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    Expt 6 Data Sheet

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    Sweetzeil Tapia
    The document describes an experiment to isolate soluble ribonucleic acid (RNA) from yeast. [1] The procedure involves lysing yeast cells with alkali and heat, then centrifuging to separate insoluble residue from soluble RNA in the supernate. [2] Acid is added to precipitate the RNA, which is separated from the supernatant by centrifugation. [3] Ethanol and ether are used to wash and purify the isolated RNA.

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    Expt 6 Data Sheet

    Uploaded by

    Sweetzeil Tapia
    14 views3 pages
    The document describes an experiment to isolate soluble ribonucleic acid (RNA) from yeast. [1] The procedure involves lysing yeast cells with alkali and heat, then centrifuging to separate insoluble residue from soluble RNA in the supernate. [2] Acid is added to precipitate the RNA, which is separated from the supernatant by centrifugation. [3] Ethanol and ether are used to wash and purify the isolated RNA.

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    chemistry

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    The document describes an experiment to isolate soluble ribonucleic acid (RNA) from yeast. [1] The procedure involves lysing yeast cells with alkali and heat, then centrifuging to separate insoluble residue from soluble RNA in the supernate. [2] Acid is added to precipitate the RNA, which is separated from the supernatant by centrifugation. [3] Ethanol and ether are used to wash and purify the isolated RNA.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as docx, pdf, or txt
    14 views3 pages

    Expt 6 Data Sheet

    Uploaded by

    Sweetzeil Tapia
    The document describes an experiment to isolate soluble ribonucleic acid (RNA) from yeast. [1] The procedure involves lysing yeast cells with alkali and heat, then centrifuging to separate insoluble residue from soluble RNA in the supernate. [2] Acid is added to precipitate the RNA, which is separated from the supernatant by centrifugation. [3] Ethanol and ether are used to wash and purify the isolated RNA.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as docx, pdf, or txt
    of 3

    Group Members: Date Performed:

    Score /28
    Percentage %
    Group No. Section:

    Experiment 6
    ISOLATION OF SOLUBLE RIBONUCLEIC ACID

    OBJECTIVE

    _____________________________________________________________________

    _____________________________________________________________________

    _____________________________________________________________________

    DATA/OBSERVATIONS

    Describe the RNA obtained: (4 pts)


    a. color:
    b. texture: _______

    QUESTIONS

    1. Give the purpose of the following reagents in each step involved in the isolation
    procedure performed. (8 pts)

    a. alkali

    __________________________________________________
    __________________________________________________
    __________________________________________________

    b. acid

    __________________________________________________
    __________________________________________________
    __________________________________________________

    c. acidified alcohol __________________________________________________


    __________________________________________________
    __________________________________________________

    d. ether
    __________________________________________________
    __________________________________________________

    1
    __________________________________________________

    2. The following is an incomplete schematic diagram for the isolation procedure used.
    Label each step with the reagents used and products obtained. (16 pts)

    Yeast

    (a)_______________, heat, centrifuge

    residue 1 (discard) supernate 1

    A) _____________ (b) _________, pH, centrifuge

    residue 2 (discard) supernate 2

    B) ________________ evaporate, filter

    residue 3 (discard) supernate 3

    Cool to 40oC

    + c)________

    + conc. HCl

    residue 4 supernate 4

    alcohol + d) ___________

    residue 5 supernate 5

    C) ________________ D) _____________________

    Legend:

    2
    a,b,c,d : reagents added
    A,B,C,D : products obtained

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