Professional Documents
Culture Documents
Total Synthesis of S Cularine Via Nucleophilic Substitution On A Catechol
Total Synthesis of S Cularine Via Nucleophilic Substitution On A Catechol
Total Synthesis of S Cularine Via Nucleophilic Substitution On A Catechol
org/OrgLett Letter
ABSTRACT: Catechols are part of many essential chemicals and are valuable, typically nucleophilic intermediates used in synthesis.
Here we describe an unexpected transformation in which they play the role of the electrophile in a formal nucleophilic aromatic
substitution. We made this discovery while studying a seven-membered dioxepin ring formation during a synthesis of the
benzyltetrahydroisoquinoline (S)-cularine. We suggest a chain mechanism for this new transformation that is triggered by molecular
oxygen and that propagates an electrophilic ortho-quinone.
Scheme 1. (A) Reversible Redox Exchange That Relates With 5 in hand, we attempted several conditions for its
Catechol, the Semiquinone Radical, and ortho-Quinone direct oxidative cyclization based on our previous work,11 but
and (B) Our Initially Proposed Oxidative Cyclization for unfortunately, all attempts led to intractable mixtures without
the Construction of the Seven-Membered Diaryl Ether in any evidence of cyclization. Consequently, we turned our
(S)-Cularine and the Final Approach with the Discovery of attention to an intermolecular coupling between silyl-
a Formal SNAr on Catechols protected OQ 16 and 4-nitrophenol (17), with the idea of
closing the seven-membered ring via a redox neutral
substitution (Scheme 3).23 Gratifyingly, the catalytic aerobic
coupling of 16 and 17 using CuCl (10 mol %) and N,N′-di-
tert-butyl-ethylenediamine (DBED) (10 mol %) afforded
catechol 3 in 88% yield on a 3 g scale, following a reductive
workup using Na2S2O4.24 Given the well-known challenges of
forming diaryl ethers in complex settings,14 the yield of this
coupling and its completion within 1 h at room temperature
using inexpensive and commercially available reagents is
noteworthy.
Whereas we had initially intended to simply remove the
silyl ether in 3, we were surprised to find that the use of
TBAF in the presence of air afforded dibenzodioxepin 4 in
what appeared to be a redox-neutral substitution. Because 4
was somewhat unstable, we immediately advanced the crude
material by methylation to the corresponding dimethyl ether
(not shown). Under our optimized conditions, this two-step
sequence provided the product in 62% yield; notably, the
cyclization could be run on a gram scale. This led to the
synthesis of (S)-cularine (1) in two straightforward steps,
completing a LLS of 14 steps that proceeded in 15% overall
yield.
Given the redox lability of CATs, the unusually clean
cyclization of 3 caught our attention, and whereas a more in-
depth mechanistic study is ongoing, a series of optimization
studies (Table 1) support our preliminary mechanistic
hypothesis (Scheme 4). The cyclization is redox-neutral and
produces 4-nitrophenol (17) as a byproduct that we can
at C4′ and C8.15 A substituent at C8 precludes the most recover in good yield (Table 1, entry 1).25 At shorter
common syntheses based on Pictet−Spengler or Bischler− reaction times, we observe the product along with uncyclized
Napieralski cyclizations to form the C1−C8a bond. There-
phenol (±)-18 (entry 2); likewise, we see decreased amounts
fore, we devised an alternative that capitalized on Ellman’s
of cyclized product (±)-4 and increased amounts of phenol
chiral sulfonamide technology16 to install the lone chiral
(±)-18 if the reaction is run under an atmosphere of N2
center at C1 and our recently reported modification of the
(entry 3) or buffered with acetic acid (entry 4).26 These
Pomeranz−Fritsch cyclization17 to install the isoquinoline
ring by forming the C4−C4a bond. results are consistent with a rapid desilylation of 3 to produce
Our forward synthesis began by the titanium-mediated 18, followed by a relatively slow autoxidation of the CAT to
condensation of (R)-tert-butylsulfinamide (9) with the TBS- OQ 19 that would be accelerated at an increasing pH
silyl ether of ortho-vanillin 12 to provide (R)-tert-butylsulfinyl (Scheme 4A,B).4,27,28 Once formed, 19 would establish an
imine 13 (Scheme 2B).18 A diastereoselective addition of equilibrium with 20 by forming the seven-membered ring,
Grignard reagent 8 via closed TS-I19 delivered sulfinyl amine consistent with previous nucleophilic substitutions on β-
14 in 87% yield with >20:1 dr on a 9 g scale. Ellman’s substituted OQs.23 But to produce CAT 4, OQ 20 must
auxiliary was then removed to allow reductive alkylation with undergo a 2e− | 2H+ redox exchange with CAT 18 that was
a glyoxal dimethyl acetal (11) before tosyl protection of the initially surprising because it appeared to place the more
amine provided 7 with >99% ee and in 83% yield on a scale electron-rich, methoxy-substituted diaryl ether at C6′ of 4
of 7−9 g.20 To effect the desired Pomeranz−Fritsch and the more electron-withdrawing nitro-substituted diaryl
cyclization, we employed our recently reported conditions,17 ether at C6′ of 19. However, a careful inspection of OQ 20
consisting of 3 equiv of TMS-OTf and 4 equiv of 2,6-lutidine. revealed a puckered conformation of the seven-membered
We were pleased to find that the cyclization of 7 to 15 oxepin29 that prevents the delocalization of the oxygen lone
proceeded in 83% yield on a 14 g scale without erosion of pair into the quinone π-system (Scheme 4C). Consequently,
the ee.21 Subsequent hydrogenation with Pd/C removed the the β-phenoxy substituent, which begins as an electron-
benzyl-protecting group and reduced the C3−C4 double donating group in starting material 18, ends as an inductively
bond to complete the synthesis of phenol 5 in a longest withdrawing group that makes OQ 20 a stronger oxidant
linear sequence (LLS) of seven steps. Beginning from o- than OQ 19. By analogy, CAT 4, possessing the more
vanillin, it proceeded in 42% overall yield on a multigram electron-withdrawing oxygen atom at C6′, is thermodynami-
scale and highlights a concise and stereoselective synthesis of cally favored, creating a driving force for conversion to the
C8-substituted BnTHIQs.22 product and propagation of the chain.3,30
237 https://dx.doi.org/10.1021/acs.orglett.0c04000
Org. Lett. 2021, 23, 236−241
Organic Letters pubs.acs.org/OrgLett Letter
Scheme 2. (A) Retrosynthetic Analysis of the Isoquinoline Core and (B) Synthesis of the Key Tetrahydroisoquinoline
Intermediatea
a
Reagents and conditions: (a) (R)-tert-butylsulfinamide (1.2 equiv), Ti(OEt) 4 (2.0 equiv), THF, rt, overnight, 93%. (b) 4-
Benzyloxybenzylmagnesium chloride (2.0 equiv), CH2Cl2 (0.1 M), −48 °C, 4 h, then rt, 12 h, 87%, >20:1 dr. (c) HCl (2.0 equiv), MeOH, 0
°C, 10 min, then NEt3 (4.0 equiv), glyoxal dimethyl acetal (1.5 equiv), rt, overnight, then NaBH4 (3.0 equiv), 0 °C − rt, 1 h, 95%. (d) TsCl (4.0
equiv), DMAP (20 mol %), NEt3 (10.0 equiv), CH2Cl2, rt, 36 h, 87%. (e) TMSOTf (3.0 equiv), 2,6-lutidine (4.0 equiv), CH2Cl2, 0 °C − rt, 4 h,
83%. (f) 10 wt % Pd/C (20 wt %), H2 (10 psi), MeOH/EtOAc, 88%.
Scheme 3. Completing the Total Synthesis of (S)-Cularine via an Unexpected Redox-Neutral Substitution on Catechola
a
Reagents and conditions: (a) IBX (1.2 equiv), DMF, rt, 3 h, then aq. Na2S2O4, 82%. (b) NaIO4 (1.2 equiv), CH2Cl2/H2O, rt, 1 h. (c) CuCl (10
mol %), DBED (10 mol %), 4-nitrophenol (1.2 equiv), O2 (1 atm), CH2Cl2, rt, 1 h, then aq. Na2S2O4, 88% over two steps. (d) TBAF·3 H2O (2.0
equiv), THF, rt, 18 h, air. (e) MeI (5.0 equiv), Cs2CO3 (5.0 equiv), DMSO, rt, 12 h, 62% over two steps. (f) Lithium naphthalenide (4.0 equiv),
−78 °C, 2 h, 90%. (g) HCHO (2.0 equiv), rt, 4 h, then NaBH4 (5.0 equiv), 0 °C − rt, 1 h, 81%.
238 https://dx.doi.org/10.1021/acs.orglett.0c04000
Org. Lett. 2021, 23, 236−241
Organic Letters pubs.acs.org/OrgLett Letter
Table 1. Screening of Conditions for the Cyclization of the Racemic Catechol Precursora
yield (%)b
entry atmosphere additive time (h) (±)-18 (±)-4
1c air 20 <5 71
2 air 4 20 62
3 N2d 20 57 43
4 air HOAc (5 equiv) 20 55 45
5e air HOAc (5 equiv) 20 34 56
a
Reaction performed on a 0.05 mmol scale. bYield determined by 1H NMR using hexamethylbenzene as the internal standard. c4-nitrophenol was
recovered as its methyl ether after the methylation step. dReaction performed under a balloon of N2. eReaction performed at 50 °C.
Scheme 4. (A) Mechanistic Hypothesis for the Seven-Membered Ring Formation via Formal SNAr on Catechol, (B)
Autoxidation of Catechol 18 to ortho-Quinone 19, and (C) Conformational Analysis of the Proposed Acyclic and Cyclic
ortho-Quinone Intermediates
■
pubs.acs.org/OrgLett Letter
AUTHOR INFORMATION R.; Abramovitch, R. A. J. Am. Chem. Soc. 1994, 116, 9745−9746.
(d) De Lera, A. R.; Saá, J. M.; Suau, R.; Castedo, L. J. Heterocycl.
Corresponding Author
Chem. 1987, 24, 95−100.
Jean-Philip Lumb − Department of Chemistry, McGill (11) (a) Esguerra, K. V. N.; Fall, Y.; Lumb, J.-P. Angew. Chem., Int.
University, Montreal, Quebec H3A 0B8, Canada; Ed. 2014, 53, 5877−5881. (b) Xu, W.; Huang, Z.; Ji, X.; Lumb, J.-P.
orcid.org/0000-0002-9283-1199; Email: jean- ACS Catal. 2019, 9, 3800−3810. (c) Huang, Z.; Lumb, J.-P. Angew.
philip.lumb@mcgill.ca Chem., Int. Ed. 2016, 55, 11543−11547.
(12) For related photocatalytic SNAr on electron-rich arenes, see:
Authors (a) Venditto, N. J.; Nicewicz, D. A. Org. Lett. 2020, 22, 4817−4822.
Zheng Huang − Department of Chemistry, McGill (b) Sandfort, F.; Knecht, T.; Pinkert, T.; Daniliuc, C. G.; Glorius, F.
University, Montreal, Quebec H3A 0B8, Canada J. Am. Chem. Soc. 2020, 142, 6913−6919. (c) Liu, W.; Li, J.;
Querard, P.; Li, C.-J. J. Am. Chem. Soc. 2019, 141, 6755−6764.
Xiang Ji − Department of Chemistry, McGill University,
(d) Huang, H.; Lambert, T. H. Angew. Chem., Int. Ed. 2020, 59,
Montreal, Quebec H3A 0B8, Canada 658−662. (e) Holmberg-Douglas, N.; Nicewicz, D. A. Org. Lett.
Complete contact information is available at: 2019, 21, 7114−7118. (f) Tay, N. E. S.; Nicewicz, D. A. J. Am.
https://pubs.acs.org/10.1021/acs.orglett.0c04000 Chem. Soc. 2017, 139, 16100−16104.
(13) For reviews on related electron- and hole-catalyzed processes,
Notes see: (a) Luca, O. R.; Gustafson, J. L.; Maddox, S. M.; Fenwick, A.
Q.; Smith, D. C. Org. Chem. Front. 2015, 2, 823−848. (b) Studer,
The authors declare no competing financial interest.
■
A.; Curran, D. P. Nat. Chem. 2014, 6, 765−773.
(14) Pitsinos, E. N.; Vidali, V. P.; Couladouros, E. A. Eur. J. Org.
ACKNOWLEDGMENTS Chem. 2011, 2011, 1207−1222.
Financial support was provided by the Natural Sciences and (15) Kametani, T.; Kobari, T.; Fukumoto, K.; Fujihara, M. J. Chem.
Engineering Council (NSERC) of Canada (Discovery Grant Soc. C 1971, 1796−1800.
to J.-P.L.), the Fonds de Recherche du Québec Nature et (16) Robak, M. T.; Herbage, M. A.; Ellman, J. A. Chem. Rev. 2010,
Technologies (FRQNT) (Team Grant to J.-P.L.), and the 110, 3600−3740.
(17) Ji, X.; Huang, Z.; Lumb, J.-P. J. Org. Chem. 2020, 85, 1062−
FRQNT Center for Green Chemistry and Catalysis.
■
1072.
(18) (a) Liu, G.; Cogan, D. A.; Ellman, J. A. J. Am. Chem. Soc.
REFERENCES 1997, 119, 9913−9914. (b) Liu, G.; Cogan, D. A.; Owens, T. D.;
(1) (a) Barner, B. A.; Bongat, A. F. G.; Demchenko, A. V. Tang, T. P.; Ellman, J. A. J. Org. Chem. 1999, 64, 1278−1284.
Catechol. In Encyclopedia of Reagents for Organic Synthesis; Wiley, (19) Cogan, D. A.; Liu, G.; Ellman, J. Tetrahedron 1999, 55,
2009. (b) Tyman, J. H. P. Chapter 14. Synthesis of Natural Phenols 8883−8904.
(and Their Derivatives) of Pharmaceutical, Medicinal or Technical (20) Dragoli, D. R.; Burdett, M. T.; Ellman, J. A. J. Am. Chem. Soc.
Interest. In Studies in Organic Chemistry; Tyman, J. H. P., Ed.; 2001, 123, 10127−10128.
Elsevier: 1996; Vol. 52, pp 558−661. (c) Yang, D.-P.; Ji, H.-F.; (21) The optical rotation of our synthesized cularine (1) is similar
Tang, G.-Y.; Ren, W.; Zhang, H.-Y. Molecules 2007, 12, 878−884. to the optical rotation of the natural sample isolated by Manske (ref
(2) (a) García-Borrón, J. C.; Olivares Sánchez, M. C. Biosynthesis 8b) as well as previously prepared synthetic material from Rodrigues
of Melanins. In Melanins and Melanosomes; Wiley-VCH Verlag and Abramovitch (ref 10c).
GmbH & Co. KGaA: 2011; pp 87−116. (b) Liu, Y.; Ai, K.; Lu, L. (22) (a) Chrzanowska, M.; Rozwadowska, M. D. Chem. Rev. 2004,
Chem. Rev. 2014, 114, 5057−5115. 104, 3341−3370. (b) Chrzanowska, M.; Grajewska, A.;
(3) Ingold, K. U.; Pratt, D. A. Chem. Rev. 2014, 114, 9022−9046. Rozwadowska, M. D. Chem. Rev. 2016, 116, 12369−12465.
(4) Yang, J.; Cohen Stuart, M. A.; Kamperman, M. Chem. Soc. Rev. (23) (a) Huang, Z.; Kwon, O.; Huang, H.; Fadli, A.; Marat, X.;
2014, 43, 8271−8298. Moreau, M.; Lumb, J.-P. Angew. Chem., Int. Ed. 2018, 57, 11963−
(5) Ulas, G.; Lemmin, T.; Wu, Y.; Gassner, G. T.; DeGrado, W. F. 11967. (b) Huang, Z.; Askari, M. S.; Esguerra, K. V. N.; Dai, T.-Y.;
Nat. Chem. 2016, 8, 354. Kwon, O.; Ottenwaelder, X.; Lumb, J.-P. Chem. Sci. 2016, 7, 358−
(6) Lobba, M. J.; Fellmann, C.; Marmelstein, A. M.; Maza, J. C.;
369. (c) Huang, Z.; Kwon, O.; Esguerra, K. V. N.; Lumb, J.-P.
Kissman, E. N.; Robinson, S. A.; Staahl, B. T.; Urnes, C.; Lew, R. J.;
Tetrahedron 2015, 71, 5871−5885.
Mogilevsky, C. S.; Doudna, J. A.; Francis, M. B. ACS Cent. Sci. 2020,
(24) The oxidative cross-coupling of catechols and phenols does
6, 1564−1571.
not proceed when using electron-deficient phenols (e.g., 17) (see ref
(7) There are many well-established 2e− | 2H+ oxidations of CATs
to OQs, mediated by hypervalent iodine, lead, and silver amongst 11a). Therefore, the more direct synthesis of 3 from the catechol of
others (ref 4). There are also many examples of catalyzing the 16 and 17 was not possible.
aerobic oxidation of CATs to OQs, but these examples are limited (25) Optimization studies were carried out using racemic 3, whose
in scope to sterically shielded substrates and are typically part of synthetic procedures are shown in the Supporting Information.
bioinorganic studies. See: Allen, S. E.; Walvoord, R. R.; Padilla- (26) While this experiment was set-up under an inert atmosphere,
Salinas, R.; Kozlowski, M. C. Chem. Rev. 2013, 113, 6234−6458. we did not rigorously exclude O2, and therefore it is likely present,
(8) (a) Castedo, L.; Suau, R. Chapter 6. The Cularine Alkaloids. In but at a lower concentration than our optimized conditions.
The Alkaloids: Chemistry and Pharmacology; Brossi, A., Ed.; (27) Catecholates can air oxidize (autoxidation) to the
Academic Press: 1986; Vol. 29, pp 287−324. (b) Bhacca, N. S.; corresponding ortho-quinones by a variety of pathways, including
Craig, J. C.; Manske, R. H. F.; Roy, S. K.; Shamma, M.; Slusarchyk, single electron transfer to O2 from the corresponding catecholate at
W. A. Tetrahedron 1966, 22, 1467−1475. basic pH (pKa1 for catechol ∼9). The resulting semiquinone radical
(9) (a) Blaschke, G.; Scriba, G. Phytochemistry 1985, 25, 111−113. is readily oxidized to the corresponding ortho-quinone by O2 or
(b) Kametani, T.; Fukumoto, K.; Fujihara, M. J. Chem. Soc. D 1971, superoxide radical anion or by disproportionation. For a complete
352−353. discussion, see: (a) Maier, G. P.; Bernt, C. M.; Butler, A. Biomater.
(10) (a) Jackson, A. H.; Stewart, G. W.; Charnock, G. A.; Martin, Sci. 2018, 6, 332−339. (b) Li, G.; Zhang, H.; Sader, F.; Vadhavkar,
J. A. J. Chem. Soc., Perkin Trans. 1 1974, 1911−1920. (b) Rodrigues, N.; Njus, D. Biochemistry 2007, 46, 6978−6983. (c) Lin, Q.; Li, Q.;
J. A. R.; Abramovitch, R. A.; de Sousa, J. D. F.; Leiva, G. C. J. Org. Batchelor-McAuley, C.; Compton, R. G. J. Phys. Chem. C 2015, 119,
Chem. 2004, 69, 2920−2928. (c) de Sousa, J. D. F.; Rodrigues, J. A. 1489−1495.
240 https://dx.doi.org/10.1021/acs.orglett.0c04000
Org. Lett. 2021, 23, 236−241
Organic Letters pubs.acs.org/OrgLett Letter
241 https://dx.doi.org/10.1021/acs.orglett.0c04000
Org. Lett. 2021, 23, 236−241