2

Libyan International Medical University

Faculty of Pharmacy

[Clonin Model]
Student Name : Nouralhodah Almukasaby
Student no: 3103
Tutor Name : Khadeja Najeb
Block No.6
Week No.2
Date of Submission : 2021/12/8

Academic Year
 2020-2021
2

Table of Contant
Inroduction………………………………………………………..3
[1]-Draw The Digram of high perfomance liquid chromatography…..4
[2]-Define DNA sequence ,Cheomosome libraries and Genomic
library………………………………………………………………….…4
[3]-Compere between immediate and delayed hypersenstivty………..5
[4]-List factors increase and decrease intracellular free cholesterol
concentration……………………………………………………………..5
[5]-Describe metabolic fate of cholesterol inside the body……………6
[6]-Compere between Carcinoma and sarcoma………………………..6
Summary…………………………………………………….…….8
Reference…………………………………………………………..9

Table of figure

[1]- Figure1: HPLC Shape……………………………………………………4

[2]- Figure2: Compere between immediate and delayed hypersenstivty…….5
[3]- Figure3: metabolic fate of cholesterol inside the body………………….6

Table of table

[1]- Table1: Compere between Carcinoma and sarcoma………………..……7

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Introduction
This problem to talk about Draw The Digram of high perfomance liquid
chromatography and Define DNA sequence ,Cheomosome libraries and Genomic
library and Compere between immediate and delayed hypersenstivty and List factors
increase and decrease intracellular free cholesterol concentration and Describe
metabolic fate of cholesterol inside the body and Compere between Carcinoma and
sarcoma and finally Summary.
2
[1]-Draw The Digram of high perfomance liquid chromatography:

Figure1: HPLC Shape

(6b5a048b2988da554ea4e3a7ab10a3dccdee6fd7 @ Whatishplc.Com, n.d.)

[2]-Define DNA sequence, Chromosome libraries and Genomic

library:
DNA sequence A clone from a genomic library, or any other clone, can be analyzed
to determine the nucleotide sequence of the DNA insert, as well as to determine the
distribution and location of restriction sites. Its nucleotide sequence is the most
detailed information one can obtain about a DNA fragment. The information is useful,
for example, in computer database analyses for comparing sequences from different
genomes, which can tell us how closely related two organisms are, or for identifying
gene sequences and the regulatory sequences—like promoters, silencers, and
enhancers—that control gene expression.(Hartog et al., 1967)
A library consisting of a collection of cloned DNA fragments derived from one
chromosome is called a chromosome library.(Hartog et al., 1967)
A genomic library is a collection of clones that contains at least one copy of every
DNA sequence in an organism’s genome.(Hartog et al., 1967)
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[3]-Compere between immediate and delayed hypersenstivty:

Figure2: Compere between immediate and delayed hypersenstivty

(Kumar, 2016)

[4]-List factors increase and decrease intracellular free cholesterol

concentration:
Cholesterol balance in tissues: Many factors will determine the cholesterol balance at
the cellular level.
 Increasing:

 increase is due to uptake of cholesterol-containing lipoproteins by receptors

 the LDL receptor or the scavenger receptor.
 uptake of free cholesterol from cholesterol-rich lipoproteins to the cell
 hydrolysis of cholesteryl esters by the enzyme cholesteryl ester hydrolase
 Phosphatases dephosphorylate (fed state) and activate HMG-CoA activity;
therefore increasing cholesterol.
 Increased synthesis of cholesterol.(Murray & Davis, 2003)

 decreasing:

 Decrease is due to efflux of cholesterol from the membrane to HDL

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 promoted by LCAT (lecithin: cholesterol acyltransferase)
 esterification of cholesterol by ACAT (acy l- CoA: cholesterol
acyltransferase)
 utilization of cholesterol for synthesis of other steroids, such as hormones, or
bile acids in the liver.
 inhibation synthesis of cholesterol.
 down-regulates synthesis of the LDL receptor.(Murray & Davis, 2003)

[5]-Describe metabolic fate of cholesterol inside the body:

Figure3: metabolic fate of cholesterol inside the body

(TRUTH @ Cholesterol2006.Narod.Ru, n.d.)

[6]-Compere between Carcinoma and sarcoma:

Carcinoma Sarcoma
Define Cancers that grow in soft the Cancers that grow in the epithelial
epithelial cells Less common
connective or supportive tissues, more
common
Example Adenocarcinoma ,squamous Osteosarcoma (bone) and
cell carcinoma ,anaplastic chondrosarcoma (cartilage)
carcinoma

Aga More above 45 More in yong adult

Rate of growth Slower than sarcoma Very rapid
Frequency common Rare
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Spread Early by blood sarcoma Early by lymphatic's
Prognosis Less worse More worse
Blood supply less Rich with hemorrhage and necrosis
And blood vessels
Histology Cells arranged in groups Cells arranged individual
Premalignant phase May have a pre-malignant Not believed to have a pre-malignant
phase phase
Striking Age group Generally, below fifty Generally , above fifty
Form and consist Usually hard Soft or fleshy
Commend side Any where lung liver bone Lung
brain
Radio sensitivity More sensitivity Less sensitivity

Table1: Compere between Carcinoma and sarcoma

(430ebeb0bd8d088e52e6212b1e11da6a861f5d83 @ Veterinarysciencehub.Com, n.d.)
(What-Is-the-Difference-between-Carcinoma-and-Sarcoma @ Www.Quora.Com,
n.d.)
(6c3479d7fe3c20e3dfd358dc22d876b4c1098704 @ Www.Facebook.Com, n.d.)
(DeLong, 2013)
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Summary

I understood Draw The Digram of high perfomance liquid chromatography and

Define DNA sequence ,Cheomosome libraries and Genomic library and Compere
between immediate and delayed hypersenstivty and List factors increase and decrease
intracellular free cholesterol concentration and Describe metabolic fate of cholesterol
inside the body and Compere between Carcinoma and sarcoma and finally Summary.
2
Reference430ebeb0bd8d088e52e6212b1e11da6a861f5d83 @
veterinarysciencehub.com. (n.d.).
http://veterinarysciencehub.com/differentiate-the-carcinoma-from-the-sarcoma-
to-better-understand/
6b5a048b2988da554ea4e3a7ab10a3dccdee6fd7 @ whatishplc.com. (n.d.).
https://whatishplc.com/hplc/hplc-instrumentation__trashed/
6c3479d7fe3c20e3dfd358dc22d876b4c1098704 @ www.facebook.com. (n.d.).
https://www.facebook.com/pathology.made.easy/photos/difference-between-
carcinoma-and-sarcoma-pathology-mbbs-neetpg/1487558788070637/
DeLong, L. (2013). Basic pathology. In General and Oral Pathology for the Dental
Hygienist (pp. 29–44). https://doi.org/10.1136/jcp.47.1.95-d
Hartog, F., Kim, S., Woo, M., Steele, J. L., Murnane, R. J., Willett, J. B., & F. Keifer
GEffenberger. (1967). 済無 No Title No Title No Title. Angewandte Chemie
International Edition, 6(11), 951–952., 3(2), 121–161.
Kumar, S. (2016). Essentials of Microbiology. In Essentials of Microbiology.
https://doi.org/10.5005/jp/books/12697
Murray, R. K., & Davis, J. C. (2003). Biochemistry (Lippincotts).
TRUTH @ cholesterol2006.narod.ru. (n.d.).
http://cholesterol2006.narod.ru/TRUTH.html
What-is-the-difference-between-carcinoma-and-sarcoma @ www.quora.com. (n.d.).
https://www.quora.com/What-is-the-difference-between-carcinoma-and-sarcoma