Alfie Lyn C. Pedregosa 11- St.
Ambrose
EFFECT ON REACTION
FACTORS
RATE IF FACTOR IS
REDUCED/DECREASED
When all of the enzymes in the system
bind to the substrate, the subsequent
substrate molecules will have to wait
1. Enzyme Concentration until the enzyme becomes available
once the reaction is done. This means
that the reaction rate will decrease
when the enzyme concentration also
decreases.
The enzymes have a lower chance of
2. Substrate contacting the substrate when the
Concentration concentration is low and the activity or is accessible to bind and conduct
rate of reaction is low.
If the reaction rate factor is reduced at
low exposure levels, the inhibitor
3. Inhibitor Concentration significantly competes for the enzyme.
GENERAL BIOLOGY- WEEK 6
EFFECT ON REACTION
RATE IF FACTOR IS
RAISED/INCREASED
As long as there is substrate to bind
to, increasing enzyme concentration
will speed up the reaction. The
reaction will no longer speed up
once all of the substrate has been
bound, as there will be nothing for
additional enzymes to bind to.
The enzyme becomes saturated with
substrate as the concentration of
substrate increases. More substrate
reaction as soon as the catalytic site
is empty.
The inhibitor's efficacy is
significantly reduced at high
substrate concentrations because
the substrate outcompetes it due to
its higher concentration.
October 8,2021
BRIEF EXPLANATION AS TO WHY
THESE FACTORS AFFECT ENZYME
FUNCTION AND REACTIONS
RATES IN THAT WAY
The concentration and activity of
enzymes are directly proportional.
Whether the substrate concentration
is less than the enzyme
concentration, hence lowering the
enzyme concentration has no effect
on enzyme activity.
If there is enough substrate, enzymes will
work optimally. As the concentration of
substrate increases, so does the rate of
enzyme activity. However, the rate of
enzyme activity is unlikely to increase
indefinitely. Because there is so much
substrate, the enzymes will eventually
become saturated, and no additional
substrates will fit at the same time.
As a result, the substrate has been
unable to connect to the same active
site.

If this is not possible, lowering the
temperature can be used to reduce the
4. Temperature number of collisions and the kinetic
energy available for activation energy.
Reducing the size of the container: This
places reactant closer to each other and
5. Size of Container they are more likely to encounter each
other and react.
 
As pH decreases, the concentration of
hydrogen ions increases (the
environment becomes more acidic).
6. pH
As the temperature increases, so
does the vibrational energy, which
strains the bonds in the enzyme
and substrate structures.
As the size of the container
increases, more particles are
exposed to the other reactant.
There is a greater chance of
particles colliding, resulting in
more successful collisions per
second.
As the pH rises, the structure of
the enzyme's active site changes,
resulting in a significant reduction
in activity.
The rate of enzyme activity increases as
the temperature rises. Aside from that,
when the enzyme is at its optimal
temperature, it reaches the saturation
point. As the temperature rises, the active
site of the enzyme changes shape,
resulting in a significant decrease in
activity. In fact, it’s been denatured for
quite some time.
It has an impact on their evaluation.
With the same enzyme quality and
substrate concentrations, the initial
reaction rate is the same in any size
container.
The structure of an enzyme's active
site is affected by pH changes. Aside
from that, each enzyme is most
effective at a specific pH level. Truly,
the optimal pH for an enzyme is
determined by the environment in
which it operates.