WORK PROCEDURE OBSERVATIONS REFERENCES

1. Liquid to liquid Bacterial growth in broths is

Group 14 (Staphylococcus)
- The colour of the
broth seems cloudy
and unclear.
indicated by the
development of a cloudy
appearance. If the newly
inoculated broth looks
cloudy at the start, you will
have no way to determine if
this is due to bacterial
growth during the incubation
period (Milne Library,
2014).

Group 17 (E. coli) : Missing

2. Tilted agar to tilted agar White, moist, glistening

Group 15 (E. coli) growth show that the
- Bacteria growth can probable bacteria used is
be clearly seen on Escherichia coli (Tyagi,
the surface of the 2018).
agar.
- The growth of the
bacteria is known as
the effuse growth.
- The bacteria does not spread evenly
throughout the surface of the agar.
- There seems to be a thicker growth
on one side than the other.

3. Petri dish to tilted agar Abundant, opaque, white

Group 18 (Bacillus Sp.) waxy growth show that the
- Bacteria was probable bacteria used is
transferred from a Bacillus Sp (Tyagi, 2018).
petri dish to a tilted
agar media.
- Bacillus bacteria
growth can be
clearly seen on the
surface of the agar.
- It shows an arborescent growth.
WORK PROCEDURE OBSERVATIONS REFERENCES
1. Streak Method The inoculum is streaked
Group 14 (Staphylococcus) & Group 19 over the agar surface in such
(Bacillus Sp.) a way that it “thins out” the
- Using the continuous bacteria. Some individual
streak method, it can bacterial cells are
be clearly observed separated and well-spaced
that the bacteria
from each other.
grew on the surface
of the agar. As the original sample is
- It also can be seen diluted by streaking it over
that the growth of successive quadrants, the
the bacteria is
number of organisms
considered to be a
decreases. Usually, by the
heavy confluent
growth. third or fourth quadrant,
only a few organisms are
transferred which will give
discrete colony forming
units. (Tankeshwar, 2022)

2. Spread Method A successful spread plate

Group 15 (E. coli) & Group 18 will have a
(Staphylococcus) countable number of
- It can be seen that isolated bacterial colonies
the bacteria grew in evenly distributed on the
the shades of white plate (Iowa State University,
and orange
Unknown Year).
distributed evenly.
- The sizes of the
bacteria seem to
vary from small to
large.

- It forms a circular, unbroken, peripheral

edge (orange ones). While the white
ones tend to form in a rhizoid shape:
rootlike, spreading growth.

3. Pour Method Pour plates allow micro-

Group 16 & Group 17 (E. coli) organisms to grow both on
- The colonies seem to the surface and within the
not grow on the agar, medium. Most of the
probably due to colonies grow within the
several human errors medium and are small in
during the lab work.
size and may be confluent.
The few colonies that grow
on the surface are of the
same size and appearance
as those on a streak plate
(Royal Society of Biology,
2021). An E. coli colony is
off-white or beige in
colour with a shiny
texture. It often looks like
mucus or a cloudy film
over the whole surface of
the plate. An E. coli colony
is slightly raised and has an
entire, fixed margin and a
steady growth pattern,
creating concentric growth
rings in the colony (Claire
Gillespie, 2018).
Keep in mind that all petri dishes need to be incubated upside-down to lessen contamination risk from airborne
particles landing on them and to prevent accumulation of water condensation that could disturb the growth of a
culture.
WORK PROCEDURE OBSERVATIONS REFERENCES

White, moist, glistening

- The bacteria growth show that the
seems to grow probable bacteria used is
on the surface Escherichia coli (Tyagi,
of the agar. 2018).
However, the
amount of
bacteria that
appears is not as
much as expected.
- The growth of the bacteria is
known as the effuse growth.
- The bacteria does not spread
evenly throughout the surface of
the agar.
- There seems to be a thicker
growth on one side than the
other.
WORK PROCEDURE OBSERVATIONS REFERENCES

1. Nutrition Agar Each distinct circular

- Using the streak
method, the result
from experiment
2A is transferred to
a nutrition agar in a
petri dish.
- It can be
clearly seen that the bacteria
grew with the pigment
combination of
orange and white.
- The bacteria grew according to the
streaking motion which is
continuous (zig-zag)
2. Straight Agar : Missing
colony should represent an
individual bacterial cell or
group that has divided
repeatedly. Being kept in
one place, the resulting cells
have accumulated to form a
visible patch. Most bacterial
colonies appear white,
cream, or yellow in color,
and fairly circular in shape
(Science Buddies, 2015).

3. Tilted Nutrition Agar

4. Nutrition Broth : Missing

5. Nutrition Gelatin : Stab cultures have bacteria

introduced via a needle
- The method used is stabbed in agar and usually
the stab method. grow in the stabbed area
- There appear some (Michael T. Madigan,
bacteria inside the 2012).
nutrition gelatin.
- It looks like it has a
crateriform growth:
liquefied surface area is saucer-
shaped
All petri dishes need to be incubated upside-down to lessen contamination risk from airborne
particles landing on them and to prevent accumulation of water condensation that could disturb the
growth of a culture.
WORK PROCEDURE OBSERVATIONS REFERENCES

1. Air Isolation Air bacteria can be found

- It can be seen in places with rooms, such
that the bacteria as offices, classrooms, and
seems to grow hospitals where they
more when the appear most often
petri dish was (Fujiyoshi, 2017).
left open for 10
minutes long (1st
picture)
compared to it being left open for
3 minutes long (2nd picture)
- The bacteria has
a dark brown
pigment at the
center and lighter
on the edges.
- It forms a circular
shape. However, the
appearance of the outer edge of the
colony is described as undulate since
it has wavy indentations.
- The second picture shows little to no
signs of bacterial growth on the
surface of the agar.

2. Table Isolation
- The bacteria
seems to spread The average desk habours
all over the plate. a scary 10 million germs
- It has orange and (Initial, Unknown Year).
white pigments.
- Most of
the
colonies
grew in a rhizoid shape
(rootlike, spreading
growth), and irregular
shape (indented and
peripheral edge)

3. Skin Isolation In total, you have about

- The cotton was 1.8 m2 of skin, and more
swabbed with a than 1.5 trillion (that's a 1
zig-zag motion on with 12 zeros) bacteria live
the left palm and on it. In some wet places,
being transferred tens of millions of
to a plate
microbes live on every
containing agar
square centimetre of skin
with a streak
method. (Micropia, Unknown
- It shows that the bacteria spread Year).
all over the plate.
- It has white and orange pigments.
- The sizes of the colonies vary
from small to large.
- The shapes are mostly circular
with an undulate outer edges.
All petri dishes need to be incubated upside-down to lessen contamination risk from
airborne particles landing on them and to prevent accumulation of water condensation
that could disturb the growth of a culture.