Pyrethrin Concentration

Initial concentration
Real concentration (C) of pyrethrin (Pyr) in pesticide product:
50% Pyrethrin = 250 mL in 10 L (1,25%)
C = 125 mL . 10-1 L-1 (1,25%)
C = 12,5 mL . L-1 (1,25%)
Density (ρ) of Pyr = 1,0±0,1 mg . mL -1
(assumed as pyrethrin I)
Mass (m) of Pyr in product = ρ . v
= 1,0 g mL-1.12,5 mL
= 12,5 g in 1 L (1,25%)
Assumed concentration (C) of Pyr in each spray:
C Per m2
C product each spray = 4% pesticide + 1% repellent + 95% H2O (not distilled water)
V sprayed = 3150 mL
V pesticide = 120 mL
V pyr 50% = 2,5% x 120 mL
= 3 mL
V pyr 100% = 1,5 mL
Mass pyr = 1,5 g in 3150 mL liquid avery spray ( on 214 m hose long) (0,47 g/L)
Assumption main hose as perimeter of square
Wide area = 53,5 m x 53,5 m
= 2862 m2
c pyr = 1,5 g/2862 m2
= 0,524 mg/m2
Note:
Ignored variable: Nozzle distances
Spray range (m2) in every nozzle
Inhomogeneous of water + product mixture
So, we calculate c pyrethrin in every nozzle for more accurate
C per nozzle
C product each spray = 4% pesticide + 1% repellent + 95% H2O (not distilled water)
V sprayed = 3150 mL
V pesticide = 120 mL
V pyr 50% = 2,5% x 120 mL
= 3 mL
V pyr 100% = 1,5 mL
Mass pyr = 1,5 g in 3150 mL liquid every spray (on 214 m + 98 m) (0,47 g/L)
n nozzle = 99
v sprayed each nozzle = 3150 mL/99
= 31,8 mL product sprayed each nozzle
Assumption: V sprayed of each nozzle is considered same
M pyr/nozzle = 1,5 g/99
= 0,015 g pyrethrin each nozzle
= 15 mg pyrethrin sprayed each nozzle
PESTICIDE SAMPLE
Pyrethrin 50% = 6,0% (pure: 3% or 30 g/L)
PBO = 10,0%
Glycerin = 5,90%
Piper betel EO = 0,10%
Sesame oil = 0.5%
IPA = 77,5%
Price/L = Rp 1,578,192

Calculation
(Concentration of pyrethrin per m2 and every nozzle)
C pesticide = 4% of 3150 mL
Per m2
V pesticide = 120 mL/2862 m2
V pyrethrin 50 = 7,2 mL/2862 m2
m pyr = 7,2 g/2862 m2
= 2,5 mg/m2 (5 times higher than initial concentration)
Per nozzle = 7,2 g/99 nozzle
= 72 mg/nozzle
COMPARATIVE STUDY OF PYRETHRIN CONC.

AQUAPY
Aquapy contain 3% of pyrethrin which is in each litre:
V pyr = 3% x 1000 mL
= 300 mL
ρ pyr = 1.0±0,1 g/mL
m pyr = 300 g (in a litre of product)

Space Spray Outdoor (ultra-low volume aerosol)

Manual instruction: 100 mL undiluted product in 500 m2 (100% product)
Mass pyr =?
Mass = ρ.c.V
= 1.0 g/mL . 3% . 100 mL
=3g
c sprayed = 3 g/500 m2
= 6 mg/m2

Surface Spray
Effective up to 24 hours
Manual instruction: diluted 125 mL of product in 5000 mL for 100 m2 (2,5% product)
Mass pyr =?
m = ρ.c.V
= 1.0 g/mL . 3% . 125mL
= 3,75 g in 5000 mL
c sprayed = 3,75 g in 5000 mL/100 m2
= 37,5 mg in 5000 mL/m2
Effective for 1-2 weeks
Manual instruction: diluted 500 mL of product in 5000 mL for 100 m2 (5% product)
Mass pyr =?
m = ρ.c.V
= 1.0 g/mL . 3% . 500mL
= 15 g in 5000 mL
c sprayed = 15 g in 5000 mL/100 m2
= 150 mg in 5000 mL/m2

Space Spray Indoor (ultra-low volume aerosol)

Manual instruction: undiluted product 100mL/3000m3 (100% product)
Mass pyr =?
Mass = ρ.c.V
= 1.0 g/mL . 3% . 100 mL
=3g
c sprayed = 3 g/3000 m3
= 1 g/m3
NATURAL PYRETHIRN
By Southern AG
Ingredients:
Pyrethrin 0,96%
PBO Technical 9,6%
Other ingredients (contain petroleum distillates) 89,44%

Manual:
Surface spray : Product : Water/oil (1:4); 4,5L/70m2 (wet spray)
V product : 1/5 x 4,5 L
: 900 mL
V pyr : 0,96% . 900 mL
: 8,64 mL
m pyr :ρ.v
: 1,0 g/mL . 8,64 mL
: 8,64 g/4,5L
C pyr : 8,64 g/70 m2
: 0,12 g/m2
: 120 mg/m2

Space spray : Product : water/oil (1:19); 30 mL/28 m3 (misting)

V product : 1/29 x 30 mL
: 1,03 mL
V pyr : 0,96% . 1,03 ml
: 0,01 mL
m pyr :ρ.v
: 1,0 g/mL . 0,01 mL
: 0,01 g
C pyr : 0,01 g/28 m3
: 0,36 mg/m3
PyGANIC

Ingredients:
Pyrethrin 1,4%
Other ingredients 98,6%

Manual:
4,5 L product/ 32000 m2 (for agricultural use only)
v pyr : 1,5% x 4,5 L
: 63 mL
C pyr : 63 mL . 1,0 g mL-1/32000 m2
: 1,9 mg/m2

KATYAYANI
Ingredients:
Pyrethrin 2% (w/w)

Pyrethrum Extract 2% m/m is now the most widely used herbal insecticide for the control of public health pests
by mixing with either diesel oil or kerosene as a space spray. Exclusive Features:- The only natural insecticide
used for fogging in India and abroad. Pyrethrum Extract 2% is an oil based concentrate containing 2% m/m
pyrethrins to be diluted in diesel/ kerosene/ petroleum solvent in the ratio of 1:19. Broad-spectrum activity against
variety of household insects such as mosquitoes, flies, cockroach and bed bugs etc. Low mammalian
toxicity .biodegradable and environment friendly, making it the most preferred choice in public health, indicating
more safety to humans, pest and cattle.

Pyrethrum / Pyrethrin Extract 2% is cost effective all in one pest control solution Best for Household Garden &
Other Domestic Purposes. It is preferred by many Central Government and State Government bodies like NAMP,
Municipal corporations, Army, Navy etc in INDIA.

Pyrethrin is very effective for control of Mosquito , Bedbugs , Flies , Cockroaches , ants, moths, flies , fleas and
many other insects. It is used as a fogging chemical or directly sprayed by mixing it with water for indoors &
outdoor areas in your house , garden , lawn & for Agricultural Purposes.

For Fogging : Pyrethrum Extract 2% should be diluted with kerosene or diesel in the ratio 1:15 and applied as
thermal fog or ultra-low volume aerosol spray. For Domestic Use : it can be directly sprayed by mixing it with
water in appropriate Dose.
Pyrethrin concentration in m3 calculated based on LD50
Stephan Duchon et. Al (2009):
LD50 : 0,28 ng/mg (w mosquito average: 2 mg)
To kill 50% of population/individual need : 0,38 ng/mg
So, to kill 50% mosquito individual need : 0,76 ng

n spot space
Assumption: in m3 every spot is 1 mm3 (equal to mosquito body volume)
In m3, how much spot pyrethrin to be occur ?
n spot space = 1m3/1mm3
= 109 mm3/1 mm3
= 109 spot
n spot surface
Assumption: in m2 every spot is 0,1 mm2
In m3, how much spot pyrethrin to be occur ?
n spot space = 1 m2/0,1 mm2
= 106 mm2/0,1 mm3
= 106 spot

In each spot need 0,76 ng

m pyr/m3 = 0,76 . 10-9 g . 109
= 0,76 g
= 760 mg/m3
m pyr/m2 = 0,76 . 10-9 g . 107
= 0,76 . 10-2 g
= 7,6 mg/m2