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    Aapap

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    | sensors (wort Article High-Throughput Continuous-Flow Separation in a Micro Free-Flow Electrophoresis Glass Chip Based on Laser Microfabrication Aodong Zhang '*, Jian Xu +, Xiaolong Li, Zijie Lin **, Yunpeng Song», Xin Li '*, Zhenhua Wang '** and Ya Cheng ‘24 "Engineering Research Center for Nanophotonics and Advanced Instroment, School of Physics nd Hletronic Science, Fast China Normal University, Shanghai 20024, China: ‘S2210920n@0stu een eden (AZ); ahwangophy ecnueds.cn (Z:M});yachengosiom.accn (.C) 2 State Key Laboratory of Precision Spectroscopy Schoo of Physics and Electronic Science, ast China Normal University, Shanghai 20024, China; 1174700030s.cems dem (5 S2i7os200d6rstu ecu edu.en (ZL) S2I9092107Tastu eu.ed.en (YS) > XXL The Extreme Optoslecromechanics Laboratory, School of Physics and Elestonic Science, ‘East China Normal Universi, Shanghai 200241, China 4 Slate Key Laboratory of High Feld Laser Physics, Sharh Institue of Optics and Fine Mechanics, Chinese ‘Academy of Sciences, Sangha 201800, China (Correspondence: posphy eens ca cn (Xj xiphy- conden (XL) Nel peromance ening spurten snd purfcaton of get gud saps ns md ane However te ace abrcaton af iE cevee hgh toughpu nd igh sty eae fut or conventional metas To ade he deg and fabs gh ough pt uFPE chip ae proposed ning lanrnited chem eng of gfe eecoede Fegraton rt beequnt lowmprstae bonding, Te art desg othe hlght a fhe Sepuntion chanber and elecode cana combined witha high eeu solatonllons iano Ure etcen emovel uf elas gneted buble lng the deep eee hans daring ect hala ow arparaton The neducuon of mirocannelanape ker cohanees the abi cree sol ton witha separation puny of 979% at vlage of 250 fom he ature Coinsoue- Flow Spain ‘Assdere Eo: Sinan Bor sample solution of fluorescein sodium and rhodamine 6 solution is demonstrate. Accept 24 January 202 Keywords: femtosecond laser microfabrication glass microluidieslaser-assisted chemical etching: Paso 1 February 22 micro fre-flow electrophoresis; continuous-flow separation Publishers Noes MDPL stays staimsinpubishelmapssndistts: 4 Inteoduction Micro free-flow electrophoresis (JIFFE) is a versatile technique for high-efficiency [OmO continuous separation and purification of liquid analytes with an electric-field-controlled microfluidic environment [1-6]. In FFE, the flow direction of liquid streams in a separa- CCopyigh:©202by theauhors tion microchannel or micro-chamber is perpendicular to the direction ofthe electric field, ceniee MDPL Basel. Swizeand Which enables the different displacements of the pre-separated samples to realize spatial ms open acess ars microscale separation depending on their electrical mobilities surrounded by the back- lr thems antcos ground solution under the electric field. Due to its remarkably continuous-flow capability Aion Crete Commons Al of online processing, this technique has shown broad prospects in chemical, biological, soutien CC BY nse and medical applications such as the high-performance purification of various fine chem- sseommonoriiersesbyiiO). jcale, proteins, and biomolecules, Sensors 222, 2, 1124, tps: ong/10 39022000124 ‘wove mdpicomfournallsentrs Sensors 200222, 1124 aot ‘To date, some representative methods to prepare .FFE chips have been developed [7-21]. For polymeric materials such as PDMS and ABS plastics, soft-lithography [8] or ‘three-

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