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The Cell Membrane: AP Biology
The Cell Membrane: AP Biology
AP Biology
Overview
§ Cell membrane separates living cell from
nonliving surroundings
u thin barrier = 8nm thick
§ Controls traffic in & out of the cell
u selectively permeable
u allows some substances to cross more easily
than others
§ hydrophobic vs hydrophilic
§ Made of phospholipids, proteins & other
macromolecules
AP Biology
Phosphate
Phospholipids
§ Fatty acid tails
u hydrophobic
§ Phosphate group head
Fatty acid
u hydrophilic
§ Arranged as a bilayer
Aaaah,
one of those
structure–function
examples
AP Biology
Phospholipid bilayer
polar
hydrophilic
heads
nonpolar
hydrophobic
tails
polar
hydrophilic
heads
AP Biology
More than lipids…
§ In 1972, S.J. Singer & G. Nicolson
proposed that membrane proteins are
inserted into the phospholipid bilayer
AP Biology
Membrane is a collage of proteins & other molecules
embedded in the fluid matrix of the lipid bilayer
Glycolipid
Phospholipids
Cholesterol
Transmembrane
proteins
Peripheral
protein
Cytoplasm Filaments of
cytoskeleton
AP Biology
Membrane fat composition varies
§ Fat composition affects flexibility
u membrane must be fluid & flexible
§ about as fluid as thick salad oil
u % unsaturated fatty acids in phospholipids
§ keep membrane less viscous
§ cold-adapted organisms, like winter wheat
w increase % in autumn
u cholesterol in membrane
AP Biology
Membrane Proteins
§ Proteins determine membrane’s specific functions
u cell membrane & organelle membranes each have
unique collections of proteins
§ Membrane proteins:
u peripheral proteins
§ loosely bound to surface of membrane
§ cell surface identity marker (antigens)
u integral proteins
§ penetrate lipid bilayer, usually across whole membrane
§ transmembrane protein
§ transport proteins
w channels, permeases (pumps)
AP Biology
Why are
proteins the perfect
molecule to build structures
in the cell membrane?
AP Biology 2007-2008
Classes of amino acids
What do these amino acids have in common?
I like the
polar ones
the best!
AP Biology
polar & hydrophilic
Proteins domains anchor molecule
Polar areas
§ Within membrane of protein
u nonpolar amino acids
§ hydrophobic
§ anchors protein
into membrane
§ On outer surfaces of
membrane
u polar amino acids
§ hydrophilic
§ extend into
extracellular fluid &
into cytosol Nonpolar areas of protein
AP Biology
H+
Examples Retinal
chromophore
NH2
water channel
in bacteria
Porin monomer
β-pleated sheets
Bacterial Nonpolar
outer (hydrophobic) COOH
membrane α-helices in the
cell membrane H+
Cytoplasm
Plasma
membrane
Inside
Transporter Enzyme Cell surface
activity receptor
AP Biology
Any Questions??
AP Biology
Movement across the
Cell Membrane
AP Biology
Diffusion
§ 2nd Law of Thermodynamics
governs biological systems
u universe tends towards disorder (entropy)
§ Diffusion
AP Biology
u movement from high → low concentration
Diffusion
§ Move from HIGH to LOW concentration
u “passive transport”
u no energy needed
movement of water
IN OUT
food waste
carbohydrates OUT ammonia
sugars, proteins salts
amino acids CO2
IN
lipids H 2O
salts, O2, H2O products
AP Biology
cell needs materials in & products or waste out
Diffusion through phospholipid bilayer
§ What molecules can get through directly?
u fats & other lipids
lipid
§ What molecules can
inside cell NOT get through
NH3 salt directly?
u polar molecules
§ H2O
u ions
§ salts, ammonia
sugar aa H 2O
outside cell u large molecules
§ starches, proteins
AP Biology
Channels through cell membrane
§ Membrane becomes semi-permeable
with protein channels
u specific channels allow specific material
across cell membrane
NH
AP Biology
3
salt outside cell
Facilitated Diffusion
§ Diffusion through protein channels
u channels move specific molecules across
cell membrane
facilitated = with help
u no energy needed
open channel = fast transport
high
low
AP Biology “The Bouncer”
Active Transport
§ Cells may need to move molecules against
concentration gradient
u shape change transports solute from
one side of membrane to other
u protein “pump”
ATP
high
AP Biology “The Doorman”
Active transport
§ Many models & mechanisms
ATP ATP
antiport symport
AP Biology
Getting through cell membrane
§ Passive Transport
u Simple diffusion
§ diffusion of nonpolar, hydrophobic molecules
w lipids
w high → low concentration gradient
u Facilitated transport
§ diffusion of polar, hydrophilic molecules
§ through a protein channel
w high → low concentration gradient
§ Active transport
u diffusion against concentration gradient
§ low → high
u uses a protein pump ATP
u requires ATP
AP Biology
Transport summary
simple
diffusion
facilitated
diffusion
active ATP
transport
AP Biology
How about large molecules?
§ Moving large molecules into & out of cell
u through vesicles & vacuoles
u endocytosis
AP Biology exocytosis
Endocytosis
fuse with
phagocytosis lysosome for
digestion
pinocytosis non-specific
process
triggered by
receptor-mediated molecular
endocytosis signal
AP Biology
The Special Case of Water
AP Biology 2007-2008
Osmosis is diffusion of water
§ Water is very important to life,
so we talk about water separately
§ Diffusion of water from
high concentration of water to
low concentration of water
u across a
semi-permeable
membrane
AP Biology
Concentration of water
§ Direction of osmosis is determined by
comparing total solute concentrations
u Hypertonic - more solute, less water
u Hypotonic - less solute, more water
u Isotonic - equal solute, equal water
water
hypotonic hypertonic
AP Biology net movement of water
Managing water balance
§ Cell survival depends on balancing
water uptake & loss
AP Biology balanced
Managing water balance
§ Hypotonic
u a cell in fresh water
§ example: Paramecium
§ problem: gains water,
swells & can burst
w water continually enters
Paramecium cell
§ solution: contractile vacuole
ATP w pumps water out of cell
w ATP
u plant cells
§ turgid
AP Biology freshwater
Water regulation
§ Contractile vacuole in Paramecium
ATP
AP Biology
Managing water balance
§ Hypertonic
u a cell in salt water
§ example: shellfish
§ problem: lose water & die
§ solution: take up water or
pump out salt
u plant cells
§ plasmolysis = wilt
AP Biology saltwater
1991 | 2003
Aquaporins
§ Water moves rapidly into & out of cells
u evidence that there were water channels
.05 M .03 M
AP Biology