Table 1: Central composite rotatable matrix for feather degradation and keratinase production by Bacillus aerius

NSMk2.

Run Factors Experimental results Predicted results

A: pH B: C: Feather Feather Keratinase Feather Keratinase

Temperatur concentration degradation activity degradation activity
e (%, w/v) (%) (U/ml) (%) (U/ml)
(°C)
1 7.5 35 1.375 82.90 127.45 80.59 125.58

2 7.5 35 1.375 82.96 127.58 80.59 125.58

3 8.5 25 0.75 55.29 111.12 55.26 112.26

4 8.5 25 2.0 19.12 109.13 17.96 115.49

5 7.5 35 0.32 60.12 124.23 58.11 125.03

6 5.81 35 1.375 35.11 75.24 41.65 75.34

7 6.5 45 2.0 34.32 90.34 34.11 91.90

8 7.5 51.81 1.375 35.23 90.64 32.14 92.44

9 7.5 35 1.375 80.12 126.13 80.59 125.58

10 8.5 45 2.0 39.24 106.67 37.11 106.97

11 7.5 35 1.375 82.67 126.43 80.59 125.58

12 6.5 45 0.75 40.43 108.67 38.35 105.01

13 7.5 35 1.375 83.09 127.63 80.59 125.58

14 7.5 18.18 1.375 0.87 80.12 8.55 74.51

15 6.5 25 2.0 25.25 70.12 15.02 71.85

16 8.5 45 0.75 54.23 112.56 61.22 113.53

17 7.5 35 2.42 19.11 121.12 25.71 116.73

18 9.18 35 1.375 65.32 123.12 63.36 119.21

19 7.5 35 1.375 83.01 126.61 80.59 125.58

20 6.5 25 0.75 30.56 72.76 29.44 75.16

Table 2: Variance analysis of response surface quadratic model for feather degradation and keratinase production by
Bacillus aerius NSMk2.

Source Feather degradation (%) Keratinase production (U/ml)

Sum of Df F- p-value Sum of Df F-value p-value
squares value squares
Model 11914.48 9 18.60 <0.0001 7530.41 9 46.03 <0.0001
A-pH 568.68 1 7.99 0.0179 2322.99 1 127.79 <0.0001
B- 671.83 1 9.44 0.0118 388.10 1 21.35 0.0009
Temperature
C-Feather 1267.17 1 17.81 0.0018 83.21 1 4.58 0.0581
concentration
AB 0.0018 1 0.00 0.9961 408.27 1 22.46 0.0008
AC 197.41 1 2.77 0.1268 21.42 1 1.18 0.3032
BC 51.92 1 0.729 0.4130 47.97 1 2.64 0.1353
6
A2 1420.88 1 19.97 0.0012 1443.69 1 79.42 <0.0001
2
B 6539.20 1 91.89 <0.0001 3194.26 1 175.71 <0.0001
2
C 2695.84 1 37.88 0.0001 39.87 1 2.19 0.1694
Residual 711.61 10 181.79 10
Lack of fit 337.04 5 0.899 0.5447 139.49 5 3.30 0.1081
8
Pure error 374.54 5 42.29 5
Cor total 12626.09 19 7712.19 19