SpectroScan 80D IM

INSTRUCTION MANUAL
SpectroScan 80D/80DV
UV-VIS SPECTROPHOTOMETER
SpectroScan 80D/80DV Instruction manual
1- CONTENT
Chapter 1 Installation………………………………………………………………………………………..1
1.1 Inspection of Parts………………………………………………………………………………1
1.2 General Description of the Spectrophotometer………………………………………………………1
1.2.1 Front side of the instrument……………………………………………………………………1
1.2.2 Rear side of the instrument…………………………………………………………………….…2
1.2.3 Display……………………………………………………………………………………………2
1.2.4 Keyboard………………………………………………………………………………………….3
1.3 Site Requirement…………………………………………………………………………………….…4
1.4 Installation………………………………………………………………………………………...…4
1.5 Turning on the instrument…………………………………………………………………………...…4
1.6 Performance Check at Installation……………………………………………………………………..5
1.6.1 Wavelength accuracy and reproducibility…...……………………………………………………5
1.6.2 Baseline flatness…………………………..……………………………………………………5
Chapter 2 Instrument Specifications……………………………………………………………………………6
Chapter 3 Photometric Measurement……………………………………………………………………………7

3.1 Information Display and Function Keys………………………………………………………………7
3.2 Parameter Setting………………………………………………………………………………7
3.2.1 Setting photometric mode………………………………………………………………………8
3.2.2 Setting measuring wavelength………………………………………………………………8
3.2.3 Inputting K-Factor……………………………………………………………………………8
3.2.4 Quitting parameter setting………………………………………………………………………8
3.3 Setting working wavelength………………………………………………………………………9
3.4 Auto-zeroing…………………………………………………………………………………………9
3.5 Sample Control………………………………………………………………………………………9
3.5.1 Selecting sample holder type………………………………………………………………….…10
3.5.2 Setting sample cell number in use……..…………………………………………..…10
3.5.3 Cell one blank correction……………………………………………………………….…11
3.5.4 Sample cell shifting (Move Cell) ………………………………………………………………11
3.5.5 Resetting to cell No.1 (Move Home) ……………………………………………………………11
3.5.6 Single-cell measurement …..………………………………………………………………11
3.5.7 Quitting sample control…………………………………………………………………………11
3.6 Measuring Operation…………………………………………………………………………………..11
3.6.1 Setting measuring wavelength……………………………………………………………………12
3.6.2 Auto zeroing……………………………………………………………………………………12
3.6.3 Starting measurement…………………………………………………………………………….13
3.6.4 Looking up the measured results…………………………………………………………………13
3.6.5 Clearing off the measured data……………………………………………………...……………13
3.6.6 Printing out…………………………………………………………………………..…………13
3.6.7 Quitting measuring operation…………………………………………………………………….14
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Chapter 4 Spectrum Scanning………………………………………………………………………………15

4.1 Information Display and Function Keys……………………………………………………………….15
4.2 Parameter Setting………………………………………………………………………………………15
4.2.1 Setting photometric mode………………………………………………………………………16
4.2.2 Setting scanning speed……………………………………………………………………………16
4.2.3 Setting sampling interval………………………………………………………………….……..17
4.2.4 Inputting wavelength rang…………………………………………………………………..17
4.2.5 Inputting ordinate scale range………………………………………………………………17
4.2.6 Dark current correction………………………………………………………………………17
4.2.7 Quitting parameter settings………………………………………………………………………17
4.3 Setting current wavelength………………………………………………………………………17
4.4 Baseline Correction…………………………………………………………………………………….18
4.5 Measuring Operation………………………………………………………………………………18
4.6 P e a k - p i c k i n g … … … … … … … … … … … … … … … … … … … … … … … … … … … … … … 1 9
4.7 Printing Out Measured Results……………………………………………………………………20
4.8 Quitting Spectrum Scanning Measurement……………………………………………………………20
Chapter 5 Quantitative Measurement ………………….………………………………………………………21

5.1 Information Display and Function Keys………………………………………………………………21
5.2 Parameter Setting………………………………………………………………………………21
5.2.1 Setting measurement method……………………………………………………………………22
5.2.2 Setting measuring wavelength…………………………………………………………………22
5.2.3 Setting concentration unit………………………………………………………………………22
5.2.4 Setting calibration curve parameters for K-Factor method………………………………………23
5.2.4.1 Setting the slope of calibration curve……………………………………………………24
5.2.4.2 Setting the intercept of calibration curve…………………………………………………24
5.2.4.3 Quitting calibration curve parameter setting………………………………………………25
5.2.5 Setting calibration curve parameters for Calibration Curve method……………………………25
5.2.5.1 Setting the number of standards…………………………………………………………25
5.2.5.2 Entering standard sample concentrations and absorbance………………………………26
5.2.5.3 Starting standard sample measurement…………………………………………………27
5.2.5.4 Building the calibration curve……………………………………………………………27
5.2.5.5 Printing out parameters of the calibration curve…………………………………………28
5.2.5.6 Quitting calibration curve parameter setting………………………………………………28
5.2.6 Quitting parameter setting of quantitative measurement…………………………………………28
5.3 Auto Zeroing……………………………………………………………………………………...…28
5.4 Setting current wavelength………………………………………………………………………28
5.5 Measuring Operation………………………………………………………………………………29
5.6 Looking Up the Measured Results……………………………………………………………………30
5.7 Printing Out………………………………………………………………………………………..30
5.8 Quitting Quantitative Measurement…………………………………………………………………30
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Chapter 6 DNA/Protein Analysis………………………………………………………………………………31

6.1 Information Display and Function Keys……………………………………………………………31
6.2 Setting Parameters……………………………………………………………………………………..31
6.2.1 Setting DNA/Protein analysis method……………………………………………………………32
6.2.2 Setting measuring wavelength…………………………………………………………………….32
6.2.3 Setting DNA factor……….……………………………………………………………………….33
6.2.4 Setting Protein factor……………………………………………………………………………..33
6.2.5 Setting background calibration at 320nm…………………………………………………………33
6.2.6 Setting calibration factor…………………………………………………………………………..34
6.3 Quitting Parameter Setting of DNA/Protein……………………………………………………………34
6.4 Auto Zeroing…………………………………………………………………………………………….34
6.5 Measuring Operation……………………………………………………………………………………34
6.6 Looking Up The Measured Results……………….……………………………………………………34
6.7 Printing Out……………………………………………………………….……………………………34
6.8 Quitting Measurement…………………………………………………………………………………34
Chapter 7 Utilities………………………………………………………………………………35
7.1 Information Display and Function Keys…………………….………………………...………………. 35
7.2 Setting Lamp Changeover Wavelength…………………………………………………………………35
7.3 Setting Spectral Bandwidth……………………………………………………………………………..36
7.4 Setting W Lamp On/ Off………………………………………………………………………………36
7.5 Setting D2 Lamp On/Off………………………………………………………………………………..36
7.6 Setting time……………………………………..……………………………………………………….36
7.7 Dark Current Adjustment………………………………………………………………………………..37
7.8 Wavelength Calibration (Wavelength Adj)……………………………………………………………...37
7.9 Quitting Utilities Mode………………………………………………………………………………….37
Chapter 8 Maintenance……………………………………………………………………………………………38
8.1 Points for attention…………………………………………………………………………….…………38
8.2 Daily Care……………………………………………………………………………………….……….38
8.3 Trouble shooting and maintenance……………………………………………………………….……...38
8.3.1 Diagnoses of initialization…………………………………………………………………….……38
8.4 Light Source Replacement………………………………………………………………………….…...39
8.4.1 Replacement of the W lamp…………………………………………………………………….…..40
8.4.2 Replacement of the D2 lamp……………………………………………………………………..…40
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1.Installation
1.1 Inspection of Parts

When unpacking, check with the packing list to confirm that all the items listed are included. Should any shortage
or damage occur, contact the seller immediately. Table 1-1 shows the standard contents of the instrument, which
are subject to change without notice. Please see the packing list for reference.
Table 1-1 Standard Contents

No. Part No. Description Qt.
01 Spectrophotometer main unit 1
02 Quartz cells 1 pair
03 Tungsten halogen lamp 2
04 Plus screwdriver 2
05 Plain screwdriver 2
06 Fuse tube (2A, 0.5A) 2 pcs./each
07 Power cord 1
08 Power Connector 1
09 Instruction Manual 1
10 Packing List 1
1.2 General Description of the Spectrophotometer

1.2.1 Front side of the instrument
(1) LCD display
The 320×240 dots high resolution LCD, which allows users to adjust the brightness (the brightness adjustor is
on the top left corner of the keyboard) , is used to display measurement information, parameters, data and
spectrograms.
(2) Keyboard
The 27 soft-touch keys on the keyboard are used for instrument control and operation.
(3) Sample compartment
Automatic 8-cell holder to hold the samples.
1.2.2 Back side of the instrument

(1) Light source housing
The halogen lamp (for the visible region) and the deuterium lamp (for the ultraviolet region) are installed in the
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light source housing. The lamps switch over automatically according to the wavelength range while measuring.
The lamps will turn hot when they are lit. Be careful not to be burnt when lamp replacement is made.
(2) Printer interface
Used to connect with HP 600 series of jet printer.
(3) RS-232 interface
RS-232 interface is for serial communication.
(4) Power Switch
AC power outlet is used to connect to the 110-240V, 50-60Hz main power.
Fuse holder is to hold a 1A-fuse tube of 5mm.
Power switch is to turn on/off the power of the instrument.
(5) Light source housing cover
It is for access to the light source for the replacement. Be careful not to be burned by the heated lamps when
replacing the light sources.
(6) Ventilating fan
The opening of the fan is the outlet for ventilation and dissipating heat. Please place the main unit at least
20-30cm away from the wall in case that the outlet is blocked.
1.2.3 Display
The display of SpectroScan 80D is the 320×240 dots high resolution LCD that can not only display the
measurement data, parameters, mode and man-machine interaction information, but also the spectrum measured.
The display is shown as follows:
Indicating Area For
Mode Line
Function Keys
Information
Indicating Area
Operating
Time
Area

The first line is the mode line, which is used to indicate current working mode. The system provides four working
modes:
Photometric Measurement – Used to make measurement at fixed wavelengths. K-Factor calculation is
possible.
Spectrum Measurement – Used to provide a wavelength scan and data processing in the set wavelength
range.
Quantitative measurement – Used to provide one wavelength quantitative determination and data
processing.
DNA/Protein analysis – Used to provide 3 measurement methods for the analysis of DNA/Protein.
Utilities – Used to set the parameters for the instrument system.
The column on the right of the screen is the indicating area for function keys (F1~F4). The function of each
function key may vary from page to page.
In the middle of the screen is the operating area.
At the bottom of the screen is the information indicating area, which allows you to enter parameter values
there.
1.2.4 Keyboard
There are altogether 27 soft-touch keys on the keyboard of SpectroScan 80D, which are divided into 4 groups.
The functions of the keys are described as follows:
(1) Function keys (F1~F5)

The function keys are used to set instrument parameters and to perform operation functions. When one of the
four function keys (F1~F5) is pressed in different working mode, the system executes the corresponding
operation, such as parameter setting, sample cell setting and printing etc..
(2) Numeric keys (0~9, “.” and “-”)
Used to enter a numerical value in parameter setting.
(3) Edit keys ( ▲ , ▼ , , , , RETURN)
Cursor keys (▲ ,▼ ): Used to shift the cursor up and down while browsing data and increase or decrease
data while setting time.

Cursor keys ( , , ): Used to switch the displayed data while measuring DNA/Protein and switch
the objects while setting time.
Cancel key [ ]: Used to clear off the preceding entries or cancel a certain operation.
Enter key [ ]: Used to confirm and execute the current function, or to end the parameter setting and
quit.

Return key [RETURN]: Used to quit the current function and return to the preceding page.
(4) Operation keys (AUTOZERO, GOλ , START/STOP)
Auto zero key [AUTOZERO]: Used to set ABS zero (transmittance 100%). In photometric mode and the
quantitative mode, [AUTOZERO] set ABS zero only at current wavelength. In spectrum mode,
[AUTOZERO] performs baseline calibration in the set wavelength range.
Wavelength set key [GOλ ]: Used to set the current wavelength.
Start/Stop key [START/STOP]: Used to start or stop a measurement.

1.3 Site Requirement
To ensure a better working quality and to prolong the service life of the instrument, install the instrument in a
place that satisfies the following requirements:
a. Ambient temperature: 15℃~35℃
b. Relative humidity: less than 80%

c. The instrument should be placed on a stable workbench, avoiding strong vibrations.
d. Avoid a place with strong magnetic or electric fields and corrosive gases.
e. Power requirements: AC110V±10%, 60Hz±1Hz, single phase. A regulated power supply with at least
500VA capacity is recommended. There should be a ground line in the laboratory to ensure the
instrument to be well grounded.
1.4 Installation
SpectroScan 80D Spectrophotometer consists of the spectrophotometer main unit and a HP jet-printer (optional).
The connections between them are shown in the following figure. Item (1) and (2) are the power cords which
should not be connected to the main power until other connections are made, to avoid instrument damage. Item (3)
is the printing cable, one end of which with a 25-pin plug is connected to the main unit, the other end with a 36-pin
plug is connected to the printer.
Connect all the cables firmly by locking the screw (if there is any) on the plugs. After checking to ensure that the
power supply is AC220V, 50Hz, plug in the power cords to connect the spectrophotometer and the printer to the
main power.
Note: The spectrophotometer is a precision electronic instrument. Don’t pull out or insert the power cable or
tie cable when the instrument is on, otherwise it will destroy the instrument and the computer.
1.5 Turning on the Instrument

After confirming that there is no light shield in the sample compartment, turn on the power switch of the main unit
and then the printer (if there is). The initializing page will be displayed on the LCD. The instrument starts
self-checking and initializing. This process will take about 3 minutes. After initializing, the system displays the
instrument main menu.
Users can select a working mode by depressing the corresponding numeric keys (1-5). It usually takes 15~30
minutes for the instrument to warm up before measurement can be started.
After measurement, you should first turn off the main unit, then the printer, and at last other equipment (such as the
regulated power supply).
1.6 Performance Check at Installation

After finishing the installation, check each of the following items. Periodic checking should also be made during
operation. Proceed the performance check according to the following procedure 30 minutes after turning on the
power switch.
1.6.1 Wavelength accuracy and reproducibility
The wavelength accuracy should be within ±0.5nm. The wavelength reproducibility should be within 0.2nm.
Check with the two spectral lines of the D2 lamp according to the following procedure.
0) Confirm the spectral bandwidth is set to 2nm. The initialized spectral bandwidth is 2nm. Please refer to
THE UTILITIES FUNCTION for details of setting the spectral bandwidth.
1) Press [2] key to select mode “Spectrum Measurement” on the main menu.
2) In Abs or %T mode, press [GOλ ] to set the wavelength to 330nm, to turn to the D2 lamp working mode.
3) Press [F1] key to select parameter setting function. Set the following parameters: Sampling interval: 0.2nm;
Scanning speed: middle; Wavelength range: 660~480nm; Recording range: 0~100; Mode: Ess.
4) Press [START/STOP] key and set the energy gain value to 5~7, to start scanning.
5) Press [F2] key and set the min. peak/valley value to 1~10, to perform the peak-picking. Press [F4] to print out.
Repeat scanning for three times and perform peak-picking and printing out. Compare the picked wavelengths with
the standard wavelengths of the two peaks of D2 lamp: 656.1nm and 486.0nm.
1.6.2 Baseline flatness

With air in sample cell, follow the procedure below to measure the Abs value at whole wavelength range in

spectrum mode:
0) Confirm that spectral bandwidth is set to 2nm. The initialized spectral bandwidth is 2nm. Please refer to
THE UTILITIES FUNTION for details of setting the spectral bandwidth.
1) Press [2] key to select mode “Spectrum Measurement” on the main menu.
2) Press [F1] key to select parameter set function. Set the following parameters: Sampling interval: 1nm;
Scanning speed: middle; Wavelength range: 1100~190nm; Recording range: -0.1~0.1; Photometric mode:
Abs.
3) Press [AUTOZERO] to start baseline correction.
4) Press [START/STOP] to start scanning.
5) Press [F4] key to print out the spectrum.
Compare the max. Abs value on the spectrum with the value required for the baseline flatness. (Sudden change in
Abs value is allowed at the point of light source changeover.)

2.Instrument Specifications
Model SpectroScan 80D SpectroScan 80DV

Wavelength range 190nm~1100nm
Spectral bandwidth 2nm 5, 2, 1, 0.5nm (4 steps)
Resolution 2nm 0.5nm
Wavelength display 0.1nm
1nm increment for scan starting and ending wavelengths setting.
Wavelength setting
0.1nm increment for GOTO-λ setting.
Wavelength accuracy ±0.5nm (with automatic wavelength correction)
Wavelength reproducibility ±0.2nm
Photometric system Double beam ratio monitoring system
±0.002Abs (0~0.5Abs)
Photometric accuracy
±0.004Abs (0.5~1.0Abs)
±0 3%T (0 100%T)
±0.001Abs (0~0.5Abs)
Photometric reproducibility
±0.002Abs (0.5~1.0Abs)
±0 15%T (0 100%T)
Stray light ≤ 0.3%T (220nm)
100%T Noisy ≤ 0.3%T(at 500nm, 2nm bandwidth, for 2 minutes)
Baseline flatness ±0.004Abs (2nm bandwidth , middle scanning ,1100~190nm)
Stability ≤ 0.002A/30min (2 hours warming up, 2nm bandwidth, at 500nm)

3.Photometric Measurement
In photometric mode, one-wavelength measurements of absorbance and transmittance can be made. K-Factor
calculation and printing out are possible.
3.1 Information Display and Function Keys

On the instrument main menu , press [1] key to select photometric measurement mode. The screen of photometric
mode is displayed on LCD, as shown in Fig. (3-1).
Fig.(3-1) The working picture of photometric measurement

The first line on the menu indicates that the current working mode is photometric mode.
In the operating area displays the current measuring wavelength (660.0nm) and the measured value (0.001Abs).
On the right, the functions of F1-F4 keys are displayed.
[F1] - parameter setting
[F2] – deleting the measurement data and refresh the screen
[F3] –setting cell holder
[F4] - printing the measurement results
On this page, you can also set the wavelength and execute auto-zero by depressing [GOλ ] key and [AUTOZERO]
key respectively.
3.2 Parameter Setting

On the working picture of photometric measurement, press [F1] key to display the parameter setting page, as
shown in Fig.(3-2).

Fig. (3-2) Parameter setting page of photometric measurement
On this page, users can set the photometric mode, wavelength and the value of factor K by pressing the
corresponding item No.. After finishing parameter setting, press [RETURN] key to return to the preceding page.
3.2.1 Setting photometric mode

Press [1] key to set photometric mode. Two modes are available: Abs and %T. They can be selected alternately
each time when [1] key is pressed.
Fig.(3-3) Mode setting page in photometric measurement

3.2.2Setting Measuring Wavelength
Press [2] key to display the measuring wavelength setting page in photometric measurement. The indicating
message indicates users to input the measuring wavelength, as shown in Fig. (3-4).
Fig. (3-4) Wavelength setting page in photometric measurement

At the bottom of this page, enter desired wavelength value by pressing the numeric keys (0~9) and decimal point
(.). The CE key is used to clear off the preceding entry. Press [ENTER] key to confirm the numeric entry and quit.
If nothing is to be entered, press [RETURN] key to quit this page directly. A 5-digit figure entry is allowed. The
range is from 190 to 1100. The input value keeps one-digit floating number after the decimal point If the figure
entered exceeds five digits, the system will delete what has been entered automatically and you should start a new
entry again.
3.2.3 Setting K-Factor

Press [3] key to display K-factor setting page (Fig. 3-5) in photometric measurement. The indicating line at the
bottom of the page indicates users to enter factor K value.
Article I. Fig. (3-5) K-Factor setting page in

photometric measurement
At the bottom of the page, set the K-Factor value by pressing the numeric keys (0~9), [.] and [-] key. [CE] key is
used to clear off the preceding entry. Press [ENTER] key to confirm the numeric entry and quit. If nothing is to be
entered, press [RETURN] key to quit this page directly. A 4-digit figure (0~±9999) entry is allowed. If the figure
entered exceeds four digits, the system will delete what has been entered automatically. You should start a new
entry.
3.2.4 Quitting parameter setting

Press [RETURN] key to quit parameter setting and return to the main menu of photometric measurement.
3.3 Setting Working Wavelength
On the main menu of photometric measurement, press [GOTO-λ ] key to display the following page (Fig. 3-6) and
set the working wavelength for photometric measurement.
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Fig. (3-6) Wavelength setting page in photometric measurement

At the bottom of this page, enter the wavelength value by pressing the numeric keys (0~9) and decimal point (.).
The CE key is used to clear off the preceding entry. Press [ENTER] key to confirm the numeric entry and quit. If
nothing is to be entered, press [RETURN] key to quit this page directly. A 5-digit figure entry is allowed. The range
is from 190 to 1100. If the figure entered exceeds five digits, the system will delete what has been entered
automatically and you should start a new entry again.
3.4 Auto-zeroing (100%T setting)

On the main menu of photometric measurement, press [AUTOZERO] key to set Abs zero automatically at the
current measuring wavelength. Before starting auto-zeroing, put the blank sample into the sample compartment.
3.5 Sample Control
On the main menu of photometric measurement, press [F3] key to display the sample control page as shown in Fig.
(3-7).
Fig. (3-7) Sample control page in photometric measurement

Users can select sample cell-holder type (fixed cell-holder, 5-cell holder and 8-cell holder) in the sample control
page. If the 8-cell holder is selected, you can set the working parameters of the 8-cell holder by pressing the
corresponding numeric keys. After finishing the parameter setting, press [RETURN] key to return to the preceding
page.
3.5.1 Selecting sample module type

Press [1] key to select sample module type. There are three types available, fixed cell-holder, 5-cell holder and
8-cell holder, which can be selected alternately each time [1] key is pressed.
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3.5.2 Setting sample cell number in use (Drive Cell No.)

This parameter is only available to 5-cell holder and 8-cell holder. Press [2] key to enter the setting page (as shown
in Fig.3-8). The system will indicate you to enter the number of sample cell in use (Drive Cell No.).
At the bottom of this page, enter the sample cell number by pressing the numeric keys (0~9). [CE] key is used to
clear off the preceding entry. Press [ENTER] key to confirm the numeric entry and quit. A 1-digit figure entry is
allowed. The range is from 1 to 8. If the entered figure exceeds the range, the system will delete what has been
entered. You should start a new entry.
In the process of measurement, the system will measure the samples in the set sample cells one by one. The serial
numbers of the sample cells are expressed by n-1, n-2,….
Fig. (3-8) Sample cell number setting page

3.5.3No. 1 Cell blank correction
This parameter is used to set No. 1 cell blank calibration function which means to calibrate the measuring result of
the sample in each sample cell (No. 2-8) with that of the blank in the No. 1 cell. In measurement, the system will
deduct the measured value of the blank in No. 1 cell from that of the samples in the same cell (No.2 ~ No.8), the
result of which will be the measuring result. Two choices are available for this item: YES and NO, which alternate
with each other each time [3] key is pressed.
3.5.4 Sample cell shifting (Move Cell)

Press [4] key to shift the cell holder to the next sample cell.
3.5.5 Resetting to cell No.1(Move Home)

Press [5] key to reset the cell holder to sample cell No.1.
3.5.6 Single-cell measurement

If the single-cell type is selected, you can choose any of the cells of the multi-cell as the measuring cell by shifting
the cell holder to the desired position according to the method described in 3.5.6. Measurement will only be done
to the chosen cell.
3.5.7 Quitting sample control

When sample control operation is finished, users can press [RETURN] key to quit sample control function and
return to the main menu of photometric measurement.
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3.6 Measuring Operation

On the main menu of photometric measurement, press [START] key to start the measurement (Fig. 3-9).
Fig. (3-9) Operating page of photometric measurement

In the table shown on the screen, “NO.” means the sample No. set by users, which increases by one after each
measurement. “Abs” is the measured value Column. “K·Abs” is the value of the measurement multiplied by
K-Factor. In this page, press [START/STOP] key to start measurement. Press [F2] key to delete all the measuring
data. Press [F4] to print out the measuring data. After finishing all the measurement operation, press [RETURN]
key to return to the main menu of operation.
3.6.1 Setting measuring wavelength
Press [GOλ ] key to set measuring wavelength. Please see Section 3.3 for the details of measuring wavelength
setting.
3.6.2 Auto zeroing (100%T calibration)
Press [AUTOZERO] key to set Abs zero at the current wavelength. Please see Section 3.4 for the details of
auto-zeroing (100%T calibration).
3.6.3 Starting measurement

After finishing parameter setting and Abs auto-zeroing, press [START/STOP] key to start measurement. Each time
[START/STOP] is pressed, a measured value is obtained and calculation of the measured value multiplied by
K-Factor is done. Both the measured value and the calculated value are saved in the control unit and displayed
sequentially in the table on the LCD. When each measurement is finished, the sample number will increase by 1.
The memory of the control unit can store up to 500 groups of measured data.
The measuring process and the measured results may vary with the different settings in Section 3.5:
(1) When Reagent Blank Correction in Cell No. 1 is set to “NO”, the system will start measurement to the set N
samples (refer to§3.5.2). Then the measured value will be calculated, saved and displayed on LCD one by
one.
(2) When Reagent Blank Correction in Cell No. 1 (§3.5.3) is set to “YES”, the system will first measure the set N
samples (refer to §3.5.2). Then the measured values of samples No.2~No.N will be deducted (corrected) by
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that of the blank in sample cell No. 1 following the formula below. The corrected results will be recorded,
calculated and displayed on LCD one by one.
Formula of deduction (correction):
The absorbance of sample No.n = measured value of sample No.n – measured value of sample No.1(blank)
The transmittance of sample No.n = measured value of sample No.n / measured value of sample No.1(blank)
× 100%
3.6.4 Looking up the measured results
During measurement, users can look up the measured results line by line, by pressing the cursor keys [▲ ],[▼ ] .
3.6.5 Clearing off the measured data

During measurement, users can clear off the measured data at any time when [F2] key is pressed.
Fig. (3-10) Data deleting page in photometric measurement

3.6.6 Printing out
During measurement, users can print out the measured results in the memory at any time when [F4] key is pressed.
Article II. Fig. (3-11) Data printing page in

photometric measurement
3.6.7 Quitting measuring operation
After measurement, press [RETURN] key to quit measuring operation. The system indicates that the data will be
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lost. Users can press [RETURN] key to return to the main menu of photometric measurement.
4.Spectrum Scanning
This mode provides a wavelength scan and print out for absorbance, transmittance and energy of the measured
sample.

On the instrument main menu (mode menu), press [2] key to select spectrum scanning mode. The main menu of
spectrum scanning mode is displayed on LCD, as shown in Fig. (4-1).
Fig.(4-1) The main menu of spectrum scanning

The first line on the menu indicates that the current working mode is spectrum scanning.
In the upper part of operating area displays the current working wavelength (660.0nm) and the measured value
(0.000Abs), while in the lower part is a coordinate diagram.
On the right, the function of each function key is displayed.
[F1] – parameter setting
[F2] – peak-picking
[F3] – sample control
[F4] – spectrum printing
On this page, wavelength setting, baseline correction and spectrum scanning can be done by depressing [GOλ ]
key, [AUTOZERO] key and [START/STOP] key respectively.

On the main menu of spectrum scanning, press [F1] key to display the parameter setting page, as shown in Fig. (4-2).
15

Fig. (4-2) Parameter setting page of spectrum scanning
On this page, users can set the photometric mode, scanning speed, scanning wavelength range, ordinate full scale
range and sampling interval by pressing the corresponding numeric keys. After parameter setting is finished, press
[RETURN] key to return to the preceding page.
4.2.1 Setting photometric mode

Press [1] key to set photometric mode. Four modes are available: Abs, Er, Es and %T , which can be selected in
turn when [1] key is pressed. Thereinto Es and Er are the energy measuring on the reference beam and the sample
beam respectively. When measuring the energy of the light beam, the energy gain will be asked to input first , and
then the system executes a spectrum scan.
4.2.2 Setting scanning speed

Press [2] key to set the scanning speed to Fast, Middle or Slow in turn.
4.2.3 Setting sampling interval

Press [3] key to set sampling interval. You have 5 choices: 1nm, 2nm, 0.1nm, 0.2nm or 0.5nm, which can be
selected in turn when [3] key is pressed.
A maximum of 1100 points can be stored for one spectrum, because the memory space of microprocessor is limited.
If the set wavelength range is wider than 110nm and the sampling interval is set to 0.1nm, the system will change it
to 0.2nm automatically. In other circumstances, the setting will not be changed.
4.2.4 Setting wavelength range
Press [4] key to display the wavelength range setting page of spectrum scanning. The system indicates user to set
wavelength values, as shown in Fig. (4-3).
16

Fig. (4-3) Wavelength range setting page of spectrum scanning
At the bottom of the page, first set the starting wavelength (long wave) by pressing numeric keys (0~9). [CE] key
is used to clear off the preceding entry. After setting the starting wavelength, the system indicates user to set the
ending wavelength (short wavelength). Press [ENTER] key to confirm the numeric entry and quit. If nothing is to
be entered, press [RETURN] key to quit directly. A 5-digit figure entry is allowed. The range is from 190 to 1100.
If the figure entered exceeds five digits, the system will delete what has been entered. You should start a new entry.
If the ending wavelength value entered is larger than that of the starting wavelength, an error message will be
displayed. You should enter it again.
4.2.5 Setting ordinate full scale range

Press [5] key to display the ordinate (measured value) full scale range setting page shown as Fig. (4-4).
Fig. (4-4) Ordinate scale range setting page
At the bottom of the page, the system indicates user to set the upper limit of the ordinate. Press numeric keys (0~9),
(.) and (-) to enter the desired value. [CE] key is used to clear off the preceding entry. After setting the upper limit,
the system indicates user to set the lower limit. Press [ENTER] key to confirm the numeric entry and quit. If
nothing is to be entered, press [RETURN] key to quit directly. A 4-digit figure entry is allowed. The range is from
–9999~9999. If the figure entered exceeds four digits, the system will delete what has been entered. You should
enter it again.
17

4.2.6 Dark Current Adjustment

Press [F5 ] key to carry out dark current adjustment. The dark current adjustment is to be done, as shown in Fig.
(4-5).
Fig. (4-5) Dark current calibration page
4.2.7 Quitting parameter settings

Press [RETURN] key to quit parameter setting operation and return to the main menu of spectrum scanning.
4.3 Setting Measuring Wavelength
On the main menu of spectrum scanning, press [GOλ ] key to display the following page (Fig. 4-6) and set the
measuring wavelength for spectrum scanning.
Fig. (4-6) Wavelength setting page for spectrum scanning
At the bottom of this page, enter the desired wavelength value by pressing the numeric keys (0~9), [.]. [CE] key is
entered, press [RETURN] key to quit directly. A 5-digit figure entry is allowed. The range is from 190 to 1100. If
the figure entered exceeds five digits, the system will delete what has been entered. You should start a new entry.
4.4 Baseline Correction

On the main menu of spectrum scanning, press [AUTOZERO] key to start baseline correction at the set wavelength
18

range. Before starting baseline correction, put the blank sample into the sample compartment. [START/STOP] key
can be pressed to stop baseline correction operation. If the wavelength range has been changed, the baseline
calibration should be done again.

On the main menu of spectrum scanning, press [START/STOP] key to start spectrum scanning measurement. The
measured spectrum will be displayed dynamically, as shown in Figure 4-7. When the scan is to be stopped, press
[START/STOP] key again.
Fig. (4-7) Spectrum scan operatin

A maximum of 1100 points can be stored for one spectrum, because the memory space of micro-processor is
limited,. If the set wavelength range is wider than 110nm and the sampling interval is set to 0.1nm, the system will
change it to 1nm automatically. In other circumstances, the setting will not be changed.
4.6 Peak-picking
On the main menu of spectrum scanning, press [F2] key to display peak-picking operating page as shown in Fig. (4-8).
Fig. (4-8) Peak-picking operating page

At the bottom of this page, enter the minimum height value of picked peaks/valleys by pressing the numeric keys
(0~9), [.]. [CE] key is used to clear off the preceding entry. Press [ENTER] key to confirm the numeric entry and
quit. If nothing is to be entered, press [RETURN] key to quit directly. A 4-digit figure entry is allowed. If the figure
entered exceeds four digits, the system will delete what has been entered. You should start a new entry.
After the minimum peak/valley value is entered, the system will start peak-picking calculation and the calculated
19

result will be displayed on the screen. The peaks and valleys on the spectrum are marked. At the same time, an
arrow indicates the current peak/valley point. Below the spectrum, the wavelength and the measured value of the
pointed peak/valley are displayed. By pressing the [ ]or [ ] key, the arrow will be shifted to look up other
peak/valley points. Press [F4] key to print out the result. Press [RETURN] key to return to the main menu of the
spectrum scanning after all the operations are ended.
4.7 Printing Out the Measured Result

When spectrum scanning is finished, press [F4] key to print out the measured spectrum. [F4] key will not be
effective if there is no spectrum stored.
Figure 4-9 spectrum printing page

Press [ENTER] key to print the spectrum or press [CE] key to quit printing.
4.8 Quitting Spectrum Scanning Measurement

When spectrum scanning measurement operation is finished, press [RETURN] key to quit spectrum scanning. The
system indicates that the data will be lost. Users can press [ENTER] key to return to the main menu of the
instrument.
20

5. Quantitative Measurement
In this mode one-wavelength determination can be done. Both K-Factor Method and Calibration Curve Method are
used in quantitative measurement.

On the instrument main menu, press [3] key to select the quantitative measurement mode. The main menu of
quantitative measurement will be displayed as shown in Fig. (5-1).
Fig. (5-1) The main menu of quantitative measurement
The first line on the menu is the mode line, which shows that the current operation mode is quantitative
measurement.
The upper part of the operating area shows the current measuring wavelength (500.0nm) and the measured value
(0.000Abs), the lower part is a table in which the measured data are displayed.
The right part of the page is the function key indicating area, which shows the functions of function keys [F1]
~[F4].
[F1] – parameters setting
[F2] – clearing off the measurement data
[F3] – sample control
[F4] – printing
On this page, the measuring wavelength setting, auto-zeroing and sample measurement can be carried out by
depressing [GOλ ] key, [AUTOZERO] key and [START/STOP] key respectively.

Parameter setting page (as shown in Fig. (5-2)) can be displayed by pressing [F1] key on the main menu of
quantitative measurement.
21

Fig. (5-2) Parameter setting page of quantitative measurement

On this page, the measurement method, measuring wavelength and concentration unit can be set directly by
pressing the numeric keys [1] ~ [3]. By pressing [F1] key the second time the calibration curve setting page can be
displayed. After finishing all parameter settings, press [RETURN] key to return to the preceding page.
5.2.1 Setting measurement method

Press [1] key to set measurement method for quantitative measurement. There are two selections: K-Factor method
and Calibration Curve method. The two methods will be selected alternately when [1] key is pressed each time.

Press [2] key to display the wavelength setting page of quantitative measurement. The system will indicate users to
enter the desired wavelength value, as shown in Fig. (5-3).
Fig. (5-3) Wavelength setting page of quantitative measurement
entered, press [RETURN] key to quit directly. A 5-digit figure entry is allowed. The range is from 190 to 1100. If
the figure entered exceeds five digits, the system will delete what has been entered. You should start a new entry.
5.2.3 Setting concentration unit

Press [3] key to display concentration unit setting page as shown in Fig.(5-4). The system will indicate users to
select concentration unit. There are 8 choices: %, mg/l, ug/l, g/l, mg/ml, ng/ml, mol/l and no. The 8 units will be
22

selected alternately when [3] key is pressed each time.

5.2.4 Setting calibration curve parameters (K-Factor method)
Press [F1] key on parameter setting page of quantitative measurement to display the calibration curve parameter
setting page. The content displayed on the page will vary with the method selected previously in Section 5.2.1.
When K-Factor method is selected, the page shown in Fig. (5-4) will be displayed.
Fig. (5-4) Calibration curve parameter setting page for K-Factor method
Press [1] key and [2] key to enter slope and intercept values of the calibration curve on this page. Press [F4] key to
print out the parameters currently displayed on the screen. After all parameter settings are completed, press
5.2.4.1 Setting the slope of calibration curve

Press [1] key to display the slope setting page, as shown in Fig. (5-5).
Fig. (5-5) Slope setting page
At the bottom of this page, enter the slope value by pressing the numeric keys (0~9), [.]. [CE] key is used to clear
off the preceding entry. Press [ENTER] key to confirm the numeric entry and quit. If nothing is to be entered, press
[RETURN] key to quit directly. A 4-digit figure entry is allowed. The range is from 0 to 9999. If the figure entered
exceeds four digits, the system will delete what has been entered. You should start a new entry. When a decimal
number is entered, a “0” should be put before the decimal point, such as 0.12.
23

5.2.4.2 Setting the intercept of calibration curve

Press [2] key to display the intercept setting page, as shown in Fig. (5-6).
Fig. 5-6 Intercept setting page
At the bottom of this page, enter the intercept value by pressing the numeric keys (0~9), [.] and [-]. [CE] key is
entered, press [RETURN] key to quit directly. A 4-digit figure entry is allowed. The range is from 0 to 9999. If the
figure entered exceeds four digits, the system will delete what has been entered. You should start a new entry.
5.2.4.3 Quitting calibration curve parameter setting

Press [RETURN] key to quit calibration curve parameter setting and return to the parameter setting page of
5.2.5 Setting calibration curve parameters for Calibration Curve method

On parameter setting page of quantitative measurement, press [F1] key to enter the calibration curve parameter
setting page. The content displayed on the page will vary with the method selected previously in Section 5.2.1.
When Calibration Curve method is selected, the page shown in Fig. (5-7) will be displayed.
Fig.(5-7) Calibration curve parameter setting page for calibration curve method
Press numeric keys [1]~ [3] to enter the number of standard samples, the concentration and absorbance values (if
they are known) of the standard, and to carry out absorbance measurement to the standard. After finishing
24

parameter setting, press [F3] key to set up calibration curve. After all parameter settings are completed, press
5.2.5.1 Setting the number of standards

Press [1] key to display standard number setting page as shown in Fig. (5-8), at the bottom of which a message is
displayed to indicate users to enter the number of standards.
Fig. (5-8) Standard number setting page
At the bottom of this page, enter the number of standards by pressing the numeric keys [1]~[8]. [CE] key is used to
allowed. The range is from 1 to 8. If the figure entered exceeds 1 digit, the system will delete what has been
5.2.5.2 Entering concentrations and absorbance of standards

Press [2] key to display concentration and absorbance setting page. The system will indicate users to enter the
concentration and absorbance values of standard No. First, the system indicates users to enter the concentration, as
shown in Fig. (5-9).
Fig. (5-9) Concentration and absorbance setting page
At the bottom of this page, enter the concentration value by pressing the numeric keys (0~9), [.]. [CE] key is used
to clear off the preceding entry. After the concentration is entered, the system will indicate users to enter the
absorbance values. Press [ENTER] key to confirm the numeric entry and quit. If nothing is to be entered, press
[RETURN] key to quit directly. A 4-digit figure entry is allowed. The range is from 0 to 9999. If the figure entered
25

exceeds four digits, the system will delete what has been entered. You should start a new entry. When a decimal
number is entered, a “0” should be put before the decimal point, such as 0.5.
Press [2] again and repeat the above procedure if concentration and absorbance of other standards are to be entered.
5.2.5.3 Starting standard sample measurement

Press [3] key to display the standard sample measurement page as shown in Fig. (5-10). The system indicates that
sample No. N to be measured should be placed in Cell No.1.
Fig. (5-10) Standard sample measurement page

After the standard sample is placed well in the cell, press [START/STOP] key to start measurement. The measured
result will be displayed on LCD. Users can repeat measurement to the same sample for several times, but only the
last measured result is saved. After the measurement is finished, press [RETURN] key to quit.
Press [3] key again, the standard number increases by 1. If the standard number exceeds the set number of standard,
the standard number will return to the first standard (No. 1).
5.2.5.4 Building the calibration curve

After the data of standard samples are entered and measurement is completed, press [4] key to carry out curve
fitting and set up the calibration curve. Parameters of the calibration curve will be displayed on LCD as shown in
Fig. (5-11).
Fig. (5-11) Calibration curve set-up page

Press [RETURN] to return to calibration curve parameter setting page.
26

5.2.5.5 Printing out the calibration curve

Press [F4] to print out parameters of the calibration curve in the format displayed on LCD. The system indicates if
the printer is OK or not. If the printer is OK, press [ENTER] key to print it out.
5.2.5.6 Quitting calibration curve parameter setting

Press [RETURN] key to quit calibration curve parameter setting, and return to parameter setting page in
5.2.6 Quitting parameter setting of quantitative measurement

Press [RETURN] key to quit parameter setting of quantitative measurement and return to the main menu of
5.3 Auto Zeroing

On the main menu of quantitative measurement, press [AUTOZERO] key to set Abs zero automatically at current
measuring wavelength. Before starting auto zeroing, put the blank sample into the cell.
5.4 Setting Measuring Wavelength
Press [GOλ ] key to set the measuring wavelength on the main menu of quantitative measurement, which is shown
in Fig. (5-12).
Fig. (5-12) Wavelength setting page in quantitative measurement
entered, press [RETURN] key to quit this page directly. A 4-digit figure entry is allowed. The range is from 190 to
1100. If the figure entered exceeds four digits, the system will delete what has been entered automatically. You
should start a new entry.
5.5 Sample Control

On the main menu of photometric measurement, press [F3] key to display the sample control page as shown in Fig.
(5-13).
27

Fig. (5-13) Sample control page in photometric measurement

Users can select sample cell-holder type (fixed cell-holder, 5-cell holder and 8-cell holder) in the sample control
page. If the 8-cell holder is selected, you can set the working parameters of the 8-cell holder by pressing the
corresponding numeric keys. After finishing the parameter setting, press [RETURN] key to return to the preceding
page.
5.5.1 Selecting sample module type

Press [1] key to select sample module type. There are three types available, fixed cell-holder, 5-cell holder and
8-cell holder, which can be selected alternately each time [1] key is pressed.
5.5.2 Setting sample cell number in use (Drive Cell No.)

This parameter is only available to 5-cell holder and 8-cell holder. Press [2] key to enter the setting page (as shown
in Fig.5-14). The system will indicate you to enter the number of sample cell in use (Drive Cell No.).
At the bottom of this page, enter the sample cell number by pressing the numeric keys (0~9). [CE] key is used to
allowed. The range is from 1 to 8. If the entered figure exceeds the range, the system will delete what has been
In the process of measurement, the system will measure the samples in the set sample cells one by one. The serial
numbers of the sample cells are expressed by n-1, n-2,….
Fig. (5-14) Sample cell number setting page
28

5.5.3 No. 1 Cell blank correction

This parameter is used to set No. 1 cell blank calibration function which means to calibrate the measuring result of
the sample in each sample cell (No. 2-8) with that of the blank in the No. 1 cell. In measurement, the system will
deduct the measured value of the blank in No. 1 cell from that of the samples in the same cell (No.2 ~ No.8), the
result of which will be the measuring result. Two choices are available for this item: YES and NO, which alternate
with each other each time [3] key is pressed.
5.5.4 Sample cell shifting (Move Cell)

Press [4] key to shift the cell holder to the next sample cell.
5.5.5 Resetting to cell No.1(Move Home)

Press [5] key to reset the cell holder to sample cell No.1.
5.5.6 Single-cell measurement

If the single-cell type is selected, you can choose any of the cells of the multi-cell as the measuring cell by shifting
the cell holder to the desired position according to the method described in 3.5.6. Measurement will only be done
to the chosen cell.
5.5.7 Quitting sample control

When sample control operation is finished, users can press [RETURN] key to quit sample control function and
return to the main menu of photometric measurement.

After all measuring parameters are set and blank corrections are made, press [START/STOP] key to start
measurement. Each time [START/STOP] key is pressed, a measured Abs value is obtained and the concentration of
the measured sample will be calculated. Both the measured Abs value and the calculated concentration value will
be saved in the control unit and displayed sequentially in the table on the LCD. The sample number will increase
by 1 after each measurement. The memory of the control unit can store up to 500 groups of measured data.
The measuring process and the measured results may vary with the different settings in Section 5.5:
(1) When Reagent Blank Correction in Cell No. 1 is set to “NO”, the system will start measurement to the set N
samples (refer to§5.5.2). Then the measured value will be calculated, saved and displayed on LCD one by one.
(2) When Reagent Blank Correction in Cell No. 1 (§5.5.3) is set to “YES”, the system will first measure the set N
samples (refer to §5.5.2). Then the measured values of samples No.2~No.N will be deducted (corrected) by
that of the blank in sample cell No. 1 following the formula below. The corrected results will be recorded,
calculated and displayed on LCD one by one.
Formula of deduction (correction):
The absorbance of sample No.n = measured value of sample No.n – measured value of sample No.1 (blank)
The transmittance of sample No.n = measured value of sample No.n / measured value of sample No.1 (blank)
× 100%
29

5.7 Looking Up the Measured Results
During measurement, users can look up the measured results line by line, by pressing the cursor keys [▲ ],[▼ ] .
5.8 Printing Out

During measurement, users can print out the measured results in the memory at any time when [F4] key is pressed.
5.9 Quitting Quantitative Measurement

After finishing quantitative measurement, press [RETURN] key to quit quantitative measurement operation. When
[RETURN] key is pressed, the system will indicate that the measured data will be cleared off. Users can press
[ENTER] key to return to the main menu of the instrument operation.
30

6. DNA/Protein Analysis
In this mode DNA/Protein analysis can be done. There are three methods: Method 1, Method 2 and Self-defining.

On the instrument main menu, press [4] key to select the DNA/Protein mode. The main menu of quantitative
measurement will be displayed as shown in Fig. (6-1).
Figure 6-1 The main menu of DNA/Protein Analysis

The first line on the menu is the mode line, which shows that the current operation mode is DNA/Protein analysis.
The upper part of the operating area shows the current measuring method and the corresponding wavelengths
(500.0nm and 230.0nm) and the lower part is a table in which the measured data are displayed.
The right part of the page is the function key indicating area, which shows the functions of function keys [F1]
~[F4].
[F1] – parameters setting
[F2] – clearing off the measurement data
[F4] – printing
On this page, the autozeroing and sample measurement can be carried out by depressing [AUTOZERO] key and
[START/STOP] key respectively.
Press [ ] [ ] to change A1, A2, DNA concentration, protein concentration alternately, as shown in Figure
6-2.
Figure 6-2 Concentration display page of DNA/Protein
31

6.2 Setting Parameters

Parameter setting page (as shown in Fig. (6-3)) can be displayed by pressing [F1] key on the main menu of
DNA/Protein analysis.
Figure 6-3 Parameter setting page of DNA/Protein analysis
Press [ ] [ ] to select the measuring methods. Method 1 and Method 2 are the system defaulted parameters
that can not be changed by users. Users can set parameters according to their requirements under the self-defining
mode. After finishing all parameter settings, press [RETURN] key to return to the preceding page.
6.2.1 Setting DNA/Protein analysis method

Press [ ] [ ] to select DNA/Protein measuring methods. The selected method will be reverse displayed.

Under the custom mode, press [1] key to display the wavelength setting page of DNA/Protein analysis. The system
will indicate users to enter the desired wavelength value, as shown in Fig. (6-4).
Figure 6-4 Wavelength setting page of DNA/Protein analysis
used to clear off the preceding entry. The system will indicate users to input the second wavelength after inputting
the first one. Press [ENTER] key to confirm the numeric entry. If nothing is to be entered, press [RETURN] key to
32

quit directly. A 5-digit figure entry is allowed. The range is from 190 to 1100. If the figure entered exceeds five
digits, the system will delete what has been entered. You should start a new entry.
6.2.3 Setting DNA factor
Under the self-defining mode, press [2] key to display the DNA factor setting page. The system will indicate users
to input DNA factor, as shown in Figure 6-5.
Figure 6-5 DNA/Protein factor setting page

At the bottom of this page, enter the desired protein factor by pressing the numeric keys (0~9), [.]. [CE] key is used
to clear off the preceding entry. The system will indicate users to input the second factor after inputting the first one.
Press [ENTER] key to confirm the numeric entry. If nothing is to be entered, press [RETURN] key to quit directly.
A 5-digit figure entry is allowed. The range is from –9999 to 9999. If the figure entered exceeds five digits, the
system will delete what has been entered. You should start a new entry.
6.2.4 Setting protein factor

Under the self-defining mode, press [3] key to display the protein factor setting page. The system will indicate
users to input Protein factor, as shown in Figure 6-6.
Figure 6-6 Protein factor setting page

At the bottom of this page, enter the desired protein factor by pressing the numeric keys (0~9), [.]. [CE] key is used
to clear off the preceding entry. The system will indicate users to input the second factor after inputting the first one.
Press [ENTER] key to confirm the numeric entry. If nothing is to be entered, press [RETURN] key to quit directly.
A 4-digit figure entry is allowed. The range is from –9999 to 9999. If the figure entered exceeds five digits, the
system will delete what has been entered. You should start a new entry.
33

6.2.5 Setting background calibration at 320nm

Under the self-defining mode, press [4] key to set background calibration at 320nm. Two choices are available for
this item: YES and NO, which can be selected alternately each time [4] key is pressed.
6.2.6 Setting calibration factor
Under the self-defining mode, press [5] key to display the calibration factor setting page. The system indicate users
to input the calibration factor, as shown in Figure 6-7.
Figure 6-7 Intercept setting page

At the bottom of this page, enter the desired calibration factor by pressing the numeric keys (1~9), [.]. [CE] key is
entered, press [RETURN] key to quit directly. A 4-digit figure entry is allowed. The range is from –9999 to 9999.
If the figure entered exceeds five digits, the system will delete what has been entered. You should start a new entry.
6.3 Quitting Parameter Setting of DNA/Protein
Press [RETURN] key to quit DNA/Protein parameter setting and return to the main menu of DNA/Protein.
6.4 Auto-zeroing
On the main menu of DNA/Protein, press [AUTOZERO] key to set Abs zero automatically at current measuring
wavelengths. Before starting auto zeroing, put the blank sample into the cell.

On the main menu of DNA/Protein, press [START/STOP] key to start measurement. Each time [START/STOP]
key is pressed, a measured Abs value is obtained. The measured Abs value will be saved in the control unit and
displayed sequentially in the table on the LCD. The sample No. will increase by 1 after each measurement. The
memory of the control unit can store up to 100 groups of measured data.
6.6 Looking Up the Measured Results
During measurement, users can look up the measured results line by line, by pressing the cursor keys [▲ ],[ ▼ ] .
6.7 Printing Out

After measurement, users can print out the measured results in the memory at any time when [F4] key is pressed.
6.8 Quitting Measurement

After finishing measurement, press [RETURN] key to quit measurement. The system will indicate that the
measured data will be lost. Press [ENTER] key to return to the instrument main menu.
34

7. Utilities
In this mode, the operating parameters can be set, such as lamp changeover wavelength, spectral bandwidth,
tungsten lamp On/Off, deuterium lamp On/Off and wavelength calibration etc..

On the instrument main menu, press [5] key to select the Utilities mode. The main menu of utilities will be
displayed on LCD, as shown in Fig. (7-1).
Fig. (7-1) Main menu of utilities

The first line on the page is the mode line, which shows the current operating mode, the Utilities mode.
In the right part of the page , the function indicating area, there is no indication for function keys [F1] ~[F4]. On
this page, [GOλ ], [AUTOZERO] and [START/STOP] keys are not to be used.
7.2 Setting Lamp Changeover Wavelength

On the main menu of utilities, press [1] key to set lamp changeover wavelength, the page of which is shown in Fig.
(7-2).
Fig. (7-2) Lamp changeover WL setting page

At the bottom of this page, enter the desired wavelength value by pressing the numeric keys (0~9). [CE] key is
35

entered, press [RETURN] key to quit this page directly. A 4-digit figure entry is allowed. The range is from 190 to
1100. If the figure entered exceeds four digits, the system will delete what has been entered automatically. You
should start a new entry.
7.3 Setting Spectral Bandwidth
Press [2] key to set the spectral bandwidth of the instrument. Four options are available: 2.0, 5.0, 0.5, 1.0nm. They
can be selected in turn when [2] key is pressed each time.
7.4 Setting W Lamp On/Off

Press [3] key to display tungsten lamp on/off setting page. On and Off can be changed in turn when [3] key is
pressed each time.
7.5 Setting D2 Lamp On/Off

Press [4] key to display deuterium lamp on/off setting page. On and Off can be changed in turn when [4] key is
pressed each time.
7.6 Setting Control mode

Press [5] key to change control mode between PC mode and MCU mode.
7.7 Setting time
Press [6] key to display time setting page. Press [▲ ] and [▼ ] to set time. Press [ ][ ] to set minute, hour,
day, month and year, as shown in Fig. (7-3).
36

Fig. (7-3) Time setting page
7.8 Dark Current Adjustment

Press [7 ] key to carry out dark current adjustment, as shown in Fig. (7-4).
Fig. (7-4) Dark current adjustment page
7.8 Wavelength Calibration (Wavelength Adj)

Press [8] key to carry out wavelength calibration function, as shown in Fig. (7-5).
Fig. (7-5) Wavelength calibration page
7.9 Quitting Utilities Mode

Press [RETURN] key to quit utilities mode and return to the instrument main menu
37

8. Maintenance
SpectroScan 80D Spectrophotometer is a precision instrument. Careful assembling and final test have been done
before delivery. Appropriate routine attention and maintenance will not only ensure the reliability and stability of
the instrument but also extend its operating lifetime.
8.1 Points for Attention

• A good environmental condition should be provided according to the requirements in Section 1.4.
• Since the instrument has been adjusted to its optimum conditions before delivery, users are neither allowed to
adjust it by themselves nor dismount parts from the instrument. Pay special attention not to touch and damage
the surfaces of optics or to wipe the surfaces carelessly.
8.2 Daily Care

• Check if there is spilt sample solution contained in the sample compartment after each measurement. The
sample compartment should be cleaned frequently to avoid corrosions of the parts and optical the system.
• The instrument should be covered with anti-dust cover, which is provided with the instrument. Silica-gel bags
can be put in the sample compartment and the light source housing to keep dry. But be sure to take the bags out
before switching on the instrument.
• During daily operation and storage, the LCD display and keyboard should be handled with care and prevented
from scratch, water, dust and corrosion.
• Periodically check the performance of the instrument. If anything abnormal occurs, contact the seller or the
manufacturer immediately.
• If the instrument is not to be used for a long time, pay great attention to environmental conditions such as
temperature and humidity and periodically replace the silica-gel bags.
8.3 Trouble Shooting and Maintenance

8.3.1 Diagnoses of initialization
When the power switch is turned on, the spectrophotometer makes various checks and sets many initialized
parameter values (as shown in Fig. (8-1)). The system gives status information after each operation is completed. If
no abnormality is detected, it displays “OK”. If an abnormality is detected, it displays “Err” and asks whether the
initialization continues or not. The status of instrument will be known after finishing the initialization.
Fig.(8-1) Initialization page

8.3.2 Trouble shooting
If the spectrophotometer malfunctions, it probably is due to the deterioration of consumable parts, operator
mis-operation or incomplete maintenance. Make checks according to the following table. If a major difficulty
38

occurs, contact our service personnel.

Table 8-1
Phenomena Causes Diagnoses Remedy
The instrument does not A) Power cable is not A) Check the power cable; A) Connect it securely;
operate at all, after the securely connected; B) Check the fuse; B) Replace the fuse (2A);
power is turned on. B) Fuse is blown; C) Check the instrument. C) Contact our service
C) Circuit failure. personnel.
Spectrum can not be A) Printer failure; A) Check printer; Contact our service
printed out. B) CPU failure. B) Check CPU board. personnel.
Initialization abnormality:
1) ROM check A) ROM failure; A) Switch on power again to A) Contact our service
check; personnel;
2)Wavelength origin; A) Abnormality of WL A) Switch on power again to A) Contact our service
origin ; check; personnel;
3) Multi-cell origin; A) Abnormality of A) Shift cell holder to check; A) Remove the obstacle;
multi-cell holder drive B) Loose mechanical B) Tighten the connecting
origin; connection; mechanism;
4) Slit origin; A) Slit mechanism A) Switch on power again to A) Contact our service
failure check; personnel;
5) Filter origin; A) Filter mechanism A) Switch on power again to A) Contact our service
failure; check; personnel;
6) W lamp energy; A) Opaque object in the A) Open the sample A) Remove the opaque
sample compartment; compartment; object;
B) W lamp is not lit B) Open the light source B) Replace the W lamp ;
housing;
7) D2 lamp energy; A) Opaque object in the A) Open the sample A) Remove the opaque
B) D2 lamp is not lit; B) Open the light source B) Replace the D2 lamp;
housing;
C) Remove the cover of C) Replace the fuse of D2
instrument; lamp;
8) Wavelength check A) Opaque object in the A) Open the sample A) Remove the opaque
B) Wrong positioning of B) Open the light source B) Reposition the D2
D2 lamp. housing. lamp.
Noise abnormality A) Light source ageing; A) Measure in Energy mode; A) Replace the light
B) Wrong position of the B) Observe at 550nm; source;
sample compartment; C) Check voltage/source of B) Reposition the sample
C) Low voltage / strong magnetic field;
compartment;
magnetic field; D) Measure in Energy mode;
D) Receiver ageing ; E) Check the preamplifier C) Add voltage stabilizer
E) Preamplifier board board; /eliminate interference;
failure; F) Check the drive board. D) Contact our service
F) Abnormality of AD personnel;
converter. E) Contact our service
personnel;
F) Contact our service
personnel.
8.4 Light Source Replacement
8.4.1 Replacement of the W lamp
1. The tungsten lamp is plugged in a socket and sleeved by a plastic holder. It is well adjusted before delivery.
Directly insert a new one when lamp replacement is made.
2. Open the light source housing cover. Loosen the two holding screws (1) on the W lamp socket and remove the
W lamp holder (W) (refers to Fig. 8-2).
3. Replace a new W lamp holder. Clean the surface of the W lamp with a clean cloth socked with alcohol.
39

4. Insert the W lamp holder into the W lamp socket in the light source housing and tighten the two holding
screws (1).
5. If the performance of the instrument changes greatly after the replacement, the height of W lamp should be
adjusted (The horizontal position of W lamp is adjusted automatically when the self-testing is done upon
switching on the power.). The adjusting procedure is as follows:
a) Remove the W lamp holder.
b) Loosen the three screws (2) on the W lamp holder with a watch screwdriver.
c) Adjust the height of W lamp.
d) Tighten the three screws (2) .
e) Insert the W lamp holder back to the socket.
8.4.2 Replacement of the D2 lamp

1. The D2 lamp is plugged in the D2 lamp socket. When replacement is made, directly and securely insert a new
one into the socket.
2. Open the light source housing cover. Remove the D2 lamp (D) (refers to Fig. 7-2) .
3. Replace a new D2 lamp. Clean the surface of D2 lamp with a clean cloth socked with alcohol.
4. Insert the D2 lamp into its socket. [Note: The light beam window should face to the reflective mirror (G)]
PRECAUTIONS:
• The light source and light source housing are considerably heated
during operation. Therefore, be sure to turn off the power switch so as
to cool down the light source sufficiently before replacing the light
source.
• The reflective mirror (G) should be covered with paper bag to avoid
being contaminated by fingers.
Fig. (8-2) Diagram of light source housing
40

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INSTRUCTION MANUAL

Chapter 2 Instrument Specifications……………………………………………………………………………6

Chapter 3 Photometric Measurement……………………………………………………………………………7

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Chapter 4 Spectrum Scanning………………………………………………………………………………15

Chapter 5 Quantitative Measurement ………………….………………………………………………………21

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Chapter 6 DNA/Protein Analysis………………………………………………………………………………31

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1.1 Inspection of Parts

Table 1-1 Standard Contents

1.2 General Description of the Spectrophotometer

1.2.2 Back side of the instrument

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(1) Function keys (F1~F5)

(3) Edit keys ( ▲ , ▼ , , , , RETURN)

data while setting time.

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(4) Operation keys (AUTOZERO, GOλ , START/STOP)

Wavelength set key [GOλ ]: Used to set the current wavelength.

Start/Stop key [START/STOP]: Used to start or stop a measurement.

a. Ambient temperature: 15℃~35℃

b. Relative humidity: less than 80%

1.5 Turning on the Instrument

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1.6 Performance Check at Installation

1.6.1 Wavelength accuracy and reproducibility

1.6.2 Baseline flatness

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Model SpectroScan 80D SpectroScan 80DV

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3.1 Information Display and Function Keys

Fig.(3-1) The working picture of photometric measurement

3.2 Parameter Setting

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Fig. (3-2) Parameter setting page of photometric measurement

3.2.1 Setting photometric mode

Fig.(3-3) Mode setting page in photometric measurement

Fig. (3-4) Wavelength setting page in photometric measurement

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3.2.3 Setting K-Factor

Article I. Fig. (3-5) K-Factor setting page in

3.2.4 Quitting parameter setting

3.3 Setting Working Wavelength

set the working wavelength for photometric measurement.

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Fig. (3-6) Wavelength setting page in photometric measurement

3.4 Auto-zeroing (100%T setting)

Fig. (3-7) Sample control page in photometric measurement

3.5.1 Selecting sample module type

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3.5.2 Setting sample cell number in use (Drive Cell No.)

Fig. (3-8) Sample cell number setting page

3.5.4 Sample cell shifting (Move Cell)

3.5.5 Resetting to cell No.1(Move Home)

3.5.6 Single-cell measurement