For the exclusive use of Chem 153L class in Xavier-Ateneo EXPERIMENT 8

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Expt. 8 Urine Analysis

LAB REPORT Member 1 Member 2 Member 3 Member 4 Member 5

I. SCORE
Pre-lab Data
Data and Observations
Post-lab Questions
Sub-total
Participation
Online Pre-lab Quiz
Total Score

Objectives

II. Apparatus: III. Symbols of Chemicals:

IV. Procedure: (Note: Write schematic diagram on the sheet provided.)

V. Data and Observations

A. PHYSICAL CHEMISTRY OF URINE
SAMPLE USED COLOR ODOR TEMPERATURE
Fresh Urine
Urine after 1 to 2 hrs.

B. NITROGENOUS CONSTITUENTS OF URINE

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 For the exclusive use of Chem 153L class in Xavier-Ateneo EXPERIMENT 8

1.Urea
Formation of Biuret
CONDITION RESULTS
Formation of biuret-urea in a test tube, heated
carefully in a low flame added red litmus paper
at the mouth of the tube.
Continued heating until mass solidify
cooled and added with 10% NaOH + 0.1% CuSO 4

2. Uric Acid
Murexide Reaction
CONDITION RESULTS

Uric acid powder + 2-3 drops conc. HNO 3, Color

evaporated carefully to dryness on a water
bath or over a low flame

cooled solution + 1 drop diluteNH4OH Color

warmed Color?
What is this color due to?

3. Creatinine
Nitroprusside Test (Weyl Test)
CONDITION RESULTS

5 mL urine + few drops of 5% aqueous soln.

of nitroprusside + NaOH until alkaline

4. Indican
Obermeyer’s Test
CONDITION RESULTS

5 mL urine 5 mL reagent+ 2-3 mL CHCl3 and

shake thoroughly

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 For the exclusive use of Chem 153L class in Xavier-Ateneo EXPERIMENT 8

C. PATHOLOGICAL CONSTITUENTS OF URINE

Test for Name of Test Results

1. Albumin a) Boiling Test

b) Nitric acid test (Heller’s)

2. Glucose Benedict’s Test

3. Acetone Bodies Sodium Nitroprusside Test

a) Acetone (Legal’s Test)

b) β-hydroxybutyric acid

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 For the exclusive use of Chem 153L class in Xavier-Ateneo EXPERIMENT 8

POST-LAB QUESTIONS

1. How is uric acid formed? What is the significance of abnormally high concentration of uric acid
in the urine? (6 pts)

2. What are the amino acids involved in the formation of creatinine? (3 pts)

3. If blood is present in your urine, what does it indicate? (6 pts)

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 For the exclusive use of Chem 153L class in Xavier-Ateneo EXPERIMENT 8

4. Give the chemical equation in the formation of indican. (4 pts)

5. Explain why people with diabetes mellitus have glycosuria? (6 pts)

6. Why is albumin not normally present in the urine? (4 pts)

7. If a urine sample from a patient gave a strongly positive test for both glucose and ketone bodies,
what diagnosis might be made? Explain. (6 pts)

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8. Illustrate the urea cycle. Briefly explain the process. (5 pts)

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 For the exclusive use of Chem 153L class in Xavier-Ateneo EXPERIMENT 8

Schematic Diagram of Procedure

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