_______________1. Crystal violet and
Escherichia coli
bile salts inhibit the growth of gram (+)
bacteria.
a. MacConkey Positive; Selective
b. MacConkey Positive; Differential
_______________2. Neutral red
differentiates lactose and nonlactose
fermenters;
a. MacConkey Positive; Selective
b. MacConkey Positive; Differential
_______________3. Test the ability of the
organism to reduce nitrate to nitrite;
a. Nitrate Redution; Positive
b. Nitrate Redution; Negative
_______________4. Nitrate broth with the
organism is tested for presence of ____and
reduction of nitrate;
a. Gas b. Bacteria c. Liquid d.
Leukocyte
_______________5. Colonize the GI tract
which is commonly associated with human
infection;
a. First Group b. Second Group
_______________6. Colonizer but rarely
associated with infection; They are
environmental inhabitants or colonizers of
other animals;
a. First Group b. Second Group
_______________7. Edwardisiella tarda
a. First Group b. Second Group
_______________8. Yersinia pestis
a. First Group b. Second Group
_______________9. Yersinia enterocolitica;
a. First Group b. Second Group
_______________10. Yersinia
pseudotuberculosis;
First Group b. Second Group
II. A. Lactose Fermenters; B. Non Lactose
Fermenters;
C. Late Lactose Fermenters; (FIRST grp)
_______________11. Citrobacter spp.
_______________12. Morganella spp.;
_______________13. Klebsiella spp.;
_______________14. Enterobacter spp;
_______________15.
_______________16. Salmonella
_______________17. Shigella
_______________18. Proteus spp.
_______________19. Serratia spp.
_______________20. Providencia spp.
_______________21. Can cause UTI’s and
septicemia ▪ Diarrhea and gastroenteritis
disease syndromes;
a. Escherichia coli b. Serratia spp.
_______________22. Virulence factors/
Disease: Also known “Montezuma’s
revenge” or “turista”
a. Enteroinvasive e.coli b.Enterotoxigenic
E.coli
_______________23. Pili; Toxins: heat-
labile (LT) and heat stable (ST)
enterotoxins;
a. E.coli: Virulence Factor b. E.coli:
Disease
________________24. Traveler’s and
childhood diarrhea (epidemic diarrhea);
a. E.coli: Virulence Factor (ETEC)
b. E.coli: Disease (ETEC)
_______________25. Only grows in blood
agar
a. Escherichia coli b. Serratia spp.
_______________26. Envades enterocytes
(intestinal epithelium) lining the large
intestine causing a shigella like infection;
a. Enteroinvasive e.coli b.Enterotoxigenic
E.coli
_______________27. Dysentery and
Bloody stools (RBC, neutrophils and
mucus);
a. E.coli: Virulence Factor(EIEC)
b. E.coli: Disease(EIEC)
______________28. Most strains are non-
motile and late or non-lactose fermenter;
a. . E.coli: Virulence Factor(EIEC)
b. E. coli: Characteritisc (EIEC)
______________29. Bundle-forming pilus
and other factors for attachment to cells of
the small bowel;
a. . E.coli: Virulence Factor (EPEC)
b. E. coli: Characteritisc (EPEC)
c. E. coli: Disease (EPEC)
______________30. Non -invasive and
does not produce toxin;
a. . E.coli: Virulence Factor(EPEC)
b. E. coli: Characteritisc (EPEC)
c. E. coli: Disease (EPEC)
______________31. Diarrhea in infants
(watery with mucus but no blood);
a. . E.coli: Virulence Factor(EPEC)
b. E. coli: Characteritisc (EPEC)
c. E. coli: Disease (EPEC)
_______________32. May be isolated in
enteric media;
a. . E.coli: Virulence Factor(EPEC)
b. E. coli: Characteritisc (EPEC)
c. E. coli: Disease (EPEC)
_______________33. Also known as
Verotoxic E.coli (VTEC);
a. EPEC b. EHEC c.EIEC
_______________34. Cytotoxin (verotoxin)
similar to Shiga toxin (S. dysenteriae);
Frequently associated with E.coli 0157:H7;
The only strain that is sorbitol negative;
a. . E.coli: Virulence Factor(EHEC)
b. E. coli: Characteritisc (EHEC)
c. E. coli: Disease (EHEC)
_______________35. Hemorrhagic colitis
and hemolytic uremic syndrome;
a. . E.coli: Virulence Factor(EHEC)
b. E. coli: Characteritisc (EHEC)
c. E. coli: Disease (EHEC)
_______________36. Actual pathogenic
mechanism not known;
a. . E.coli: Virulence Factor(EAEC)
b. E. coli: Characteritisc (EAEC)
c. E. coli: Disease (EAEC)
_______________37. Watery diarrhea;
a. . E.coli: Virulence Factor(EAEC)
b. E. coli: Characteritisc (EAEC)
c. E. coli: Disease (EAEC)
_______________38. Lactose negative and
produces no gas during fermentation; Non-
motile
a. Anaerogenic E. coli or “E. coli inactive”
b. Anaerobic E. coli or “E. coli inactive”
_______________39.Virulence Factor:
Opportunistic causing bacteremia, urinary
tract inf., respiratory tract inf. and wound
infections;
a. Eneterobacter and Klebsiella
b. Shigella
_______________40. May be encapsulated
and appears as mucoid colonies that tend to
“string”;
a. Eneterobacter and Klebsiella
b. Shigella
_______________41. Causes severe
pneumoniae that is necrotic and
hemorrhagic producing “Currant jelly-like
sputum”;
a. Shigella infection b. Klebsiella
pneumonia
_______________42. Greenish Metallic
Sheen is commonly seen in;
a. E. coli b. Shigella c. Klebsiella
_______________43. Opportunistic causing
bacteremia, septicimia, urinary tract,
respiratory tract and wound infections;
a. Critobacter and Serratia
b. E. coli
_______________44. Produces red
pigment (prodigiosin) when incubated at
room temperature;
a. Gelatinase
b. Serratia marcescens
_______________45. Protect organism in
stomach acids; Promote attachment and
phagocytosis by intestinal mucosal cells;
a. Salmonella b. Shigella
_______________46. Associated with S.
typhimurium and S. enteritidis;
a. Gastroenteritis and diarrhea
b. Bacteremia and extra intestinal infections
_______________47. Associated with S.
choleraesius and S. Dublin;
a. Gastroenteritis and diarrhea
b. Bacteremia and extra intestinal infections
_______________48. Associated with S.
typhi and S. paratyphi • Isolated in blood at
(weeks 1 to 2), in urine (weeks 3 and 4) and
indefinitely in stool;
a. Enteric fever (typhoid fever, or typhoid)
b. Bacteremia and extra intestinal infections
_______________49. Appears as metallic
colonies with a black ring in Bismuth sulfite
agar;.
a. Shigella b. Salmonella
______________50. Diagnosed with
Widal’s test in reference laboratories;
a. Shigella b. Salmonella
______________51. Mediate adherence
and invasion of mucosal cells, escape from
phagocytic vesicle, intercellular spread;
a. Shigella b. Salmonella
_____________52. Produces neurotoxin
and enterotoxin;
a. S. aureus b. S. dysenteriae
_____________53. Shigellosis or bacillary
dysentery;
a. Bloody and mucoid stools
b. Watery diarrhea
_____________54. Has a characteristic
“swarming motility” on blood and chocolate
agar and has rapid urease activity;
a. Morganella b. Providencia c. Proteus
_____________55. Opportunistic causing
GI and wound infection;
a. Edwardshiella tarda
b. Yersinia pestis
_____________56. Isolated from cold
blooded animals;
a. Edwardshiella tarda
b. Yersinia pestis
_____________57. Adapt for intracellular
survival by production of antiphagocytic
capsule, exotoxins, endotoxins, coagulase
and fibrinolysin;
a. Edwardshiella tarda
b. Yersinia pestis
_____________58. DISEASE: Bubonic
Plaque; Pneumonic Plaque;
a. Edwardshiella tarda
b. Yersinia pestis
_____________59. Exhibit a rough,
cauliflower appearance at 48 hrs in SBA;
Exhibit a “stalactite pattern” in broth culture;
a. Edwardshiella tarda
b. Yersinia pestis
____________60. Allows organism to
attach and invade and spread to lymphatic
tissue;
a. Yersinia enterocolitica and Y.
pseudotuberculosis
b. Y. pestis
____________61. Disease associated with
Yersinia enterocolitica and Y.
pseudotuberculosis;
a. Pneumonic plaque b. Enterocolitis
____________62. Bull’s eye colonies in CIN
agar after 48 hours incubation for;
a. Yersinia enterocolitica b.Y.
pseudotuberculosis
III.
____________63. Somatic antigen;
a. O b. K c. H
____________64. Envelope antigen;
a. O b. K c. H
____________65. Vi antigen for
Salmonella;
a. O b. K c. H
____________66. flagellar antigen;
a. O b. K c. H
____________67. Heat stable and is the
lipopolysaccharide of the cell wall;
a. O b. K c. H
____________68. Composes the endotoxin
portion;
a. O b. K c. H
____________69. Heat labile and consists
of capsular polysaccharide that surrounds
the cell wall;
a. O b. K c. H
____________70. Inactivated by heating at
100C for 10 minutes to detect the O
antigen;
a. O b. K c. H
____________71. Found in motile
members;
a. O b. K c. H
____________72. Heat labile and used to
serotype within species of salmonella;
a. O b. K c. H
____________73. Selects gram negative
bacteria and also differentiates lactose
fermenters (pink-red) from non-lactose
fermenters (colorless);
a. MacConkey b. Eosin Methylene Blue
____________74. – Inhibit gram (+)
bacteria;
a. Bile Salts, Neutral red and crystal violet –
b. Lactose
c. Neutral red
____________75. Carbon source;
a. Bile Salts, Neutral red and crystal violet –
b. Lactose
c. Neutral red
___________76. – brown in pH 6.8 to 8.0
and pink-red at pH less than 6.8;
a. Bile Salts, Neutral red and crystal violet –
b. Lactose
c. Neutral red
___________77. Selects gram negative
bacteria and also differentiates lactose
fermenters (purple color to “green metallic
sheen) from non-lactose fermenters
(colorless);
a. MacConkey b. Eosin Methylene Blue
___________78. Selects for stool
pathogens by inhibiting normal flora
coliforms and also differentiates lactose
fermenters (yellow/orange) from non-lactose
fermenters (green);
a. HE Agar b. XLD Agar
____________79. inhibit gram (+) bacteria
and gram (-) coliforms;
a. Bile salts (high amounts) b. Brothymol
blue;
_____________80. Carbon source in HE
Agar;
a. Lactose, sucrose and salicin
b. Xylose
____________81. Indicator in HE Agar;
a. Phenol red
b. Bromthymol blue
____________82. inhibit gram (+) bacteria,
gram (-) coliforms and swarming of Proteus
in XLD Agar;
a. Xylose
b. Sodium deoxycholate
____________83. Carbon source in XLD
Agar;
a. Xylose
b. Sodium deoxycholate
____________84. Indicator in XLD Agar;
a. Phenol red
b. Bromthymol blue
____________85. Selects for stool
pathogens by inhibiting normal flora
coliforms and also differentiates xylose
fermenters (yellow) from non-xylose
fermenters (red);
a. HE Agar b. XLD Agar
____________86. Selects for stool
pathogens by inhibiting normal flora
coliforms and also differentiates lactose
fermenters (pink-red) from non-lactose
fermenters (clear);
a. Salmonella-Shigella Agar
b. HE Agar
____________87. Indictor in Salmonela-
Shigella Agar
a. Phenol red b. Neutral red
____________88. Also known as Yersinia
Selective Agar; Selective media for Yersinia
species;
a. Salmonella-Shigella Agar
b. Cefsulodin-irgasan-novobiocin
____________89. Removes amino group
from each amino acid;
a. Deaminase reactions
b. Decarboxylase reactions
____________90. Removes carboxyl group
from specific amino acid;
a. Deaminase reactions
b. Decarboxylase reactions
____________91. Test for the ability of an
organism to split tryptophan to form the
compound indole;
a. Indole Production (KOvac’c Method)
b. Spot Indole Test
____________92. Development of a blue
color within 20 seconds; POSITIVE for
Indole Production Test;
a. E. coli
b. Klebsiella and Enterbacter
____________93. No color development or
slightly pink color; NEGATIVE for Indole
Production Test
a. E.coli
b. Klebsiella and Enterbacter
____________94. Determines the ability of
the microorganism to Ferment glucose;
a. Methyl Red/ Voges-Proskauer Test
b. Indole Production Test
_____________95. Add 5 or 6 drops of
methyl red per 5 ml broth;
a. a. Methyl Red Test
b. Voges-Proskauer Test
_____________96. Add 6 drops of α-
naphtol and 2 drops of KOH to 1 ml broth,
expose to O2 and stand for 10-15 mins;
a. a. Methyl Red Test
b. Voges-Proskauer Test
____________97. Determine the ability of
an organism to use sodium citrate as the
sole source of carbon (sodium citrate →
ammonia)  Indicator: Bromthymol blue;
a. Citrate Utilization b. Sodium Utilization
____________98. Test the enzymatic ability
of an organism to decarboxylate an amino
acid to form an amine;
a. Deaminase Reaction
b. Decarboxylase (Moeller’s Method)
____________99. Test whether a gram-
negative rod utilizes glucose, and
lactose/sucrose with phenol red as
indicator;
a. TSI b. LIA c. SIM
____________100. Determines the ability of
the microorganism to decarboxylate or
deaminate lysine and form H2 S;
a. TSI b. LIA c. SIM
____________101. Determines the ability of
the microorganism to form H2 S, split
tryptophan to form the compound indole,
and observe for motility;
a. TSI b. LIA c. SIM
_____________102. Test the ability of an
organism to produce the enzyme urease
which hydrolyzes urea; Indicator is phenol
red;
a. Sulfide Indole Motility Test
b. Urea Hydrolysis (Christensen’s Method)
_____________103. Rapid urease
producer;
a. Proteus and Morganella b. Klebsiella
pneumonia
_____________104. Slow urease producer;
a. Proteus and Morganella b. Klebsiella
pneumonia
_____________105. Determine the ability
of an organism to use acetate as the sole
source of carbon;
a. Acetate Utilization b. Esculin Hydrolysis
_____________106. Determine the ability
of an organism to hydrolyze the glycoside
esculin;
a. Acetate Utilization b. Esculin Hydrolysis
_____________107. Used to determine the
ability of an organism to produce proteolytic
enzymes that liquefy gelatin;
a. Esculin Hydrolysis b.Gelatin Hydrolysis
_____________108. To determine if an
organism is motile;
a. Motility Testing b. Gelatin Hydrolysis
_____________109. Detection of late
lactose fermenters;
a. ONG Test b. ONPG Test c. OF
Medium
_____________110. Test the ability of the
organism to use carbohydrates substrates
(glucose) to produce acid by products;
a. ONG Test b. ONPG Test c. OF
Medium
______________111. Test the ability of an
organism to oxidatively deaminate
phenylalanine to phenylpyruvic acid
(detected by 10% ferric chloride);
a. Phenylalanine Deaminase
b. ONPG Test