Initial Reaction Rate 1/Initial Reaction 1/Substrate

z Concentration
(V) Rate (1/V) (1/S)
mmol/L mmol/L min L min/mmol L/mmol
1 0.2 5.000 1.000
2 0.22 4.545 0.500
3 0.3 3.333 0.333
5 0.45 2.222 0.200
7 0.41 2.439 0.143
10 0.5 2.000 0.100
15 0.4 2.500 0.067
20 0.33 3.030 0.050

From Equation
c = 1/Vmax 2.225
Vmax 0.449

m = km/vmax 3.039
Km = m*Vmax 1.366
 6.000

5.000 f(x) = 3.03867281573321 x + 2.224903446203

R² = 0.782638553434082

4.000

3.000
1/V

2.000

1.000

0.000
0.000 0.200 0.400 0.600 0.800 1.000 1.200
1/S
 Initial Reaction
Substrate Initial Reaction Rate Rate/Substrate
Concentration (S) (V) Concentration (V/S) 0.6
mmol/L mmol/L min 1/min
1 0.2 0.200 0.5
2 0.22 0.110
3 0.3 0.100 0.4 f(x) = − 1.21143558034183 x + 0.44994233
5 0.45 0.090 R² = 0.43578063334311

7 0.41 0.059 0.3

V
10 0.5 0.050
15 0.4 0.027
0.2
20 0.33 0.017
0.1
V = - Km(V/S) + Vmax
y = m*x + c
0
0.000 0.050 0.100 0.1
From Equation
V/S
C = Vmax 0.4499
m = Km 1.2114
f(x) = − 1.21143558034183 x + 0.449942339704455
R² = 0.43578063334311

0.050 0.100 0.150 0.200 0.250

V/S
 Substrate
Substrate Initial Reaction Rate Concentration/Initial
Concentration (S) (V) Reaction Rate (S/V) 70.000
mmol/L mmol/L min min
1 0.2 5.000 60.000
2 0.22 9.091 f(x) = 2.71753349145469 x − 0.1029198027
50.000
3 0.3 10.000 R² = 0.946183615776184

5 0.45 11.111 40.000

7 0.41 17.073

S/V
10 0.5 20.000 30.000
15 0.4 37.500
20 0.33 60.606 20.000

10.000
(S/V) = S*(1/Vmax) + (Km/Vmax)
y = m*x + c
0.000
0 5 10
From Equation S
m = 1/Vmax 2.718
Vmax 0.368
C = Km/Vmax 0.103
Km = Vmax*c 0.038
 3349145469 x − 0.102919802732182
83615776184

5 10 15 20 25
S