LESSON 2: BULK TRANSPORT: ENDOCYTOSIS AND EXOCYTOSIS

In addition to moving small ions and

molecules through the membrane, cells also
need to remove and take in larger molecules
and particles. Some cells are even capable of
engulfing entire unicellular microorganisms. You
might have correctly hypothesized that the
uptake and release of large particles by the cell
requires energy. A large particle, however,
cannot pass through the membrane, even with
energy supplied by the cell. Some molecules or
particles are just too large to pass through the
plasma membrane or to move through a
transport protein. So cells use two other active
transport processes to move these
macromolecules (large molecules) into or out of
the cell. Vesicles or other bodies in the
cytoplasm move macromolecules or large
particles across the plasma membrane.

There are two types of vesicle transport,

endocytosis, and exocytosis (illustrated in
the Figure below). Both processes are active
transport processes, requiring energy.

What does a cell "eat"?

Is it possible for objects larger than a small molecule to be engulfed by a cell? Of course it is. This image
depicts a cancer cell being attacked by a cell of the immune system. Cells of the immune system consistently
destroy pathogens by essentially "eating" them. Find out below how this happens.

The term ‘Endocytosis’ was given by Christain de Duve in the year 1963. Both the terms refers to the intake of
material through the plasma membrane by forming vesicles which are a membrane-bound droplet located
inside the cytoplasm of the cell. Endocytosis occurs in the animal cell and very rarely in plant cell as the plant
cell is surrounded by the cell wall, which causes hindrance in the invagination of the plasma membrane.

Transmission electron microscope image of

brain tissue that shows pinocytotic vesicles.
Pinocytosis is a type of endocytosis
 Illustration of an axon releasing dopamine by exocytosis

Endocytosis is a type of active transport that moves particles, such as large molecules, parts of cells,
and even whole cells, into a cell. There are different variations of endocytosis, but all share a common
characteristic: the plasma membrane of the cell invaginates, forming a pocket around the target particle.
The pocket pinches off, resulting in the particle being contained in a newly created intracellular vesicle
formed from the plasma membrane.

TWO TYPES OF ENDOCYTOSIS

1. PHAGOCYTOSIS

Phagocytosis (the condition of “cell eating”) is the process by

which large particles, such as cells or relatively large particles, are taken
in by a cell. For example, when microorganisms invade the human body,
a type of white blood cell called a neutrophil will remove the invaders
through this process, surrounding and engulfing the microorganism,
which is then destroyed by the neutrophil (see figure 1).

In preparation for phagocytosis, a portion of the inward-facing

surface of the plasma membrane becomes coated with a protein
called clathrin, which stabilizes this section of the membrane. The
coated portion of the membrane then extends from the body of the cell
and surrounds the particle, eventually enclosing it. Once the vesicle
containing the particle is enclosed within the cell, the clathrin
disengages from the membrane and the vesicle merges with a lysosome
Figure 1: In phagocytosis, the cell membrane
surrounds the particle and engulfs it (credit:
for the breakdown of the material in the newly formed compartment
Mariana Ruiz Villareal) (endosome). When accessible nutrients from the degradation of the
vesicular contents have been extracted, the newly formed endosome
merges with the plasma membrane and releases its contents into the extracellular fluid. The endosomal
membrane again becomes part of the plasma membrane.

2. PINOCYTOSISThis literally means “cell

drinking”. It is the intake of liquid and small
particles usually, ions, amino acids, sugars,
insulin, and lipoproteins. It was named at a
time when the assumption was that the cell
was purposefully taking in extracellular fluid. In
reality, this is a process that takes in molecules,
including water, which the cell needs from the
extracellular fluid. Pinocytosis results in a much Figure 2. The cell membrane
smaller vesicle than phagocytosis, and the invaginates, surrounds a small
volume of fluid and pinches off.
vesicle does not need to merge with a
(Credit: Mariana Ruiz Villareal)
lysosome (see Figure 2 below).

A variation of pinocytosis is called potocytosis. This process uses a coating protein, called caveolin, on
the cytoplasmic side of the plasma membrane, which performs a similar function to clathrin. The cavities in
the plasma membrane that form the vacuoles have membrane receptors and lipid rafts in addition to
caveolin.

The vacuoles or vesicles formed in caveolae (singular caveola) are smaller than those in pinocytosis.
Potocytosis is used to bring small molecules into the cell and to transport these molecules through the cell for
their release on the other side of the cell, a process called transcytosis.

EXOCYTOSIS is the reverse process of moving material into

a cell is the process of exocytosis. Exocytosis is the opposite
of the processes discussed in the last section in that its
purpose is to expel material from the cell into the
extracellular fluid. Waste material is enveloped in a
membrane and fuses with the interior of the plasma
membrane. This fusion opens the membranous envelope
on the exterior of the cell, and the waste material is
expelled into the extracellular space (see Figure 4). Other
examples of cells releasing molecules via exocytosis
include the secretion of proteins of the extracellular matrix
and secretion of neurotransmitters into the synaptic cleft
by synaptic vesicles.
Key Differences between Pinocytosis and Phagocytosis Figure 4. In exocytosis, vesicles containing substances fuse with the plasma membrane.
The contents are then released to the exterior of the cell. (Credit: modification of work by
Mariana Ruiz Villareal)

We already discuss that both the mechanisms pinocytosis and phagocytosis falls under the main
process called Endocytosis, given below are the key differences between them:

1. Pinocytosis is the process of ingestion of small liquid particles through plasma membrane with the help of
formation of vesicles known as pinosomes; Phagocytosis is the process of ingestion of solid particles through
plasma membrane with the help of lysosomes and phagosomes, which release enzymes for breaking the
larger particles.

2. The process of engulfing is by Invagination in pinocytosis, and by pseudopodia in phagocytosis.

3. Lysosomes do not play any role in pinocytosis, while lysosomes along with phagosomes help in digestion of
bigger particles.

4. Pinocytosis is not substrate specific, and cell takes all kind of surrounding fluids with all solutes present while,
Phagocytosis is specific in substrate transportation.

5. The purpose of pinocytosis is used for intake of materials, whereas phagocytosis is used for the defensive
purpose by engulfing the foreign particles. The vesicles formed in pinocytosis is pinosomes and that of
phagocytosis is known as phagosomes.

The particles taken in pinocytosis are enzymes, hormones, amino acids, sugars, etc. for the purpose of intake
of smaller particles; while phagocytosis is performed by neutrophils, macrophages, and protozoans for
defensive purpose and ingests dust, foreign particles, harmful bacteria, and virus.