8

Biotechnology
Quarter 1B:
Biotechniques
Module I:
Laboratory Apparatus/Equipment
and Uses
Week 5
Welcome to the STE Electives-Biotechnology Alternative Delivery Mode (ADM) my dear learner!

This module will serve as your guide in mastering the most essential learning competencies and
be technologically, environmentally, and scientifically literate. The activities prepared will give you
opportunity for independent practice and learning. Furthermore, you will enjoy the fun in
performing the tasks included in this self-learning kit.

Relax and enjoy another dimension of learning.

This module has the following parts and corresponding icons:

This will give you an idea of the skills or competencies

What I Need to Know you are expected to learn in the module.

This part includes an activity that aims to check what

What I Know you already know about the lesson to take. If you get
all the answers correct (100%), you may decide to skip
this module.
This is a brief drill or review to help you link the current
What’s In lesson with the previous one.

In this portion, the new lesson will be introduced to you

What’s New in various ways such as a story, a song, a poem, a
problem opener, an activity or a situation.
This section provides a brief discussion of the lesson.
What is It This aims to help you discover and understand new
concepts and skills.
This comprises activities for independent practice to
What’s More solidify your understanding and skills of the topic. You
may check the answers to the exercises using the
Answer Key at the end of the module.
This includes questions or blank sentence/paragraph to
What I Have Learned be filled in to process what you learned from the lesson.

This section provides an activity which will help you

What I Can Do transfer your new knowledge or skill into real life
situations or concerns.
This is a task which aims to evaluate your level of
Assessment mastery in achieving the learning competency.

In this portion, another activity will be given to you to

Additional Activities enrich your knowledge or skill of the lesson learned.
This also tends retention of learned concepts.

The following are some reminders in using this module:

1. Use the module with care. Do not put unnecessary mark/s on any part of the module. Use
a separate sheet of paper in answering the exercises.
 2. Don’t forget to answer What I Know before moving on to the other activities included in
the module.
3. Read the instruction carefully before doing each task.
4. Observe honesty and integrity in doing the tasks and checking your answers.
5. Finish the task at hand before proceeding to the next.
6. Return this module to your teacher/facilitator once you are through with it.
If you encounter any difficulty in answering the tasks in this module, do not hesitate to consult
your teacher or facilitator. Always bear in mind that you are not alone.

We hope that through this material, you will experience meaningful learning and gain deep
understanding of the relevant competencies. You can do it!

What I Need to Know

The laboratory equipment is a technical field, which offer opportunity in learning science
through hands-on experience. Only with the help of laboratory equipment, experiments,
process controls and quality assurance are made possible. Electronic measuring
instruments or vacuum apparatuses are the most important working materials in the
field of laboratory equipment. Without the help of these important devices, the scientific
field of laboratory equipment could not exist.

In this module, you will be able to familiarized and understand the uses of Laboratory
apparatus and equipment used in Biotechnology and the proper way of proper handling
some laboratory apparatus and equipment.

In this module, it includes:

● Lesson 1 – Laboratory apparatus/ equipment and Uses

After going through this module, you are expected to:

1. Identify, describe and explain the function of the different laboratory apparatus
and equipment used in Biotechnology.

What I Know

Read each sentences carefully. Write the letter of the correct answer on your answer
sheet.

1. It is a heated and pressurized container used to sterilize equipment and kill any
organisms left.
a. Water bath c. incubator
b. Autoclave d. forceps
2. Used to measure exact amounts of small liquids.
a. centrifuge c. micropipette
b. beaker d. test tube
3. It is used to crushed and mixed solids.
a. Beaker
b. Petri dish
c. Mortar and pestle
d. Graduated cylinder
4. An apparatus consisting essentially of a compartment spun at high speeds to help
separate liquids from solids.
a. centrifuge c. micropipette
b. beaker d. test tube
5. A natural gas-powered piece of equipment that is used to heat, sterilization or
combustion.
a. thermometer c. alcohol lamp
b. Bunsen burner d. iron stand with ring
6. Used to protect eyes during laboratory experiments, must be worn at all times.
a. Sun glasses c. lab gown
b. goggles d. gloves
7. A local ventilation unit used to limit exposure to toxic fumes or dusts.
a. Distiller c. fume hood
b. Test tube d. Test Tube
8. If you break a glass container during lab what should you do?
a. Clean it up immediately to avoid accidents
b. Yell “Code One” and let your teacher clean it up.
c. Leave it and tell your teacher after the lab work completed.
d. Alert your lab partners and other groups near you and tell your teacher.
9. Approved eye protection devices such as goggles are always worn inside the
laboratory to_________.
a. Improve our vision
b. avoids eye strain
c. Protect ourselves from heat and chemicals
d. serves as the design and props inside the lab
10. Laboratory activity should start immediately upon entering the laboratory to end the
activity on time even if the teacher is not yet around.
a. True
b. False

Lesson Laboratory Apparatus and Equipment Used in

1 Biotechnology

Biotechnology is technology that utilizes biological systems, organisms or parts of this to

develop or create different products.

Brewing and baking bread are examples of processes that fall within the concept of
biotechnology to produce the desired product. Such traditional processes usually utilize
the organisms in their natural form (or further developed by breeding), while the more
modern form of biotechnology will generally involve a more advanced modification of the
biological system or organism.

This will not be possible without the help of laboratory apparatus or equipment to ensure
the quality of the product.

What’s In

Unscramble the words that would describe the picture below and Write your answer on
the space provided.

A L B O R Y R A O T

Source:https://constellations.uk.com/case-
studies/ntu-brackenhurst-science/

S I C E N T S T I

Source: https://wbckfm.com/
 E M D I N C I E S

Source: https://www.nst.com.my/lifestyle/heal/2020/05/591520/cmco-dont-miss-out-medicine

S I C E N C E

L C A S S

Source:https://www.timeshighereducation.com/news/a-level-changes-criticised-by-scientific-
community/2012594.article

What do you think is the relationship of these words to Laboratory apparatus and
equipment? Write your answer below.

What’s New

Word Search. Find the words that are associated to Laboratory apparatus and equipment
listed below in the word search box and encircle them.
Locate the given words in the grid; directions can be horizontally, vertically, or
diagonally.
 What Is It

Laboratory Apparatus and Equipment

Laboratory equipment and apparatus are very important in the field of biotechnology.
It assures that the product produce is with high quality.
If you work in a laboratory setting, you undoubtedly encounter many kinds of expensive
and complicated instruments and machines. Knowing how to use these tools can only
benefit you. This is especially true if you are expected to use them while pursuing your
area of research and testing. Not knowing what you are doing can set you back in a
variety of ways. There are different apparatus and equipment used in biotechnology. Here
are some.

Autoclave
A heated and
pressurized container used
to sterilize equipment and
kill any organisms left.
 Water bath
Heats items up to a
specific temperature and
keeps it at that temperature.

Hot Plate
An electrical device
used to heat things up.

Compound Light
Microscope
An instrument used to
see things not visible to the
naked eye

Incubator
An apparatus used to
control the environment to
culture and grow bacteria and
hatching eggs artificially or to
provide a stable environment for
a reaction.
Micropipette
Use to measure exact
amounts of small liquids.

Graduated Cylinder
Use to measure
liquid volume, very
accurate.

Forceps
They are used to grip
objects that are too small
for your fingers or if they are
extremely hot.

Centrifuge
An apparatus
consisting essentially of a
compartment spun at high
speeds to help separate
liquids from solids.

Pestle and Mortar

Used to crush and
mix solids
 Beaker
Used to heat, stir,
and measure liquids.

Electronic Balance/Digital
Scale
Use to measure
small amounts of weight.

Thermometers
Used to measure
temperature in ⁰F or ⁰C.

Goggles
Used to protect eyes
during laboratory
experiments, must be worn
at all times.
Beaker Tongs
Use to hold beakers
that are extremely hot.

Ring Stand
Use to hot beakers,
and other items that are being
heated.

Laminar Air Flow Chamber

Use to prevent
contamination of
semiconductors wafers,
biological samples, or any
particle sensitive device.

Fume Hood

A local ventilation
unit used to limit exposure
to toxic fumes or dusts.
 Micro centrifuge Tubes

Use to hold small

measures of liquids to be
used in a centrifuge.

Electrophoresis Chamber

A chamber used in
gel electrophoresis that
conducts an electrical
current.

Storage Container
Used to hold and
store chemicals and other
solids or liquids. It should
be labelled correctly.

Test Tube

Used to hold, mix,

heat small amounts of
solids or liquids.
Funnel
Use to channel
liquids or fine-grained solids
without spillage.

Distiller

Used to mix or
agitate solutions.

Rubber Aspirator
Used in all different
types of experiments and its
main purpose is for moving
air or liquids.

Erlenmeyer flask

Used for mixing and

storing chemicals and
solutions.

Lab Coat

Use to protect your

clothing during laboratory
experiments from splashing
chemicals and other
solutions.
 Gloves
Use to protect your
hands during laboratory
experiments.

Face Shield

Use to protect your face

from heat and splashing
chemicals in the lab.

Burette

Use to dispense known

amounts of a liquid reagent
in experiments.

Pictures retrieved from https://prezi.com/ra68hx03abrk/30-laboratory

 What’s More

ACTIVITY #1 Which is Which?

Encircle the function of the following equipment based on the picture.

Use to stir/mixed solution Use to limit the exposure to dust

Used to protect your nose from Use to protect your eyes from
chemicals chemicals

Mixed small amount of liquids Helps separate liquid from solid

 Use to prevent contamination of Mixed small amount of liquids
semiconductors wafers, biological
samples, or any particle sensitive
device

Use to protect hands during the Use to dispense amounts of a

experiment liquid reagent in experiments

Use to protect hands during the Use to transfer chemicals from

experiment one place to another

Source:https://www.google.com/search?q=distiller+apparatus&sxsrf=ALeKk03D4eGlf5WhdUXc
6SjS1CHr6g5Omw:1594278160887&source=lnms&tbm=isch&sa=X&ved=2ahUKEwjSps_7zL_qA
hU5yYsBHQG2AIkQ_AUoAXoECBIQAw
 What I Have Learned

ACTIVITY 2: Fill in the blanks to complete the given statements. Choose from the words
inside the box and write in on the space provided.
INCUBATOR FUNNEL

_________________An apparatus used to control the environment to

1-2. culture and grow bacteria and hatching eggs artificially or to provide a
stable environment for a reaction.

_________________Use to channel liquids or fine-grained solids without

spillage.

GRADUATED CYLINDER MICROPIPPETTE

_________________ Used to measure exact amounts of small liquids. WHILE

3-4.
_________________ Used to measure liquid volume, very accurate.

MORTAR AND PESTLE FORCEPS

_________________They are used to grip objects that are too small for
5-6. your fingers or if they are extremely hot while ______________ used to
crush and mix solid

IRON STAND WITH RING BUNSEN BURNER

_________________Use to hot beakers, and other items that are being

7-8. heated. WHILE _________________A natural gas powered piece of
equipment that is used to heat, sterilization or combustion.
 What I Can Do

Goal
Express one’s idea on the importance of laboratory apparatus and equipment
Product
Make a photo diary about what they have learned from the lesson
Criteria Presentation of Ideas 5 points
Content 5 points
Creativity 5 points
TOTAL 15 points

PHOTO DIARY

Put a photo or cut-out that

would best describe what you
have learned from this module

Write 1-2 sentences that will explain the photos or

drawing that you use.

Downloaded and made from Canva.ph

 Assessment

Below are letters which have been jumbled. Struggle with the letters by arranging them
to form the word/s that is described.

I M O C R E G F U E C N I T

____________________________1 Used to hold small measures of

. liquids to be used in a centrifuge.

R D O P E R P

_____________________________2. Used to measure small amounts of liquid for

transfer.

H T E R M O M E E R T

_____________________________3. Used to measure temperature in ⁰F or ⁰C.

B L A O C T A

____________________________4. Used to protect your clothing during laboratory

experiments from splashing chemicals and other
solutions.
 Additional Activities

List other laboratory apparatus used in biotechnology which are not being
discussed above. Describe each and write their use.
 Answer Sheet

Module 1: Lesson 1: Laboratory apparatus/equipment and their Uses

What I Know What’s In

1. 6. 1.
2. 7. 2.
3. 8. 3.
4. 9. 4.
5. 10.
.
What’s New What’s More

What I Have Learned Assessment

1. 1.
2. 2.
3. 3.
4. 4.
5.