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    Dls

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    dls guide

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    Dls

    Uploaded by

    manigandan
    68 views4 pages
    dls guide

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    dls

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    dls guide

    Copyright:

    © All Rights Reserved
    Download as PDF or read online from Scribd
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    Download as pdf
    68 views4 pages

    Dls

    Uploaded by

    manigandan
    dls guide

    Copyright:

    © All Rights Reserved
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    Download as pdf
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    90% Ls instruments < ymamic Light Seattering (OLS, ‘DLS Data Analysis: The CORENN Metros DLS Miroviscometry ounces Scat Alte Sanaa Prue ASeses ates coats Lanting Atak Experimental Guidelines - DLS Sample Preparation 1) Sample ‘The messurement principle of DunamiLioht Satin (OLS) s based onthe ‘rowan motion of particles This rests the ange samples at canbe measured LS aiows measurement of calla, sch as + So particles, petymers and protains dispersed na solvent + Emusons LS does nt allow measurement of + ry powers these canbe measured only after homogenous esprsonin the sever 1) Solvent ‘Samples use for DLS shouldbe general persed in a gud ahase ter production or synthesis are purietion prepared ina resere a, in mest cases ‘amples should be lady persed form Inthe ce of ry semples (pots “event addon ie necesary peer to measutment. The moss common svn a + Detenzed oF MiiQ water + Toluene + Metanol + anol + Geert In order to choose 2 solvent. we man requitements should be ta4en into account Fray the solvent shoud perse the pats wel In some cases, tight be necessary to use dispersing agents such as steric one surfactants Ensure at the {cosen solvent along with the dispersing ager, ils suit cella tity over ‘he properties ofthe samples, eg the solvent shoud not solve the partes: ‘eteen reftactve indices shoul be thearget This wil ys the best signal st _9von sample conearvation the sample cannot be concerts and the signals net suffer arelable measvement the user should aden set equpnes with 3 ‘mote power ase. ae sue thatthe solvert used is of igh put ad does nat contain age molar mass Impurtes fog. dus. In he case of smal weal seataring partes, the absence of larpemoar mass mpurites sof great imaortance as thai seating signa couls shadow” the signa of te patel being measured, Large oar mas speces can be and containers sul asa be rns with Pgh purity solvent before bing sed forthe sample preparation For samples obtained ina surpencon form, flraton might be ured ae welt remus Lids arge molar mass specs Inthi a0, however, ar yrtheszedaoareaRe= mie also be removed, meaning tat the overall natie of he samp might be aored, ‘Choose ter wth an appropriate pre size (o ensue that parts itanded for ‘measurement are ntremaved, Note that the sample concentration might change uring the vain, due wo the adoration of purtees ont the iter Another possibly ta exclude agglomerates iste et them sediment aspy genie a Good Laboratory practic incles workin very clean contin daly in lean 00m, 3 aminar ow hood oF 9 wel-veniated fue hoea-Te ik f dus contamination willbe minimized by closing solvents, pistes, vis, and sales Sly. Adetonaly ‘wearing gloves is advisable nt only due tothe possni contamination of samples but so duet satery reasons As fr sample concentration, he lower the concen atin, ‘he higher the probable inuence tom arg molar ase impures Il) Concentration Measurable sample concentrations manly depend on the spcifeDLS measuring technique sdoptes Excare¥eiy encanvated amples shoul be ted with ine ame sven used to ruspend your prices. Overy ited sample can be ancentrates by caiaugation, removal othe slvert and subsequent -

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