Gram Stain Procedure

Gram Stain:
Gran stain is a technique used in labs to distinguish gram-positive eubacteria
from gram-negative eubacteria.
Materials Used:
 Crystal violet
 Iodine
 Alcohol
 Safranin
 Water
 Tweezer
 Medical gloves
 Heat source
 Eye dropper
 Gram-positive bacteria (in agar solution)
 Gram-negative bacteria (in blood agar solution)
 Microscope
 Slides
The Procedure:
 We started the procedure by heating the tweezer in order to become
sterilized.
 Then we put a drop of water onto a slide.
 We put both gram-positive and gram-negative bacteria onto a slide by
using a tweezer then heated the tweezer to kill any remaining bacteria on
the tweezer to prevent harm, and damage to other people.
 We dropped Crystal violet on the slide by using eye dropper, then washed
the slide with water after 30 seconds.
 We put iodine on the slide then washed the slide after 1 minute.
 We put Alcohol on the slide and washed it after 10 seconds.
 We put Safranin on the slide which had a brown-reddish color, then
washed it after 1 minute.
 Then we put the slide under a microscope to observe it closely.
 Result:
After completing the procedure and looking at the slide under a light-
microscope, we observed that most of the bacteria we had were gram-negative
and their color were pink but we also saw some purple colored spherical
bacteria under the microscope which was the gram-positive bacteria.

Enos Salar
11-A