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BIOLOGY – Assignment
Chapter : Biotechnology : Principles and Processes & Biotechnology and its Applications
1. Restriction endonucleases 9. The first restriction endonuclease reported was
1) Are enzymes that process pre-RNA’s. 1) Hind II 2) EcoRI
2) Are enzymes that degrade DNA. 3) Hind III 4) BamHI
3) Protect bacterial cells from viral infection.
10. Which one of the following is used as vector for
4) None of these cloning genes into higher organisms?
2. How many varieties of rice has been estimated to 1) Baculovirus
be present in India? 2) Salmonella typhimurium
1) 2,000 2) 20,000 3) Rhizopus nigricans 4) Retrovirus
3) 200,000 4) 2,000,000
11. Which one of the following palindromic base
3. RNA interference (RNAi) technique has been
devised to protect the plants from nematode is sequences in DNA can be easily cut at about the
silenced by ______ produced by the host plant. middle by some particular restriction enzyme?
1) dsDNA 2) ssDNA 1) 5'.............CGTTCG.............3'
3) dsRNA 4) Target proteins 3'.............ATGGTA.............5'
2) 5'.............GATATG.............3'
4. Which of the following is a critically important tool
used in experiments involving DNA 3'.............CTACTA.............5'
hybridization? 3) 5'.............GAATTC.............3'
1) DNA sequencing machines 3'.............CTTAAG.............5'
2) Ligases 4) 5'.............CACGTA.............3'
3) DNA probes 4) Vectors 3'.............CTCAGT.............5'
12. Natural genetic engineers of plants is
5. There is a restriction endonuclease called EcoRI.
What does co. part in it stand for? 1) Yeast
1) Colon 2) Coelom 2) Agrobacterium tumefaciens
3) Coenzyme 4) Coli 3) E. coli 4) Mycoplasma
13. A kind of biotechnology involving manipulation of
6. Select the option that is not a part of cell DNA is
transformation
1) DNA replication 2) Genetic engineering
1) Preparation of competent cells
3) Denaturation 4) Renaturation
2) Incubation of competent cells with foreign DNA
on ice 14. Which of the following is a plasmid?
3) Heat shock at 42°C 1) pBR 322 2) Bam H I
4) Not putting the DNA treated cells after heat 3) Sal I 4) Eco RI
shock back in ice 15. Match the following
7. Vectors include Column I Column II
1) Bacterial and plant plasmids A Ti plasmid (i) Separation and
2) Viruses purification of products
3) Artificial chromosomes B Biolistics (ii) Large scale production
4) All of the above of recombinant
proteins
8. Which of the following are known as specific
molecular scissors? C Bioreactors (iii) Microparticles of gold
coated with DNA
1) Ligase 2) Helicase
3) Restriction endonuclease D Downstream (iv) Agrobacterium
4) DNA polymerase processing tumefaciens

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1) A – (iii), B – (ii), C – (i), D – (iv)
2) A – (iv), B – (ii), C – (iii), D – (i)
3) A – (iv), B – (iii), C – (ii), D – (i)
4) A – (iv), B – (i), C – (ii), D – (iii)
16. Common bacteria used in genetic engineering is
1) E. coli 2) Diplococcus
3) Rhizobium 4) Spirillum
17. Genetic engineered bacteria is used for
production of
1) Insulin 2) Glucagon
3) Penicillin 4) All of these
18. Gel electrophoresis is used for
1) Cutting of DNA into fragments
2) Separation of DNA fragments according to their
size
3) Construction of recombinant DNA by joining
with cloning vectors
4) Isolation of DNA molecule
19. _______ is a procedure through which a piece of
DNA is introduced in a host bacterium
1) Transduction 2) Transformation
3) Conjugation 4) R.D.T
20. Ti-plasmid used in genetic engineering has
been modified by
1) Adding tumour forming genes.
2) Deleting tumour forming genes.
3) Adding genes for endonucleases.
4) Deleting genes for endonucleases.
21. The term “competent” refers to
1) Increasing the competition between cells
2) Making cells impermeable for DNA
3) Increasing the efficiency with which DNA enters
the bacterium through pores in its cell wall
4) Making cells permeable for divalent cations
22. Eli lily company (USA) developed the first
commercial recombinant product. It is named as
1) Human growth hormones
2) Tissue plasminogen activator
3) Humulin
4) Anti-hemophilic human factor
23. Genetically modified organism have been useful
in many ways, except
1) Enhanced nutritional value of food
2) Decreased efficiency of mineral usage
3) Crops more by plants tolerant to abiotic stress
4) Reduction in post harvest loss
24. A genetically engineered microorganism used
successfully in bioremediation of oil spills in
ocean is a species of
1) Trichoderma 2) Xanthomonas
3) Pseudomonas 4) Bacillus thuringiensis
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2 Biology Assignment
25. DNA or RNA segment tagged with radioactive
molecules is called
1) Vector 2) Probe
3) Clone 4) Plasmid
26. Crystals of Bt toxin produced by some bacteria
do not kill the bacteria themselves because
1) Bacteria are resistant to the toxin.
2) Toxin is immature.
3) Toxin is inactive.
4) Bacteria enclose the toxin in special sac.
27. The main technique involved in agricultural
biotechnology is called
1) Tissue culture 2) Transformation
3) Plant breeding 4) DNA replication
28. Cloning a gene involves
1) Restriction endonucleases and ligase.
2) Plasmids and bacteriophage lambda.
3) Yeast artificial chromosomes and
complementary base pairing.
4) All of the above
29. A cDNA clone contains
1) Mostly cytosine
2) A copy of the DNA identical to the nuclear
gene.
3) A copy of noncoding DNA
4) A DNA copy of mRNA
30. The linking of antibiotic resistance gene with the
plasmid vector became possible with the enzyme
1) DNA polymerase
2) DNA ligase
3) Restriction endonuclease
4) Polynucleotide kinase
31. Choose the incorrect statement w.r.t restriction
endonuclease
1) Each restriction endonuclease recognises a
specific palindromic nucleotide sequence in the
DNA
2) Palindrome in DNA is a sequence of base pairs
that reads same on the two strands when
orientation of reading is kept the same
3) Restriction enzymes cut the strand of DNA a
little away from the centre of palindrome sites,
but between the same two bases on the
opposite strands
4) Palindromic sequence read the same on the
two strands in 53´ direction, but not in 3
5´ direction
32. When cut by the same restriction enzyme,
generally the resultant DNA fragments have
the same kind of _______ and these can be
joined together (end to end) using ______.
1) Blunt ends, restriction endonuclease
2) Sticky ends, alkaline phosphatase
3) Flush ends, polynucleotide kinase
4) Sticky ends, DNA ligase
EXCEL ACADAMICS - Bangalore 3 Biology Assignment
33. The technique used for separation of different 40. A method in which recombinant DNAs are
sized fragments of DNA molecules is called incubated with host cells on ice, followed by
1) Elution placing them briefly at 42°C and then putting
back on ice is called
2) Gel electrophoresis
1) Heat shock method
3) Western blotting 4) Northern blotting
2) Transfection
34. The separated DNA fragments can be 3) Biolistic
visualised only after staining the DNA with 4) Gene gun
_______ followed by exposure to _______
41. In isolation of DNA, RNA and proteins are
1) Ethidium bromide, visual light
removed by using appropriate enzymes. The
2) Gram stain, visual light purified DNA ultimately precipitates out after the
3) Ethidium bromide, ultraviolet radiation addition of
4) KMnO 4, visual light 1) Ethidium bromide 2) DNase enzyme
3) Chilled ethanol 4) Heatshock treatment
35. The separated bands of DNA are cut from the
agarose gel and extracted from the gel piece, 42. Which coloured bands are visible on agarose
this process is called gel after staining DNA fragments?
1) Elution 2) Transduction 1) Blue black
3) Transformation 4) Electrophoresis 2) Brownish red
3) Bright orange coloured
36. Which of the following is true w.r.t origin of
replication (ori) site of cloning vector? 4) Deep red band
1) Sequence from where replication starts and
43. For transferring DNA to the host cell _______
any piece of DNA can be made to replicate
within the host cells if it has an ori site method increases the porosity of the
membrane by applying high electric potential
2) Helps in identifying and eliminating non-
transformants 1) Particle gun
3) Responsible for controlling the copy number 2) Electroporation
of the linked DNA 3) Biolistic
4) Both 1 & 3 4) Chemical mediated method
37. Which of the following method is involved with
44. Which of the following characteristics are not
selection of recombinants from non-
applicable to a plasmid?
recombinants?
1) Biolistics 1) Replication dependent upon host
chromosome
2) Insertional inactivation
2) Double stranded
3) Gel electrophoresis
4) Down streaming processing 3) Circular
4) Lacks histones
38. Which of the following antibiotic resistance
genes are present in pBR322 as selectable 45. Bacterial cells are made competent by treating
marker? them with divalent cations of
1) ampR and tetR 2) Bam H I and Hind II
1) Lithium 2) Copper
3) Lac Z gene 4) All of these
3) Calcium 4) Sulphur
39. Choose incorrect statement w.r.t. insertional
inactivation of lac Z selectable marker
46. The recombinant vector molecule cannot be
1) The presence of a chromogenic substrate
gives blue coloured colonies if the plasmid in created unless
the bacteria does not have an insert 1) The vector is cut with EcoRI and source DNA
2) Presence of insert results into insertional with Hind II
inactivation of the -galactosidase and colonies 2) The vector is cut with Hind II and source
do not produce any colour DNA with EcoRI
3) Recombinant bacteria are identified as blue 3) The vector is digested with exonuclease and
coloured colonies source DNA with EcoRI
4) A recombinant DNA is inserted within the 4) The vector and DNA are both digested with
coding sequence of an enzyme, - EcoRI
galactosidase

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EXCEL ACADAMICS - Bangalore 4 Biology Assignment
47. Propelling microscopically small titanium or gold 54. In genetic engineering, genes can be inserted
pellet microprojectiles, coated with the from one organism into another or back into the
recombinant DNA molecules, into plant cells in original organism using which of the following
intact tissues, is termed as techniques?
1) Gene gun 2) Microprojection 1) Polymerase chain reaction
3) Biolistics 4) All of these 2) Gene gun
48. A chimaeric DNA is formed by 3) DNA hybridization
1) Deleting selectable markers 4) Gel electrophoresis
2) Joining c-DNA with plasmid 55. Which of the following is/are true?
3) EcoRI
I. Biowar is the use of biological weapons against
4) Enzyme -galactesidase humans and /or their crops and animals.

49. Which of the following was the first artificial II. Bioethics is the unauthorized use of
cloning vector constructed in 1977 by Boliver bioresources and traditional knowledge related
and Rodriguez and widely used in gene cloning to bioresources for commercial benefits.
experiments? III. Biopatent is exploitation of bioresources of
1) pBR322 2) Cosmid other nations without proper authorisation.
3) Phagemid 4) BACs 1) II only 2) I only
3) I and II only 4) I and III only
50. The first recombinant DNA involved the use of a
plasmid obtained from which of the following 56. A single strand of nucleic acid tagged with a
organisms? radioactive molecule is called:
1) Salmonella typhimurium 1) Vector 2) Selectable marker
2) Escherichia coli 3) Plasmid 4) Probe
3)  Bacteriophage 4) Yeast
57. cry II Ab and cry I Ab produce toxins that control:
51. Golden rice is a transgenic crop of the future with 1) Cotton bollworms and corn borer respectively
the following improved trait 2) Cotton borer and cotton bollworms respectively
1) High lysine (essential amino acid) content 3) Tobacco budworms and nematodes
2) Insect resistance respectively
3) High protein content 4) Nematodes and tobacco budworms respectively
4) High vitamin-A content
58. The protein  -1 antitrypsin is used to treat the
52. A genomic library is disease
1) Where you look to find out how to make 1) Cancer
recombinant DNA.
2) Rheumatoid arthritis
2) A listing of the known nucleotide sequences for
a particular species. 3) Alzheimer’s disease
3) All the genes contained in one kind of cell. 4) Emphysema
4) A collection of cloned DNA pieces from an 59. Find out the wrong statement
organism's genome.
1) Human protein used to treat emphysema is
 -1 antitrypsin.
53. In the adjacent identify the ‘marking’ that codes
for proteins involved in the replication of plasmid 2) Human insulin is being commercially
produced from a transgenic species of
Agrobacterium tumifaciens.
3) Rosie, the first transgenic cow, produced
human protein enriched milk.
4) CryI Ab endotoxins obtained from Bacillus
thuringiensis is effective against corn borers.

60. The prerequisites for biotechnological production

of antibiotics is
1) To search an antibiotic producing
microorganism.
2) To isolate the antibiotic gene.
3) To join antibiotic gene with E.coli plasmid.
1) A 2) B 3) C 4) D
4) All of the above

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61. Bt cotton is not 68. Gene amplification using primers can be done by
1) A GM plant 1) Microinjection
2) Insect resistant crop 2) ELISA
3) A bacterial gene expressing system 3) Polymerase chain reaction
4) Resistant to all pests 4) Gene gun
69. Bt toxin is
62. A protoxin is 1) Exotoxin, biodegradable insecticide.
1) A primitive toxin 2) A denatured toxin.
2) Exotoxin nonbiodegradable insecticide.
3) Toxin produced by protozoa.
3) Endotoxin nonbiodegradable insecticide.
4) Inactive toxin.
4) Endotoxin biodegradable insecticide.
63. The trigger for activation of toxin of Bacillus 70. Vector-less transfer of recombinant DNA into a
thuringiensis is plant cell can be accomplished by
1) Acidic pH of stomach 1) BAC 2) Cosmid
2) High temperature 3) Biolistics 4) Ti plasmid
3) Alkaline pH of gut
4) Mechanical action in the insect gut 71. To enable the bacteria to take up recombinant
DNA, it has to be treated with a specific
64. Toxins from Bacillus thuringiensis upon ingestion concentration of
by susceptible insects are converted into active 1) Monovalent cation 2) Monovalent anion
form and kill them by 3) Divalent cation 4) Divalent anion
1) Stopping their digestion and absorption
2) Inhibition of ion transport in the midgut 72. A procedure through which a piece of DNA is
3) Damaging their chromosomes introduced in a host bacterium is called
4) Changing them to mutagenic form 1) Transcription 2) Transformation
3) Transfection 4) Translocation
65. The human protein enriched milk, which
contained the human -lactalbumin was 73. To isolate DNA from an animal cell, it has to be
produced by transgenic treated with
1) Molly 2) Andi 1) Lysozyme 2) Cellulase
3) Rosie 4) George 3) Chitinase 4) Neuraminidase

66. Functional insulin molecule is made up of 74. The diagnostic technique based on antigen-
1) Chain A (21), B (30), C (33) antibody interaction is
2) Chain A (31), C (21) 1) PCR
3) Chain A (21), B (30), 2) ELISA 3) Serum analysis
4) Chain B (33), C (31) 4) Recombinant DNA technology
67. Identify the correct match for the given 75. The genetically modified brinjal in India has been
apparatus. developed for
1) Insect resistance
2) Enhancing shelf life
3) Enhancing mineral content
4) Drought resistance
76. Significant features of recombinant therapeutics
is that it
1) Synthesizes unwanted material
2) Does not evoke unwanted immunological
responses
Sterile Air
3) Induces breeding
Apparatus Function 4) Induces unwanted response
1) Gene gun Vectorless direct gene 77. Although the Ti plasmid has revolutionised plant
transfer genetic engineering one limitation of its use is
2) Column Separation of that it
chromatograph chlorophyll pigments 1) Cannot infect broad leaf plants
3) Stirred tank Carry out fermentation 2) Cannot be used on fruit-bearing plants
bioreactor process 3) Cannot transmit prokaryotic genes
4) Respirometer Finding out rate of 4) Does not infect cereal plants such as corn
respiration and rice

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EXCEL ACADAMICS - Bangalore 6 Biology Assignment
78. If you create a GMO, you will require permission 86. If any protein encoding gene is expressed in a
from which agency of Government of India heterologous host, it is called as a
before you can use it commercially in India? 1) Inducible protein
1) IARI 2) GEAC 2) Nutritive protein
3) CAG 4) CDRI 3) Recombinant protein
79. Which Indian plants have either been either 4) Polymeric protein
patented or attempts have been made to patent 87. Downstream processing by recombination DNA
them by western nations for their commercial technology does not include
use? 1) Separation of a particular antibiotic
1) Basmati rice 2) Purification of a particular antibiotic
2) Turmeric 3) Neem 3) Synthesis of a particular antibiotic
4) All of these have been targetted 4) Addition of preservation to a particular
80. Which advantage of Flavr Savr over the usual antibiotic
tomatoes gives it a commercial reason to be 88. Which of the following are critical research areas
produced by biotechnology? of biotechnology?
1) Relatively larger size 1) Providing the best catalyst in the form of
2) Relatively more lycopene pigment improved organism usually a microbe or pure
3) Relatively longer shelf life enzyme
4) All of these 2) Creating optimal conditions through
engineering for a catalyst to act
81. Some of the steps involved in the production of
3) Downstream processing technologies
humulin are given below. Arrange them in the
correct sequence and select the correct option 4) All of these
i) Synthesis of gene (DNA) for human insulin 89. Taq DNA polymerase used in PCR is obtained
artificially from _______ and active at the temperature of
ii) Culturing recombinant E.coli in bioreactors ________.
iii) Purification of humulin 1) Thermus thermophilus, 35º –50º C
iv) Insertion of human insulin gene into plasmid 2) Thermus aquaticus, 72º – 94ºC
v) Introduction of recombinant plasmid into 3) Haemophilus influenza, 50º – 70ºC
E.coli 4) E. coli, 72º – 94ºC
vi) Extraction of recombinant gene product from
90. Study the given figure carefully and select the
E.coli
incorrect statements regarding this
1) ii, i, iv, iii, v, vi 2) i, iii, v, vi, ii, iv
3) i, iv, v, ii, vi, iii 4) iii, v, ii, i, vi, iv
82. Thermal cycle is utilized in
1) PCR 2) Chemical reaction
3) Radioactivation 4) All of these
83. Low concentration of bacteria or viruses can be
detected by
1) Enzymatic activity
i) It represents a typical agarose gel
2) Body wall electrophoresis in which lane 1 contains
3) Colouration undigested DNA
4) Amplification of the nucleic acid by PCR ii) Smallest DNA bands are formed at A and
largest DNA bands are formed at B
84. The first transgenic crop plant was
iii) The separated DNA fragments can be
1) Cotton 2) Cereals
visualized after staining in the visible light
3) Tobacco 4) Pea
iv) The separated DNA bands are cut out from
85. Methods used to introduce alien DNA into host the agarose gel and extracted from the gel
cells include all except piece. This step is known as elution
1) Micro-injection 1) i and ii
2) Biolistics 2) ii and iii
3) Polymerase chain reaction 3) ii and iv
4) Disarmed pathogen 4) i and iv

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EXCEL ACADAMICS - Bangalore 7 Biology Assignment

ANSWER KEY
1. (3) 11. (3) 21. (3) 31. (4) 41. (3) 51. (4) 61. (4) 71. (3) 81. (3)
2. (3) 12. (2) 22. (3) 32. (4) 42. (3) 52. (4) 62. (4) 72. (2) 82. (1)
3. (3) 13. (2) 23. (2) 33. (2) 43. (2) 53. (1) 63. (3) 73. (4) 83. (4)
4. (3) 14. (1) 24. (3) 34. (3) 44. (1) 54. (2) 64. (2) 74. (2) 84. (3)
5. (4) 15. (3) 25. (2) 35. (1) 45. (3) 55. (2) 65. (3) 75. (1) 85. (3)
6. (4) 16. (1) 26. (3) 36. (4) 46. (4) 56. (4) 66. (3) 76. (2) 86. (3)
7. (4) 17. (1) 27. (1) 37. (2) 47. (4) 57. (1) 67. (3) 77. (4) 87. (3)
8. (3) 18. (2) 28. (4) 38. (1) 48. (2) 58. (4) 68. (3) 78. (2) 88. (4)
9. (1) 19. (2) 29. (4) 39. (3) 49. (1) 59. (2) 69. (4) 79. (4) 89. (2)
10. (4) 20. (2) 30. (2) 40. (1) 50. (1) 60. (4) 70. (3) 80. (3) 90. (2)

HINTS AND SOLUTIONS

1. Answer: (3) a vector for cloning desirable genes into animal
Sol. Restriction Endonuclease used in removal of cells.
nucleotide at end of DNA 11. Answer: (3)
2. Answer: (3) Sol. Palindromic sequences in DNA molecule are group
Sol. In India rice varieties has been estimated 200,000 of bases that forms the same sequence when read
3. Answer: (3) in both forward and backward direction. In the
Sol. RNA interference is the phenomenon of inhibiting given question, only option (3) represents a
activity of gene through production of sense and palindromic sequence
antisense RNA’s, which silences mRNA’s of 12. Answer: (2)
nematode Sol. A. tumifaciens have natural ability to transfer T-
4. Answer: (3) DNA of their Ti plasmids into plant genome upon
Sol. DNA probes are specially constructed sequences infection of cells at the wound site and cause an
that are complementary to the target sequence in unorganised growth of a cell mass known as crown
the sample being tested. The probes bond gall.
(hybridize) with the target sequence wherever it 13. Answer: (2)
occurs in the DNA sample. Sol. Genetic engineering is the manipulation of genes in
5. Answer: (4) vitro. Genetic engineering refers to artificial
Sol. EcoRI is an endonuclease enzyme isolated from synthesis, isolation, modification, combination,
strains of E.coli and a part of restriction modified addition and repair of the genetic material (DNA) to
system. So co part stands for coli. alter the phenotype of the host organism to suit
6. Answer: (4) human needs.
Sol. After heat shock at 42oC again cells should be the Three techniques of genetic engineering are:
subjected to ice treatment. (i) rDNA (recombinant DNA technology)
7. Answer: (4) (ii) gene cloning/gene amplification
Sol. All of these molecules can serve as vectors for (iii) gene therapy
cloned DNA 14. Answer: (1)
8. Answer: (3) Sol. pBR 322 is an artificially constructed vector
Sol. Restriction endonuclease are called “molecular plasmid. It is widely used in gene cloning
scissors or biological scissors”. They recognize experiments.
and cut double stranded DNA at specific points 15. Answer: (3)
called palindromic sequences. Palindromic Sol. A – iv, B – iii, C – ii, D – i
sequence are the ones which read same on both 16. Answer: (1)
the strand in 5`→ 3` direction. Same is true about Sol. In E–colibacteria is used in Genetic engineering
3`→5` direction. (or) r–DNA technology
5`—— GA A T T C —— 3` 17. Answer: (1)
3`—— C T T A A – G —— 5` Sol. Genetic engineered bacteria is used for production
9. Answer: (1) of insulin. On 5th July 1983, the american firm Eli
Sol. Hind II is first restriction endonuclease to cut the Lilly launched humulin (1st genetically engineered
DNA sequence human insulin).
10. Answer: (4) 18. Answer: (2)
Sol. Retrovirus as has the ability to transform normal Sol. Gel electrophoresis is a technique for separation of
cells into cancerous cells. Hence, it can be used as DNA fragments according to their size. DNA is

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EXCEL ACADAMICS - Bangalore 8 Biology Assignment
negatively charged so in gel tank when electric 41. Answer: (3)
current is passed, DNA move towards positive Sol. DNA that precipitates can be removed by Spooling.
electrode. 42. Answer: (3)
19. Answer: (2) Sol. After staining DNA fragments coloured bands are
Sol. A piece of DNA is introduced in a host bacterium visible on agarose gel
called transformation 43. Answer: (2)
20. Answer: (2) Sol. Electrical field is applied to cells increase
Sol. It is tumour inducing plasmid it is a part of genetic permeability of the cell membrane
equipment that agrobacterium tumefaciens 44. Answer: (1)
21. Answer: (3) Sol. Self replication do not depend on host chromosome
Sol. Ability of DNA enters the bacterium through pores 45. Answer: (3)
in cell wall Sol. It increase the efficiency with which DNA entres
22. Answer: (3) 46. Answer: (4)
Sol. It is a neutral insulin used to treat diabetes Sol. Single REN should be used.
23. Answer: (2) 47. Answer: (4)
Sol. GMO used in increasing in food quality, tolerant Sol. Gene gun another name is called biolistic (or) micro
abiotic stress projection
24. Answer: (3) 48. Answer: (2)
Sol. Pseudomonas used in bio remediation of oil spills Sol. Chimaeric DNA is other name of recombinant DNA.
in ocean 49. Answer: (1)
25. Answer: (2) Sol. It is 4.3kb in size.
Sol. A segment of radioactive DNA (or) RNA called 50. Answer: (1)
probe Sol. To which antibiotic resistant gene was incorporated
26. Answer: (3) 51. Answer: (4)
Sol. Crystals of Bt–toxin first produced inactive enter
Sol. i.e,  –carotene – pro–vitamin A.
into host become active form
27. Answer: (1) 52. Answer: (4)
Sol. Invitro culture to produced disease resistant plants Sol. A genomic library is a collection of the total
genomic DNA from a single organism. The DNA is
28. Answer: (4)
stored in a population of identical vectors, each
Sol. Cloning gene to produce multiple copies of genes
containing a different insert of DNA.
29. Answer: (4)
53. Answer: (1)
Sol. C–DNA is derived from mRNA, it contains only
Sol. rop is involved in replication of plasmid
exons
54. Answer: (2)
30. Answer: (2)
Sol. Small titanium (or) gold coated with r–DNA called
Sol. Molecular sticher or glue
genegunor, biolistics
31. Answer: (4)
55. Answer: (2)
Sol. Palindromic sequence read the same the both
Sol. Biological weapons against human and other crops
5'  3'and3 '  5' direction
and animals by biowar
32. Answer: (4) 56. Answer: (4)
Sol. Sticky ends, DNA ligase which help in joint DNA Sol. It is sequence will be usually known.
fragments 57. Answer: (1)
33. Answer: (2) Sol. Bt toxin genes were isolated from Bacillus
Sol. Separation of DNA fragments on the based on size thuringiensis and incorporated into several crop
called geielectrophoresis plants such as cotton. Two cry genes, cry I Ac and
34. Answer: (3) cry II Ab have been incorporated in cotton. The
Sol. Bright orange bands appear when UV–rays are genetically modified crop is called Bt cotton as it
passed contains Bt toxin genes against cotton bollworms.
35. Answer: (1) Similarly, cry I Ab has been introduced in Bt corn to
Sol. Extracted from the gel piece it is called elution protect the same from corn borer.
36. Answer: (4) 58. Answer: (4)
Sol. Ori is a replication site with in the host cells Sol. Emphysema is a chronic disorder in which alveolar
37. Answer: (2) walls are damaged due to which respiratory surface
Sol. A bacteria carry foreign DNA into restriction site is decreased. It caused by cigarette smoking. It can
inside a gene to resist antibiotics be cured by  –1 antitrypsin.
38. Answer: (1) 59. Answer: (2)
Sol. Ampicillin and Tetracycline resistant genes. Sol. Insulin chain A and B are produced separately in
39. Answer: (3) plasmid of E. Coli, extracted and combined by
Sol. Recombinant bacteria are colour less. creating disulfide bond to make it human insulin
(active form) called humulin
40. Answer: (1)
Sol. r–DNA incubated on ice treated with 42ºC then
putting back on ice called heat shock method

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EXCEL ACADAMICS - Bangalore 9 Biology Assignment
60. Answer: (4) Sol. It is able to resist insect damage
Sol. Antibiotics are powerful medicines that fight – 76. Answer: (2)
bacterial infection Sol. It is a type of medicine made of proteins derived
61. Answer: (4) from recombinant organism
Sol. Bt–cotton is genetically modified organisms which 77. Answer: (4)
produce insecticide Sol. Ti plasmid is used as vector in transfer genetic
62. Answer: (4) material in plants
Sol. Bt. First produce protoxin it is a inactive toxin 78. Answer: (2)
63. Answer: (3) Sol. Genetic engineering approval committee
Sol. Activated toxin attached to midgut of epithelial cell 79. Answer: (4)
the release alkaline pH Sol. Basmati rice, Turmeric, Neem are patented by
64. Answer: (2) western nation for commercial use
Sol. Inactive protoxin crystal proteins pass into gut 80. Answer: (3)
where alkaline pH solubilise the crystals and Sol. Gene producing polygalactouronase is inactivated
converts into active toxin which binds to epithelial which causes degradation of cell walls that occurs
cells of midgut and causes pores. Which results in during over–ripening
sweeling of lysis and kills the insect. 81. Answer: (3)
65. Answer: (3) Sol. Synthesis gene → insertion in the plasmid →
Sol. 2.4 grams/ litre introduction of r–DNA in to host → culturing r–DNA
66. Answer: (3) in coli → Extraction → purification
Sol. C–peptide has 35 amino acids which is removed 82. Answer: (1)
67. Answer: (3) Sol. Denatuartion – 94oC, Annealing 55oC, extension –
Sol. Diagram 72oC
68. Answer: (3) 83. Answer: (4)
Sol. Polymerase chain reaction is involved in the Sol. HIV detection is done mutations, cancer etc. are
selective amplification of specific region of a DNA diagnosed where DNA/ RNA probes are used
molecule (gene) and can also be used to generate 84. Answer: (3)
a DNA fragment for cloning. It involves three basic Sol. The first transgenic plant is tobacco
steps; denaturation, annealing and extension. 85. Answer: (3)
69. Answer: (4) Sol. Amplification of DNA
Sol. Bt – toxin produced by Bt. Used as insecticide 86. Answer: (3)
70. Answer: (3) Sol. It is a protein encoded by gene expression of gene
Sol. most widely practiced and translation of m–RNA
71. Answer: (3) 87. Answer: (3)
Sol. Ca++ Sol. Before marketing separation and purification of raw
72. Answer: (2) materials
Sol. If it is into eukaryotic cell it is transfection 88. Answer: (4)
73. Answer: (4) Sol. The use of live organism to produce human
Sol. It is glycoside hydrolase enzymes that cut glycosidic products
linkage 89. Answer: (2)
74. Answer: (2) Sol. 72º – 94ºC
Sol. It is a technique of detecting very small amount of 90. Answer: (2)
protein (Antibody) with the help of peroxidase or Sol. Diagramatic
alkaline phosphatase and stain producing substrate
like 5–amino salicyclic acid.
75. Answer: (1)

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