COLEGIO DE MUNTINLUPA

POSADAS AVE., SUCAT, MUNTINLUPA CITY

CHEMISTRY FOR ENGINEERS LABORATORY

(NASC2011)

EXPERIMENT 6
DISSOLVED OXYGEN LEVELS IN NATURAL WATER
Preliminary Data Sheet

Section ME1B
:

Surname, Given Name, M.I. : MUNDO, Izzi

PALER, James Dominic
PINPIN, John Bernard
PINUELA, Jayvee
RADAM, Alnel
REYES, Thaddeus

Objectives:
 To determine the dissolved oxygen concentration of a natural water sample
 To learn the chemical reactions involved in fixing and analyzing a water
sample for dissolved oxygen using the Winkler method

Data:

Water source: Tap water

Sample Analysis

Sample volume 200 mL

Buret reading, initial 0 mL

Buret reading, final 11.2 mL

Volume Na2S2O3, mL 11.2 mL

Molar concentration of Na2S2O3 0.02512 mol/L

Moles of Na2S2O3 dispensed, mol 2.81 x 10-4

Moles I3- reduced, mol 1.41 x 10-4

Moles O2, mol 7.03 x 10-5

Mass O2, mg 2.251

 COLEGIO DE MUNTINLUPA
POSADAS AVE., SUCAT, MUNTINLUPA CITY

CHEMISTRY FOR ENGINEERS LABORATORY

(NASC2011)

Dissolved oxygen, ppm 11.25

Calculations:

A. Volume of Na2S2O3 dispensed (mL)

(11.2 – 0) mL = 11.2 mL

B. Moles of Na2S2O3 (mol)

Volume of Na2S2O3 x (mol/L Na2S2O3)

0.0112 L x (0.02512 mol/L Na2S2O3) = 2.81344 x 10-4 mol Na2S2O3

C. Moles of I3-

¿
Moles of S2O3 x 1 mol I 3− 2 mol S 2O 3−¿ ¿ ¿
¿
2.81344 x 10-4 moles of S2O3 x 1 mol I 3− 2 mol S 2O 3−¿ ¿ ¿ = 1.40672 x 10-4
mol I3-

D. Moles of O2

2mol Mn (OH ) 3 1 mol O 2

Moles of I3- x x
1 mol I 3−¿ ¿ 4 mol Mn (OH ) 3
2mol Mn (OH ) 3 1 mol O 2
1.40672 x 10-4 mol I3- x x = 7.0336 x 10-5 mol
1 mol I 3−¿ ¿ 4 mol Mn (OH ) 3
O2

E. Moles of O2

2mol Mn (OH ) 3 1 mol O 2

Moles of mol I3- x x
1 mol I 3−¿ ¿ 4 mol Mn (OH ) 3
¿
Volume of (L) S2O32- x mol S 2 O3 2− L S 2 O 32−¿ ¿ ¿ x

1 mol I 3− ¿ ¿ 2mol Mn (OH ) 3 1 mol O 2

2 mol S 2O 3 2−¿ ¿ x 1 mol I 3−¿ ¿ x 4 mol Mn (OH ) 3

F. Mass of O2

32 g 1000 mg
7.0336 x 10-5 mol O2 x x = 2.250752 mg O2
1mol O 2 1g

G. Dissolved oxygen (ppm O2)

 COLEGIO DE MUNTINLUPA
POSADAS AVE., SUCAT, MUNTINLUPA CITY

CHEMISTRY FOR ENGINEERS LABORATORY

(NASC2011)

2.250752mg O2
= 11.25376 ppm O2
0.2 mL

Guide Questions:

1. Is there a relationship between the temperature and the dissolved oxygen

concentration? If so, describe their relation.

Yes, there is a relationship between the temperature and the dissolved

oxygen concentration, dissolved oxygen concentration is inversely
proportionate with the temperature because when the solubility of water
decreases, the temperature increases and vice versa. This indicates that
deeper, cooler water requires less dissolved oxygen to achieve 100% air
saturation than warmer surface water.

2. What is the importance of knowing the amount of dissolved oxygen in water

quality?

One of the most primary indicators of water quality is dissolved oxygen (DO).
It is necessary for fish and other aquatic species to survive because too much
or too little dissolved oxygen can harm aquatic life and degrade water quality.
The aerating action of winds causes oxygen to dissolve in surface water.
Aquatic plant photosynthesis releases oxygen into the water as a byproduct.

3. What is the standard acceptable range of dissolved oxygen (DO) set by the
government agencies for water quality?

According to World Health Organization, depending on the temperature

requirements for specific aquatic species, the criterion values range from 5 to
9.5 mg l-1 for various life stages of species, i.e. a minimum dissolved oxygen
content of 5-6 mg l-1 for warm-water biota and 6.5-9.5 mg l-1 for cold-water
biota. Higher oxygen concentrations are also important in the early stages of
life. On the other hand, according to DENR, most fish cannot survive for
lengthy periods of time at levels below 3 milligrams per liter (mg O2/L), while
levels over 5 mg O2/L are considered ideal. Hypoxia occurs when oxygen
levels fall below 1 mg/L, and anoxia occurs when oxygen is completely
absent (officially known as anaerobic).
COLEGIO DE MUNTINLUPA
POSADAS AVE., SUCAT, MUNTINLUPA CITY

CHEMISTRY FOR ENGINEERS LABORATORY

(NASC2011)