Name _____________________________________________________________

DMD 3-A Date:Nov.

Year & Section: ___________ 19, 2021Grade:______________
____________

Laboratory Instructor’s Signature:___________________________________

Activity Report No. 16

Microbiota of Peridontal Pocket and Root Canal

1. Enumerate at least five (5) major periodontal pathogens and differentiate

them based on Grams stain reaction, oxygen requirement, morphological
arrangement, microscopic view.

Periodontal Gram Oxygen Morphologic Microscopic view

Pathogen stain requirement al (Picture)
reactio arrangemen
n t
1. Aerotolerant Can be
Porphyromon and can be short
as gingivalis stored in rod-
aerobic shaped, or
conditions in broth
but it will not culture,
coccobacill
grow in the
i
presence of
0.5 µm by
oxygen at
1- 2 µm in
levels higher size. On
than 6% solid
media,
colonies
are
generally
smooth,
shiny and
convex
2. Treponema Motile, slend
Treponema denticola, a er, helically
denticola periodontal shaped and
pathogen, flexible.
has recently
been shown
to exhibit
properties of
 a facultative
anaerobic
spirochete,
in contrast to
its previous
recognition
as an
obligate
anaerobic
bacterium
3. Fusobacteriu Pointed rods
Fusobacteriu m nucleatum that are
m nucleatum is an often in pairs
anaerobic arranged
Gram- end-to-end.
positive oral
commensal
bacterium.
4. Obligate Rod-shaped
Prevotella anaerobic bacterium is
Intermedia pathogenic also
bacterium classified as
a black
pigmented
bacteria
because of
its formation
of shiny and
smooth
colonies
which appear
either a grey,
light brown,
or black
color on
blood agar
plates.
5. Obligately Short rod-
Porphyromon anaerobic, shaped, or in
as non-motile broth
endodontalis culture,
coccobacilli
0.5 µm by 1-
2 µm in size.
On solid
 media,
colonies are
generally
smooth,
shiny and
convex.

2. Enumerate at least five (5) major pathogens found on an endodontically

infected tooth and differentiate them based on Grams stain reaction, oxygen
requirement, morphological arrangement, microscopic view.

Pathogens on Gram stain Oxygen Morphologica Microscopic

Endodontically reaction requirement l arrangement view
infected tooth (Picture)
1.

2.

3.

4.

5.