Standard practices include BSL-2 plus: + strictly controlled access to the lab; * specific training for lab personnel in handling potentially lethal agents; + decontaminating all waste. + changing contaminated protective lab clothing, decontaminating lab clothing before laundering; * institutional policies regarding specimen collection and storage from workers to establish exposurePathogenic, cause serious disease Effective treatment and preventive measures usually available Little person-to- person spreadMDR - TB , XMDR-TB AND BIOSAFETY With growing incidences of MDR-TB and XMDR-TB itis Te NMCSScut elie Microbiology laboratories must install to PURO ICCC) Liiictei Cela a ae 1A precaution's are not taken a Lica) 7-3 infected with grave Coola ote TreoTB DIAGNOSTICS AND LABORATORY STRENGTHENING fete gE RUB U -1e0 CCCR UT UE Rell el LA SSU Ce le Ney obtained by growing and identifying Mycobacterium tuberculosis from clinical specimens and conducting DST of the organism to confirm or exclude Cle Laboratory infrastructure, appropriate biosafety measures and maintenance Equipment validation and maintenance Specimen transport and referral en Mae ee SRO oy Tere COCO We Cue ey Cue eo ose management system are a priority.* Agents with potential for respiratory transmission, may cause serious and el A CUE lero} * May be studied at BSL-2 for diagnosis = Agents include 1 yooacterium ee eons Rola ne sir RH aaa mt ho eM Ueno e 0CCC RCL human-derived fol (eTele Pm eLoLol VVC SI SIV od TMU AMT Se-e CON Ee Agents include measles Neto species, pathogenic Uf) o)o) Liye) Pan okra a /0 m botulinum, hepatitis Cats+ Standard practices required: frequent handwashing door that can be kept closed when working; limits on access to the lab space when working; no smoking, eating, drinking, storage of food in laboratory; care to minimize splashes and actions that may create aerosols (tiny droplets); decontamination of work surfaces after every use after any RoRAgents not known to cause CIT recom Ame UN AEC LCS + Some organisms may cause disease in immunocompromised individuals Agents include Bacil/us Ea em elated gruberi, infectious canine rR ra ola pathogenic F. co/i speciesCDC/NIH: BIOSAFETY LEVEL 3 AND LEVEL 4.CDC/NIH: BIOSAFETY LEVEL 1 AND LEVEL 2.18 ee Nn Peete een ered Cua) Renesas Ege Sa Recor eat Pea oy DPE Pecan Terria practices era el Perales Creme er ee ees eekly Prarie Perea ane ReceReecn Conte ee Coa directional airflow Pee nUy Ei ece tes ro tae eresgee iat None eee URLS foie U gas} biological safety cabinet for potential Eee Pokies ene Re primary devices for all activities Class III biological safety cabinet, Pee eee era) autoclave (through the wall), filtered airTite Te CMO M aN 1 cn me CO) Pau WES CM ac cee Een CL meen) Year Require that all equipment or eeu RUS) handled to prevent ETSI eu Rem Cael agents SS Ter ee ce om require additional precautions See aa Recor site decontaminationSECONDARY BARRIERS: Secondary barriers: structural aspects of the laboratory that make working environment safer against infection Sinks for handwashing, special containment areas, special air NEU Cn)BARRIERS aaa CLES physical barriers or personal protective equipment between lab worker and pathogen ¢ Gloves, masks, special breathing Fle) te Weer* Labs divided into 4 biosafety levels; protective practices increase with each eee emma (CaN (crt dangerous agents, require fewest precautions Beem MCL mem TNC Ue] methods because dealing with agents that are most dangerous to human health* Precautions so people researching or trying to identify organisms do not become infected * While handling or testing clinical specimens, workers could accidentally infect themselves or coworkers * Labs must adhere to very specific safety regulations to work with organisms that pose a threat to human healthSTANDARD MICROBIOLOGICAL PRACTICES - NOT permitted in Ey Dole-\l aU PTT Smoking OE LLC Pipetting by mouth Wi SUL] CCCI Tec Handling contact lensesWHY IS BIOSAFETY IMPORTANT? Teron meee nA} hazards of processing infectious agents Guidelines developed om yee mL RII) microbiological and medical labs through engineering controls, Tue tenn ee work practicesBIOSAFETY: PREVENTING LAB-ACQUIRED INFECTIONS BE TeCucy Viruses Fungi Human blood, unfixed LSS) Human cell lines Recombinant DNA=)(eyy Va ie Ih Dr.T.V.Rao MD