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  • Fish Disease Technique-Disease Diagnostic Methods

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    Pisey Say
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    This document outlines various fish disease diagnostic methods including: 1. Gross examination of external and internal lesions using visual inspection. 2. Examination of wet mounts of skin, gill, and fin biopsies under a light microscope. 3. Bacteriology techniques like bacterial isolation and identification. 4. Virology methods including detecting cytopathic effects, electron microscopy, and PCR. 5. Histopathology procedures and microscopic examination of tissue samples.

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    This document outlines various fish disease diagnostic methods including: 1. Gross examination of external and internal lesions using visual inspection. 2. Examination of wet mounts of skin, gill, and fin biopsies under a light microscope. 3. Bacteriology techniques like bacterial isolation and identification. 4. Virology methods including detecting cytopathic effects, electron microscopy, and PCR. 5. Histopathology procedures and microscopic examination of tissue samples.

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    © All Rights Reserved
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    14 views26 pages

    Fish Disease Technique-Disease Diagnostic Methods

    Uploaded by

    Pisey Say
    This document outlines various fish disease diagnostic methods including: 1. Gross examination of external and internal lesions using visual inspection. 2. Examination of wet mounts of skin, gill, and fin biopsies under a light microscope. 3. Bacteriology techniques like bacterial isolation and identification. 4. Virology methods including detecting cytopathic effects, electron microscopy, and PCR. 5. Histopathology procedures and microscopic examination of tissue samples.

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    © All Rights Reserved
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    of 26

    Fish Disease Technique:

    Disease Diagnostic Methods

    Tirawat Rairat, B.Sc. (Pharm), Ph.D.


    Department of Fishery Biology
    Faculty of Fisheries
    Kasetsart University
    Fish Disease Diagnosis
    1. Gross examination
    2. Light microscope
    3. Bacteriology
    4. Virology
    5. Histopathology
    6. PCR

    2
    Gross Examination

    3
    Gross examination
    (external lesions)

    Exophthalmos/Exophthalmia

    Abdominal distension

    Emaciation
    4
    Gross examination
    (external lesions)

    Gill necrosis

    Skin ulcers
    Skin haemorrhage 5
    Gross examination
    (internal lesions)

    Larval Diphyllobothrium sp. in


    coelomic cavity

    Haemorrhage in muscle and viscera

    Nodular lesions in the posterior kidney


    (due to bacterial kidney disease)
    Anguillicoloides spp. infection in the swim bladder 6
    Light Microscope

    7
    Wet mount
    (skin biopsy)

    8
    Wet mount
    (gill biopsy)

    9
    Wet mount
    (fin biopsy)

    10
    Wet mount
    (tissue squash)

    Normal liver of tilapia

    Granulomas in liver of tilapia infected


    with Francisella spp.
    11
    Blood examination

    12
    Bacteriology

    13
    Bacterial isolation

    14
    Bacterial identification

    15
    Virology

    16
    Cytopathic effects (CPE)

    17
    Electron microscopy

    18
    Histopathology

    19
    Histopathology procedures

    20
    Tetrahymenosis Viral nervous necrosis

    Epitheliocystis Epizootic ulcerative syndrome


    Polymerase Chain
    Reaction (PCR)

    22
    DNA amplification by PCR

    23
    Gel electrophoresis

    24
    Real time PCR

    25
    Real time PCR

    26

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