Dr. Oeke Yunita, S.Si., M.Si., Apt.

Proses penyalinan atau perbanyakan

rangkaian molekul DNA sehingga
terbentuk duplikasi pada molekul
DNA

TUJUAN
Memastikan ketersediaan &
kontinuitas bahan genetik sebelum
sel mengalami pembelahan

WAKTU REPLIKASI
Pada Tahap S (tahap Sintesis) pada
masa pertumbuhan dalam siklus sel
Organisme eukariotik: Nukleus
 Karakteristik
Karakteristik DNA
Perangkat Replikasi DNA
(prokariotik / eukariotik)
(prokariotik / eukariotik)

Proses Replikasi DNA

(prokariotik / eukariotik)
 Perangkat Replikasi DNA

Template: double stranded DNA

Substrate: dNTP deoxyribonucleotide triphosphates (dNTPs)

Primer: short RNA fragment with a

free 3´-OH end
Enzyme: DNA-dependent DNA
polymerase (DDDP),
other enzymes,
protein factor
 Pol  / primase
Ligase I

Polimerase I = Flap
Pol / pol  endonuclease 1 (FEN-1),
RNAse H-1

Pol / pol 

Minichromosome maintenance protein

complex - Mcm 2-7 (antigen T) Replication
protein A (RPA)
 Players involved in DNA Replication

◼ Origin of Replication Enzymes (Initiator Proteins) recognize the start site on

template DNA and initially open up the DNA (single strands)
◼ Helicases untwist the double helix at the replication forks
◼ Primase begins DNA replication by laying an an RNA primer (10 nt long) for DNA
polymerase to start at
◼ Single-strand binding proteins bind to and stabilize the single-stranded DNA
until it can be used as a template
◼ DNA polymerase polymerizes new DNA in a 5’ to 3’ direction, proofreads the
newly laden DNA, excises error ridden DNA and replaces that DNA with the
correct sequence. Speed/fidelity: mutates 1 X 10-10
◼ Topoisomerase corrects “overwinding” ahead of replication forks by breaking,
swiveling, and rejoining DNA strands
◼ Terminal replication proteins stall replication fork progress for polymeriation to
catch-up
◼ DNA ligase anneals nucleotides together, creating a DNA strand
 TAHAP REPLIKASI DNA
1. Denaturasi (Pemisahan) untaian DNA template
2. Inisiasi
3. Pemanjangan untaian DNA
4. Ligasi fragmen DNA
5. Terminasi
MEKANISME REPLIKASI DNA
MEKANISME REPLIKASI DNA
REPLIKASI DNA Eukariotik
 III III

I I

DNA

Materi handout BIOLOGI SEL, Dr. Oeke Yunita, M.Si., Apt.

 Transkripsi & Translasi Translasi & Transkripsi
VIRUS CLASS IV
EUKARIOTIK
RNA (+) RdRp
RNA-dependent
CYTOPLASM RNA polymerase

RNA (-)

RNA (+)
NUCLEUS TRANSKRIPSI

NUCLEUS

TRANSLASI

CYTOPLASM
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WEBINAR, July 18th 2020, Dr. Oeke Yunita, M.Si., Apt.
 REPLIKASI
CORONAVIRUSES

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WEBINAR, July 18th 2020, Dr. Oeke Yunita, M.Si., Apt.

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WEBINAR, July 18th 2020, Dr. Oeke Yunita, M.Si., Apt.
KETELITIAN REPLIKASI DNA
MEKANISME KOREKSI REPLIKASI DNA