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  • Perhitungan Tonisitas 1. Metode Ekivalensi HCL

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    Familia Maya Sarii
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    The document describes 5 methods to calculate the tonicity of a solution: equivalence to HCl method, cryoscopic method, White Vincent method, Sprowls method, and dissociation factor method. Across the 5 methods, the solution was found to be hypotonic, requiring the addition of NaCl to achieve isotonicity with 0.9% NaCl. The osmolarity was also calculated and found to be 123.8819 mOsmol/L, indicating the solution is hypotonic.

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    The document describes 5 methods to calculate the tonicity of a solution: equivalence to HCl method, cryoscopic method, White Vincent method, Sprowls method, and dissociation factor method. Across the 5 methods, the solution was found to be hypotonic, requiring the addition of NaCl to achieve isotonicity with 0.9% NaCl. The osmolarity was also calculated and found to be 123.8819 mOsmol/L, indicating the solution is hypotonic.

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    42 views8 pages

    Perhitungan Tonisitas 1. Metode Ekivalensi HCL

    Uploaded by

    Familia Maya Sarii
    The document describes 5 methods to calculate the tonicity of a solution: equivalence to HCl method, cryoscopic method, White Vincent method, Sprowls method, and dissociation factor method. Across the 5 methods, the solution was found to be hypotonic, requiring the addition of NaCl to achieve isotonicity with 0.9% NaCl. The osmolarity was also calculated and found to be 123.8819 mOsmol/L, indicating the solution is hypotonic.

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    of 8

    Perhitungan Tonisitas

    1. Metode Ekivalensi HCl

    • Lidocaine

    Liso = 3,4

    E = 17 x Liso/BM = 0,247

    Kesetaraan NaCl = E x Gram zam = 0,247 x 0,024 = 0,0059

    • Epinephrine Bitartrate

    Liso = 3,6

    E = 17 x Liso/BM = 0,334

    Kesetaraan NaCl = E x Gram zam 0,334 x 0,0000024= 0,0000008

    • Potassium Metabisulfite

    Liso = 1,9

    E = 17 x Liso/BM = 0,172

    Kesetaraan NaCl = E x Gram zam = 0,172 x 0,000144= 0,00002477

    • EDTA Disodium

    Liso = 4,8

    E = 17 x Liso/BM = 0,243

    Kesetaraan NaCl = E x Gram zam = 0,243 x 0,000003 = 0,00000073

    • Sodium Chloride

    Liso = 3,4

    E = 17 x Liso/BM = 0,989

    Kesetaraan NaCl = E x Gram zam = 0,989 x 0,000492 = 0,000486

    • HP-B-CD

    Liso = 1,9

    E = 17 x Liso/BM = 0,028

    Kesetaraan NaCl = E x Gram zam = 0,028 x 0,0137 = 0,00038


    • Sodium Sacharine

    Liso = 1,9

    E = 17 x Liso/BM = 0,157

    Kesetaraan NaCl = E x Gram zam = 0,157 x 0,000108 = 0,0000148

    Total Tonisitas : 0,0068 gr / 100 ml

    NaCl 0,9% dalam 100 ml setara = 0,9/100 x 100 ml =0,9 ml

    Selisih Tonisitas = (0,9-0,0068) gram = 0,8932 gram

    Hipotonis, setara kekurangan NaCl 0,9% = 0,8932 gram

    2. Metode Krioskopik

    Tf = Liso x g x 1000 / BM x V

    • Lidocaine

    (3,4 x 0,024 x 1000) / 234,34 x 100 = 0,0035

    • Epinephrine Bitartrate

    (3,6 x 0,0000024 x 1000) / 183,204 x 100 = 0,00000047

    • Potassium Metabisulfite

    (1,9 x 0,000144 x 1000) / 188,177 x 100 = 0,00001454

    • EDTA Disodium

    (4,8 x 0,000003 x 1000) / 336,16 x 100 = 0,00000043

    • Sodium Chloride

    (3,4 x 0,000492 x 1000) / 58,44 x 100 = 0,00029

    • HP-B-CD

    (1,9 x 0,0137 x 1000) / 1135 x 100 = 0,00023

    • Sodium Sacharine

    (1,9 x 0,000108 x 1000) / 205,17 x 100 = 0,00001

    Total = 0,004
    Selisih Penurunan Tf = 0,52-0,004 = 0,496

    Setara dengan NaCl = 0,92/0,496 = 0,9/x = 0,485 gram

    Hipotonis, setara kekurangan NCl 0,9% adalah 0,485 gram

    3. Metode White Vincent

    Vol. air = (g x E) / 0,9%

    • Lidocaine

    (0,247 x 0,024 ) / 0,9% = 0,65556

    • Epinephrine Bitartrate

    (0,324 x 0,0000624 ) / 0,9% = 0,000089

    • Potassium Metabisulfite

    (0,172 x 0,000144 ) / 0,9% = 0,00275

    • EDTA Disodium

    (0,243 x 0,000003 ) / 0,9% = 0,000081

    • Sodium Chloride

    (0,989 x 0,000492 ) / 0,9% = 0,054

    • HP-B-CD

    (0,028 x 0,0137 ) / 0,9% = 0,4222

    • Sodium Sacharine

    (0,137 x 0,000108 ) / 0,9% = 0,00164

    Total = 0,47779 ml

    100 ml - 0,47779 ml = 99,5221 ml

    0,9/x = 100/99,5221 = 0,8957 gram

    Hipotonis, setara kekurangan 0,8957 gram NaCl

    4. Metode Sprowls
    V1 = (Liso x g x 1000) / (BM x 0,52)

    V2 = (V1 x g) / 0,3

    • Lidocaine

    (3,4 x 0,024 x 1000) / (234,34 x 0,52) = 0,669 X 0,024 / 0,3 = 0,535

    • Epinephrine Bitartrate

    (3,6 x 0,0000024 x 1000) / (183,204 x 0,52) = 0,00000001 X 0,0000024 / 0,3 =


    0,00000000000008

    • Potassium Metabisulfite

    (1,9 x 0,000144 x 1000) / (188,177 x 0,52) = 0,00000015 X 0,000144 / 0,3 =


    0,000000000072

    • EDTA Disodium

    (4,8 x 0,000003 x 1000) / (336,16 x 0,52) = 0,00000002 X 0,000003 / 0,3 =


    0,0000000000002

    • Sodium Chloride

    (3,4 x 0,000492 x 1000) / (58,44 x 0,52) = 0,00000955 X 0,000492 / 0,3 =


    0,00000002

    • HP-B-CD

    (1,9 x 0,0137 x 1000) / (1135 x 0,52) = 0,00000039 X 0,0137 / 0,3 = 0,00000002

    • Sodium Sacharine

    (1,9 x 0,000108 x 1000) / (205,17 x 0,52) = 0,00000009 X 0,000108 / 0,3 =


    0,0000000000324

    Jumlah yang ditempati zat = 0,053826

    Volume berlebihan = 100-0,053826 = 99,94617

    Kelebihan volume setara dengan = 0,9/x = 100/99,94817 = 0,8995 gram

    5. Metode Faktor Disosiasi

    • Lidocaine

    Konsentrasi (g/L) = 0,1


    Fa = 1,5

    Tonisitas zat = (Fa/BM) x c = 140,604

    • Epinephrine Bitartrate

    Konsentrasi (g/L) = 0,00004

    Fa = 1,8

    Tonisitas zat = (Fa/BM) x c = 0,01319

    • Potassium Metabisulfite

    Konsentrasi (g/L) = 0,0024

    Fa = 1,5

    Tonisitas zat = (Fa/BM) x c = 0,6774

    • EDTA Disodium

    Konsentrasi (g/L) = 0,0005

    Fa = 1,5

    Tonisitas zat = (Fa/BM) x c = 0,2521

    • Sodium Chloride

    Konsentrasi (g/L) = 0,082

    Fa = 1,8

    Tonisitas zat = (Fa/BM) x c = 8,6257

    • HP-B-CD

    Konsentrasi (g/L) = 0,02277

    Fa = 1,8

    Tonisitas zat = (Fa/BM) x c = 46,5191

    • Sodium Sacharine

    Konsentrasi (g/L) = 0,0018

    Fa = 1,8

    Tonisitas zat = (Fa/BM) x c = 0,6647


    Total = 197,35619

    Selisih Tonisitas = 3,2 x 0,28/197,35619 = 0,00454 gram/ 1000 ml

    Massa NaCl berlebih dalam 100 ml = 0,00454/1000 x 100 = 0,000454 gram

    PERHITUNGAN OSMOLARITAS

    • Lidocaine

    = m zat/BM x n x 1000

    = 24 / 234,34 x 1 x 1000

    = 102,45 mOsmol/L

    • Epinephrine Bitartrate

    = m zat/BM x n x 1000

    = 0,0024 / 183,304 x 1 x 1000

    = 0,013 mOsmol/L

    • Potassium Metabisulfite

    = m zat/BM x n x 1000

    = 0,144 / 188,177 x 1 x 1000

    = 0,428 mOsmol/L

    • EDTA Disodium

    = m zat/BM x n x 1000

    = 0,003 / 336,16 x 1 x 1000

    = 0,0089 mOsmol/L

    • Sodium Chloride

    = m zat/BM x n x 1000

    = 0,492 / 58,44 x 1 x 1000

    = 8,419 mOsmol/L

    • HP-B-CD
    = m zat/BM x n x 1000

    = 13,662 / 1135 x 1 x 1000

    = 12,037 mOsmol/L

    • Sodium Sacharine

    = m zat/BM x n x 1000

    = 0,108 / 205,17 x 1 x 1000

    = 0,526 mOsmol/L

    Total Osmolaritas = 123,8819

    Nilai Osmolaritas yang diperoleh berada dalam rentang <350 yang menandakan larutan bersifat
    hipotonis, sehingga diperlukan penambahan HCl

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