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Cell Structure and Functions - 2021-22 - Stud
Cell Structure and Functions - 2021-22 - Stud
Cell Structure and Functions - 2021-22 - Stud
Functions
1. Cellular organization
2. Cell size limitations
3. Cells can reproduce
4. Cells possess genetic program
5. Cells acquire and utilize energy
6. Cells carry out chemical reactions
* CELL AS BASIC UNIT OF LIFE
II. Basic Properties of Cells
1. Cellular organization
Prokaryotes (Simple)
• bacteria
• archaebacteria (unique features, and features
resemble bacteria and eukaryotes)
Eukaryotes (Complex)
• plants, animals, fungi, algae and protozoa
* Difference:
Eukaryotes have membrane-bound organelles
* CELL AS BASIC UNIT OF LIFE
2. Cell size
- Limitations on cell size
• Requirement for an adequate ratio of
surface area / volume
• Diffusion rates of molecules
• Maintain adequate concentrations of
reactants and enzymes
* CELL AS BASIC UNIT OF LIFE
Limitations on cell size
• Requirement for an adequate surface area /
volume ratio
I. Microscopy
• The science of the interpretive use and applications
of microscopes.
e.g. light microscope and electron microscope
Advantage
➢ high resolution (0.2 nm)
Disadvantages
➢ specimen must be dead because it is viewed in a
vacuum
➢ specimen preparation may change or damage the
structure, time-consuming and requires expert
training.
➢ expensive to buy and run
Images observed using TEM
* THE STUDY OF CELLS
2. Electron microscope
1. Homogenization
2. Differential centrifugation
* THE STUDY OF CELLS
II. Cell fractionation
1. Homogenization
• cells are homogenized by mechanical disruption
in a blender to break the cell wall and plasma
membrane.
• carried out in a cold, isotonic, buffered solution
Supernatant
Pellet
* THE STUDY OF CELLS
II. Cell fractionation
2. Differential centrifugation
• skeleton of cytoplasm
• made of filamentous protein network
• present in plant and animal cells
• a dynamic structure
• Functions
➢ maintains cell shape, enables cellular motion
➢ play important roles in intracellular transport and
cell division
* THE CELL AND ORGANELLES
II. Interior structure of cell – Cytoskeleton
Three types of fibers:
a) Microfilaments
b) Microtubules
c) Intermediate filaments
* THE CELL AND ORGANELLES
II. Interior structure of cell – Cytoskeleton
a) Microfilaments
• Functions
➢muscle contraction and movements of certain
cells
➢provide support (more in muscle cells and
amoeboid)
• major portion of cytoskeleton, two chains twisted
around one another.
• long fibers but thin, diameter = 6-7 nm
• thinnest filaments, flexible and strong
• each subunit is made of actin (protein).
* THE CELL AND ORGANELLES
II. Interior structure of cell – Cytoskeleton
b) Microtubules
Functions:–
• involved in many cellular processes:-
- transport vesicle
- provide cell shape and support
- separate chromosomes during cell division
* THE CELL AND ORGANELLES
II. Interior structure of cell – Cytoskeleton
b) Microtubules
• straight, hollow rods (d = 25nm)
• made of β and α-tubulin (protein)
• part of the cilia and flagella of eukaryotic cells
• form spontaneously around centrosomes
(microtubule organizing centre)
• unstable, easily assembled and disassembled
* THE CELL AND ORGANELLES
* THE CELL AND ORGANELLES
II. Interior structure of cell – Cytoskeleton
c) Intermediate filaments
• Functions
➢preventing excessive stretch of cells shape
➢fix organelles position
• most durable and stable
• diameter varies, from 8 to 12nm
• made of vimentin (protein)
example: keratin of the skin cell
* THE CELL AND ORGANELLES
• Exterior structure:-
➢ Cell wall (plants and bacteria)
➢ Plasma membrane
➢ Cilia and flagella
• Interior structure:-
➢ Cytoplasm
➢ Cytoskeleton (in eukaryotes)
➢ Organelles (in eukaryotes) – 3 groups:-
▪ Endomembrane system
▪ Mitochondria and chloroplast
▪ Ribosomes and nucleus
• Eukaryotic cell (plant cell)
• Eukaryotic cell (animal cell)
* THE CELL AND ORGANELLES
II. Interior structure of cell – Organelles
Class I: Endomembrane system
• a group of membranes and organelles in eukaryotic
cells that work together to modify, package, and
transport lipids and protein
Smooth ER
• found in a variety of cell types (both animal and
plant) and it serves different functions in each
• cisternae are tubes-like, connected with RER,
smooth surface
Function
• convert light energy of the sun into sugars that can
be used by cells.
• process is called photosynthesis by chlorophyll
molecules in each chloroplast.
* THE CELL AND ORGANELLES
ii. Chloroplast
• double membrane separated by intermembrane space
• stroma contain ribosomes, DNA and photosynthetic
enzymes (stroma - dark reaction site)
• internal membrane is called lamella which consist of
thylakoids that stacks up to form grana.
• each chloroplast has about 50 grana - each granum
(singular) has about 50 thylakoids.
• thylakoid membrane (surface) is the light reaction site.
• photosynthetic pigments (chlorophyll a, b, carotenoid),
electron transport chain (ETC) are embedded within the
thylakoid membrane.
* THE CELL AND ORGANELLES
ii. Chloroplast
* THE CELL AND ORGANELLES
iii. Nucleus
• largest organelle (6μm), first to be discovered
• enclosed by a nuclear envelope - double
membrane perforated by nuclear pores (100nm).
• nuclear membrane is impermeable to large
molecules
• nuclear pores - channel that allows free
movement of small molecules and ions
• outer membrane is continuous with membrane of
RER
• the space between the membranes i.e.
perinuclear space is continuous with the RER
lumen
* THE CELL AND ORGANELLES
iii. Nucleus
* THE CELL AND ORGANELLES
iii. Nucleus
• nucleoplasm is the semi-fluid substance in the
nucleus.
• nucleus contains mainly the genetic material in
the form of chromatin or chromosomes
• contain nucleolus, function - synthesis ribosomes
* THE CELL AND ORGANELLES
iii. Nucleus
The End