Cobe Spectra Apheresis System

INTRODUCTION:

The COBE Spectra System will perform all types of apheresis procedures. One of its
main procedures is to collect single donor platelets. Because of the unique abilities of this
device, the procedures are individualized by the control of several algorithms. Prior to the
beginning of the run, a platelet yield is predicted using the entered platelet count. Based upon
this information, changes can be made in the operating parameters to optimize the collection.
Leuko-poor thrombocytes are produced which can be stored for 5 days. The collect
concentration monitor, an on-line device, provides information on the use of thrombocyte
concentration and yield, without the use of probes.

I. PRINCIPLE:
The system draws whole blood from a donor or patient, adds anticoagulant, separates
the blood components, collects or removes specific components and returns uncollected
components to the donor or patient. In therapeutic plasma exchange and red blood cell
exchange procedures, appropriate replacement fluid is continuously returned.
The Cobe Spectra System Includes:

 Four pumps
 Five valves
 Optical sensors to monitor density in the plasma/collect line or red blood cell spillover.
 Display panel providing instructions and system messages.
 Centrifuge chamber with interlocking door and cover.
 Return flow controller that enables single-needle therapeutic plasma exchange and
platelet collection.

Procedures on the COBE Spectra system deliver:

 Dual-stage or single-stage technology.

 Separation of components based on specific gravity of cells.
 Configurable procedure parameters for procedure consistency.
 Automated procedures with operator control.
 Programmable fluid balance and automated procedure endpoint calculations.
 Low total blood volume (TBV) applications.

The COBE Spectra system sets and maintains the interface by defining the pump flow rates and
centrifuge speed based on donor or patient data for a specific procedure. The wide range of
continuous inlet flow rate settings allows the system to manage vascular access and
accommodate small patients. The two-way communications display and an associated keyboard
enhance the automation.
II. MATERIAL & REAGENTS:

 Disposable kit
 ACDA
 Normal Saline
 Fistula Needle
 Physio Ball Hand exerciser
 Surgical tape
 Emergency kit

III. Operating Procedure

 Check power supply.

 Clean all sensor and valves with gauze piece.
 Switch on power button.
 Hang / load disposable kit.
 Close access and return needle clamps and rollers.
 Attach fluids with disposable.
 Open access and return lines rollers.
 Start Priming the disposable kit.
 Enter donor values (Height, weight, Hematocrit, and platelet count)
 Adjust Platelet conc yield, collection volume and inlet flow.
 Prime access and return connection lines (fistula).
 Close access saline line roller and test ACD A ratio and perform alarm test.
 Prepare the donor.
 Attach the donor access and return connection lines.
 Open the clamps of return and access connection lines.
 Start draw.
 After diverting saline end, close the return saline line roller and press clear.
 Inventory recovery option show press 1 for recovery.
 At the end of collection lock/seal the product the product bag.
 Clamp access needle and Open access saline roller.
 Start and complete Rinse back.
 Disconnect the donor.
 Apply bandage on puncture site.
 Remove the tubing set and discard.

Reference:
Machine manual