D.A.

V Public School
B.S.E.B Colony , New Punai Chak
Patna – 80002

Assistance for Biology Practical

(how to write practicals and maintain file)

Class - X

Provided by : Mrs. Kumari Bandana

Biology Teacher
 Dos for Practical File
📌 Prepare A practical file of bio (NOT COPY) with thread.
Click here to view image of Practical File
📌 Cover the file with white chart paper.
📌 Write Practicals on bio sheets (interleaf).
Click here to view image of bio sheet.
📌 Use pens for writing practicals:-
Black for heading
Blue for content
📌 DRAW only one diagram which you have observed (may be
under microscope)
📌 Always draw diagram with pencil.
📌 Prepare the file in a correct format:
1. leave first page totally blank .
2. Prepare acknowledgement , then index, then write practicals
on next pages.
3. Write the aim of experiment in index under experiment name .
Click here to view format of acknowledgement
📌 Try to keep the file simple.

Don'ts for Practical File

📌 Don't use colour pens , stickers, sketch pens or other

decorative items.
📌 Don't colour diagram.
📌 Don't draw or label diagram with pen, sketch pen .
Practicals for Class – X
1. Experimentally show that CO2 is given out during respiration.
2. Studying (a) binary fission in amoeba ,
(b) budding in yeast and hydra with the help of
prepared slides.

Click here to view Practical Contents

Format for Writing Practicals

📝 Experiment No.
(write in centre )

📝 Aim :- write the aim of experiment.

📝 Materials Required :- write name materials required

in experiment .(not in points , but in paragraph with
commas(,) in between them)

📝 Theory :- write a short description about the

experiment.

📝 Procedure :- write the steps carried out in doing the

experiment . (always write it in past tense)

📝 Observations :- write the observation of experiment

(during / after the experiment).

📝 Conclusion :- write the result of experiment.

📝 Precaution :- write the precaution that should be

taken during experiment .

📝 Caution :- write the caution that should be taken.

An Important Point :- Draw the related well labelled
diagram on plane side after writing procedure . It is
better to draw it on sheet left of observation.
Note : - Caution is optional
 A Sample Practical
Experiment No. 1
Aim :- To show experimentally that CO2 is given out during
respiration.

Materials Required :- A conical flask, a glass tube bent

twice at right angles (delivery tube), a beaker containing
water , a cork with a hole , a small test tube, freshly
prepared KOH solution, thread, vaseline , some germinating
seed, moist cotton.

Theory :-Respiration is a catabolic process which involves

the breakdown of food or complex organic molecules into
simpler products, with the release of energy. This process
can take place either in the presence of oxygen (aerobic
respiration) or in its absence (anaerobic respiration).
The overall reaction mechanism of aerobic respiration
involves the oxidation of carbohydrate and the subsequent
production of CO2, H2O and energy.
Aerobic Respiration :-
C6H12O6+6O2 → 6CO2 + 6H2O + Energy (38 ATP)

Procedure :-
1. Kept some wet and germinating seeds on moist cotton
in a conical flask.
2. Hanged a small test tube containing conc. 20%
solution of KOH in the flask by means of a thread .
3. Closed the mouth of a flask by a rubber cork having a
hole in the centre.
 4. Connected a bent glass tube to a flask ,through the hole
of cork and dipped the other end of the tube in the water of
beaker.
5.First we have taken initial reading as (h1) in the beaker of t
water.
6. Kept the whole setup for about 45 minutes in the bright
sunlight.
7.We have taken final reading as (h2) in the beaker of water.
Observations :-
• Water level rised up in the delivery tube.
• Recorded the change in water level in the delivery tube.
Conclusion :-
• The rising of the water level indicates that CO2 is
absorbed by KOH solution .
• This creates vacuum in the conical flask and hence
suction pull causes capillary action.

Precaution :-
1. All connection should be airtight .
2. Freshly prepared conc. solution of KOH should be
used.
3. Seeds should be wet, moist and germinating.
Diagram :-