Name: ________________________________ Date: ___________

Section: _______________________________ Score: __________

EXERCISE NO. 3
Volumetric Measurements

INTRODUCTION
Volumetric Measurement plays a central role in the laboratory. The user has to
determine the degree of accuracy required for each measurement. Based on this, we
can choose the appropriate volumetric instrument.
 
OBJECTIVES:
     At the end of this exercise, the students should be able to:
1. Determine the accuracy of various types of graduated glasswares.
2. Measure volumes of liquids using graduated glasswares.
3. Recognize different equipment in dosage measurements, both for oral and parenteral
administration.
4. Distinguish the different types of syringes and their specific measurements
 
MATERIALS:

 Device (phone, tablet or laptop)

  Website/Search engines
 Syringes (3 cc syringe, 5 cc syringe, Tuberculin syringe, Insulin syringe, Pre-filled
or Single-Dose syringe)

I. CALIBRATION OF GLASSWARES

A. Pipette and Beaker (Please watch the attached video and record your observations.)

Procedure:

1. Rinse, clean and dry 10mL pipette, and 250 mL beaker.

2. Fill the pipette with distilled water using an aspirator.

3. Transfer the water from the pipette into the beaker.

4. Perform step 2 and 3 for five times.

5. Record the volume of water transferred in the beaker.

Results:

Volume of distilled water in the pipette

1st transfer
2nd transfer 
3rd transfer
4th transfer
5th transfer
Total volume of water transferred
Actual volume of water in the beaker

Question to Answer:

Do the pipette and beaker have similar calibrations? If yes, explain why. If not, which do you
think of these two glasswares is more accurate? Explain.
______________________________________________________________________________
______________________________________________________________________________
______________________________________________________________________________
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B. Graduated cylinder (Please watch the attached video and record your observations.)

Procedure:

1. Rinse, clean and dry 100mL graduated cylinder.

2. Fill the graduated cylinder with one bottle of Yakult. Record the volume. Repeat the
procedure for the remaining bottles.

3. Compute for the mean and standard deviation of the results.

4. Compare the results with the volume labeled on the bottle.

Record your observations.

Results:

Actual volume in the

Volume labeled on the bottle
graduated cylinder
1st bottle
2nd bottle
3rd bottle
4th bottle
5th bottle
MEAN
STANDARD DEVIATION

 
II. EQUIPMENT USED IN DOSAGE MEASUREMENT

Identify and describe the following equipment used in dosage measurement, and indicate their
uses.

A. Oral administration

Figure 3.a

NAME OF EQUIPMENT: Medicine cup

DESCRIPTION: They are little cups, such as the Premier Plastic 1 oz cup, that carry just 1 to 2
fluid ounces. They're made of plastic or paper and are meant to be thrown away. Dry medicines
are frequently administered in paper medicine cups.
USE OR FUNCTION: Medicine Cups are portion, soufflé, and measurement cups used to
dispense medicines.

Figure 3.b
NAME OF EQUIPMENT: Calibrated medicine dropper
DESCRIPTION: 1/4 ML increments were used to calibrate the device.
Rubberized surface. Capacity: 1 ML Glass allows for easy viewing. The dropper shaft is
transparent, while the bulb and graduations are black.
USE OR FUNCTION: Calibrated medicine droppers are frequently used to provide tiny dosages
of liquid medicines to patients (1 mL or less). Dropper dosage precision and accuracy might be
quite low.

Figure 3.c

NAME OF EQUIPMENT: Eccentric tip syringe

DESCRIPTION: Eccentric tip syringes have a nozzle that is offset from the syringe barrel's
edge. This brings the connected needle closer to the syringe walls, allowing it to be almost
parallel with the injection surface.
USE OR FUNCTION: Eccentric tip syringes have an off-center tip and are frequently used for
injections into surface veins or arteries. A needle is usually included with insulin syringes. To
assist prevent discomfort and bruising, they use a short, thin needle. An insulin syringe's barrel
is long, thin, and labelled in units of insulin.
 Figure 3.d

NAME OF SYRINGE: 3 ml syringe

USE OR FUNCTION: diabetic and other medical applications, such as post-operative situations,
vitamin shortages, and intramuscular medicine This 3 mL Syringe is single-use and sterile. 3 mL
Syringes are packaged in a blister packaging that is easy to peel.

Figure 3.e

NAME OF SYRINGE: 5 ml syringe

USE OR FUNCTION: Injections for medicinal purposes. 5 mL Syringes are used to properly
measure liquid medication dosages up to 5 cc. These Syringes with Needle, manufactured by
Becton Dickinson, come with a transparent barrel to enable for more precise dose measurement.
 Figure 3.f

NAME OF SYRINGE: Tuberculin syringe

USE OR FUNCTION: are needle-based devices that measure and distribute a precise volume of
liquid. Medicines, vaccines, and other chemicals are injected subcutaneously or intradermally.

Figure 3.g

NAME OF SYRINGE: Insulin Syringes

USE OR FUNCTION: Insulin is delivered to diabetic individuals using this device. The needle,
barrel, and plunger are the three components of an insulin syringe. The size of an insulin syringe
ranges from 0.25 mL to 1.0 mL. The amount of units a syringe can carry is indicated by its size.
As a result, a 0.40 ml of insulin may store 40 units.

 
 Figure 3.h

NAME OF SYRINGE: Lidocaine Hydrochloride Injection

USE OR FUNCTION: For nerve inhibition, the injectable form provides a wide variety of uses.
Percutaneous infiltration, blocking a major nerve plexus such as the brachial, epidural
anaesthesia, and intravenous regional analgesia are all options.

 
Label the parts of a syringe and indicate its function.

Figure 3.i

No. Name of Part Function

The hub is the portion of the needle that
connects to the syringe at one end. The shaft is
No. 1 Hub the needle's long, slender stem, which is
beveled on one end to produce a tip. The lumen
refers to the hollow bore of the needle shaft.
The liquid to be dispensed is stored in a
cylinder. The volume of the barrel below the
No. 2 Barrel
seal is reduced when pressure on the seal expels
fluid.
The shaft that joins the Plunger Flange with the
seal. The pressure exerted on the seal by this
No. 3 Plunger
shaft as it presses against the liquid in the
barrel.

Questions to Answer:

1. Give an example of glassware that is used for accurate measurement of volume.

 Graduated cylinders, beakers, volumetric pipets, burets, and volumetric flasks are five
different types of glassware that are commonly used to measure particular quantities. The most
precise are volumetric pipets, flasks, and burets, which are calibrated to a high level of precision
by glassware manufacturers.

2. Can 1.25mL be measured in the regular 3mL syringe? If yes, How?

 Yes, Measure as 1.3 mL on the mL scale after rounding to 1.3.

3. Should insulin be measured in a 1mL syringe? Explain your answer.

 Insulin is measured in International Units (units); most insulin is U-100, which implies
that 100 units of insulin equal one milliliter.

4. What are the primary purpose of safety and needleless syringes?

 A safety syringe is a syringe that has a built-in safety mechanism to decrease the danger
of needlestick injuries to healthcare professionals and others. The needle on a safety syringe
might be removable or permanently connected. On certain versions, a sheath is put over the
needle, but on others, the needle retracts into the barrel.

Reference/s:

Pradyot Patnaik (2003). "Specifications for volumetric ware". Dean's Handbook of Analytical
Chemistry, 2nd Edition. McGraw-Hill

Sinding C (2010-12-08). "Making the unit of insulin: standards, clinical work, and industry,
1920–1925". Bull Hist Med.
"WHO | Safe syringes for injection safety". World Health Organization. 2010-11-30. Retrieved
2012-02-07.

Bethany, Asked by. "Is it safe to reuse an insulin syringe? - CNN.com". Archived from the
original on 2017-07-10.

"BD Diabetes-Syringe Capacity and Dose Size". Bd.com. Archived from the original on 2011-07-
07. Retrieved 2010-12-30.