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SULIT
STPM Biology
964/1
4 Oktober 2017
1 hour 30 minutes

SEK MEN. KEB. TINGGI MELAKA

(Malacca High School. Estd. 1826)
Ke Arah Kecemerlangan Pendidikan
For examiner’s use
(Untuk kegunaan
TRIAL FIRST TERM pemeriksa
Section A :
FORM LOWER 6
Section B (Bahagian B)
16

17
BIOLOGY PAPER 1
Section C(bahagian C)
(1 hour 30 minutes)

Total
(Jumlah)

Instructions to candidates:
DO NOT OPEN THIS QUESTION PAPER UNTIL YOU ARE TOLD TO DO SO.

There are fifteen questions in Section A. For each question, four choices of answers are given.
Choose one correct answer and indicate it on questions paper. Answer all questions. Marks will not
be deducted for wrong answers.

Answer all questions in Section B. Write your answers in the spaces provided.

Answer any two questions in Section C. Answers may be written in either English or Bahasa Melayu

This question paper consists of 8 printed pages

___________________________________________________________________________
Prepared by Approved by
_______________________________ _______________________
(Puan Salbiyasota Abd Majid) (Tuan Hj Azmi bin Sakmis)
Biology Teacher (Form 6) Senior Assistance (Form 6)
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CONFIDENTIAL*

Section A [15 marks]

Answer all questions in this section

1 Match the properties of water with its importance to living organisms.

Property of water Importance to living organisms
i. High specific heat capacity (a). Terrestrial animals have relatively constant
ii. Low viscosity body temperature
iii. High surface tension (b). Allows mosquitoes to land on water
iv. Universal solvent (c) . Blood flows easily through narrow capillaries
(d). Blood plasma transports nutrients

A i(a) ii(c) iii(d) iv(b)

B i(a) ii(c) iii(b) iv(d)
C i(b) ii(a) iii(d) iv(c)
D i(c) ii(b) iii(a) iv(d)

2 Which statement is not true regarding the cellulose molecules?

A It is made up of beta-glucose monomers
B The long straight chain is linked by β-1,4 glycosidic bonds
C The side branches are linked by β -1,6 glycosidic bonds
D Parallel chains are cross-linked by hydrogen bonds.

3 The diagram show a molecule

Which substance might include this molecule

A cellulose C cholesterole
B glycerol D glucagon

4 Which of these molecules are linked to form a nucleotide containing uracil?

A 2, 3 and 5 C 2, 4 and 5
B 1, 3 and 5 D 1, 4 and 5
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5 The diagram shows the ultrastructure of an animal cell

Synthesizing Transporting Processing Secreting

proteins from proteins proteins digestive
amino acids enzymes

A 2 1 4 3

B 1 4 2 3

C 2 3 4 1

D 1 3 2 4

6 Which structure is found in all eukaryotic cells?

A Ribosome
B Centriole
C Chloroplast
D Mesosome

7 The table below shows three organelles and their functions ?

Organelle Function
P Chloroplast (a) Digestion of tissues
Q Lysosome (b) Reduction of coenzyme
R Mitochondrion (c) Destruction of organelles
(d) Oxidation of fatty acids
(e) Oxidative phosphorlyation
(f) Photophosphorylation

P Q R
A (b) ,(f) (a) ,(c) (d), (e)
B (a), (c) (b), (e) (d), (f)
C (d) , (e) (a), (f) (b), (c)
D (d) , (f) (a), (c) (b), (e)

8 Which of the following structures contain pseudo-stratified epithelium.

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i. Digestive tract
ii. Trachea
iii. Endothelium
iv. Skin

A ii only
B i and ii
C i, ii and iii
D ii, iii and iv

9. Which statement is not true involving the movement of substances in and out of cells?
A Charged ions diffuse through hydrophilic protein channels
B Small uncharged molecules diffuse through the phospholipid bilayer
C Facilitated diffusion using carrier proteins requires energy
D Endocytosis involves the bulk transport of materials into cells

10 Which is true of non competitive inhibitor ?

A Its mode of action is not reversible
B It bind directly to the enzyme at the active site
C Its binding to enzyme lowers the activation energy
D Its inhibitory effect can be reduced by increasing the substrate concentration

11 Which is not an advantage of using an immobilized enzyme system?

A Enzymes are trapped on the inert matrix, hence will not contaminate the end product .
B Cost of operation is much cheaper.
C Enzymes are more stable and are less likely to be denatured.
D Faster rate of reaction as the substrates has higher chances of entering enzymes active
site.

12 Which process does not occur during glycolysis ?

A Synthesis ATP C Reduction of NAD
B Hydrolysis ATP D Oxidation of NADH

13 Which statements describe the functions of anaerobic respiration?

i. to regenerate NAD+
ii. to prevent the increase of pH in cells by accumulation of pyruvate
iii. to produce CO2 to be used in the baking process
iv to continue energy production in cells in the absence of oxygen

A i and ii C ii and iii

B i and iv D iii and iv

14 Which is true about the light independent process in plants?

A Hatch-Slack pathway occurs only in CAM plants
B Krantz anatomy is only present in the leaves of C4 plants
C PEP carboxylase enzyme is only found in C4 plants
D Calvin cycle only occurs in C3 and C4 plants

15 In the diagram below, X is the compensation point of sun plants.

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Which statements are true?

i. P is the rate of photosynthesis
ii. Q is the rate of respiration
iii. R is represented by CO2 concentration
iv. On point X, all oxygen gas produced in the respiration is used for photosynthesis

A i and ii C ii and iii

B i and iii D iii and iv

Write your answers in the box below

1 6 11
2 7 12
3 8 13
4 9 14
5 10 15

Section B [ 15 marks ]

Answer all questions in this section

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16 The diagram below shows the lock and key hypothesis for enzyme activity

(a) Identify the molecules A, B and C. [3 marks]

A : _________________________________________________

B : _________________________________________________

C : _________________________________________________

(b) Briefly explain the lock and key hypothesis [2 marks]

_________________________________________________________________

____________________________________________________________________

( c) State one example of molecule B and from which vitamin it is derived from.
[1 mark]
__________________________________________________________________

(d) Explain the effect of the non-competitive inhibitor that attaches to site X
[ 1 mark]
__________________________________________________________________
__________________________________________________________________

17 The diagram below shows a technique used in cell biology. The homogenate used was
produced from fresh animal tissue.
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a) Name the technique shown in the diagram above? [1 mark]

_______________________________________________________________________

b) What is the purpose of the technique named in (a) ? [1 mark]

__________________________________________________________________

c) State one control step that has to be taken during this procedure . Explain. [2 marks]

__________________________________________________________________

___________________________________________________________________

d) Identify the cell component X, Y and Z. Which were isolated from the homogenate

[3 marks]
X : ____________________________________________________

Y: ____________________________________________________

Z: _____________________________________________________

e) State why an electron microscope has to be used to observed cell component Z

[1 mark]

______________________________________________________________________________

Section C [ 30 marks ]

Answer any two questions in this section

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18 (a) Lipids are used for energy storage and as a respiratory substrate.
(i) List three other roles of lipids in the human body. [3 marks]
(ii) Other than obesity, outline why a diet high in lipids might have a negative effect
on the health of an individual [3 marks]
(b Two examples of lipid molecules are triglycerides and phospholipids.
) Identify two differences and two similarities in the structures of [4 marks]
triglycerides and phospholipids.

(c) Explain how the composition of structures plasma membrane and

primary cell wall determines the permeability properties of these [5 marks]
structures.

19 (a) Outline the similarities and differences in the way that mitochondria and chloroplasts
generate a proton gradient and synthesis ATP. [6 marks]

(b) Explain how do the electron transport chain extract energy from hydrogen acceptors.
[9 marks]

20. a) Explain how light energy is converted to chemical energy of ATP. [5 marks]

b)Explain the differences between an action spectrum and an absorption spectrum in the
context of photosynthesis. [2 marks]

c) Using a labelled diagram, explain the main stages of Calvin cycle. [8 marks]

END OF QUESTION PAPER

MARKING SCHEME TRIAL P1 964/1 2017

Section A

1. B 2. C 3. D 4. D 5. C
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6. A 7. A 8. A 9. C 10. A
11. D 12. D 13. B 14. B 15. A

Section B

16 a) A: Prosthetic group(attach permanently to enzyme)

B : Coenzyme (attach temporarily to enzyme)
C: NADH + H+/ NADH
b) - only one type of substrate that have specific configuration can fit into the active site of a
specific of enzyme
- to form enzyme substrate complex
c) -NAD+ (nicotinamid adenine dinucleotide) from vitamin B6 (niacin)
d)- the substrate is prevented from attaching to the active site/ preventing formation of enzyme –
substrate complex / the rate of the enzyme catalysed reaction//V max is decreased.

17 a) -Ultracentrifugation
b) -to separate and isolate cell component
c) -The sample has to be kept in cold isotonic salt buffer solution/ the temperature must be kept
low
- prevents changes in the shape and volume of organelles in the solution / to slow down
metabolisme to prevent digestion of organelle and autolysis
d) X: Nucleus
Y: Mitochondrion
Z: Ribosomes
e) - to observe structures smaller than 2000nm.

Section C

18. (a) (i)  Membranes // phospholipid bilayer; 1

 Absorption of fat soluble vitamins; 1
 Electrical insulation / insulation of, neurons / nerve cells / axons // (thermal)
insulation; 1
 Protection of organs; 1
 (source of)(steroid) hormones / named steroid hormone / named group of steroid
hormones // testosterone, oestrogen, progesterone, aldosterone, glucocorticoids, 1
androgens; 1
 (source of) cholesterol / LDL / HDL;
 Water-proofing / skin suppleness / sebum // ear wax; 1
 Source of vitamin D; 1
 Buoyancy
(Any 3) 1
Max 3

(ii)  (leads to) increased, cholesterol / LDL; 1

 Cholesterol / fat / LDL, deposited, in arterial wall / under endothelium / epithelium / 1
lining;
 Increase risk of / lead to, atherosclerosis / artheriosclerosis / atheroma / plaque 1
formation; 1
 Narrowing / blocking, of artery lumen;
 Increased risk of / leads to angina / stroke / hypertension / high blood pressure / heart
attack / myocardial infarction / gallstones; 1
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(Any 3) Max 3

(b) Differences

Triglyceride Phospholipid

Consist of 3 fatty acids Consist of 2 fatty acids 1

Forming of 3 ester bonds Forming of 2 ester bonds 1
1
Absence of phosphate group Presence of phosphate group

Similarities 1
 (contain) glycerol; 1
 (contain) fatty acids;
1
 (contain) ester bonds;
 (contain elements) C, H and O; 1
2 max for differences
2 max for similarities Max 4

(c) Plasma membrane

 Partially permeable / selectively permeable; 1
 Phospholipid (bilayer) permeable to, lipid-soluble molecules / oxygen; 1
 Impermeable to, water-soluble molecules /ions;
 Transport proteins provide permeability to, ions / polar molecules; 1
 Channel / pore / carrier, proteins 1
1
Primary cell wall
 Permeable;
 Cellulose fibres; 1
1
 Spaces / gaps / holes / pores, between, fibres;
1
(Any 3 + 2 / 2 + 3)
Max 5

19 a)

ATP Synthesis

Similarities 1
1
1. Both need high energy electrons 1
2. Both need stalked particles/ ATP synthetase 1
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3. Both need proton pumps 1

4. Both involve a series of carrier/pumps in electron transport chain 1
5. Both need protons which are pumped into a intermembrane space
6. Both are involved in creating an electrochemical/chemiosmotic/ 1
H-ion/H+/proton gradient (any 3)
7. Both involve redox reactions

Differences 1

1. Cristae/ (folded) inner membrane carries stalked particles in mitochondria 1

whereas thylakoid membrane in chloroplasts carries stalked particles
2. Water formed during ATP synthesis in mitochondria whereas water broken 1
down (in non-cyclic photophosphorylation) in chloroplasts
3. Protons derived from glucose breakdown in mitochondria whereas in 1
chloroplast protons derived from water/photolysis which produces H ions
4. High energy electrons are derived from glucose/reduced NAD/FAD in
mitochondria whereas high energy electrons are derived from chlorophyll in (any 3)
chloroplasts MAX 6

19 b)
1. The ETC consists of 3 protein complexes; NADH dehydrogenase/FMN FeS 1
complex, cytochrome bf complex and cytochrome oxidase complex. ETC also
consists of two mobile electron carriers; ubiquinone Q and cytochrome c

2. The order of the carrier in ETC is FMN FeS comples, ubiquinone Q, 1

cytochrome bf complex, cytochrome c and cytochrome oxidase

3. NADH and H+ pass two hydrogen (2 protons H+, and 2 electron) to FMN-FeS 1
complex

4. Electrons are passed down the ETC 1

5. Each member in the chain is reduced when accepting electrons 1

6. It becomes oxidized when passes electrons to the next carrier down the 1
chain

7. The last acceptor of electron is oxygen which reacts with H+ to form water 1

8. The energy that is release during the transport of electrons is used to pump 1
hydrogen ions from the matrix into the intermembrane space

9. As the membrane is impermeable to H ions, a high H+ concentration is built 1

up in the intermembrane space
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10. ATP synthase allows H+ to flow down its concentration gradient 1

11. The energy released is used to synthesise ATP 1

12. The process of regenerating ATP from ADP and Pi using energy derived from 1
the redox reaction of an ETC is called oxidative phosphorylation

(any 9)
20. a)

1. Light behaves as though it consists of particles called photons that carry a fixed quantity of 1
energy (depending on the wavelength.
2. When accessory pigment molecules in a photosystem (in chloroplast) absorb photons of light, 1
the energy is transferred from one accessory pigment molecule to another and is eventually
passed to a special chlorophyll a molecule in the reaction centre of the photosystem.
3. Electrons from the reaction centre are excited to a higher energy level and displaced. The
excited electrons are passed on through a series of electron carriers called primary electron 1
acceptors.
4. Energy released as the electrons move from higher energy level to a lower level as they pass
through the various electron acceptors is used to create a proton gradient; hydrogen ions are
pumped actively from the stroma, across the thylakoid membrane, into the thylakoid space. 1
5. The diffusion of protons (H+) out of the thylakoid space, down its concentration gradient provides
the energy for the synthesis of ATP from ADP and P i in the presence of ATP synthase
(chemiosmotic synthesis of ATP).
1

Any 5
5 marks
20 b)

1. The action spectrum shows the relationships, usually plotted as a graph, between the 1
wavelengths of light and the rate of photosynthesis for a photosynthetic pigment,
2. whereas the absorption spectrum shows the percentage of absorption by a photosynthetic 1
pigment at different wavelength of light.

2 marks
20 c) D= 1m
L = 2m

3marks
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1
1

1
1

Any 2
1. Carbon dioxide fixation
1
 Ribulose biphosphate (RuBP) is fixed with carbon dioxide to form an unstable six-carbon 1
sugar compound.
 The process is catalysed by the enzyme ribulose biphosphate carboxylase/ RuBisCo
 The unstable six-carbon compound immediately splits into two molecules of a three-carbon 1
compound called glycerate 3-phosphate (GP) or phosphoglyceric acid (PGA).
Any 1

1
2. Reduction phase
1
 Phosphorilation of PGA by ATP to form a "bis- phosphate” and Glycerate 3-phosphate (PGA)
is reduced (by NADPH) to glyceraldehydes 3-phosphate (PGAL) or triose phosphate .
 NADPH and ATP are the products of the light-dependent reaction. 1

Any 1
3. Regeneration of carbon dioxide acceptor / RuBP
1
 5/6 of GALP (3C) molecules enter a series of reaction driven by ATP, which results in the 1
regeneration of RuBP (5C).
 This is an important stage to ensure supply of RuBP is continuous for the fixation of carbon 1
dioxide.
Any 1
4. Product synthesis phase
 The remaining 1/6 of the PGAL molecules are used to make glucose, which can be converted to Total=
starch, cellulose, acid amino/protein and lipids. 8m
 The carbon and oxygen atoms of sugar (C 6H1206) come from carbon dioxide while the hydrogen
atoms come from NADPH.
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