Fight AMR

NG-Test® CARBA 5
KPC, OXA-48-like, VIM, IMP, NDM Carbapenemases
Detection & Characterisation
CARBA

15 Rapid
min • Results in 15 minutes
{
• From bacterial culture
From direct blood culture* C
• Minimal hands on time
K
O
Accurate V
N
I
• Excellent correlation with PCR
• Numerous studies available

User friendly S
• Minimal training needs
• No equipment needed
• No maintenance costs
• Stable at room temperature

DE I N
MA
In use Worldwide in Microbiology labs
and National Reference Centers FR
A NCE

Rapid, mobile, connected diagnostics www.ngbiotech.com

*Using NG-Test Blood Culture Prep kit
 Fight AMR

Product Specimen Format Packaging Storage Shelf-life Cat. Ref.

NG-Test® CARBA 5 Culture Casse�e 20 tests 4-30°C 24 months NGB-CAR-S23-002
NG-Test® Blood Culture Prep Culture Vials 20 tests 20-30°C 24 months NGP-BCU-S73-002

Performance Characteris�cs WGS

CARBA Posi�ve Nega�ve Total
Detec�on limit
Posi�ve 119 0 119
The detec�on limits were determined using purified recombi- NG-Test® Nega�ve 0 28 28
nant enzymes: CARBA 5
KPC 600pg/mL Total 119 28 147
OXA 300pg/mL Sensi�vity : 100% CI 95% = 96.9% - 100%
VIM 300pg/mL Specificity : 100% CI 95% = 87.9% - 100%
C IMP 200pg/mL
K NDM 150pg/mL Variants* detected by NG-Test® CARBA 5:
O
V
Clinical Evalua�on Type NDM: NDM-1-2-3-4-5-6-7-8-9-11-19.
I
N Type KPC: KPC-1-2 -3 -4 -5 -6 -7 -12- 14 -23-28-39
NG-Test® CARBA 5 was evaluated at the NRC (AMR French
Referent Center, Kremlin-Bicêtre Hospital, Paris, France). Type IMP: IMP-1-2-4-5-6-7-8-10-11-13-14-15-16-18-19-22-26-29
147 strains were blind-tested with Whole Genome -31-37-39-46-47-56-58-61-63-71-79.
Sequencing (WGS)-characterized beta-lactamase content. Type VIM:VIM-1-2-4-5-6-19-23-26-27-31-39-46-51-52-54-56-58-59.
Two rares D179Y KPC variants (KPC-31 and KPC-33) were Type OXA-48-like: OXA-48 -58-162-181-204-232-244-245-370-436
S assessed as negative samples as they cannot be -484 -515-517-519-535-793.
considered as carbapenemases (carbapenem hydrolytic
activity impared).
Non-carbapenemases (cross-reactivity) : OXA-163 and OXA-405
Interpreta�on (OXA-48-like extended spectrum oxacillinases with very weak
carbapenemase activity).
Note: the above list may not be exhaustive
NG-Test ® CARBA 5 C
K
C
K
C
K
C
K *Studies & data on request.
O O O O
V V V V
I I I I
N N N N
These tests were developed in collabora�on with the CEA.
Negative VIM Positive Invalid Invalid The French Alterna�ve Energies and Atomic Energy Commission
NOTE: Mul�ple lines or one line on K, O, V, I, N posi�on must be (CEA) is a key player in research, development and innova�on.
considered as a posi�ve result

IDENTIFICATION PROCESS FROM BACTERIAL CULTURE OR DIRECT BLOOD CULTURE

+
Stool / Urine / Biological samples Selec�ve medium** 18-24H

CARBA

NG-Test CARBA 5
®
C

15 min
K
O
V

-
I
N

Posi�ve blood culture NG-Test blood culture Prep S

1-5 days 10 min

**Validated on: Luria Broth (LB) and LB agar, Trypticase soja agar (TSA), Mueller Hinton (MH) agar, UriSelect™ 4, Columbia agar + 5 % horse blood, ChromID® ESBL agar,
ChromID® CARBA SMART, Drigalski (DRIG) agar, CHROMagar™ mSuperCARBA™, TSA + 5 % sheep blood, Mac Conkey, HardyCHROM™ CRE agar.

Opera�ng procedure from bacterial culture

15
min
r
ffe
Bu
5 drops 100 µL 100 µL

culture
colonies
S

1 2 3 4 5

For professional in vitro diagnostic use only

NG Biotech
Z.A. Courbouton, secteur 1- 35480 Guipry, France
Phone: +33 (0)2 23 30 17 83 Fax +33 (0)9 71 70 53 10 info@ngbiotech.com

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