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  • Order of Draw, Stopper Colours, Tube Inversions and Rationale For Collection Order

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    Jen F
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    The document provides guidelines for the correct order of blood draw from patients, including the order of collection tubes, stopper colours, required tube inversions, and rationale. It states: 1) Blood cultures should be drawn first to minimize microbial contamination, followed by coagulation tubes with light blue stoppers as they are most affected by other additives. 2) Plain glass tubes with red stoppers should be drawn before plastic serum tubes to prevent contamination. 3) Tubes containing silica or clot activators, such as gold or red stoppered tubes, should be drawn after coagulation tests to avoid interfering with results.

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    BloodCollectionTubes

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    The document provides guidelines for the correct order of blood draw from patients, including the order of collection tubes, stopper colours, required tube inversions, and rationale. It states: 1) Blood cultures should be drawn first to minimize microbial contamination, followed by coagulation tubes with light blue stoppers as they are most affected by other additives. 2) Plain glass tubes with red stoppers should be drawn before plastic serum tubes to prevent contamination. 3) Tubes containing silica or clot activators, such as gold or red stoppered tubes, should be drawn after coagulation tests to avoid interfering with results.

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    27 views1 page

    Order of Draw, Stopper Colours, Tube Inversions and Rationale For Collection Order

    Uploaded by

    Jen F
    The document provides guidelines for the correct order of blood draw from patients, including the order of collection tubes, stopper colours, required tube inversions, and rationale. It states: 1) Blood cultures should be drawn first to minimize microbial contamination, followed by coagulation tubes with light blue stoppers as they are most affected by other additives. 2) Plain glass tubes with red stoppers should be drawn before plastic serum tubes to prevent contamination. 3) Tubes containing silica or clot activators, such as gold or red stoppered tubes, should be drawn after coagulation tests to avoid interfering with results.

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    © All Rights Reserved
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    Department of Laboratory Services

    Ministry of Health
    Brunei Darussalam

    ORDER OF DRAW, STOPPER COLOURS, TUBE INVERSIONS AND RATIONALE FOR COLLECTION ORDER
    Not following the following order of draw may cause inaccurate test results (especially coagulation results), cross contamination of additives in lab tubes,
    and contamination of blood cultures.
    (Ref: CSLI Standard H3-A6 revision November 2007)

    Order of Draw Tube Stopper Colour Tube Inversion Rationale for the Collection Order

    Blood culture (sterile 8-10X Minimise microbial contamination


    collections)

    Coagulation tubes Light blue 4X The first additive tube in order because all other additives affect
    coagulation tests

    Glass plain tubes Red Nil Prevent contamination by other additives in other tubes. Plain glass tube
    should be drawn before the plastic serum tube with SST.

    Clot activator, silicon coated Red 5X


    (plastic) tubes Filled after coagulation tests because silica particles in the plastic tubes
    activate clotting and affect coagulation tests (carry-over of silica into
    SST clot activator, gel Gold 5X subsequent tubes can be overridden by anticoagulant in them)
    separator tubes

    Heparin tubes Green 8X Causes the least interference in tests other than coagulation tests

    EDTA tubes Lavender 8X Responsible for more carry-over problems than any additive. It elevated
    + + ++
    Na and K levels, chelates and decreases Ca and Fe levels, elevates PT
    and APTT results.

    Oxalate / fluoride tubes Gray 8X Oxalate is used after haematology tube (lavender stopper) because
    oxalate interferes in enzyme reaction, damages cell membranes and
    causes abnormal RBC morphology.

    Extracted from: LI-PHL-1 (PHLEBOTOMY PROCEDURE), Pg. 9, Edition 3, Issue date: 12 July 2012

    This is a controlled document. As such it is subject to change. Please ensure you have the most up-to-date copy at all times.

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