µl, 960 165.00
2112026 Xcluda Style F, 0.5–10 µl, 1,000 165.00
2112031 Xcluda Style G, 10–100 µl, 1,000 165.00
2112036 Xcluda Style H, 100–1,000 µl, 1,000 165.00
2112041 Xcluda Style J, 10–100 µl, 960 160.00
1660491 Professional Pipet Controller, 220 V, 0.1–100 ml, includes bench stand, charger, wall mount, 0.45 µm filter 373.75
1660492 Professional Pipet Controller, 220 V for Australia, 0.1–100 ml, includes bench stand, charger, wall mount, 373.75
0.45 µm filter
1660487 Carousel Pipet Rack, holds 6 single-channel micropipets 158.75
406 | Plastic Consumables and General Laboratory Equipment In the U.S., call toll free at 1-800-424-6723
Micropipets, Tips, and Micro Test Tubes
bio-rad.com/pipettips Pipet Tips
Ordering Information
Catalog # Description Price, U.S. $
2239911 Seque/Pro Capillary Pipet Tips, in enclosed autoclavable rack, 0.5–10 µl, 200 51.00
2239912 Seque/Pro Capillary Pipet Tips, sterilized in enclosed rack, 0.5–10 µl, 200 55.00
2239915 Prot/Elec Pipet Tips, bulk pack, plastic bag in dust-free box, 1–200 µl, 1,000 59.00
2239917 Prot/Elec Pipet Tips, racked, 12 x 17 format, 204 per rack with a cover on each rack, 1–200 µl, 1,020 91.00
2239916 Prot/Elec Pipet Tips, racked, 8 x 12 format, 96 per rack with a cover on each rack, 1–200 µl, 960 120.00
In the U.S., call toll free at 1-800-424-6723 Plastic Consumables and General Laboratory Equipment | 407
Micropipets, Tips, and Micro Test Tubes
Pipet Tips bio-rad.com/pipettips
MTP
8 x 12 format, enclosed, nonflex racks are suitable for repeated reloading
and autoclaving. Racked tips are also available presterilized.
Ordering Information
Catalog # Description Price, U.S. $
MTP 8 x 12 Format, Enclosed Racks
2239301 MTP-26 Tips, clear, 5–200 µl, 960 62.00
2239303 MTP-35 Tips, yellow, 1–200 µl, 960 63.00
2239304 MTP-37 Tips, clear, 1–200 µl, 960 63.00
2239313 MTP-38 Tips, yellow, graduated, beveled, 1–200 µl, 960 63.00
MTP-S 8 x 12 Format, Enclosed Racks, Presterilized*
2239307 MTP-28-S Tips, clear, 25–200 µl, 960 76.00
2239308 MTP-35-S Tips, yellow, 1–200 µl, 960 76.00
2239309 MTP-37-S Tips, clear, 1–200 µl, 960 76.00
2239318 MTP-38-S Tips, yellow, graduated, beveled, 1–200 µl, 960 76.00
BR Bulk Tips
2239014 BR-14 Tips, clear, 0.1–10 µl, 1,000 38.00
2239028 BR-28 Tips, clear, 25–200 µl, 1,000 38.00
2239031 BR-31 Tips, clear, 0.5–10 µl, 1,000 38.00
2239035 BR-35 Tips, yellow, 1–200 µl, 1,000 38.00
2239037 BR-37 Tips, clear, 1–200 µl, 1,000 38.00
2239038 BR-38 Tips, yellow, graduated, beveled, 1–200 µl, 1,000 38.00
2239039 BR-39 Tips, clear, graduated, beveled, 1–200 µl, 1,000 38.00
2239040 BR-40 Tips, blue, 100–1,000 µl, 500 19.00
2239041 BR-41 Tips, clear, 100–1,000 µl, 500 19.00
RBR Racked Tips, One Cover on Nested Racks
2239135 RBR-35 Tips, yellow, 1–200 µl, 1,000 49.00
TBR Racked Tips, Cover on Each Rack
2239354 TBR-14 Tips, clear, 0.1–10 µl, 1,000 58.00
2239347 TBR-35 Tips, yellow, 1–200 µl, 1,000 58.00
2239350 TBR-40 Tips, blue, 100–1,000 µl, 1,000 61.00
2239351 TBR-41 Tips, clear, 100–1,000 µl, 1,000 60.00
* Presterilized racks and polyethylene plugs are not suitable for autoclaving. Order unsterilized racks to reload tubes and autoclave.
408 | Plastic Consumables and General Laboratory Equipment In the U.S., call toll free at 1-800-424-6723
Micropipets, Tips, and Micro Test Tubes
bio-rad.com/microtesttube Micro Test Tubes
Ordering Information
Catalog # Description Price, U.S. $
2239390 Titertube Micro Test Tubes, unsterilized, 10 racks of 96 tubes 51.00
2239395* Titertube Micro Test Tubes, presterilized, 10 racks of 96 tubes 58.00
2239391 Titertube Micro Test Tubes, unsterilized, bulk, 1,000 39.00
2239393 Titertube Plugs, unsterilized, 960 in 120 strips of 8 19.00
2239392* Titertube Plugs, presterilized, 960 in 120 strips of 8 24.00
2239394 Titertube Rack, empty, holds 96 Titertube micro test tubes, 10 15.00
* Presterilized racks and polyethylene plugs are not suitable for autoclaving. Order unsterilized racks to reload tubes and autoclave.
EZ Micro graduated polypropylene test tubes have a The micro test tubes are:
frosted marking area and a flat top that is pierceable by a ■■
Suitable for general-purpose benchtop centrifuge use;
19 gauge or thicker needle. Standard polypropylene micro sturdy uniform walls easily withstand up to 13,000 x g
test tubes are recommended for solvent extraction and
heating when a very tight cap seal is required. Because of
■■
Autoclavable* to 120°C; freezable to –80°C
their tighter cap fit, standard tubes may be more difficult to ■■
Available in 2 ml, 1.5 ml, and 500 µl sizes
manipulate in applications that require repeated opening
and closing of the tubes. Separate polyethylene caps for the
1.5 ml capless tubes have a knurled top for easy handling.
For More Information
Web: bio-rad.com/ezmicro
Request or download bulletin: 5676
* 1.5 ml Colored Microcentrifuge Tubes are not autoclavable.
In the U.S., call toll free at 1-800-424-6723 Plastic Consumables and General Laboratory Equipment | 409
Micropipets, Tips, and Micro Test Tubes
Reservoirs, EIA Plates, and Additional Plastic Products bio-rad.com/microtesttube
Ordering Information
Catalog # Description Price, U.S. $
2239503 EZ Micro Test Tubes, 500 µl, clear, 1,000 45.00
2239480 EZ Micro Test Tubes, 1.5 ml, clear, 500 29.00
2239430* EZ Micro Test Tubes, 2 ml, clear, 500 31.00
2239501 Micro Test Tubes, standard, 1.5 ml, clear, 500 29.00
2239500 Micro Test Tubes, capless, 1.5 ml, polypropylene, clear, graduated, 500 24.00
2239490 Separate Caps, for capless micro test tubes, white, 1,000 22.00
1660473 Colored Microcentrifuge Tubes, 1.5 ml, 6 colors, 600 40.00
* To fit centrifuges, 2 ml tubes have thinner walls than standard tubes and are intended for short runs at less than 13,000 x g.
Heavy samples, high g forces, long runs, or centrifuges that radiate excessive heat may damage these tubes
Ordering Information
Catalog # Description Price, U.S. $
2240165 0.5 ml Conical Tubes, with installed O-ring screwcaps, sterilized, 500 135.00
2240185 0.5 ml Skirted Tubes, with installed O-ring screwcaps, sterilized, 500 135.00
2240100 1.5 ml Conical Tubes, with separate O-ring screwcaps, unsterilized, 500 92.00
2240110 1.5 ml Conical Tubes, with installed O-ring screwcaps, sterilized, 500 135.00
2240130 2.0 ml Skirted Tubes, with separate O-ring screwcaps, unsterilized, 500 92.00
2240140 2.0 ml Skirted Tubes, with installed O-ring screwcaps, sterilized, 500 135.00
Ordering Information
Catalog # Description Price, U.S. $
2244872 Sterilized Reagent Reservoirs, graduated, polystyrene, 5 per package, box of 200 155.00
410 | Plastic Consumables and General Laboratory Equipment In the U.S., call toll free at 1-800-424-6723
Micropipets, Tips, and Micro Test Tubes
bio-rad.com/liquidhandling Reservoirs, EIA Plates, and Additional Plastic Products
Ordering Information
Catalog # Description Price, U.S. $
2240096 Costar 96-Well Flat Bottom EIA Plates, polystyrene, 5 per package, box of 100 375.00
Tube Rack
Ordering Information
Catalog # Description Price, U.S. $
1660481 Green Racks, hold 80 x 1.5/2.0 ml tubes, set of 5 74.29
1660483 Tube Racks, hold 60 x 15 ml tubes, set of 5 92.50
1660484 Tube Racks, hold 24 x 50 ml tubes, set of 5 92.50
1660482 Storage Boxes, hold 100 x 1.5/2.0 ml tubes, set of 5, multicolored 112.86
In the U.S., call toll free at 1-800-424-6723 Plastic Consumables and General Laboratory Equipment | 411
General Laboratory Equipment
bio-rad.com/labequipment
Ordering Information
Catalog # Description Price, U.S. $
1660476 Cell Culture Tubes, 17 x 100 mm, 14 ml, sterile, 25 22.50
1660475 Conical Centrifuge Tubes, 15 ml, 50 33.75
1660470 Petri Dishes, 60 mm diameter, sterile, 500 161.25
1660471 Inoculation Loops, 10 µl, sterile, 80 16.88
1660474 Disposable Plastic Transfer Pipets, sterile, 1 ml, graduated, 500 135.00
1660480 Disposable Plastic Transfer Pipets, nonsterile, 1 ml, graduated, 500 67.50
1660477 Gel Staining Trays, 4 22.50
1660479 Jellyfish Foam Floating Racks, 8 40.00
412 | Plastic Consumables and General Laboratory Equipment In the U.S., call toll free at 1-800-424-6723
General Laboratory Equipment
bio-rad.com/labequipment
Ordering Information
Catalog # Description Price, U.S. $
1660531 UView Transilluminator 1,120.00
Centrifuges
■■
ini centrifuge — includes microtube and PCR strip tube
M
rotors and 0.4 and 0.5 ml tube adaptors; the maximum
speed is 6,000 rpm (2,000 x g)
■■
odel 16K microcentrifuge — accommodates 1.5
M
or 2.0 ml tubes and has a quick-spin feature; the
maximum speed is 14,000 rpm (16,000 x g). Safe for
coldroom operation
■■
CR tube adaptor for model 16K microcentrifuge —
P
holds two PCR 8-tube strips or 16 individual
0.2 ml tubes
For More Information Model 16K Microcentrifuge Mini Centrifuge
(PCR tube adaptor sold separately) (includes PCR tube adaptor)
Web: bio-rad.com/centrifuges
Ordering Information
Catalog # Description Price, U.S. $
1660603 Mini Centrifuge, 120 V 427.14
1660613 Mini Centrifuge, 220 V 427.14
1660623 Mini Centrifuge, 220 V (UK) 427.14
1660602 Model 16K Microcentrifuge, 120 V 2,784.29
1660612 Model 16K Microcentrifuge, 220 V 2,784.29
1660620 PCR Tube Adaptor, for model 16K microcentrifuge 156.00
In the U.S., call toll free at 1-800-424-6723 Plastic Consumables and General Laboratory Equipment | 413
General Laboratory Equipment
bio-rad.com/labequipment
Ordering Information
Catalog # Description Price, U.S. $
1660710 Mini Rocker, 120 V, includes two blotting boxes 607.14
1660720 Mini Rocker, 230 V (EU/UK), includes two blotting boxes 607.14
1660711 Tube Roller, 120 V, includes 3 tube carousels for 1.5, 15, and 50 ml tubes 727.14
1660721 Tube Roller, 230 V, includes 3 tube carousels for 1.5, 15, and 50 ml tubes 727.14
1660722 Tube Roller, 230 V (UK), includes 3 tube carousels for 1.5, 15, and 50 ml tubes 727.14
1660610 BR-2000 Vortexer, 120 V 412.86
1660611 BR-2000 Vortexer, 230 V 412.86
1660621 BR-2000 Vortexer, 230 V (UK) 412.86
1660622 Flathead Dimpled Adaptor, for BR-2000 vortexer 74.29
1660709 UltraRocker Rocking Platform, 120 V 1,162.86
1660719 UltraRocker Rocking Platform, 230 V 1,162.86
■■
igital dry bath — digitally controlled dry bath that is ideal
D
for a multitude of laboratory procedures where incubation of
samples is needed. It is accurate, built for safe continuous
operation, economical, and versatile. Comes with one
aluminum alloy 1.5 ml heating block (24 x 1.5 ml capacity).
Additional 0.5, 2.0, and 15 ml heating blocks with 24 x 0.5,
0.5 ml 2.0 ml
24 x 2.0, and 12 x 15 ml capacity, respectively, can be Digital Dry Bath Dry Bath Block Dry Bath Block
purchased separately; 15 ml dry bath block not shown
■■
ini incubation oven — can accommodate up to eighty
M
6.5 cm plates and operates at temperatures up to 60°C.
Contains a rear port to allow insertion of our tube roller or
mini rocker for temperature-controlled mixing
■■
ater bath — temperature controlled, dependable,
W
affordable water bath that includes stainless steel tank and lid,
electrostatically applied finish that resists rust, corrosion, and
scratches, over-temperature protection, and thermometer
Mini Incubation Oven Water Bath
■■
yNA Chill cooler — keeps samples (up to 12 in
D
1.5–2.0 ml microtubes) chilled. Simply cool the DyNA
Chill cooler to the desired temperature overnight, then
chill samples without the mess of an ice bucket
For More Information
Web: bio-rad.com/tempcontrol
DyNA Chill Cooler
Ordering Information
Catalog # Description Price, U.S. $
1660562 Digital Dry Bath, 120 V, includes 1.5 ml heating block 655.71
1660563 Digital Dry Bath, 230 V (EU/UK), includes 1.5 ml heating block 655.71
1660565 Digital Dry Bath Heating Block, 0.5 ml, for 24 x 0.5 ml tubes 127.50
1660566 Digital Dry Bath Heating Block, 2.0 ml, for 24 x 2.0 ml tubes 127.50
1660567 Digital Dry Bath Heating Block, 15 ml, for 12 x 15 ml tubes 127.50
1660564 DyNA Chill Cooler, maintains sample temperature between –15°C and room temperature 112.86
for up to 8 hr without ice
1660501 Mini Incubation Oven, 120 V 570.00
1660521 Mini Incubation Oven, 230 V 570.00
1660504 Temperature-Controlled Water Bath, 120 V 791.43
1660524 Temperature-Controlled Water Bath, 230 V 791.43
1660712 Mini Incubation Oven and Mini Rocker, 120 V Inquire
1660713 Mini Incubation Oven and Tube Roller, 120 V Inquire
414 | Plastic Consumables and General Laboratory Equipment In the U.S., call toll free at 1-800-424-6723
Plastic Consumables and General Laboratory Equipment
Subject Index
Subject Index
96-Well EIA Plates, 411
Additional Plastic Consumables, 412
Centrifuges, 413
EZ Micro™ and Standard Micro Test Tubes, 409
Micropipets and Accessories, 404
Mixing Devices, 413
Pipet Tips for Gel Loading, 407
Reagent Reservoirs, 410
Screwcap Micro Test Tubes, 410
Standard Pipet Tips, 407
Temperature Control Equipment, 414
Titertube® Micro Test Tubes, 409
Tube Racks and Storage Boxes, 411
UView™ Transilluminator, 412
Xcluda™ Aerosol Barrier Pipet Tips, 406
In the U.S., call toll free at 1-800-424-6723 Plastic Consumables and General Laboratory Equipment | 415
416 | Plastic Consumables and General Laboratory Equipment In the U.S., call toll free at 1-800-424-6723
Biotechnology Explorer ™
Educational Products
Captivating Science Education 418
Project-Based Learning Modular Laboratory Explorer Series 420
Rapid Blotting and V3 Western Workflow™ 421
Real-Time PCR 422
Classroom Kits 423
Captivating Science Education
explorer.bio-rad.com
■■
Online teaching resources and experimental design
For More Information
Web: bio-rad.com/algae1
418 | Biotechnology Explorer Educational Products In the U.S., call toll free at 1-800-424-6723
Captivating Science Education
explorer.bio-rad.com
Ordering Information
Catalog # Description Price, U.S. $
Inquire hotosynthesis and Cellular Respiration Kit, includes algae beads, colorimetric indicator solution, ThINQ!
P Inquire
teacher and student manuals, and other accessories; provides materials for 32 students or 8 workstations
EDU price discounts are for qualified educational institutions and educators only. Items are available at list price
for noneducators (must be ordered without an EDU suffix).
What happens if one fish gets substituted for another? contributing to the global species barcoding initiative
Most of the time, the consumer won’t even notice. while also learning advanced genetic analysis skills.
But what happens if substituting a less expensive fish ■■
Real-world application
for a more expensive one becomes a common practice? ■■
Inquiry-based hands-on laboratory
Or if a poisonous fish like pufferfish makes it into our food ■■
Aligns with AP Biology Big Ideas 1, 2, 3, and 4
supply, or we deplete our oceans of critically endangered
species? Bio-Rad’s Fish DNA Barcoding kit helps For More Information
students answer these and similar real-world questions, Web: bio-rad.com/fishbarcoding
Perform sequencing
Select fish Extract fish DNA Amplify COI gene Perform DNA
Fish DNA sample electrophoresis
and bioinformatics
analysis
Barcoding
Flowchart
Ordering Information
Catalog # Description Price, U.S. $
1665100EDU ish DNA Barcoding Kit, includes reagents for DNA extraction and PCR for up to 16 fish samples.
F 347.50
Purchase sequencing module (US only) separately
1665115EDU DNA Barcoding Sequencing Module, prepaid sequencing service for up to 9 samples. 123.75
Includes shipping of samples. Valid only for use with 1665100EDU. U.S. only
EDU price discounts are for qualified educational institutions and educators only. Items are available at list price
for noneducators (must be ordered without an EDU suffix).
This laboratory textbook blends textbook theory with The teacher supplement provides a thorough background
hands-on laboratory activities and real-world applications on preparation, setup, results analysis, and assessment.
for your biotechnology course, and it incorporates It also provides guidance on how to implement and build
Biotechnology Explorer™ kits for easy implementation your biotechnology course. Chapters include:
supported by live technical support. This textbook
encourages the next generation of biotechnologists by:
■■
The Biotechnology Industry
■■
Laboratory Skills
■■
Developing key skills with multiple activities ■■
Microbiology and Cell Culture
■■
ncouraging students to consider the broader
E ■■
DNA Structure and Analysis
implications of biotechnology with bioethics case studies ■■
Bacterial Transformation and Plasmid Purification
■■
Broadening occupational ■■
Polymerase Chain Reaction
awareness with profiles ■■
Protein Structure and Analysis
of careers in biotech ■■
Immunological Applications
■■
Research Projects
■■
etting students answer a
L
research question using For More Information
independent research Web: explorer.bio-rad.com/textbook
In the U.S., call toll free at 1-800-424-6723 Biotechnology Explorer Educational Products | 419
Captivating Science Education
Modular Laboratory Explorer Series explorer.bio-rad.com
Ordering Information
Catalog # Description Price, U.S. $
1661027EDU iotechnology: A Laboratory Skills Course, teacher edition, includes one student edition
B 150.00
and one teacher supplement
1661025EDU Biotechnology: A Laboratory Skills Course, student edition 117.50
1661026EDU Biotechnology: A Laboratory Skills Course, teacher supplement 43.75
1661051EDU Laboratory Notebook 18.75
1661052EDU Supplementary Materials DVD Set 15.00
EDU price discounts are for qualified educational institutions and educators only. Items are available at list price
for noneducators (must be ordered without an EDU suffix).
420 | Biotechnology Explorer Educational Products In the U.S., call toll free at 1-800-424-6723
Captivating Science Education
explorer.bio-rad.com Rapid Blotting and V3 Western Workflow
Ordering Information
Catalog # Description Price, U.S. $
1665000EDU* Complete Cloning and Sequencing Explorer Series, includes all 8 modules and curriculum resource CD 1,906.25
(sequencing service not included)
1665005EDU* Nucleic Acid Extraction Module 215.00
1665010EDU* GAPDH PCR Module 498.75
1660451EDU Electrophoresis Module 133.33
7326300EDU PCR Kleen Spin Purification Module 78.75
1665015EDU* Ligation and Transformation Module 406.25
1665020EDU Microbial Culturing Module 121.25
7326400EDU Aurum Plasmid Mini Purification Module 217.14
1665025EDU* Sequencing and Bioinformatics Module, includes sequencing primers, control plasmid, 327.50
and bioinformatics subscription; sequencing service not included
1665001EDU Curriculum Resource CD 37.50
1665040EDU* Protein Expression and Purification Series, centrifugation purification process 723.75
1665045EDU* Protein Expression and Purification Series, hand-packed purification process 916.25
1665050EDU* Protein Expression and Purification Series, prepacked purification process 916.25
1665070EDU Protein Expression and Purification Series Assessment Module, formative and summative 63.75
assessment tool
1665055EDU* Growth and Expression Module 317.50
1665060EDU* SDS-PAGE Electrophoresis Module 223.75
1665041EDU Centrifugation Purification Module 70.00
1665046EDU Hand-Packed Purification Module 262.50
1665051EDU Prepacked Purification Module 262.50
1665065EDU* DHFR Enzymatic Assay Module 113.75
* Ships with both temperature-sensitive and room temperature components. Immediately store temperature-sensitive items
at 4°C or –20°C as indicated.
EDU price discounts are for qualified educational institutions and educators only. Items are available at list price for
noneducators (must be ordered without an EDU suffix).
Western Blotting in Less than 2 Hours! Hands-On Time Expenditure (in minutes)
The new rapid blotting or V3 Western Workflow (stain-free
rapid blotting) allows you to complete the entire western
blot workflow in less than 2–5 hours in the classroom,
depending on which time-saving steps are incorporated. Rapid V3 Western
TGX Stain-Free™ gels combined with the super-fast blotting Workflow
Trans-Blot® Turbo™ transfer system provide maximum Tank (staining (stain-free
time savings, allowing you to complete the workflow in blotting required) rapid blotting)
less than a single 3-hour lab block. Teach students about Protein extraction 33 33 33
and electrophoresis
the exciting new chemistry that allows visualization of
samples separated on PAGE gels without staining. Protein visualization 180 180 <3
Protein transfer
Benefits: Equilibration 15 0 0
Setup 30 5 5
■■
Time savings Transfer 30–150 15 15
■■
Stain-free
Immunoblotting 45 45 45
For More Information Color detection All 10 min–overnight
Web: explorer.bio-rad.com/rapidblotting
Total hands-on time 343–463 288 111
(5 hrs 43 min (4 hrs 48 min) (1 hr 51 min)
to 7 hrs 43 min)
Total Time Savings of 55 min to 7 1/2 hrs
In the U.S., call toll free at 1-800-424-6723 Biotechnology Explorer Educational Products | 421
Captivating Science Education
Real-Time PCR www.explorer.bio-rad.com
Ordering Information
Catalog # Description Price, U.S. $
1662875EDU apid Blotting and V3 Western Workflow Starter Kit, comprehensive protein classroom study kit,
R 717.50
includes protein profiler module (#1662700EDU), western blot module (#1662800EDU), Trans-Blot Turbo
mini nitrocellulose transfer pack, TGX Stain-Free gels, application note, for 32 students
EDU price discounts are for qualified educational institutions and educators only. Items are available at list price for
noneducators (must be ordered without an EDU suffix).
Real-Time PCR
Ordering Information
Catalog # Description Price, U.S. $
1662660EDU Crime Scene Investigator PCR Basics Real-Time PCR Starter Kit 690.00
1662560EDU GMO Investigator Real-Time PCR Starter Kit 700.00
EDU price discounts are for qualified educational institutions and educators only. Items are available at list price for
noneducators (must be ordered without an EDU suffix).
422 | Biotechnology Explorer Educational Products In the U.S., call toll free at 1-800-424-6723
Captivating Science Education
explorer.bio-rad.com Classroom Kits
Classroom Kits
Ordering Information
Catalog # Description Price, U.S. $
1665075EDU IDEA Kit — Inquiry Dye Electrophoresis Activity, provides materials for 32 students or 8 workstations 68.75
1665080EDU STEM Electrophoresis Teacher Demonstration Kit, provides materials for 8 students or 2 workstations 123.75
1665090EDU STEM Electrophoresis Classroom Kit, provides materials for 32 students or 8 workstations 342.50
1660003EDU pGLO Bacterial Transformation Kit, provides materials for 32 students or 8 workstations 118.75
1660013EDU* pGLO Kit SDS-PAGE Extension, provides materials for 32 students or 8 workstations 128.00
1660005EDU* Green Fluorescent Protein (GFP) Chromatography Kit, provides materials for 32 students or 8 workstations 130.00
1660006EDU* Secrets of the Rainforest Kit, provides materials for 32 students or 8 workstations 215.00
1665030EDU Microbes and Health Kit, provides materials for 32 students or 8 workstations 123.75
1660500EDU Long-Wave UV Lamp, requires 4 AA batteries 46.25
1660530EDU Long-Wave UV Penlight 15.00
1660008EDU Size Exclusion Chromatography Kit, provides materials for 32 students or 8 workstations 106.25
1662900EDU* Got Protein? Kit, provides materials for 320 students or 80 workstations 226.25
1662400EDU* ELISA Immuno Explorer Kit, provides materials for 48 students or 12 workstations 172.50
1662700EDU* Comparative Proteomics Kit I: Protein Profiler Module, provides materials for 32 students or 8 workstations 222.50
1662800EDU* Comparative Proteomics Kit II: Western Blot Module, provides materials for 32 students or 8 workstations 248.75
1665035EDU* Biofuel Enzyme Kit, provides materials for 32 students or 8 workstations 168.75
1660007EDU* Forensic DNA Fingerprinting Kit, provides materials for 32 students or 8 workstations 167.50
1660001EDU* Analysis of Precut Lambda DNA Kit, provides materials for 32 students or 8 workstations 120.00
1660002EDU* Restriction Digestion and Analysis of Lambda DNA Kit, provides materials for 32 students or 8 workstations 135.00
1662300EDU Genes in a Bottle Kit, includes 1 DNA extraction module (#1662000EDU) and DNA necklace module 167.50
(#1662250EDU); provides materials for 36 students or 9 workstations
1662600EDU* Crime Scene Investigator PCR Basics Kit, provides materials for 32 students or 8 workstations 220.00
1662100EDU* PV92 PCR Informatics Kit, provides materials for 32 students or 8 workstations 262.50
1662500EDU* GMO Investigator Kit, provides materials for 32 students or 8 workstations 233.75
* Ships with both temperature-sensitive and room temperature components. Immediately store temperature-sensitive items
at 4°C or –20°C as indicated.
EDU price discounts are for qualified educational institutions and educators only. Items are available at list price for
noneducators (must be ordered without an EDU suffix).
In the U.S., call toll free at 1-800-424-6723 Biotechnology Explorer Educational Products | 423
Biotechnology Explorer Educational Products
Subject Index
Subject Index
Biotechnology: A Laboratory Skills Course, 419
Biotechnology Explorer™ Kits, 423
Fish DNA Barcoding Kit, 419
Photosynthesis and Cellular Respiration Kit, 418
Real-Time PCR Kits, 422
V3 Western Workflow™ (stain-free rapid blotting), 421
424 | Biotechnology Explorer Educational Products In the U.S., call toll free at 1-800-424-6723
Appendices and Index
Trademarks and Legal Notices 426
References 428
Trademarks and Legal Notices
bio-rad.com
426 | Appendices and Index In the U.S., call toll free at 1-800-424-6723
Trademarks and Legal Notices
bio-rad.com
Legal Notices
The Bio-Plex suspension array system includes Purchase of Criterion XT Bis-Tris gels, XT MOPS running buffer, XT MES
fluorescently labeled microspheres and running buffer, XT MOPS buffer kit, and XT MES buffer kit is accompanied
instrumentation licensed to Bio-Rad Laboratories, by a limited license under U.S. Patent Numbers 6,143,154; 6,096,182;
Inc. by the Luminex Corporation. CST antibodies 6,059,948; 5,578,180; 5,922,185; 6,162,338; and 6,783,651, and
exclusively developed and validated for Bio-Plex phosphoprotein and corresponding foreign patents.
total target assays. Strep-tag technology for western blot detection is covered by U.S.
Profinity eXact vectors, tags, and resins are exclusively licensed under Patent Number 5,506,121 and by UK Patent Number 2,272,698.
patent rights of Potomac Affinity Proteins. This product is intended for StrepTactin is covered by German patent application P 19641876.3.
research purposes only. For commercial applications or manufacturing Bio-Rad Laboratories, Inc. is licensed by Institut für Bioanalytik GmbH to
using these products, commercial licenses can be obtained by contacting sell these products for research use only.
the Life Science Group Chromatography Marketing Manager, Bio-Rad
Laboratories, Inc., 6000 Alfred Nobel Drive, Hercules, CA 94547, Precision Plus Protein standards are sold under license from Life
Telephone 1-800-4BIORAD (1-800-424-6723). The composition and/ Technologies Corporation, Carlsbad, CA, for use only by the buyer
or use of the T7 expression system is claimed in one or more patents of the product. The buyer is not authorized to sell or resell this product
licensed to Bio-Rad by Brookhaven Science Associates, LLC. A separate or its components.
license is required for any commercial use, including use of these Bio-Rad Laboratories, Inc. is licensed by Invitrogen Corporation to sell
materials for research or production purposes by any commercial entity. SYPRO products for research use only under U.S. Patent Number
Expression and purification of GST fusion proteins may require a 5,616,502.
license under U.S. Patent Number 5,654,176 (assignee: Chemicon Biolistic technology is covered by patents owned by Pfizer Inc.
International). The technology is exclusively licensed to Bio-Rad Laboratories, Inc.
CHEF (U.S. Patent Number 5,549,796, issued to Stanford University) is Bio-Rad’s thermal cyclers are covered by one or more of the following
exclusively licensed to Bio-Rad Laboratories, Inc. U.S. patents or their foreign counterparts owned by Eppendorf AG: U.S.
Patent Numbers 6,767,512 and 7,074,367.
In the U.S., call toll free at 1-800-424-6723 Appendices and Index | 427
References
bio-rad.com
References
Sample Preparation Laemmli UK (1970). Cleavage of structural proteins during the assembly
Chomczynski P and Sacchi N (1987). Single-step method of RNA of the head of bacteriophage T4. Nature 227, 680–685.
isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Lerman LS and Silverstein K (1987). Computational simulation of DNA
Anal Biochem 162, 156–159. melting and its application to denaturing gradient gel electrophoresis.
Vuillard et al. (1995). Enhancing protein solubilization with nondetergent Methods Enzymol 155, 482–501.
sulfobetaines. Anal Biochem 230, 290–294. Li JK et al. (1987). Rapid alkaline blot-transfer of viral dsRNAs.
Anal Biochem 163, 210–218.
Sample Quantitation Merril CR et al. (1981). A rapid silver stain for polypeptides in
Bradford MM (1976). A rapid and sensitive method for the quantitation polyacrylamide gels. Anal Biochem 110, 201–207.
of microgram quantities of protein utilizing the principle of protein-dye Ornstein L and Davis BJ (1959). Disc Electrophoresis. Distillation
binding. Anal Biochem 72, 248–254. Products Industries (Division of Eastman Kodak Co.).
Lowry OH et al. (1951). Protein measurement with the Folin phenol Rohringer R and Holden DW (1985). Protein blotting: detection of
reagent. J Biol Chem 193, 265–275. proteins with colloidal gold, and of glycoproteins and lectins with
biotin-conjugated and enzyme probes. Anal Biochem 144, 118–127.
Electrophoresis and Blotting
Amplification/PCR
Gatti RA et al. (1984). Multiple uses of Southern blots. Biotechniques 2,
148–155. Bustin SA et al (2009). The MIQE guidelines: minimum information for
the publication of quantitative real-time PCR experiments. Clin Chem 55,
Gottlieb M and Chavko M (1987). Silver staining of native and denatured 611–622.
eucaryotic DNA in agarose gels. Anal Biochem 165, 33–37.
428 | Appendices and Index In the U.S., call toll free at 1-800-424-6723
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Email: nordic_helpdesk@bio-rad.com Bio-Rad Laboratories KK Warszawa (Warsaw) Al Barsha (South)
Austria Shinagawa-ku Phone: +48 22 331 99 99 P.O. Box 478831, Dubai
Bio-Rad Laboratories Ges.m.b.H Dominican Republic Phone: 81-3-6361-7000 Email: info_poland@bio-rad.com Phone: +971 4 8187300
Wien (Vienna) Bionuclear Fax: 81-3-5463-8480 Email: lsg_emerging_markets@
Phone: +43 1 877 89 01 177 Santo Domingo Portugal bio-rad.com
Osaka location
Fax: +43 1 876 56 29 Phone: 809 567 8172 Bio-Rad Laboratories Ltda.
Yodogawa-ku
Email: techsupportlsg.ce@bio-rad.com Fax: 809 565 2640 Amadora The following countries are
Phone: 81-6-6308-6568
Email: eperezmella@ Phone: +351 21 4727700 handled through the United Arab
Fax: 81-6-6308-3064
Belgium bionuclear.com.do Fax: +351 21 4727777 Emirates office:
Bio-Rad Laboratories N.V. Email: mlambertus@ Fukuoka location Email: sales_admin_portugal@
3, Winninglaan bionuclear.com.do Hakata-ku bio-rad.com Afghanistan, Albania, Algeria,
9140 Temse Phone: 81-92-475-4856 Angola, Armenia, Azerbaijan,
Phone: +32 (0) 3 710 53 00 Ecuador Fax: 81-92-475-4858 Russian Federation Bahrain, Belarus, Benin,
Fax: +32 (0) 3 710 53 01 AM TEC-LAB Bio-Rad Laboratorii OOO Bosnia and Herzegovina,
Email: benelux_lifescience@ Quito Korea Moscow Botswana, Bulgaria, Burkina Faso,
bio-rad.com Phone: 593 2 382 6753 Bio-Rad Korea Ltd. Phone: (+7) 495 721 14 04 Burundi, Cameroon, Cape Verde,
Fax: 593 2 382 6848 Seoul Email: info_russia@bio-rad.com Central African Republic, Chad,
Bolivia Email: ventas@amtec-lab.com Phone: (82-2) 3473-4460 Croatia, Cyprus, Democratic
Compañia Tecnica y Comercial SRL Fax: (82-2) 3472-7003 Singapore Republic of Congo, Djibouti,
La Paz Finland Email: sales.korea@bio-rad.com Bio-Rad Laboratories Egypt, Equatorial Guinea, Eritrea,
Phone: +59 1 2243 1012 Bio-Rad Laboratories support.korea@bio-rad.com (Singapore) Pte. Ltd. Estonia, Ethiopia, Gabon, Gambia,
Fax: +59 1 2243 4335 Valimotie 1A Phone: (65) 6415 3188 Georgia, Ghana, Greece, Guinea,
BMS (Bio-Medical Science) Co., Ltd. Guinea-Bissau, Iraq, Ireland,
Email: comtec@comtec.com.bo 00380 Helsinki Fax: (65) 6415 3189
Seoul Ivory Coast, Jordan, Kazakhstan,
Phone: +358 (0)9 804 2200 Email: sales.singapore@bio-rad.com
Phone: (82-2) 3471-6500 Kenya, Kosovo, Kuwait, Kyrgyzstan,
Brazil Fax: +358 (0)9 7597 5010 The following country is handled
Fax: (82-2) 3472-1211 Latvia, Lebanon, Lesotho, Liberia,
Main office Email: nordic_helpdesk@bio-rad.com through the Singapore office:
Bio-Rad Laboratórios Brasil Ltda. Libya, Lithuania, Macedonia,
Malaysia Brunei
São Paulo France Madagascar, Malawi, Mali, Malta,
Chemoscience (Malaysia) Sdn Bhd Mauritania, Moldova, Mongolia,
Phone: +55 11 3065-7550 Bio-Rad Laboratories
Selangor South Africa
Fax: 55 (11) 3065-7558 Marnes-la-Coquette Morocco, Mozambique, Namibia,
Phone: +603 7872 6000 Bio-Rad Laboratories (PTY) Ltd.
Email: lsg_brazil@bio-rad.com Phone: 01 47 95 69 65 Niger, Nigeria, Oman, Pakistan,
Fax: +603 7876 3188 Parklands, Johannesburg
Fax: 01 47 95 61 21 Palestine, Qatar, Republic of
Email: enquiry@ Phone: 27 (0) 861 246 723
Canada Montenegro, Republic of Congo,
The following countries are handled chemoscience.com.my Email: info_south-africa@bio-rad.com
Bio-Rad Laboratories (Canada) Ltd. Romania, Rwanda, São Tomé
through the French office: and Principe, Saudi Arabia,
Mississauga, Ontario Spain
French Guyana, Guadeloupe, Mexico Senegal, Serbia, Sierra Leone,
Toll free: 1-800-268-0213 Bio-Rad Laboratories, S.A.
Martinique, Reunion Island Bio-Rad, S.A. Slovak Republic, Slovenia, Somalia,
Phone: 1-905-364-3435 Madrid location
Mexico, D.F. (Mexico City) South Sudan, Sudan, Swaziland,
Fax: 1-888-913-9779 Phone: +34 91 5905200
Germany Phone: (52) 555 488 7670 Syria, Tajikistan, Tanzania, Togo,
Email: sales_canada@bio-rad.com Fax: +34 91 5905217
Bio-Rad Laboratories GmbH Fax: 52 (55) 1107 7246 Tunisia, Turkey, Turkmenistan,
München (Munich) Email: lsg_mexico@bio-rad.com Email: customerservice_spain@
Caribbean Islands bio-rad.com Uganda, Ukraine, Uzbekistan,
Phone: +49 89 31 884 177 Yemen, Zaire, Zambia, Zimbabwe
Scientific Supplies & Technology
Fax: +49 89 31 884 123 The Netherlands Bio-Rad Laboratories, S.A.
(SST) Intl. Inc.
Email: techsupportlsg.ce@ Bio-Rad Laboratories B.V. Barcelona location United Kingdom
7245 NW 43rd St.
bio-rad.com 3, Winninglaan Phone: +34 93 479 15 10 Bio-Rad Laboratories Ltd.
Miami, FL 33166 USA
9140 Temse (Belgium) Fax: +34 93 478 15 00 Hemel Hempstead
Phone: 1-305-593-2137
Hong Kong Phone: +31 (0)318 540 666 Email: customerservice_spain@ Hertfordshire
Fax: 1-305-477-4256
Bio-Rad Pacific Ltd. Fax: +31 (0)318 542 216 bio-rad.com Phone: +44 (0) 20 8328 2000
Email: annieh@sst-intl.com
Quarry Bay Email: benelux_lifescience@ Free phone: 0800 181134
Phone: 852-2789-3300 bio-rad.com Sweden Fax: +44 (0) 20 8328 2550
Chile
Fax: 852-2789-1257 Bio-Rad Laboratories AB
Galenica S.A. Ireland has a local distributor,
Email: sales.hongkong@bio-rad.com New Zealand Solna
Santiago but please direct initial enquiries
Bio-Rad Laboratories Pty. Ltd. Phone: 08 555 12700
Phone: 56 2 2799 2900 to the UK office.
Hungary Auckland Fax: 08 555 12780
Fax: 56 2 799 2950
Bio-Rad Hungary Ltd. Phone: 0508 805 500 Email: nordic_helpdesk@bio-rad.com
Email: info@galenica.cl Uruguay
Budapest Fax: (+64 9) 415 2284
Phone: +36 1 459 6100 Email: sales.nz@bio-rad.com Switzerland Biodiagnostico CabinSur SA
China, People’s Republic of Montevideo
Email: info_hungary@bio-rad.com Bio-Rad Laboratories AG
Bio-Rad Laboratories (Shanghai) Ltd. The following countries are handled Phone/Fax: (598) 2487 1537
China Head Office, Shanghai location through the New Zealand office: Cressier
India Phone: +41 (0)26 674 55 05 Email: gpastoriza@
Toll free tech support: The Pacific Islands, Papua New Guinea biodiagnostico.com.uy
Bio-Rad Laboratories (India) Pvt. Ltd. Fax: +41 (0)26 674 52 19
800 820 5567, 400 820 3630 Email: Export_Pacific@bio-rad.com
Gurgaon Product Support: +41 (0)26 674 53 60
Phone: 86-21-6169 8500 Venezuela
Phone: +91-124-4029300 Email: swiss@bio-rad.com
Fax: 86-21-6169 8599 Norway Loginca C.A.
Fax: 0091-124-2398115
Email: sales.china@bio-rad.com Bio-Rad Laboratories Colinas de Bello Monte, Caracas
Toll free LSG tech support: Taiwan
Nydalsveien 28 Telefax: 58 212 751-9376/5732/9189
Beijing location 1-800-1801224 Bio-Rad Laboratories Taiwan Ltd.
0484 Oslo Email: leonsilvaloginca@gmail.com
Phone: 86-10-5939 0088 Email: sales.india@bio-rad.com Taipei
Phone: 23 38 41 30 tomas.hermoso@gmail.com
Fax: 86-10-5939 0160 support.india@bio-rad.com Phone: (886-2) 2578-7189
Fax: 23 38 41 39
Email: sales.china@bio-rad.com Fax: (886-2) 2578-6890
The following countries are handled Email: nordic_helpdesk@bio-rad.com
Guangzhou location through the India office: Bangladesh, Email: sales.taiwan@bio-rad.com
Phone: 86-20-8732 2339 Bhutan, Nepal, Maldives, Mauritius,
Fax: 86-20-8732 2332 Seychelles, Sri Lanka
Email: sales.china@bio-rad.com
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Bio-Rad
Laboratories, Inc.
Life Science Web site bio-rad.com USA 1 800 424 6723 Australia 61 2 9914 2800 Austria 43 1 877 89 01 177 Belgium 32 (0)3 710 53 00 Brazil 55 11 3065 7550
Canada 1 905 364 3435 China 86 21 6169 8500 Czech Republic 420 241 430 532 Denmark 45 44 52 10 00 Finland 358 09 804 22 00
Group France 33 01 47 95 69 65 Germany 49 89 31 884 0 Hong Kong 852 2789 3300 Hungary 36 1 459 6100 India 91 124 4029300
Israel 972 03 963 6050 Italy 39 02 216091 Japan 81 3 6361 7000 Korea 82 2 3473 4460 Mexico 52 555 488 7670 The Netherlands 31 (0)318 540 666
New Zealand 64 9 415 2280 Norway 47 23 38 41 30 Poland 48 22 331 99 99 Portugal 351 21 472 7700 Russia 7 495 721 14 04
Singapore 65 6415 3188 South Africa 27 (0) 861 246 723 Spain 34 91 590 5200 Sweden 46 08 555 12700 Switzerland 41 026 674 55 05
Taiwan 886 2 2578 7189 Thailand 66 662 651 8311 United Arab Emirates 971 4 8187300 United Kingdom 44 020 8328 2000