SBF3043 Animal and Plant Physiology

Instruction for Practical

Learning outcome
Conduct experiments scientifically to investigate a hypothesis.

Instructions
1. You will be given 2 practical manuals: one is related to animal physiology, and
another one is related to plant physiology.
2. It’s compulsory for you to conduct both experiments.
3. Setup the experiments based on the manual given.
4. You are required to record a video throughout the experiments, starting from the
setup process till the end of the observation stage.
5. You may perform the experiments between:
a. Week 5 – 7 for Experiment 1.
b. Week 10 – 12 for Experiment 2.

Video
1. The length of the video is 5 minutes maximum each.
2. You yourself have to be in the video explaining everything about the experiments.
3. Your video should include:
a. Title
b. Name and matric number
c. Objectives
d. Introduction
e. Hypotheses
f. Materials and Methods – you have to clearly show all the steps in the video
g. Results
h. Discussion
i. Conclusion
j. References
4. Deadline of the video (or the link to your video) submissions:
a. Friday Week 7 (3 December 2021) for Experiment 1.
b. Friday Week 12 (14 January 2021) for Experiment 2.
5. Please refer to the rubric below for more information on the marking scheme.
 Lab report rubric (video)
Item Excellent (4)
1 Objectives Shows a solid understanding of the
purpose of the experiment
2 Introduction Provides all necessary background
principles for the experiment
3 Hypothesis Generates an insightful hypothesis
4 Materials & Methodology All materials and methods used in
the experiment are clearly and
accurately described
5 Results/Finding/Observation Professional, clear and accurate
s representation of the data.
Tables/figures are labelled and titled
6 Discussion Results are discussed thoroughly
and analysed
7 Conclusion All important conclusions are clearly
made
8 References Several references. All with correct
format
TOTAL
Good (3)
Shows a considerable
understanding of the purpose of the
experiment
Missing some important background
principle for the experiment
Generates a valid hypothesis
Some materials and methods used
in the experiment are not clearly
and accurately described
Accurate representation of the data.
Tables/figures are labelled and titled
Some results are discussed and
analysed
Some of the important conclusions
are made
Several references. Some with
some correct format
(_____ / 32)
Fair (2)
Shows some understanding of the
purpose of the experiment
Missing many important
background principle for the
experiment
Generates a questionable
hypothesis
Most of the materials and
methods used in the experiment
are not accurately described
Accurate representation of the
data. Tables/figures are not
labelled and titled
Some results are discussed but
misleading
Some of the important
conclusions are made but
misleading
Several references with incorrect
format
Poor (1)
Shows very limited understanding of
the purpose of the experiment
No important background principle for
the experiment is stated
Has some difficulty generating a
hypothesis
Many materials and methods used in
the experiment are described
inaccurately or are not described at all
Data are inaccurate or not shown
Results are not discussed
Conclusions are not stated
No references at all
 Psychomotor performance rubric (Baharom et al., 2013).
Criteria
1 Successfully performs
experiments without
supervision.
2 Ability to organise,
performs experiments
safely and aware of priorities in
the laboratory.
3 Ability to show
engagement in conducting
experiment.
4 Ability to demonstrates
care and respect in
equipment set-up.
TOTAL
Advanced
(3 points)
Successfully completes
experiment procedures
independently. Shows
excellent understanding
of procedure and theory
taught.
Practices safely, can work
Independently and take
initiative as well as cooperating
effectively in a team.
Shows excellent performance with
unusual energy, is very focused,
shows confidence and full
commitment.
Always demonstrates
respect and care for
equipment.
Proficient
(2 points)
Successfully completes
experiment procedures with minimal
supervision.
Shows good understanding of
experiments procedure and theory
taught.
Practices most procedures safely
conforms to the lab
regulations with minimal supervision.
Shows good performance
with confident, energy and
commitment.
Good demonstration
of respect and care for
equipment.
(_____/12)
Functional
(1 points)
Successfully completes
experiment procedure with
moderate supervision. Show
minimal understanding of
experiments procedure and theory
taught.
Minor flaws in safety. Requires
constant
supervision.
Performs with some energy, focus
and
commitment.
Acceptable demonstration of
respect and care for equipment.
Developing
(0 points)
Cannot completes
tasks and standard
procedures. Show no
understanding of experiments
procedure and theory taught.
Fails to notice important
information and safety
factors in the workplace.
Performs with little energy, focus
and no
commitment needs support.
No demonstration of respect and
care for
equipment.
Experiment 1
Respiration

Rate of respiration

OBJECTIVE

Identify the effect of water temperature on the respiration rate of a fish

MATERIALS
Beaker (100 ml)
2 small fishes

PROCEDURES
1. Fill a beaker with 200 ml of tap water (normal temperature) and place a fish in the
beaker.
2. Observe and record the number of breaths every minute. You can determine the
number of breaths by counting the number of times the fish opens and closes its
mouth or the number of times the operculum contract. You have to do the observation
for 5 minutes.
3. Repeat step 1-2 using 200 ml of water from fridge (cold water).

RESULTS
Treatment Number of breaths in 5 minutes
Normal temperature
Cold water

DISCUSSION
1. How does water temperature affect the respiration rate of fish?
2. Beside temperature, concentration of oxygen is another factor that could affect the
rate of respiration in fish. Discuss this statement.