Certificate of Analysis

Certificate of Analysis ID: 1104260500_VM807526_EN

Producer and client: Merck KGaA, Frankfurter Str. 250, 64293 Darmstadt, Germany

Test laboratory: Merck KGaA Qualitätskontrolle für mikrobiologische Produkte

Frankfurter Str. 250, 64293 Darmstadt, Germany

Sample identification: Chromocult®

Coliform Agar acc. ISO 9308-1

Ordering number: 1.10426.0500

Lot number: VM807526

Accreditation: The test laboratory of Merck KGaA is accredited by

the German accreditation authority DAkkS as
registered test laboratory D-PL-15185-01-00
according to DIN EN ISO/IEC 17025 for the
performance testing of media for microbiology
according to DIN EN ISO 11133:2014.

Test method: Performance testing of solid culture media:

Quantitative method (membrane filtration)
Qualitative method (membrane filtration)

Date of analysis: 2017/10/06

Date of release: 2017/11/01

Minimum shelf life: 2022/09/27

Composition (g/l):
Enzymatic digest of casein 1.0; Yeast extract 2.0; Sodium chloride 5.0;
Sodium dihydrogenphosphate dihydrate 2.2; di-Sodium hydrogen-
phosphate 2.7; Tryptophane 1.0; Sodium pyruvate 1.0; Tergitol® 7
0.15; Sorbitol 1.0; Agar-agar 10.0; 6-chloro-3-indoxyl-beta-
Dgalactopyranoside 0.2; Isopropyl-beta-D-thiogalactopyranoside 0.1;
5-bromo-4-chloro-3-indoxyl-D-glucuronic acid 0.1

Preparation & sterilization: Dissolve 26.5 g in 1 liter of purified water. Heat in boiling water and
agitate frequently until completely dissolved. Do not autoclave, do not
overheat!

Application: For the simultaneous detection of coliform bacteria and E. coli in

drinking water, waters with low bacterial background flora and
processed food samples.

Storage: Store at +15 °C to +25 °C, dry and tightly closed. Do not use clumped
or discolored medium. Protect from UV light (including sun light).

The reported results refer exclusively to the specified medium, see Certificate of Analysis ID.

Merck KGaA · Frankfurter Straße 250, 64293 Darmstadt, Germany: +49 6151 72-2440
EMD Millipore Corp. · 290 Concord Road, Billerica, MA 01821, USA +1-978-715-4321 1104260500_VM807526_EN
ATCC is a registered trademark of ATCC, Manassas, VA, USA. Lit. No. TN105406EN00 Page 1/2
Certificate of Analysis
Physical parameters Specification Lot value
Appearance (clarity): slightly turbid to turbid turbid
Appearance (color): yellowish to yellowish-brown yellowish
pH-value (25 °C): 6.6 – 7.0 6.8

Microbiological Performance

Quantitative method for solid media (membrane filtration)

Test strain Specification Reference Test Recovery Typical reaction

CFU CFU rate
Escherichia coli ≥ 70 % 232 222 96 % passes test
ATCC® 25922 dark-blue to violet
[WDCM 00013] colonies
Escherichia coli ≥ 70 % 197 179 91 % passes test
ATCC® 8739 dark-blue to violet
[WDCM 00012] colonies
Enterobacter aerogenes ≥ 70 % 198 170 86 % passes test
ATCC® 13048 pink to red
[WDCM 00175] colonies
Citrobacter freundii ≥ 70 % 136 136 100 % passes test
ATCC® 43864 pink to red
[WDCM 00006] colonies

Qualitative method (membrane filtration)

Test strain Specification Growth Typical reaction

Enterococcus faecalis partial inhibition partial inhibition -

ATCC® 19433
[WDCM 00009]
Pseudomonas aeruginosa growth no limit, good passes test
ATCC® 10145 colorless colonies colorless colonies
[WDCM 00024]
Incubation: 21 ± 3 hours at 36 ± 2 °C aerobic
Reference medium: Tryptic Soy Agar without membrane filter
Filter type: Mixed Cellulose Esters (MCE), Pore size 0,45 µm

A recovery rate of 70 % is equivalent to a productivity rate of 0.7.

The indicated colony counts result from the sum of a triple determination.
All test methods listed are according to DIN EN ISO 11133:2014.

Signature:

Dr. Stefanie Fischer

Head of Quality Control Microbiology

Merck KGaA · Frankfurter Straße 250, 64293 Darmstadt, Germany: +49 6151 72-2440
EMD Millipore Corp. · 290 Concord Road, Billerica, MA 01821, USA +1-978-715-4321 1104260500_VM807526_EN
ATCC is a registered trademark of ATCC, Manassas, VA, USA. Lit. No. TN105406EN00 Page 2/2