(from 1-186 bp) 18/11/2021 22:40:48

2-F-DOC (400 bp)

BsoBI
AvaI AflII
MnlI BsaJI PsiI SmlI PsiI NlaIV
GAAACCCCGAGGGGAACTATATCAGGGTCAGGCACTTTATAACCATCAATAGTCCATCTTAAGTTATAAACAAAAGTAGTTAAAATGGTTCCC
CTTTGGGGCTCCCCTTGATATAGTCCCAGTCCGTGAAATATTGGTAGTTATCAGGTAGAATTCAATATTTGTTTTCATCAATTTTACCAAGGG
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30
N P E G N Y I R V R H F I T I N S P S * V I N K S S * N G S
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30
K P R G E L Y Q G Q A L Y N H Q * S I L S Y K Q K * L K W F P
132 130 128 126 124 122 120 118 116 114 112 110 108 106 104
V G L P V I D P D P V K Y G D I T W R L N Y V F T T L I T G
132 130 128 126 124 122 120 118 116 114 112 110 108 106 104
F G R P S S Y * P * A S * L W * Y D M K L * L C F Y N F H N G
132 130 128 126 124 122 120 118 116 114 112 110 108 106 104
F G S P F * I L T L C K I V M L L G D * T I F L L L * F P E W
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30
E T P R G T I S G S G T L * P S I V H L K L * T K V V K M V P

10 20 30 40 50 60 70 80 90

Hpy166II PflMI
MfeI AccI BslI HphI
AATTGAACATAAGCAAATTGTTCCCCAATACATCTATGTCTACCACCACCAAATGGTAAATAAGGTGAAGAAACCCCTTTAGAAACTTTCCCA
TTAACTTGTATTCGTTTAACAAGGGGTTATGTAGATACAGATGGTGGTGGTTTACCATTTATTCCACTTCTTTGGGGAAATCTTTGAAAGGGT
32 34 36 38 40 42 44 46 48 50 52 54 56 58 60
Q L N I S K L F P N T S M S T T T K W * I R * R N P F R N F P
32 34 36 38 40 42 44 46 48 50 52 54 56 58 60 62
I E H K Q I V P Q Y I Y V Y H H Q M V N K V K K P L * K L S Q
102 100 98 96 94 92 90 88 86 84 82 80 78 76 74 72
L Q V Y A F Q E G I C R H R G G G F P L Y P S S V G K S V K G
102 100 98 96 94 92 90 88 86 84 82 80 78 76 74 72
I S C L C I T G W Y M * T * W W W I T F L T F F G R * F S E W
102 100 98 96 94 92 90 88 86 84 82 80 78 76 74 72
N F M L L N N G L V D I D V V V L H Y I L H L F G K L F K G L
32 34 36 38 40 42 44 46 48 50 52 54 56 58 60 62
N * T * A N C S P I H L C L P P P N G K * G E E T P L E T F P

100 110 120 130 140 150 160 170 180

https://benchling.com/dannily/f/lib_TUMW30LU-erg-11/seq_RLBR8gCG-2-f-doc/edit 1/3
 2-F-DOC (400 bp) (from 187-372 bp) 18/11/2021 22:40:48

Fnu4HI
ApeKI BbvI
Fnu4HI BtsIMutI DpnI
ApeKI TspRI Sau3AI
TseI TscAI DpnII EcoP15I
BsmFI TseI BtsαI BstXI AlwI
Hpy188I CviKI-1 MmeI BtsI AcuI BfuCI EcoP15I
EarI AluI MwoI BbvI BfaI MboI BsaBI
AACCCATAATCAACTTCATCAGAAGAGTTAAATGAAACAGAATTAGCTTTGGCAGCAGCAGTGTCCCATCTAGTTGGATCAAAATCTTCAGGG
TTGGGTATTAGTTGAAGTAGTCTTCTCAATTTACTTTGTCTTAATCGAAACCGTCGTCGTCACAGGGTAGATCAACCTAGTTTTAGAAGTCCC
62 64 66 68 70 72 74 76 78 80 82 84 86 88 90 92
K P I I N F I R R V K * N R I S F G S S S V P S S W I K I F R
64 66 68 70 72 74 76 78 80 82 84 86 88 90 92
T H N Q L H Q K S * M K Q N * L W Q Q Q C P I * L D Q N L Q G
70 68 66 64 62 60 58 56 54 52 50 48 46 44 42
F G Y D V E D S S N F S V S N A K A A A T D W R T P D F D E P
70 68 66 64 62 60 58 56 54 52 50 48 46 44 42
V W L * S * * F L * I F C F * S Q C C C H G M * N S * F R * P
70 68 66 64 62 60 58 56 54 52 50 48 46 44 42
G M I L K M L L T L H F L I L K P L L L T G D L Q I L I K L T
64 66 68 70 72 74 76 78 80 82 84 86 88 90 92
N P * S T S S E E L N E T E L A L A A A V S H L V G S K S S G

190 200 210 220 230 240 250 260 270

ScrFI
BstNI
MslI StyD4I
BfaI PspGI BpmI HinfI
SpeI BssKI HpyCH4IV TfiI
TTATCAAAATATCTTTCACTAGTATGAGCATAACCTGGAGAAACTAAAACGTAATGACCTTTTGGAACAATATAATTGGGTTCAGGGATTCTT
AATAGTTTTATAGAAAGTGATCATACTCGTATTGGACCTCTTTGATTTTGCATTACTGGAAAACCTTGTTATATTAACCCAAGTCCCTAAGAA
94 96 98 100 102 104 106 108 110 112 114 116 118 120 122
V I K I S F T S M S I T W R N * N V M T F W N N I I G F R D S
94 96 98 100 102 104 106 108 110 112 114 116 118 120 122 124
Y Q N I F H * Y E H N L E K L K R N D L L E Q Y N W V Q G F L
40 38 36 34 32 30 28 26 24 22 20 18 16 14 12 10
N D F Y R E S T H A Y G P S V L V Y H G K P V I Y N P E P I R
40 38 36 34 32 30 28 26 24 22 20 18 16 14 12 10
* * F I K * * Y S C L R S F S F R L S R K S C Y L Q T * P N K
40 38 36 34 32 30 28 26 24 22 20 18 16 14 12 10
I L I D K V L I L M V Q L F * F T I V K Q F L I I P N L S E *
94 96 98 100 102 104 106 108 110 112 114 116 118 120 122 124
L S K Y L S L V * A * P G E T K T * * P F G T I * L G S G I L

280 290 300 310 320 330 340 350 360 370

https://benchling.com/dannily/f/lib_TUMW30LU-erg-11/seq_RLBR8gCG-2-f-doc/edit 2/3
 2-F-DOC (400 bp) (from 373-400 bp) 18/11/2021 22:40:48

NlaIV
Sau96I
AvaII BsmFI
AATGGGACCAGCAGAAATCATTTGGGGG
TTACCCTGGTCGTCTTTAGTAAACCCCC
124 126 128 130 132
* W D Q Q K S F G
126 128 130 132
M G P A E I I W G
8 6 4 2
L P V L L F * K P
8 6 4 2
I P G A S I M Q P
8 6 4 2
H S W C F D N P P
126 128 130 132
N G T S R N H L G

380 390 400

https://benchling.com/dannily/f/lib_TUMW30LU-erg-11/seq_RLBR8gCG-2-f-doc/edit 3/3