Product datasheet

Universal Lateral Flow Assay Kit ab270537

1 References 2 Images

Overview

Product name Universal Lateral Flow Assay Kit

Product overview
Universal Lateral Flow Assay Kit (ab270537) is designed to enable simple and rapid
development of proof-of-concept sandwich lateral flow immunoassays. The kit includes everything
needed for lateral flow assay development with any pair of capture and detection antibodies. It
combines universal LFA strips, Lightning-Link® and Gold conjugation technologies, buffers and
controls, which means there is no need to strip the capture antibody onto LFA strips using
expensive equipment and complex procedures or conjugate the detection antibody to gold with
passive absorption. The signal intensities can be qualitatively analyzed using the supplied scoring
card or. For a quantitative detection, an LFA reader can be used.

This kit contains Gold conjugation kit (ab154873)

Benefits:

Adaptable to any pair of capture and detection antibodies

Quick and easy to use – conjugation protocols require only 30 seconds hands-on time

No need to strip or print capture antibodies

No passive absorption of detection antibodies

No specialised or costly equipment required

Results can be qualitatively and quantitatively analysed using the scoring card, or an LFA reader,
respectively

This product is manufactured by Expedeon, an Abcam company. It was previously called

Universal LFA Kit. Expedeon product code 4300-0100 is the same as the 100-test size of this
product.

Tested applications Suitable for: LFA

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Properties

Storage instructions Please refer to protocols.

Components 100 tests

ab274297 - 40nm GOLD-Biotin, 10 OD 1 x 200µl

ab274107 - 96-well clear low binding plates 2 units

ab154873 - Gold Conjugation Kit (40nm, 20 OD) 1 kit

ab274106 - Modifier reagent 1 x 200µl

ab274296 - Quencher reagent 1 x 200µl

Scoring card 1 unit

ab274105 - Ulfa-Tag mix 3 x 100µg

ab274103 - Universal LFA strips 1 x 100 tests

ab274104 - Universal Running Buffer 2 x 1500µl

Applications

The Abpromise guarantee Our Abpromise guarantee covers the use of ab270537 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes

LFA Use at an assay dependent concentration.

Images

The capture antibody is conjugated to Lightning-Link® Ulfa-Tag,

while the detection antibody is conjugated to 40 nm Gold, both of
which require only 30 seconds to set up. The capture and detection
antibody are diluted and incubated with the analyte and 40 nm Gold-
Biotin and then run on Universal LFA strips. Universal LFA strips
consist of a nitrocellulose membrane containing a ‘Test line’ (T-line)
of immobilized anti-Ulfa-Tag antibody, that binds the Ulfa-Tag
conjugated capture antibody which further binds the analyte in
complex with the Gold-detection antibody. A red T-line appears
when the analyte is present, and the line intensity varies depending
on the analyte concentration. Universal LFA strips also contain a
How this kit works
‘Control-line’ (C-line) that exploit the extraordinarily high affinity of

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 streptavidin for biotin, which shows that the test is valid, and an
absorbent pad to promote and control the flow of sample through
the membrane.

Example of sandwich LFA assay for the detection of CRP in serum

using the Universal Lateral Flow Assay kit: Ulfa-Tag and 40nm
GOLD are used to conjugate the capture and detection antibody
respectively. Results were analyzed using a Qiagen ESEQuant
reader.

Example data

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"

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Replacement or refund for products not performing as stated on the datasheet

Valid for 12 months from date of delivery
Response to your inquiry within 24 hours

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If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit https://www.abcam.com/abpromise or contact our technical team.

Terms and conditions

Guarantee only valid for products bought direct from Abcam or one of our authorized distributors