St.

Bridget College
Batangas City, Philippines
COLLEGE DEPARTMENT
PAASCU Accredited
Contact No. (043) 723-3616

Name: Anjaneth G De Austria Date:

September 22,2021
Course:Bsed Sci
Laboratory Experiment 1
Onion Cell Microscope Slide Experiment

Introduction:
Many things that are viewed using microscope particularly cell can
appear quite transparent under the microscope. The internal parts of the
cell, the organelles, are so transparent that they are often difficult to
see. Biologist have developed a number of stains help them to see the
cells and their organelles. In this experiment we will try to see what is
the structure of cell using an onion cell in a microscope.
Objective:
 Identify the structure that is visible in an onion cell and it's shape
 To know why onion is an easy experiment materis/tools


Materials:
 Microscope
 Microscope Camera
 Microscope Slide
 Knife
 Toothpicks
 Pipettes/ Dropper
 Cover slips
 Onion
 Iodine Tincture
 Cutting board

Procedures:
1. Preparing the slides
2. Wash and peel the onion
3. Slice the onion as thin as possible
(Thin layer of skin around the onion work best)
4. Clean your microscope slide with an alcohol wipe
(Do the same with cover slip)
M. H. Del Pilar St., Batangas City, 4200 Philipines
www.sbcbatangas.edu.ph
 St. Bridget College
Batangas City, Philippines
COLLEGE DEPARTMENT
PAASCU Accredited
Contact No. (043) 723-3616

5. Place your pieces of onion onto the slide and reposition them with
toothpick
6. A drop of Iodine onto the onion
7. Drop on the cover slip( apply pressure to coverslip but not enough to
break it.
8. Now your slide is complete
9. Observe under microscope

Analysis:
1. What is your observation ? Can you paste or draw the result of the
experiment.
-I observe that there are large, rectangular interlocking cells,
Clearly visible distinct cell walls surrounding the cells,Dark
stained nucleus, Large vacuoles at the center and Small granules
may be observed inside the cells (within the cytoplasm)

2 .What structure are visible in an onion cell?

- Onion Cell
The vacuole is prominent and present at the centre of the cell. It is
surrounded by cytoplasm. The presence of a cell wall and a large
M. H. Del Pilar St., Batangas City, 4200 Philipines
www.sbcbatangas.edu.ph
 St. Bridget College
Batangas City, Philippines
COLLEGE DEPARTMENT
PAASCU Accredited
Contact No. (043) 723-3616

vacuole are indicators that help identify plant cells, such as seen
in the onion peel.
2. What are the shape of an onion cell?
- The shape of an onion cell is square or rectangular. The interior
of the cell is easy to see and focuses well for microscopic
observation.
3. In your opinion, why onion is used in an experiment ?
-An onion is made up of layers that are separated by a thin
membrane. For this experiment, the thin membrane will be used to
observe the onion cells. It can easily be obtained by peeling it from
any layer of the onion using tweezers.
4. Why they use iodine tincture for onion cell?
-The onion rigid cell walls allows cells to viewed in the microscope
and the iodine dye is a red allowed it to be seen.

Conclusion:

I conclude that the bulb of an onion is formed from modified

leaves. While photosynthesis takes place in the leaves of an onion
containing chloroplast, the little glucose that is produced from this
process is converted in to starch (starch granules) and stored in
the bulb. Chlorophil and chloroplasts responsible for
photosynthesis are therefore only present in the leafy part of the
onion (above ground) and absent in the bulb (which grows below
ground). Unlike animal cells (such as cheek cells) the cell wall of
an onion and other plants are made up of cellulose, which protects
the cell and maintains its shape.

M. H. Del Pilar St., Batangas City, 4200 Philipines

www.sbcbatangas.edu.ph
 St. Bridget College
Batangas City, Philippines
COLLEGE DEPARTMENT
PAASCU Accredited
Contact No. (043) 723-3616

Learning Activity 1
Cell Analogy
Direction: Choose the word best to complete the analogy.
Example:(Golgi Apparatus:Post Office)(Explanation:Golgi apparatus is
sorted the packed and distributed around the cell just like post office
would do with mail)

1. Nucleus: J A. City Wall

2. Ribosomes: G B. Warehouse
3.Lysosomes: I C. Police Officer
4. Vacuoles: B D. Office in the City Hall
5. Cell membrane: A E. Solar Energy Plants
6 Mitochondria: H F. Garbage Disposal
7 Cell wall: C G. Food producing Factory
8Nucleolus: D H.Power Plant
9 Smooth Endoplasmic I. Manufacturing Plant
Reticulum: f
10 Chloroplast: E J. City Hall

M. H. Del Pilar St., Batangas City, 4200 Philipines

www.sbcbatangas.edu.ph