Endogenous Anaerobic

Bacteroides fragilis
“ n on -he mol y ti c i n AN A BAP, k an amy ci n ,
v an comy ci n an d col i s ti n re s is tan t”

Prevotella spp.
“ fl u ore s ce bri ck re d, v an comy ci n res i s tan t,
grows on KV L B ”

Porphyromonas spp.
“ f l u ores ce bri ck re d, v an comy ci n se ns i ti v e , wi l l
n ot grow on KV L B

Mobiluncus spp.
“age n t of bacte ri al v agi n os is ”

Propionibacterium
“as s oci ate d wi th acn e f ormati on ”

Obligate
“ doe s n ot grow i n (+ ) O2 , pre v ote l l a,
fu s obacte ri u m, bacte roi de s ”
Aerotolerant
“ growth on ei th e r re du ce d O 2 or an ae robi c,
acti n omy ce s , bi fi dobac te ri u m, cl os tri di u m ”
Facultative
“ capabl e of growth in O2 an d an ae robi c ”
Specimens of Choice
“ bi l e , bl ood, bon e marrow, CS F, de cu bi tu s
u l ce r, joi n t f lu i d, pe ri ton e al f l u i d, s u l fu r
gran u l es , ti ss u e f rom bopi s y , u rin e f rom
su prapu bi c as pi rati on ”
Collection
“ ti ss u e bi ops y or as pi rati on by ne e dl e an d
sy ri n ge ”
Transport Systems
“ ru bbe r s toppe re d col l e cti on v i al (li qu i d),
ox y ge n f re e col l e cti on tu be (s wab), se l f -
con tai n i n g an ae robi c bag (ti ss u e ) ”
Anaerobic Bacteria
Culture Media
“ an ae robi c bl ood agar, f us tobac te ri u m se l e cti v e
agar, k an amy ci n v an comy ci n l ak e d bl ood,
an ae robi c ph en y e th y l al coh ol agar,
th i ogl y col l ate broth , cy cl os e ri ne ce f ox i ti n
f ru ctos e agar, bacte roi de s gin gi v al i s bl ood
agar, e gg y ol k agar, cadmi u m s ul f ate f lu ori de
acri di n e try pti cas e agar, bacte roi de s bi l e
e s cul i n agar, l actoba ci l l u s s el e cti v e me di a,
pe ptos tre ptoco ccu s se l e cti v e me di a, pe pton e
y e as t ex tract glu cos e broth ”
Anaerobic Chambers
“ gl obe box , gl ov e l e ss box (catal y s t, des s i can t,
h y droge n gas , co2 gas , ni troge n gas ,
in di cator ), an ae robi c jars , an ae robi c bags (ty pe
A bi obag, gas pak , an ae robi c pou ch ) ”
Anaerobic Gram (+) Bacilli
“ cl os tri di u m (s pore f orme rs ), bi f i dobacte ri u m,
eu bacte ri u m, l actob aci l l u s , acti n omy ce s ,
mobi l u n cu s , propi on i bac te ri u m (n on -
s pore f orme rs ) ”
Anaerobic Gram (-) Bacilli
“bacte roi de s f ragi l i s grou p, porph y romon as ,
pre v ote l l a, fu s obacte ri u m”
Anaerobic Gram (+) Cocci
“ caprococcu s , pe ptos tre ptoco ccu s n i ge r,
pe ptos tre pto coccu s ”
Anaerobic Gram (-) Cocci
“ aci dami n ococcu s f e rmen tan s , me gas ph ae ra
e ls de n i i , v e il on e l l a ”
Clostridium tetani
“ tack h e ad baci l l us , ex te n si v e s warmi n g on
BRU /BA pl ate , te rmi n al s pore , te tan u s ”
Clostridium botulinum
“ cu l ti v ati on sh ou l d be in re f e re n ce l abora tory ,
botu l i n , i nf an t botu l i s m (con tami n ate d h one y ),
wou n d botu l i s m (f oodborn e ) ”
Clostridium perfringens
“ box -car s h ape d, caps u l ate d, ce n tral or
s u bte rmi n al s pore s , cl os tri di u m wel ch i i , re v e rs e
CAMP te s t, al ph a tox i n ”
Clostridium difficile
“ f l u o r e s c e o n B R U / B A u nd e r U V li g ht , ho r s e
s t ab l e co lo r , T o x i n A ( e n t e r o t o xi n ) , T o x i n B
( c y t ot o x i n ) , C C F A , Mo d i f i ed C y c lo s e r i n e M a n n i to l
A g a r , M o d i f i e d C y c l o s e r i n e M a n n i to l B A ”