ASAM GLUTAMAT DITITRASI DENGAN HCl

Volume HCl
pH
(mL)

0 5.4 DAPAT DITENTUKAN NILAI PKA DAN NILAI ISOELEKTRIKNYA

1 3.96
2 2.67
Kurva Titrasi Asam Glutamat dengan HCl
3 2.49
6
4 2.29
5 2.13 5 f(x) = − 1.365 x + 5.375
R² = 0.998994691973621
6 2.01
4
7 1.85
8 1.72 3

pH
9 1.64 2 f(x) = − 0.116318681318681 x + 2.75208791208791
R² = 0.956829314741403
10 1.48
1
11 1.41
12 1.36 0
0 2 4 6 8 10 12 1
13 1.33
Volume HCl (mL)
14 1.3

Volume NaOH
pH
(mL)
0 5.84
1 9.37
Kurva Titrasi Asam Glutamat dengan NaOH
2 10.27
14
3 10.83
4 11.14 12 f(x) = 0.053064425770308 x + 10.8471260504202
R² = 0.718806756919959
5 11.26 10
6 11.34 8
f(x) = 3.53 x + 5.84
R² = 1
7 11.48
pH

6
8 11.5
4
9 11.6
10 11.6 2
11 11.69 0
0 5 10 15 20 25 30 35
12 11.73
Volume NaOH (mL)
13 11.79
14 11.85
15 11.88
16 11.91
17 11.94
18 11.97
19 11.99
20 12.01
21 12.05
22 12.13
23 12.15
24 12.25
25 12.15
26 12.15
27 12.17
28 12.19
29 12.37
30 12.38
31 12.38
32 12.39
33 12.39
34 12.39
35 12.39
 Volume HCl
pH
(mL) Kurva
DAN NILAI ISOELEKTRIKNYA 0 7.69 9
1 5.12 8

2 3.41 7 f(x) = − 2.14 x + 7.54666666

m Glutamat dengan HCl 6
R² = 0.986720483779455
3 3.1
5
4 2.84

pH
4
5 2.5
3 f(x) = − 0.2
6 1.92 R² = 0.9379
2
7 1.72 1
8 1.68 0
9 1.47 0 2
1318681 x + 2.75208791208791
1403
10 1.34
11 1.3
8 10 12 14 16
Volume HCl (mL)

Volume
pH
NaOH (mL)
0 7.42 Kurva
1 10.81 14
m Glutamat dengan NaOH
2 11.06 12 f(x) = 0.06269924812
3 11.66 R² = 0.690201496803
10 f(x) = 3.39 x + 7.42
+ 10.8471260504202 4 11.84 R² = 1
8
5 11.95
pH

6 12.04 6
7 12.1 4
8 12.16 2
9 12.21
0
10 12.24 0 5
11 12.26
20 25 30 35 40
12 12.3
olume NaOH (mL)
13 12.31
14 12.33
15 12.34
16 12.35
17 12.35
18 12.4
19 12.46
20 12.5
 Konsentrasi Absorba
No x.y
(x)/ppm nsi (y)
1. 1000 0.001 1
2. 3000 0.002 6
3. 5000 0.005 25
4. 8000 0.008 64
5. 10000 0.012 120
Σ 0.028 216
 Kurva Titrasi Tirosin dengan HCl
9
8
7 f(x) = − 2.14 x + 7.54666666666667
R² = 0.986720483779455
6
5
pH

4
3 f(x) = − 0.247393939393939 x + 3.73606060606061
2 R² = 0.937912887202948
1
0
0 2 4 6 8 10 12
Volume HCl (mL)

Kurva Titrasi Tirosin dengan NaOH

14

12 f(x) = 0.062699248120301 x + 11.4251578947368

R² = 0.69020149680368
10 f(x) = 3.39 x + 7.42
R² = 1
8
pH

0
0 5 10 15 20 25
Volume NaOH (mL)

Kurva Baku Larutan Standar Kadar Protein Secara Biuret

Absorbansi

0.014

0.012
 Kurva Baku Larutan Standar Kadar Protein Secara Biuret

Absorbansi
0.014

0.012
x 2

f(x) = 1.21804511278196E-06 x − 0.000977443609023

0.01
R² = 0.972036001333383
1000000 0.008
9000000
0.006
25000000
0.004
64000000
100000000 0.002
199000000 0
0 2000 4000 6000 8000 10000 12000
Konsentrasi (ppm)