Satvika Bejawar

Week 2 Lab Protocol fermentation

General Biology Labaratory
Week 2 Protocol: Fermentation

Research Question: How does the difference in PH level affect the rate of fermentation?

Hypothesis: Our hypothesis is that PH 6 is the optimal PH for fermentation. If we add a PH of 6

to the experiment, then the reaction will produce more CO2 and expel the water droplets faster.

Ingredients Table:

Condition Yeast Substrate (5mL) pH Buffer

Negative Control 10 mL DI water Glucose 10% 5mL of pH 7

pH 2 10 mL Glucose 10% 5 mL

pH 6 10 mL Glucose 10% 5 mL

pH 8 10 mL Glucose 10% 5 mL

Protocol:
1. Obtain 4 beakers and label them pH 2, 6, and 8, and one for the negative control
2. In each beaker put 10mL of yeast, DI water in control, 5mL glucose 10%, and 5mL pH
buffer
3. Allow each beaker to settle for 5 minutes, and swirl periodically
4. Then draw 3 mL of each solution into syringes, 3 syringes for each pH buffer except for
the negative control
5. Invert each syringe and draw 1 mL of air into each syringe
6. Add a small length of tubing, and place the pointed pipette into the 50 mL di water
7. Try to allow a small droplet of water to move into the pipette by capillary action
8. When the water droplet reaches the end of the pipette, put a pipette on each syringe
9. Then record the volume of CO2 produced until it reaches the 0mL mark
10. Record at 1 minute intervals for 10 minutes