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    Inciso A

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    Adriian Fabiian
    1. The document contains data from Eadie-Hofstee plots analyzing the inhibition of an enzyme by two different inhibitors, labeled SIN and CON. 2. Linear regression analysis of the Eadie-Hofstee plots was used to determine the kinetic parameters rmax, Km, and Ki for each inhibitor. 3. For the SIN inhibitor, rmax = 6.4188 mmol/L*min, Km = -5.548 mmol/L, and Ki = 5.83977255 mmol/L. For the CON inhibitor, rmax = 5.135 mmol/L*min, Km = -5.548 mmol/L.

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    Inciso A

    Uploaded by

    Adriian Fabiian
    7 views3 pages
    1. The document contains data from Eadie-Hofstee plots analyzing the inhibition of an enzyme by two different inhibitors, labeled SIN and CON. 2. Linear regression analysis of the Eadie-Hofstee plots was used to determine the kinetic parameters rmax, Km, and Ki for each inhibitor. 3. For the SIN inhibitor, rmax = 6.4188 mmol/L*min, Km = -5.548 mmol/L, and Ki = 5.83977255 mmol/L. For the CON inhibitor, rmax = 5.135 mmol/L*min, Km = -5.548 mmol/L.

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    1. The document contains data from Eadie-Hofstee plots analyzing the inhibition of an enzyme by two different inhibitors, labeled SIN and CON. 2. Linear regression analysis of the Eadie-Hofstee plots was used to determine the kinetic parameters rmax, Km, and Ki for each inhibitor. 3. For the SIN inhibitor, rmax = 6.4188 mmol/L*min, Km = -5.548 mmol/L, and Ki = 5.83977255 mmol/L. For the CON inhibitor, rmax = 5.135 mmol/L*min, Km = -5.548 mmol/L.

    Copyright:

    © All Rights Reserved
    Download as XLSX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as xlsx, pdf, or txt
    7 views3 pages

    Inciso A

    Uploaded by

    Adriian Fabiian
    1. The document contains data from Eadie-Hofstee plots analyzing the inhibition of an enzyme by two different inhibitors, labeled SIN and CON. 2. Linear regression analysis of the Eadie-Hofstee plots was used to determine the kinetic parameters rmax, Km, and Ki for each inhibitor. 3. For the SIN inhibitor, rmax = 6.4188 mmol/L*min, Km = -5.548 mmol/L, and Ki = 5.83977255 mmol/L. For the CON inhibitor, rmax = 5.135 mmol/L*min, Km = -5.548 mmol/L.

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    of 3

    Cs 1.

    46 microMol/L SIN INHIBIDOR


    Eadie-Hofstee
    r/Cs
    CS (mmol/L) r SIN (mmol/L*min) r CON (mmol/L*min) 0.522388059701
    6.7 3.5 2.8 0.714285714286
    3.5 2.5 2 0.882352941176
    1.7 1.5 1.2
    rmax =
    Km=

    Eadie-Hofstee SIN
    4

    3.5
    f(x) = − 5.547992467713 x + 6.41878141820357
    R² = 0.99854122553219
    3 E
    2.5 3

    2 f(x) = − 5.54799246771
    2.5 R² = 0.99854122553219
    1.5
    2
    1

    0.5 1.5

    0
    0.5 0.55 0.6 0.65 0.7 0.75 0.8 0.85 0.9 0.95 1

    0.5

    0
    0.4 0.45 0.5
    SIN INHIBIDOR CON INHIBIDOR
    Eadie-Hofstee Eadie-Hofstee
    r r/Cs r
    3.5 0.41791045 2.8 rmaxi = rmax/1+(Ci/Ki)
    2.5 0.57142857 2
    1.5 0.70588235 1.2 Ki = 5.83977255

    6.4188 rmax = 5.135


    -5.548 Km = -5.548

    Eadie-Hofstee CON

    f(x) = − 5.547992467713 x + 5.13502513456285


    R² = 0.99854122553219

    4 0.45 0.5 0.55 0.6 0.65 0.7 0.75


    max/1+(Ci/Ki)

    mmol/L

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