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Chat Zi Vasile I Ou 2017
Chat Zi Vasile I Ou 2017
RESEARCH
Effectiveness of Denture Cleansers on Removal
of Adherent Candida albicans Cells from
Denture Base Acrylics of Various Roughness
Konstantinos Chatzivasileiou, DDS, MSc
Eleni Kotsiomiti, DDS, PhD
Department of Prosthodontics, School of Dentistry, Faculty of Health Sciences,
Aristotle University of Thessaloniki, Thessalokini, Greece.
Purpose: To experimentally examine the effectiveness of a small sample of commercial denture cleansers
in removing Candida albicans cells from denture surfaces. Materials and Methods: A total of 216
specimens from three brands of denture base resins (72 for each acrylic resin) were divided into three
groups of 24 specimens that each received a different surface treatment (Ra1, Ra2, and Ra3). The specimens
were contaminated by the Candida albicans strain ATCC 90028, immersed for 15 minutes in one of two
experimental denture cleanser solutions or in tap water, and placed in Petri dishes with culture medium.
Results: Candida albicans colonies were measured after 24-hour incubation at 37°C. There was a statistically
significant difference in the cleansing result depending on the denture cleanser used. Conclusion: The use
of commercial denture cleansers may under certain conditions be effective in the removal of Candida
albicans from denture bases. Int J Prosthodont 2019;32:196–197. doi: 10.11607/ijp.6041
R
emovable prostheses function in an environment colonized by numerous mi-
croorganisms able to attach to hard, nonshedding surfaces, which can lead to
plaque formation and retention.1,2 Plaque retention is associated with various
diseases. Denture-related stomatitis is the most easily recognized of these diseases
and involves Candida species in about 90% of cases.3
The purpose of this study was to experimentally examine the effectiveness of a small
sample of commercial denture cleansers in removing Candida albicans (C albicans)
cells from acrylic resin denture base surfaces.
A total of 216 specimens from three brands of denture base resins (Paladon 65
[Heraeus], ProBase Hot [Ivoclar Vivadent], and Lucitone 199 [Dentsply]), 72 specimens
for each resin, were divided into three groups of 24 specimens that each received a
different surface treatment (surface roughness [Ra] groups; Ra1: laboratory polishing;
Ra2: tungsten carbide bur; or Ra3: silicone polishers) and were sterilized by exposure
to ultraviolet (UV) light.
The specimens were contaminated by adding 20 μL of 0.5 McFarland C albicans
suspension (ATCC 90028) to 20 mL of Sabouraud dextrose broth + chloramphenicol
Correspondence to:
Dr Konstantinos Chatzivasileiou 0.05% for 24 hours at 37°C.
19is Maiou 69 The specimens in each of the three groups (Ra1, Ra2, Ra3) were divided into four
Drama, 66132, Greece subgroups (A, B, C, D) of six specimens each. Each specimen in subgroup A was
Fax: +302521030587
Email: khatziv@gmail.com placed in a Petri dish containing culture medium (Sabouraud dextrose agar + chlor-
amphenicol 0.05%) for 10 minutes and then discarded. For subgroups B, C, and D,
Submitted July 3, 2018; each specimen was placed in a sterile container with one of the following liquids:
accepted July 26, 2018.
©2019 by Quintessence 250 mL of warm tap water (35°C) (subgroup B); 250 mL of warm tap water (35°C)
Publishing Co Inc. and a Corega denture cleansing tablet (GlaxoSmithKline) (subgroup C); and 250 mL
© 2019 BY QUINTESSENCE PUBLISHING CO, INC. PRINTING OF THIS DOCUMENT IS RESTRICTED TO PERSONAL USE ONLY.
NO PART MAY BE REPRODUCED OR TRANSMITTED IN ANY FORM WITHOUT WRITTEN PERMISSION FROM THE PUBLISHER.
Chatzivasileiou et al
of warm tap water (35°C) and a Protefix denture cleansing Table 1 Effect of Denture Cleanser on
tablet (Queisser Pharma) (subgroup D). Each specimen was Candida albicans Adherence in Relation to
kept immersed for 15 minutes and was then placed in a Polishing Method and Acrylic Resin Brand
Petri dish, as in subgroup A. Acrylic brand Polishing method P
Paladon 65 Laboratory .000
Plates were incubated at 37°C for 24 hours, after which Bur .000
C albicans colonies were measured in colony-forming units EVE .000
(CFUs). Data were statistically analyzed using analysis of ProBase HOT Laboratory .000
variance (ANOVA). Effects were considered significant at Bur .000
EVE .000
P < .05. Lucitone 199 Laboratory .000
Bur .000
RESULTS EVE .000
Only statistically significant differences (P < .05) are shown.
© 2019 BY QUINTESSENCE PUBLISHING CO, INC. PRINTING OF THIS DOCUMENT IS RESTRICTED TO PERSONAL USE ONLY.
NO PART MAY BE REPRODUCED OR TRANSMITTED IN ANY FORM WITHOUT WRITTEN PERMISSION FROM THE PUBLISHER.